BAM2RPM

Script

This is an R function that can turn your .bam alignment file into an RPM coverage object that can be loaded into R for plotting, be used to make trackfiles, etc.

#!/usr/bin/Rscrit
library(GenomicAlignments)

bam2RPM <- function(PathToBam, LabelName, OutputFile) {
    ### Read in BamFile 
    H3K4_X1 <- BamFile(PathToBam)
    aln <- readGAlignments(H3K4_X1)
    
    ### Convert to Granges and resize to fragment length
    aln <- as(aln, "GRanges")
    aln <- resize(aln, 150)

    ### Exract coverages per nt
    cov <- coverage(aln)
    nreads <- length(aln)

    ### Function that coverts raw cov to rpm
    rpmfun <- function(x) {
        signif(x/nreads * 10^6, 3)
    }

    ### Run function and convert to RleList
    rpmfile <- lapply(cov, rpmfun)
    rpmfile <- as(rpmfile, "SimpleRleList")
    assign(LabelName, rpmfile)
    
    ### Save output
    save(list=LabelName, file=OutputFile)
}