Data Preparation:
In this Data Preparation phase, the following things: 1. Load the SNP genotypes in .vcf format (vcfR::read.vcfR()) 2. Extract the genotypes into an R-compatible format(vcfR::extract.gt()) 3. Rotate the data into the standard R analysis format(t()) 4. Remove individuals (rows) from the data set that have >50% NAs 5. Remove SNPs (columns) that are fixed 6. Impute remaining NAs (using a for()loop) 7. Save the prepared data as a .csv file for the next step(write.csv())
library(vcfR)
##
## ***** *** vcfR *** *****
## This is vcfR 1.13.0
## browseVignettes('vcfR') # Documentation
## citation('vcfR') # Citation
## ***** ***** ***** *****
library(vegan)
## Loading required package: permute
## Loading required package: lattice
## This is vegan 2.6-4
library(ggplot2)
library(ggpubr)
Confirm your working directory and location of files
getwd()
## [1] "/Users/danyajung/Desktop/R project"
list.files(pattern = "vcf")
## [1] "ALL.chr11_GRCh38.genotypes.20170504.vcf"
## [2] "vcf_num_df.csv"
## [3] "vcf_num_df2.csv"
## [4] "vcf_num.csv"
## [5] "vcf_scaled.csv"
Load the vcf data.
my_vcf = "ALL.chr11_GRCh38.genotypes.20170504.vcf"
vcf <- vcfR::read.vcfR(my_vcf, convertNA = T)
## Scanning file to determine attributes.
## File attributes:
## meta lines: 130
## header_line: 131
## variant count: 3
## column count: 2513
##
Meta line 130 read in.
## All meta lines processed.
## gt matrix initialized.
## Character matrix gt created.
## Character matrix gt rows: 3
## Character matrix gt cols: 2513
## skip: 0
## nrows: 3
## row_num: 0
##
Processed variant: 3
## All variants processed
Get genotype score(allele counts)
vcf_num <- vcfR::extract.gt(vcf,
element = "GT",
IDtoRowNames = F,
as.numeric = T,
convertNA = T)
write.csv(vcf_num, file = "vcf_num.csv", row.names = F)
Confirm presence of file
list.files()
## [1] "1000genomes_people_info2-1.csv"
## [2] "ALL.chr11_GRCh38.genotypes.20170504.vcf"
## [3] "final_report_template.Rmd"
## [4] "R project.Rproj"
## [5] "vcf_num_df.csv"
## [6] "vcf_num_df2.csv"
## [7] "vcf_num.csv"
## [8] "vcf_scaled.csv"
## [9] "Workflow.html"
## [10] "Workflow.Rmd"
##Data preperation
vcf_num_t <- t(vcf_num) #Transpose original VCF orientation to R data frame oriantentation
vcf_num_df <- data.frame(vcf_num_t) #Make into a data frame
sample <- row.names(vcf_num_df) #Get person (sample) names
vcf_num_df <- data.frame(sample, vcf_num_df) #Add sample info to data frame
getwd()
## [1] "/Users/danyajung/Desktop/R project"
write.csv(vcf_num_df, file = "vcf_num_df.csv",
row.names = F)
list.files()
## [1] "1000genomes_people_info2-1.csv"
## [2] "ALL.chr11_GRCh38.genotypes.20170504.vcf"
## [3] "final_report_template.Rmd"
## [4] "R project.Rproj"
## [5] "vcf_num_df.csv"
## [6] "vcf_num_df2.csv"
## [7] "vcf_num.csv"
## [8] "vcf_scaled.csv"
## [9] "Workflow.html"
## [10] "Workflow.Rmd"
Load population meta data
pop_meta <- read.csv(file = "1000genomes_people_info2-1.csv")
names(pop_meta)
## [1] "pop" "super_pop" "sample" "sex" "lat" "lng"
names(vcf_num_df)[1:10]
## [1] "sample" "X1" "X2" "X3" NA NA NA NA
## [9] NA NA
Merge the two sets of data
vcf_num_df2 <- merge(pop_meta,
vcf_num_df,
by = "sample")
nrow(vcf_num_df) == nrow(vcf_num_df2)
## [1] TRUE
Check the names of the new data frame
names(vcf_num_df2)[1:15]
## [1] "sample" "pop" "super_pop" "sex" "lat" "lng"
## [7] "X1" "X2" "X3" NA NA NA
## [13] NA NA NA
invar_omit <- function(x) {
cat("Datafram of dim", dim(x), "processed..\n")
sds <- apply(x, 2, sd, na.rm = T)
i_var0 <- which(sds == 0)
cat(length(i_var0), "columns removed\n")
if(length(i_var0) > 0) {
x <- x[, -i_var0]
}
return(x)
}
vcf_noinvar <- vcf_num_df2
vcf_noinvar[, -c(1:6)] <- invar_omit(vcf_noinvar[, -c(1:6)])
## Datafram of dim 2504 3 processed..
## 0 columns removed
my_meta_N_invar_cols <- 0
find_NAs <- function(x){
NAs_TF <- is.na(x)
i_NA <- which(NAs_TF == TRUE)
N_NA <- length(i_NA)
return(i_NA)
}
N_rows <- nrow(vcf_noinvar)
N_NA <- rep(x = 0, times = N_rows)
N_SNPs <- ncol(vcf_noinvar)
for(i in 1:N_rows) {
i_NA <- find_NAs(vcf_noinvar[i,])
N_NA_i <- length(i_NA)
N_NA[i] <- N_NA_i
}
cutoff50 <- N_SNPs*0.5
percent_NA <- N_NA/N_SNPs * 100
any(percent_NA > 50)
## [1] FALSE
mean(percent_NA)
## [1] 0
my_meta_N_meanNA_rows <- mean(percent_NA)
##Imputation
mean_imputation <- function(df) {
n_cols <- ncol(df)
for(i in 1:n_cols) {
column_i <- df[, i]
mean_i <- mean(column_i, na.rm = T)
NAs_i <- which(is.na(column_i))
N_NAs <- length(NAs_i)
column_i[NAs_i] <- mean_i
df[, i] <- column_i
}
return(df)
}
vcf_noNA <- vcf_noinvar
vcf_noNA[, -c(1:6)]
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vcf_scaled <- vcf_noNA
vcf_scaled[, -c(1:6)] <- scale(vcf_noNA[,-c(1:6)])
vcf_pca <- prcomp(vcf_scaled[, -c(1:6)])
write.csv(vcf_scaled, file = "vcf_scaled.csv")
screeplot(vcf_pca)
PCA_variation <- function(pca_summary, PCs = 2) {
var_explained <- pca_summary$importance[2,1:PCs]*100
var_explained <- round(var_explained, 3)
return(var_explained)
}
vcf_pca_summary <- summary(vcf_pca)
var_out <- PCA_variation(vcf_pca_summary,
PCs = 0)
var_out
## [1] 35.635
N_columns <- ncol(vcf_scaled)
N_columns
## [1] 9
cut_off <- 1/N_columns*100
cut_off
## [1] 11.11111
i_cut_off <- which(var_out < cut_off)
i_cut_off
## integer(0)
i_cut_off <- min(i_cut_off)
## Warning in min(i_cut_off): no non-missing arguments to min; returning Inf
i_cut_off
## [1] Inf
my_meta_N_meanNA_rowsPCs <- i_cut_off
my_meta_var_PC123 <- var_out[c(1,2,3)]
my_meta_var_PC123
## [1] 35.635 NA NA
barplot(var_out,
main = "Percent variation (%) Scree plot",
ylab = "Percent variation (%) explained",
names.arg = 1:length(var_out))
abline(h = cut_off, col = 2, lwd = 2)
abline(v = i_cut_off)
legend("topright",
col = c(2,1),
lty = c(1,1),
legend = c("Vertical line: cutoff",
"Horizontal line: 1st value below cut off"))
## Plot PCA results
vcf_pca_scores <- vegan::scores(vcf_pca)
vcf_pca_scores2 <- data.frame(super_pop = vcf_noNA$super_pop,vcf_pca_scores)
vcf_pca_scores2
## super_pop PC1 PC2 PC3
## site1 EUR -0.2182033 0.023252252 -0.1925196
## site2 EUR -0.2182033 0.023252252 -0.1925196
## site3 EUR -0.2182033 0.023252252 -0.1925196
## site4 EUR -0.2182033 0.023252252 -0.1925196
## site5 EUR -0.2182033 0.023252252 -0.1925196
## site6 EUR -0.2182033 0.023252252 -0.1925196
## site7 EUR -0.2182033 0.023252252 -0.1925196
## site8 EUR -0.2182033 0.023252252 -0.1925196
## site9 EUR -0.2182033 0.023252252 -0.1925196
## site10 EUR -0.2182033 0.023252252 -0.1925196
## site11 EUR -0.2182033 0.023252252 -0.1925196
## site12 EUR -0.2182033 0.023252252 -0.1925196
## site13 EUR -0.2182033 0.023252252 -0.1925196
## site14 EUR -0.2182033 0.023252252 -0.1925196
## site15 EUR -0.2182033 0.023252252 -0.1925196
## site16 EUR -0.2182033 0.023252252 -0.1925196
## site17 EUR -0.2182033 0.023252252 -0.1925196
## site18 EUR -0.2182033 0.023252252 -0.1925196
## site19 EUR -0.2182033 0.023252252 -0.1925196
## site20 EUR -0.2182033 0.023252252 -0.1925196
## site21 EUR -0.2182033 0.023252252 -0.1925196
## site22 EUR -0.2182033 0.023252252 -0.1925196
## site23 EUR -0.2182033 0.023252252 -0.1925196
## site24 EUR -0.2182033 0.023252252 -0.1925196
## site25 EUR -0.2182033 0.023252252 -0.1925196
## site26 EUR -0.2182033 0.023252252 -0.1925196
## site27 EUR -0.2182033 0.023252252 -0.1925196
## site28 EUR -0.2182033 0.023252252 -0.1925196
## site29 EUR -0.2182033 0.023252252 -0.1925196
## site30 EUR -0.2182033 0.023252252 -0.1925196
## site31 EUR -0.2182033 0.023252252 -0.1925196
## site32 EUR -0.2182033 0.023252252 -0.1925196
## site33 EUR -0.2182033 0.023252252 -0.1925196
## site34 EUR -0.2182033 0.023252252 -0.1925196
## site35 EUR -0.2182033 0.023252252 -0.1925196
## site36 EUR -0.2182033 0.023252252 -0.1925196
## site37 EUR -0.2182033 0.023252252 -0.1925196
## site38 EUR -0.2182033 0.023252252 -0.1925196
## site39 EUR -0.2182033 0.023252252 -0.1925196
## site40 EUR -0.2182033 0.023252252 -0.1925196
## site41 EUR -0.2182033 0.023252252 -0.1925196
## site42 EUR -0.2182033 0.023252252 -0.1925196
## site43 EUR -0.2182033 0.023252252 -0.1925196
## site44 EUR -0.2182033 0.023252252 -0.1925196
## site45 EUR -0.2182033 0.023252252 -0.1925196
## site46 EUR -0.2182033 0.023252252 -0.1925196
## site47 EUR -0.2182033 0.023252252 -0.1925196
## site48 EUR -0.2182033 0.023252252 -0.1925196
## site49 EUR -0.2182033 0.023252252 -0.1925196
## site50 EUR -0.2182033 0.023252252 -0.1925196
## site51 EUR -0.2182033 0.023252252 -0.1925196
## site52 EUR -0.2182033 0.023252252 -0.1925196
## site53 EUR -0.2182033 0.023252252 -0.1925196
## site54 EUR -0.2182033 0.023252252 -0.1925196
## site55 EUR -0.2182033 0.023252252 -0.1925196
## site56 EUR -0.2182033 0.023252252 -0.1925196
## site57 EUR -0.2182033 0.023252252 -0.1925196
## site58 EUR -0.2182033 0.023252252 -0.1925196
## site59 EUR -0.2182033 0.023252252 -0.1925196
## site60 EUR -0.2182033 0.023252252 -0.1925196
## site61 EUR -0.2182033 0.023252252 -0.1925196
## site62 EUR -0.2182033 0.023252252 -0.1925196
## site63 EUR -0.2182033 0.023252252 -0.1925196
## site64 EUR -0.2182033 0.023252252 -0.1925196
## site65 EUR -0.2182033 0.023252252 -0.1925196
## site66 EUR -0.2182033 0.023252252 -0.1925196
## site67 EUR -0.2182033 0.023252252 -0.1925196
## site68 EUR -0.2182033 0.023252252 -0.1925196
## site69 EUR -0.2182033 0.023252252 -0.1925196
## site70 EUR -0.2182033 0.023252252 -0.1925196
## site71 EUR 2.4192869 0.001963573 2.4462508
## site72 EUR -0.2182033 0.023252252 -0.1925196
## site73 EUR -0.2182033 0.023252252 -0.1925196
## site74 EUR -0.2182033 0.023252252 -0.1925196
## site75 EUR -0.2182033 0.023252252 -0.1925196
## site76 EUR -0.2182033 0.023252252 -0.1925196
## site77 EUR -0.2182033 0.023252252 -0.1925196
## site78 EUR -0.2182033 0.023252252 -0.1925196
## site79 EUR -0.2182033 0.023252252 -0.1925196
## site80 EUR -0.2182033 0.023252252 -0.1925196
## site81 EUR -0.2182033 0.023252252 -0.1925196
## site82 EUR -0.2182033 0.023252252 -0.1925196
## site83 EUR -0.2182033 0.023252252 -0.1925196
## site84 EUR -0.2182033 0.023252252 -0.1925196
## site85 EUR -0.2182033 0.023252252 -0.1925196
## site86 EUR -0.2182033 0.023252252 -0.1925196
## site87 EUR -0.2182033 0.023252252 -0.1925196
## site88 EUR -0.2182033 0.023252252 -0.1925196
## site89 EUR -0.2182033 0.023252252 -0.1925196
## site90 EUR -0.2182033 0.023252252 -0.1925196
## site91 EUR -0.2182033 0.023252252 -0.1925196
## site92 EUR -0.2182033 0.023252252 -0.1925196
## site93 EUR -0.2182033 0.023252252 -0.1925196
## site94 EUR -0.2182033 0.023252252 -0.1925196
## site95 EUR -0.2182033 0.023252252 -0.1925196
## site96 EUR -0.2182033 0.023252252 -0.1925196
## site97 EUR -0.2182033 0.023252252 -0.1925196
## site98 EUR -0.2182033 0.023252252 -0.1925196
## site99 EUR -0.2182033 0.023252252 -0.1925196
## site100 EUR -0.2182033 0.023252252 -0.1925196
## site101 EUR -0.2182033 0.023252252 -0.1925196
## site102 EUR -0.2182033 0.023252252 -0.1925196
## site103 EUR -0.2182033 0.023252252 -0.1925196
## site104 EUR -0.2182033 0.023252252 -0.1925196
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## site106 EUR -0.2182033 0.023252252 -0.1925196
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## site764 AFR -0.2182033 0.023252252 -0.1925196
## site765 AFR -0.2182033 0.023252252 -0.1925196
## site766 EAS -0.2182033 0.023252252 -0.1925196
## site767 EAS -0.2182033 0.023252252 -0.1925196
## site768 EAS -0.2182033 0.023252252 -0.1925196
## site769 EAS -0.2182033 0.023252252 -0.1925196
## site770 EAS -0.2182033 0.023252252 -0.1925196
## site771 EAS -0.2182033 0.023252252 -0.1925196
## site772 EAS -0.2182033 0.023252252 -0.1925196
## site773 EAS -0.2182033 0.023252252 -0.1925196
## site774 EAS -0.2182033 0.023252252 -0.1925196
## site775 EAS -0.2182033 0.023252252 -0.1925196
## site776 EAS -0.2182033 0.023252252 -0.1925196
## site777 EAS -0.2182033 0.023252252 -0.1925196
## site778 EAS -0.2182033 0.023252252 -0.1925196
## site779 EAS -0.2182033 0.023252252 -0.1925196
## site780 EAS -0.2182033 0.023252252 -0.1925196
## site781 EAS -0.2182033 0.023252252 -0.1925196
## site782 EAS -0.2182033 0.023252252 -0.1925196
## site783 EAS -0.2182033 0.023252252 -0.1925196
## site784 EAS -0.2182033 0.023252252 -0.1925196
## site785 EAS -0.2182033 0.023252252 -0.1925196
## site786 EAS -0.2182033 0.023252252 -0.1925196
## site787 AMR -0.2182033 0.023252252 -0.1925196
## site788 AMR -0.2182033 0.023252252 -0.1925196
## site789 AFR -0.2182033 0.023252252 -0.1925196
## site790 AMR -0.2182033 0.023252252 -0.1925196
## site791 AMR -0.2182033 0.023252252 -0.1925196
## site792 AMR -0.2182033 0.023252252 -0.1925196
## site793 AFR -0.2182033 0.023252252 -0.1925196
## site794 AFR -0.2182033 0.023252252 -0.1925196
## site795 AFR -0.2182033 0.023252252 -0.1925196
## site796 EAS -0.2182033 0.023252252 -0.1925196
## site797 EAS -0.2182033 0.023252252 -0.1925196
## site798 EAS -0.2182033 0.023252252 -0.1925196
## site799 EAS -0.2182033 0.023252252 -0.1925196
## site800 EAS -0.2182033 0.023252252 -0.1925196
## site801 EAS -0.2182033 0.023252252 -0.1925196
## site802 EAS -0.2182033 0.023252252 -0.1925196
## site803 EAS -0.2182033 0.023252252 -0.1925196
## site804 EAS -0.2182033 0.023252252 -0.1925196
## site805 EAS 2.4192869 0.001963573 2.4462508
## site806 EAS -0.2182033 0.023252252 -0.1925196
## site807 EAS -0.2182033 0.023252252 -0.1925196
## site808 EAS -0.2182033 0.023252252 -0.1925196
## site809 EAS -0.2182033 0.023252252 -0.1925196
## site810 EAS -0.2182033 0.023252252 -0.1925196
## site811 EAS -0.2182033 0.023252252 -0.1925196
## site812 EAS -0.2182033 0.023252252 -0.1925196
## site813 AFR -0.2182033 0.023252252 -0.1925196
## site814 AFR -0.2182033 0.023252252 -0.1925196
## site815 AMR -0.2182033 0.023252252 -0.1925196
## site816 AMR -0.2182033 0.023252252 -0.1925196
## site817 AMR -0.2182033 0.023252252 -0.1925196
## site818 EAS -0.2182033 0.023252252 -0.1925196
## site819 EAS -0.2182033 0.023252252 -0.1925196
## site820 EAS -0.2182033 0.023252252 -0.1925196
## site821 EAS -0.2182033 0.023252252 -0.1925196
## site822 EAS -0.2182033 0.023252252 -0.1925196
## site823 EAS -0.2182033 0.023252252 -0.1925196
## site824 EAS -0.2182033 0.023252252 -0.1925196
## site825 EAS -0.2182033 0.023252252 -0.1925196
## site826 EAS -0.2182033 0.023252252 -0.1925196
## site827 EAS -0.2182033 0.023252252 -0.1925196
## site828 EAS -0.2182033 0.023252252 -0.1925196
## site829 EAS -0.2182033 0.023252252 -0.1925196
## site830 EAS -0.2182033 0.023252252 -0.1925196
## site831 EAS -0.2182033 0.023252252 -0.1925196
## site832 EAS -0.2182033 0.023252252 -0.1925196
## site833 EAS -0.2182033 0.023252252 -0.1925196
## site834 EAS -0.2182033 0.023252252 -0.1925196
## site835 EAS -0.2182033 0.023252252 -0.1925196
## site836 EAS -0.2182033 0.023252252 -0.1925196
## site837 EAS -0.2182033 0.023252252 -0.1925196
## site838 EUR -0.2182033 0.023252252 -0.1925196
## site839 EUR -0.2182033 0.023252252 -0.1925196
## site840 EUR -0.2182033 0.023252252 -0.1925196
## site841 EUR -0.2182033 0.023252252 -0.1925196
## site842 EUR -0.2182033 0.023252252 -0.1925196
## site843 EUR -0.2182033 0.023252252 -0.1925196
## site844 EUR -0.2182033 0.023252252 -0.1925196
## site845 EUR -0.2182033 0.023252252 -0.1925196
## site846 EUR -0.2182033 0.023252252 -0.1925196
## site847 EUR -0.2182033 0.023252252 -0.1925196
## site848 EUR -0.2182033 0.023252252 -0.1925196
## site849 EUR -0.2182033 0.023252252 -0.1925196
## site850 EUR -0.2182033 0.023252252 -0.1925196
## site851 EUR -0.2182033 0.023252252 -0.1925196
## site852 EAS -0.2182033 0.023252252 -0.1925196
## site853 AMR -0.2182033 0.023252252 -0.1925196
## site854 AMR -0.2182033 0.023252252 -0.1925196
## site855 AFR 2.4192869 0.001963573 2.4462508
## site856 AFR -0.2182033 0.023252252 -0.1925196
## site857 AMR -0.2182033 0.023252252 -0.1925196
## site858 AMR -0.2182033 0.023252252 -0.1925196
## site859 AMR -0.2182033 0.023252252 -0.1925196
## site860 AMR -0.2182033 0.023252252 -0.1925196
## site861 AMR -0.2182033 0.023252252 -0.1925196
## site862 AMR -0.2182033 0.023252252 -0.1925196
## site863 AMR -0.2182033 0.023252252 -0.1925196
## site864 AMR 2.4192869 0.001963573 2.4462508
## site865 AMR -0.2182033 0.023252252 -0.1925196
## site866 AMR -0.2182033 0.023252252 -0.1925196
## site867 AMR -0.2182033 0.023252252 -0.1925196
## site868 AFR -0.2182033 0.023252252 -0.1925196
## site869 AFR -0.2182033 0.023252252 -0.1925196
## site870 AFR -0.2182033 0.023252252 -0.1925196
## site871 AFR -0.2182033 0.023252252 -0.1925196
## site872 AMR -0.2182033 0.023252252 -0.1925196
## site873 AMR -0.2182033 0.023252252 -0.1925196
## site874 AMR -0.2182033 0.023252252 -0.1925196
## site875 AMR -0.2182033 0.023252252 -0.1925196
## site876 AMR -0.2182033 0.023252252 -0.1925196
## site877 AMR -0.2182033 0.023252252 -0.1925196
## site878 AMR -0.2182033 0.023252252 -0.1925196
## site879 AMR -0.2182033 0.023252252 -0.1925196
## site880 AFR -0.2182033 0.023252252 -0.1925196
## site881 AFR -0.2182033 0.023252252 -0.1925196
## site882 AFR 2.4192869 0.001963573 2.4462508
## site883 AMR 2.4192869 0.001963573 2.4462508
## site884 AFR -0.2182033 0.023252252 -0.1925196
## site885 AFR -0.2182033 0.023252252 -0.1925196
## site886 AFR -0.2182033 0.023252252 -0.1925196
## site887 AFR -0.2182033 0.023252252 -0.1925196
## site888 AFR 2.4192869 0.001963573 2.4462508
## site889 AFR -0.2182033 0.023252252 -0.1925196
## site890 AFR 2.4192869 0.001963573 2.4462508
## site891 AFR -0.2182033 0.023252252 -0.1925196
## site892 AFR -0.2182033 0.023252252 -0.1925196
## site893 AFR -0.2182033 0.023252252 -0.1925196
## site894 AFR -0.2182033 0.023252252 -0.1925196
## site895 AFR -0.2182033 0.023252252 -0.1925196
## site896 AFR -0.2182033 0.023252252 -0.1925196
## site897 AMR -0.2182033 0.023252252 -0.1925196
## site898 AMR -0.2182033 0.023252252 -0.1925196
## site899 EAS -0.2182033 0.023252252 -0.1925196
## site900 EAS -0.2182033 0.023252252 -0.1925196
## site901 EAS -0.2182033 0.023252252 -0.1925196
## site902 EAS -0.2182033 0.023252252 -0.1925196
## site903 EAS -0.2182033 0.023252252 -0.1925196
## site904 EAS -0.2182033 0.023252252 -0.1925196
## site905 EAS -0.2182033 0.023252252 -0.1925196
## site906 EAS -0.2182033 0.023252252 -0.1925196
## site907 EAS -0.2182033 0.023252252 -0.1925196
## site908 EAS -0.2182033 0.023252252 -0.1925196
## site909 EAS -0.2182033 0.023252252 -0.1925196
## site910 EAS -0.2182033 0.023252252 -0.1925196
## site911 EAS -0.2182033 0.023252252 -0.1925196
## site912 EAS -0.2182033 0.023252252 -0.1925196
## site913 EAS -0.2182033 0.023252252 -0.1925196
## site914 EAS -0.2182033 0.023252252 -0.1925196
## site915 EAS -0.2182033 0.023252252 -0.1925196
## site916 EAS -0.2182033 0.023252252 -0.1925196
## site917 EAS -0.2182033 0.023252252 -0.1925196
## site918 EAS -0.2182033 0.023252252 -0.1925196
## site919 EAS -0.2182033 0.023252252 -0.1925196
## site920 EAS -0.2182033 0.023252252 -0.1925196
## site921 EAS -0.2182033 0.023252252 -0.1925196
## site922 EAS -0.2182033 0.023252252 -0.1925196
## site923 EAS -0.2182033 0.023252252 -0.1925196
## site924 EAS -0.2182033 0.023252252 -0.1925196
## site925 EAS -0.2182033 0.023252252 -0.1925196
## site926 EAS -0.2182033 0.023252252 -0.1925196
## site927 EAS -0.2182033 0.023252252 -0.1925196
## site928 EAS -0.2182033 0.023252252 -0.1925196
## site929 EAS -0.2182033 0.023252252 -0.1925196
## site930 EAS -0.2182033 0.023252252 -0.1925196
## site931 EAS -0.2182033 0.023252252 -0.1925196
## site932 EAS -0.2182033 0.023252252 -0.1925196
## site933 EAS -0.2182033 0.023252252 -0.1925196
## site934 AFR 2.4192869 0.001963573 2.4462508
## site935 AFR -0.2182033 0.023252252 -0.1925196
## site936 AMR -0.2182033 0.023252252 -0.1925196
## site937 AFR -0.2182033 0.023252252 -0.1925196
## site938 AFR 2.4192869 0.001963573 2.4462508
## site939 AFR -0.2182033 0.023252252 -0.1925196
## site940 AFR -0.2182033 0.023252252 -0.1925196
## site941 AFR -0.2182033 0.023252252 -0.1925196
## site942 AFR -0.2182033 0.023252252 -0.1925196
## site943 AFR -0.2182033 0.023252252 -0.1925196
## site944 AFR -0.2182033 0.023252252 -0.1925196
## site945 AFR -0.2182033 0.023252252 -0.1925196
## site946 AFR -0.2182033 0.023252252 -0.1925196
## site947 AFR 2.4192869 0.001963573 2.4462508
## site948 AFR -0.2182033 0.023252252 -0.1925196
## site949 AFR -0.2182033 0.023252252 -0.1925196
## site950 AFR -0.2182033 0.023252252 -0.1925196
## site951 AFR 2.4192869 0.001963573 2.4462508
## site952 AFR -0.2182033 0.023252252 -0.1925196
## site953 AFR -0.2182033 0.023252252 -0.1925196
## site954 AFR 2.4192869 0.001963573 2.4462508
## site955 AFR -0.2182033 0.023252252 -0.1925196
## site956 AFR -0.2182033 0.023252252 -0.1925196
## site957 AFR 2.4192869 0.001963573 2.4462508
## site958 AFR -0.2182033 0.023252252 -0.1925196
## site959 SAS -0.2182033 0.023252252 -0.1925196
## site960 SAS -0.2182033 0.023252252 -0.1925196
## site961 SAS -0.2182033 0.023252252 -0.1925196
## site962 SAS -0.2182033 0.023252252 -0.1925196
## site963 AFR 2.4192869 0.001963573 2.4462508
## site964 AFR -0.2182033 0.023252252 -0.1925196
## site965 AFR -0.2182033 0.023252252 -0.1925196
## site966 AFR -0.2182033 0.023252252 -0.1925196
## site967 AFR 2.4192869 0.001963573 2.4462508
## site968 AFR 2.4192869 0.001963573 2.4462508
## site969 AFR 2.4192869 0.001963573 2.4462508
## site970 EAS -0.2182033 0.023252252 -0.1925196
## site971 EAS -0.2182033 0.023252252 -0.1925196
## site972 EAS -0.2182033 0.023252252 -0.1925196
## site973 EAS 2.4192869 0.001963573 2.4462508
## site974 AFR -0.2182033 0.023252252 -0.1925196
## site975 AFR 2.4192869 0.001963573 2.4462508
## site976 AFR -0.2182033 0.023252252 -0.1925196
## site977 AFR -0.2182033 0.023252252 -0.1925196
## site978 AFR 2.4192869 0.001963573 2.4462508
## site979 AFR -0.2182033 0.023252252 -0.1925196
## site980 AFR 2.4192869 0.001963573 2.4462508
## site981 AFR -0.2182033 0.023252252 -0.1925196
## site982 AFR -0.2182033 0.023252252 -0.1925196
## site983 AFR 2.4192869 0.001963573 2.4462508
## site984 AFR 2.4192869 0.001963573 2.4462508
## site985 AFR -0.2182033 0.023252252 -0.1925196
## site986 AFR -0.2182033 0.023252252 -0.1925196
## site987 AFR -0.2182033 0.023252252 -0.1925196
## site988 AFR 2.4192869 0.001963573 2.4462508
## site989 AFR -0.2182033 0.023252252 -0.1925196
## site990 AFR -0.2182033 0.023252252 -0.1925196
## site991 AFR -0.2182033 0.023252252 -0.1925196
## site992 AFR 2.4192869 0.001963573 2.4462508
## site993 AFR 2.4192869 0.001963573 2.4462508
## site994 AFR 2.4192869 0.001963573 2.4462508
## site995 AFR -0.2182033 0.023252252 -0.1925196
## site996 AFR -0.2182033 0.023252252 -0.1925196
## site997 AFR -0.2182033 0.023252252 -0.1925196
## site998 AFR -0.2182033 0.023252252 -0.1925196
## site999 AFR -0.2182033 0.023252252 -0.1925196
## site1000 AFR -0.2182033 0.023252252 -0.1925196
## site1001 SAS -0.2182033 0.023252252 -0.1925196
## site1002 SAS -0.2182033 0.023252252 -0.1925196
## site1003 SAS -0.2182033 0.023252252 -0.1925196
## site1004 SAS -0.2182033 0.023252252 -0.1925196
## site1005 SAS -0.2182033 0.023252252 -0.1925196
## site1006 AFR 2.4192869 0.001963573 2.4462508
## site1007 AFR -0.2182033 0.023252252 -0.1925196
## site1008 AFR -0.2182033 0.023252252 -0.1925196
## site1009 AFR 2.4192869 0.001963573 2.4462508
## site1010 AFR -0.2182033 0.023252252 -0.1925196
## site1011 AFR -0.2182033 0.023252252 -0.1925196
## site1012 AFR -0.2182033 0.023252252 -0.1925196
## site1013 AFR -0.2182033 0.023252252 -0.1925196
## site1014 AFR -3.3974860 -49.838445554 2.5829538
## site1015 AFR -0.2182033 0.023252252 -0.1925196
## site1016 AFR 2.4192869 0.001963573 2.4462508
## site1017 AFR -0.2182033 0.023252252 -0.1925196
## site1018 AFR -0.2182033 0.023252252 -0.1925196
## site1019 AFR -0.2182033 0.023252252 -0.1925196
## site1020 AFR -0.2182033 0.023252252 -0.1925196
## site1021 AFR 2.4192869 0.001963573 2.4462508
## site1022 SAS -0.2182033 0.023252252 -0.1925196
## site1023 SAS -0.2182033 0.023252252 -0.1925196
## site1024 SAS -0.2182033 0.023252252 -0.1925196
## site1025 SAS -0.2182033 0.023252252 -0.1925196
## site1026 SAS -0.2182033 0.023252252 -0.1925196
## site1027 SAS -0.2182033 0.023252252 -0.1925196
## site1028 SAS -0.2182033 0.023252252 -0.1925196
## site1029 SAS -0.2182033 0.023252252 -0.1925196
## site1030 SAS -0.2182033 0.023252252 -0.1925196
## site1031 SAS -0.2182033 0.023252252 -0.1925196
## site1032 AFR -0.2182033 0.023252252 -0.1925196
## site1033 AFR -0.2182033 0.023252252 -0.1925196
## site1034 AFR 2.4192869 0.001963573 2.4462508
## site1035 AFR -0.2182033 0.023252252 -0.1925196
## site1036 AFR 2.4192869 0.001963573 2.4462508
## site1037 AFR -0.2182033 0.023252252 -0.1925196
## site1038 SAS -0.2182033 0.023252252 -0.1925196
## site1039 SAS -0.2182033 0.023252252 -0.1925196
## site1040 SAS -0.2182033 0.023252252 -0.1925196
## site1041 SAS -0.2182033 0.023252252 -0.1925196
## site1042 SAS -0.2182033 0.023252252 -0.1925196
## site1043 SAS -0.2182033 0.023252252 -0.1925196
## site1044 SAS -0.2182033 0.023252252 -0.1925196
## site1045 SAS -0.2182033 0.023252252 -0.1925196
## site1046 SAS -0.2182033 0.023252252 -0.1925196
## site1047 SAS -0.2182033 0.023252252 -0.1925196
## site1048 SAS -0.2182033 0.023252252 -0.1925196
## site1049 SAS -0.2182033 0.023252252 -0.1925196
## site1050 SAS -0.2182033 0.023252252 -0.1925196
## site1051 AFR 2.4192869 0.001963573 2.4462508
## site1052 AFR -0.2182033 0.023252252 -0.1925196
## site1053 AFR 2.4192869 0.001963573 2.4462508
## site1054 AFR -0.2182033 0.023252252 -0.1925196
## site1055 AFR -0.2182033 0.023252252 -0.1925196
## site1056 AFR -0.2182033 0.023252252 -0.1925196
## site1057 SAS -0.2182033 0.023252252 -0.1925196
## site1058 SAS -0.2182033 0.023252252 -0.1925196
## site1059 SAS -0.2182033 0.023252252 -0.1925196
## site1060 SAS 2.4192869 0.001963573 2.4462508
## site1061 SAS -0.2182033 0.023252252 -0.1925196
## site1062 SAS -0.2182033 0.023252252 -0.1925196
## site1063 SAS -0.2182033 0.023252252 -0.1925196
## site1064 SAS -0.2182033 0.023252252 -0.1925196
## site1065 SAS -0.2182033 0.023252252 -0.1925196
## site1066 AFR -0.2182033 0.023252252 -0.1925196
## site1067 AFR -0.2182033 0.023252252 -0.1925196
## site1068 AFR -0.2182033 0.023252252 -0.1925196
## site1069 AFR -0.2182033 0.023252252 -0.1925196
## site1070 AFR -0.2182033 0.023252252 -0.1925196
## site1071 AFR -0.2182033 0.023252252 -0.1925196
## site1072 AFR -0.2182033 0.023252252 -0.1925196
## site1073 AFR -0.2182033 0.023252252 -0.1925196
## site1074 AFR -0.2182033 0.023252252 -0.1925196
## site1075 SAS -0.2182033 0.023252252 -0.1925196
## site1076 SAS -0.2182033 0.023252252 -0.1925196
## site1077 SAS -0.2182033 0.023252252 -0.1925196
## site1078 SAS -0.2182033 0.023252252 -0.1925196
## site1079 SAS -0.2182033 0.023252252 -0.1925196
## site1080 SAS -0.2182033 0.023252252 -0.1925196
## site1081 SAS -0.2182033 0.023252252 -0.1925196
## site1082 SAS -0.2182033 0.023252252 -0.1925196
## site1083 SAS -0.2182033 0.023252252 -0.1925196
## site1084 SAS -0.2182033 0.023252252 -0.1925196
## site1085 SAS 2.4192869 0.001963573 2.4462508
## site1086 SAS -0.2182033 0.023252252 -0.1925196
## site1087 AFR -0.2182033 0.023252252 -0.1925196
## site1088 AFR -0.2182033 0.023252252 -0.1925196
## site1089 AFR -0.2182033 0.023252252 -0.1925196
## site1090 AFR 2.4192869 0.001963573 2.4462508
## site1091 AFR -0.2182033 0.023252252 -0.1925196
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## site1100 AFR 2.4192869 0.001963573 2.4462508
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## site1127 AFR 2.4192869 0.001963573 2.4462508
## site1128 AFR 2.4192869 0.001963573 2.4462508
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## site1132 AFR 2.4192869 0.001963573 2.4462508
## site1133 AFR 2.4192869 0.001963573 2.4462508
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## site1135 AFR 2.4192869 0.001963573 2.4462508
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## site1137 AFR -0.2182033 0.023252252 -0.1925196
## site1138 AFR 2.4192869 0.001963573 2.4462508
## site1139 AFR 2.4192869 0.001963573 2.4462508
## site1140 AFR -0.2182033 0.023252252 -0.1925196
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## site1142 AFR -0.2182033 0.023252252 -0.1925196
## site1143 AFR -0.2182033 0.023252252 -0.1925196
## site1144 AFR 2.4192869 0.001963573 2.4462508
## site1145 SAS -0.2182033 0.023252252 -0.1925196
## site1146 SAS 2.4192869 0.001963573 2.4462508
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## site1154 SAS -0.2182033 0.023252252 -0.1925196
## site1155 AFR -0.2182033 0.023252252 -0.1925196
## site1156 AFR 2.4192869 0.001963573 2.4462508
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## site1158 AFR -0.2182033 0.023252252 -0.1925196
## site1159 AFR -0.2182033 0.023252252 -0.1925196
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## site1161 AFR -0.2182033 0.023252252 -0.1925196
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## site1163 AFR 2.4192869 0.001963573 2.4462508
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## site1169 AFR 2.4192869 0.001963573 2.4462508
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## site1181 AFR -0.2182033 0.023252252 -0.1925196
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## site1183 AFR 2.4192869 0.001963573 2.4462508
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## site1185 AFR -0.2182033 0.023252252 -0.1925196
## site1186 AFR -0.2182033 0.023252252 -0.1925196
## site1187 AFR 2.4192869 0.001963573 2.4462508
## site1188 AFR 2.4192869 0.001963573 2.4462508
## site1189 AFR -0.2182033 0.023252252 -0.1925196
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## site1195 AFR 2.4192869 0.001963573 2.4462508
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## site1212 AFR 2.4192869 0.001963573 2.4462508
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## site1217 AFR 2.4192869 0.001963573 2.4462508
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## site1222 AFR 2.4192869 0.001963573 2.4462508
## site1223 AFR 2.4192869 0.001963573 2.4462508
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## site1227 AFR -0.2182033 0.023252252 -0.1925196
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## site1229 AFR 2.4192869 0.001963573 2.4462508
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## site1234 AFR 2.4192869 0.001963573 2.4462508
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## site1236 AFR 2.4192869 0.001963573 2.4462508
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## site1238 AFR 2.4192869 0.001963573 2.4462508
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## site1245 AFR -0.2182033 0.023252252 -0.1925196
## site1246 AFR 2.4192869 0.001963573 2.4462508
## site1247 AFR -0.2182033 0.023252252 -0.1925196
## site1248 AFR -0.2182033 0.023252252 -0.1925196
## site1249 AFR -0.2182033 0.023252252 -0.1925196
## site1250 AFR -0.2182033 0.023252252 -0.1925196
## site1251 AFR -0.2182033 0.023252252 -0.1925196
## site1252 AFR -0.2182033 0.023252252 -0.1925196
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## site1254 AFR -0.2182033 0.023252252 -0.1925196
## site1255 AFR -0.2182033 0.023252252 -0.1925196
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## site1257 AFR 2.4192869 0.001963573 2.4462508
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## site1259 AFR -0.2182033 0.023252252 -0.1925196
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## site1261 AFR 2.4192869 0.001963573 2.4462508
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## site1265 AFR 2.4192869 0.001963573 2.4462508
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## site1282 AFR 2.4192869 0.001963573 2.4462508
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## site1284 AFR 2.4192869 0.001963573 2.4462508
## site1285 AFR 2.4192869 0.001963573 2.4462508
## site1286 AFR 2.4192869 0.001963573 2.4462508
## site1287 AFR 2.4192869 0.001963573 2.4462508
## site1288 AFR -0.2182033 0.023252252 -0.1925196
## site1289 AFR 2.4192869 0.001963573 2.4462508
## site1290 AFR -0.2182033 0.023252252 -0.1925196
## site1291 AFR 2.4192869 0.001963573 2.4462508
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## site1298 AFR 2.4192869 0.001963573 2.4462508
## site1299 AFR 2.4192869 0.001963573 2.4462508
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## site1301 AFR 2.4192869 0.001963573 2.4462508
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## site1304 AFR 2.4192869 0.001963573 2.4462508
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## site1306 AFR 2.4192869 0.001963573 2.4462508
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## site1733 EUR -0.2182033 0.023252252 -0.1925196
## site1734 EUR -0.2182033 0.023252252 -0.1925196
## site1735 EUR -0.2182033 0.023252252 -0.1925196
## site1736 EUR -0.2182033 0.023252252 -0.1925196
## site1737 EUR -0.2182033 0.023252252 -0.1925196
## site1738 EUR -0.2182033 0.023252252 -0.1925196
## site1739 EUR -0.2182033 0.023252252 -0.1925196
## site1740 EUR -0.2182033 0.023252252 -0.1925196
## site1741 EUR -0.2182033 0.023252252 -0.1925196
## site1742 EUR -0.2182033 0.023252252 -0.1925196
## site1743 EUR -0.2182033 0.023252252 -0.1925196
## site1744 EUR -0.2182033 0.023252252 -0.1925196
## site1745 EUR -0.2182033 0.023252252 -0.1925196
## site1746 EUR -0.2182033 0.023252252 -0.1925196
## site1747 EUR -0.2182033 0.023252252 -0.1925196
## site1748 EUR -0.2182033 0.023252252 -0.1925196
## site1749 EUR -0.2182033 0.023252252 -0.1925196
## site1750 EUR 2.4192869 0.001963573 2.4462508
## site1751 EUR 2.4192869 0.001963573 2.4462508
## site1752 EUR -0.2182033 0.023252252 -0.1925196
## site1753 EUR -0.2182033 0.023252252 -0.1925196
## site1754 EUR -0.2182033 0.023252252 -0.1925196
## site1755 EUR -0.2182033 0.023252252 -0.1925196
## site1756 AFR 2.4192869 0.001963573 2.4462508
## site1757 AFR -0.2182033 0.023252252 -0.1925196
## site1758 AFR -0.2182033 0.023252252 -0.1925196
## site1759 AFR 2.4192869 0.001963573 2.4462508
## site1760 AFR 2.4192869 0.001963573 2.4462508
## site1761 AFR -0.2182033 0.023252252 -0.1925196
## site1762 AFR -0.2182033 0.023252252 -0.1925196
## site1763 AFR 2.4192869 0.001963573 2.4462508
## site1764 AFR -0.2182033 0.023252252 -0.1925196
## site1765 AFR -0.2182033 0.023252252 -0.1925196
## site1766 AFR -0.2182033 0.023252252 -0.1925196
## site1767 AFR -0.2182033 0.023252252 -0.1925196
## site1768 AFR 2.4192869 0.001963573 2.4462508
## site1769 AFR -0.2182033 0.023252252 -0.1925196
## site1770 AFR 2.4192869 0.001963573 2.4462508
## site1771 AFR 2.4192869 0.001963573 2.4462508
## site1772 AFR -0.2182033 0.023252252 -0.1925196
## site1773 AFR -0.2182033 0.023252252 -0.1925196
## site1774 AFR -0.2182033 0.023252252 -0.1925196
## site1775 EAS -0.2182033 0.023252252 -0.1925196
## site1776 EAS -0.2182033 0.023252252 -0.1925196
## site1777 EAS -0.2182033 0.023252252 -0.1925196
## site1778 EAS -0.2182033 0.023252252 -0.1925196
## site1779 EAS -0.2182033 0.023252252 -0.1925196
## site1780 EAS -0.2182033 0.023252252 -0.1925196
## site1781 EAS -0.2182033 0.023252252 -0.1925196
## site1782 EAS -0.2182033 0.023252252 -0.1925196
## site1783 EAS -0.2182033 0.023252252 -0.1925196
## site1784 EAS -0.2182033 0.023252252 -0.1925196
## site1785 EAS -0.2182033 0.023252252 -0.1925196
## site1786 EAS -0.2182033 0.023252252 -0.1925196
## site1787 EAS -0.2182033 0.023252252 -0.1925196
## site1788 EAS -0.2182033 0.023252252 -0.1925196
## site1789 EAS -0.2182033 0.023252252 -0.1925196
## site1790 EAS -0.2182033 0.023252252 -0.1925196
## site1791 EAS -0.2182033 0.023252252 -0.1925196
## site1792 EAS -0.2182033 0.023252252 -0.1925196
## site1793 EAS -0.2182033 0.023252252 -0.1925196
## site1794 EAS -0.2182033 0.023252252 -0.1925196
## site1795 EAS -0.2182033 0.023252252 -0.1925196
## site1796 EAS -0.2182033 0.023252252 -0.1925196
## site1797 EAS -0.2182033 0.023252252 -0.1925196
## site1798 EAS -0.2182033 0.023252252 -0.1925196
## site1799 EAS -0.2182033 0.023252252 -0.1925196
## site1800 EAS -0.2182033 0.023252252 -0.1925196
## site1801 EAS -0.2182033 0.023252252 -0.1925196
## site1802 EAS -0.2182033 0.023252252 -0.1925196
## site1803 EAS -0.2182033 0.023252252 -0.1925196
## site1804 EAS -0.2182033 0.023252252 -0.1925196
## site1805 EAS -0.2182033 0.023252252 -0.1925196
## site1806 EAS -0.2182033 0.023252252 -0.1925196
## site1807 EAS -0.2182033 0.023252252 -0.1925196
## site1808 EAS -0.2182033 0.023252252 -0.1925196
## site1809 EAS -0.2182033 0.023252252 -0.1925196
## site1810 EAS -0.2182033 0.023252252 -0.1925196
## site1811 EAS -0.2182033 0.023252252 -0.1925196
## site1812 EAS -0.2182033 0.023252252 -0.1925196
## site1813 EAS -0.2182033 0.023252252 -0.1925196
## site1814 EAS -0.2182033 0.023252252 -0.1925196
## site1815 EAS -0.2182033 0.023252252 -0.1925196
## site1816 EAS -0.2182033 0.023252252 -0.1925196
## site1817 EAS -0.2182033 0.023252252 -0.1925196
## site1818 EAS -0.2182033 0.023252252 -0.1925196
## site1819 EAS -0.2182033 0.023252252 -0.1925196
## site1820 EAS -0.2182033 0.023252252 -0.1925196
## site1821 EAS -0.2182033 0.023252252 -0.1925196
## site1822 EAS -0.2182033 0.023252252 -0.1925196
## site1823 EAS -0.2182033 0.023252252 -0.1925196
## site1824 EAS -0.2182033 0.023252252 -0.1925196
## site1825 EAS -0.2182033 0.023252252 -0.1925196
## site1826 EAS -0.2182033 0.023252252 -0.1925196
## site1827 EAS -0.2182033 0.023252252 -0.1925196
## site1828 EAS -0.2182033 0.023252252 -0.1925196
## site1829 EAS -0.2182033 0.023252252 -0.1925196
## site1830 EAS -0.2182033 0.023252252 -0.1925196
## site1831 EAS -0.2182033 0.023252252 -0.1925196
## site1832 EAS -0.2182033 0.023252252 -0.1925196
## site1833 EAS -0.2182033 0.023252252 -0.1925196
## site1834 EAS -0.2182033 0.023252252 -0.1925196
## site1835 EAS -0.2182033 0.023252252 -0.1925196
## site1836 EAS -0.2182033 0.023252252 -0.1925196
## site1837 EAS -0.2182033 0.023252252 -0.1925196
## site1838 EAS -0.2182033 0.023252252 -0.1925196
## site1839 EAS -0.2182033 0.023252252 -0.1925196
## site1840 EAS -0.2182033 0.023252252 -0.1925196
## site1841 EAS -0.2182033 0.023252252 -0.1925196
## site1842 EAS -0.2182033 0.023252252 -0.1925196
## site1843 EAS -0.2182033 0.023252252 -0.1925196
## site1844 EAS -0.2182033 0.023252252 -0.1925196
## site1845 EAS -0.2182033 0.023252252 -0.1925196
## site1846 EAS -0.2182033 0.023252252 -0.1925196
## site1847 EAS -0.2182033 0.023252252 -0.1925196
## site1848 EAS -0.2182033 0.023252252 -0.1925196
## site1849 EAS -0.2182033 0.023252252 -0.1925196
## site1850 EAS -0.2182033 0.023252252 -0.1925196
## site1851 EAS -0.2182033 0.023252252 -0.1925196
## site1852 EAS -0.2182033 0.023252252 -0.1925196
## site1853 EAS -0.2182033 0.023252252 -0.1925196
## site1854 EAS -0.2182033 0.023252252 -0.1925196
## site1855 EAS -0.2182033 0.023252252 -0.1925196
## site1856 EAS -0.2182033 0.023252252 -0.1925196
## site1857 EAS -0.2182033 0.023252252 -0.1925196
## site1858 EAS -0.2182033 0.023252252 -0.1925196
## site1859 EAS -0.2182033 0.023252252 -0.1925196
## site1860 EAS -0.2182033 0.023252252 -0.1925196
## site1861 EAS -0.2182033 0.023252252 -0.1925196
## site1862 EAS -0.2182033 0.023252252 -0.1925196
## site1863 EAS -0.2182033 0.023252252 -0.1925196
## site1864 EAS -0.2182033 0.023252252 -0.1925196
## site1865 EAS -0.2182033 0.023252252 -0.1925196
## site1866 EAS -0.2182033 0.023252252 -0.1925196
## site1867 EAS -0.2182033 0.023252252 -0.1925196
## site1868 EAS -0.2182033 0.023252252 -0.1925196
## site1869 EAS -0.2182033 0.023252252 -0.1925196
## site1870 EAS -0.2182033 0.023252252 -0.1925196
## site1871 EAS -0.2182033 0.023252252 -0.1925196
## site1872 EAS -0.2182033 0.023252252 -0.1925196
## site1873 EAS -0.2182033 0.023252252 -0.1925196
## site1874 EAS -0.2182033 0.023252252 -0.1925196
## site1875 EAS -0.2182033 0.023252252 -0.1925196
## site1876 EAS -0.2182033 0.023252252 -0.1925196
## site1877 EAS -0.2182033 0.023252252 -0.1925196
## site1878 AFR -0.2182033 0.023252252 -0.1925196
## site1879 AFR 2.4192869 0.001963573 2.4462508
## site1880 AFR 2.4192869 0.001963573 2.4462508
## site1881 AFR -0.2182033 0.023252252 -0.1925196
## site1882 AFR -0.2182033 0.023252252 -0.1925196
## site1883 AFR -0.2182033 0.023252252 -0.1925196
## site1884 AFR -0.2182033 0.023252252 -0.1925196
## site1885 AFR -0.2182033 0.023252252 -0.1925196
## site1886 AFR -0.2182033 0.023252252 -0.1925196
## site1887 AFR -0.2182033 0.023252252 -0.1925196
## site1888 AFR -0.2182033 0.023252252 -0.1925196
## site1889 AFR -0.2182033 0.023252252 -0.1925196
## site1890 AFR -0.2182033 0.023252252 -0.1925196
## site1891 AFR -0.2182033 0.023252252 -0.1925196
## site1892 AFR 2.4192869 0.001963573 2.4462508
## site1893 AFR 2.4192869 0.001963573 2.4462508
## site1894 AFR -0.2182033 0.023252252 -0.1925196
## site1895 AFR 2.4192869 0.001963573 2.4462508
## site1896 AFR -0.2182033 0.023252252 -0.1925196
## site1897 AFR -0.2182033 0.023252252 -0.1925196
## site1898 AFR -0.2182033 0.023252252 -0.1925196
## site1899 AFR 2.4192869 0.001963573 2.4462508
## site1900 AFR 2.4192869 0.001963573 2.4462508
## site1901 AFR 2.4192869 0.001963573 2.4462508
## site1902 AFR 2.4192869 0.001963573 2.4462508
## site1903 AFR -0.2182033 0.023252252 -0.1925196
## site1904 AFR -0.2182033 0.023252252 -0.1925196
## site1905 AFR -0.2182033 0.023252252 -0.1925196
## site1906 AFR -0.2182033 0.023252252 -0.1925196
## site1907 EAS -0.2182033 0.023252252 -0.1925196
## site1908 EAS -0.2182033 0.023252252 -0.1925196
## site1909 EAS -0.2182033 0.023252252 -0.1925196
## site1910 EAS -0.2182033 0.023252252 -0.1925196
## site1911 EAS -0.2182033 0.023252252 -0.1925196
## site1912 EAS -0.2182033 0.023252252 -0.1925196
## site1913 EAS -0.2182033 0.023252252 -0.1925196
## site1914 EAS -0.2182033 0.023252252 -0.1925196
## site1915 EAS -0.2182033 0.023252252 -0.1925196
## site1916 EAS -0.2182033 0.023252252 -0.1925196
## site1917 EAS -0.2182033 0.023252252 -0.1925196
## site1918 EAS -0.2182033 0.023252252 -0.1925196
## site1919 EAS -0.2182033 0.023252252 -0.1925196
## site1920 EAS -0.2182033 0.023252252 -0.1925196
## site1921 EAS -0.2182033 0.023252252 -0.1925196
## site1922 EAS -0.2182033 0.023252252 -0.1925196
## site1923 EAS -0.2182033 0.023252252 -0.1925196
## site1924 EAS -0.2182033 0.023252252 -0.1925196
## site1925 EAS -0.2182033 0.023252252 -0.1925196
## site1926 EAS -0.2182033 0.023252252 -0.1925196
## site1927 EAS -0.2182033 0.023252252 -0.1925196
## site1928 EAS -0.2182033 0.023252252 -0.1925196
## site1929 EAS -0.2182033 0.023252252 -0.1925196
## site1930 EAS -0.2182033 0.023252252 -0.1925196
## site1931 EAS -0.2182033 0.023252252 -0.1925196
## site1932 EAS -0.2182033 0.023252252 -0.1925196
## site1933 EAS -0.2182033 0.023252252 -0.1925196
## site1934 EAS -0.2182033 0.023252252 -0.1925196
## site1935 EAS -0.2182033 0.023252252 -0.1925196
## site1936 EAS -0.2182033 0.023252252 -0.1925196
## site1937 EAS -0.2182033 0.023252252 -0.1925196
## site1938 EAS -0.2182033 0.023252252 -0.1925196
## site1939 EAS -0.2182033 0.023252252 -0.1925196
## site1940 EAS -0.2182033 0.023252252 -0.1925196
## site1941 EAS -0.2182033 0.023252252 -0.1925196
## site1942 EAS -0.2182033 0.023252252 -0.1925196
## site1943 EAS -0.2182033 0.023252252 -0.1925196
## site1944 EAS -0.2182033 0.023252252 -0.1925196
## site1945 EAS -0.2182033 0.023252252 -0.1925196
## site1946 EAS -0.2182033 0.023252252 -0.1925196
## site1947 EAS -0.2182033 0.023252252 -0.1925196
## site1948 EAS -0.2182033 0.023252252 -0.1925196
## site1949 EAS -0.2182033 0.023252252 -0.1925196
## site1950 EAS -0.2182033 0.023252252 -0.1925196
## site1951 EAS -0.2182033 0.023252252 -0.1925196
## site1952 EAS -0.2182033 0.023252252 -0.1925196
## site1953 EAS -0.2182033 0.023252252 -0.1925196
## site1954 EAS 2.4192869 0.001963573 2.4462508
## site1955 EAS 2.4192869 0.001963573 2.4462508
## site1956 EAS -0.2182033 0.023252252 -0.1925196
## site1957 EAS -0.2182033 0.023252252 -0.1925196
## site1958 EAS -0.2182033 0.023252252 -0.1925196
## site1959 EAS -0.2182033 0.023252252 -0.1925196
## site1960 EAS -0.2182033 0.023252252 -0.1925196
## site1961 EAS -0.2182033 0.023252252 -0.1925196
## site1962 EAS -0.2182033 0.023252252 -0.1925196
## site1963 EAS -0.2182033 0.023252252 -0.1925196
## site1964 EAS -0.2182033 0.023252252 -0.1925196
## site1965 EAS -0.2182033 0.023252252 -0.1925196
## site1966 EAS -0.2182033 0.023252252 -0.1925196
## site1967 EAS -0.2182033 0.023252252 -0.1925196
## site1968 EAS -0.2182033 0.023252252 -0.1925196
## site1969 EAS -0.2182033 0.023252252 -0.1925196
## site1970 EAS 2.4192869 0.001963573 2.4462508
## site1971 EAS -0.2182033 0.023252252 -0.1925196
## site1972 EAS -0.2182033 0.023252252 -0.1925196
## site1973 EAS -0.2182033 0.023252252 -0.1925196
## site1974 EAS -0.2182033 0.023252252 -0.1925196
## site1975 EAS -0.2182033 0.023252252 -0.1925196
## site1976 EAS -0.2182033 0.023252252 -0.1925196
## site1977 AFR 2.4192869 0.001963573 2.4462508
## site1978 AFR -0.2182033 0.023252252 -0.1925196
## site1979 AFR 37.6689747 -4.208685560 -32.8203048
## site1980 AFR 2.4192869 0.001963573 2.4462508
## site1981 AFR -0.2182033 0.023252252 -0.1925196
## site1982 AFR -0.2182033 0.023252252 -0.1925196
## site1983 AFR -0.2182033 0.023252252 -0.1925196
## site1984 AFR -0.2182033 0.023252252 -0.1925196
## site1985 AFR -0.2182033 0.023252252 -0.1925196
## site1986 AFR -0.2182033 0.023252252 -0.1925196
## site1987 AFR -0.2182033 0.023252252 -0.1925196
## site1988 AFR -0.2182033 0.023252252 -0.1925196
## site1989 AFR -0.2182033 0.023252252 -0.1925196
## site1990 AFR -0.2182033 0.023252252 -0.1925196
## site1991 AFR -0.2182033 0.023252252 -0.1925196
## site1992 AFR -0.2182033 0.023252252 -0.1925196
## site1993 AFR -0.2182033 0.023252252 -0.1925196
## site1994 AFR -0.2182033 0.023252252 -0.1925196
## site1995 EAS -0.2182033 0.023252252 -0.1925196
## site1996 EAS -0.2182033 0.023252252 -0.1925196
## site1997 EAS -0.2182033 0.023252252 -0.1925196
## site1998 EAS -0.2182033 0.023252252 -0.1925196
## site1999 EAS -0.2182033 0.023252252 -0.1925196
## site2000 EAS -0.2182033 0.023252252 -0.1925196
## site2001 EAS -0.2182033 0.023252252 -0.1925196
## site2002 EAS -0.2182033 0.023252252 -0.1925196
## site2003 EAS -0.2182033 0.023252252 -0.1925196
## site2004 EAS -0.2182033 0.023252252 -0.1925196
## site2005 EAS -0.2182033 0.023252252 -0.1925196
## site2006 EAS -0.2182033 0.023252252 -0.1925196
## site2007 EAS -0.2182033 0.023252252 -0.1925196
## site2008 EAS -0.2182033 0.023252252 -0.1925196
## site2009 EAS -0.2182033 0.023252252 -0.1925196
## site2010 EAS -0.2182033 0.023252252 -0.1925196
## site2011 EAS -0.2182033 0.023252252 -0.1925196
## site2012 EAS -0.2182033 0.023252252 -0.1925196
## site2013 EAS -0.2182033 0.023252252 -0.1925196
## site2014 EAS -0.2182033 0.023252252 -0.1925196
## site2015 EAS -0.2182033 0.023252252 -0.1925196
## site2016 EAS -0.2182033 0.023252252 -0.1925196
## site2017 EAS -0.2182033 0.023252252 -0.1925196
## site2018 EAS -0.2182033 0.023252252 -0.1925196
## site2019 EAS -0.2182033 0.023252252 -0.1925196
## site2020 EAS -0.2182033 0.023252252 -0.1925196
## site2021 EAS -0.2182033 0.023252252 -0.1925196
## site2022 EAS -0.2182033 0.023252252 -0.1925196
## site2023 EAS -0.2182033 0.023252252 -0.1925196
## site2024 EAS -0.2182033 0.023252252 -0.1925196
## site2025 EAS -0.2182033 0.023252252 -0.1925196
## site2026 EAS -0.2182033 0.023252252 -0.1925196
## site2027 EAS -0.2182033 0.023252252 -0.1925196
## site2028 EAS -0.2182033 0.023252252 -0.1925196
## site2029 AFR -0.2182033 0.023252252 -0.1925196
## site2030 AFR 2.4192869 0.001963573 2.4462508
## site2031 AFR -0.2182033 0.023252252 -0.1925196
## site2032 AFR 2.4192869 0.001963573 2.4462508
## site2033 AFR -0.2182033 0.023252252 -0.1925196
## site2034 AFR -0.2182033 0.023252252 -0.1925196
## site2035 AFR -0.2182033 0.023252252 -0.1925196
## site2036 AFR -0.2182033 0.023252252 -0.1925196
## site2037 AFR 2.4192869 0.001963573 2.4462508
## site2038 AFR -0.2182033 0.023252252 -0.1925196
## site2039 AFR -0.2182033 0.023252252 -0.1925196
## site2040 AFR 2.4192869 0.001963573 2.4462508
## site2041 AFR -0.2182033 0.023252252 -0.1925196
## site2042 AFR 2.4192869 0.001963573 2.4462508
## site2043 AFR 2.4192869 0.001963573 2.4462508
## site2044 AFR -0.2182033 0.023252252 -0.1925196
## site2045 AFR -0.2182033 0.023252252 -0.1925196
## site2046 AFR -0.2182033 0.023252252 -0.1925196
## site2047 AFR -0.2182033 0.023252252 -0.1925196
## site2048 AFR -0.2182033 0.023252252 -0.1925196
## site2049 AFR 2.4192869 0.001963573 2.4462508
## site2050 AFR -0.2182033 0.023252252 -0.1925196
## site2051 AFR -0.2182033 0.023252252 -0.1925196
## site2052 AFR -0.2182033 0.023252252 -0.1925196
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## site2371 EUR -0.2182033 0.023252252 -0.1925196
## site2372 EUR -0.2182033 0.023252252 -0.1925196
## site2373 EUR -0.2182033 0.023252252 -0.1925196
## site2374 EUR -0.2182033 0.023252252 -0.1925196
## site2375 EUR -0.2182033 0.023252252 -0.1925196
## site2376 EUR -0.2182033 0.023252252 -0.1925196
## site2377 EUR -0.2182033 0.023252252 -0.1925196
## site2378 EUR -0.2182033 0.023252252 -0.1925196
## site2379 EUR -0.2182033 0.023252252 -0.1925196
## site2380 EUR -0.2182033 0.023252252 -0.1925196
## site2381 EUR -0.2182033 0.023252252 -0.1925196
## site2382 EUR -0.2182033 0.023252252 -0.1925196
## site2383 EUR -0.2182033 0.023252252 -0.1925196
## site2384 EUR -0.2182033 0.023252252 -0.1925196
## site2385 EUR -0.2182033 0.023252252 -0.1925196
## site2386 EUR -0.2182033 0.023252252 -0.1925196
## site2387 EUR -0.2182033 0.023252252 -0.1925196
## site2388 EUR -0.2182033 0.023252252 -0.1925196
## site2389 EUR -0.2182033 0.023252252 -0.1925196
## site2390 EUR -0.2182033 0.023252252 -0.1925196
## site2391 EUR -0.2182033 0.023252252 -0.1925196
## site2392 EUR -0.2182033 0.023252252 -0.1925196
## site2393 EUR -0.2182033 0.023252252 -0.1925196
## site2394 EUR -0.2182033 0.023252252 -0.1925196
## site2395 EUR -0.2182033 0.023252252 -0.1925196
## site2396 EUR -0.2182033 0.023252252 -0.1925196
## site2397 EUR -0.2182033 0.023252252 -0.1925196
## site2398 EUR -0.2182033 0.023252252 -0.1925196
## site2399 EUR -0.2182033 0.023252252 -0.1925196
## site2400 EUR -0.2182033 0.023252252 -0.1925196
## site2401 EUR -0.2182033 0.023252252 -0.1925196
## site2402 SAS -0.2182033 0.023252252 -0.1925196
## site2403 SAS -0.2182033 0.023252252 -0.1925196
## site2404 SAS -0.2182033 0.023252252 -0.1925196
## site2405 SAS -0.2182033 0.023252252 -0.1925196
## site2406 SAS -0.2182033 0.023252252 -0.1925196
## site2407 SAS -0.2182033 0.023252252 -0.1925196
## site2408 SAS -0.2182033 0.023252252 -0.1925196
## site2409 SAS -0.2182033 0.023252252 -0.1925196
## site2410 SAS -0.2182033 0.023252252 -0.1925196
## site2411 SAS -0.2182033 0.023252252 -0.1925196
## site2412 SAS -0.2182033 0.023252252 -0.1925196
## site2413 SAS -0.2182033 0.023252252 -0.1925196
## site2414 SAS -0.2182033 0.023252252 -0.1925196
## site2415 SAS 2.4192869 0.001963573 2.4462508
## site2416 SAS -0.2182033 0.023252252 -0.1925196
## site2417 SAS -0.2182033 0.023252252 -0.1925196
## site2418 SAS 2.4192869 0.001963573 2.4462508
## site2419 SAS -0.2182033 0.023252252 -0.1925196
## site2420 SAS -0.2182033 0.023252252 -0.1925196
## site2421 SAS -0.2182033 0.023252252 -0.1925196
## site2422 SAS -0.2182033 0.023252252 -0.1925196
## site2423 SAS -0.2182033 0.023252252 -0.1925196
## site2424 SAS -0.2182033 0.023252252 -0.1925196
## site2425 SAS -0.2182033 0.023252252 -0.1925196
## site2426 SAS -0.2182033 0.023252252 -0.1925196
## site2427 SAS -0.2182033 0.023252252 -0.1925196
## site2428 SAS -0.2182033 0.023252252 -0.1925196
## site2429 SAS -0.2182033 0.023252252 -0.1925196
## site2430 SAS -0.2182033 0.023252252 -0.1925196
## site2431 SAS -0.2182033 0.023252252 -0.1925196
## site2432 SAS -0.2182033 0.023252252 -0.1925196
## site2433 SAS -0.2182033 0.023252252 -0.1925196
## site2434 SAS -0.2182033 0.023252252 -0.1925196
## site2435 SAS -0.2182033 0.023252252 -0.1925196
## site2436 SAS -0.2182033 0.023252252 -0.1925196
## site2437 SAS -0.2182033 0.023252252 -0.1925196
## site2438 SAS -0.2182033 0.023252252 -0.1925196
## site2439 SAS -0.2182033 0.023252252 -0.1925196
## site2440 SAS -0.2182033 0.023252252 -0.1925196
## site2441 SAS -0.2182033 0.023252252 -0.1925196
## site2442 SAS -0.2182033 0.023252252 -0.1925196
## site2443 SAS -0.2182033 0.023252252 -0.1925196
## site2444 SAS -0.2182033 0.023252252 -0.1925196
## site2445 SAS -0.2182033 0.023252252 -0.1925196
## site2446 SAS -0.2182033 0.023252252 -0.1925196
## site2447 SAS -0.2182033 0.023252252 -0.1925196
## site2448 SAS -0.2182033 0.023252252 -0.1925196
## site2449 SAS -0.2182033 0.023252252 -0.1925196
## site2450 SAS -0.2182033 0.023252252 -0.1925196
## site2451 SAS -0.2182033 0.023252252 -0.1925196
## site2452 SAS -0.2182033 0.023252252 -0.1925196
## site2453 SAS -0.2182033 0.023252252 -0.1925196
## site2454 SAS -0.2182033 0.023252252 -0.1925196
## site2455 SAS -0.2182033 0.023252252 -0.1925196
## site2456 SAS -0.2182033 0.023252252 -0.1925196
## site2457 SAS -0.2182033 0.023252252 -0.1925196
## site2458 SAS -0.2182033 0.023252252 -0.1925196
## site2459 SAS -0.2182033 0.023252252 -0.1925196
## site2460 SAS -0.2182033 0.023252252 -0.1925196
## site2461 SAS -0.2182033 0.023252252 -0.1925196
## site2462 SAS -0.2182033 0.023252252 -0.1925196
## site2463 SAS -0.2182033 0.023252252 -0.1925196
## site2464 SAS -0.2182033 0.023252252 -0.1925196
## site2465 SAS -0.2182033 0.023252252 -0.1925196
## site2466 SAS -0.2182033 0.023252252 -0.1925196
## site2467 SAS -0.2182033 0.023252252 -0.1925196
## site2468 SAS -0.2182033 0.023252252 -0.1925196
## site2469 SAS -0.2182033 0.023252252 -0.1925196
## site2470 SAS -0.2182033 0.023252252 -0.1925196
## site2471 SAS -0.2182033 0.023252252 -0.1925196
## site2472 SAS -0.2182033 0.023252252 -0.1925196
## site2473 SAS -0.2182033 0.023252252 -0.1925196
## site2474 SAS -0.2182033 0.023252252 -0.1925196
## site2475 SAS -0.2182033 0.023252252 -0.1925196
## site2476 SAS -0.2182033 0.023252252 -0.1925196
## site2477 SAS -0.2182033 0.023252252 -0.1925196
## site2478 SAS -0.2182033 0.023252252 -0.1925196
## site2479 SAS -0.2182033 0.023252252 -0.1925196
## site2480 SAS -0.2182033 0.023252252 -0.1925196
## site2481 SAS -0.2182033 0.023252252 -0.1925196
## site2482 SAS -0.2182033 0.023252252 -0.1925196
## site2483 SAS -0.2182033 0.023252252 -0.1925196
## site2484 SAS -0.2182033 0.023252252 -0.1925196
## site2485 SAS -0.2182033 0.023252252 -0.1925196
## site2486 SAS -0.2182033 0.023252252 -0.1925196
## site2487 SAS -0.2182033 0.023252252 -0.1925196
## site2488 SAS -0.2182033 0.023252252 -0.1925196
## site2489 SAS -0.2182033 0.023252252 -0.1925196
## site2490 SAS -0.2182033 0.023252252 -0.1925196
## site2491 SAS -0.2182033 0.023252252 -0.1925196
## site2492 SAS -0.2182033 0.023252252 -0.1925196
## site2493 SAS -0.2182033 0.023252252 -0.1925196
## site2494 SAS -0.2182033 0.023252252 -0.1925196
## site2495 SAS -0.2182033 0.023252252 -0.1925196
## site2496 SAS -0.2182033 0.023252252 -0.1925196
## site2497 SAS 2.4192869 0.001963573 2.4462508
## site2498 SAS -0.2182033 0.023252252 -0.1925196
## site2499 SAS -0.2182033 0.023252252 -0.1925196
## site2500 SAS -0.2182033 0.023252252 -0.1925196
## site2501 SAS -0.2182033 0.023252252 -0.1925196
## site2502 SAS -0.2182033 0.023252252 -0.1925196
## site2503 SAS -0.2182033 0.023252252 -0.1925196
## site2504 SAS -0.2182033 0.023252252 -0.1925196
my_meta_var_PC123[1]
## [1] 35.635
my_meta_var_PC123[2]
## [1] NA
Plot PC1 vs. PC2
ggpubr::ggscatter(data = vcf_pca_scores2,
y = "PC2",
x = "PC1",
color = "super_pop",
shape = "super_pop",
main = "PCA Scatterplot",
xlab = "PC1 (1.5% of variation)",
ylab = "PC2 (1.1% of variation)")
Plot PC2 vs. PC3
ggpubr::ggscatter(data = vcf_pca_scores2,
y = "PC3",
x = "PC2",
color = "super_pop",
shape = "super_pop",
main = "PCA Scatterplot",
xlab = "PC2 (1.1% of variation)",
ylab = "PC3 (1.05% of variation)")
Plot PC1 VS PC3
ggpubr::ggscatter(data = vcf_pca_scores2,
y = "PC3",
x = "PC1",
color = "super_pop",
shape = "super_pop",
main = "PCA Scatterplot",
xlab = "PC1 (1.5% of variation)",
ylab = "PC3 (1.05% of variation)")