This lesson introduces the concept of invariant columns and why they should be removed. It also provides a function to remove them.
All of this material will appear on the exam. Take notes on the workflow, functions, and concepts.
By the end of this lesson you will
SNPs are Single Nucleotide Polymorphisms. This means that they are by definition polymorphic - more than one nucleotide occurs in that position (locus) in different individual in a species. For example, most individuals in a population may be G|G, but some are G|A or even A|A.
The SNP concept applies at the level of a species. Within separate populations of a species (or individuals genotyped for a study) only one allele of the SNP may be present. This means that though there is genetic variation at that locus in the level of species, for the the population or sample for a study there is no variation.
If all individuals in a population are homozygous for either the major allele (e.g. all are MM), or all are homozygous for the minor allele (e.g. all are mm) we say that the allele is fixed in that population. They other allele is present in other populations, but not all of them. When an allele is fixed in a population it is actually very useful for distinguishing that population genetically from others.
Similarly, for a sample of individuals in a single study, all individuals genotyped may turn out to be the same type of homozygote for a SNP - all are mm or MM. A different sample may have yield heterozygotes or a mix of both types of homozygotes (mm and MM), but for the data at hand, only one type of homozygote is present. While the allele is not necessarily fixed in any population, it is invariant in the individuals that happened to be gentoyped.
SNP data is converted to numeric genotype scores for analysis with machine learning methods such as PCA. If a SNP is invariant in all of the individuals genotyped in a sample for a study, the entire column (aka feature) in the dataframe will be either 0 (all individuals have the major allele) or 2 (all individuals have the minor allele). Similarly, if all individuals are heterozygotes, the entire column will be 1.
Completely invariant alleles in a dataset present 2 issues:
When a column in a dataset is invariant, whether a SNP or anything else, it doesn’t provide you any useful information. For example, if I want to predict whether taking Advanced Placement (AP) Biology predicts success in my intro biology course, and everyone in the class has taken AP Bio, then I can’t learn or conclude anything.
Invariant features, however, still have to be handled by the computer. They therefore eat up computational resources. Since SNP data can have tens or ever hundreds of thousands of features, this can be a major waste of computational time. It is therefore advisable to remove invariant features to speed up the your analyses.
A feature that is invariant will have a standard deviation of 0. For example:
x <- c(2,2,2,2,2,2)
mean(x)## [1] 2sd(x)## [1] 0While we can calculate the mean and standard deviation of an invariant feature, we can’t scale it because this requires division by 0, which is not defined: there is no answer to, for example, x/0.
More generally, machine learning algorithms can run into problems with invariant features and fail to work. It is therefore advisable to remove invariant features before an analysis.
There are several ways to identify if a column in a dataframe is invariant, but probably the easiest is to calculate the standard deviation. The standard deviation is a measure of variation in the data, and if everything in a column is the same, there is no variation and the sd = 0. You could similarly calculate the range; if all the data in a column is 2, then the minimum is 2, the max is 2, and the range is 2 - 2 = 0.
Here’s some example data that shows that this works for all 3 possible forms of invariant SNP genotypic scores data:
First, some fake data. Add c() to make the vectors.
# add the c() function to make all of the vectors
# All samples are homozygous for major allele
SNP_fixed_0 <- c(0, 0, 0, 0, 0, 0) # TODO
# All samples are homozygous for minor allele
SNP_fixed_2 <- c(2, 2, 2, 2, 2, 2) # TODO
# All samples are heterozygous, e.g "A|G"
SNP_all_hetero <- c(1, 1, 1, 1, 1, 1) # TODONow the standard deviations with sd():
# add sd() to calculate the SDs
sd(SNP_fixed_0) # TODO## [1] 0sd(SNP_fixed_2)## [1] 0sd(SNP_all_hetero)## [1] 0Now let’s make a larger dataframe with some features that aren’t invariant.
First, some invariant features:
# Mixture of the 2 homozygotes
SNP_mix_vs1 <- c(0, 0, 0, 2, 2, 2)
# Mixture of the 2 homozygotes
## and heterozygotes
SNP_mix_vs2 <- c(0, 0, 1, 1, 2, 2)We put these into a dataframe:
# add data.frame() and assign to an object
## called df to look at the data
df <- data.frame(SNP_fixed_0, # TODO
SNP_fixed_2,
SNP_mix_vs1,
SNP_mix_vs2)
df## SNP_fixed_0 SNP_fixed_2 SNP_mix_vs1 SNP_mix_vs2
## 1 0 2 0 0
## 2 0 2 0 0
## 3 0 2 0 1
## 4 0 2 2 1
## 5 0 2 2 2
## 6 0 2 2 2To identify which columns are invariant we first calculate all the SDs.
# add sd() to calculate the SDs
# add [, 1] etc to each line
## lines to calculate the sd on each column
## The first one is shown as an exame
#add [, 1]
sd_col01 <- sd(df[,1]) # EXAMPLE
#add [, 2]
sd_col02 <- sd(df[,2]) # TODO
#add [, 3]
sd_col03 <- sd(df[,3]) # TODO
#add [, 4]
sd_col04 <- sd(df[,4]) # TODOWe can put this all into a vector that contains the SDs:
# add c() to make into a vector
## assign it to a vector called sd_vector
sd_vector <- c(sd_col01, # TODO
sd_col02,
sd_col03,
sd_col04)Now use which() to see which parts of the vector are
equal to 0.
# add which() and == 0
which(sd_vector == 0 ) # TODO## [1] 1 2We’ll save this to a vector to hold the indices of the invariant columns.
# assign to a vector called i_invariant
i_invariant <- which(sd_vector == 0) # TODOThis vector of indices can be used to look at which columns are invariant in our dataframe:
# add [,i_invariant] to see
## the invariant columns
df[,i_invariant] # TODO## SNP_fixed_0 SNP_fixed_2
## 1 0 2
## 2 0 2
## 3 0 2
## 4 0 2
## 5 0 2
## 6 0 2We can then use this to show use which columns DO vary by using negative indexing to remove the invariant columns.
# add -i_invariant to REMOVE the invariant columns
df[,-i_invariant] # TODO## SNP_mix_vs1 SNP_mix_vs2
## 1 0 0
## 2 0 0
## 3 0 1
## 4 2 1
## 5 2 2
## 6 2 2We can then make a new dataframe of just the variant columns
# assign to a dataframe called df03
df03 <- df[,-i_invariant] #TODOLook at the results:
df03## SNP_mix_vs1 SNP_mix_vs2
## 1 0 0
## 2 0 0
## 3 0 1
## 4 2 1
## 5 2 2
## 6 2 2The function below will remove invariant columns from a dataframe, as
long as you add return(x) to the end.
# run this function
## add return(x_no_invar)
invar_omit <- function(x){
cat("Dataframe of dim",dim(x), "processed...\n")
sds <- apply(x, 2, sd, na.rm = TRUE)
i_var0 <- which(sds == 0)
cat(length(i_var0),"columns removed\n")
if(length(i_var0) > 0){
x <- x[, -i_var0]
}
## add return() with x in it
return(x) #TODO
}
warning("Reminder: Did you add return() to the function?")## Warning: Reminder: Did you add return() to the function?We can test it on our data above.
# run invar_omit() on df
invar_omit(df) # TODO## Dataframe of dim 6 4 processed...
## 2 columns removed## SNP_mix_vs1 SNP_mix_vs2
## 1 0 0
## 2 0 0
## 3 0 1
## 4 2 1
## 5 2 2
## 6 2 2It should only change data dataframe when columns are invariant. Its always good to test a function to make sure it does what you want. If we run it once, save the output, and run it again on the output we just made, it shouldn’t remove anything the second time.
# run invar_omit() on df
df_no_invar <- invar_omit(df) # TODO## Dataframe of dim 6 4 processed...
## 2 columns removed# look out output
df_no_invar## SNP_mix_vs1 SNP_mix_vs2
## 1 0 0
## 2 0 0
## 3 0 1
## 4 2 1
## 5 2 2
## 6 2 2# run invar_omit() on df_no_invar
invar_omit(df_no_invar) # TODO## Dataframe of dim 6 2 processed...
## 0 columns removed## SNP_mix_vs1 SNP_mix_vs2
## 1 0 0
## 2 0 0
## 3 0 1
## 4 2 1
## 5 2 2
## 6 2 2Let’s work through an example with a real VCF file. The steps we need to follow are
vcfR package..vcffile is present with
list.files()vcfR::read.vcfR()vcfR::extract.gt()t()invar_omit()First, load up the vcfR package with library() and check
the working directory with getwd().
# call library() on vcfR
library(vcfR) #TODO##
## ***** *** vcfR *** *****
## This is vcfR 1.13.0
## browseVignettes('vcfR') # Documentation
## citation('vcfR') # Citation
## ***** ***** ***** *****# check your working directory with getwd()
getwd() #TODO## [1] "/Users/harshitakadiyala/Documents/BIOSC 1540"Second, set the working directory to the location of this file if it isn’t already. Do this via
Session->Set Working Directory -> To source file location
Third, make sure the file all_loci.vcf is present with
list.files() and/or
list.files(pattern = "vcf").
# call list.files()
list.files() #TODO## [1] "all_loci.vcf"
## [2] "Assignment #2.Rmd"
## [3] "Assignment--2_files"
## [4] "center_function.R"
## [5] "feature_engineering_intro_2_functions-part2.Rmd"
## [6] "feature_engineering.Rmd"
## [7] "Mult.java"
## [8] "removing_fixed_alleles.Rmd"
## [9] "RPubs Portfolio.Rmd"
## [10] "RPubs-Portfolio.html"
## [11] "rsconnect"
## [12] "temp.html"
## [13] "temp.Rmd"
## [14] "The vegan package for PCA.Rmd"
## [15] "The-vegan-package-for-PCA.html"
## [16] "transpose_VCF_data.html"
## [17] "transpose_VCF_data.Rmd"
## [18] "Under the Hood of PCA.Rmd"
## [19] "Under-the-Hood-of-PCA_files"
## [20] "Untitled.Rmd"
## [21] "vegan_PCA_amino_acids-STUDENT (1).Rmd"
## [22] "vegan_PCA_amino_acids-STUDENT--1-_files"
## [23] "walsh2017morphology.csv"
## [24] "walsh2017morphology.numbers"
## [25] "working_directory_practice.html"
## [26] "working_directory_practice.Rmd"# call list.files(pattern = "vcf")
list.files(pattern = "vcf") #TODO## [1] "all_loci.vcf"warning("Friendly remindeR: Make sure you have set your working directory")## Warning: Friendly remindeR: Make sure you have set your working directoryFourth, load the .vcf file.
# call vcfR::read.vcfR()
bird_snps_again <- vcfR::read.vcfR("all_loci.vcf",
convertNA = T) #TODO## Scanning file to determine attributes.
## File attributes:
## meta lines: 8
## header_line: 9
## variant count: 1929
## column count: 81
##
Meta line 8 read in.
## All meta lines processed.
## gt matrix initialized.
## Character matrix gt created.
## Character matrix gt rows: 1929
## Character matrix gt cols: 81
## skip: 0
## nrows: 1929
## row_num: 0
##
Processed variant 1000
Processed variant: 1929
## All variants processedwarning("RemindeR: If this didn't work, you may not have set your working directory to the location of the vcf file")## Warning: RemindeR: If this didn't work, you may not have set your working
## directory to the location of the vcf fileFifth, convert to numeric genotypic score (counts of the number of
the minor allele) with vcfR::extract.gt().
# call vcfR::extract.gt()
bird_snps_num_again <- vcfR::extract.gt(bird_snps_again, # TODO
element = "GT",
IDtoRowNames = F,
as.numeric = T,
convertNA = T)Sixth, transpose the data into the format that works with R with
t().
# call t() on bird_snps_num_again
bird_snps_num_t_again <- t(bird_snps_num_again) # TODOSeventh, remove the invariant columns with c().
# call invar_omit() on bird_snps_num_t_again
bird_snps_no_invar <- c(bird_snps_num_t_again) #TODOCompare the original and the new dfs
# call dim() on bird_snps_num_t_again
dim(bird_snps_num_t_again) #TODO## [1] 72 1929# call dim() on bird_snps_no_invar
dim(bird_snps_no_invar) #TODO## NULLThese data have a lot of NAs. If we just call na.omit()
on them, what happens? Call c() on the
bird_snps_num_t_again object, then check the
dimensions.
# Call na.omit() on bird_snps_num_t
## and assign the output to no_NAs
no_NAs <- na.omit(bird_snps_num_t_again) # TODO
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## [97297] 0 0 0 0 0 0 NA NA 0 0 0 0 NA 0 NA 0 NA 0 0 0 0 0 0 0
## [97321] NA 0 0 NA NA 0 0 0 0 0 0 0 0 0 0 NA 0 0 0 NA 0 0 0 0
## [97345] 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0
## [97369] 0 0 0 0 0 0 0 0 0 0 0 0 NA 0 NA 0 NA 0 NA 0 NA 0 0 0
## [97393] 0 0 0 0 NA 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
## [97417] 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
## [97441] 0 0 0 0 0 0 0 0 0 0 0 0 NA 0 NA 0 NA 0 0 0 0 0 0 0
## [97465] 0 NA 0 NA NA 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
## [97489] 1 1 1 0 1 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0
## [97513] 1 0 0 0 0 0 NA NA 0 0 0 0 NA NA NA 0 NA 0 0 0 0 0 0 0
## [97537] NA 0 0 NA NA NA 0 NA 0 0 0 0 0 0 NA NA 0 NA 0 0 0 0 0 0
## [97561] 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
## [97585] 0 0 0 0 0 0 NA NA 0 0 0 0 NA NA NA 0 NA 0 0 0 0 0 1 1
## [97609] NA 1 1 NA NA NA 1 NA 1 1 0 1 1 1 NA NA 0 NA 1 1 1 1 0 1
## [97633] 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
## [97657] 0 0 0 0 0 0 NA NA 0 0 0 0 NA NA NA 0 NA 0 0 0 0 0 1 1
## [97681] NA 1 1 NA NA NA 1 NA 1 1 0 1 1 1 NA NA 0 NA 1 1 1 1 0 1
## [97705] 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
## [97729] 0 0 0 0 0 0 NA NA 0 0 0 0 NA NA NA 0 NA 0 0 0 0 0 0 0
## [97753] NA 0 0 NA NA NA 0 NA 0 0 0 0 0 0 NA NA 0 NA 0 0 0 0 0 0
## [97777] 1 1 0 0 0 0 0 1 0 0 0 1 0 0 1 0 1 0 1 0 0 0 1 0
## [97801] 0 0 0 0 0 0 NA 0 0 0 0 0 NA 0 NA 0 NA 0 NA 0 0 0 0 0
## [97825] 0 0 0 NA NA 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
## [97849] 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
## [97873] 0 0 0 0 0 0 NA 0 0 0 0 0 NA 0 NA 0 NA 0 NA 0 0 0 0 0
## [97897] 0 0 0 NA NA 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
## [97921] 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
## [97945] 0 0 0 0 0 0 NA 0 0 0 0 0 NA 0 NA 0 NA 0 NA 0 0 0 0 0
## [97969] 0 0 0 NA NA 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
## [97993] 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
## [98017] 0 0 0 0 0 0 0 0 0 0 0 NA NA 0 NA 0 NA 0 NA 0 0 0 0 0
## [98041] 0 0 0 0 NA 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 NA 0 0 0
## [98065] 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
## [98089] 0 0 0 0 0 0 NA 0 0 0 0 NA NA 0 NA 0 NA 0 NA 0 0 0 0 0
## [98113] 0 NA 0 NA NA 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
## [98137] 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
## [98161] 0 0 0 0 0 0 NA NA 0 0 NA 0 NA NA NA 0 NA 0 0 0 1 1 1 1
## [98185] NA NA 1 1 NA 1 0 1 0 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1
## [98209] 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
## [98233] 0 0 0 0 0 0 0 0 0 0 NA 0 NA NA NA 0 NA 0 NA 0 NA NA 0 0
## [98257] 0 NA 0 NA NA 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 NA NA 0
## [98281] 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
## [98305] 0 0 0 0 0 0 NA NA 0 0 NA 0 NA 0 NA 0 NA 0 NA 0 NA 0 0 0
## [98329] NA 0 0 NA NA 0 0 NA 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
## [98353] 1 1 0 1 1 0 0 0 1 1 0 0 1 0 1 0 1 1 0 0 1 0 1 0
## [98377] 0 0 0 0 0 0 NA NA 1 1 NA 1 NA 1 NA 1 NA 1 NA 1 NA 1 0 1
## [98401] NA 0 0 NA NA 0 1 NA 1 0 0 0 0 0 0 1 0 1 1 0 0 1 1 0
## [98425] 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
## [98449] 0 0 0 0 0 0 NA NA 0 0 NA 0 NA 0 NA 0 NA 0 NA 0 NA 0 1 0
## [98473] NA 0 0 NA NA 0 0 NA 0 0 0 0 0 1 1 1 0 0 1 0 1 0 0 1
## [98497] 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
## [98521] 0 0 0 0 0 0 NA 0 0 0 0 0 NA NA NA 0 NA 0 NA 0 NA NA 0 0
## [98545] 0 NA 0 0 NA 0 0 NA 0 NA NA 0 NA 0 0 0 0 0 0 NA 0 0 0 0
## [98569] 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
## [98593] 0 0 0 0 0 0 NA 0 0 0 0 0 NA NA NA 0 NA 0 NA 0 NA NA 0 0
## [98617] 0 NA 0 0 NA 0 0 NA 0 NA NA 0 NA 0 0 0 0 0 0 NA 0 0 0 0
## [98641] 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
## [98665] 0 0 0 0 0 1 NA 0 0 0 0 0 NA NA NA 0 NA 0 NA 0 NA NA 0 0
## [98689] 0 NA 0 0 NA 0 0 NA 0 NA NA 0 NA 0 0 0 0 0 0 NA 0 0 0 0
## [98713] 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
## [98737] 0 0 0 0 0 0 0 0 0 0 NA 0 NA NA NA 0 NA 0 0 NA 0 0 0 0
## [98761] 0 NA 0 NA NA NA 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
## [98785] 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
## [98809] 0 0 0 0 0 0 NA 0 0 0 0 0 NA NA NA 0 NA 0 0 0 0 0 0 0
## [98833] 0 0 0 NA NA 0 0 NA 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
## [98857] 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 1 1 0 0 1 0 0 0
## [98881] 0 0 0 0 0 0 0 0 0 0 0 0 NA 0 NA 0 NA 0 0 0 0 0 0 0
## [98905] 0 0 0 0 NA 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
## [98929] 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
## [98953] 0 0 0 0 0 0 0 0 0 0 0 0 NA 0 NA 0 NA 0 0 0 0 0 0 0
## [98977] 0 0 0 0 NA 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
## [99001] 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
## [99025] 0 0 0 0 0 0 0 0 0 0 0 0 0 0 NA 0 NA 0 0 0 0 0 0 0
## [99049] 0 0 0 0 NA 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
## [99073] 0 1 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
## [99097] 0 0 0 0 0 0 0 0 0 0 0 0 0 0 NA 0 NA 0 0 1 1 1 1 1
## [99121] 1 1 1 1 NA 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1
## [99145] 0 1 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
## [99169] 0 0 0 0 0 0 0 0 0 0 0 0 0 0 NA 0 NA 0 0 1 1 1 1 1
## [99193] 1 1 1 1 NA 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1
## [99217] 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0
## [99241] 0 0 0 0 0 0 NA NA 0 0 NA 0 NA 0 NA 0 NA 0 NA 0 0 0 0 0
## [99265] NA NA 0 NA NA NA 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
## [99289] 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
## [99313] 0 0 0 0 0 0 NA 0 0 0 0 0 0 0 NA 0 NA 0 0 0 0 0 0 0
## [99337] 0 0 1 0 NA 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
## [99361] 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
## [99385] 0 0 0 0 0 0 NA 0 0 0 0 0 NA NA NA 0 NA 0 0 0 NA 0 0 0
## [99409] 0 0 0 0 NA 0 0 0 0 0 0 0 NA 0 0 NA 0 0 0 NA 0 NA 0 0
## [99433] 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
## [99457] 0 0 0 0 0 0 NA 0 0 0 0 0 NA NA NA 0 NA 0 0 0 NA 1 1 1
## [99481] 1 1 1 1 NA 1 1 1 1 1 1 1 NA 1 1 NA 1 1 1 NA 1 NA 1 1
## [99505] 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
## [99529] 0 0 0 0 0 0 NA 0 0 0 0 0 NA NA NA 0 NA 0 0 0 NA 1 1 1
## [99553] 1 1 0 1 NA 1 0 0 1 1 1 1 NA 0 1 NA 1 0 1 NA 1 NA 1 1
## [99577] 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
## [99601] 0 0 0 0 0 0 NA 0 0 0 0 0 NA NA NA 0 NA 0 0 0 NA 0 0 0
## [99625] 0 0 0 0 NA 0 0 0 0 0 0 0 NA 0 0 NA 0 0 0 NA 0 NA 0 0
## [99649] 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
## [99673] 0 0 0 0 0 0 0 0 0 0 0 0 0 0 NA 0 0 0 0 0 0 0 0 0
## [99697] 0 0 0 0 NA 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
## [99721] 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
## [99745] 0 0 0 0 0 0 0 0 0 0 NA NA 0 NA NA 0 NA 0 NA 0 0 0 0 0
## [99769] 0 0 0 NA NA 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
## [99793] 0 0 0 0 0 NA 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
## [99817] 0 0 0 0 0 0 0 0 0 0 0 0 NA 0 NA 0 NA 0 0 0 1 1 1 1
## [99841] 1 NA 1 NA NA 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1
## [99865] 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
## [99889] 0 0 0 0 0 0 NA 0 0 0 0 NA NA 0 NA 0 NA 0 NA 0 0 NA 0 0
## [99913] NA NA 0 NA NA 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 NA 0 0
## [99937] 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
## [99961] 0 0 0 0 0 0 NA 0 0 0 0 NA NA 0 NA 0 NA 0 NA 0 0 NA 0 0
## [99985] NA NA 0 NA NA 0 0 0 0 0 0 0 0 0 0
## [ reached getOption("max.print") -- omitted 38889 entries ]# what is the remaining size of the data?
# why
dim(no_NAs)## [1] 0 1929