Diseño Factorial simple

area = área de daño foliar

set.seed(22)
area = c(rnorm(18, 3, 0.8), 
         rnorm(6, 0.2, 0.05))
inoculo = gl(4, 6, 24, paste0('D', 0:3))
dt = data.frame(area = round(sort(area), 3), 
                inoculo)
head(dt)
##    area inoculo
## 1 0.119      D0
## 2 0.148      D0
## 3 0.166      D0
## 4 0.171      D0
## 5 0.200      D0
## 6 0.247      D0

Comparación de medias

boxplot(dt$area~dt$inoculo)

Hipótesis

\[H_0: \mu_{D0} = \mu_{D1}= \cdots = \mu_{D3}\]

mod_1 = aov(dt$area~dt$inoculo)
tb = summary(mod_1)
ifelse(unlist(tb)[9] < 0.05, 'Rechazo Ho',
       'No rechazo Ho')
##      Pr(>F)1 
## "Rechazo Ho"
##      Pr(>F)1 
## "Rechazo Ho"

Prueba de comparaciones de Turkey

TukeyHSD(x = mod_1)
##   Tukey multiple comparisons of means
##     95% family-wise confidence level
## 
## Fit: aov(formula = dt$area ~ dt$inoculo)
## 
## $`dt$inoculo`
##            diff         lwr      upr     p adj
## D1-D0 2.3715000  1.84876038 2.894240 0.0000000
## D2-D0 2.8731667  2.35042705 3.395906 0.0000000
## D3-D0 4.0190000  3.49626038 4.541740 0.0000000
## D2-D1 0.5016667 -0.02107295 1.024406 0.0628165
## D3-D1 1.6475000  1.12476038 2.170240 0.0000001
## D3-D2 1.1458333  0.62309372 1.668573 0.0000299

Modelo del diseño

\[y_{ij} = \mu + \tau_i + \epsilon_{ij} i=1,2,3,4~(inoculo) j=1,2,\cdots,6~(repeticiones)\]

Revisión de supuestos Normalidad \[[H_0:~los~residuales~del~modelo~tienen~distribución~normal\]

res = mod_1$residuals
n = shapiro.test(res)
n
## 
##  Shapiro-Wilk normality test
## 
## data:  res
## W = 0.97101, p-value = 0.692
#No rechazo Ho, los residuales se distribuyen normalmente pvalor>0.05

Homoceasticidad de varianza \[var~_{D_0} = var~_{D_1} = \cdots = var~_{D_4}\]

bartlett.test(dt$area~dt$inoculo)
## 
##  Bartlett test of homogeneity of variances
## 
## data:  dt$area by dt$inoculo
## Bartlett's K-squared = 22.055, df = 3, p-value = 6.355e-05
#Rechazo Ho: varianzas por grupo son distintas, No se cumple el supuesto de homocedasticidad. Pvalor < 0.05

ANALISIS DE VARIANZA CON VARIANZAS DESIGUALES (ANOVA DE WELCH)

mod_2 = oneway.test(dt$area~dt$inoculo)
mod_2
## 
##  One-way analysis of means (not assuming equal variances)
## 
## data:  dt$area and dt$inoculo
## F = 727.7, num df = 3.0000, denom df = 8.8951, p-value = 5.925e-11

ANALISIS DE VARIANZA NO PARAMETRICO (KRUSKAL WALLIS)

mod_3 = kruskal.test(dt$area~dt$inoculo)
mod_3
## 
##  Kruskal-Wallis rank sum test
## 
## data:  dt$area by dt$inoculo
## Kruskal-Wallis chi-squared = 21.6, df = 3, p-value = 7.9e-05

ANOVA PERMUTACIONAL

mod_4 = RVAideMemoire::perm.anova(dt$area~dt$inoculo, nperm = 5000)
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mod_4
## Permutation Analysis of Variance Table
## 
## Response: dt$area
## 5000 permutations
##            Sum Sq Df Mean Sq F value Pr(>F)    
## dt$inoculo 51.465  3 17.1552  163.94  2e-04 ***
## Residuals   2.093 20  0.1046                   
## ---
## Signif. codes:  0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1

ANOVA desbalanceado

dt_2 = dt
dt_2$area[15] = NA
library(car)
## Loading required package: carData
mod_5 = Anova(lm(dt_2$area~dt_2$inoculo), 
              type = 'II')
mod_5
## Anova Table (Type II tests)
## 
## Response: dt_2$area
##              Sum Sq Df F value    Pr(>F)    
## dt_2$inoculo 51.261  3  156.04 1.464e-13 ***
## Residuals     2.081 19                      
## ---
## Signif. codes:  0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1