Qiu_lab_work Part 1

Sample Data, Two Photon dendritics pine imaging

Lab Equipment - whole cell patch clamp electrophysiology and LSPS mapping

Electrophysiology - different modalities

Laser Scanning Photostimulation Combined with Glutamate Uncaging to map neural circuits

Sample LSPS ciruit mapping data, somatosensory cortex

Customized nanoliter volume injection

Tracer injection, histology

16CH Plexon system, and single unit recording for cortical circuit plasticity

Single unit recording for visual cortex critical period plasticity

This is most recent lab work, activated microglia surrounding amyloid plaques and dense core stained by Thio-S

Coded murine behavior analysis, Ethovision raw data with MATLAB ploting

Illustration of various behavior modality analysis

Cre-dependent AAV-ChETA was injected in the PFC, followed by TAM injection to induce activity-dependent cre (TRAP2 mice line). In BLA, PFC terminals were stimulated with blue light, which cleads to time locked action potentials. In addition, similar approaches were used for inhibitory opsins, where yellow light leads to silencing of activity or hyperpolarization.

Juvenile social isolation induced labeling of activated neurons throughout the brain.

USV is a measure of communications, used in autism mouse models, we used machine learning to categorize the call types and automatic analysis/plotting

One pending ABRC grant application work

graphic abstract of Aim 2 - synaptic physiology and LSPS circuit mapping

graphic abstract of Aim 3 - optogenetics and cocaine self administration

Measuring neuronal activity using GCaMP6 and fiber photometry

Multichannel in vivo recording, spikes, LFPs, phase locking

MATLAB coded analysis for sorted spikes

Fast scanning cyclic voltametry FSCV(https://en.wikipedia.org/wiki/Fast-scan_cyclic_voltammetry) was used to measure fast dopamine dynamics in brain slices. This can be useful to measure the evoked dopamine release in grafted DA neurons following gene therapy. Carbon fiber electrode was placed in dorsal striatum, with stimulating electrode close by. stimulation leads to DA relase. Typical redox potential of DA is shown below.

Measure dopamine levels in brain slices using dLight DA sensor

AAV-dLight (https://pubmed.ncbi.nlm.nih.gov/33087905/) was injected into nucleus accumbens, acute slices were made, electrode stimulation leads to dynamic changes of dopamine signals. Captured at 20fps.

Using an Intan board, we recorded mouse EEG and EMG, establishing a proof of principle for the sleep related studies. MATLAB FMAtools toolbox is used to characterize sleep state (wakeful, quite, REM, Non-REM, etc).

For a video, see (https://www.youtube.com/watch?v=GMrS99ddK4A). Gorgenous ‘twinkle’ of dendritics spines represent calcium changes (miniature events) at spines.

Recent single cell RNAseq work - illustration of workflow of the 10X Genomics pipeline

A and B. Illustration of LSPS mapping technique; C. LSPS applied to map local excitatory inputs onto L5 projection neurons. D. Sample responses of soma, synaptic/excitatory, and inhibitory responses. G, H. LSPS applied to map hippocampus circuits.

Neuronal morphology analysis, spines, dendrite, IHC staining

This is a published NCBI dataset, mild traumatic brain injury.

Our group published the first paper (https://pubmed.ncbi.nlm.nih.gov/22934102/) on single neuron RNAseq in brain slices using patch seq in 2012. This has been used in a few large dataset where neuronal morphology and ephys property are explored jointly.