20221214_ICP-MS_FW_DW_for_3tomatoes_IAA_Salt-fixed
This is a ICP-MS analysis result, DW and FW for the same batch of samples we performed on pareto-front components calculation for 3 the tomatoes experienced IAA and salt treatments on plate. Original data can be found in 20220406_RSA_M248M058LA1511_IAA_100mM_Salt experiment done on December 2021.Because Low and High IAA had similar effect on root system architecture, I only traced and sent high IAA for ICP-MS analysis.
getwd()## [1] "C:/Users/Julkowska Lab/Desktop/R codes by Maryam/20220311_3tomato_accessions_salt_IAA_FW_DW_ICP-MS_plus_tie1-1_salt_only"setwd("C:/Users/Julkowska Lab/Desktop/R codes by Maryam/20220311_3tomato_accessions_salt_IAA_FW_DW_ICP-MS_plus_tie1-1_salt_only/")
list.files(pattern = ".csv")## [1] "12-16-21RootandShoot-fresh-weight-of-M248M058LA1511fromIAAsaltRSAanalysis-hiIAA-only.csv"
## [2] "20220311_3accessions_IAA_salt_plate_DW_ICP-ms.csv"
## [3] "20220311_3accessions_IAA_salt_plate_DW_ICP-ms2.csv"
## [4] "20220311_tie1-1_M82_salt_plate_DW_ICP-ms.csv"ICP <- read.csv("20220311_3accessions_IAA_salt_plate_DW_ICP-ms2.csv")
colnames(ICP)[1]<-"All.ID"
colnames(ICP)[7]<-"K.con.mg.per.mg.dry.weight"
colnames(ICP)[8]<-"Na.con.mg.per.mg.dry.weight"
ICPlibrary(ggplot2)
library(ggpubr)
library(multcompView)
aov(Na.con.mg.per.mg.dry.weight ~ All.ID, data = ICP)## Call:
## aov(formula = Na.con.mg.per.mg.dry.weight ~ All.ID, data = ICP)
##
## Terms:
## All.ID Residuals
## Sum of Squares 51018.45 814.00
## Deg. of Freedom 23 86
##
## Residual standard error: 3.076543
## Estimated effects may be unbalancedOutput <- TukeyHSD(aov(Na.con.mg.per.mg.dry.weight ~ All.ID, data = ICP))
Output## Tukey multiple comparisons of means
## 95% family-wise confidence level
##
## Fit: aov(formula = Na.con.mg.per.mg.dry.weight ~ All.ID, data = ICP)
##
## $All.ID
## diff lwr upr
## LA1511-RO-C-0.1-LA1511-RO-C 0.243383464 -7.06892632 7.5556933
## LA1511-RO-S-LA1511-RO-C 33.545899267 25.10237122 41.9894273
## LA1511-RO-S-0.1-LA1511-RO-C 39.834014584 32.52170479 47.1463244
## LA1511-SH-C-LA1511-RO-C 0.710214769 -6.60209502 8.0225246
## LA1511-SH-C-0.1-LA1511-RO-C 0.670821580 -6.64148821 7.9831314
## LA1511-SH-S-LA1511-RO-C 42.443838770 34.00031072 50.8873668
## LA1511-SH-S-0.1-LA1511-RO-C 41.462728120 34.15041833 48.7750379
## M058-RO-C-LA1511-RO-C -0.012938510 -7.32524830 7.2993713
## M058-RO-C-0.1-LA1511-RO-C 1.059914315 -6.25239547 8.3722241
## M058-RO-S-LA1511-RO-C 22.739928785 14.98405303 30.4958045
## M058-RO-S-0.1-LA1511-RO-C 28.175944436 20.86363465 35.4882542
## M058-SH-C-LA1511-RO-C 0.725245384 -6.58706441 8.0375552
## M058-SH-C-0.1-LA1511-RO-C 0.890437436 -6.42187235 8.2027472
## M058-SH-S-LA1511-RO-C 46.280772150 38.52489639 54.0366479
## M058-SH-S-0.1-LA1511-RO-C 52.429012784 45.11670299 59.7413226
## M248-RO-C-LA1511-RO-C 0.251564280 -7.06074551 7.5638741
## M248-RO-C-0.1-LA1511-RO-C 0.950524841 -6.36178495 8.2628346
## M248-RO-S-LA1511-RO-C 32.738038248 24.98216249 40.4939140
## M248-RO-S-0.1-LA1511-RO-C 39.560331443 31.80445569 47.3162072
## M248-SH-C-LA1511-RO-C 0.768806467 -6.54350332 8.0811163
## M248-SH-C-0.1-LA1511-RO-C 1.220211671 -6.09209812 8.5325215
## M248-SH-S-LA1511-RO-C 53.850344195 46.09446844 61.6062200
## M248-SH-S-0.1-LA1511-RO-C 62.088235860 54.33236010 69.8441116
## LA1511-RO-S-LA1511-RO-C-0.1 33.302515802 24.85898775 41.7460439
## LA1511-RO-S-0.1-LA1511-RO-C-0.1 39.590631120 32.27832133 46.9029409
## LA1511-SH-C-LA1511-RO-C-0.1 0.466831304 -6.84547849 7.7791411
## LA1511-SH-C-0.1-LA1511-RO-C-0.1 0.427438115 -6.88487167 7.7397479
## LA1511-SH-S-LA1511-RO-C-0.1 42.200455306 33.75692726 50.6439834
## LA1511-SH-S-0.1-LA1511-RO-C-0.1 41.219344656 33.90703487 48.5316544
## M058-RO-C-LA1511-RO-C-0.1 -0.256321974 -7.56863176 7.0559878
## M058-RO-C-0.1-LA1511-RO-C-0.1 0.816530850 -6.49577894 8.1288406
## M058-RO-S-LA1511-RO-C-0.1 22.496545321 14.74066956 30.2524211
## M058-RO-S-0.1-LA1511-RO-C-0.1 27.932560972 20.62025118 35.2448708
## M058-SH-C-LA1511-RO-C-0.1 0.481861920 -6.83044787 7.7941717
## M058-SH-C-0.1-LA1511-RO-C-0.1 0.647053972 -6.66525582 7.9593638
## M058-SH-S-LA1511-RO-C-0.1 46.037388686 38.28151293 53.7932644
## M058-SH-S-0.1-LA1511-RO-C-0.1 52.185629320 44.87331953 59.4979391
## M248-RO-C-LA1511-RO-C-0.1 0.008180816 -7.30412897 7.3204906
## M248-RO-C-0.1-LA1511-RO-C-0.1 0.707141377 -6.60516841 8.0194512
## M248-RO-S-LA1511-RO-C-0.1 32.494654783 24.73877903 40.2505305
## M248-RO-S-0.1-LA1511-RO-C-0.1 39.316947978 31.56107222 47.0728237
## M248-SH-C-LA1511-RO-C-0.1 0.525423003 -6.78688679 7.8377328
## M248-SH-C-0.1-LA1511-RO-C-0.1 0.976828207 -6.33548158 8.2891380
## M248-SH-S-LA1511-RO-C-0.1 53.606960731 45.85108497 61.3628365
## M248-SH-S-0.1-LA1511-RO-C-0.1 61.844852396 54.08897664 69.6007282
## LA1511-RO-S-0.1-LA1511-RO-S 6.288115317 -2.15541273 14.7316434
## LA1511-SH-C-LA1511-RO-S -32.835684498 -41.27921255 -24.3921564
## LA1511-SH-C-0.1-LA1511-RO-S -32.875077687 -41.31860574 -24.4315496
## LA1511-SH-S-LA1511-RO-S 8.897939503 -0.54221184 18.3380908
## LA1511-SH-S-0.1-LA1511-RO-S 7.916828853 -0.52669920 16.3603569
## M058-RO-C-LA1511-RO-S -33.558837777 -42.00236583 -25.1153097
## M058-RO-C-0.1-LA1511-RO-S -32.485984952 -40.92951300 -24.0424569
## M058-RO-S-LA1511-RO-S -10.805970482 -19.63642349 -1.9755175
## M058-RO-S-0.1-LA1511-RO-S -5.369954831 -13.81348288 3.0735732
## M058-SH-C-LA1511-RO-S -32.820653883 -41.26418193 -24.3771258
## M058-SH-C-0.1-LA1511-RO-S -32.655461831 -41.09898988 -24.2119338
## M058-SH-S-LA1511-RO-S 12.734872883 3.90441988 21.5653259
## M058-SH-S-0.1-LA1511-RO-S 18.883113517 10.43958547 27.3266416
## M248-RO-C-LA1511-RO-S -33.294334986 -41.73786304 -24.8508069
## M248-RO-C-0.1-LA1511-RO-S -32.595374425 -41.03890248 -24.1518464
## M248-RO-S-LA1511-RO-S -0.807861019 -9.63831402 8.0225920
## M248-RO-S-0.1-LA1511-RO-S 6.014432176 -2.81602083 14.8448852
## M248-SH-C-LA1511-RO-S -32.777092800 -41.22062085 -24.3335647
## M248-SH-C-0.1-LA1511-RO-S -32.325687596 -40.76921565 -23.8821595
## M248-SH-S-LA1511-RO-S 20.304444928 11.47399192 29.1348979
## M248-SH-S-0.1-LA1511-RO-S 28.542336593 19.71188359 37.3727896
## LA1511-SH-C-LA1511-RO-S-0.1 -39.123799816 -46.43610960 -31.8114900
## LA1511-SH-C-0.1-LA1511-RO-S-0.1 -39.163193004 -46.47550279 -31.8508832
## LA1511-SH-S-LA1511-RO-S-0.1 2.609824186 -5.83370386 11.0533522
## LA1511-SH-S-0.1-LA1511-RO-S-0.1 1.628713536 -5.68359625 8.9410233
## M058-RO-C-LA1511-RO-S-0.1 -39.846953094 -47.15926288 -32.5346433
## M058-RO-C-0.1-LA1511-RO-S-0.1 -38.774100270 -46.08641006 -31.4617905
## M058-RO-S-LA1511-RO-S-0.1 -17.094085799 -24.84996156 -9.3382100
## M058-RO-S-0.1-LA1511-RO-S-0.1 -11.658070148 -18.97037994 -4.3457604
## M058-SH-C-LA1511-RO-S-0.1 -39.108769200 -46.42107899 -31.7964594
## M058-SH-C-0.1-LA1511-RO-S-0.1 -38.943577148 -46.25588694 -31.6312674
## M058-SH-S-LA1511-RO-S-0.1 6.446757566 -1.30911819 14.2026333
## M058-SH-S-0.1-LA1511-RO-S-0.1 12.594998200 5.28268841 19.9073080
## M248-RO-C-LA1511-RO-S-0.1 -39.582450304 -46.89476009 -32.2701405
## M248-RO-C-0.1-LA1511-RO-S-0.1 -38.883489743 -46.19579953 -31.5711800
## M248-RO-S-LA1511-RO-S-0.1 -7.095976336 -14.85185209 0.6598994
## M248-RO-S-0.1-LA1511-RO-S-0.1 -0.273683142 -8.02955890 7.4821926
## M248-SH-C-LA1511-RO-S-0.1 -39.065208117 -46.37751791 -31.7528983
## M248-SH-C-0.1-LA1511-RO-S-0.1 -38.613802913 -45.92611270 -31.3014931
## M248-SH-S-LA1511-RO-S-0.1 14.016329611 6.26045385 21.7722054
## M248-SH-S-0.1-LA1511-RO-S-0.1 22.254221276 14.49834552 30.0100970
## LA1511-SH-C-0.1-LA1511-SH-C -0.039393189 -7.35170298 7.2729166
## LA1511-SH-S-LA1511-SH-C 41.733624002 33.29009595 50.1771521
## LA1511-SH-S-0.1-LA1511-SH-C 40.752513352 33.44020356 48.0648231
## M058-RO-C-LA1511-SH-C -0.723153278 -8.03546307 6.5891565
## M058-RO-C-0.1-LA1511-SH-C 0.349699546 -6.96261024 7.6620093
## M058-RO-S-LA1511-SH-C 22.029714017 14.27383826 29.7855898
## M058-RO-S-0.1-LA1511-SH-C 27.465729668 20.15341988 34.7780395
## M058-SH-C-LA1511-SH-C 0.015030615 -7.29727917 7.3273404
## M058-SH-C-0.1-LA1511-SH-C 0.180222667 -7.13208712 7.4925325
## M058-SH-S-LA1511-SH-C 45.570557382 37.81468162 53.3264331
## M058-SH-S-0.1-LA1511-SH-C 51.718798016 44.40648823 59.0311078
## M248-RO-C-LA1511-SH-C -0.458650488 -7.77096028 6.8536593
## M248-RO-C-0.1-LA1511-SH-C 0.240310073 -7.07199972 7.5526199
## M248-RO-S-LA1511-SH-C 32.027823479 24.27194772 39.7836992
## M248-RO-S-0.1-LA1511-SH-C 38.850116674 31.09424092 46.6059924
## M248-SH-C-LA1511-SH-C 0.058591699 -7.25371809 7.3709015
## M248-SH-C-0.1-LA1511-SH-C 0.509996902 -6.80231289 7.8223067
## M248-SH-S-LA1511-SH-C 53.140129427 45.38425367 60.8960052
## M248-SH-S-0.1-LA1511-SH-C 61.378021092 53.62214533 69.1338968
## LA1511-SH-S-LA1511-SH-C-0.1 41.773017190 33.32948914 50.2165452
## LA1511-SH-S-0.1-LA1511-SH-C-0.1 40.791906540 33.47959675 48.1042163
## M058-RO-C-LA1511-SH-C-0.1 -0.683760090 -7.99606988 6.6285497
## M058-RO-C-0.1-LA1511-SH-C-0.1 0.389092735 -6.92321705 7.7014025
## M058-RO-S-LA1511-SH-C-0.1 22.069107205 14.31323145 29.8249830
## M058-RO-S-0.1-LA1511-SH-C-0.1 27.505122856 20.19281307 34.8174326
## M058-SH-C-LA1511-SH-C-0.1 0.054423804 -7.25788599 7.3667336
## M058-SH-C-0.1-LA1511-SH-C-0.1 0.219615856 -7.09269393 7.5319256
## M058-SH-S-LA1511-SH-C-0.1 45.609950570 37.85407481 53.3658263
## M058-SH-S-0.1-LA1511-SH-C-0.1 51.758191204 44.44588142 59.0705010
## M248-RO-C-LA1511-SH-C-0.1 -0.419257299 -7.73156709 6.8930525
## M248-RO-C-0.1-LA1511-SH-C-0.1 0.279703262 -7.03260653 7.5920131
## M248-RO-S-LA1511-SH-C-0.1 32.067216668 24.31134091 39.8230924
## M248-RO-S-0.1-LA1511-SH-C-0.1 38.889509863 31.13363411 46.6453856
## M248-SH-C-LA1511-SH-C-0.1 0.097984887 -7.21432490 7.4102947
## M248-SH-C-0.1-LA1511-SH-C-0.1 0.549390091 -6.76291970 7.8616999
## M248-SH-S-LA1511-SH-C-0.1 53.179522615 45.42364686 60.9353984
## M248-SH-S-0.1-LA1511-SH-C-0.1 61.417414280 53.66153852 69.1732900
## LA1511-SH-S-0.1-LA1511-SH-S -0.981110650 -9.42463870 7.4624174
## M058-RO-C-LA1511-SH-S -42.456777280 -50.90030533 -34.0132492
## M058-RO-C-0.1-LA1511-SH-S -41.383924456 -49.82745251 -32.9403964
## M058-RO-S-LA1511-SH-S -19.703909985 -28.53436299 -10.8734570
## M058-RO-S-0.1-LA1511-SH-S -14.267894334 -22.71142238 -5.8243663
## M058-SH-C-LA1511-SH-S -41.718593386 -50.16212144 -33.2750653
## M058-SH-C-0.1-LA1511-SH-S -41.553401334 -49.99692938 -33.1098733
## M058-SH-S-LA1511-SH-S 3.836933380 -4.99351962 12.6673864
## M058-SH-S-0.1-LA1511-SH-S 9.985174014 1.54164596 18.4287021
## M248-RO-C-LA1511-SH-S -42.192274490 -50.63580254 -33.7487464
## M248-RO-C-0.1-LA1511-SH-S -41.493313929 -49.93684198 -33.0497859
## M248-RO-S-LA1511-SH-S -9.705800522 -18.53625353 -0.8753475
## M248-RO-S-0.1-LA1511-SH-S -2.883507328 -11.71396033 5.9469457
## M248-SH-C-LA1511-SH-S -41.675032303 -50.11856035 -33.2315043
## M248-SH-C-0.1-LA1511-SH-S -41.223627099 -49.66715515 -32.7800990
## M248-SH-S-LA1511-SH-S 11.406505425 2.57605242 20.2369584
## M248-SH-S-0.1-LA1511-SH-S 19.644397090 10.81394409 28.4748501
## M058-RO-C-LA1511-SH-S-0.1 -41.475666630 -48.78797642 -34.1633568
## M058-RO-C-0.1-LA1511-SH-S-0.1 -40.402813806 -47.71512359 -33.0905040
## M058-RO-S-LA1511-SH-S-0.1 -18.722799335 -26.47867509 -10.9669236
## M058-RO-S-0.1-LA1511-SH-S-0.1 -13.286783684 -20.59909347 -5.9744739
## M058-SH-C-LA1511-SH-S-0.1 -40.737482736 -48.04979253 -33.4251729
## M058-SH-C-0.1-LA1511-SH-S-0.1 -40.572290684 -47.88460047 -33.2599809
## M058-SH-S-LA1511-SH-S-0.1 4.818044030 -2.93783173 12.5739198
## M058-SH-S-0.1-LA1511-SH-S-0.1 10.966284664 3.65397487 18.2785945
## M248-RO-C-LA1511-SH-S-0.1 -41.211163840 -48.52347363 -33.8988541
## M248-RO-C-0.1-LA1511-SH-S-0.1 -40.512203279 -47.82451307 -33.1998935
## M248-RO-S-LA1511-SH-S-0.1 -8.724689873 -16.48056563 -0.9688141
## M248-RO-S-0.1-LA1511-SH-S-0.1 -1.902396678 -9.65827243 5.8534791
## M248-SH-C-LA1511-SH-S-0.1 -40.693921653 -48.00623144 -33.3816119
## M248-SH-C-0.1-LA1511-SH-S-0.1 -40.242516449 -47.55482624 -32.9302067
## M248-SH-S-LA1511-SH-S-0.1 12.387616075 4.63174032 20.1434918
## M248-SH-S-0.1-LA1511-SH-S-0.1 20.625507740 12.86963198 28.3813835
## M058-RO-C-0.1-M058-RO-C 1.072852824 -6.23945696 8.3851626
## M058-RO-S-M058-RO-C 22.752867295 14.99699154 30.5087431
## M058-RO-S-0.1-M058-RO-C 28.188882946 20.87657316 35.5011927
## M058-SH-C-M058-RO-C 0.738183894 -6.57412590 8.0504937
## M058-SH-C-0.1-M058-RO-C 0.903375946 -6.40893384 8.2156857
## M058-SH-S-M058-RO-C 46.293710660 38.53783490 54.0495864
## M058-SH-S-0.1-M058-RO-C 52.441951294 45.12964150 59.7542611
## M248-RO-C-M058-RO-C 0.264502790 -7.04780700 7.5768126
## M248-RO-C-0.1-M058-RO-C 0.963463351 -6.34884644 8.2757731
## M248-RO-S-M058-RO-C 32.750976757 24.99510100 40.5068525
## M248-RO-S-0.1-M058-RO-C 39.573269952 31.81739420 47.3291457
## M248-SH-C-M058-RO-C 0.781744977 -6.53056481 8.0940548
## M248-SH-C-0.1-M058-RO-C 1.233150181 -6.07915961 8.5454600
## M248-SH-S-M058-RO-C 53.863282705 46.10740695 61.6191585
## M248-SH-S-0.1-M058-RO-C 62.101174370 54.34529861 69.8570501
## M058-RO-S-M058-RO-C-0.1 21.680014471 13.92413871 29.4358902
## M058-RO-S-0.1-M058-RO-C-0.1 27.116030122 19.80372033 34.4283399
## M058-SH-C-M058-RO-C-0.1 -0.334668931 -7.64697872 6.9776409
## M058-SH-C-0.1-M058-RO-C-0.1 -0.169476879 -7.48178667 7.1428329
## M058-SH-S-M058-RO-C-0.1 45.220857836 37.46498208 52.9767336
## M058-SH-S-0.1-M058-RO-C-0.1 51.369098470 44.05678868 58.6814083
## M248-RO-C-M058-RO-C-0.1 -0.808350034 -8.12065982 6.5039598
## M248-RO-C-0.1-M058-RO-C-0.1 -0.109389473 -7.42169926 7.2029203
## M248-RO-S-M058-RO-C-0.1 31.678123933 23.92224818 39.4339997
## M248-RO-S-0.1-M058-RO-C-0.1 38.500417128 30.74454137 46.2562929
## M248-SH-C-M058-RO-C-0.1 -0.291107847 -7.60341764 7.0212019
## M248-SH-C-0.1-M058-RO-C-0.1 0.160297356 -7.15201243 7.4726071
## M248-SH-S-M058-RO-C-0.1 52.790429881 45.03455412 60.5463056
## M248-SH-S-0.1-M058-RO-C-0.1 61.028321546 53.27244579 68.7841973
## M058-RO-S-0.1-M058-RO-S 5.436015651 -2.31986011 13.1918914
## M058-SH-C-M058-RO-S -22.014683401 -29.77055916 -14.2588076
## M058-SH-C-0.1-M058-RO-S -21.849491349 -29.60536711 -14.0936156
## M058-SH-S-M058-RO-S 23.540843365 15.36543248 31.7162542
## M058-SH-S-0.1-M058-RO-S 29.689083999 21.93320824 37.4449598
## M248-RO-C-M058-RO-S -22.488364505 -30.24424026 -14.7324887
## M248-RO-C-0.1-M058-RO-S -21.789403944 -29.54527970 -14.0335282
## M248-RO-S-M058-RO-S 9.998109463 1.82269858 18.1735203
## M248-RO-S-0.1-M058-RO-S 16.820402657 8.64499178 24.9958135
## M248-SH-C-M058-RO-S -21.971122318 -29.72699808 -14.2152466
## M248-SH-C-0.1-M058-RO-S -21.519717114 -29.27559287 -13.7638414
## M248-SH-S-M058-RO-S 31.110415410 22.93500453 39.2858263
## M248-SH-S-0.1-M058-RO-S 39.348307075 31.17289619 47.5237180
## M058-SH-C-M058-RO-S-0.1 -27.450699052 -34.76300884 -20.1383893
## M058-SH-C-0.1-M058-RO-S-0.1 -27.285507000 -34.59781679 -19.9731972
## M058-SH-S-M058-RO-S-0.1 18.104827714 10.34895196 25.8607035
## M058-SH-S-0.1-M058-RO-S-0.1 24.253068348 16.94075856 31.5653781
## M248-RO-C-M058-RO-S-0.1 -27.924380156 -35.23668995 -20.6120704
## M248-RO-C-0.1-M058-RO-S-0.1 -27.225419595 -34.53772938 -19.9131098
## M248-RO-S-M058-RO-S-0.1 4.562093812 -3.19378195 12.3179696
## M248-RO-S-0.1-M058-RO-S-0.1 11.384387007 3.62851125 19.1402628
## M248-SH-C-M058-RO-S-0.1 -27.407137969 -34.71944776 -20.0948282
## M248-SH-C-0.1-M058-RO-S-0.1 -26.955732765 -34.26804255 -19.6434230
## M248-SH-S-M058-RO-S-0.1 25.674399759 17.91852400 33.4302755
## M248-SH-S-0.1-M058-RO-S-0.1 33.912291424 26.15641567 41.6681672
## M058-SH-C-0.1-M058-SH-C 0.165192052 -7.14711774 7.4775018
## M058-SH-S-M058-SH-C 45.555526766 37.79965101 53.3114025
## M058-SH-S-0.1-M058-SH-C 51.703767400 44.39145761 59.0160772
## M248-RO-C-M058-SH-C -0.473681104 -7.78599089 6.8386287
## M248-RO-C-0.1-M058-SH-C 0.225279457 -7.08703033 7.5375892
## M248-RO-S-M058-SH-C 32.012792864 24.25691711 39.7686686
## M248-RO-S-0.1-M058-SH-C 38.835086059 31.07921030 46.5909618
## M248-SH-C-M058-SH-C 0.043561083 -7.26874871 7.3558709
## M248-SH-C-0.1-M058-SH-C 0.494966287 -6.81734350 7.8072761
## M248-SH-S-M058-SH-C 53.125098811 45.36922305 60.8809746
## M248-SH-S-0.1-M058-SH-C 61.362990476 53.60711472 69.1188662
## M058-SH-S-M058-SH-C-0.1 45.390334714 37.63445896 53.1462105
## M058-SH-S-0.1-M058-SH-C-0.1 51.538575348 44.22626556 58.8508851
## M248-RO-C-M058-SH-C-0.1 -0.638873156 -7.95118294 6.6734366
## M248-RO-C-0.1-M058-SH-C-0.1 0.060087405 -7.25222238 7.3723972
## M248-RO-S-M058-SH-C-0.1 31.847600812 24.09172505 39.6034766
## M248-RO-S-0.1-M058-SH-C-0.1 38.669894007 30.91401825 46.4257698
## M248-SH-C-M058-SH-C-0.1 -0.121630969 -7.43394076 7.1906788
## M248-SH-C-0.1-M058-SH-C-0.1 0.329774235 -6.98253555 7.6420840
## M248-SH-S-M058-SH-C-0.1 52.959906759 45.20403100 60.7157825
## M248-SH-S-0.1-M058-SH-C-0.1 61.197798424 53.44192267 68.9536742
## M058-SH-S-0.1-M058-SH-S 6.148240634 -1.60763512 13.9041164
## M248-RO-C-M058-SH-S -46.029207870 -53.78508363 -38.2733321
## M248-RO-C-0.1-M058-SH-S -45.330247309 -53.08612307 -37.5743716
## M248-RO-S-M058-SH-S -13.542733902 -21.71814478 -5.3673230
## M248-RO-S-0.1-M058-SH-S -6.720440708 -14.89585159 1.4549702
## M248-SH-C-M058-SH-S -45.511965683 -53.26784144 -37.7560899
## M248-SH-C-0.1-M058-SH-S -45.060560479 -52.81643624 -37.3046847
## M248-SH-S-M058-SH-S 7.569572045 -0.60583884 15.7449829
## M248-SH-S-0.1-M058-SH-S 15.807463710 7.63205283 23.9828746
## M248-RO-C-M058-SH-S-0.1 -52.177448504 -59.48975829 -44.8651387
## M248-RO-C-0.1-M058-SH-S-0.1 -51.478487943 -58.79079773 -44.1661782
## M248-RO-S-M058-SH-S-0.1 -19.690974536 -27.44685029 -11.9350988
## M248-RO-S-0.1-M058-SH-S-0.1 -12.868681342 -20.62455710 -5.1128056
## M248-SH-C-M058-SH-S-0.1 -51.660206317 -58.97251611 -44.3478965
## M248-SH-C-0.1-M058-SH-S-0.1 -51.208801113 -58.52111090 -43.8964913
## M248-SH-S-M058-SH-S-0.1 1.421331411 -6.33454435 9.1772072
## M248-SH-S-0.1-M058-SH-S-0.1 9.659223076 1.90334732 17.4150988
## M248-RO-C-0.1-M248-RO-C 0.698960561 -6.61334923 8.0112704
## M248-RO-S-M248-RO-C 32.486473967 24.73059821 40.2423497
## M248-RO-S-0.1-M248-RO-C 39.308767162 31.55289141 47.0646429
## M248-SH-C-M248-RO-C 0.517242187 -6.79506760 7.8295520
## M248-SH-C-0.1-M248-RO-C 0.968647391 -6.34366240 8.2809572
## M248-SH-S-M248-RO-C 53.598779915 45.84290416 61.3546557
## M248-SH-S-0.1-M248-RO-C 61.836671580 54.08079582 69.5925473
## M248-RO-S-M248-RO-C-0.1 31.787513406 24.03163765 39.5433892
## M248-RO-S-0.1-M248-RO-C-0.1 38.609806601 30.85393084 46.3656824
## M248-SH-C-M248-RO-C-0.1 -0.181718374 -7.49402816 7.1305914
## M248-SH-C-0.1-M248-RO-C-0.1 0.269686830 -7.04262296 7.5819966
## M248-SH-S-M248-RO-C-0.1 52.899819354 45.14394360 60.6556951
## M248-SH-S-0.1-M248-RO-C-0.1 61.137711019 53.38183526 68.8935868
## M248-RO-S-0.1-M248-RO-S 6.822293195 -1.35311769 14.9977041
## M248-SH-C-M248-RO-S -31.969231781 -39.72510754 -24.2133560
## M248-SH-C-0.1-M248-RO-S -31.517826577 -39.27370233 -23.7619508
## M248-SH-S-M248-RO-S 21.112305948 12.93689507 29.2877168
## M248-SH-S-0.1-M248-RO-S 29.350197612 21.17478673 37.5256085
## M248-SH-C-M248-RO-S-0.1 -38.791524975 -46.54740073 -31.0356492
## M248-SH-C-0.1-M248-RO-S-0.1 -38.340119772 -46.09599553 -30.5842440
## M248-SH-S-M248-RO-S-0.1 14.290012753 6.11460187 22.4654236
## M248-SH-S-0.1-M248-RO-S-0.1 22.527904418 14.35249354 30.7033153
## M248-SH-C-0.1-M248-SH-C 0.451405204 -6.86090459 7.7637150
## M248-SH-S-M248-SH-C 53.081537728 45.32566197 60.8374135
## M248-SH-S-0.1-M248-SH-C 61.319429393 53.56355364 69.0753052
## M248-SH-S-M248-SH-C-0.1 52.630132524 44.87425677 60.3860083
## M248-SH-S-0.1-M248-SH-C-0.1 60.868024189 53.11214843 68.6238999
## M248-SH-S-0.1-M248-SH-S 8.237891665 0.06248078 16.4133025
## p adj
## LA1511-RO-C-0.1-LA1511-RO-C 1.0000000
## LA1511-RO-S-LA1511-RO-C 0.0000000
## LA1511-RO-S-0.1-LA1511-RO-C 0.0000000
## LA1511-SH-C-LA1511-RO-C 1.0000000
## LA1511-SH-C-0.1-LA1511-RO-C 1.0000000
## LA1511-SH-S-LA1511-RO-C 0.0000000
## LA1511-SH-S-0.1-LA1511-RO-C 0.0000000
## M058-RO-C-LA1511-RO-C 1.0000000
## M058-RO-C-0.1-LA1511-RO-C 1.0000000
## M058-RO-S-LA1511-RO-C 0.0000000
## M058-RO-S-0.1-LA1511-RO-C 0.0000000
## M058-SH-C-LA1511-RO-C 1.0000000
## M058-SH-C-0.1-LA1511-RO-C 1.0000000
## M058-SH-S-LA1511-RO-C 0.0000000
## M058-SH-S-0.1-LA1511-RO-C 0.0000000
## M248-RO-C-LA1511-RO-C 1.0000000
## M248-RO-C-0.1-LA1511-RO-C 1.0000000
## M248-RO-S-LA1511-RO-C 0.0000000
## M248-RO-S-0.1-LA1511-RO-C 0.0000000
## M248-SH-C-LA1511-RO-C 1.0000000
## M248-SH-C-0.1-LA1511-RO-C 1.0000000
## M248-SH-S-LA1511-RO-C 0.0000000
## M248-SH-S-0.1-LA1511-RO-C 0.0000000
## LA1511-RO-S-LA1511-RO-C-0.1 0.0000000
## LA1511-RO-S-0.1-LA1511-RO-C-0.1 0.0000000
## LA1511-SH-C-LA1511-RO-C-0.1 1.0000000
## LA1511-SH-C-0.1-LA1511-RO-C-0.1 1.0000000
## LA1511-SH-S-LA1511-RO-C-0.1 0.0000000
## LA1511-SH-S-0.1-LA1511-RO-C-0.1 0.0000000
## M058-RO-C-LA1511-RO-C-0.1 1.0000000
## M058-RO-C-0.1-LA1511-RO-C-0.1 1.0000000
## M058-RO-S-LA1511-RO-C-0.1 0.0000000
## M058-RO-S-0.1-LA1511-RO-C-0.1 0.0000000
## M058-SH-C-LA1511-RO-C-0.1 1.0000000
## M058-SH-C-0.1-LA1511-RO-C-0.1 1.0000000
## M058-SH-S-LA1511-RO-C-0.1 0.0000000
## M058-SH-S-0.1-LA1511-RO-C-0.1 0.0000000
## M248-RO-C-LA1511-RO-C-0.1 1.0000000
## M248-RO-C-0.1-LA1511-RO-C-0.1 1.0000000
## M248-RO-S-LA1511-RO-C-0.1 0.0000000
## M248-RO-S-0.1-LA1511-RO-C-0.1 0.0000000
## M248-SH-C-LA1511-RO-C-0.1 1.0000000
## M248-SH-C-0.1-LA1511-RO-C-0.1 1.0000000
## M248-SH-S-LA1511-RO-C-0.1 0.0000000
## M248-SH-S-0.1-LA1511-RO-C-0.1 0.0000000
## LA1511-RO-S-0.1-LA1511-RO-S 0.4526375
## LA1511-SH-C-LA1511-RO-S 0.0000000
## LA1511-SH-C-0.1-LA1511-RO-S 0.0000000
## LA1511-SH-S-LA1511-RO-S 0.0912384
## LA1511-SH-S-0.1-LA1511-RO-S 0.0958436
## M058-RO-C-LA1511-RO-S 0.0000000
## M058-RO-C-0.1-LA1511-RO-S 0.0000000
## M058-RO-S-LA1511-RO-S 0.0031480
## M058-RO-S-0.1-LA1511-RO-S 0.7485573
## M058-SH-C-LA1511-RO-S 0.0000000
## M058-SH-C-0.1-LA1511-RO-S 0.0000000
## M058-SH-S-LA1511-RO-S 0.0001328
## M058-SH-S-0.1-LA1511-RO-S 0.0000000
## M248-RO-C-LA1511-RO-S 0.0000000
## M248-RO-C-0.1-LA1511-RO-S 0.0000000
## M248-RO-S-LA1511-RO-S 1.0000000
## M248-RO-S-0.1-LA1511-RO-S 0.6292815
## M248-SH-C-LA1511-RO-S 0.0000000
## M248-SH-C-0.1-LA1511-RO-S 0.0000000
## M248-SH-S-LA1511-RO-S 0.0000000
## M248-SH-S-0.1-LA1511-RO-S 0.0000000
## LA1511-SH-C-LA1511-RO-S-0.1 0.0000000
## LA1511-SH-C-0.1-LA1511-RO-S-0.1 0.0000000
## LA1511-SH-S-LA1511-RO-S-0.1 0.9999590
## LA1511-SH-S-0.1-LA1511-RO-S-0.1 0.9999999
## M058-RO-C-LA1511-RO-S-0.1 0.0000000
## M058-RO-C-0.1-LA1511-RO-S-0.1 0.0000000
## M058-RO-S-LA1511-RO-S-0.1 0.0000000
## M058-RO-S-0.1-LA1511-RO-S-0.1 0.0000122
## M058-SH-C-LA1511-RO-S-0.1 0.0000000
## M058-SH-C-0.1-LA1511-RO-S-0.1 0.0000000
## M058-SH-S-LA1511-RO-S-0.1 0.2482765
## M058-SH-S-0.1-LA1511-RO-S-0.1 0.0000015
## M248-RO-C-LA1511-RO-S-0.1 0.0000000
## M248-RO-C-0.1-LA1511-RO-S-0.1 0.0000000
## M248-RO-S-LA1511-RO-S-0.1 0.1195240
## M248-RO-S-0.1-LA1511-RO-S-0.1 1.0000000
## M248-SH-C-LA1511-RO-S-0.1 0.0000000
## M248-SH-C-0.1-LA1511-RO-S-0.1 0.0000000
## M248-SH-S-LA1511-RO-S-0.1 0.0000004
## M248-SH-S-0.1-LA1511-RO-S-0.1 0.0000000
## LA1511-SH-C-0.1-LA1511-SH-C 1.0000000
## LA1511-SH-S-LA1511-SH-C 0.0000000
## LA1511-SH-S-0.1-LA1511-SH-C 0.0000000
## M058-RO-C-LA1511-SH-C 1.0000000
## M058-RO-C-0.1-LA1511-SH-C 1.0000000
## M058-RO-S-LA1511-SH-C 0.0000000
## M058-RO-S-0.1-LA1511-SH-C 0.0000000
## M058-SH-C-LA1511-SH-C 1.0000000
## M058-SH-C-0.1-LA1511-SH-C 1.0000000
## M058-SH-S-LA1511-SH-C 0.0000000
## M058-SH-S-0.1-LA1511-SH-C 0.0000000
## M248-RO-C-LA1511-SH-C 1.0000000
## M248-RO-C-0.1-LA1511-SH-C 1.0000000
## M248-RO-S-LA1511-SH-C 0.0000000
## M248-RO-S-0.1-LA1511-SH-C 0.0000000
## M248-SH-C-LA1511-SH-C 1.0000000
## M248-SH-C-0.1-LA1511-SH-C 1.0000000
## M248-SH-S-LA1511-SH-C 0.0000000
## M248-SH-S-0.1-LA1511-SH-C 0.0000000
## LA1511-SH-S-LA1511-SH-C-0.1 0.0000000
## LA1511-SH-S-0.1-LA1511-SH-C-0.1 0.0000000
## M058-RO-C-LA1511-SH-C-0.1 1.0000000
## M058-RO-C-0.1-LA1511-SH-C-0.1 1.0000000
## M058-RO-S-LA1511-SH-C-0.1 0.0000000
## M058-RO-S-0.1-LA1511-SH-C-0.1 0.0000000
## M058-SH-C-LA1511-SH-C-0.1 1.0000000
## M058-SH-C-0.1-LA1511-SH-C-0.1 1.0000000
## M058-SH-S-LA1511-SH-C-0.1 0.0000000
## M058-SH-S-0.1-LA1511-SH-C-0.1 0.0000000
## M248-RO-C-LA1511-SH-C-0.1 1.0000000
## M248-RO-C-0.1-LA1511-SH-C-0.1 1.0000000
## M248-RO-S-LA1511-SH-C-0.1 0.0000000
## M248-RO-S-0.1-LA1511-SH-C-0.1 0.0000000
## M248-SH-C-LA1511-SH-C-0.1 1.0000000
## M248-SH-C-0.1-LA1511-SH-C-0.1 1.0000000
## M248-SH-S-LA1511-SH-C-0.1 0.0000000
## M248-SH-S-0.1-LA1511-SH-C-0.1 0.0000000
## LA1511-SH-S-0.1-LA1511-SH-S 1.0000000
## M058-RO-C-LA1511-SH-S 0.0000000
## M058-RO-C-0.1-LA1511-SH-S 0.0000000
## M058-RO-S-LA1511-SH-S 0.0000000
## M058-RO-S-0.1-LA1511-SH-S 0.0000026
## M058-SH-C-LA1511-SH-S 0.0000000
## M058-SH-C-0.1-LA1511-SH-S 0.0000000
## M058-SH-S-LA1511-SH-S 0.9933239
## M058-SH-S-0.1-LA1511-SH-S 0.0054620
## M248-RO-C-LA1511-SH-S 0.0000000
## M248-RO-C-0.1-LA1511-SH-S 0.0000000
## M248-RO-S-LA1511-SH-S 0.0158143
## M248-RO-S-0.1-LA1511-SH-S 0.9998975
## M248-SH-C-LA1511-SH-S 0.0000000
## M248-SH-C-0.1-LA1511-SH-S 0.0000000
## M248-SH-S-LA1511-SH-S 0.0012229
## M248-SH-S-0.1-LA1511-SH-S 0.0000000
## M058-RO-C-LA1511-SH-S-0.1 0.0000000
## M058-RO-C-0.1-LA1511-SH-S-0.1 0.0000000
## M058-RO-S-LA1511-SH-S-0.1 0.0000000
## M058-RO-S-0.1-LA1511-SH-S-0.1 0.0000003
## M058-SH-C-LA1511-SH-S-0.1 0.0000000
## M058-SH-C-0.1-LA1511-SH-S-0.1 0.0000000
## M058-SH-S-LA1511-SH-S-0.1 0.7839460
## M058-SH-S-0.1-LA1511-SH-S-0.1 0.0000546
## M248-RO-C-LA1511-SH-S-0.1 0.0000000
## M248-RO-C-0.1-LA1511-SH-S-0.1 0.0000000
## M248-RO-S-LA1511-SH-S-0.1 0.0114827
## M248-RO-S-0.1-LA1511-SH-S-0.1 0.9999994
## M248-SH-C-LA1511-SH-S-0.1 0.0000000
## M248-SH-C-0.1-LA1511-SH-S-0.1 0.0000000
## M248-SH-S-LA1511-SH-S-0.1 0.0000116
## M248-SH-S-0.1-LA1511-SH-S-0.1 0.0000000
## M058-RO-C-0.1-M058-RO-C 1.0000000
## M058-RO-S-M058-RO-C 0.0000000
## M058-RO-S-0.1-M058-RO-C 0.0000000
## M058-SH-C-M058-RO-C 1.0000000
## M058-SH-C-0.1-M058-RO-C 1.0000000
## M058-SH-S-M058-RO-C 0.0000000
## M058-SH-S-0.1-M058-RO-C 0.0000000
## M248-RO-C-M058-RO-C 1.0000000
## M248-RO-C-0.1-M058-RO-C 1.0000000
## M248-RO-S-M058-RO-C 0.0000000
## M248-RO-S-0.1-M058-RO-C 0.0000000
## M248-SH-C-M058-RO-C 1.0000000
## M248-SH-C-0.1-M058-RO-C 1.0000000
## M248-SH-S-M058-RO-C 0.0000000
## M248-SH-S-0.1-M058-RO-C 0.0000000
## M058-RO-S-M058-RO-C-0.1 0.0000000
## M058-RO-S-0.1-M058-RO-C-0.1 0.0000000
## M058-SH-C-M058-RO-C-0.1 1.0000000
## M058-SH-C-0.1-M058-RO-C-0.1 1.0000000
## M058-SH-S-M058-RO-C-0.1 0.0000000
## M058-SH-S-0.1-M058-RO-C-0.1 0.0000000
## M248-RO-C-M058-RO-C-0.1 1.0000000
## M248-RO-C-0.1-M058-RO-C-0.1 1.0000000
## M248-RO-S-M058-RO-C-0.1 0.0000000
## M248-RO-S-0.1-M058-RO-C-0.1 0.0000000
## M248-SH-C-M058-RO-C-0.1 1.0000000
## M248-SH-C-0.1-M058-RO-C-0.1 1.0000000
## M248-SH-S-M058-RO-C-0.1 0.0000000
## M248-SH-S-0.1-M058-RO-C-0.1 0.0000000
## M058-RO-S-0.1-M058-RO-S 0.5740450
## M058-SH-C-M058-RO-S 0.0000000
## M058-SH-C-0.1-M058-RO-S 0.0000000
## M058-SH-S-M058-RO-S 0.0000000
## M058-SH-S-0.1-M058-RO-S 0.0000000
## M248-RO-C-M058-RO-S 0.0000000
## M248-RO-C-0.1-M058-RO-S 0.0000000
## M248-RO-S-M058-RO-S 0.0031810
## M248-RO-S-0.1-M058-RO-S 0.0000000
## M248-SH-C-M058-RO-S 0.0000000
## M248-SH-C-0.1-M058-RO-S 0.0000000
## M248-SH-S-M058-RO-S 0.0000000
## M248-SH-S-0.1-M058-RO-S 0.0000000
## M058-SH-C-M058-RO-S-0.1 0.0000000
## M058-SH-C-0.1-M058-RO-S-0.1 0.0000000
## M058-SH-S-M058-RO-S-0.1 0.0000000
## M058-SH-S-0.1-M058-RO-S-0.1 0.0000000
## M248-RO-C-M058-RO-S-0.1 0.0000000
## M248-RO-C-0.1-M058-RO-S-0.1 0.0000000
## M248-RO-S-M058-RO-S-0.1 0.8536407
## M248-RO-S-0.1-M058-RO-S-0.1 0.0000895
## M248-SH-C-M058-RO-S-0.1 0.0000000
## M248-SH-C-0.1-M058-RO-S-0.1 0.0000000
## M248-SH-S-M058-RO-S-0.1 0.0000000
## M248-SH-S-0.1-M058-RO-S-0.1 0.0000000
## M058-SH-C-0.1-M058-SH-C 1.0000000
## M058-SH-S-M058-SH-C 0.0000000
## M058-SH-S-0.1-M058-SH-C 0.0000000
## M248-RO-C-M058-SH-C 1.0000000
## M248-RO-C-0.1-M058-SH-C 1.0000000
## M248-RO-S-M058-SH-C 0.0000000
## M248-RO-S-0.1-M058-SH-C 0.0000000
## M248-SH-C-M058-SH-C 1.0000000
## M248-SH-C-0.1-M058-SH-C 1.0000000
## M248-SH-S-M058-SH-C 0.0000000
## M248-SH-S-0.1-M058-SH-C 0.0000000
## M058-SH-S-M058-SH-C-0.1 0.0000000
## M058-SH-S-0.1-M058-SH-C-0.1 0.0000000
## M248-RO-C-M058-SH-C-0.1 1.0000000
## M248-RO-C-0.1-M058-SH-C-0.1 1.0000000
## M248-RO-S-M058-SH-C-0.1 0.0000000
## M248-RO-S-0.1-M058-SH-C-0.1 0.0000000
## M248-SH-C-M058-SH-C-0.1 1.0000000
## M248-SH-C-0.1-M058-SH-C-0.1 1.0000000
## M248-SH-S-M058-SH-C-0.1 0.0000000
## M248-SH-S-0.1-M058-SH-C-0.1 0.0000000
## M058-SH-S-0.1-M058-SH-S 0.3311554
## M248-RO-C-M058-SH-S 0.0000000
## M248-RO-C-0.1-M058-SH-S 0.0000000
## M248-RO-S-M058-SH-S 0.0000044
## M248-RO-S-0.1-M058-SH-S 0.2667040
## M248-SH-C-M058-SH-S 0.0000000
## M248-SH-C-0.1-M058-SH-S 0.0000000
## M248-SH-S-M058-SH-S 0.1075413
## M248-SH-S-0.1-M058-SH-S 0.0000000
## M248-RO-C-M058-SH-S-0.1 0.0000000
## M248-RO-C-0.1-M058-SH-S-0.1 0.0000000
## M248-RO-S-M058-SH-S-0.1 0.0000000
## M248-RO-S-0.1-M058-SH-S-0.1 0.0000043
## M248-SH-C-M058-SH-S-0.1 0.0000000
## M248-SH-C-0.1-M058-SH-S-0.1 0.0000000
## M248-SH-S-M058-SH-S-0.1 1.0000000
## M248-SH-S-0.1-M058-SH-S-0.1 0.0023388
## M248-RO-C-0.1-M248-RO-C 1.0000000
## M248-RO-S-M248-RO-C 0.0000000
## M248-RO-S-0.1-M248-RO-C 0.0000000
## M248-SH-C-M248-RO-C 1.0000000
## M248-SH-C-0.1-M248-RO-C 1.0000000
## M248-SH-S-M248-RO-C 0.0000000
## M248-SH-S-0.1-M248-RO-C 0.0000000
## M248-RO-S-M248-RO-C-0.1 0.0000000
## M248-RO-S-0.1-M248-RO-C-0.1 0.0000000
## M248-SH-C-M248-RO-C-0.1 1.0000000
## M248-SH-C-0.1-M248-RO-C-0.1 1.0000000
## M248-SH-S-M248-RO-C-0.1 0.0000000
## M248-SH-S-0.1-M248-RO-C-0.1 0.0000000
## M248-RO-S-0.1-M248-RO-S 0.2419008
## M248-SH-C-M248-RO-S 0.0000000
## M248-SH-C-0.1-M248-RO-S 0.0000000
## M248-SH-S-M248-RO-S 0.0000000
## M248-SH-S-0.1-M248-RO-S 0.0000000
## M248-SH-C-M248-RO-S-0.1 0.0000000
## M248-SH-C-0.1-M248-RO-S-0.1 0.0000000
## M248-SH-S-M248-RO-S-0.1 0.0000010
## M248-SH-S-0.1-M248-RO-S-0.1 0.0000000
## M248-SH-C-0.1-M248-SH-C 1.0000000
## M248-SH-S-M248-SH-C 0.0000000
## M248-SH-S-0.1-M248-SH-C 0.0000000
## M248-SH-S-M248-SH-C-0.1 0.0000000
## M248-SH-S-0.1-M248-SH-C-0.1 0.0000000
## M248-SH-S-0.1-M248-SH-S 0.0459768#P8 = Output$All.ID[,'p adj']
#stat.test<- multcompLetters(P8)
#stat.test
#I was trying to plot Na content for All.ID......but got error....so I try to separate SH and RO
colnames(ICP)## [1] "All.ID" "Accession"
## [3] "Condition" "Tissue"
## [5] "DW.g" "DW.mg"
## [7] "K.con.mg.per.mg.dry.weight" "Na.con.mg.per.mg.dry.weight"
## [9] "Na.k.ratio"ICP$GenoConTiss <- paste(ICP$Condition, ICP$Tissue, ICP$Accession, sep="_")
ICPaov(Na.con.mg.per.mg.dry.weight ~ GenoConTiss, data = ICP)## Call:
## aov(formula = Na.con.mg.per.mg.dry.weight ~ GenoConTiss, data = ICP)
##
## Terms:
## GenoConTiss Residuals
## Sum of Squares 51018.45 814.00
## Deg. of Freedom 23 86
##
## Residual standard error: 3.076543
## Estimated effects may be unbalancedOutput <- TukeyHSD(aov(Na.con.mg.per.mg.dry.weight ~ GenoConTiss, data = ICP))
Output## Tukey multiple comparisons of means
## 95% family-wise confidence level
##
## Fit: aov(formula = Na.con.mg.per.mg.dry.weight ~ GenoConTiss, data = ICP)
##
## $GenoConTiss
## diff lwr upr p adj
## C_RO_M058-C_RO_LA1511 -0.012938510 -7.32524830 7.299371 1.0000000
## C_RO_M248-C_RO_LA1511 0.251564280 -7.06074551 7.563874 1.0000000
## C_SH_LA1511-C_RO_LA1511 0.710214769 -6.60209502 8.022525 1.0000000
## C_SH_M058-C_RO_LA1511 0.725245384 -6.58706441 8.037555 1.0000000
## C_SH_M248-C_RO_LA1511 0.768806467 -6.54350332 8.081116 1.0000000
## C+IAA_RO_LA1511-C_RO_LA1511 0.243383464 -7.06892632 7.555693 1.0000000
## C+IAA_RO_M058-C_RO_LA1511 1.059914315 -6.25239547 8.372224 1.0000000
## C+IAA_RO_M248-C_RO_LA1511 0.950524841 -6.36178495 8.262835 1.0000000
## C+IAA_SH_LA1511-C_RO_LA1511 0.670821580 -6.64148821 7.983131 1.0000000
## C+IAA_SH_M058-C_RO_LA1511 0.890437436 -6.42187235 8.202747 1.0000000
## C+IAA_SH_M248-C_RO_LA1511 1.220211671 -6.09209812 8.532521 1.0000000
## S_RO_LA1511-C_RO_LA1511 33.545899267 25.10237122 41.989427 0.0000000
## S_RO_M058-C_RO_LA1511 22.739928785 14.98405303 30.495805 0.0000000
## S_RO_M248-C_RO_LA1511 32.738038248 24.98216249 40.493914 0.0000000
## S_SH_LA1511-C_RO_LA1511 42.443838770 34.00031072 50.887367 0.0000000
## S_SH_M058-C_RO_LA1511 46.280772150 38.52489639 54.036648 0.0000000
## S_SH_M248-C_RO_LA1511 53.850344195 46.09446844 61.606220 0.0000000
## S+IAA_RO_LA1511-C_RO_LA1511 39.834014584 32.52170479 47.146324 0.0000000
## S+IAA_RO_M058-C_RO_LA1511 28.175944436 20.86363465 35.488254 0.0000000
## S+IAA_RO_M248-C_RO_LA1511 39.560331443 31.80445569 47.316207 0.0000000
## S+IAA_SH_LA1511-C_RO_LA1511 41.462728120 34.15041833 48.775038 0.0000000
## S+IAA_SH_M058-C_RO_LA1511 52.429012784 45.11670299 59.741323 0.0000000
## S+IAA_SH_M248-C_RO_LA1511 62.088235860 54.33236010 69.844112 0.0000000
## C_RO_M248-C_RO_M058 0.264502790 -7.04780700 7.576813 1.0000000
## C_SH_LA1511-C_RO_M058 0.723153278 -6.58915651 8.035463 1.0000000
## C_SH_M058-C_RO_M058 0.738183894 -6.57412590 8.050494 1.0000000
## C_SH_M248-C_RO_M058 0.781744977 -6.53056481 8.094055 1.0000000
## C+IAA_RO_LA1511-C_RO_M058 0.256321974 -7.05598782 7.568632 1.0000000
## C+IAA_RO_M058-C_RO_M058 1.072852824 -6.23945696 8.385163 1.0000000
## C+IAA_RO_M248-C_RO_M058 0.963463351 -6.34884644 8.275773 1.0000000
## C+IAA_SH_LA1511-C_RO_M058 0.683760090 -6.62854970 7.996070 1.0000000
## C+IAA_SH_M058-C_RO_M058 0.903375946 -6.40893384 8.215686 1.0000000
## C+IAA_SH_M248-C_RO_M058 1.233150181 -6.07915961 8.545460 1.0000000
## S_RO_LA1511-C_RO_M058 33.558837777 25.11530973 42.002366 0.0000000
## S_RO_M058-C_RO_M058 22.752867295 14.99699154 30.508743 0.0000000
## S_RO_M248-C_RO_M058 32.750976757 24.99510100 40.506853 0.0000000
## S_SH_LA1511-C_RO_M058 42.456777280 34.01324923 50.900305 0.0000000
## S_SH_M058-C_RO_M058 46.293710660 38.53783490 54.049586 0.0000000
## S_SH_M248-C_RO_M058 53.863282705 46.10740695 61.619158 0.0000000
## S+IAA_RO_LA1511-C_RO_M058 39.846953094 32.53464330 47.159263 0.0000000
## S+IAA_RO_M058-C_RO_M058 28.188882946 20.87657316 35.501193 0.0000000
## S+IAA_RO_M248-C_RO_M058 39.573269952 31.81739420 47.329146 0.0000000
## S+IAA_SH_LA1511-C_RO_M058 41.475666630 34.16335684 48.787976 0.0000000
## S+IAA_SH_M058-C_RO_M058 52.441951294 45.12964150 59.754261 0.0000000
## S+IAA_SH_M248-C_RO_M058 62.101174370 54.34529861 69.857050 0.0000000
## C_SH_LA1511-C_RO_M248 0.458650488 -6.85365930 7.770960 1.0000000
## C_SH_M058-C_RO_M248 0.473681104 -6.83862869 7.785991 1.0000000
## C_SH_M248-C_RO_M248 0.517242187 -6.79506760 7.829552 1.0000000
## C+IAA_RO_LA1511-C_RO_M248 -0.008180816 -7.32049061 7.304129 1.0000000
## C+IAA_RO_M058-C_RO_M248 0.808350034 -6.50395976 8.120660 1.0000000
## C+IAA_RO_M248-C_RO_M248 0.698960561 -6.61334923 8.011270 1.0000000
## C+IAA_SH_LA1511-C_RO_M248 0.419257299 -6.89305249 7.731567 1.0000000
## C+IAA_SH_M058-C_RO_M248 0.638873156 -6.67343663 7.951183 1.0000000
## C+IAA_SH_M248-C_RO_M248 0.968647391 -6.34366240 8.280957 1.0000000
## S_RO_LA1511-C_RO_M248 33.294334986 24.85080694 41.737863 0.0000000
## S_RO_M058-C_RO_M248 22.488364505 14.73248875 30.244240 0.0000000
## S_RO_M248-C_RO_M248 32.486473967 24.73059821 40.242350 0.0000000
## S_SH_LA1511-C_RO_M248 42.192274490 33.74874644 50.635803 0.0000000
## S_SH_M058-C_RO_M248 46.029207870 38.27333211 53.785084 0.0000000
## S_SH_M248-C_RO_M248 53.598779915 45.84290416 61.354656 0.0000000
## S+IAA_RO_LA1511-C_RO_M248 39.582450304 32.27014051 46.894760 0.0000000
## S+IAA_RO_M058-C_RO_M248 27.924380156 20.61207037 35.236690 0.0000000
## S+IAA_RO_M248-C_RO_M248 39.308767162 31.55289141 47.064643 0.0000000
## S+IAA_SH_LA1511-C_RO_M248 41.211163840 33.89885405 48.523474 0.0000000
## S+IAA_SH_M058-C_RO_M248 52.177448504 44.86513871 59.489758 0.0000000
## S+IAA_SH_M248-C_RO_M248 61.836671580 54.08079582 69.592547 0.0000000
## C_SH_M058-C_SH_LA1511 0.015030615 -7.29727917 7.327340 1.0000000
## C_SH_M248-C_SH_LA1511 0.058591699 -7.25371809 7.370901 1.0000000
## C+IAA_RO_LA1511-C_SH_LA1511 -0.466831304 -7.77914109 6.845478 1.0000000
## C+IAA_RO_M058-C_SH_LA1511 0.349699546 -6.96261024 7.662009 1.0000000
## C+IAA_RO_M248-C_SH_LA1511 0.240310073 -7.07199972 7.552620 1.0000000
## C+IAA_SH_LA1511-C_SH_LA1511 -0.039393189 -7.35170298 7.272917 1.0000000
## C+IAA_SH_M058-C_SH_LA1511 0.180222667 -7.13208712 7.492532 1.0000000
## C+IAA_SH_M248-C_SH_LA1511 0.509996902 -6.80231289 7.822307 1.0000000
## S_RO_LA1511-C_SH_LA1511 32.835684498 24.39215645 41.279213 0.0000000
## S_RO_M058-C_SH_LA1511 22.029714017 14.27383826 29.785590 0.0000000
## S_RO_M248-C_SH_LA1511 32.027823479 24.27194772 39.783699 0.0000000
## S_SH_LA1511-C_SH_LA1511 41.733624002 33.29009595 50.177152 0.0000000
## S_SH_M058-C_SH_LA1511 45.570557382 37.81468162 53.326433 0.0000000
## S_SH_M248-C_SH_LA1511 53.140129427 45.38425367 60.896005 0.0000000
## S+IAA_RO_LA1511-C_SH_LA1511 39.123799816 31.81149003 46.436110 0.0000000
## S+IAA_RO_M058-C_SH_LA1511 27.465729668 20.15341988 34.778039 0.0000000
## S+IAA_RO_M248-C_SH_LA1511 38.850116674 31.09424092 46.605992 0.0000000
## S+IAA_SH_LA1511-C_SH_LA1511 40.752513352 33.44020356 48.064823 0.0000000
## S+IAA_SH_M058-C_SH_LA1511 51.718798016 44.40648823 59.031108 0.0000000
## S+IAA_SH_M248-C_SH_LA1511 61.378021092 53.62214533 69.133897 0.0000000
## C_SH_M248-C_SH_M058 0.043561083 -7.26874871 7.355871 1.0000000
## C+IAA_RO_LA1511-C_SH_M058 -0.481861920 -7.79417171 6.830448 1.0000000
## C+IAA_RO_M058-C_SH_M058 0.334668931 -6.97764086 7.646979 1.0000000
## C+IAA_RO_M248-C_SH_M058 0.225279457 -7.08703033 7.537589 1.0000000
## C+IAA_SH_LA1511-C_SH_M058 -0.054423804 -7.36673359 7.257886 1.0000000
## C+IAA_SH_M058-C_SH_M058 0.165192052 -7.14711774 7.477502 1.0000000
## C+IAA_SH_M248-C_SH_M058 0.494966287 -6.81734350 7.807276 1.0000000
## S_RO_LA1511-C_SH_M058 32.820653883 24.37712583 41.264182 0.0000000
## S_RO_M058-C_SH_M058 22.014683401 14.25880764 29.770559 0.0000000
## S_RO_M248-C_SH_M058 32.012792864 24.25691711 39.768669 0.0000000
## S_SH_LA1511-C_SH_M058 41.718593386 33.27506534 50.162121 0.0000000
## S_SH_M058-C_SH_M058 45.555526766 37.79965101 53.311403 0.0000000
## S_SH_M248-C_SH_M058 53.125098811 45.36922305 60.880975 0.0000000
## S+IAA_RO_LA1511-C_SH_M058 39.108769200 31.79645941 46.421079 0.0000000
## S+IAA_RO_M058-C_SH_M058 27.450699052 20.13838926 34.763009 0.0000000
## S+IAA_RO_M248-C_SH_M058 38.835086059 31.07921030 46.590962 0.0000000
## S+IAA_SH_LA1511-C_SH_M058 40.737482736 33.42517295 48.049793 0.0000000
## S+IAA_SH_M058-C_SH_M058 51.703767400 44.39145761 59.016077 0.0000000
## S+IAA_SH_M248-C_SH_M058 61.362990476 53.60711472 69.118866 0.0000000
## C+IAA_RO_LA1511-C_SH_M248 -0.525423003 -7.83773279 6.786887 1.0000000
## C+IAA_RO_M058-C_SH_M248 0.291107847 -7.02120194 7.603418 1.0000000
## C+IAA_RO_M248-C_SH_M248 0.181718374 -7.13059142 7.494028 1.0000000
## C+IAA_SH_LA1511-C_SH_M248 -0.097984887 -7.41029468 7.214325 1.0000000
## C+IAA_SH_M058-C_SH_M248 0.121630969 -7.19067882 7.433941 1.0000000
## C+IAA_SH_M248-C_SH_M248 0.451405204 -6.86090459 7.763715 1.0000000
## S_RO_LA1511-C_SH_M248 32.777092800 24.33356475 41.220621 0.0000000
## S_RO_M058-C_SH_M248 21.971122318 14.21524656 29.726998 0.0000000
## S_RO_M248-C_SH_M248 31.969231781 24.21335602 39.725108 0.0000000
## S_SH_LA1511-C_SH_M248 41.675032303 33.23150425 50.118560 0.0000000
## S_SH_M058-C_SH_M248 45.511965683 37.75608993 53.267841 0.0000000
## S_SH_M248-C_SH_M248 53.081537728 45.32566197 60.837413 0.0000000
## S+IAA_RO_LA1511-C_SH_M248 39.065208117 31.75289833 46.377518 0.0000000
## S+IAA_RO_M058-C_SH_M248 27.407137969 20.09482818 34.719448 0.0000000
## S+IAA_RO_M248-C_SH_M248 38.791524976 31.03564922 46.547401 0.0000000
## S+IAA_SH_LA1511-C_SH_M248 40.693921653 33.38161186 48.006231 0.0000000
## S+IAA_SH_M058-C_SH_M248 51.660206317 44.34789653 58.972516 0.0000000
## S+IAA_SH_M248-C_SH_M248 61.319429393 53.56355364 69.075305 0.0000000
## C+IAA_RO_M058-C+IAA_RO_LA1511 0.816530850 -6.49577894 8.128841 1.0000000
## C+IAA_RO_M248-C+IAA_RO_LA1511 0.707141377 -6.60516841 8.019451 1.0000000
## C+IAA_SH_LA1511-C+IAA_RO_LA1511 0.427438115 -6.88487167 7.739748 1.0000000
## C+IAA_SH_M058-C+IAA_RO_LA1511 0.647053972 -6.66525582 7.959364 1.0000000
## C+IAA_SH_M248-C+IAA_RO_LA1511 0.976828207 -6.33548158 8.289138 1.0000000
## S_RO_LA1511-C+IAA_RO_LA1511 33.302515802 24.85898775 41.746044 0.0000000
## S_RO_M058-C+IAA_RO_LA1511 22.496545321 14.74066956 30.252421 0.0000000
## S_RO_M248-C+IAA_RO_LA1511 32.494654783 24.73877903 40.250531 0.0000000
## S_SH_LA1511-C+IAA_RO_LA1511 42.200455306 33.75692726 50.643983 0.0000000
## S_SH_M058-C+IAA_RO_LA1511 46.037388686 38.28151293 53.793264 0.0000000
## S_SH_M248-C+IAA_RO_LA1511 53.606960731 45.85108497 61.362836 0.0000000
## S+IAA_RO_LA1511-C+IAA_RO_LA1511 39.590631120 32.27832133 46.902941 0.0000000
## S+IAA_RO_M058-C+IAA_RO_LA1511 27.932560972 20.62025118 35.244871 0.0000000
## S+IAA_RO_M248-C+IAA_RO_LA1511 39.316947978 31.56107222 47.072824 0.0000000
## S+IAA_SH_LA1511-C+IAA_RO_LA1511 41.219344656 33.90703487 48.531654 0.0000000
## S+IAA_SH_M058-C+IAA_RO_LA1511 52.185629320 44.87331953 59.497939 0.0000000
## S+IAA_SH_M248-C+IAA_RO_LA1511 61.844852396 54.08897664 69.600728 0.0000000
## C+IAA_RO_M248-C+IAA_RO_M058 -0.109389473 -7.42169926 7.202920 1.0000000
## C+IAA_SH_LA1511-C+IAA_RO_M058 -0.389092735 -7.70140252 6.923217 1.0000000
## C+IAA_SH_M058-C+IAA_RO_M058 -0.169476879 -7.48178667 7.142833 1.0000000
## C+IAA_SH_M248-C+IAA_RO_M058 0.160297356 -7.15201243 7.472607 1.0000000
## S_RO_LA1511-C+IAA_RO_M058 32.485984952 24.04245690 40.929513 0.0000000
## S_RO_M058-C+IAA_RO_M058 21.680014471 13.92413871 29.435890 0.0000000
## S_RO_M248-C+IAA_RO_M058 31.678123933 23.92224818 39.434000 0.0000000
## S_SH_LA1511-C+IAA_RO_M058 41.383924456 32.94039641 49.827453 0.0000000
## S_SH_M058-C+IAA_RO_M058 45.220857836 37.46498208 52.976734 0.0000000
## S_SH_M248-C+IAA_RO_M058 52.790429881 45.03455412 60.546306 0.0000000
## S+IAA_RO_LA1511-C+IAA_RO_M058 38.774100270 31.46179048 46.086410 0.0000000
## S+IAA_RO_M058-C+IAA_RO_M058 27.116030122 19.80372033 34.428340 0.0000000
## S+IAA_RO_M248-C+IAA_RO_M058 38.500417128 30.74454137 46.256293 0.0000000
## S+IAA_SH_LA1511-C+IAA_RO_M058 40.402813806 33.09050402 47.715124 0.0000000
## S+IAA_SH_M058-C+IAA_RO_M058 51.369098470 44.05678868 58.681408 0.0000000
## S+IAA_SH_M248-C+IAA_RO_M058 61.028321546 53.27244579 68.784197 0.0000000
## C+IAA_SH_LA1511-C+IAA_RO_M248 -0.279703262 -7.59201305 7.032607 1.0000000
## C+IAA_SH_M058-C+IAA_RO_M248 -0.060087405 -7.37239719 7.252222 1.0000000
## C+IAA_SH_M248-C+IAA_RO_M248 0.269686830 -7.04262296 7.581997 1.0000000
## S_RO_LA1511-C+IAA_RO_M248 32.595374425 24.15184637 41.038902 0.0000000
## S_RO_M058-C+IAA_RO_M248 21.789403944 14.03352819 29.545280 0.0000000
## S_RO_M248-C+IAA_RO_M248 31.787513406 24.03163765 39.543389 0.0000000
## S_SH_LA1511-C+IAA_RO_M248 41.493313929 33.04978588 49.936842 0.0000000
## S_SH_M058-C+IAA_RO_M248 45.330247309 37.57437155 53.086123 0.0000000
## S_SH_M248-C+IAA_RO_M248 52.899819354 45.14394360 60.655695 0.0000000
## S+IAA_RO_LA1511-C+IAA_RO_M248 38.883489743 31.57117995 46.195800 0.0000000
## S+IAA_RO_M058-C+IAA_RO_M248 27.225419595 19.91310981 34.537729 0.0000000
## S+IAA_RO_M248-C+IAA_RO_M248 38.609806601 30.85393084 46.365682 0.0000000
## S+IAA_SH_LA1511-C+IAA_RO_M248 40.512203279 33.19989349 47.824513 0.0000000
## S+IAA_SH_M058-C+IAA_RO_M248 51.478487943 44.16617815 58.790798 0.0000000
## S+IAA_SH_M248-C+IAA_RO_M248 61.137711019 53.38183526 68.893587 0.0000000
## C+IAA_SH_M058-C+IAA_SH_LA1511 0.219615856 -7.09269393 7.531926 1.0000000
## C+IAA_SH_M248-C+IAA_SH_LA1511 0.549390091 -6.76291970 7.861700 1.0000000
## S_RO_LA1511-C+IAA_SH_LA1511 32.875077687 24.43154964 41.318606 0.0000000
## S_RO_M058-C+IAA_SH_LA1511 22.069107205 14.31323145 29.824983 0.0000000
## S_RO_M248-C+IAA_SH_LA1511 32.067216668 24.31134091 39.823092 0.0000000
## S_SH_LA1511-C+IAA_SH_LA1511 41.773017190 33.32948914 50.216545 0.0000000
## S_SH_M058-C+IAA_SH_LA1511 45.609950570 37.85407481 53.365826 0.0000000
## S_SH_M248-C+IAA_SH_LA1511 53.179522615 45.42364686 60.935398 0.0000000
## S+IAA_RO_LA1511-C+IAA_SH_LA1511 39.163193004 31.85088322 46.475503 0.0000000
## S+IAA_RO_M058-C+IAA_SH_LA1511 27.505122856 20.19281307 34.817433 0.0000000
## S+IAA_RO_M248-C+IAA_SH_LA1511 38.889509863 31.13363411 46.645386 0.0000000
## S+IAA_SH_LA1511-C+IAA_SH_LA1511 40.791906540 33.47959675 48.104216 0.0000000
## S+IAA_SH_M058-C+IAA_SH_LA1511 51.758191204 44.44588142 59.070501 0.0000000
## S+IAA_SH_M248-C+IAA_SH_LA1511 61.417414280 53.66153852 69.173290 0.0000000
## C+IAA_SH_M248-C+IAA_SH_M058 0.329774235 -6.98253555 7.642084 1.0000000
## S_RO_LA1511-C+IAA_SH_M058 32.655461831 24.21193378 41.098990 0.0000000
## S_RO_M058-C+IAA_SH_M058 21.849491349 14.09361559 29.605367 0.0000000
## S_RO_M248-C+IAA_SH_M058 31.847600812 24.09172505 39.603477 0.0000000
## S_SH_LA1511-C+IAA_SH_M058 41.553401334 33.10987328 49.996929 0.0000000
## S_SH_M058-C+IAA_SH_M058 45.390334714 37.63445896 53.146210 0.0000000
## S_SH_M248-C+IAA_SH_M058 52.959906759 45.20403100 60.715783 0.0000000
## S+IAA_RO_LA1511-C+IAA_SH_M058 38.943577148 31.63126736 46.255887 0.0000000
## S+IAA_RO_M058-C+IAA_SH_M058 27.285507000 19.97319721 34.597817 0.0000000
## S+IAA_RO_M248-C+IAA_SH_M058 38.669894007 30.91401825 46.425770 0.0000000
## S+IAA_SH_LA1511-C+IAA_SH_M058 40.572290684 33.25998089 47.884600 0.0000000
## S+IAA_SH_M058-C+IAA_SH_M058 51.538575348 44.22626556 58.850885 0.0000000
## S+IAA_SH_M248-C+IAA_SH_M058 61.197798424 53.44192267 68.953674 0.0000000
## S_RO_LA1511-C+IAA_SH_M248 32.325687596 23.88215955 40.769216 0.0000000
## S_RO_M058-C+IAA_SH_M248 21.519717114 13.76384136 29.275593 0.0000000
## S_RO_M248-C+IAA_SH_M248 31.517826577 23.76195082 39.273702 0.0000000
## S_SH_LA1511-C+IAA_SH_M248 41.223627099 32.78009905 49.667155 0.0000000
## S_SH_M058-C+IAA_SH_M248 45.060560479 37.30468472 52.816436 0.0000000
## S_SH_M248-C+IAA_SH_M248 52.630132524 44.87425677 60.386008 0.0000000
## S+IAA_RO_LA1511-C+IAA_SH_M248 38.613802913 31.30149312 45.926113 0.0000000
## S+IAA_RO_M058-C+IAA_SH_M248 26.955732765 19.64342298 34.268043 0.0000000
## S+IAA_RO_M248-C+IAA_SH_M248 38.340119772 30.58424401 46.095996 0.0000000
## S+IAA_SH_LA1511-C+IAA_SH_M248 40.242516449 32.93020666 47.554826 0.0000000
## S+IAA_SH_M058-C+IAA_SH_M248 51.208801113 43.89649132 58.521111 0.0000000
## S+IAA_SH_M248-C+IAA_SH_M248 60.868024189 53.11214843 68.623900 0.0000000
## S_RO_M058-S_RO_LA1511 -10.805970482 -19.63642349 -1.975517 0.0031480
## S_RO_M248-S_RO_LA1511 -0.807861019 -9.63831402 8.022592 1.0000000
## S_SH_LA1511-S_RO_LA1511 8.897939503 -0.54221184 18.338091 0.0912384
## S_SH_M058-S_RO_LA1511 12.734872883 3.90441988 21.565326 0.0001328
## S_SH_M248-S_RO_LA1511 20.304444928 11.47399192 29.134898 0.0000000
## S+IAA_RO_LA1511-S_RO_LA1511 6.288115317 -2.15541273 14.731643 0.4526375
## S+IAA_RO_M058-S_RO_LA1511 -5.369954831 -13.81348288 3.073573 0.7485573
## S+IAA_RO_M248-S_RO_LA1511 6.014432176 -2.81602083 14.844885 0.6292815
## S+IAA_SH_LA1511-S_RO_LA1511 7.916828853 -0.52669920 16.360357 0.0958436
## S+IAA_SH_M058-S_RO_LA1511 18.883113517 10.43958547 27.326642 0.0000000
## S+IAA_SH_M248-S_RO_LA1511 28.542336593 19.71188359 37.372790 0.0000000
## S_RO_M248-S_RO_M058 9.998109463 1.82269858 18.173520 0.0031810
## S_SH_LA1511-S_RO_M058 19.703909985 10.87345698 28.534363 0.0000000
## S_SH_M058-S_RO_M058 23.540843365 15.36543248 31.716254 0.0000000
## S_SH_M248-S_RO_M058 31.110415410 22.93500453 39.285826 0.0000000
## S+IAA_RO_LA1511-S_RO_M058 17.094085799 9.33821004 24.849962 0.0000000
## S+IAA_RO_M058-S_RO_M058 5.436015651 -2.31986011 13.191891 0.5740450
## S+IAA_RO_M248-S_RO_M058 16.820402658 8.64499178 24.995814 0.0000000
## S+IAA_SH_LA1511-S_RO_M058 18.722799335 10.96692358 26.478675 0.0000000
## S+IAA_SH_M058-S_RO_M058 29.689083999 21.93320824 37.444960 0.0000000
## S+IAA_SH_M248-S_RO_M058 39.348307075 31.17289619 47.523718 0.0000000
## S_SH_LA1511-S_RO_M248 9.705800522 0.87534752 18.536254 0.0158143
## S_SH_M058-S_RO_M248 13.542733902 5.36732302 21.718145 0.0000044
## S_SH_M248-S_RO_M248 21.112305947 12.93689507 29.287717 0.0000000
## S+IAA_RO_LA1511-S_RO_M248 7.095976336 -0.65989942 14.851852 0.1195240
## S+IAA_RO_M058-S_RO_M248 -4.562093812 -12.31796957 3.193782 0.8536407
## S+IAA_RO_M248-S_RO_M248 6.822293195 -1.35311769 14.997704 0.2419008
## S+IAA_SH_LA1511-S_RO_M248 8.724689872 0.96881412 16.480566 0.0114827
## S+IAA_SH_M058-S_RO_M248 19.690974536 11.93509878 27.446850 0.0000000
## S+IAA_SH_M248-S_RO_M248 29.350197612 21.17478673 37.525608 0.0000000
## S_SH_M058-S_SH_LA1511 3.836933380 -4.99351962 12.667386 0.9933239
## S_SH_M248-S_SH_LA1511 11.406505425 2.57605242 20.236958 0.0012229
## S+IAA_RO_LA1511-S_SH_LA1511 -2.609824186 -11.05335224 5.833704 0.9999590
## S+IAA_RO_M058-S_SH_LA1511 -14.267894334 -22.71142238 -5.824366 0.0000026
## S+IAA_RO_M248-S_SH_LA1511 -2.883507327 -11.71396033 5.946946 0.9998975
## S+IAA_SH_LA1511-S_SH_LA1511 -0.981110650 -9.42463870 7.462417 1.0000000
## S+IAA_SH_M058-S_SH_LA1511 9.985174014 1.54164596 18.428702 0.0054620
## S+IAA_SH_M248-S_SH_LA1511 19.644397090 10.81394409 28.474850 0.0000000
## S_SH_M248-S_SH_M058 7.569572045 -0.60583884 15.744983 0.1075413
## S+IAA_RO_LA1511-S_SH_M058 -6.446757566 -14.20263332 1.309118 0.2482765
## S+IAA_RO_M058-S_SH_M058 -18.104827714 -25.86070347 -10.348952 0.0000000
## S+IAA_RO_M248-S_SH_M058 -6.720440707 -14.89585159 1.454970 0.2667040
## S+IAA_SH_LA1511-S_SH_M058 -4.818044030 -12.57391979 2.937832 0.7839460
## S+IAA_SH_M058-S_SH_M058 6.148240634 -1.60763512 13.904116 0.3311554
## S+IAA_SH_M248-S_SH_M058 15.807463710 7.63205283 23.982875 0.0000000
## S+IAA_RO_LA1511-S_SH_M248 -14.016329611 -21.77220537 -6.260454 0.0000004
## S+IAA_RO_M058-S_SH_M248 -25.674399759 -33.43027552 -17.918524 0.0000000
## S+IAA_RO_M248-S_SH_M248 -14.290012752 -22.46542363 -6.114602 0.0000010
## S+IAA_SH_LA1511-S_SH_M248 -12.387616075 -20.14349183 -4.631740 0.0000116
## S+IAA_SH_M058-S_SH_M248 -1.421331411 -9.17720717 6.334544 1.0000000
## S+IAA_SH_M248-S_SH_M248 8.237891665 0.06248078 16.413303 0.0459768
## S+IAA_RO_M058-S+IAA_RO_LA1511 -11.658070148 -18.97037994 -4.345760 0.0000122
## S+IAA_RO_M248-S+IAA_RO_LA1511 -0.273683141 -8.02955890 7.482193 1.0000000
## S+IAA_SH_LA1511-S+IAA_RO_LA1511 1.628713536 -5.68359625 8.941023 0.9999999
## S+IAA_SH_M058-S+IAA_RO_LA1511 12.594998200 5.28268841 19.907308 0.0000015
## S+IAA_SH_M248-S+IAA_RO_LA1511 22.254221276 14.49834552 30.010097 0.0000000
## S+IAA_RO_M248-S+IAA_RO_M058 11.384387007 3.62851125 19.140263 0.0000895
## S+IAA_SH_LA1511-S+IAA_RO_M058 13.286783684 5.97447389 20.599093 0.0000003
## S+IAA_SH_M058-S+IAA_RO_M058 24.253068348 16.94075856 31.565378 0.0000000
## S+IAA_SH_M248-S+IAA_RO_M058 33.912291424 26.15641567 41.668167 0.0000000
## S+IAA_SH_LA1511-S+IAA_RO_M248 1.902396677 -5.85347908 9.658272 0.9999994
## S+IAA_SH_M058-S+IAA_RO_M248 12.868681341 5.11280558 20.624557 0.0000043
## S+IAA_SH_M248-S+IAA_RO_M248 22.527904417 14.35249354 30.703315 0.0000000
## S+IAA_SH_M058-S+IAA_SH_LA1511 10.966284664 3.65397487 18.278594 0.0000546
## S+IAA_SH_M248-S+IAA_SH_LA1511 20.625507740 12.86963198 28.381383 0.0000000
## S+IAA_SH_M248-S+IAA_SH_M058 9.659223076 1.90334732 17.415099 0.0023388P8 = Output$GenoConTiss[,'p adj']
stat.test<- multcompLetters(P8)
stat.test## C_RO_M058 C_RO_M248 C_SH_LA1511 C_SH_M058 C_SH_M248
## "a" "a" "a" "a" "a"
## C+IAA_RO_LA1511 C+IAA_RO_M058 C+IAA_RO_M248 C+IAA_SH_LA1511 C+IAA_SH_M058
## "a" "a" "a" "a" "a"
## C+IAA_SH_M248 S_RO_LA1511 S_RO_M058 S_RO_M248 S_SH_LA1511
## "a" "bcd" "e" "bc" "df"
## S_SH_M058 S_SH_M248 S+IAA_RO_LA1511 S+IAA_RO_M058 S+IAA_RO_M248
## "fg" "g" "bdf" "ce" "bdf"
## S+IAA_SH_LA1511 S+IAA_SH_M058 S+IAA_SH_M248 C_RO_LA1511
## "df" "g" "h" "a"test <- as.data.frame(stat.test$Letters)
test$group1 <- rownames(test)
test$group2 <- rownames(test)
colnames(test)[1] <- "Tukey"
testtest$info <- strsplit(test$group1, "_")
test$info[[1]][2]## [1] "RO"test$Accession <- "none"
test$Condition<- "none"
test$Tissue<- "none"
testfor(i in 1:nrow(test)){
test$Condition[i] <- test$info[[i]][1]
test$Tissue[i] <- test$info[[i]][2]
test$Accession[i] <- test$info[[i]][3]
}
test2 <- test[,c(5:7,1)]
test2$group1 <- test2$Condition
test2$group2 <- test2$Condition
ICPNa_content <- ggplot(data = ICP, mapping = aes(x = GenoConTiss, y = Na.con.mg.per.mg.dry.weight, colour = Condition))
Na_content <- Na_content + geom_boxplot(alpha=0.2) + geom_jitter(width=0.1,alpha=0.2)
Na_content <- Na_content + stat_summary(fun=mean, geom="point", shape=95, size=6, color="black", fill="black")
Na_content <- Na_content + scale_color_manual(values = c("blue","blueviolet","red", "deeppink"))
Na_content <- Na_content + ylab("Na content, mg/mg dry weight") + xlab("")
Na_content <- Na_content + theme(axis.text.x = element_text(angle=90, hjust=0.9, vjust=0.5))
Na_content <- Na_content + stat_pvalue_manual(test, label = "Tukey", y.position = 80)
Na_content
#I have to facet grid condition, and use maybe ggerrorplot similar to RSA for duf-5 mutant
Na_content <- ggplot(data = ICP, mapping = aes(x = Condition, y = Na.con.mg.per.mg.dry.weight, colour = Condition))
Na_content <- Na_content + geom_boxplot(alpha=0.2) + geom_jitter(width=0.1,alpha=0.2)
Na_content <- Na_content + facet_grid(Tissue ~ Accession, scales = "free_y")
Na_content <- Na_content + stat_summary(fun=mean, geom="point", shape=95, size=6, color="black", fill="black")
Na_content <- Na_content + ylab("Na content, mg/mg dry weight") + xlab("") + stat_pvalue_manual(test2, label = "Tukey", y.position = 65)
Na_content <- Na_content + theme(axis.text.x = element_text(angle=90, hjust=0.9, vjust=0.5))
Na_content <- Na_content + scale_color_manual(values = c("blue","blueviolet","red", "deeppink"))
Na_content <- Na_content + rremove("legend")
#my_box_plot2 <- my_box_plot2 + stat_pvalue_manual(test, label = "Tukey", y.position = 80)
Na_content
Magda mentioned I can put this nice graph with faciting in illustrator
but transfer the statisticall letter manually.OK, now I am going to
calculate K content:
aov(K.con.mg.per.mg.dry.weight ~ GenoConTiss, data = ICP)## Call:
## aov(formula = K.con.mg.per.mg.dry.weight ~ GenoConTiss, data = ICP)
##
## Terms:
## GenoConTiss Residuals
## Sum of Squares 43236.17 3027.59
## Deg. of Freedom 23 86
##
## Residual standard error: 5.933342
## Estimated effects may be unbalancedOutput <- TukeyHSD(aov(K.con.mg.per.mg.dry.weight ~ GenoConTiss, data = ICP))
Output## Tukey multiple comparisons of means
## 95% family-wise confidence level
##
## Fit: aov(formula = K.con.mg.per.mg.dry.weight ~ GenoConTiss, data = ICP)
##
## $GenoConTiss
## diff lwr upr p adj
## C_RO_M058-C_RO_LA1511 -13.13047418 -27.2328094 0.9718611 0.1022272
## C_RO_M248-C_RO_LA1511 -5.25181439 -19.3541496 8.8505209 0.9992007
## C_SH_LA1511-C_RO_LA1511 2.42950155 -11.6728337 16.5318368 1.0000000
## C_SH_M058-C_RO_LA1511 -1.14447971 -15.2468150 12.9578555 1.0000000
## C_SH_M248-C_RO_LA1511 2.40794300 -11.6943923 16.5102783 1.0000000
## C+IAA_RO_LA1511-C_RO_LA1511 -6.93058985 -21.0329251 7.1717454 0.9718390
## C+IAA_RO_M058-C_RO_LA1511 -16.63339217 -30.7357274 -2.5310569 0.0056901
## C+IAA_RO_M248-C_RO_LA1511 -22.89512710 -36.9974624 -8.7927918 0.0000076
## C+IAA_SH_LA1511-C_RO_LA1511 -28.79795955 -42.9002948 -14.6956243 0.0000000
## C+IAA_SH_M058-C_RO_LA1511 2.87640381 -11.2259314 16.9787391 1.0000000
## C+IAA_SH_M248-C_RO_LA1511 0.18125395 -13.9210813 14.2835892 1.0000000
## S_RO_LA1511-C_RO_LA1511 -32.15778421 -48.4417583 -15.8738101 0.0000000
## S_RO_M058-C_RO_LA1511 -44.87707090 -59.8348562 -29.9192856 0.0000000
## S_RO_M248-C_RO_LA1511 -37.37704142 -52.3348268 -22.4192561 0.0000000
## S_SH_LA1511-C_RO_LA1511 -44.33136577 -60.6153399 -28.0473917 0.0000000
## S_SH_M058-C_RO_LA1511 -52.30166204 -67.2594474 -37.3438767 0.0000000
## S_SH_M248-C_RO_LA1511 -52.17928530 -67.1370706 -37.2215000 0.0000000
## S+IAA_RO_LA1511-C_RO_LA1511 -26.88773178 -40.9900670 -12.7853965 0.0000001
## S+IAA_RO_M058-C_RO_LA1511 -39.74267883 -53.8450141 -25.6403436 0.0000000
## S+IAA_RO_M248-C_RO_LA1511 -30.75718887 -45.7149742 -15.7994035 0.0000000
## S+IAA_SH_LA1511-C_RO_LA1511 -40.37862672 -54.4809620 -26.2762915 0.0000000
## S+IAA_SH_M058-C_RO_LA1511 -52.15705129 -66.2593865 -38.0547160 0.0000000
## S+IAA_SH_M248-C_RO_LA1511 -51.75242937 -66.7102147 -36.7946440 0.0000000
## C_RO_M248-C_RO_M058 7.87865980 -6.2236755 21.9809951 0.9033873
## C_SH_LA1511-C_RO_M058 15.55997573 1.4576405 29.6623110 0.0150129
## C_SH_M058-C_RO_M058 11.98599447 -2.1163408 26.0883297 0.2133766
## C_SH_M248-C_RO_M058 15.53841718 1.4360819 29.6407524 0.0152979
## C+IAA_RO_LA1511-C_RO_M058 6.19988433 -7.9024509 20.3022196 0.9922646
## C+IAA_RO_M058-C_RO_M058 -3.50291798 -17.6052532 10.5994173 0.9999992
## C+IAA_RO_M248-C_RO_M058 -9.76465292 -23.8669882 4.3376823 0.5977896
## C+IAA_SH_LA1511-C_RO_M058 -15.66748537 -29.7698206 -1.5651501 0.0136633
## C+IAA_SH_M058-C_RO_M058 16.00687799 1.9045427 30.1092132 0.0101034
## C+IAA_SH_M248-C_RO_M058 13.31172813 -0.7906071 27.4140634 0.0899906
## S_RO_LA1511-C_RO_M058 -19.02731003 -35.3112841 -2.7433359 0.0065731
## S_RO_M058-C_RO_M058 -31.74659672 -46.7043821 -16.7888114 0.0000000
## S_RO_M248-C_RO_M058 -24.24656723 -39.2043526 -9.2887819 0.0000079
## S_SH_LA1511-C_RO_M058 -31.20089159 -47.4848657 -14.9169175 0.0000001
## S_SH_M058-C_RO_M058 -39.17118786 -54.1289732 -24.2134025 0.0000000
## S_SH_M248-C_RO_M058 -39.04881112 -54.0065965 -24.0910258 0.0000000
## S+IAA_RO_LA1511-C_RO_M058 -13.75725760 -27.8595929 0.3450777 0.0650173
## S+IAA_RO_M058-C_RO_M058 -26.61220465 -40.7145399 -12.5098694 0.0000001
## S+IAA_RO_M248-C_RO_M058 -17.62671469 -32.5845000 -2.6689294 0.0057690
## S+IAA_SH_LA1511-C_RO_M058 -27.24815254 -41.3504878 -13.1458173 0.0000000
## S+IAA_SH_M058-C_RO_M058 -39.02657710 -53.1289124 -24.9242418 0.0000000
## S+IAA_SH_M248-C_RO_M058 -38.62195519 -53.5797405 -23.6641699 0.0000000
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## S+IAA_SH_M058-S_RO_M248 -14.78000987 -29.7377952 0.1777755 0.0568681
## S+IAA_SH_M248-S_RO_M248 -14.37538796 -30.1422781 1.3915022 0.1231779
## S_SH_M058-S_SH_LA1511 -7.97029628 -25.0004840 9.0598915 0.9836282
## S_SH_M248-S_SH_LA1511 -7.84791953 -24.8781073 9.1822682 0.9863219
## S+IAA_RO_LA1511-S_SH_LA1511 17.44363399 1.1596599 33.7276081 0.0221596
## S+IAA_RO_M058-S_SH_LA1511 4.58868693 -11.6952872 20.8726610 0.9999918
## S+IAA_RO_M248-S_SH_LA1511 13.57417690 -3.4560109 30.6043647 0.3210811
## S+IAA_SH_LA1511-S_SH_LA1511 3.95273904 -12.3312351 20.2367132 0.9999995
## S+IAA_SH_M058-S_SH_LA1511 -7.82568552 -24.1096596 8.4582886 0.9779250
## S+IAA_SH_M248-S_SH_LA1511 -7.42106361 -24.4512514 9.6091242 0.9930776
## S_SH_M248-S_SH_M058 0.12237674 -15.6445134 15.8892669 1.0000000
## S+IAA_RO_LA1511-S_SH_M058 25.41393027 10.4561449 40.3717156 0.0000022
## S+IAA_RO_M058-S_SH_M058 12.55898321 -2.3988021 27.5167685 0.2321336
## S+IAA_RO_M248-S_SH_M058 21.54447317 5.7775830 37.3113633 0.0004142
## S+IAA_SH_LA1511-S_SH_M058 11.92303532 -3.0347500 26.8808207 0.3209737
## S+IAA_SH_M058-S_SH_M058 0.14461076 -14.8131746 15.1023961 1.0000000
## S+IAA_SH_M248-S_SH_M058 0.54923267 -15.2176575 16.3161228 1.0000000
## S+IAA_RO_LA1511-S_SH_M248 25.29155352 10.3337682 40.2493389 0.0000025
## S+IAA_RO_M058-S_SH_M248 12.43660647 -2.5211789 27.3943918 0.2478110
## S+IAA_RO_M248-S_SH_M248 21.42209643 5.6552063 37.1889866 0.0004644
## S+IAA_SH_LA1511-S_SH_M248 11.80065858 -3.1571268 26.7584439 0.3400816
## S+IAA_SH_M058-S_SH_M248 0.02223402 -14.9355513 14.9800194 1.0000000
## S+IAA_SH_M248-S_SH_M248 0.42685593 -15.3400342 16.1937461 1.0000000
## S+IAA_RO_M058-S+IAA_RO_LA1511 -12.85494706 -26.9572823 1.2473882 0.1234089
## S+IAA_RO_M248-S+IAA_RO_LA1511 -3.86945709 -18.8272424 11.0883282 0.9999983
## S+IAA_SH_LA1511-S+IAA_RO_LA1511 -13.49089495 -27.5932302 0.6114403 0.0791185
## S+IAA_SH_M058-S+IAA_RO_LA1511 -25.26931951 -39.3716548 -11.1669843 0.0000005
## S+IAA_SH_M248-S+IAA_RO_LA1511 -24.86469760 -39.8224829 -9.9069123 0.0000040
## S+IAA_RO_M248-S+IAA_RO_M058 8.98548996 -5.9722954 23.9432753 0.8288721
## S+IAA_SH_LA1511-S+IAA_RO_M058 -0.63594789 -14.7382831 13.4663874 1.0000000
## S+IAA_SH_M058-S+IAA_RO_M058 -12.41437245 -26.5167077 1.6879628 0.1643854
## S+IAA_SH_M248-S+IAA_RO_M058 -12.00975054 -26.9675359 2.9480348 0.3078095
## S+IAA_SH_LA1511-S+IAA_RO_M248 -9.62143785 -24.5792232 5.3363475 0.7303829
## S+IAA_SH_M058-S+IAA_RO_M248 -21.39986241 -36.3576477 -6.4420771 0.0001581
## S+IAA_SH_M248-S+IAA_RO_M248 -20.99524050 -36.7621306 -5.2283504 0.0006898
## S+IAA_SH_M058-S+IAA_SH_LA1511 -11.77842456 -25.8807598 2.3239107 0.2405130
## S+IAA_SH_M248-S+IAA_SH_LA1511 -11.37380265 -26.3315880 3.5839827 0.4111529
## S+IAA_SH_M248-S+IAA_SH_M058 0.40462191 -14.5531634 15.3624072 1.0000000P7 = Output$GenoConTiss[,'p adj']
stat.test<- multcompLetters(P7)
stat.test## C_RO_M058 C_RO_M248 C_SH_LA1511 C_SH_M058 C_SH_M248
## "abc" "abd" "d" "ad" "d"
## C+IAA_RO_LA1511 C+IAA_RO_M058 C+IAA_RO_M248 C+IAA_SH_LA1511 C+IAA_SH_M058
## "abd" "bce" "cef" "efgh" "d"
## C+IAA_SH_M248 S_RO_LA1511 S_RO_M058 S_RO_M248 S_SH_LA1511
## "ad" "efghi" "ij" "fghij" "gij"
## S_SH_M058 S_SH_M248 S+IAA_RO_LA1511 S+IAA_RO_M058 S+IAA_RO_M248
## "j" "j" "cefh" "ghij" "efghi"
## S+IAA_SH_LA1511 S+IAA_SH_M058 S+IAA_SH_M248 C_RO_LA1511
## "ghij" "j" "j" "ad"test <- as.data.frame(stat.test$Letters)
test$group1 <- rownames(test)
test$group2 <- rownames(test)
colnames(test)[1] <- "Tukey"
testtest$info <- strsplit(test$group1, "_")
test$info[[1]][2]## [1] "RO"test$Accession <- "none"
test$Condition<- "none"
test$Tissue<- "none"
testfor(i in 1:nrow(test)){
test$Condition[i] <- test$info[[i]][1]
test$Tissue[i] <- test$info[[i]][2]
test$Accession[i] <- test$info[[i]][3]
}
test2 <- test[,c(5:7,1)]
test2$group1 <- test2$Condition
test2$group2 <- test2$Condition
ICPK_content <- ggplot(data = ICP, mapping = aes(x = GenoConTiss, y = K.con.mg.per.mg.dry.weight, colour = Condition))
K_content <- K_content + geom_boxplot(alpha=0.2) + geom_jitter(width=0.1,alpha=0.2)
K_content <- K_content + stat_summary(fun=mean, geom="point", shape=95, size=6, color="black", fill="black")
K_content <- K_content + scale_color_manual(values = c("blue","blueviolet","red", "deeppink"))
K_content <- K_content + ylab("K content, mg/mg dry weight") + xlab("")
K_content <- K_content + theme(axis.text.x = element_text(angle=90, hjust=0.9, vjust=0.5))
K_content <- K_content + stat_pvalue_manual(test, label = "Tukey", y.position = 80)
K_content
k_content <- ggplot(data = ICP, mapping = aes(x = Condition, y = K.con.mg.per.mg.dry.weight, colour = Condition))
k_content <- k_content + geom_boxplot(alpha=0.2) + geom_jitter(width=0.1,alpha=0.2)
k_content <- k_content + facet_grid(Tissue ~ Accession)
k_content <- k_content + stat_summary(fun=mean, geom="point", shape=95, size=6, color="black", fill="black")
k_content <- k_content + ylab("K content, mg/mg dry weight") + xlab("") + stat_pvalue_manual(test2, label = "Tukey", y.position = 1.5)
k_content <- k_content + theme(axis.text.x = element_text(angle=90, hjust=0.9, vjust=0.5))
K_content <- K_content + scale_color_manual(values = c("blue","blueviolet","red", "deeppink"))## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.k_content <- k_content + rremove("legend")
k_content
aov(Na.k.ratio ~ All.ID, data = ICP)## Call:
## aov(formula = Na.k.ratio ~ All.ID, data = ICP)
##
## Terms:
## All.ID Residuals
## Sum of Squares 251.18829 2.64765
## Deg. of Freedom 23 86
##
## Residual standard error: 0.1754611
## Estimated effects may be unbalancedOutput <- TukeyHSD(aov(Na.k.ratio ~ All.ID, data = ICP))
Output## Tukey multiple comparisons of means
## 95% family-wise confidence level
##
## Fit: aov(formula = Na.k.ratio ~ All.ID, data = ICP)
##
## $All.ID
## diff lwr upr p adj
## LA1511-RO-C-0.1-LA1511-RO-C 0.0077295914 -0.40930531 0.42476449 1.0000000
## LA1511-RO-S-LA1511-RO-C 1.0446399122 0.56308949 1.52619034 0.0000000
## LA1511-RO-S-0.1-LA1511-RO-C 1.0658264790 0.64879158 1.48286138 0.0000000
## LA1511-SH-C-LA1511-RO-C 0.0105029650 -0.40653193 0.42753786 1.0000000
## LA1511-SH-C-0.1-LA1511-RO-C 0.0370865158 -0.37994838 0.45412142 1.0000000
## LA1511-SH-S-LA1511-RO-C 2.1980434989 1.71649308 2.67959392 0.0000000
## LA1511-SH-S-0.1-LA1511-RO-C 1.7465989268 1.32956403 2.16363383 0.0000000
## M058-RO-C-LA1511-RO-C 0.0055401114 -0.41149479 0.42257501 1.0000000
## M058-RO-C-0.1-LA1511-RO-C 0.0291585324 -0.38787637 0.44619343 1.0000000
## M058-RO-S-LA1511-RO-C 1.1867107350 0.74437843 1.62904304 0.0000000
## M058-RO-S-0.1-LA1511-RO-C 1.1571527848 0.74011789 1.57418768 0.0000000
## M058-SH-C-LA1511-RO-C 0.0122468562 -0.40478804 0.42928176 1.0000000
## M058-SH-C-0.1-LA1511-RO-C 0.0126833450 -0.40435155 0.42971824 1.0000000
## M058-SH-S-LA1511-RO-C 3.8078790027 3.36554669 4.25021131 0.0000000
## M058-SH-S-0.1-LA1511-RO-C 4.2005453258 3.78351043 4.61758023 0.0000000
## M248-RO-C-LA1511-RO-C 0.0067754704 -0.41025943 0.42381037 1.0000000
## M248-RO-C-0.1-LA1511-RO-C 0.0352239056 -0.38181099 0.45225880 1.0000000
## M248-RO-S-LA1511-RO-C 1.2161624742 0.77383017 1.65849478 0.0000000
## M248-RO-S-0.1-LA1511-RO-C 1.1801856902 0.73785338 1.62251800 0.0000000
## M248-SH-C-LA1511-RO-C 0.0112196140 -0.40581529 0.42825451 1.0000000
## M248-SH-C-0.1-LA1511-RO-C 0.0190995058 -0.39793539 0.43613441 1.0000000
## M248-SH-S-LA1511-RO-C 4.3382100407 3.89587773 4.78054235 0.0000000
## M248-SH-S-0.1-LA1511-RO-C 4.8260057645 4.38367346 5.26833807 0.0000000
## LA1511-RO-S-LA1511-RO-C-0.1 1.0369103208 0.55535990 1.51846074 0.0000000
## LA1511-RO-S-0.1-LA1511-RO-C-0.1 1.0580968876 0.64106199 1.47513179 0.0000000
## LA1511-SH-C-LA1511-RO-C-0.1 0.0027733736 -0.41426153 0.41980827 1.0000000
## LA1511-SH-C-0.1-LA1511-RO-C-0.1 0.0293569244 -0.38767797 0.44639182 1.0000000
## LA1511-SH-S-LA1511-RO-C-0.1 2.1903139075 1.70876348 2.67186433 0.0000000
## LA1511-SH-S-0.1-LA1511-RO-C-0.1 1.7388693354 1.32183444 2.15590423 0.0000000
## M058-RO-C-LA1511-RO-C-0.1 -0.0021894800 -0.41922438 0.41484542 1.0000000
## M058-RO-C-0.1-LA1511-RO-C-0.1 0.0214289410 -0.39560596 0.43846384 1.0000000
## M058-RO-S-LA1511-RO-C-0.1 1.1789811435 0.73664884 1.62131345 0.0000000
## M058-RO-S-0.1-LA1511-RO-C-0.1 1.1494231934 0.73238829 1.56645809 0.0000000
## M058-SH-C-LA1511-RO-C-0.1 0.0045172648 -0.41251763 0.42155216 1.0000000
## M058-SH-C-0.1-LA1511-RO-C-0.1 0.0049537536 -0.41208115 0.42198865 1.0000000
## M058-SH-S-LA1511-RO-C-0.1 3.8001494113 3.35781710 4.24248172 0.0000000
## M058-SH-S-0.1-LA1511-RO-C-0.1 4.1928157344 3.77578084 4.60985063 0.0000000
## M248-RO-C-LA1511-RO-C-0.1 -0.0009541210 -0.41798902 0.41608078 1.0000000
## M248-RO-C-0.1-LA1511-RO-C-0.1 0.0274943142 -0.38954059 0.44452921 1.0000000
## M248-RO-S-LA1511-RO-C-0.1 1.2084328828 0.76610057 1.65076519 0.0000000
## M248-RO-S-0.1-LA1511-RO-C-0.1 1.1724560988 0.73012379 1.61478841 0.0000000
## M248-SH-C-LA1511-RO-C-0.1 0.0034900226 -0.41354488 0.42052492 1.0000000
## M248-SH-C-0.1-LA1511-RO-C-0.1 0.0113699144 -0.40566498 0.42840481 1.0000000
## M248-SH-S-LA1511-RO-C-0.1 4.3304804493 3.88814814 4.77281276 0.0000000
## M248-SH-S-0.1-LA1511-RO-C-0.1 4.8182761731 4.37594387 5.26060848 0.0000000
## LA1511-RO-S-0.1-LA1511-RO-S 0.0211865668 -0.46036386 0.50273699 1.0000000
## LA1511-SH-C-LA1511-RO-S -1.0341369472 -1.51568737 -0.55258652 0.0000000
## LA1511-SH-C-0.1-LA1511-RO-S -1.0075533964 -1.48910382 -0.52600297 0.0000000
## LA1511-SH-S-LA1511-RO-S 1.1534035867 0.61501385 1.69179333 0.0000000
## LA1511-SH-S-0.1-LA1511-RO-S 0.7019590146 0.22040859 1.18350944 0.0001046
## M058-RO-C-LA1511-RO-S -1.0390998008 -1.52065022 -0.55754938 0.0000000
## M058-RO-C-0.1-LA1511-RO-S -1.0154813798 -1.49703180 -0.53393096 0.0000000
## M058-RO-S-LA1511-RO-S 0.1420708228 -0.36154666 0.64568831 0.9999916
## M058-RO-S-0.1-LA1511-RO-S 0.1125128726 -0.36903755 0.59406330 0.9999997
## M058-SH-C-LA1511-RO-S -1.0323930560 -1.51394348 -0.55084263 0.0000000
## M058-SH-C-0.1-LA1511-RO-S -1.0319565672 -1.51350699 -0.55040614 0.0000000
## M058-SH-S-LA1511-RO-S 2.7632390905 2.25962160 3.26685658 0.0000000
## M058-SH-S-0.1-LA1511-RO-S 3.1559054136 2.67435499 3.63745584 0.0000000
## M248-RO-C-LA1511-RO-S -1.0378644418 -1.51941486 -0.55631402 0.0000000
## M248-RO-C-0.1-LA1511-RO-S -1.0094160066 -1.49096643 -0.52786558 0.0000000
## M248-RO-S-LA1511-RO-S 0.1715225620 -0.33209492 0.67514005 0.9997976
## M248-RO-S-0.1-LA1511-RO-S 0.1355457780 -0.36807171 0.63916326 0.9999964
## M248-SH-C-LA1511-RO-S -1.0334202982 -1.51497072 -0.55186988 0.0000000
## M248-SH-C-0.1-LA1511-RO-S -1.0255404064 -1.50709083 -0.54398998 0.0000000
## M248-SH-S-LA1511-RO-S 3.2935701285 2.78995264 3.79718762 0.0000000
## M248-SH-S-0.1-LA1511-RO-S 3.7813658523 3.27774837 4.28498334 0.0000000
## LA1511-SH-C-LA1511-RO-S-0.1 -1.0553235140 -1.47235841 -0.63828861 0.0000000
## LA1511-SH-C-0.1-LA1511-RO-S-0.1 -1.0287399632 -1.44577486 -0.61170506 0.0000000
## LA1511-SH-S-LA1511-RO-S-0.1 1.1322170199 0.65066660 1.61376744 0.0000000
## LA1511-SH-S-0.1-LA1511-RO-S-0.1 0.6807724478 0.26373755 1.09780735 0.0000066
## M058-RO-C-LA1511-RO-S-0.1 -1.0602863676 -1.47732127 -0.64325147 0.0000000
## M058-RO-C-0.1-LA1511-RO-S-0.1 -1.0366679466 -1.45370285 -0.61963305 0.0000000
## M058-RO-S-LA1511-RO-S-0.1 0.1208842560 -0.32144805 0.56321656 0.9999953
## M058-RO-S-0.1-LA1511-RO-S-0.1 0.0913263058 -0.32570859 0.50836121 0.9999999
## M058-SH-C-LA1511-RO-S-0.1 -1.0535796228 -1.47061452 -0.63654472 0.0000000
## M058-SH-C-0.1-LA1511-RO-S-0.1 -1.0531431340 -1.47017803 -0.63610823 0.0000000
## M058-SH-S-LA1511-RO-S-0.1 2.7420525237 2.29972022 3.18438483 0.0000000
## M058-SH-S-0.1-LA1511-RO-S-0.1 3.1347188468 2.71768395 3.55175375 0.0000000
## M248-RO-C-LA1511-RO-S-0.1 -1.0590510086 -1.47608591 -0.64201611 0.0000000
## M248-RO-C-0.1-LA1511-RO-S-0.1 -1.0306025734 -1.44763747 -0.61356767 0.0000000
## M248-RO-S-LA1511-RO-S-0.1 0.1503359952 -0.29199631 0.59266830 0.9998042
## M248-RO-S-0.1-LA1511-RO-S-0.1 0.1143592112 -0.32797310 0.55669152 0.9999983
## M248-SH-C-LA1511-RO-S-0.1 -1.0546068650 -1.47164176 -0.63757197 0.0000000
## M248-SH-C-0.1-LA1511-RO-S-0.1 -1.0467269732 -1.46376187 -0.62969207 0.0000000
## M248-SH-S-LA1511-RO-S-0.1 3.2723835617 2.83005125 3.71471587 0.0000000
## M248-SH-S-0.1-LA1511-RO-S-0.1 3.7601792855 3.31784698 4.20251159 0.0000000
## LA1511-SH-C-0.1-LA1511-SH-C 0.0265835508 -0.39045135 0.44361845 1.0000000
## LA1511-SH-S-LA1511-SH-C 2.1875405339 1.70599011 2.66909096 0.0000000
## LA1511-SH-S-0.1-LA1511-SH-C 1.7360959618 1.31906106 2.15313086 0.0000000
## M058-RO-C-LA1511-SH-C -0.0049628536 -0.42199775 0.41207205 1.0000000
## M058-RO-C-0.1-LA1511-SH-C 0.0186555674 -0.39837933 0.43569047 1.0000000
## M058-RO-S-LA1511-SH-C 1.1762077699 0.73387546 1.61854008 0.0000000
## M058-RO-S-0.1-LA1511-SH-C 1.1466498198 0.72961492 1.56368472 0.0000000
## M058-SH-C-LA1511-SH-C 0.0017438912 -0.41529101 0.41877879 1.0000000
## M058-SH-C-0.1-LA1511-SH-C 0.0021803800 -0.41485452 0.41921528 1.0000000
## M058-SH-S-LA1511-SH-C 3.7973760377 3.35504373 4.23970835 0.0000000
## M058-SH-S-0.1-LA1511-SH-C 4.1900423608 3.77300746 4.60707726 0.0000000
## M248-RO-C-LA1511-SH-C -0.0037274946 -0.42076239 0.41330740 1.0000000
## M248-RO-C-0.1-LA1511-SH-C 0.0247209406 -0.39231396 0.44175584 1.0000000
## M248-RO-S-LA1511-SH-C 1.2056595092 0.76332720 1.64799182 0.0000000
## M248-RO-S-0.1-LA1511-SH-C 1.1696827252 0.72735042 1.61201503 0.0000000
## M248-SH-C-LA1511-SH-C 0.0007166490 -0.41631825 0.41775155 1.0000000
## M248-SH-C-0.1-LA1511-SH-C 0.0085965408 -0.40843836 0.42563144 1.0000000
## M248-SH-S-LA1511-SH-C 4.3277070757 3.88537477 4.77003938 0.0000000
## M248-SH-S-0.1-LA1511-SH-C 4.8155027995 4.37317049 5.25783511 0.0000000
## LA1511-SH-S-LA1511-SH-C-0.1 2.1609569831 1.67940656 2.64250741 0.0000000
## LA1511-SH-S-0.1-LA1511-SH-C-0.1 1.7095124110 1.29247751 2.12654731 0.0000000
## M058-RO-C-LA1511-SH-C-0.1 -0.0315464044 -0.44858130 0.38548849 1.0000000
## M058-RO-C-0.1-LA1511-SH-C-0.1 -0.0079279834 -0.42496288 0.40910692 1.0000000
## M058-RO-S-LA1511-SH-C-0.1 1.1496242191 0.70729191 1.59195653 0.0000000
## M058-RO-S-0.1-LA1511-SH-C-0.1 1.1200662690 0.70303137 1.53710117 0.0000000
## M058-SH-C-LA1511-SH-C-0.1 -0.0248396596 -0.44187456 0.39219524 1.0000000
## M058-SH-C-0.1-LA1511-SH-C-0.1 -0.0244031708 -0.44143807 0.39263173 1.0000000
## M058-SH-S-LA1511-SH-C-0.1 3.7707924869 3.32846018 4.21312479 0.0000000
## M058-SH-S-0.1-LA1511-SH-C-0.1 4.1634588100 3.74642391 4.58049371 0.0000000
## M248-RO-C-LA1511-SH-C-0.1 -0.0303110454 -0.44734594 0.38672385 1.0000000
## M248-RO-C-0.1-LA1511-SH-C-0.1 -0.0018626102 -0.41889751 0.41517229 1.0000000
## M248-RO-S-LA1511-SH-C-0.1 1.1790759584 0.73674365 1.62140827 0.0000000
## M248-RO-S-0.1-LA1511-SH-C-0.1 1.1430991744 0.70076687 1.58543148 0.0000000
## M248-SH-C-LA1511-SH-C-0.1 -0.0258669018 -0.44290180 0.39116800 1.0000000
## M248-SH-C-0.1-LA1511-SH-C-0.1 -0.0179870100 -0.43502191 0.39904789 1.0000000
## M248-SH-S-LA1511-SH-C-0.1 4.3011235249 3.85879122 4.74345583 0.0000000
## M248-SH-S-0.1-LA1511-SH-C-0.1 4.7889192487 4.34658694 5.23125156 0.0000000
## LA1511-SH-S-0.1-LA1511-SH-S -0.4514445721 -0.93299499 0.03010585 0.0959764
## M058-RO-C-LA1511-SH-S -2.1925033875 -2.67405381 -1.71095296 0.0000000
## M058-RO-C-0.1-LA1511-SH-S -2.1688849665 -2.65043539 -1.68733454 0.0000000
## M058-RO-S-LA1511-SH-S -1.0113327639 -1.51495025 -0.50771528 0.0000000
## M058-RO-S-0.1-LA1511-SH-S -1.0408907141 -1.52244114 -0.55934029 0.0000000
## M058-SH-C-LA1511-SH-S -2.1857966427 -2.66734707 -1.70424622 0.0000000
## M058-SH-C-0.1-LA1511-SH-S -2.1853601539 -2.66691058 -1.70380973 0.0000000
## M058-SH-S-LA1511-SH-S 1.6098355038 1.10621802 2.11345299 0.0000000
## M058-SH-S-0.1-LA1511-SH-S 2.0025018269 1.52095140 2.48405225 0.0000000
## M248-RO-C-LA1511-SH-S -2.1912680285 -2.67281845 -1.70971761 0.0000000
## M248-RO-C-0.1-LA1511-SH-S -2.1628195933 -2.64437002 -1.68126917 0.0000000
## M248-RO-S-LA1511-SH-S -0.9818810247 -1.48549851 -0.47826354 0.0000000
## M248-RO-S-0.1-LA1511-SH-S -1.0178578087 -1.52147530 -0.51424032 0.0000000
## M248-SH-C-LA1511-SH-S -2.1868238849 -2.66837431 -1.70527346 0.0000000
## M248-SH-C-0.1-LA1511-SH-S -2.1789439931 -2.66049442 -1.69739357 0.0000000
## M248-SH-S-LA1511-SH-S 2.1401665418 1.63654905 2.64378403 0.0000000
## M248-SH-S-0.1-LA1511-SH-S 2.6279622656 2.12434478 3.13157975 0.0000000
## M058-RO-C-LA1511-SH-S-0.1 -1.7410588154 -2.15809371 -1.32402392 0.0000000
## M058-RO-C-0.1-LA1511-SH-S-0.1 -1.7174403944 -2.13447529 -1.30040550 0.0000000
## M058-RO-S-LA1511-SH-S-0.1 -0.5598881919 -1.00222050 -0.11755588 0.0017629
## M058-RO-S-0.1-LA1511-SH-S-0.1 -0.5894461420 -1.00648104 -0.17241124 0.0002054
## M058-SH-C-LA1511-SH-S-0.1 -1.7343520706 -2.15138697 -1.31731717 0.0000000
## M058-SH-C-0.1-LA1511-SH-S-0.1 -1.7339155818 -2.15095048 -1.31688068 0.0000000
## M058-SH-S-LA1511-SH-S-0.1 2.0612800759 1.61894777 2.50361238 0.0000000
## M058-SH-S-0.1-LA1511-SH-S-0.1 2.4539463990 2.03691150 2.87098130 0.0000000
## M248-RO-C-LA1511-SH-S-0.1 -1.7398234564 -2.15685836 -1.32278856 0.0000000
## M248-RO-C-0.1-LA1511-SH-S-0.1 -1.7113750212 -2.12840992 -1.29434012 0.0000000
## M248-RO-S-LA1511-SH-S-0.1 -0.5304364526 -0.97276876 -0.08810414 0.0043818
## M248-RO-S-0.1-LA1511-SH-S-0.1 -0.5664132366 -1.00874554 -0.12408093 0.0014331
## M248-SH-C-LA1511-SH-S-0.1 -1.7353793128 -2.15241421 -1.31834441 0.0000000
## M248-SH-C-0.1-LA1511-SH-S-0.1 -1.7274994210 -2.14453432 -1.31046452 0.0000000
## M248-SH-S-LA1511-SH-S-0.1 2.5916111139 2.14927881 3.03394342 0.0000000
## M248-SH-S-0.1-LA1511-SH-S-0.1 3.0794068377 2.63707453 3.52173915 0.0000000
## M058-RO-C-0.1-M058-RO-C 0.0236184210 -0.39341648 0.44065332 1.0000000
## M058-RO-S-M058-RO-C 1.1811706235 0.73883832 1.62350293 0.0000000
## M058-RO-S-0.1-M058-RO-C 1.1516126734 0.73457777 1.56864757 0.0000000
## M058-SH-C-M058-RO-C 0.0067067448 -0.41032815 0.42374164 1.0000000
## M058-SH-C-0.1-M058-RO-C 0.0071432336 -0.40989167 0.42417813 1.0000000
## M058-SH-S-M058-RO-C 3.8023388913 3.36000658 4.24467120 0.0000000
## M058-SH-S-0.1-M058-RO-C 4.1950052144 3.77797032 4.61204011 0.0000000
## M248-RO-C-M058-RO-C 0.0012353590 -0.41579954 0.41827026 1.0000000
## M248-RO-C-0.1-M058-RO-C 0.0296837942 -0.38735111 0.44671869 1.0000000
## M248-RO-S-M058-RO-C 1.2106223628 0.76829005 1.65295467 0.0000000
## M248-RO-S-0.1-M058-RO-C 1.1746455788 0.73231327 1.61697789 0.0000000
## M248-SH-C-M058-RO-C 0.0056795026 -0.41135540 0.42271440 1.0000000
## M248-SH-C-0.1-M058-RO-C 0.0135593944 -0.40347550 0.43059429 1.0000000
## M248-SH-S-M058-RO-C 4.3326699293 3.89033762 4.77500224 0.0000000
## M248-SH-S-0.1-M058-RO-C 4.8204656531 4.37813335 5.26279796 0.0000000
## M058-RO-S-M058-RO-C-0.1 1.1575522025 0.71521989 1.59988451 0.0000000
## M058-RO-S-0.1-M058-RO-C-0.1 1.1279942524 0.71095935 1.54502915 0.0000000
## M058-SH-C-M058-RO-C-0.1 -0.0169116762 -0.43394658 0.40012322 1.0000000
## M058-SH-C-0.1-M058-RO-C-0.1 -0.0164751874 -0.43351009 0.40055971 1.0000000
## M058-SH-S-M058-RO-C-0.1 3.7787204703 3.33638816 4.22105278 0.0000000
## M058-SH-S-0.1-M058-RO-C-0.1 4.1713867934 3.75435189 4.58842169 0.0000000
## M248-RO-C-M058-RO-C-0.1 -0.0223830620 -0.43941796 0.39465184 1.0000000
## M248-RO-C-0.1-M058-RO-C-0.1 0.0060653732 -0.41096953 0.42310027 1.0000000
## M248-RO-S-M058-RO-C-0.1 1.1870039418 0.74467163 1.62933625 0.0000000
## M248-RO-S-0.1-M058-RO-C-0.1 1.1510271578 0.70869485 1.59335947 0.0000000
## M248-SH-C-M058-RO-C-0.1 -0.0179389184 -0.43497382 0.39909598 1.0000000
## M248-SH-C-0.1-M058-RO-C-0.1 -0.0100590266 -0.42709393 0.40697587 1.0000000
## M248-SH-S-M058-RO-C-0.1 4.3090515083 3.86671920 4.75138382 0.0000000
## M248-SH-S-0.1-M058-RO-C-0.1 4.7968472320 4.35451492 5.23917954 0.0000000
## M058-RO-S-0.1-M058-RO-S -0.0295579502 -0.47189026 0.41277436 1.0000000
## M058-SH-C-M058-RO-S -1.1744638787 -1.61679619 -0.73213157 0.0000000
## M058-SH-C-0.1-M058-RO-S -1.1740273899 -1.61635970 -0.73169508 0.0000000
## M058-SH-S-M058-RO-S 2.6211682678 2.15490908 3.08742746 0.0000000
## M058-SH-S-0.1-M058-RO-S 3.0138345909 2.57150228 3.45616690 0.0000000
## M248-RO-C-M058-RO-S -1.1799352646 -1.62226757 -0.73760296 0.0000000
## M248-RO-C-0.1-M058-RO-S -1.1514868293 -1.59381914 -0.70915452 0.0000000
## M248-RO-S-M058-RO-S 0.0294517392 -0.43680745 0.49571093 1.0000000
## M248-RO-S-0.1-M058-RO-S -0.0065250447 -0.47278424 0.45973415 1.0000000
## M248-SH-C-M058-RO-S -1.1754911209 -1.61782343 -0.73315881 0.0000000
## M248-SH-C-0.1-M058-RO-S -1.1676112291 -1.60994354 -0.72527892 0.0000000
## M248-SH-S-M058-RO-S 3.1514993058 2.68524011 3.61775850 0.0000000
## M248-SH-S-0.1-M058-RO-S 3.6392950295 3.17303584 4.10555422 0.0000000
## M058-SH-C-M058-RO-S-0.1 -1.1449059286 -1.56194083 -0.72787103 0.0000000
## M058-SH-C-0.1-M058-RO-S-0.1 -1.1444694398 -1.56150434 -0.72743454 0.0000000
## M058-SH-S-M058-RO-S-0.1 2.6507262179 2.20839391 3.09305853 0.0000000
## M058-SH-S-0.1-M058-RO-S-0.1 3.0433925410 2.62635764 3.46042744 0.0000000
## M248-RO-C-M058-RO-S-0.1 -1.1503773144 -1.56741221 -0.73334242 0.0000000
## M248-RO-C-0.1-M058-RO-S-0.1 -1.1219288792 -1.53896378 -0.70489398 0.0000000
## M248-RO-S-M058-RO-S-0.1 0.0590096894 -0.38332262 0.50134200 1.0000000
## M248-RO-S-0.1-M058-RO-S-0.1 0.0230329054 -0.41929940 0.46536521 1.0000000
## M248-SH-C-M058-RO-S-0.1 -1.1459331708 -1.56296807 -0.72889827 0.0000000
## M248-SH-C-0.1-M058-RO-S-0.1 -1.1380532790 -1.55508818 -0.72101838 0.0000000
## M248-SH-S-M058-RO-S-0.1 3.1810572559 2.73872495 3.62338956 0.0000000
## M248-SH-S-0.1-M058-RO-S-0.1 3.6688529797 3.22652067 4.11118529 0.0000000
## M058-SH-C-0.1-M058-SH-C 0.0004364888 -0.41659841 0.41747139 1.0000000
## M058-SH-S-M058-SH-C 3.7956321465 3.35329984 4.23796445 0.0000000
## M058-SH-S-0.1-M058-SH-C 4.1882984696 3.77126357 4.60533337 0.0000000
## M248-RO-C-M058-SH-C -0.0054713858 -0.42250629 0.41156351 1.0000000
## M248-RO-C-0.1-M058-SH-C 0.0229770494 -0.39405785 0.44001195 1.0000000
## M248-RO-S-M058-SH-C 1.2039156180 0.76158331 1.64624793 0.0000000
## M248-RO-S-0.1-M058-SH-C 1.1679388340 0.72560653 1.61027114 0.0000000
## M248-SH-C-M058-SH-C -0.0010272422 -0.41806214 0.41600766 1.0000000
## M248-SH-C-0.1-M058-SH-C 0.0068526496 -0.41018225 0.42388755 1.0000000
## M248-SH-S-M058-SH-C 4.3259631845 3.88363088 4.76829549 0.0000000
## M248-SH-S-0.1-M058-SH-C 4.8137589083 4.37142660 5.25609122 0.0000000
## M058-SH-S-M058-SH-C-0.1 3.7951956577 3.35286335 4.23752797 0.0000000
## M058-SH-S-0.1-M058-SH-C-0.1 4.1878619808 3.77082708 4.60489688 0.0000000
## M248-RO-C-M058-SH-C-0.1 -0.0059078746 -0.42294277 0.41112702 1.0000000
## M248-RO-C-0.1-M058-SH-C-0.1 0.0225405606 -0.39449434 0.43957546 1.0000000
## M248-RO-S-M058-SH-C-0.1 1.2034791292 0.76114682 1.64581144 0.0000000
## M248-RO-S-0.1-M058-SH-C-0.1 1.1675023452 0.72517004 1.60983465 0.0000000
## M248-SH-C-M058-SH-C-0.1 -0.0014637310 -0.41849863 0.41557117 1.0000000
## M248-SH-C-0.1-M058-SH-C-0.1 0.0064161608 -0.41061874 0.42345106 1.0000000
## M248-SH-S-M058-SH-C-0.1 4.3255266957 3.88319439 4.76785900 0.0000000
## M248-SH-S-0.1-M058-SH-C-0.1 4.8133224195 4.37099011 5.25565473 0.0000000
## M058-SH-S-0.1-M058-SH-S 0.3926663231 -0.04966598 0.83499863 0.1538293
## M248-RO-C-M058-SH-S -3.8011035323 -4.24343584 -3.35877122 0.0000000
## M248-RO-C-0.1-M058-SH-S -3.7726550971 -4.21498741 -3.33032279 0.0000000
## M248-RO-S-M058-SH-S -2.5917165285 -3.05797572 -2.12545734 0.0000000
## M248-RO-S-0.1-M058-SH-S -2.6276933125 -3.09395250 -2.16143412 0.0000000
## M248-SH-C-M058-SH-S -3.7966593887 -4.23899170 -3.35432708 0.0000000
## M248-SH-C-0.1-M058-SH-S -3.7887794969 -4.23111180 -3.34644719 0.0000000
## M248-SH-S-M058-SH-S 0.5303310380 0.06407185 0.99659023 0.0098046
## M248-SH-S-0.1-M058-SH-S 1.0181267618 0.55186757 1.48438595 0.0000000
## M248-RO-C-M058-SH-S-0.1 -4.1937698554 -4.61080475 -3.77673496 0.0000000
## M248-RO-C-0.1-M058-SH-S-0.1 -4.1653214202 -4.58235632 -3.74828652 0.0000000
## M248-RO-S-M058-SH-S-0.1 -2.9843828516 -3.42671516 -2.54205054 0.0000000
## M248-RO-S-0.1-M058-SH-S-0.1 -3.0203596356 -3.46269194 -2.57802733 0.0000000
## M248-SH-C-M058-SH-S-0.1 -4.1893257118 -4.60636061 -3.77229081 0.0000000
## M248-SH-C-0.1-M058-SH-S-0.1 -4.1814458200 -4.59848072 -3.76441092 0.0000000
## M248-SH-S-M058-SH-S-0.1 0.1376647149 -0.30466759 0.57999702 0.9999539
## M248-SH-S-0.1-M058-SH-S-0.1 0.6254604387 0.18312813 1.06779275 0.0002036
## M248-RO-C-0.1-M248-RO-C 0.0284484352 -0.38858646 0.44548333 1.0000000
## M248-RO-S-M248-RO-C 1.2093870038 0.76705470 1.65171931 0.0000000
## M248-RO-S-0.1-M248-RO-C 1.1734102198 0.73107791 1.61574253 0.0000000
## M248-SH-C-M248-RO-C 0.0044441436 -0.41259076 0.42147904 1.0000000
## M248-SH-C-0.1-M248-RO-C 0.0123240354 -0.40471086 0.42935893 1.0000000
## M248-SH-S-M248-RO-C 4.3314345703 3.88910226 4.77376688 0.0000000
## M248-SH-S-0.1-M248-RO-C 4.8192302941 4.37689799 5.26156260 0.0000000
## M248-RO-S-M248-RO-C-0.1 1.1809385686 0.73860626 1.62327088 0.0000000
## M248-RO-S-0.1-M248-RO-C-0.1 1.1449617846 0.70262948 1.58729409 0.0000000
## M248-SH-C-M248-RO-C-0.1 -0.0240042916 -0.44103919 0.39303061 1.0000000
## M248-SH-C-0.1-M248-RO-C-0.1 -0.0161243998 -0.43315930 0.40091050 1.0000000
## M248-SH-S-M248-RO-C-0.1 4.3029861351 3.86065383 4.74531844 0.0000000
## M248-SH-S-0.1-M248-RO-C-0.1 4.7907818589 4.34844955 5.23311417 0.0000000
## M248-RO-S-0.1-M248-RO-S -0.0359767840 -0.50223598 0.43028241 1.0000000
## M248-SH-C-M248-RO-S -1.2049428602 -1.64727517 -0.76261055 0.0000000
## M248-SH-C-0.1-M248-RO-S -1.1970629684 -1.63939528 -0.75473066 0.0000000
## M248-SH-S-M248-RO-S 3.1220475665 2.65578837 3.58830676 0.0000000
## M248-SH-S-0.1-M248-RO-S 3.6098432903 3.14358410 4.07610248 0.0000000
## M248-SH-C-M248-RO-S-0.1 -1.1689660762 -1.61129838 -0.72663377 0.0000000
## M248-SH-C-0.1-M248-RO-S-0.1 -1.1610861844 -1.60341849 -0.71875388 0.0000000
## M248-SH-S-M248-RO-S-0.1 3.1580243505 2.69176516 3.62428354 0.0000000
## M248-SH-S-0.1-M248-RO-S-0.1 3.6458200743 3.17956088 4.11207927 0.0000000
## M248-SH-C-0.1-M248-SH-C 0.0078798918 -0.40915501 0.42491479 1.0000000
## M248-SH-S-M248-SH-C 4.3269904267 3.88465812 4.76932273 0.0000000
## M248-SH-S-0.1-M248-SH-C 4.8147861505 4.37245384 5.25711846 0.0000000
## M248-SH-S-M248-SH-C-0.1 4.3191105349 3.87677823 4.76144284 0.0000000
## M248-SH-S-0.1-M248-SH-C-0.1 4.8069062587 4.36457395 5.24923857 0.0000000
## M248-SH-S-0.1-M248-SH-S 0.4877957237 0.02153653 0.95405492 0.0297311aov(Na.k.ratio ~ GenoConTiss, data = ICP)## Call:
## aov(formula = Na.k.ratio ~ GenoConTiss, data = ICP)
##
## Terms:
## GenoConTiss Residuals
## Sum of Squares 251.18829 2.64765
## Deg. of Freedom 23 86
##
## Residual standard error: 0.1754611
## Estimated effects may be unbalancedOutput <- TukeyHSD(aov(Na.k.ratio ~ GenoConTiss, data = ICP))
Output## Tukey multiple comparisons of means
## 95% family-wise confidence level
##
## Fit: aov(formula = Na.k.ratio ~ GenoConTiss, data = ICP)
##
## $GenoConTiss
## diff lwr upr p adj
## C_RO_M058-C_RO_LA1511 0.0055401114 -0.41149479 0.42257501 1.0000000
## C_RO_M248-C_RO_LA1511 0.0067754704 -0.41025943 0.42381037 1.0000000
## C_SH_LA1511-C_RO_LA1511 0.0105029650 -0.40653193 0.42753786 1.0000000
## C_SH_M058-C_RO_LA1511 0.0122468562 -0.40478804 0.42928176 1.0000000
## C_SH_M248-C_RO_LA1511 0.0112196140 -0.40581529 0.42825451 1.0000000
## C+IAA_RO_LA1511-C_RO_LA1511 0.0077295914 -0.40930531 0.42476449 1.0000000
## C+IAA_RO_M058-C_RO_LA1511 0.0291585324 -0.38787637 0.44619343 1.0000000
## C+IAA_RO_M248-C_RO_LA1511 0.0352239056 -0.38181099 0.45225880 1.0000000
## C+IAA_SH_LA1511-C_RO_LA1511 0.0370865158 -0.37994838 0.45412142 1.0000000
## C+IAA_SH_M058-C_RO_LA1511 0.0126833450 -0.40435155 0.42971824 1.0000000
## C+IAA_SH_M248-C_RO_LA1511 0.0190995058 -0.39793539 0.43613441 1.0000000
## S_RO_LA1511-C_RO_LA1511 1.0446399122 0.56308949 1.52619034 0.0000000
## S_RO_M058-C_RO_LA1511 1.1867107349 0.74437843 1.62904304 0.0000000
## S_RO_M248-C_RO_LA1511 1.2161624742 0.77383017 1.65849478 0.0000000
## S_SH_LA1511-C_RO_LA1511 2.1980434989 1.71649308 2.67959392 0.0000000
## S_SH_M058-C_RO_LA1511 3.8078790027 3.36554669 4.25021131 0.0000000
## S_SH_M248-C_RO_LA1511 4.3382100407 3.89587773 4.78054235 0.0000000
## S+IAA_RO_LA1511-C_RO_LA1511 1.0658264790 0.64879158 1.48286138 0.0000000
## S+IAA_RO_M058-C_RO_LA1511 1.1571527848 0.74011789 1.57418768 0.0000000
## S+IAA_RO_M248-C_RO_LA1511 1.1801856902 0.73785338 1.62251800 0.0000000
## S+IAA_SH_LA1511-C_RO_LA1511 1.7465989268 1.32956403 2.16363383 0.0000000
## S+IAA_SH_M058-C_RO_LA1511 4.2005453258 3.78351043 4.61758023 0.0000000
## S+IAA_SH_M248-C_RO_LA1511 4.8260057644 4.38367346 5.26833807 0.0000000
## C_RO_M248-C_RO_M058 0.0012353590 -0.41579954 0.41827026 1.0000000
## C_SH_LA1511-C_RO_M058 0.0049628536 -0.41207205 0.42199775 1.0000000
## C_SH_M058-C_RO_M058 0.0067067448 -0.41032815 0.42374164 1.0000000
## C_SH_M248-C_RO_M058 0.0056795026 -0.41135540 0.42271440 1.0000000
## C+IAA_RO_LA1511-C_RO_M058 0.0021894800 -0.41484542 0.41922438 1.0000000
## C+IAA_RO_M058-C_RO_M058 0.0236184210 -0.39341648 0.44065332 1.0000000
## C+IAA_RO_M248-C_RO_M058 0.0296837942 -0.38735111 0.44671869 1.0000000
## C+IAA_SH_LA1511-C_RO_M058 0.0315464044 -0.38548849 0.44858130 1.0000000
## C+IAA_SH_M058-C_RO_M058 0.0071432336 -0.40989167 0.42417813 1.0000000
## C+IAA_SH_M248-C_RO_M058 0.0135593944 -0.40347550 0.43059429 1.0000000
## S_RO_LA1511-C_RO_M058 1.0390998008 0.55754938 1.52065022 0.0000000
## S_RO_M058-C_RO_M058 1.1811706235 0.73883832 1.62350293 0.0000000
## S_RO_M248-C_RO_M058 1.2106223628 0.76829005 1.65295467 0.0000000
## S_SH_LA1511-C_RO_M058 2.1925033875 1.71095296 2.67405381 0.0000000
## S_SH_M058-C_RO_M058 3.8023388913 3.36000658 4.24467120 0.0000000
## S_SH_M248-C_RO_M058 4.3326699293 3.89033762 4.77500224 0.0000000
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## S+IAA_SH_M058-C_RO_M058 4.1950052144 3.77797032 4.61204011 0.0000000
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## C_SH_LA1511-C_RO_M248 0.0037274946 -0.41330740 0.42076239 1.0000000
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## C+IAA_RO_LA1511-C_RO_M248 0.0009541210 -0.41608078 0.41798902 1.0000000
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## C+IAA_SH_M248-C_RO_M248 0.0123240354 -0.40471086 0.42935893 1.0000000
## S_RO_LA1511-C_RO_M248 1.0378644418 0.55631402 1.51941486 0.0000000
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## C+IAA_SH_M248-C_SH_LA1511 0.0085965408 -0.40843836 0.42563144 1.0000000
## S_RO_LA1511-C_SH_LA1511 1.0341369472 0.55258652 1.51568737 0.0000000
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## S+IAA_RO_LA1511-C_SH_LA1511 1.0553235140 0.63828861 1.47235841 0.0000000
## S+IAA_RO_M058-C_SH_LA1511 1.1466498198 0.72961492 1.56368472 0.0000000
## S+IAA_RO_M248-C_SH_LA1511 1.1696827252 0.72735042 1.61201503 0.0000000
## S+IAA_SH_LA1511-C_SH_LA1511 1.7360959618 1.31906106 2.15313086 0.0000000
## S+IAA_SH_M058-C_SH_LA1511 4.1900423608 3.77300746 4.60707726 0.0000000
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## C+IAA_RO_LA1511-C_SH_M058 -0.0045172648 -0.42155216 0.41251763 1.0000000
## C+IAA_RO_M058-C_SH_M058 0.0169116762 -0.40012322 0.43394658 1.0000000
## C+IAA_RO_M248-C_SH_M058 0.0229770494 -0.39405785 0.44001195 1.0000000
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## S_RO_LA1511-C_SH_M058 1.0323930560 0.55084263 1.51394348 0.0000000
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## S_SH_M058-C_SH_M058 3.7956321465 3.35329984 4.23796445 0.0000000
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## S+IAA_RO_LA1511-C_SH_M058 1.0535796228 0.63654472 1.47061452 0.0000000
## S+IAA_RO_M058-C_SH_M058 1.1449059286 0.72787103 1.56194083 0.0000000
## S+IAA_RO_M248-C_SH_M058 1.1679388340 0.72560653 1.61027114 0.0000000
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## S+IAA_SH_M058-C_SH_M058 4.1882984696 3.77126357 4.60533337 0.0000000
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## C+IAA_RO_LA1511-C_SH_M248 -0.0034900226 -0.42052492 0.41354488 1.0000000
## C+IAA_RO_M058-C_SH_M248 0.0179389184 -0.39909598 0.43497382 1.0000000
## C+IAA_RO_M248-C_SH_M248 0.0240042916 -0.39303061 0.44103919 1.0000000
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## C+IAA_SH_M248-C_SH_M248 0.0078798918 -0.40915501 0.42491479 1.0000000
## S_RO_LA1511-C_SH_M248 1.0334202982 0.55186988 1.51497072 0.0000000
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## C+IAA_RO_M058-C+IAA_RO_LA1511 0.0214289410 -0.39560596 0.43846384 1.0000000
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## C+IAA_SH_M058-C+IAA_RO_LA1511 0.0049537536 -0.41208115 0.42198865 1.0000000
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## S_RO_LA1511-C+IAA_RO_LA1511 1.0369103208 0.55535990 1.51846074 0.0000000
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## S_RO_M248-C+IAA_RO_LA1511 1.2084328828 0.76610057 1.65076519 0.0000000
## S_SH_LA1511-C+IAA_RO_LA1511 2.1903139075 1.70876348 2.67186433 0.0000000
## S_SH_M058-C+IAA_RO_LA1511 3.8001494113 3.35781710 4.24248172 0.0000000
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## S+IAA_RO_LA1511-C+IAA_RO_LA1511 1.0580968876 0.64106199 1.47513179 0.0000000
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## S+IAA_SH_M058-C+IAA_RO_LA1511 4.1928157344 3.77578084 4.60985063 0.0000000
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## S_SH_M058-C+IAA_RO_M058 3.7787204703 3.33638816 4.22105278 0.0000000
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## S+IAA_SH_M058-S_RO_M058 3.0138345909 2.57150228 3.45616690 0.0000000
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## S+IAA_SH_M058-S_RO_M248 2.9843828516 2.54205054 3.42671516 0.0000000
## S+IAA_SH_M248-S_RO_M248 3.6098432903 3.14358410 4.07610248 0.0000000
## S_SH_M058-S_SH_LA1511 1.6098355038 1.10621802 2.11345299 0.0000000
## S_SH_M248-S_SH_LA1511 2.1401665418 1.63654905 2.64378403 0.0000000
## S+IAA_RO_LA1511-S_SH_LA1511 -1.1322170199 -1.61376744 -0.65066660 0.0000000
## S+IAA_RO_M058-S_SH_LA1511 -1.0408907141 -1.52244114 -0.55934029 0.0000000
## S+IAA_RO_M248-S_SH_LA1511 -1.0178578087 -1.52147530 -0.51424032 0.0000000
## S+IAA_SH_LA1511-S_SH_LA1511 -0.4514445721 -0.93299499 0.03010585 0.0959764
## S+IAA_SH_M058-S_SH_LA1511 2.0025018269 1.52095140 2.48405225 0.0000000
## S+IAA_SH_M248-S_SH_LA1511 2.6279622656 2.12434478 3.13157975 0.0000000
## S_SH_M248-S_SH_M058 0.5303310380 0.06407185 0.99659023 0.0098046
## S+IAA_RO_LA1511-S_SH_M058 -2.7420525237 -3.18438483 -2.29972022 0.0000000
## S+IAA_RO_M058-S_SH_M058 -2.6507262179 -3.09305853 -2.20839391 0.0000000
## S+IAA_RO_M248-S_SH_M058 -2.6276933125 -3.09395250 -2.16143412 0.0000000
## S+IAA_SH_LA1511-S_SH_M058 -2.0612800759 -2.50361238 -1.61894777 0.0000000
## S+IAA_SH_M058-S_SH_M058 0.3926663231 -0.04966598 0.83499863 0.1538293
## S+IAA_SH_M248-S_SH_M058 1.0181267617 0.55186757 1.48438595 0.0000000
## S+IAA_RO_LA1511-S_SH_M248 -3.2723835617 -3.71471587 -2.83005125 0.0000000
## S+IAA_RO_M058-S_SH_M248 -3.1810572559 -3.62338956 -2.73872495 0.0000000
## S+IAA_RO_M248-S_SH_M248 -3.1580243505 -3.62428354 -2.69176516 0.0000000
## S+IAA_SH_LA1511-S_SH_M248 -2.5916111139 -3.03394342 -2.14927881 0.0000000
## S+IAA_SH_M058-S_SH_M248 -0.1376647149 -0.57999702 0.30466759 0.9999539
## S+IAA_SH_M248-S_SH_M248 0.4877957238 0.02153653 0.95405492 0.0297311
## S+IAA_RO_M058-S+IAA_RO_LA1511 0.0913263058 -0.32570859 0.50836121 0.9999999
## S+IAA_RO_M248-S+IAA_RO_LA1511 0.1143592112 -0.32797310 0.55669152 0.9999983
## S+IAA_SH_LA1511-S+IAA_RO_LA1511 0.6807724478 0.26373755 1.09780735 0.0000066
## S+IAA_SH_M058-S+IAA_RO_LA1511 3.1347188468 2.71768395 3.55175375 0.0000000
## S+IAA_SH_M248-S+IAA_RO_LA1511 3.7601792854 3.31784698 4.20251159 0.0000000
## S+IAA_RO_M248-S+IAA_RO_M058 0.0230329054 -0.41929940 0.46536521 1.0000000
## S+IAA_SH_LA1511-S+IAA_RO_M058 0.5894461420 0.17241124 1.00648104 0.0002054
## S+IAA_SH_M058-S+IAA_RO_M058 3.0433925410 2.62635764 3.46042744 0.0000000
## S+IAA_SH_M248-S+IAA_RO_M058 3.6688529797 3.22652067 4.11118529 0.0000000
## S+IAA_SH_LA1511-S+IAA_RO_M248 0.5664132366 0.12408093 1.00874554 0.0014331
## S+IAA_SH_M058-S+IAA_RO_M248 3.0203596356 2.57802733 3.46269194 0.0000000
## S+IAA_SH_M248-S+IAA_RO_M248 3.6458200742 3.17956088 4.11207927 0.0000000
## S+IAA_SH_M058-S+IAA_SH_LA1511 2.4539463990 2.03691150 2.87098130 0.0000000
## S+IAA_SH_M248-S+IAA_SH_LA1511 3.0794068376 2.63707453 3.52173915 0.0000000
## S+IAA_SH_M248-S+IAA_SH_M058 0.6254604386 0.18312813 1.06779275 0.0002036P9 = Output$GenoConTiss[,'p adj']
stat.test<- multcompLetters(P9)
stat.test## C_RO_M058 C_RO_M248 C_SH_LA1511 C_SH_M058 C_SH_M248
## "a" "a" "a" "a" "a"
## C+IAA_RO_LA1511 C+IAA_RO_M058 C+IAA_RO_M248 C+IAA_SH_LA1511 C+IAA_SH_M058
## "a" "a" "a" "a" "a"
## C+IAA_SH_M248 S_RO_LA1511 S_RO_M058 S_RO_M248 S_SH_LA1511
## "a" "b" "b" "b" "c"
## S_SH_M058 S_SH_M248 S+IAA_RO_LA1511 S+IAA_RO_M058 S+IAA_RO_M248
## "d" "e" "b" "b" "b"
## S+IAA_SH_LA1511 S+IAA_SH_M058 S+IAA_SH_M248 C_RO_LA1511
## "c" "de" "f" "a"test <- as.data.frame(stat.test$Letters)
test$group1 <- rownames(test)
test$group2 <- rownames(test)
colnames(test)[1] <- "Tukey"
testtest$info <- strsplit(test$group1, "_")
test$info[[1]][2]## [1] "RO"test$Accession <- "none"
test$Condition<- "none"
test$Tissue<- "none"
testfor(i in 1:nrow(test)){
test$Condition[i] <- test$info[[i]][1]
test$Tissue[i] <- test$info[[i]][2]
test$Accession[i] <- test$info[[i]][3]
}
test2 <- test[,c(5:7,1)]
test2$group1 <- test2$Condition
test2$group2 <- test2$Condition
ICPNa.K_ratio <- ggplot(data = ICP, mapping = aes(x = GenoConTiss, y = Na.k.ratio, colour = Condition))
Na.K_ratio <- K_content + geom_boxplot(alpha=0.2) + geom_jitter(width=0.1,alpha=0.2)
Na.K_ratio <- Na.K_ratio + stat_summary(fun=mean, geom="point", shape=95, size=6, color="black", fill="black")
Na.K_ratio <- Na.K_ratio + scale_color_manual(values = c("blue","blueviolet","red", "deeppink"))## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.Na.K_ratio <- Na.K_ratio + ylab("Na+/K+ ratio, mg/mg dry weight") + xlab("")
Na.K_ratio <- Na.K_ratio + theme(axis.text.x = element_text(angle=90, hjust=0.9, vjust=0.5))
Na.K_ratio <- Na.K_ratio + stat_pvalue_manual(test, label = "Tukey", y.position = 80)
Na.K_ratio 
Na.K_ratio <- ggplot(data = ICP, mapping = aes(x = Condition, y = Na.k.ratio, colour = Condition))
Na.K_ratio <- Na.K_ratio + geom_boxplot(alpha=0.2) + geom_jitter(width=0.1,alpha=0.2)
Na.K_ratio <- Na.K_ratio + facet_grid(Tissue ~ Accession)
Na.K_ratio <- Na.K_ratio + stat_summary(fun=mean, geom="point", shape=95, size=6, color="black", fill="black")
Na.K_ratio <- Na.K_ratio + ylab("Na+/K+ ratio, mg/mg dry weight") + xlab("") + stat_pvalue_manual(test2, label = "Tukey", y.position = 1.5)
Na.K_ratio <- Na.K_ratio + theme(axis.text.x = element_text(angle=90, hjust=0.9, vjust=0.5))
Na.K_ratio <- Na.K_ratio + scale_color_manual(values = c("blue","blueviolet","red", "deeppink"))
Na.K_ratio <- Na.K_ratio + rremove("legend")
Na.K_ratio
library(cowplot)##
## Attaching package: 'cowplot'## The following object is masked from 'package:ggpubr':
##
## get_legendpdf("202310_RSA_IAA_ICP_M248M058LA1511.pdf", width = 13, height = 5)
plot_grid(Na_content, k_content, Na.K_ratio, labels = c("AUTO"), ncol = 3)
dev.off()## png
## 2aov(DW.mg ~ GenoConTiss, data = ICP)## Call:
## aov(formula = DW.mg ~ GenoConTiss, data = ICP)
##
## Terms:
## GenoConTiss Residuals
## Sum of Squares 32300.91 4998.09
## Deg. of Freedom 23 86
##
## Residual standard error: 7.623476
## Estimated effects may be unbalancedOutput <- TukeyHSD(aov(DW.mg ~ GenoConTiss, data = ICP))
Output## Tukey multiple comparisons of means
## 95% family-wise confidence level
##
## Fit: aov(formula = DW.mg ~ GenoConTiss, data = ICP)
##
## $GenoConTiss
## diff lwr upr p adj
## C_RO_M058-C_RO_LA1511 1.4200000 -16.69943527 19.5394353 1.0000000
## C_RO_M248-C_RO_LA1511 10.6400000 -7.47943527 28.7594353 0.8555214
## C_SH_LA1511-C_RO_LA1511 20.3000000 2.18056473 38.4194353 0.0121598
## C_SH_M058-C_RO_LA1511 31.6600000 13.54056473 49.7794353 0.0000010
## C_SH_M248-C_RO_LA1511 54.5600000 36.44056473 72.6794353 0.0000000
## C+IAA_RO_LA1511-C_RO_LA1511 1.3600000 -16.75943527 19.4794353 1.0000000
## C+IAA_RO_M058-C_RO_LA1511 3.5400000 -14.57943527 21.6594353 1.0000000
## C+IAA_RO_M248-C_RO_LA1511 6.6600000 -11.45943527 24.7794353 0.9993424
## C+IAA_SH_LA1511-C_RO_LA1511 43.2400000 25.12056473 61.3594353 0.0000000
## C+IAA_SH_M058-C_RO_LA1511 37.1400000 19.02056473 55.2594353 0.0000000
## C+IAA_SH_M248-C_RO_LA1511 41.1800000 23.06056473 59.2994353 0.0000000
## S_RO_LA1511-C_RO_LA1511 3.2133333 -17.70918832 24.1358550 1.0000000
## S_RO_M058-C_RO_LA1511 0.3550000 -18.86356332 19.5735633 1.0000000
## S_RO_M248-C_RO_LA1511 1.6550000 -17.56356332 20.8735633 1.0000000
## S_SH_LA1511-C_RO_LA1511 35.3133333 14.39081168 56.2358550 0.0000027
## S_SH_M058-C_RO_LA1511 16.9800000 -2.23856332 36.1985633 0.1597414
## S_SH_M248-C_RO_LA1511 33.4550000 14.23643668 52.6735633 0.0000011
## S+IAA_RO_LA1511-C_RO_LA1511 7.5000000 -10.61943527 25.6194353 0.9964506
## S+IAA_RO_M058-C_RO_LA1511 0.5600000 -17.55943527 18.6794353 1.0000000
## S+IAA_RO_M248-C_RO_LA1511 4.8050000 -14.41356332 24.0235633 0.9999991
## S+IAA_SH_LA1511-C_RO_LA1511 34.5200000 16.40056473 52.6394353 0.0000001
## S+IAA_SH_M058-C_RO_LA1511 18.1000000 -0.01943527 36.2194353 0.0505886
## S+IAA_SH_M248-C_RO_LA1511 38.7050000 19.48643668 57.9235633 0.0000000
## C_RO_M248-C_RO_M058 9.2200000 -8.89943527 27.3394353 0.9595523
## C_SH_LA1511-C_RO_M058 18.8800000 0.76056473 36.9994353 0.0312143
## C_SH_M058-C_RO_M058 30.2400000 12.12056473 48.3594353 0.0000036
## C_SH_M248-C_RO_M058 53.1400000 35.02056473 71.2594353 0.0000000
## C+IAA_RO_LA1511-C_RO_M058 -0.0600000 -18.17943527 18.0594353 1.0000000
## C+IAA_RO_M058-C_RO_M058 2.1200000 -15.99943527 20.2394353 1.0000000
## C+IAA_RO_M248-C_RO_M058 5.2400000 -12.87943527 23.3594353 0.9999870
## C+IAA_SH_LA1511-C_RO_M058 41.8200000 23.70056473 59.9394353 0.0000000
## C+IAA_SH_M058-C_RO_M058 35.7200000 17.60056473 53.8394353 0.0000000
## C+IAA_SH_M248-C_RO_M058 39.7600000 21.64056473 57.8794353 0.0000000
## S_RO_LA1511-C_RO_M058 1.7933333 -19.12918832 22.7158550 1.0000000
## S_RO_M058-C_RO_M058 -1.0650000 -20.28356332 18.1535633 1.0000000
## S_RO_M248-C_RO_M058 0.2350000 -18.98356332 19.4535633 1.0000000
## S_SH_LA1511-C_RO_M058 33.8933333 12.97081168 54.8158550 0.0000081
## S_SH_M058-C_RO_M058 15.5600000 -3.65856332 34.7785633 0.2929435
## S_SH_M248-C_RO_M058 32.0350000 12.81643668 51.2535633 0.0000038
## S+IAA_RO_LA1511-C_RO_M058 6.0800000 -12.03943527 24.1994353 0.9998405
## S+IAA_RO_M058-C_RO_M058 -0.8600000 -18.97943527 17.2594353 1.0000000
## S+IAA_RO_M248-C_RO_M058 3.3850000 -15.83356332 22.6035633 1.0000000
## S+IAA_SH_LA1511-C_RO_M058 33.1000000 14.98056473 51.2194353 0.0000003
## S+IAA_SH_M058-C_RO_M058 16.6800000 -1.43943527 34.7994353 0.1132379
## S+IAA_SH_M248-C_RO_M058 37.2850000 18.06643668 56.5035633 0.0000000
## C_SH_LA1511-C_RO_M248 9.6600000 -8.45943527 27.7794353 0.9363613
## C_SH_M058-C_RO_M248 21.0200000 2.90056473 39.1394353 0.0073304
## C_SH_M248-C_RO_M248 43.9200000 25.80056473 62.0394353 0.0000000
## C+IAA_RO_LA1511-C_RO_M248 -9.2800000 -27.39943527 8.8394353 0.9568242
## C+IAA_RO_M058-C_RO_M248 -7.1000000 -25.21943527 11.0194353 0.9983301
## C+IAA_RO_M248-C_RO_M248 -3.9800000 -22.09943527 14.1394353 0.9999999
## C+IAA_SH_LA1511-C_RO_M248 32.6000000 14.48056473 50.7194353 0.0000004
## C+IAA_SH_M058-C_RO_M248 26.5000000 8.38056473 44.6194353 0.0000972
## C+IAA_SH_M248-C_RO_M248 30.5400000 12.42056473 48.6594353 0.0000028
## S_RO_LA1511-C_RO_M248 -7.4266667 -28.34918832 13.4958550 0.9996131
## S_RO_M058-C_RO_M248 -10.2850000 -29.50356332 8.9335633 0.9340736
## S_RO_M248-C_RO_M248 -8.9850000 -28.20356332 10.2335633 0.9838256
## S_SH_LA1511-C_RO_M248 24.6733333 3.75081168 45.5958550 0.0057056
## S_SH_M058-C_RO_M248 6.3400000 -12.87856332 25.5585633 0.9998789
## S_SH_M248-C_RO_M248 22.8150000 3.59643668 42.0335633 0.0051432
## S+IAA_RO_LA1511-C_RO_M248 -3.1400000 -21.25943527 14.9794353 1.0000000
## S+IAA_RO_M058-C_RO_M248 -10.0800000 -28.19943527 8.0394353 0.9068343
## S+IAA_RO_M248-C_RO_M248 -5.8350000 -25.05356332 13.3835633 0.9999698
## S+IAA_SH_LA1511-C_RO_M248 23.8800000 5.76056473 41.9994353 0.0008407
## S+IAA_SH_M058-C_RO_M248 7.4600000 -10.65943527 25.5794353 0.9966961
## S+IAA_SH_M248-C_RO_M248 28.0650000 8.84643668 47.2835633 0.0001005
## C_SH_M058-C_SH_LA1511 11.3600000 -6.75943527 29.4794353 0.7704726
## C_SH_M248-C_SH_LA1511 34.2600000 16.14056473 52.3794353 0.0000001
## C+IAA_RO_LA1511-C_SH_LA1511 -18.9400000 -37.05943527 -0.8205647 0.0300437
## C+IAA_RO_M058-C_SH_LA1511 -16.7600000 -34.87943527 1.3594353 0.1085079
## C+IAA_RO_M248-C_SH_LA1511 -13.6400000 -31.75943527 4.4794353 0.4311191
## C+IAA_SH_LA1511-C_SH_LA1511 22.9400000 4.82056473 41.0594353 0.0017560
## C+IAA_SH_M058-C_SH_LA1511 16.8400000 -1.27943527 34.9594353 0.1039399
## C+IAA_SH_M248-C_SH_LA1511 20.8800000 2.76056473 38.9994353 0.0080996
## S_RO_LA1511-C_SH_LA1511 -17.0866667 -38.00918832 3.8358550 0.2778755
## S_RO_M058-C_SH_LA1511 -19.9450000 -39.16356332 -0.7264367 0.0327489
## S_RO_M248-C_SH_LA1511 -18.6450000 -37.86356332 0.5735633 0.0687924
## S_SH_LA1511-C_SH_LA1511 15.0133333 -5.90918832 35.9358550 0.5273206
## S_SH_M058-C_SH_LA1511 -3.3200000 -22.53856332 15.8985633 1.0000000
## S_SH_M248-C_SH_LA1511 13.1550000 -6.06356332 32.3735633 0.6198702
## S+IAA_RO_LA1511-C_SH_LA1511 -12.8000000 -30.91943527 5.3194353 0.5585218
## S+IAA_RO_M058-C_SH_LA1511 -19.7400000 -37.85943527 -1.6205647 0.0177988
## S+IAA_RO_M248-C_SH_LA1511 -15.4950000 -34.71356332 3.7235633 0.3003661
## S+IAA_SH_LA1511-C_SH_LA1511 14.2200000 -3.89943527 32.3394353 0.3499747
## S+IAA_SH_M058-C_SH_LA1511 -2.2000000 -20.31943527 15.9194353 1.0000000
## S+IAA_SH_M248-C_SH_LA1511 18.4050000 -0.81356332 37.6235633 0.0782916
## C_SH_M248-C_SH_M058 22.9000000 4.78056473 41.0194353 0.0018110
## C+IAA_RO_LA1511-C_SH_M058 -30.3000000 -48.41943527 -12.1805647 0.0000034
## C+IAA_RO_M058-C_SH_M058 -28.1200000 -46.23943527 -10.0005647 0.0000240
## C+IAA_RO_M248-C_SH_M058 -25.0000000 -43.11943527 -6.8805647 0.0003402
## C+IAA_SH_LA1511-C_SH_M058 11.5800000 -6.53943527 29.6994353 0.7408305
## C+IAA_SH_M058-C_SH_M058 5.4800000 -12.63943527 23.5994353 0.9999717
## C+IAA_SH_M248-C_SH_M058 9.5200000 -8.59943527 27.6394353 0.9445611
## S_RO_LA1511-C_SH_M058 -28.4466667 -49.36918832 -7.5241450 0.0004580
## S_RO_M058-C_SH_M058 -31.3050000 -50.52356332 -12.0864367 0.0000070
## S_RO_M248-C_SH_M058 -30.0050000 -49.22356332 -10.7864367 0.0000207
## S_SH_LA1511-C_SH_M058 3.6533333 -17.26918832 24.5758550 1.0000000
## S_SH_M058-C_SH_M058 -14.6800000 -33.89856332 4.5385633 0.4022257
## S_SH_M248-C_SH_M058 1.7950000 -17.42356332 21.0135633 1.0000000
## S+IAA_RO_LA1511-C_SH_M058 -24.1600000 -42.27943527 -6.0405647 0.0006723
## S+IAA_RO_M058-C_SH_M058 -31.1000000 -49.21943527 -12.9805647 0.0000017
## S+IAA_RO_M248-C_SH_M058 -26.8550000 -46.07356332 -7.6364367 0.0002615
## S+IAA_SH_LA1511-C_SH_M058 2.8600000 -15.25943527 20.9794353 1.0000000
## S+IAA_SH_M058-C_SH_M058 -13.5600000 -31.67943527 4.5594353 0.4428659
## S+IAA_SH_M248-C_SH_M058 7.0450000 -12.17356332 26.2635633 0.9993685
## C+IAA_RO_LA1511-C_SH_M248 -53.2000000 -71.31943527 -35.0805647 0.0000000
## C+IAA_RO_M058-C_SH_M248 -51.0200000 -69.13943527 -32.9005647 0.0000000
## C+IAA_RO_M248-C_SH_M248 -47.9000000 -66.01943527 -29.7805647 0.0000000
## C+IAA_SH_LA1511-C_SH_M248 -11.3200000 -29.43943527 6.7994353 0.7756983
## C+IAA_SH_M058-C_SH_M248 -17.4200000 -35.53943527 0.6994353 0.0753438
## C+IAA_SH_M248-C_SH_M248 -13.3800000 -31.49943527 4.7394353 0.4696825
## S_RO_LA1511-C_SH_M248 -51.3466667 -72.26918832 -30.4241450 0.0000000
## S_RO_M058-C_SH_M248 -54.2050000 -73.42356332 -34.9864367 0.0000000
## S_RO_M248-C_SH_M248 -52.9050000 -72.12356332 -33.6864367 0.0000000
## S_SH_LA1511-C_SH_M248 -19.2466667 -40.16918832 1.6758550 0.1139555
## S_SH_M058-C_SH_M248 -37.5800000 -56.79856332 -18.3614367 0.0000000
## S_SH_M248-C_SH_M248 -21.1050000 -40.32356332 -1.8864367 0.0160036
## S+IAA_RO_LA1511-C_SH_M248 -47.0600000 -65.17943527 -28.9405647 0.0000000
## S+IAA_RO_M058-C_SH_M248 -54.0000000 -72.11943527 -35.8805647 0.0000000
## S+IAA_RO_M248-C_SH_M248 -49.7550000 -68.97356332 -30.5364367 0.0000000
## S+IAA_SH_LA1511-C_SH_M248 -20.0400000 -38.15943527 -1.9205647 0.0145335
## S+IAA_SH_M058-C_SH_M248 -36.4600000 -54.57943527 -18.3405647 0.0000000
## S+IAA_SH_M248-C_SH_M248 -15.8550000 -35.07356332 3.3635633 0.2606823
## C+IAA_RO_M058-C+IAA_RO_LA1511 2.1800000 -15.93943527 20.2994353 1.0000000
## C+IAA_RO_M248-C+IAA_RO_LA1511 5.3000000 -12.81943527 23.4194353 0.9999842
## C+IAA_SH_LA1511-C+IAA_RO_LA1511 41.8800000 23.76056473 59.9994353 0.0000000
## C+IAA_SH_M058-C+IAA_RO_LA1511 35.7800000 17.66056473 53.8994353 0.0000000
## C+IAA_SH_M248-C+IAA_RO_LA1511 39.8200000 21.70056473 57.9394353 0.0000000
## S_RO_LA1511-C+IAA_RO_LA1511 1.8533333 -19.06918832 22.7758550 1.0000000
## S_RO_M058-C+IAA_RO_LA1511 -1.0050000 -20.22356332 18.2135633 1.0000000
## S_RO_M248-C+IAA_RO_LA1511 0.2950000 -18.92356332 19.5135633 1.0000000
## S_SH_LA1511-C+IAA_RO_LA1511 33.9533333 13.03081168 54.8758550 0.0000077
## S_SH_M058-C+IAA_RO_LA1511 15.6200000 -3.59856332 34.8385633 0.2861914
## S_SH_M248-C+IAA_RO_LA1511 32.0950000 12.87643668 51.3135633 0.0000036
## S+IAA_RO_LA1511-C+IAA_RO_LA1511 6.1400000 -11.97943527 24.2594353 0.9998133
## S+IAA_RO_M058-C+IAA_RO_LA1511 -0.8000000 -18.91943527 17.3194353 1.0000000
## S+IAA_RO_M248-C+IAA_RO_LA1511 3.4450000 -15.77356332 22.6635633 1.0000000
## S+IAA_SH_LA1511-C+IAA_RO_LA1511 33.1600000 15.04056473 51.2794353 0.0000002
## S+IAA_SH_M058-C+IAA_RO_LA1511 16.7400000 -1.37943527 34.8594353 0.1096750
## S+IAA_SH_M248-C+IAA_RO_LA1511 37.3450000 18.12643668 56.5635633 0.0000000
## C+IAA_RO_M248-C+IAA_RO_M058 3.1200000 -14.99943527 21.2394353 1.0000000
## C+IAA_SH_LA1511-C+IAA_RO_M058 39.7000000 21.58056473 57.8194353 0.0000000
## C+IAA_SH_M058-C+IAA_RO_M058 33.6000000 15.48056473 51.7194353 0.0000002
## C+IAA_SH_M248-C+IAA_RO_M058 37.6400000 19.52056473 55.7594353 0.0000000
## S_RO_LA1511-C+IAA_RO_M058 -0.3266667 -21.24918832 20.5958550 1.0000000
## S_RO_M058-C+IAA_RO_M058 -3.1850000 -22.40356332 16.0335633 1.0000000
## S_RO_M248-C+IAA_RO_M058 -1.8850000 -21.10356332 17.3335633 1.0000000
## S_SH_LA1511-C+IAA_RO_M058 31.7733333 10.85081168 52.6958550 0.0000406
## S_SH_M058-C+IAA_RO_M058 13.4400000 -5.77856332 32.6585633 0.5784370
## S_SH_M248-C+IAA_RO_M058 29.9150000 10.69643668 49.1335633 0.0000223
## S+IAA_RO_LA1511-C+IAA_RO_M058 3.9600000 -14.15943527 22.0794353 0.9999999
## S+IAA_RO_M058-C+IAA_RO_M058 -2.9800000 -21.09943527 15.1394353 1.0000000
## S+IAA_RO_M248-C+IAA_RO_M058 1.2650000 -17.95356332 20.4835633 1.0000000
## S+IAA_SH_LA1511-C+IAA_RO_M058 30.9800000 12.86056473 49.0994353 0.0000019
## S+IAA_SH_M058-C+IAA_RO_M058 14.5600000 -3.55943527 32.6794353 0.3063593
## S+IAA_SH_M248-C+IAA_RO_M058 35.1650000 15.94643668 54.3835633 0.0000002
## C+IAA_SH_LA1511-C+IAA_RO_M248 36.5800000 18.46056473 54.6994353 0.0000000
## C+IAA_SH_M058-C+IAA_RO_M248 30.4800000 12.36056473 48.5994353 0.0000029
## C+IAA_SH_M248-C+IAA_RO_M248 34.5200000 16.40056473 52.6394353 0.0000001
## S_RO_LA1511-C+IAA_RO_M248 -3.4466667 -24.36918832 17.4758550 1.0000000
## S_RO_M058-C+IAA_RO_M248 -6.3050000 -25.52356332 12.9135633 0.9998893
## S_RO_M248-C+IAA_RO_M248 -5.0050000 -24.22356332 14.2135633 0.9999980
## S_SH_LA1511-C+IAA_RO_M248 28.6533333 7.73081168 49.5758550 0.0003959
## S_SH_M058-C+IAA_RO_M248 10.3200000 -8.89856332 29.5385633 0.9319739
## S_SH_M248-C+IAA_RO_M248 26.7950000 7.57643668 46.0135633 0.0002740
## S+IAA_RO_LA1511-C+IAA_RO_M248 0.8400000 -17.27943527 18.9594353 1.0000000
## S+IAA_RO_M058-C+IAA_RO_M248 -6.1000000 -24.21943527 12.0194353 0.9998318
## S+IAA_RO_M248-C+IAA_RO_M248 -1.8550000 -21.07356332 17.3635633 1.0000000
## S+IAA_SH_LA1511-C+IAA_RO_M248 27.8600000 9.74056473 45.9794353 0.0000301
## S+IAA_SH_M058-C+IAA_RO_M248 11.4400000 -6.67943527 29.5594353 0.7598656
## S+IAA_SH_M248-C+IAA_RO_M248 32.0450000 12.82643668 51.2635633 0.0000037
## C+IAA_SH_M058-C+IAA_SH_LA1511 -6.1000000 -24.21943527 12.0194353 0.9998318
## C+IAA_SH_M248-C+IAA_SH_LA1511 -2.0600000 -20.17943527 16.0594353 1.0000000
## S_RO_LA1511-C+IAA_SH_LA1511 -40.0266667 -60.94918832 -19.1041450 0.0000001
## S_RO_M058-C+IAA_SH_LA1511 -42.8850000 -62.10356332 -23.6664367 0.0000000
## S_RO_M248-C+IAA_SH_LA1511 -41.5850000 -60.80356332 -22.3664367 0.0000000
## S_SH_LA1511-C+IAA_SH_LA1511 -7.9266667 -28.84918832 12.9958550 0.9989711
## S_SH_M058-C+IAA_SH_LA1511 -26.2600000 -45.47856332 -7.0414367 0.0004145
## S_SH_M248-C+IAA_SH_LA1511 -9.7850000 -29.00356332 9.4335633 0.9593127
## S+IAA_RO_LA1511-C+IAA_SH_LA1511 -35.7400000 -53.85943527 -17.6205647 0.0000000
## S+IAA_RO_M058-C+IAA_SH_LA1511 -42.6800000 -60.79943527 -24.5605647 0.0000000
## S+IAA_RO_M248-C+IAA_SH_LA1511 -38.4350000 -57.65356332 -19.2164367 0.0000000
## S+IAA_SH_LA1511-C+IAA_SH_LA1511 -8.7200000 -26.83943527 9.3994353 0.9775796
## S+IAA_SH_M058-C+IAA_SH_LA1511 -25.1400000 -43.25943527 -7.0205647 0.0003032
## S+IAA_SH_M248-C+IAA_SH_LA1511 -4.5350000 -23.75356332 14.6835633 0.9999997
## C+IAA_SH_M248-C+IAA_SH_M058 4.0400000 -14.07943527 22.1594353 0.9999999
## S_RO_LA1511-C+IAA_SH_M058 -33.9266667 -54.84918832 -13.0041450 0.0000079
## S_RO_M058-C+IAA_SH_M058 -36.7850000 -56.00356332 -17.5664367 0.0000001
## S_RO_M248-C+IAA_SH_M058 -35.4850000 -54.70356332 -16.2664367 0.0000002
## S_SH_LA1511-C+IAA_SH_M058 -1.8266667 -22.74918832 19.0958550 1.0000000
## S_SH_M058-C+IAA_SH_M058 -20.1600000 -39.37856332 -0.9414367 0.0287826
## S_SH_M248-C+IAA_SH_M058 -3.6850000 -22.90356332 15.5335633 1.0000000
## S+IAA_RO_LA1511-C+IAA_SH_M058 -29.6400000 -47.75943527 -11.5205647 0.0000062
## S+IAA_RO_M058-C+IAA_SH_M058 -36.5800000 -54.69943527 -18.4605647 0.0000000
## S+IAA_RO_M248-C+IAA_SH_M058 -32.3350000 -51.55356332 -13.1164367 0.0000029
## S+IAA_SH_LA1511-C+IAA_SH_M058 -2.6200000 -20.73943527 15.4994353 1.0000000
## S+IAA_SH_M058-C+IAA_SH_M058 -19.0400000 -37.15943527 -0.9205647 0.0281800
## S+IAA_SH_M248-C+IAA_SH_M058 1.5650000 -17.65356332 20.7835633 1.0000000
## S_RO_LA1511-C+IAA_SH_M248 -37.9666667 -58.88918832 -17.0441450 0.0000003
## S_RO_M058-C+IAA_SH_M248 -40.8250000 -60.04356332 -21.6064367 0.0000000
## S_RO_M248-C+IAA_SH_M248 -39.5250000 -58.74356332 -20.3064367 0.0000000
## S_SH_LA1511-C+IAA_SH_M248 -5.8666667 -26.78918832 15.0558550 0.9999925
## S_SH_M058-C+IAA_SH_M248 -24.2000000 -43.41856332 -4.9814367 0.0019319
## S_SH_M248-C+IAA_SH_M248 -7.7250000 -26.94356332 11.4935633 0.9976167
## S+IAA_RO_LA1511-C+IAA_SH_M248 -33.6800000 -51.79943527 -15.5605647 0.0000002
## S+IAA_RO_M058-C+IAA_SH_M248 -40.6200000 -58.73943527 -22.5005647 0.0000000
## S+IAA_RO_M248-C+IAA_SH_M248 -36.3750000 -55.59356332 -17.1564367 0.0000001
## S+IAA_SH_LA1511-C+IAA_SH_M248 -6.6600000 -24.77943527 11.4594353 0.9993424
## S+IAA_SH_M058-C+IAA_SH_M248 -23.0800000 -41.19943527 -4.9605647 0.0015758
## S+IAA_SH_M248-C+IAA_SH_M248 -2.4750000 -21.69356332 16.7435633 1.0000000
## S_RO_M058-S_RO_LA1511 -2.8583333 -24.73963024 19.0229636 1.0000000
## S_RO_M248-S_RO_LA1511 -1.5583333 -23.43963024 20.3229636 1.0000000
## S_SH_LA1511-S_RO_LA1511 32.1000000 8.70790966 55.4920903 0.0003801
## S_SH_M058-S_RO_LA1511 13.7666667 -8.11463024 35.6479636 0.7652101
## S_SH_M248-S_RO_LA1511 30.2416667 8.36036976 52.1229636 0.0003285
## S+IAA_RO_LA1511-S_RO_LA1511 4.2866667 -16.63585499 25.2091883 1.0000000
## S+IAA_RO_M058-S_RO_LA1511 -2.6533333 -23.57585499 18.2691883 1.0000000
## S+IAA_RO_M248-S_RO_LA1511 1.5916667 -20.28963024 23.4729636 1.0000000
## S+IAA_SH_LA1511-S_RO_LA1511 31.3066667 10.38414501 52.2291883 0.0000576
## S+IAA_SH_M058-S_RO_LA1511 14.8866667 -6.03585499 35.8091883 0.5442529
## S+IAA_SH_M248-S_RO_LA1511 35.4916667 13.61036976 57.3729636 0.0000078
## S_RO_M248-S_RO_M058 1.3000000 -18.95814448 21.5581445 1.0000000
## S_SH_LA1511-S_RO_M058 34.9583333 13.07703643 56.8396302 0.0000116
## S_SH_M058-S_RO_M058 16.6250000 -3.63314448 36.8831445 0.2695350
## S_SH_M248-S_RO_M058 33.1000000 12.84185552 53.3581445 0.0000064
## S+IAA_RO_LA1511-S_RO_M058 7.1450000 -12.07356332 26.3635633 0.9992206
## S+IAA_RO_M058-S_RO_M058 0.2050000 -19.01356332 19.4235633 1.0000000
## S+IAA_RO_M248-S_RO_M058 4.4500000 -15.80814448 24.7081445 0.9999999
## S+IAA_SH_LA1511-S_RO_M058 34.1650000 14.94643668 53.3835633 0.0000006
## S+IAA_SH_M058-S_RO_M058 17.7450000 -1.47356332 36.9635633 0.1102538
## S+IAA_SH_M248-S_RO_M058 38.3500000 18.09185552 58.6081445 0.0000001
## S_SH_LA1511-S_RO_M248 33.6583333 11.77703643 55.5396302 0.0000298
## S_SH_M058-S_RO_M248 15.3250000 -4.93314448 35.5831445 0.4213578
## S_SH_M248-S_RO_M248 31.8000000 11.54185552 52.0581445 0.0000181
## S+IAA_RO_LA1511-S_RO_M248 5.8450000 -13.37356332 25.0635633 0.9999689
## S+IAA_RO_M058-S_RO_M248 -1.0950000 -20.31356332 18.1235633 1.0000000
## S+IAA_RO_M248-S_RO_M248 3.1500000 -17.10814448 23.4081445 1.0000000
## S+IAA_SH_LA1511-S_RO_M248 32.8650000 13.64643668 52.0835633 0.0000018
## S+IAA_SH_M058-S_RO_M248 16.4450000 -2.77356332 35.6635633 0.2033769
## S+IAA_SH_M248-S_RO_M248 37.0500000 16.79185552 57.3081445 0.0000003
## S_SH_M058-S_SH_LA1511 -18.3333333 -40.21463024 3.5479636 0.2354753
## S_SH_M248-S_SH_LA1511 -1.8583333 -23.73963024 20.0229636 1.0000000
## S+IAA_RO_LA1511-S_SH_LA1511 -27.8133333 -48.73585499 -6.8908117 0.0007128
## S+IAA_RO_M058-S_SH_LA1511 -34.7533333 -55.67585499 -13.8308117 0.0000041
## S+IAA_RO_M248-S_SH_LA1511 -30.5083333 -52.38963024 -8.6270364 0.0002739
## S+IAA_SH_LA1511-S_SH_LA1511 -0.7933333 -21.71585499 20.1291883 1.0000000
## S+IAA_SH_M058-S_SH_LA1511 -17.2133333 -38.13585499 3.7091883 0.2652951
## S+IAA_SH_M248-S_SH_LA1511 3.3916667 -18.48963024 25.2729636 1.0000000
## S_SH_M248-S_SH_M058 16.4750000 -3.78314448 36.7331445 0.2851247
## S+IAA_RO_LA1511-S_SH_M058 -9.4800000 -28.69856332 9.7385633 0.9707043
## S+IAA_RO_M058-S_SH_M058 -16.4200000 -35.63856332 2.7985633 0.2056081
## S+IAA_RO_M248-S_SH_M058 -12.1750000 -32.43314448 8.0831445 0.8283154
## S+IAA_SH_LA1511-S_SH_M058 17.5400000 -1.67856332 36.7585633 0.1221157
## S+IAA_SH_M058-S_SH_M058 1.1200000 -18.09856332 20.3385633 1.0000000
## S+IAA_SH_M248-S_SH_M058 21.7250000 1.46685552 41.9831445 0.0218467
## S+IAA_RO_LA1511-S_SH_M248 -25.9550000 -45.17356332 -6.7364367 0.0005235
## S+IAA_RO_M058-S_SH_M248 -32.8950000 -52.11356332 -13.6764367 0.0000018
## S+IAA_RO_M248-S_SH_M248 -28.6500000 -48.90814448 -8.3918555 0.0002028
## S+IAA_SH_LA1511-S_SH_M248 1.0650000 -18.15356332 20.2835633 1.0000000
## S+IAA_SH_M058-S_SH_M248 -15.3550000 -34.57356332 3.8635633 0.3167276
## S+IAA_SH_M248-S_SH_M248 5.2500000 -15.00814448 25.5081445 0.9999982
## S+IAA_RO_M058-S+IAA_RO_LA1511 -6.9400000 -25.05943527 11.1794353 0.9987944
## S+IAA_RO_M248-S+IAA_RO_LA1511 -2.6950000 -21.91356332 16.5235633 1.0000000
## S+IAA_SH_LA1511-S+IAA_RO_LA1511 27.0200000 8.90056473 45.1394353 0.0000623
## S+IAA_SH_M058-S+IAA_RO_LA1511 10.6000000 -7.51943527 28.7194353 0.8596381
## S+IAA_SH_M248-S+IAA_RO_LA1511 31.2050000 11.98643668 50.4235633 0.0000076
## S+IAA_RO_M248-S+IAA_RO_M058 4.2450000 -14.97356332 23.4635633 0.9999999
## S+IAA_SH_LA1511-S+IAA_RO_M058 33.9600000 15.84056473 52.0794353 0.0000001
## S+IAA_SH_M058-S+IAA_RO_M058 17.5400000 -0.57943527 35.6594353 0.0703427
## S+IAA_SH_M248-S+IAA_RO_M058 38.1450000 18.92643668 57.3635633 0.0000000
## S+IAA_SH_LA1511-S+IAA_RO_M248 29.7150000 10.49643668 48.9335633 0.0000263
## S+IAA_SH_M058-S+IAA_RO_M248 13.2950000 -5.92356332 32.5135633 0.5995643
## S+IAA_SH_M248-S+IAA_RO_M248 33.9000000 13.64185552 54.1581445 0.0000034
## S+IAA_SH_M058-S+IAA_SH_LA1511 -16.4200000 -34.53943527 1.6994353 0.1297707
## S+IAA_SH_M248-S+IAA_SH_LA1511 4.1850000 -15.03356332 23.4035633 0.9999999
## S+IAA_SH_M248-S+IAA_SH_M058 20.6050000 1.38643668 39.8235633 0.0219166P6 = Output$GenoConTiss[,'p adj']
stat.test<- multcompLetters(P6)
stat.test## C_RO_M058 C_RO_M248 C_SH_LA1511 C_SH_M058 C_SH_M248
## "a" "ab" "bcd" "cde" "f"
## C+IAA_RO_LA1511 C+IAA_RO_M058 C+IAA_RO_M248 C+IAA_SH_LA1511 C+IAA_SH_M058
## "a" "ab" "ab" "ef" "cef"
## C+IAA_SH_M248 S_RO_LA1511 S_RO_M058 S_RO_M248 S_SH_LA1511
## "ef" "ab" "a" "ab" "cdef"
## S_SH_M058 S_SH_M248 S+IAA_RO_LA1511 S+IAA_RO_M058 S+IAA_RO_M248
## "abd" "cde" "ab" "a" "ab"
## S+IAA_SH_LA1511 S+IAA_SH_M058 S+IAA_SH_M248 C_RO_LA1511
## "cde" "abd" "cef" "a"test <- as.data.frame(stat.test$Letters)
test$group1 <- rownames(test)
test$group2 <- rownames(test)
colnames(test)[1] <- "Tukey"
testDW <- ggplot(data = ICP, mapping = aes(x = GenoConTiss, y = DW.mg, colour = Condition))
DW <- DW + geom_boxplot(alpha=0.2) + geom_jitter(width=0.1,alpha=0.2)
DW <- DW + stat_summary(fun=mean, geom="point", shape=95, size=6, color="black", fill="black")
DW <- DW + scale_color_manual(values = c("blue","blueviolet","red", "deeppink"))
DW <- DW + ylab("Dry weight, mg") + xlab("")
DW <- DW + theme(axis.text.x = element_text(angle=90, hjust=0.9, vjust=0.5))
DW <- DW + stat_pvalue_manual(test, label = "Tukey", y.position = 80)
DW
DW <- ggplot(data = ICP, mapping = aes(x = Condition, y = DW.mg, colour = Condition))
DW <- DW + geom_boxplot(alpha=0.2) + geom_jitter(width=0.1,alpha=0.2)
DW <- DW + facet_grid(Tissue ~ Accession, scales = "free_y")
DW <- DW + stat_summary(fun=mean, geom="point", shape=95, size=6, color="black", fill="black")
DW <- DW + ylab("Dry weight, mg") + xlab("")
DW <- DW + theme(axis.text.x = element_text(angle=90, hjust=0.9, vjust=0.5))
DW <- DW + scale_color_manual(values = c("blue","blueviolet","red", "deeppink"))
DW <- DW + rremove("legend")
DW
FW <- read.csv( "12-16-21RootandShoot-fresh-weight-of-M248M058LA1511fromIAAsaltRSAanalysis-hiIAA-only.csv")
FWFW$GenoConTiss <- paste(FW$Condition, FW$Tissue, FW$Accession, sep="_")
FWaov(Weight.mg ~ GenoConTiss, data = FW)## Call:
## aov(formula = Weight.mg ~ GenoConTiss, data = FW)
##
## Terms:
## GenoConTiss Residuals
## Sum of Squares 6119485 412812
## Deg. of Freedom 23 182
##
## Residual standard error: 47.62559
## Estimated effects may be unbalancedOutput <- TukeyHSD(aov(Weight.mg ~ GenoConTiss, data = FW))
Output## Tukey multiple comparisons of means
## 95% family-wise confidence level
##
## Fit: aov(formula = Weight.mg ~ GenoConTiss, data = FW)
##
## $GenoConTiss
## diff lwr upr p adj
## C_RO_M058-C_RO_LA1511 -25.935000 -126.2352806 74.365281 0.9999992
## C_RO_M248-C_RO_LA1511 59.117778 -39.0159346 157.251490 0.8519831
## C_SH_LA1511-C_RO_LA1511 344.760000 233.4868294 456.033171 0.0000000
## C_SH_M058-C_RO_LA1511 304.702500 204.4022194 405.002781 0.0000000
## C_SH_M248-C_RO_LA1511 492.406667 394.2729543 590.540379 0.0000000
## C+IAA_RO_LA1511-C_RO_LA1511 -39.448889 -137.5826012 58.684823 0.9985112
## C+IAA_RO_M058-C_RO_LA1511 1.410000 -94.9553925 97.775393 1.0000000
## C+IAA_RO_M248-C_RO_LA1511 42.200000 -54.1653925 138.565393 0.9950885
## C+IAA_SH_LA1511-C_RO_LA1511 403.117778 304.9840654 501.251490 0.0000000
## C+IAA_SH_M058-C_RO_LA1511 265.950000 169.5846075 362.315393 0.0000000
## C+IAA_SH_M248-C_RO_LA1511 360.370000 264.0046075 456.735393 0.0000000
## S_RO_LA1511-C_RO_LA1511 -78.826667 -185.3626166 27.709283 0.4947761
## S_RO_M058-C_RO_LA1511 -108.297500 -208.5977806 -7.997219 0.0189027
## S_RO_M248-C_RO_LA1511 -77.147500 -177.4477806 23.152781 0.4137770
## S_SH_LA1511-C_RO_LA1511 81.656667 -24.8792832 188.192617 0.4210350
## S_SH_M058-C_RO_LA1511 -14.422500 -114.7227806 85.877781 1.0000000
## S_SH_M248-C_RO_LA1511 91.415000 -8.8852806 191.715281 0.1288216
## S+IAA_RO_LA1511-C_RO_LA1511 -50.470000 -146.8353925 45.895393 0.9587631
## S+IAA_RO_M058-C_RO_LA1511 -95.060000 -191.4253925 1.305393 0.0583460
## S+IAA_RO_M248-C_RO_LA1511 -64.250000 -160.6153925 32.115393 0.7015278
## S+IAA_SH_LA1511-C_RO_LA1511 94.250000 -2.1153925 190.615393 0.0641030
## S+IAA_SH_M058-C_RO_LA1511 9.640000 -86.7253925 106.005393 1.0000000
## S+IAA_SH_M248-C_RO_LA1511 83.910000 -12.4553925 180.275393 0.1885815
## C_RO_M248-C_RO_M058 85.052778 -0.4378842 170.543440 0.0530259
## C_SH_LA1511-C_RO_M058 370.695000 270.3947194 470.995281 0.0000000
## C_SH_M058-C_RO_M058 330.637500 242.6683346 418.606665 0.0000000
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## S+IAA_SH_M248-S_RO_M058 192.207500 108.7526220 275.662378 0.0000000
## S_SH_LA1511-S_RO_M248 158.804167 63.7866083 253.821725 0.0000011
## S_SH_M058-S_RO_M248 62.725000 -25.2441654 150.694165 0.5717194
## S_SH_M248-S_RO_M248 168.562500 80.5933346 256.531665 0.0000000
## S+IAA_RO_LA1511-S_RO_M248 26.677500 -56.7773780 110.132378 0.9999617
## S+IAA_RO_M058-S_RO_M248 -17.912500 -101.3673780 65.542378 1.0000000
## S+IAA_RO_M248-S_RO_M248 12.897500 -70.5573780 96.352378 1.0000000
## S+IAA_SH_LA1511-S_RO_M248 171.397500 87.9426220 254.852378 0.0000000
## S+IAA_SH_M058-S_RO_M248 86.787500 3.3326220 170.242378 0.0311290
## S+IAA_SH_M248-S_RO_M248 161.057500 77.6026220 244.512378 0.0000000
## S_SH_M058-S_SH_LA1511 -96.079167 -191.0967251 -1.061608 0.0439138
## S_SH_M248-S_SH_LA1511 9.758333 -85.2592251 104.775892 1.0000000
## S+IAA_RO_LA1511-S_SH_LA1511 -132.126667 -222.9808300 -41.272503 0.0000611
## S+IAA_RO_M058-S_SH_LA1511 -176.716667 -267.5708300 -85.862503 0.0000000
## S+IAA_RO_M248-S_SH_LA1511 -145.906667 -236.7608300 -55.052503 0.0000039
## S+IAA_SH_LA1511-S_SH_LA1511 12.593333 -78.2608300 103.447497 1.0000000
## S+IAA_SH_M058-S_SH_LA1511 -72.016667 -162.8708300 18.837497 0.3527998
## S+IAA_SH_M248-S_SH_LA1511 2.253333 -88.6008300 93.107497 1.0000000
## S_SH_M248-S_SH_M058 105.837500 17.8683346 193.806665 0.0034613
## S+IAA_RO_LA1511-S_SH_M058 -36.047500 -119.5023780 47.407378 0.9959157
## S+IAA_RO_M058-S_SH_M058 -80.637500 -164.0923780 2.817378 0.0729999
## S+IAA_RO_M248-S_SH_M058 -49.827500 -133.2823780 33.627378 0.8621180
## S+IAA_SH_LA1511-S_SH_M058 108.672500 25.2176220 192.127378 0.0007661
## S+IAA_SH_M058-S_SH_M058 24.062500 -59.3923780 107.517378 0.9999939
## S+IAA_SH_M248-S_SH_M058 98.332500 14.8776220 181.787378 0.0049562
## S+IAA_RO_LA1511-S_SH_M248 -141.885000 -225.3398780 -58.430122 0.0000007
## S+IAA_RO_M058-S_SH_M248 -186.475000 -269.9298780 -103.020122 0.0000000
## S+IAA_RO_M248-S_SH_M248 -155.665000 -239.1198780 -72.210122 0.0000000
## S+IAA_SH_LA1511-S_SH_M248 2.835000 -80.6198780 86.289878 1.0000000
## S+IAA_SH_M058-S_SH_M248 -81.775000 -165.2298780 1.679878 0.0628153
## S+IAA_SH_M248-S_SH_M248 -7.505000 -90.9598780 75.949878 1.0000000
## S+IAA_RO_M058-S+IAA_RO_LA1511 -44.590000 -123.2720135 34.092013 0.9114861
## S+IAA_RO_M248-S+IAA_RO_LA1511 -13.780000 -92.4620135 64.902013 1.0000000
## S+IAA_SH_LA1511-S+IAA_RO_LA1511 144.720000 66.0379865 223.402013 0.0000000
## S+IAA_SH_M058-S+IAA_RO_LA1511 60.110000 -18.5720135 138.792013 0.4278364
## S+IAA_SH_M248-S+IAA_RO_LA1511 134.380000 55.6979865 213.062013 0.0000006
## S+IAA_RO_M248-S+IAA_RO_M058 30.810000 -47.8720135 109.492013 0.9989885
## S+IAA_SH_LA1511-S+IAA_RO_M058 189.310000 110.6279865 267.992013 0.0000000
## S+IAA_SH_M058-S+IAA_RO_M058 104.700000 26.0179865 183.382013 0.0004860
## S+IAA_SH_M248-S+IAA_RO_M058 178.970000 100.2879865 257.652013 0.0000000
## S+IAA_SH_LA1511-S+IAA_RO_M248 158.500000 79.8179865 237.182013 0.0000000
## S+IAA_SH_M058-S+IAA_RO_M248 73.890000 -4.7920135 152.572013 0.0974288
## S+IAA_SH_M248-S+IAA_RO_M248 148.160000 69.4779865 226.842013 0.0000000
## S+IAA_SH_M058-S+IAA_SH_LA1511 -84.610000 -163.2920135 -5.927987 0.0199973
## S+IAA_SH_M248-S+IAA_SH_LA1511 -10.340000 -89.0220135 68.342013 1.0000000
## S+IAA_SH_M248-S+IAA_SH_M058 74.270000 -4.4120135 152.952013 0.0926486P6 = Output$GenoConTiss[,'p adj']
stat.test<- multcompLetters(P6)
stat.test## C_RO_M058 C_RO_M248 C_SH_LA1511 C_SH_M058 C_SH_M248
## "abcde" "afgh" "ijk" "ij" "l"
## C+IAA_RO_LA1511 C+IAA_RO_M058 C+IAA_RO_M248 C+IAA_SH_LA1511 C+IAA_SH_M058
## "bcde" "abdf" "afgh" "k" "j"
## C+IAA_SH_M248 S_RO_LA1511 S_RO_M058 S_RO_M248 S_SH_LA1511
## "ik" "bcde" "c" "cde" "fgh"
## S_SH_M058 S_SH_M248 S+IAA_RO_LA1511 S+IAA_RO_M058 S+IAA_RO_M248
## "abde" "gh" "bcde" "ce" "bcde"
## S+IAA_SH_LA1511 S+IAA_SH_M058 S+IAA_SH_M248 C_RO_LA1511
## "g" "abfh" "gh" "abdefgh"test <- as.data.frame(stat.test$Letters)
test$group1 <- rownames(test)
test$group2 <- rownames(test)
colnames(test)[1] <- "Tukey"
testFW_all <- ggplot(data = FW, mapping = aes(x = GenoConTiss, y = Weight.mg, colour = Condition))
FW_all <- FW_all + geom_boxplot(alpha=0.2) + geom_jitter(width=0.1,alpha=0.2)
FW_all <- FW_all + stat_summary(fun=mean, geom="point", shape=95, size=6, color="black", fill="black")
FW_all <- FW_all + scale_color_manual(values = c("blue","blueviolet","red", "deeppink"))
FW_all <- FW_all + ylab("Fresh weight, mg") + xlab("")
FW_all <- FW_all + theme(axis.text.x = element_text(angle=90, hjust=0.9, vjust=0.5))
FW_all <- FW_all + stat_pvalue_manual(test, label = "Tukey", y.position = 800)
FW_all
FW_all <- ggplot(data = FW, mapping = aes(x = Condition, y = Weight.mg, colour = Condition))
FW_all <- FW_all + geom_boxplot(alpha=0.2) + geom_jitter(width=0.1,alpha=0.2)
#to assign free scale in root vs shoot use the following code when faceting.
FW_all <- FW_all + facet_grid(Tissue ~ Accession, scales = "free_y")
FW_all <- FW_all + stat_summary(fun=mean, geom="point", shape=95, size=6, color="black", fill="black")
FW_all <- FW_all + scale_color_manual(values = c("blue","blueviolet","cyan", "red", "deeppink", "magenta"))
FW_all <- FW_all + ylab("Fresh weight, mg") + xlab("")
FW_all <- FW_all + theme(axis.text.x = element_text(angle=90, hjust=0.9, vjust=0.5))
FW_all <- FW_all + rremove("legend")
#my_box_plot2 <- my_box_plot2 + stat_pvalue_manual(test, label = "Tukey", y.position = 80)
FW_all 
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