library(seqinr)
dengue=read.fasta("e:/Users/DANILO/Downloads/dengue")
seqden=dengue[[1]]
seqlength=length(seqden)
seqlength
## [1] 10735
dentable=table(seqden)
seqdenG=dentable[[3]]
seqdenG
## [1] 2770
propG=seqdenG/seqlength
propG
## [1] 0.2580345
prop.test(seqdenG,seqlength,alternative = "g",p = 0.25)
##
## 1-sample proportions test with continuity correction
##
## data: seqdenG out of seqlength, null probability 0.25
## X-squared = 3.6531, df = 1, p-value = 0.02798
## alternative hypothesis: true p is greater than 0.25
## 95 percent confidence interval:
## 0.2511035 1.0000000
## sample estimates:
## p
## 0.2580345
la hipotesis nula se rechaza ya que el p-value es menor del 5%
library(seqinr)
dengue2=read.fasta("e:/Users/DANILO/Downloads/dengue 2")
seqden2=dengue2[[1]]
seqlength2=length(seqden2)
seqlength2
## [1] 10723
tableden=count(seqden, 2)
TGden=tableden[[15]]
TGden
## [1] 832
tableden2=count(seqden2,2)
TGden2=tableden2[[15]]
TGden2
## [1] 795
prop.test(x = c(TGden,TGden2),n = c(seqlength,seqlength2),alternative = "two.sided")
##
## 2-sample test for equality of proportions with continuity correction
##
## data: c(TGden, TGden2) out of c(seqlength, seqlength2)
## X-squared = 0.81889, df = 1, p-value = 0.3655
## alternative hypothesis: two.sided
## 95 percent confidence interval:
## -0.003812892 0.010540479
## sample estimates:
## prop 1 prop 2
## 0.07750349 0.07413970
la hipotesis nula no se rechaza ya que el p-value es mayor al 5%
sec=seqden[400:550]
sec
## [1] "g" "a" "c" "c" "a" "t" "g" "c" "t" "c" "c" "t" "c" "a" "t" "g" "c" "t"
## [19] "g" "c" "t" "g" "c" "c" "c" "a" "c" "a" "g" "c" "c" "c" "t" "g" "g" "c"
## [37] "g" "t" "t" "c" "c" "a" "t" "c" "t" "g" "a" "c" "c" "a" "c" "c" "c" "g"
## [55] "a" "g" "g" "g" "g" "g" "a" "g" "a" "g" "c" "c" "g" "c" "a" "c" "a" "t"
## [73] "g" "a" "t" "a" "g" "t" "t" "a" "g" "c" "a" "a" "g" "c" "a" "g" "g" "a"
## [91] "a" "a" "g" "a" "g" "g" "a" "a" "a" "a" "t" "c" "a" "c" "t" "t" "t" "t"
## [109] "g" "t" "t" "t" "a" "a" "g" "a" "c" "c" "t" "c" "t" "g" "c" "a" "g" "g"
## [127] "t" "g" "t" "c" "a" "a" "c" "a" "t" "g" "t" "g" "c" "a" "c" "c" "c" "t"
## [145] "t" "a" "t" "t" "g" "c" "a"
length(sec)
## [1] 151
cuentaden=count(sec,1)
Gden=cuentaden[[3]]
Tden=cuentaden[[4]]
CocienteTG=Tden/Gden
CocienteTG
## [1] 0.8918919
prop.test(x = c(Gden,Tden),n = c(151,151), alternative = "g")
##
## 2-sample test for equality of proportions with continuity correction
##
## data: c(Gden, Tden) out of c(151, 151)
## X-squared = 0.16736, df = 1, p-value = 0.3412
## alternative hypothesis: greater
## 95 percent confidence interval:
## -0.05997342 1.00000000
## sample estimates:
## prop 1 prop 2
## 0.2450331 0.2185430
la hipotesis nula no se rechaza ya que el p-value es mayor al 5%
library(seqinr)
secden=seqden[400:500]
secden
## [1] "g" "a" "c" "c" "a" "t" "g" "c" "t" "c" "c" "t" "c" "a" "t" "g" "c" "t"
## [19] "g" "c" "t" "g" "c" "c" "c" "a" "c" "a" "g" "c" "c" "c" "t" "g" "g" "c"
## [37] "g" "t" "t" "c" "c" "a" "t" "c" "t" "g" "a" "c" "c" "a" "c" "c" "c" "g"
## [55] "a" "g" "g" "g" "g" "g" "a" "g" "a" "g" "c" "c" "g" "c" "a" "c" "a" "t"
## [73] "g" "a" "t" "a" "g" "t" "t" "a" "g" "c" "a" "a" "g" "c" "a" "g" "g" "a"
## [91] "a" "a" "g" "a" "g" "g" "a" "a" "a" "a" "t"
tabladen=count(secden,2)
tabladen
##
## aa ac ag at ca cc cg ct ga gc gg gt ta tc tg tt
## 6 5 10 6 10 11 3 6 9 10 7 2 2 4 7 2
secden2=seqden2[400:500]
secden2
## [1] "g" "c" "a" "g" "g" "c" "a" "t" "g" "a" "t" "c" "a" "t" "t" "a" "t" "g"
## [19] "c" "t" "g" "a" "t" "t" "c" "c" "a" "a" "c" "a" "g" "t" "g" "a" "t" "g"
## [37] "g" "c" "g" "t" "t" "c" "c" "a" "t" "t" "t" "a" "a" "c" "c" "a" "c" "a"
## [55] "c" "g" "t" "a" "a" "c" "g" "g" "a" "g" "a" "a" "c" "c" "a" "c" "a" "c"
## [73] "a" "t" "g" "a" "t" "c" "g" "t" "c" "a" "g" "c" "a" "g" "a" "c" "a" "a"
## [91] "g" "a" "g" "a" "a" "a" "g" "g" "g" "a" "a"
tabladen2=count(secden2,2)
tabladen2
##
## aa ac ag at ca cc cg ct ga gc gg gt ta tc tg tt
## 8 9 8 9 14 4 4 1 10 5 5 4 3 5 6 5
TA=tabladen[[13]]
GC=tabladen[[10]]
TA2=tabladen2[[13]]
GC2=tabladen2[[10]]
cociente=TA/GC
cociente
## [1] 0.2
cociente2=TA2/GC2
cociente2
## [1] 0.6
prop.test(x=c(TA,TA2),n=c(GC,GC2),alternative = "g")
## Warning in prop.test(x = c(TA, TA2), n = c(GC, GC2), alternative = "g"): Chi-
## squared approximation may be incorrect
##
## 2-sample test for equality of proportions with continuity correction
##
## data: c(TA, TA2) out of c(GC, GC2)
## X-squared = 0.9375, df = 1, p-value = 0.8335
## alternative hypothesis: greater
## 95 percent confidence interval:
## -0.9661187 1.0000000
## sample estimates:
## prop 1 prop 2
## 0.2 0.6
la hipotesis nula no se rechaza ya que el p-value es mayor a 5%