Data Import and processing

Libraries:

Replace with agent names:

Calculate means:

Calculate linear models:

Create dfs for plot:

Results

Plot:

Workshop

Calculate SA:

SA <- function(h, v){
  a <- h
  b <- v
  sa <- 2 * pi * (a^2 + a*b*(1.2))
  sa
}

SA of a 12μm x 28μm Tertrahymena: 3438 μm2

Calculate phagosome SA:

pSA <- function(d){
  r <- d/6/2
  psa <- 4 * pi * r^2
  psa
}

SA of a phagosode in a 24μm2 Tertrahymena: 50 μm2

Rate of membrane turnover

turnovers <- round(as.numeric(uptakes) * pSA(24),1)
Agent Uptake (particles/min) Membrane turnover (μm2min-1)
Control 0.309 15.5
DMSO 0.338 17
Nocodazole 0.287 14.4
Pitstop-2 0.251 12.6
Dynasore 0.338 17