1 Biplot Setup

2 Set up

2.1 1) Load packages

2.2 2) Import data

##  [1] "sample_name"       "location"          "treatment"        
##  [4] "clay"              "t_np"              "to_cp"            
##  [7] "ca"                "cu"                "mg"               
## [10] "mn"                "na"                "p"                
## [13] "p_h"               "k"                 "zn"               
## [16] "fe"                "cec"               "gwc"              
## [19] "bd"                "ac"                "x20wsa2000"       
## [22] "respiration"       "mb"                "gramp"            
## [25] "gramn"             "actin"             "amf"              
## [28] "fungi"             "fb"                "glucosidase"      
## [31] "glucosaminidase"   "acidphosphotase"   "alkphosphatase"   
## [34] "arylsulfatase"     "phosphodiesterase" "protein"
##  [1] "sample_name" "location"    "treatment"   "Clay"        "TN"         
##  [6] "SOC"         "Ca"          "Cu"          "Mg"          "Mn"         
## [11] "Na"          "P"           "pH"          "K"           "Zn"         
## [16] "Fe"          "CEC"         "GWC"         "BD"          "POXC"       
## [21] "MWD"         "Resp"        "MB"          "GmP"         "GmN"        
## [26] "Act"         "AMF"         "Fun"         "FB"          "bG"         
## [31] "NAG"         "AP"          "ALK"         "ARY"         "PHO"        
## [36] "Pro"

2.3 4) Theme James

2.4 Overall PCA

## Standard deviations (1, .., p=33):
##  [1] 3.615159e+00 2.418593e+00 1.945146e+00 1.440160e+00 1.217822e+00
##  [6] 1.053898e+00 9.659879e-01 9.134885e-01 8.359385e-01 7.578631e-01
## [11] 6.534546e-01 6.180760e-01 5.785962e-01 5.183776e-01 4.641616e-01
## [16] 3.842594e-01 3.743173e-01 3.631148e-01 3.449183e-01 2.704280e-01
## [21] 2.550464e-01 2.359468e-01 2.177108e-01 2.000767e-01 1.813732e-01
## [26] 1.762910e-01 1.590204e-01 1.354248e-01 1.234781e-01 9.618431e-02
## [31] 7.227879e-02 6.079750e-02 3.665045e-16
## 
## Rotation (n x k) = (33 x 33):
##                PC1          PC2          PC3          PC4          PC5
## Clay -0.0763302839 -0.285472857  0.054966695 -0.057845085  0.035819131
## TN   -0.2431068930  0.113620298 -0.099521649  0.091626726  0.037468099
## SOC  -0.2555224984  0.065372278 -0.086651555 -0.008639296  0.013872651
## Ca   -0.0235374295 -0.351019373 -0.195101340  0.057903378  0.154276259
## Cu   -0.0627234025  0.009318035  0.316157281 -0.080577266  0.447140465
## Mg   -0.0484038078 -0.016923672  0.401583035  0.146274996 -0.200161801
## Mn   -0.0478045471  0.325639548 -0.046019083 -0.154866657  0.281345272
## Na   -0.0719569820 -0.141668078  0.255466337 -0.330664653 -0.061525699
## P     0.1153645930  0.222144158 -0.267378312 -0.260041396  0.173224678
## pH   -0.0164468048 -0.320503889 -0.279357155  0.065009156 -0.104207669
## K     0.0629815910  0.284019348 -0.241894819 -0.045950687  0.129705679
## Zn   -0.2111979853  0.144817667 -0.110451181  0.084128455  0.102662451
## Fe   -0.0943690234  0.125923593  0.347899730 -0.234668215 -0.006134716
## CEC  -0.0277757830 -0.353453286 -0.166879087  0.077098305  0.147528248
## GWC  -0.1045501499 -0.170436681  0.012319224 -0.386493578 -0.183303754
## BD    0.0955162297 -0.179231490  0.125514723  0.050766289  0.252582454
## POXC -0.0002862881  0.026435035  0.295544913  0.521721450 -0.011277482
## MWD  -0.1999298246  0.084321299 -0.018372408  0.018114554 -0.363920745
## Resp -0.2400311188  0.010103061 -0.005153055  0.147116748  0.215616549
## MB   -0.2570614607 -0.073250460  0.019162023 -0.114628399  0.104081288
## GmP  -0.2460119169 -0.063047337  0.072296229 -0.136936343  0.063965209
## GmN  -0.2447322393 -0.074853571 -0.043987196 -0.062242051  0.152726152
## Act  -0.1345037027 -0.131293582  0.201006055  0.139204451  0.206851906
## AMF  -0.2511261026 -0.123596334  0.013773325 -0.068338627  0.143507532
## Fun  -0.2166773591 -0.042447229  0.069750235 -0.179549759  0.036987921
## FB   -0.0822884448 -0.107862678 -0.115675735 -0.039695445  0.117772486
## bG   -0.1779859107  0.104165385 -0.069998950  0.283976708  0.185772527
## NAG  -0.2361438648  0.159873589  0.030984725  0.007738284 -0.034720848
## AP   -0.2316847097  0.146988125 -0.017564692  0.189741006 -0.112786853
## ALK  -0.2248120118 -0.047975178 -0.240021344  0.139894614 -0.105759756
## ARY  -0.2447724768 -0.018776633  0.011415478 -0.050892835 -0.296999066
## PHO  -0.2520702279 -0.077918350 -0.098382782 -0.001520702 -0.129340295
## Pro  -0.1718471784  0.245782651 -0.014008715 -0.054364001 -0.049632755
##              PC6           PC7         PC8          PC9         PC10
## Clay -0.02086106  0.2249699635 -0.21009163  0.331259031 -0.516289465
## TN   -0.08435706  0.0109950831 -0.13474356 -0.080982549  0.027997180
## SOC  -0.16938646  0.0397449871 -0.09670616 -0.089511588  0.035312627
## Ca   -0.07365511  0.0270973834 -0.08147922 -0.125476905  0.190114457
## Cu   -0.12145856  0.0478888510 -0.31638089  0.214415054 -0.116519789
## Mg   -0.25424489  0.1459502891  0.26346008 -0.113232526 -0.199468587
## Mn    0.10243693  0.1271499263 -0.04930112  0.312224900  0.072594994
## Na    0.05830157 -0.2645060503 -0.11291599 -0.319121396 -0.240414612
## P    -0.08630585  0.0146185885  0.03877671 -0.162243560  0.039857453
## pH    0.02722043  0.0071666956 -0.12148917 -0.060633430 -0.078717207
## K     0.03639169  0.2084125435  0.23194563 -0.062497014 -0.333505449
## Zn   -0.23046132  0.0463711960 -0.09730489 -0.240764162 -0.068868299
## Fe   -0.16754818 -0.1981937814 -0.20481211 -0.149098036  0.293566233
## CEC  -0.11906685  0.0752365501 -0.01694076 -0.169513015  0.127839528
## GWC  -0.35197487  0.2382982791  0.06664196 -0.115445937 -0.065532031
## BD   -0.32222048  0.3717230896  0.21257693  0.231072398  0.307620237
## POXC -0.04579081 -0.0000631283 -0.04155606 -0.063074441  0.184908770
## MWD   0.14632322  0.0173628609 -0.11649403  0.286262400  0.160566893
## Resp  0.03820870 -0.0045046522 -0.18039885  0.019229745 -0.089640187
## MB    0.16541124  0.0461810822  0.05835380 -0.044042818  0.080102806
## GmP   0.25595112  0.0364546545 -0.09140108 -0.029822350  0.037047724
## GmN   0.18883257  0.0532589174  0.09056703 -0.097919272  0.228110349
## Act   0.31476149  0.0613343958  0.41032009 -0.132556523 -0.200294445
## AMF   0.06088742 -0.1113799580  0.02221333  0.052895702  0.046518314
## Fun   0.19045558  0.0091604175  0.42856284 -0.001015258  0.138639626
## FB   -0.34626694 -0.6665167171  0.34438060  0.395331368 -0.062484600
## bG   -0.14497537 -0.2068712419 -0.01589960 -0.207306433 -0.230564054
## NAG  -0.08984146  0.0612139062  0.10474445  0.007452116 -0.041373675
## AP    0.07600283 -0.0290407901 -0.02718157  0.114284054 -0.031338745
## ALK  -0.06038836  0.0368442251  0.01169307  0.001417594 -0.008376931
## ARY  -0.03829034  0.0792409537  0.02500176  0.192900970 -0.031173282
## PHO  -0.06884595  0.0568151404 -0.02567387  0.125806768  0.058685607
## Pro  -0.27438533  0.1615709148  0.11542679 -0.088165998  0.007496480
##               PC11          PC12         PC13         PC14          PC15
## Clay  0.2148436711 -0.0007170844 -0.056337589  0.563005316 -0.0953808629
## TN    0.1784825601  0.2433401634 -0.061874462 -0.003800405  0.0480523789
## SOC   0.2019462498  0.1885442935 -0.068911552  0.009978064  0.0084512173
## Ca   -0.0072610776 -0.0207471235 -0.317779444 -0.017922301 -0.0449468859
## Cu   -0.1733122210 -0.2868989800 -0.144621971 -0.396445424 -0.1153460031
## Mg    0.1135836547 -0.0366550936  0.002822752 -0.161902714 -0.3907055397
## Mn    0.0543625088  0.0536206257 -0.201893661  0.034713505  0.0230329385
## Na   -0.3003005594  0.4769904484 -0.038038014  0.010033480 -0.1622304439
## P    -0.0228726096  0.0325334021 -0.014772434  0.190867261 -0.0343014364
## pH   -0.1668243743 -0.0398765147  0.200009783 -0.055949557 -0.0806889759
## K    -0.0534012079  0.1404419922  0.035176643 -0.257726117 -0.2582270225
## Zn    0.2811734700  0.1745665185  0.006166026 -0.091758221 -0.1325794900
## Fe    0.1236902032  0.0186232704 -0.052959659  0.262196185  0.0761522165
## CEC   0.0001173003 -0.0035930499 -0.341864980 -0.078492447 -0.1509134485
## GWC   0.1302998524 -0.2221531655  0.152710791 -0.239509788  0.4803355165
## BD   -0.3033947657  0.4523697861  0.324702554  0.125220384  0.0385724172
## POXC  0.0902560080 -0.0586433975 -0.024275695  0.061403470 -0.0658409544
## MWD  -0.2794252180  0.2023519272 -0.240141469 -0.124216298 -0.0371617727
## Resp  0.1610554991  0.2117899557  0.224487782 -0.184911942  0.1518368790
## MB    0.0016758284 -0.1097887288  0.151347427 -0.022704844 -0.0747778900
## GmP  -0.1330460836 -0.0228975710  0.165944480 -0.029192243 -0.0796940561
## GmN   0.0164336290 -0.1769207510  0.148939447  0.035275808 -0.1753159961
## Act  -0.0060801955  0.1496383960 -0.369648748 -0.017651456  0.4984083731
## AMF  -0.0540317360 -0.0411045339  0.120553604 -0.049080329 -0.0255166644
## Fun   0.2021574869 -0.1234325411  0.054017376  0.169845778 -0.2757288578
## FB    0.0853340757  0.0541419649 -0.039601702 -0.077672139 -0.0259126209
## bG   -0.4209002593 -0.2580897236  0.209418218  0.243584504  0.1233983355
## NAG  -0.1496121345 -0.0361799000 -0.035091954  0.105612071  0.0781809845
## AP    0.0723018340  0.0572721254  0.225023116 -0.005099303  0.1294133470
## ALK  -0.0863028863  0.0300438491 -0.105220740  0.048679927 -0.0146671479
## ARY  -0.1200470216 -0.0263183342 -0.061876138 -0.150249674 -0.0003755959
## PHO   0.0101753903 -0.0485894972 -0.048651105  0.025702540 -0.0669126173
## Pro  -0.3201760173 -0.1817604599 -0.290309158  0.214061766 -0.0312804902
##              PC16          PC17          PC18         PC19         PC20
## Clay -0.006498226 -0.0723282496  0.1347314093  0.003928476  0.061465724
## TN   -0.045579749  0.0769528480 -0.0859492784  0.206331897 -0.152707375
## SOC  -0.054601744  0.1108140448  0.0182371330  0.039434136 -0.215359152
## Ca   -0.163534717  0.0450767546  0.0256839220 -0.097887866 -0.088492137
## Cu    0.107417325 -0.0306139537  0.0666929494  0.369290069 -0.128914773
## Mg   -0.132620503  0.2507546394  0.0421858063 -0.238894513  0.213388384
## Mn   -0.059375509 -0.2238752272 -0.2629585113 -0.547178882 -0.066569018
## Na   -0.014744675 -0.2914926404 -0.1564194868 -0.040332133 -0.141326247
## P    -0.022349973 -0.0709891589  0.2019528947  0.257065996  0.505893631
## pH    0.022761432 -0.1659905027  0.0666011087  0.136986310  0.056880324
## K    -0.225876019 -0.2411008839  0.0255010434  0.012221099  0.002894847
## Zn    0.493882811  0.0467806412  0.0538421026 -0.042011718  0.055896691
## Fe   -0.124037455  0.0184616970  0.0377808182  0.002315550  0.125626695
## CEC  -0.228505985  0.0586063115  0.0355792167 -0.147432018 -0.061313381
## GWC  -0.017772550 -0.3308788653  0.1234336249 -0.162070409 -0.067314112
## BD    0.088115885  0.0487195985 -0.0280739296  0.044894035 -0.040828724
## POXC -0.049325884 -0.6592401157 -0.0046213296  0.052107362  0.162151114
## MWD   0.105094973 -0.0433042606  0.6107715147 -0.157986097  0.044355741
## Resp -0.108832546  0.0341796631  0.2807524815 -0.142776296  0.172317608
## MB    0.010734607  0.0080279396 -0.0345834584 -0.070437512  0.099790024
## GmP   0.003669583  0.1240842729 -0.0729396432  0.002581420  0.193195492
## GmN   0.016724589 -0.1488989769  0.0595999573 -0.032385385  0.111231079
## Act   0.139913311  0.0447173648  0.0008134444  0.116653313  0.173829953
## AMF  -0.124486472  0.0807414444 -0.0605631752 -0.110261075  0.224391453
## Fun   0.155817027 -0.1048152927  0.2098153729  0.118336607 -0.442185885
## FB    0.003085901 -0.0945555014  0.0482270095  0.020134297  0.100252345
## bG    0.095466001  0.0816724322  0.0730478450 -0.339299220 -0.167480466
## NAG  -0.623072814  0.0550865937  0.0903840377  0.215639594 -0.063538906
## AP   -0.164716376 -0.0002967628 -0.0903010076  0.190967976 -0.212036936
## ALK   0.087792439 -0.2213093897 -0.1722759178  0.102322758  0.009928299
## ARY   0.122871636  0.0671335429 -0.3148531710  0.062108895  0.124386632
## PHO   0.015097932 -0.0105647720 -0.3789654983  0.040903403  0.217779433
## Pro   0.176231452  0.0084390474  0.0090856022  0.081646661  0.017225557
##              PC21         PC22         PC23         PC24         PC25
## Clay  0.015938733  0.036138675 -0.134824579 -0.002859878 -0.052965316
## TN    0.123210724 -0.342910365 -0.331353124 -0.078432422  0.102340993
## SOC   0.072878219 -0.175831179 -0.091479527 -0.055373142  0.088582377
## Ca   -0.024595171  0.052506488  0.003593264  0.007975998  0.004593207
## Cu   -0.124521127  0.027129207  0.003891066  0.076960138  0.065965606
## Mg    0.006142689 -0.008582279  0.019166633  0.129052200  0.074851057
## Mn    0.076201169  0.059114136  0.157218236 -0.110998608  0.125935407
## Na    0.092348138  0.094441916  0.090086647  0.151635101  0.125479283
## P    -0.088554459 -0.021804243  0.004326672  0.159293883  0.403072121
## pH    0.025764925 -0.020617610  0.345139838 -0.440077007 -0.132206842
## K    -0.285462763 -0.121173578 -0.188515380 -0.156101147 -0.386071677
## Zn   -0.029694591  0.466363127  0.047576539 -0.266082655  0.064776827
## Fe   -0.402862418 -0.075291328 -0.001348548 -0.147803809 -0.451390961
## CEC  -0.058334698  0.042791275 -0.013780046  0.015129884 -0.026081162
## GWC   0.002541101  0.007584349 -0.105450359  0.007364000  0.060819950
## BD   -0.063193000  0.021482991 -0.042764762 -0.010500805 -0.012343023
## POXC  0.094599539 -0.092760921 -0.064441565 -0.165772475  0.142148351
## MWD  -0.158494979  0.078134454 -0.132264590 -0.020917237  0.044454235
## Resp  0.118978952 -0.323747276  0.485875066  0.288792295 -0.104791783
## MB    0.042002129  0.048470303 -0.109332754  0.009695655 -0.032832993
## GmP   0.191076223 -0.165970655 -0.156273624 -0.283360121 -0.026258099
## GmN   0.170256933  0.206355269 -0.216578816  0.296797978 -0.129239278
## Act  -0.112497647  0.048971615  0.016302798 -0.089318466 -0.053234582
## AMF   0.034578189  0.110068074 -0.166828007 -0.133225407 -0.015436819
## Fun  -0.168476923 -0.210342287  0.246810642 -0.049204453  0.162366658
## FB    0.130746517  0.002181195 -0.059238963 -0.058086110 -0.079427578
## bG   -0.275869223 -0.120814426 -0.121046101 -0.011777668  0.167040430
## NAG   0.080448809  0.249563901  0.138158106 -0.191791425  0.255666156
## AP   -0.041213694  0.456157996  0.031454518  0.182788943 -0.163037504
## ALK  -0.130769871 -0.076913405 -0.116264201  0.449753936 -0.132759830
## ARY  -0.016557533 -0.218567620 -0.078052559 -0.093838101  0.164670300
## PHO  -0.443418570  0.005956114  0.338980636  0.057516427  0.100298497
## Pro   0.463795458 -0.060574977  0.239951616  0.018061249 -0.365769777
##              PC26         PC27         PC28         PC29          PC30
## Clay -0.045463114 -0.056440075  0.034940293  0.029152497  0.0006269166
## TN   -0.092094013  0.366333020  0.209772608 -0.307316691 -0.1048106381
## SOC  -0.089220722 -0.083384976 -0.109219233  0.073970735  0.1659162449
## Ca   -0.111648143 -0.044335739 -0.047275203  0.161180087 -0.0308590277
## Cu    0.032306176  0.089562083 -0.006209569 -0.008718812  0.0104324520
## Mg    0.066617829  0.373157801 -0.035928601  0.012544353 -0.0480295701
## Mn    0.120133083  0.308431053  0.018121220  0.077348209 -0.0161272177
## Na   -0.082789661 -0.022593699  0.030287907 -0.015310944  0.0151646559
## P    -0.139471045  0.217582718 -0.108332018  0.211477279  0.0627166455
## pH    0.152639977  0.467815973  0.148966899  0.035142377  0.0887582800
## K    -0.122641403 -0.169034394  0.030190688  0.017605756  0.0063260192
## Zn    0.170834824 -0.181480784  0.020842473  0.026464333 -0.0485418985
## Fe    0.136452049  0.088022490  0.091467111  0.159651253  0.0244570571
## CEC  -0.126125587 -0.003936428 -0.053553720  0.179471281 -0.0409292064
## GWC  -0.062210651  0.040989655 -0.066508131 -0.052249059 -0.0856296135
## BD    0.013216980 -0.007668813  0.008943808  0.017071491 -0.0166957993
## POXC -0.125959830 -0.114833541 -0.099578524  0.038438435  0.0367748997
## MWD  -0.070139865  0.040793879 -0.014848178 -0.094861599 -0.0350353137
## Resp  0.010865663 -0.168443969  0.065835389  0.078081316  0.0290399496
## MB    0.007042866 -0.131867181 -0.204684432  0.051392453  0.0962968230
## GmP   0.021790928 -0.023091936 -0.395952720  0.136218299 -0.5374083753
## GmN  -0.016318689  0.030728004  0.601386959 -0.035564508 -0.1473156811
## Act   0.010117994  0.123244995  0.064767927  0.013285761 -0.0117640805
## AMF  -0.055914758  0.025215984 -0.183735342 -0.341377171  0.6613642743
## Fun   0.003046567  0.030045884 -0.089735459  0.042038492  0.0800575106
## FB   -0.016610407 -0.005786716  0.001192895  0.047018675 -0.1788472176
## bG   -0.073484563  0.026739995  0.069459104  0.066005974 -0.0440436751
## NAG   0.354178103 -0.222994374  0.130875747 -0.075341094 -0.0937985072
## AP   -0.374949416  0.304062327 -0.205386983  0.326919980  0.0007224411
## ALK   0.614244699  0.090788230 -0.293042048 -0.007787326  0.0213150158
## ARY  -0.010836076 -0.170783345  0.331131282  0.556306315  0.2439453717
## PHO  -0.312187662 -0.118722689  0.026739128 -0.406688440 -0.2441427152
## Pro  -0.212597242 -0.018200644 -0.076375756 -0.086877488  0.0639173242
##               PC31         PC32          PC33
## Clay  3.105705e-02  0.009614304  0.000000e+00
## TN    3.045723e-01  0.230178127 -5.073813e-16
## SOC  -7.539875e-01 -0.212481381  4.557855e-16
## Ca    1.311742e-01  0.008826359  7.289232e-01
## Cu   -5.832655e-02 -0.005155952  3.654456e-17
## Mg   -6.942920e-02  0.009886825  1.166931e-01
## Mn   -3.540812e-02  0.005467538 -1.295359e-16
## Na    1.715675e-02  0.017633904  1.195006e-02
## P     1.138698e-05 -0.006321490  1.537687e-16
## pH   -1.907801e-01  0.060618705  4.992630e-16
## K     1.942732e-02 -0.022454242  8.041946e-02
## Zn    1.620812e-01 -0.013579660  2.450434e-16
## Fe    2.811088e-02  0.016360875 -1.438994e-17
## CEC   1.333236e-01  0.008948476 -6.696593e-01
## GWC   6.492429e-02 -0.037402194 -5.565015e-17
## BD   -1.317832e-03  0.008892750  2.932853e-16
## POXC -7.943478e-03 -0.006778343  5.931477e-17
## MWD  -2.987538e-02  0.036804940 -8.981211e-17
## Resp  1.487368e-01 -0.005595816 -2.773646e-16
## MB   -1.876606e-01  0.825766289  1.935370e-15
## GmP   6.328646e-02 -0.283432311 -7.122570e-16
## GmN  -1.685836e-01 -0.185363418 -1.684147e-16
## Act  -1.176610e-01 -0.015624714  1.111357e-16
## AMF   1.895246e-01 -0.278802639 -1.980288e-16
## Fun   1.834686e-01 -0.087682985 -2.879613e-16
## FB   -5.029521e-02  0.047810433  3.636857e-17
## bG   -9.432641e-03 -0.004269481  4.836532e-17
## NAG   4.025449e-02  0.036867836  2.902767e-16
## AP    8.840100e-02 -0.031226506 -2.399837e-16
## ALK   1.952869e-02 -0.076761614  2.690181e-16
## ARY   1.563570e-01 -0.015876602  8.614383e-17
## PHO  -1.040811e-01 -0.004413650  6.158793e-17
## Pro   5.576237e-02  0.022897327 -1.998972e-16
## Importance of components:
##                          PC1    PC2    PC3     PC4     PC5     PC6     PC7
## Standard deviation     3.615 2.4186 1.9451 1.44016 1.21782 1.05390 0.96599
## Proportion of Variance 0.396 0.1773 0.1147 0.06285 0.04494 0.03366 0.02828
## Cumulative Proportion  0.396 0.5733 0.6880 0.75081 0.79575 0.82941 0.85768
##                            PC8     PC9   PC10    PC11    PC12    PC13    PC14
## Standard deviation     0.91349 0.83594 0.7579 0.65345 0.61808 0.57860 0.51838
## Proportion of Variance 0.02529 0.02118 0.0174 0.01294 0.01158 0.01014 0.00814
## Cumulative Proportion  0.88297 0.90415 0.9215 0.93449 0.94607 0.95621 0.96435
##                           PC15    PC16    PC17   PC18    PC19    PC20    PC21
## Standard deviation     0.46416 0.38426 0.37432 0.3631 0.34492 0.27043 0.25505
## Proportion of Variance 0.00653 0.00447 0.00425 0.0040 0.00361 0.00222 0.00197
## Cumulative Proportion  0.97088 0.97536 0.97960 0.9836 0.98720 0.98942 0.99139
##                           PC22    PC23    PC24   PC25    PC26    PC27    PC28
## Standard deviation     0.23595 0.21771 0.20008 0.1814 0.17629 0.15902 0.13542
## Proportion of Variance 0.00169 0.00144 0.00121 0.0010 0.00094 0.00077 0.00056
## Cumulative Proportion  0.99308 0.99451 0.99573 0.9967 0.99767 0.99843 0.99899
##                           PC29    PC30    PC31    PC32      PC33
## Standard deviation     0.12348 0.09618 0.07228 0.06080 3.665e-16
## Proportion of Variance 0.00046 0.00028 0.00016 0.00011 0.000e+00
## Cumulative Proportion  0.99945 0.99973 0.99989 1.00000 1.000e+00

2.5 Less variables- biologicals

##  [1] "sample_name" "location"    "treatment"   "Resp"        "MB"         
##  [6] "GmP"         "GmN"         "Act"         "AMF"         "Fun"        
## [11] "bG"          "NAG"         "AP"          "ALK"         "ARY"        
## [16] "PHO"         "Pro"

2.6 Less PCA- subset by location

## Standard deviations (1, .., p=14):
##  [1] 3.08173598 1.26228497 0.89723179 0.84222540 0.63866923 0.58132238
##  [7] 0.46003241 0.38379076 0.31323615 0.28387328 0.21655061 0.17174994
## [13] 0.16442586 0.09101219
## 
## Rotation (n x k) = (14 x 14):
##             PC1         PC2         PC3         PC4         PC5          PC6
## Resp -0.2793812 -0.02486202  0.29051594 -0.28115029  0.13807424 -0.436831954
## MB   -0.3070801  0.21193467 -0.05564918  0.03501593  0.22506875  0.009755181
## GmP  -0.2924982  0.22473237 -0.05090488  0.07293425  0.23859381 -0.232977931
## GmN  -0.2961093  0.19986562 -0.04172352 -0.05039611  0.32571499  0.197766168
## Act  -0.1695054  0.42092409  0.56344153  0.19006048 -0.60617258  0.144763382
## AMF  -0.2977739  0.22626088 -0.01381352 -0.14189101  0.18095745  0.013475682
## Fun  -0.2620103  0.26827121 -0.09446396  0.40347819  0.09735191  0.226177698
## bG   -0.2121104 -0.35608128  0.51992011 -0.26956760  0.18459567  0.359038982
## NAG  -0.2750684 -0.29361030  0.07504938  0.33065864 -0.01857088 -0.177290495
## AP   -0.2700601 -0.29099381  0.10370681 -0.01456582 -0.18951969 -0.474585804
## ALK  -0.2647955 -0.15359245 -0.20782987 -0.44625536 -0.27951091  0.387877313
## ARY  -0.2822555 -0.05921448 -0.37343307  0.07289287 -0.39500400 -0.149933024
## PHO  -0.2939780 -0.01309293 -0.33451355 -0.22413873 -0.21623487  0.091431030
## Pro  -0.1963859 -0.48859743 -0.01848312  0.50587464  0.07964877  0.276653527
##              PC7         PC8         PC9         PC10        PC11         PC12
## Resp  0.05802612 -0.50207543  0.34790201 -0.351590012  0.15925803 -0.061608687
## MB   -0.06116865 -0.01256922 -0.12100260  0.005908766 -0.01587731  0.034106631
## GmP  -0.35608388  0.09462334 -0.44142737 -0.066552970  0.33442029  0.429356900
## GmN   0.23056691 -0.16212547 -0.36988259  0.128072051 -0.31764292 -0.321196687
## Act  -0.09919723 -0.14118411 -0.10613837  0.035092585 -0.09427158  0.029892303
## AMF  -0.25957767  0.09787748  0.33338318  0.279419700 -0.23712521 -0.346637165
## Fun   0.52555960  0.24640191  0.35691640 -0.283710594  0.24066839  0.086029795
## bG   -0.16449165  0.49267293  0.05857575 -0.125686893  0.06286241  0.042674857
## NAG   0.02290150 -0.04545760  0.21050241  0.719901685  0.25228676  0.064512208
## AP    0.45619381  0.28499901 -0.31771602 -0.046711050 -0.31575457 -0.005930972
## ALK   0.23844920 -0.25834481 -0.20308811  0.124370374  0.43245097 -0.024012408
## ARY  -0.33722368  0.26901402  0.02099782 -0.262163774  0.15982130 -0.478858765
## PHO  -0.08777183 -0.01356980  0.28984167 -0.002251208 -0.46678579  0.585470726
## Pro  -0.21336466 -0.39621334 -0.09204655 -0.274369799 -0.20215628 -0.019950428
##              PC13         PC14
## Resp  0.130119564 -0.042525269
## MB    0.018469231  0.886216020
## GmP  -0.132189017 -0.310046461
## GmN   0.457918630 -0.276542127
## Act   0.004135394 -0.005672800
## AMF  -0.584588501 -0.150252906
## Fun  -0.067275666 -0.111234556
## bG    0.165651742  0.004624773
## NAG   0.241642293 -0.004902870
## AP   -0.277880322  0.029278569
## ALK  -0.259353700  0.014588455
## ARY   0.288767540 -0.007575280
## PHO   0.200345391 -0.058705413
## Pro  -0.235455445 -0.024657132
## Importance of components:
##                           PC1    PC2    PC3     PC4     PC5     PC6     PC7
## Standard deviation     3.0817 1.2623 0.8972 0.84223 0.63867 0.58132 0.46003
## Proportion of Variance 0.6784 0.1138 0.0575 0.05067 0.02914 0.02414 0.01512
## Cumulative Proportion  0.6784 0.7922 0.8497 0.90034 0.92948 0.95362 0.96874
##                            PC8     PC9    PC10    PC11    PC12    PC13    PC14
## Standard deviation     0.38379 0.31324 0.28387 0.21655 0.17175 0.16443 0.09101
## Proportion of Variance 0.01052 0.00701 0.00576 0.00335 0.00211 0.00193 0.00059
## Cumulative Proportion  0.97926 0.98626 0.99202 0.99537 0.99748 0.99941 1.00000

2.7 Less PCA-subset by land use

## Standard deviations (1, .., p=14):
##  [1] 3.08173598 1.26228497 0.89723179 0.84222540 0.63866923 0.58132238
##  [7] 0.46003241 0.38379076 0.31323615 0.28387328 0.21655061 0.17174994
## [13] 0.16442586 0.09101219
## 
## Rotation (n x k) = (14 x 14):
##             PC1         PC2         PC3         PC4         PC5          PC6
## Resp -0.2793812 -0.02486202  0.29051594 -0.28115029  0.13807424 -0.436831954
## MB   -0.3070801  0.21193467 -0.05564918  0.03501593  0.22506875  0.009755181
## GmP  -0.2924982  0.22473237 -0.05090488  0.07293425  0.23859381 -0.232977931
## GmN  -0.2961093  0.19986562 -0.04172352 -0.05039611  0.32571499  0.197766168
## Act  -0.1695054  0.42092409  0.56344153  0.19006048 -0.60617258  0.144763382
## AMF  -0.2977739  0.22626088 -0.01381352 -0.14189101  0.18095745  0.013475682
## Fun  -0.2620103  0.26827121 -0.09446396  0.40347819  0.09735191  0.226177698
## bG   -0.2121104 -0.35608128  0.51992011 -0.26956760  0.18459567  0.359038982
## NAG  -0.2750684 -0.29361030  0.07504938  0.33065864 -0.01857088 -0.177290495
## AP   -0.2700601 -0.29099381  0.10370681 -0.01456582 -0.18951969 -0.474585804
## ALK  -0.2647955 -0.15359245 -0.20782987 -0.44625536 -0.27951091  0.387877313
## ARY  -0.2822555 -0.05921448 -0.37343307  0.07289287 -0.39500400 -0.149933024
## PHO  -0.2939780 -0.01309293 -0.33451355 -0.22413873 -0.21623487  0.091431030
## Pro  -0.1963859 -0.48859743 -0.01848312  0.50587464  0.07964877  0.276653527
##              PC7         PC8         PC9         PC10        PC11         PC12
## Resp  0.05802612 -0.50207543  0.34790201 -0.351590012  0.15925803 -0.061608687
## MB   -0.06116865 -0.01256922 -0.12100260  0.005908766 -0.01587731  0.034106631
## GmP  -0.35608388  0.09462334 -0.44142737 -0.066552970  0.33442029  0.429356900
## GmN   0.23056691 -0.16212547 -0.36988259  0.128072051 -0.31764292 -0.321196687
## Act  -0.09919723 -0.14118411 -0.10613837  0.035092585 -0.09427158  0.029892303
## AMF  -0.25957767  0.09787748  0.33338318  0.279419700 -0.23712521 -0.346637165
## Fun   0.52555960  0.24640191  0.35691640 -0.283710594  0.24066839  0.086029795
## bG   -0.16449165  0.49267293  0.05857575 -0.125686893  0.06286241  0.042674857
## NAG   0.02290150 -0.04545760  0.21050241  0.719901685  0.25228676  0.064512208
## AP    0.45619381  0.28499901 -0.31771602 -0.046711050 -0.31575457 -0.005930972
## ALK   0.23844920 -0.25834481 -0.20308811  0.124370374  0.43245097 -0.024012408
## ARY  -0.33722368  0.26901402  0.02099782 -0.262163774  0.15982130 -0.478858765
## PHO  -0.08777183 -0.01356980  0.28984167 -0.002251208 -0.46678579  0.585470726
## Pro  -0.21336466 -0.39621334 -0.09204655 -0.274369799 -0.20215628 -0.019950428
##              PC13         PC14
## Resp  0.130119564 -0.042525269
## MB    0.018469231  0.886216020
## GmP  -0.132189017 -0.310046461
## GmN   0.457918630 -0.276542127
## Act   0.004135394 -0.005672800
## AMF  -0.584588501 -0.150252906
## Fun  -0.067275666 -0.111234556
## bG    0.165651742  0.004624773
## NAG   0.241642293 -0.004902870
## AP   -0.277880322  0.029278569
## ALK  -0.259353700  0.014588455
## ARY   0.288767540 -0.007575280
## PHO   0.200345391 -0.058705413
## Pro  -0.235455445 -0.024657132
## Importance of components:
##                           PC1    PC2    PC3     PC4     PC5     PC6     PC7
## Standard deviation     3.0817 1.2623 0.8972 0.84223 0.63867 0.58132 0.46003
## Proportion of Variance 0.6784 0.1138 0.0575 0.05067 0.02914 0.02414 0.01512
## Cumulative Proportion  0.6784 0.7922 0.8497 0.90034 0.92948 0.95362 0.96874
##                            PC8     PC9    PC10    PC11    PC12    PC13    PC14
## Standard deviation     0.38379 0.31324 0.28387 0.21655 0.17175 0.16443 0.09101
## Proportion of Variance 0.01052 0.00701 0.00576 0.00335 0.00211 0.00193 0.00059
## Cumulative Proportion  0.97926 0.98626 0.99202 0.99537 0.99748 0.99941 1.00000

2.8 Less PCA- subset by land use with C and N

## Standard deviations (1, .., p=16):
##  [1] 3.33015629 1.32745203 0.90153038 0.84911178 0.64259303 0.60224871
##  [7] 0.50569455 0.44735680 0.32909163 0.28970514 0.25943310 0.21410430
## [13] 0.17117855 0.15870662 0.12964562 0.07761118
## 
## Rotation (n x k) = (16 x 16):
##             PC1          PC2          PC3         PC4         PC5         PC6
## TN   -0.2677758 -0.245511420 -0.035984542  0.11486974 -0.08034124  0.27191938
## SOC  -0.2778839 -0.175690702  0.092215419  0.05731012 -0.12581784  0.17039065
## Resp -0.2621482 -0.006616583 -0.286043783  0.28865180 -0.19664248  0.39253376
## MB   -0.2787346  0.244726896  0.054593969 -0.03447562 -0.21015745 -0.06729087
## GmP  -0.2646693  0.256122777  0.048081881 -0.06920994 -0.23914893  0.11055132
## GmN  -0.2691429  0.229232236  0.040651121  0.05022275 -0.29144644 -0.22534061
## Act  -0.1453383  0.434083090 -0.561489078 -0.16582107  0.57227462  0.04561454
## AMF  -0.2700299  0.254780886  0.015202865  0.13884947 -0.15614581 -0.09772044
## Fun  -0.2349928  0.299957333  0.091587624 -0.39277905 -0.09361773 -0.14853814
## bG   -0.2008270 -0.293766067 -0.527099730  0.21547953 -0.07131728 -0.52944152
## NAG  -0.2591588 -0.229786056 -0.086758937 -0.35835147  0.01677500  0.11535290
## AP   -0.2555415 -0.238128951 -0.116762920 -0.01264269  0.16814108  0.38223849
## ALK  -0.2491785 -0.118248178  0.201472379  0.40913026  0.34459970 -0.32723379
## ARY  -0.2602273 -0.006695749  0.360622025 -0.10573570  0.41673756  0.09026262
## PHO  -0.2721212  0.025494076  0.328469956  0.19624896  0.25422981 -0.10737239
## Pro  -0.1904176 -0.420686128  0.004404824 -0.54640665 -0.03758373 -0.27430553
##              PC7         PC8         PC9          PC10         PC11
## TN   -0.36182823  0.10593697 -0.06489674  0.2118618281 -0.426767457
## SOC  -0.35154424  0.24522111  0.26405736  0.0866283093 -0.218623768
## Resp -0.02588831  0.13698799  0.12706575 -0.0001835356  0.664384348
## MB    0.07636513  0.02499022 -0.11971026  0.0044953926  0.007581183
## GmP   0.37094180  0.18198681 -0.35410726  0.2316691090 -0.266728760
## GmN  -0.15363761 -0.17587457 -0.43742049 -0.1501643266  0.135700728
## Act  -0.20036696  0.21649539 -0.10412713 -0.0046696974 -0.056673318
## AMF   0.19971748  0.17008457  0.30283673 -0.2682836728 -0.131969935
## Fun  -0.32534158 -0.47490628  0.41558524  0.2437686947  0.062477034
## bG    0.29968464 -0.10533100  0.22910980  0.2297940023 -0.137731135
## NAG   0.13119308 -0.05836503  0.09671768 -0.7365729382 -0.180141501
## AP    0.19735073 -0.62210313 -0.21330820  0.0893091662  0.026832827
## ALK  -0.28625289 -0.09753654 -0.28993608 -0.1525323786 -0.008113886
## ARY   0.39065245  0.11795360  0.11361138  0.2919656757  0.039830971
## PHO   0.06106878  0.06786213  0.22088141 -0.1036852223  0.203547769
## Pro  -0.08753516  0.33811624 -0.22469654  0.1317840478  0.345389721
##              PC12          PC13         PC14        PC15         PC16
## TN    0.123658800 -0.0169094659 -0.091625268 -0.54069008  0.288872393
## SOC   0.040358923 -0.0319272587 -0.098193093  0.64026120 -0.322945399
## Resp -0.285799310 -0.0004515216 -0.011209781 -0.12058376 -0.002617374
## MB    0.005787031  0.0361190200 -0.006316851  0.40134246  0.791349700
## GmP  -0.270869775  0.3927065166  0.195612073 -0.05538442 -0.313035908
## GmN   0.246296496 -0.2290411805 -0.508046781 -0.07163455 -0.268176234
## Act   0.107396613  0.0224178686 -0.024717784  0.05775299 -0.032044025
## AMF   0.284294228 -0.4569772153  0.483485099 -0.16649331 -0.087647491
## Fun  -0.228012675  0.0790507802  0.101145447 -0.14992087 -0.054278809
## bG   -0.041171873  0.0670567959 -0.178170562  0.02393785 -0.005340188
## NAG  -0.267350592  0.1198841263 -0.188398809 -0.06152802  0.020324443
## AP    0.314444054 -0.0660004931  0.257520752  0.19155540 -0.058518336
## ALK  -0.438941921 -0.0576013566  0.306604665  0.03528823 -0.005407372
## ARY  -0.188362303 -0.4119725799 -0.366072008 -0.05777331  0.019556671
## PHO   0.444539531  0.6131664160 -0.083667092 -0.11285321 -0.010112074
## Pro   0.158660695 -0.0538018658  0.268796248 -0.04474286 -0.008531989
## Importance of components:
##                           PC1    PC2    PC3     PC4     PC5     PC6     PC7
## Standard deviation     3.3302 1.3275 0.9015 0.84911 0.64259 0.60225 0.50569
## Proportion of Variance 0.6931 0.1101 0.0508 0.04506 0.02581 0.02267 0.01598
## Cumulative Proportion  0.6931 0.8033 0.8540 0.89911 0.92492 0.94759 0.96357
##                            PC8     PC9    PC10    PC11    PC12    PC13    PC14
## Standard deviation     0.44736 0.32909 0.28971 0.25943 0.21410 0.17118 0.15871
## Proportion of Variance 0.01251 0.00677 0.00525 0.00421 0.00287 0.00183 0.00157
## Cumulative Proportion  0.97608 0.98285 0.98810 0.99230 0.99517 0.99700 0.99857
##                           PC15    PC16
## Standard deviation     0.12965 0.07761
## Proportion of Variance 0.00105 0.00038
## Cumulative Proportion  0.99962 1.00000