Mixed ANOVA + Pairwise Comparison (CL data)

Description

Source of motivation: Mixed ANOVA in R

Assumptions Check

• Outliers. Values above Q3 + 1.5xIQR or below Q1 - 1.5xIQR are considered as outliers. Values above Q3 + 3xIQR or below Q1 - 3xIQR are considered as extreme points (or extreme outliers). Observations that are considered as extreme points are marked red.

• Normality Assumptions. Plot shows Q-Q plot for subsets of data. If points lie on the line, we can assume that data is fairly symmetrical (and that’s what we want).

• Homogeneity of Variance Assumption. Performed using Levene’s test.

  • \(H_0\): the groups have equal population variances
  • \(H_A\): the groups don’t have equal population variances
  • Observations with p-value < 0.05 are marked red, since they may violate the ANOVA results.

Mixed ANOVA

“In statistics, a mixed-design analysis of variance model, also known as a split-plot ANOVA, is used to test for differences between two or more independent groups whilst subjecting participants to repeated measures.” (Wikipedia)

• within subjects group (with repeated measures): Measurement
• between subjects group: Group

Post-hoc Tests

• Significant two-way interaction. Can be used if an interaction term in Mixed ANOVA results showed significance. In other words, impact of Group on the outcome variable (PeakAmp, PeakLat, RMS) depends on the Measurement (and vice versa)

  • Pairwise comparisons between Group. Performs Welch’s t-tests (for unequal variances) for comparing Group for each Measurement

  • Pairwise comparisons between Measurement. Performs paired two-sample t-test for comparing Measurement for each Group.

• Non-significant two-way interaction. Shows the main effect for Group and Measurement. Used if there was non-significant two-way interaction.

  For each table observations with adjusted p-value (using Holm–Bonferroni method) which is less then 0.05 are marked green.

library(tidyverse)
library(ggpubr)
library(rstatix)
library(knitr)
library(kableExtra)

data_type <- "CLST"
raw_data <- read_csv(paste0("../../Data_new/AVRECPeak", data_type,".csv"))

layers <- unique(raw_data$Layer)
stimfreq <- unique(raw_data$ClickFreq)
metrics <- c("PeakAmp", "PeakLat", "RMS", "logRMS")
stimfreq <- c(5, 10)


red_color <- "#ffb3b3"
green_color <- "#d6f5d6"

# values for testing
# metrics <- c("RMS", "logRMS")
freq <- 5
layer <- "All"
metric <- "logRMS"

Results

for (freq in stimfreq){

  cat("##", freq, "Hz Frequency {.tabset .tabset-fade .tabset-pills} \n")
  
  for (layer in layers){
  # for (layer in c("All", "I_II")){
    cat("###", layer, " Layer {.tabset .tabset-fade .tabset-pills}\n")
    
    model_data <- raw_data %>% 
      filter(
        ClickFreq == freq,
        Layer == layer,
        OrderofClick == 1,
        grepl('_1', Measurement),
        PeakAmp > 0) %>% 
      mutate(
        logRMS = log(RMS),
        Measurement = case_when(
          grepl('pre', Measurement) ~ "preLaser",
          TRUE ~ "postLaser"
        )
      ) %>% 
      select(-c(Layer, ClickFreq, OrderofClick))
  
    # get average by Trial
    if(grepl("ST", data_type)){
      model_data <- model_data %>%
        group_by(Group, Animal, Measurement) %>%
        summarize(
          PeakAmp = mean(PeakAmp, na.rm = TRUE),
          PeakLat = mean(PeakLat, na.rm = TRUE),
          RMS = mean(RMS, na.rm = TRUE),
          logRMS = mean(logRMS, na.rm = TRUE)) %>%
        ungroup() %>% 
        convert_as_factor(Group, Animal, Measurement) 
    }
    
    # if(grepl("ST", data_type)){
    #   model_data <- model_data %>% 
    #     mutate(Animal = paste0(Animal, "_", TrialNumber)) %>% 
    #     select(-TrialNumber) %>% 
    #     ungroup()
    # }
  
    # exclude_animals <- model_data %>%
    #   group_by(Animal, Layer) %>%
    #   summarise(n = n_distinct(Measurement)) %>%
    #   filter(n < 2) %>%
    #   pull(Animal)
    # 
    # model_data <- model_data %>% 
    #   filter(!(Animal %in% exclude_animals)) %>%
    #   select(Group, Animal, Measurement, Layer, PeakAmp, PeakLat, RMS, logRMS) %>% 
    #   convert_as_factor(Group, Animal, Measurement, Layer) 
    
      for (metric in metrics){
      # for (metric in c("PeakLat")){

        cat("####", metric, "{.tabset .tabset-fade .tabset-pills}\n")
        
        cat("##### Assumptions Check\n")
        
        cat("<b>===OUTLIERS===</b>\n\n")
        
        df <- model_data %>%
          group_by(Measurement, Group) %>%
          identify_outliers(eval(metric))
        
        if(nrow(df) > 0){
          df %>% 
          kable(escape = F, digits = 6) %>%
          kable_classic_2(full_width = F) %>% 
          kable_styling() %>% 
          row_spec(
            which(df$is.extreme == TRUE), bold = T, 
            background = red_color) %>% 
          print()
        } else{
          cat("\n\nNo outliers were detected.\n\n")
        }
        
        cat("\n\n<b>===NORMALITY ASSUMPTION===</b>\n\n")
        
        p <- ggqqplot(model_data, metric, ggtheme = theme_bw()) +
          facet_grid(Group ~ Measurement, labeller = "label_both")
        plot(p)

        cat("\n\n<b>===HOMOGENEITY OF VARIANCE ASSUMPTION===</b>\n\n")
        
        df <- model_data %>%
          group_by(Measurement) %>%
          levene_test(formula = as.formula(paste0(metric, "~ Group"))) 
        
        df %>% 
          kable(escape = F, digits = 3) %>%
          kable_classic_2(full_width = F) %>% 
          kable_styling() %>% 
          row_spec(
            which(df$p <= 0.05), bold = T, 
            background = red_color) %>% 
          print()
        
        cat('\n\n')

        cat("##### Mixed ANOVA\n")

        # Two-way mixed ANOVA test
        res.aov <- anova_test(
          data = model_data, dv = eval(metric), wid = Animal,
          between = Group, within = c(Measurement))
        
        df <- get_anova_table(res.aov) %>%
          data.frame()
        
        df %>% 
          kable(escape = F,
                caption = "Mixed ANOVA (Type III test)") %>%
          kable_classic_2(full_width = F) %>% 
          kable_styling() %>% 
          row_spec(
            which(df$p <= 0.05), bold = T, 
            background = green_color) %>% 
          print()

        cat('\n\n')

        cat("##### Post-hoc Tests\n")

        cat("\n\n<b>===SIGNIFICANT TWO-WAY INTERACTION===</b>\n\n")
        
        # cat("\n\n\n<b>=EFFECT OF GROUP ON MEASUREMENT=</b>\n\n")
        
        # one.way <- model_data %>%
        #   group_by(Group) %>%
        #   anova_test(dv = eval(metric), wid = Animal, within = c(Measurement)) %>% 
        #   get_anova_table() %>%
        #   adjust_pvalue(method = "holm")
  
        # one.way <- model_data %>%
        #   group_by(Measurement) %>%
        #   anova_test(dv = eval(metric), wid = Animal, between = Group) %>%
        #   get_anova_table() %>%
        #   adjust_pvalue(method = "bonferroni")
        
        # df <- one.way %>% 
        #   data.frame() %>% 
        #   select(-F)
        # df %>% 
        #   kable(escape = F, digits = 4) %>%
        #   kable_classic_2(full_width = F) %>% 
        #   kable_styling() %>% 
        #   row_spec(
        #     which(df$p.adj <= 0.05), bold = T, 
        #     background = green_color) %>% 
        #   print()
        
        cat("\n\n\n<b>=PAIRWISE COMPARISONS BETWEEN GROUP LEVELS=</b>\n\n")
        
        pwc <- model_data %>%
          group_by(Measurement) %>%
          pairwise_t_test(
            as.formula(paste0(metric, "~ Group")),
            p.adjust.method = "holm",
            pool.sd = FALSE,
            var.equal = FALSE)

        eff_size <- model_data %>%
          group_by(Measurement) %>%
          cohens_d(
            as.formula(paste0(metric, "~ Group")),
            var.equal = FALSE)
        
        df <- pwc %>% 
          left_join(eff_size) %>% 
          data.frame() 
        
        df %>% 
          kable(escape = F, digits = 3) %>%
          kable_classic_2(full_width = F) %>% 
          kable_styling() %>% 
          row_spec(
            which(df$p.adj <= 0.05), bold = T, 
            background = green_color) %>% 
          print()
        
        # cat("\n\n\n<b>=EFFECT OF MEASUREMENT AT EACH LEVEL OF GROUP=</b>\n\n")
        # 
        # one.way2 <- model_data %>%
        #   group_by(Measurement) %>%
        #   anova_test(dv = eval(metric), wid = Animal, between = Group) %>%
        #   get_anova_table() %>%
        #   adjust_pvalue(method = "holm")
        # 
        # df <- one.way2 %>%
        #   data.frame()
        # df %>% 
        #   kable(escape = F) %>%
        #   kable_classic_2(full_width = F) %>% 
        #   kable_styling() %>% 
        #   row_spec(
        #     which(df$p.adj <= 0.05), bold = T, 
        #     background = green_color) %>% 
        #   print()
        
        cat("\n\n\n<b>=PAIRWISE COMPARISONS BETWEEN MEASUREMENTS AT EACH GROUP LEVELS=</b>\n\n")
        
        # exclude_animals <- model_data %>%
        #   group_by(Animal) %>%
        #   summarise(n = n_distinct(Measurement)) %>%
        #   filter(n < 2) %>%
        #   pull(Animal)
        
        pwc2 <- model_data %>%
          # filter(!(Animal %in% exclude_animals)) %>%
          group_by(Group) %>%
          pairwise_t_test(
            as.formula(paste0(metric, "~ Measurement")), 
            paired = TRUE, 
            pool.sd = FALSE,
            var.equal = FALSE,
            p.adjust.method = "holm"
          )
        
        eff_size2 <- model_data %>%
          # filter(!(Animal %in% exclude_animals)) %>%
          group_by(Group) %>%
          cohens_d(
            as.formula(paste0(metric, "~ Measurement")), 
            paired = TRUE, 
            var.equal = FALSE)

        df <- pwc2 %>% 
          left_join(eff_size2) %>% 
          data.frame()
        df %>% 
          kable(escape = F, digits = 3) %>%
          kable_classic_2(full_width = F) %>% 
          kable_styling() %>% 
          row_spec(
            which(df$p.adj <= 0.05), bold = T, 
            background = green_color) %>% 
          print()
        
        cat("\n\n\n<b>===NON-SIGNIFICANT TWO-WAY INTERACTION===</b>\n\n")
        cat("\n\n\n<b>=MAIN EFFECT OF MEASUREMENT=</b>\n\n")
        
        df <- model_data %>%
          # filter(!(Animal %in% exclude_animals)) %>%
          pairwise_t_test(
            as.formula(paste0(metric, "~ Measurement")), 
            paired = TRUE, 
            p.adjust.method = "holm"
          ) %>% 
          data.frame() 
    
        df %>% 
          kable(escape = F) %>%
          kable_classic_2(full_width = F) %>% 
          kable_styling() %>% 
          row_spec(
            which(df$p.adj <= 0.05), bold = T, 
            background = green_color) %>% 
          print()
        
        cat("\n\n\n<b>=MAIN EFFECT OF GROUP=</b>\n\n")
        
        df <- model_data %>%
          pairwise_t_test(
            as.formula(paste0(metric, "~ Group")), 
            p.adjust.method = "holm"
          ) %>% 
          data.frame() 
        
        df %>% 
          kable(escape = F) %>%
          kable_classic_2(full_width = F) %>% 
          kable_styling() %>% 
          row_spec(
            which(df$p.adj <= 0.05), bold = T, 
            background = green_color) %>% 
          print()
        
        cat('\n\n')
        
        cat("##### Plots\n")
        
        pwc <- pwc %>% add_xy_position(x = "Measurement")
        pwc2 <- pwc2 %>% add_xy_position(x = "Group")
        
        box_plot <- ggboxplot(
          model_data, x = "Measurement", y = metric,
          color = "Group", palette = "jco",
          add = "jitter"
        )
        
        box_plot2 <- ggboxplot(
          model_data, x = "Group", y = metric, 
          color = "Measurement", palette = "jco",
          add = "jitter"
        )
        
        p <- box_plot + 
          stat_pvalue_manual(pwc, tip.length = 0.01, hide.ns = TRUE) +
          labs(
            title = paste0(metric, " for ", layer, " Layer, ", freq, "Hz and 1st Click"),
            subtitle = get_test_label(res.aov, detailed = TRUE),
            caption = get_pwc_label(pwc)
          )
        
        plot(p)
        
        cat('\n\n')
        
        p <- box_plot2 + 
          stat_pvalue_manual(pwc2, tip.length = 0.01, hide.ns = TRUE) +
          labs(
            title = paste0(metric, " for ", layer, " Layer, ", freq, "Hz and 1st Click"),
            subtitle = get_test_label(res.aov, detailed = TRUE),
            caption = get_pwc_label(pwc2)
          )
        
        plot(p)

        cat('\n\n')
      
      cat('\n\n')
    }
    
    cat('\n\n')
  }
  
  cat('\n\n')
}

5 Hz Frequency

All Layer

PeakAmp

Assumptions Check

===OUTLIERS===

Group	Measurement	Animal	PeakAmp	PeakLat	RMS	logRMS	is.outlier	is.extreme
KIC	preLaser	KIC04	0.006073	57.82759	0.002878	-5.870151	TRUE	TRUE

===NORMALITY ASSUMPTION===

===HOMOGENEITY OF VARIANCE ASSUMPTION===

Measurement	df1	df2	statistic	p
postLaser	2	24	1.334	0.282
preLaser	2	24	0.410	0.668

Mixed ANOVA

Mixed ANOVA (Type III test)

Effect	DFn	DFd	F	p	p..05	ges
Group	2	24	2.559	0.098		0.141
Measurement	1	24	0.127	0.725		0.001
Group:Measurement	2	24	0.886	0.425		0.017

Post-hoc Tests

===SIGNIFICANT TWO-WAY INTERACTION===

=PAIRWISE COMPARISONS BETWEEN GROUP LEVELS=

Measurement	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
postLaser	PeakAmp	KIC	KIT	10	10	2.691	13.542	0.018	0.054	ns	1.203	large
postLaser	PeakAmp	KIC	KIV	10	7	0.213	14.569	0.835	0.835	ns	0.103	negligible
postLaser	PeakAmp	KIT	KIV	10	7	-2.489	9.437	0.033	0.067	ns	-1.271	large
preLaser	PeakAmp	KIC	KIT	10	10	1.128	12.266	0.281	0.657	ns	0.504	moderate
preLaser	PeakAmp	KIC	KIV	10	7	0.330	12.933	0.747	0.747	ns	0.152	negligible
preLaser	PeakAmp	KIT	KIV	10	7	-1.289	13.265	0.219	0.657	ns	-0.634	moderate

=PAIRWISE COMPARISONS BETWEEN MEASUREMENTS AT EACH GROUP LEVELS=

Group	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
KIC	PeakAmp	postLaser	preLaser	10	10	0.420	9	0.684	0.684	ns	0.133	negligible
KIT	PeakAmp	postLaser	preLaser	10	10	-3.412	9	0.008	0.008	**	-1.079	large
KIV	PeakAmp	postLaser	preLaser	7	7	1.557	6	0.171	0.171	ns	0.588	moderate

===NON-SIGNIFICANT TWO-WAY INTERACTION===

=MAIN EFFECT OF MEASUREMENT=

.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif
PeakAmp	postLaser	preLaser	27	27	0.236018	26	0.815	0.815	ns

=MAIN EFFECT OF GROUP=

.y.	group1	group2	n1	n2	p	p.signif	p.adj	p.adj.signif
PeakAmp	KIC	KIT	20	20	0.00888	**	0.0267
PeakAmp	KIC	KIV	20	14	0.67500	ns	0.6750	ns
PeakAmp	KIT	KIV	20	14	0.04570		0.0915	ns

Plots

PeakLat

Assumptions Check

===OUTLIERS===

Group	Measurement	Animal	PeakAmp	PeakLat	RMS	logRMS	is.outlier	is.extreme
KIT	preLaser	KIT02	0.001913	50.24138	0.001070	-6.862761	TRUE	FALSE
KIT	preLaser	KIT03	0.002493	35.93103	0.001034	-6.885307	TRUE	TRUE
KIT	preLaser	KIT07	0.000649	105.47368	0.000413	-7.810039	TRUE	TRUE

===NORMALITY ASSUMPTION===

===HOMOGENEITY OF VARIANCE ASSUMPTION===

Measurement	df1	df2	statistic	p
postLaser	2	24	0.190	0.828
preLaser	2	24	0.638	0.537

Mixed ANOVA

Mixed ANOVA (Type III test)

Effect	DFn	DFd	F	p	p..05	ges
Group	2	24	1.034	0.371		0.07400
Measurement	1	24	0.255	0.618		0.00074
Group:Measurement	2	24	0.242	0.787		0.00100

Post-hoc Tests

===SIGNIFICANT TWO-WAY INTERACTION===

=PAIRWISE COMPARISONS BETWEEN GROUP LEVELS=

Measurement	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
postLaser	PeakLat	KIC	KIT	10	10	-0.946	17.986	0.357	0.909	ns	-0.423	small
postLaser	PeakLat	KIC	KIV	10	7	-1.082	10.421	0.303	0.909	ns	-0.547	moderate
postLaser	PeakLat	KIT	KIV	10	7	-0.368	10.643	0.720	0.909	ns	-0.185	negligible
preLaser	PeakLat	KIC	KIT	10	10	-1.348	15.778	0.197	0.591	ns	-0.603	moderate
preLaser	PeakLat	KIC	KIV	10	7	-1.310	8.842	0.223	0.591	ns	-0.673	moderate
preLaser	PeakLat	KIT	KIV	10	7	-0.230	11.748	0.822	0.822	ns	-0.115	negligible

=PAIRWISE COMPARISONS BETWEEN MEASUREMENTS AT EACH GROUP LEVELS=

Group	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
KIC	PeakLat	postLaser	preLaser	10	10	0.197	9	0.848	0.848	ns	0.062	negligible
KIT	PeakLat	postLaser	preLaser	10	10	-0.714	9	0.494	0.494	ns	-0.226	small
KIV	PeakLat	postLaser	preLaser	7	7	-0.404	6	0.700	0.700	ns	-0.153	negligible

===NON-SIGNIFICANT TWO-WAY INTERACTION===

=MAIN EFFECT OF MEASUREMENT=

.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif
PeakLat	postLaser	preLaser	27	27	-0.5167219	26	0.61	0.61	ns

=MAIN EFFECT OF GROUP=

.y.	group1	group2	n1	n2	p	p.signif	p.adj	p.adj.signif
PeakLat	KIC	KIT	20	20	0.1340	ns	0.269	ns
PeakLat	KIC	KIV	20	14	0.0667	ns	0.200	ns
PeakLat	KIT	KIV	20	14	0.6240	ns	0.624	ns

Plots

RMS

Assumptions Check

===OUTLIERS===

Group	Measurement	Animal	PeakAmp	PeakLat	RMS	logRMS	is.outlier	is.extreme
KIC	preLaser	KIC04	0.006073	57.82759	0.002878	-5.870151	TRUE	TRUE
KIC	preLaser	KIC10	0.000869	71.14286	0.000525	-7.575743	TRUE	FALSE
KIC	preLaser	KIC15	0.002807	38.13333	0.001258	-6.720152	TRUE	FALSE

===NORMALITY ASSUMPTION===

===HOMOGENEITY OF VARIANCE ASSUMPTION===

Measurement	df1	df2	statistic	p
postLaser	2	24	0.776	0.471
preLaser	2	24	0.144	0.867

Mixed ANOVA

Mixed ANOVA (Type III test)

Effect	DFn	DFd	F	p	p..05	ges
Group	2	24	1.802	0.187		0.107
Measurement	1	24	0.221	0.643		0.002
Group:Measurement	2	24	0.879	0.428		0.015

Post-hoc Tests

===SIGNIFICANT TWO-WAY INTERACTION===

=PAIRWISE COMPARISONS BETWEEN GROUP LEVELS=

Measurement	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
postLaser	RMS	KIC	KIT	10	10	2.251	16.349	0.038	0.115	ns	1.007	large
postLaser	RMS	KIC	KIV	10	7	-0.157	10.881	0.878	0.878	ns	-0.079	negligible
postLaser	RMS	KIT	KIV	10	7	-1.808	8.655	0.105	0.210	ns	-0.931	large
preLaser	RMS	KIC	KIT	10	10	0.948	12.496	0.361	0.723	ns	0.424	small
preLaser	RMS	KIC	KIV	10	7	0.107	13.859	0.916	0.916	ns	0.050	negligible
preLaser	RMS	KIT	KIV	10	7	-1.232	12.211	0.241	0.723	ns	-0.612	moderate

=PAIRWISE COMPARISONS BETWEEN MEASUREMENTS AT EACH GROUP LEVELS=

Group	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
KIC	RMS	postLaser	preLaser	10	10	0.403	9	0.696	0.696	ns	0.127	negligible
KIT	RMS	postLaser	preLaser	10	10	-3.404	9	0.008	0.008	**	-1.077	large
KIV	RMS	postLaser	preLaser	7	7	1.090	6	0.318	0.318	ns	0.412	small

===NON-SIGNIFICANT TWO-WAY INTERACTION===

=MAIN EFFECT OF MEASUREMENT=

.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif
RMS	postLaser	preLaser	27	27	0.3301844	26	0.744	0.744	ns

=MAIN EFFECT OF GROUP=

.y.	group1	group2	n1	n2	p	p.signif	p.adj	p.adj.signif
RMS	KIC	KIT	20	20	0.0353		0.106	ns
RMS	KIC	KIV	20	14	0.9600	ns	0.960	ns
RMS	KIT	KIV	20	14	0.0495		0.106	ns

Plots

logRMS

Assumptions Check

===OUTLIERS===

Group	Measurement	Animal	PeakAmp	PeakLat	RMS	logRMS	is.outlier	is.extreme
KIC	postLaser	KIC10	0.000619	77.69444	0.000371	-7.910550	TRUE	FALSE
KIC	preLaser	KIC03	0.001159	73.80769	0.000731	-7.302110	TRUE	FALSE
KIC	preLaser	KIC04	0.006073	57.82759	0.002878	-5.870151	TRUE	TRUE
KIC	preLaser	KIC10	0.000869	71.14286	0.000525	-7.575743	TRUE	TRUE

===NORMALITY ASSUMPTION===

===HOMOGENEITY OF VARIANCE ASSUMPTION===

Measurement	df1	df2	statistic	p
postLaser	2	24	0.018	0.982
preLaser	2	24	0.107	0.899

Mixed ANOVA

Mixed ANOVA (Type III test)

Effect	DFn	DFd	F	p	p..05	ges
Group	2	24	1.8050000	0.186		1.18e-01
Measurement	1	24	0.0000934	0.992		4.00e-07
Group:Measurement	2	24	1.8850000	0.174		1.70e-02

Post-hoc Tests

===SIGNIFICANT TWO-WAY INTERACTION===

=PAIRWISE COMPARISONS BETWEEN GROUP LEVELS=

Measurement	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
postLaser	logRMS	KIC	KIT	10	10	1.994	17.909	0.062	0.185	ns	0.892	large
postLaser	logRMS	KIC	KIV	10	7	-0.037	13.079	0.971	0.971	ns	-0.018	negligible
postLaser	logRMS	KIT	KIV	10	7	-1.838	12.446	0.090	0.185	ns	-0.911	large
preLaser	logRMS	KIC	KIT	10	10	0.997	17.131	0.333	0.666	ns	0.446	small
preLaser	logRMS	KIC	KIV	10	7	-0.139	14.869	0.892	0.892	ns	-0.066	negligible
preLaser	logRMS	KIT	KIV	10	7	-1.331	14.746	0.203	0.609	ns	-0.642	moderate

=PAIRWISE COMPARISONS BETWEEN MEASUREMENTS AT EACH GROUP LEVELS=

Group	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
KIC	logRMS	postLaser	preLaser	10	10	0.640	9	0.538	0.538	ns	0.202	small
KIT	logRMS	postLaser	preLaser	10	10	-3.601	9	0.006	0.006	**	-1.139	large
KIV	logRMS	postLaser	preLaser	7	7	0.727	6	0.495	0.495	ns	0.275	small

===NON-SIGNIFICANT TWO-WAY INTERACTION===

=MAIN EFFECT OF MEASUREMENT=

.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif
logRMS	postLaser	preLaser	27	27	-0.1174985	26	0.907	0.907	ns

=MAIN EFFECT OF GROUP=

.y.	group1	group2	n1	n2	p	p.signif	p.adj	p.adj.signif
logRMS	KIC	KIT	20	20	0.0286		0.0858	ns
logRMS	KIC	KIV	20	14	0.9080	ns	0.9080	ns
logRMS	KIT	KIV	20	14	0.0355		0.0858	ns

Plots

I_II Layer

PeakAmp

Assumptions Check

===OUTLIERS===

Group	Measurement	Animal	PeakAmp	PeakLat	RMS	logRMS	is.outlier	is.extreme
KIC	postLaser	KIC02	0.005081	56.40816	0.001875	-6.360497	TRUE	TRUE
KIT	postLaser	KIT03	0.002740	55.26531	0.001031	-7.035001	TRUE	FALSE
KIC	preLaser	KIC04	0.008062	67.10345	0.003122	-5.888868	TRUE	TRUE

===NORMALITY ASSUMPTION===

===HOMOGENEITY OF VARIANCE ASSUMPTION===

Measurement	df1	df2	statistic	p
postLaser	2	23	3.000	0.070
preLaser	2	23	1.417	0.263

Mixed ANOVA

Mixed ANOVA (Type III test)

Effect	DFn	DFd	F	p	p..05	ges
Group	2	23	2.291	0.124		0.138000
Measurement	1	23	0.043	0.837		0.000377
Group:Measurement	2	23	0.122	0.886		0.002000

Post-hoc Tests

===SIGNIFICANT TWO-WAY INTERACTION===

=PAIRWISE COMPARISONS BETWEEN GROUP LEVELS=

Measurement	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
postLaser	PeakAmp	KIC	KIT	10	9	1.528	12.959	0.150	0.390	ns	0.690	moderate
postLaser	PeakAmp	KIC	KIV	10	7	-1.012	7.934	0.342	0.390	ns	-0.526	moderate
postLaser	PeakAmp	KIT	KIV	9	7	-1.736	6.456	0.130	0.390	ns	-0.923	large
preLaser	PeakAmp	KIC	KIT	10	9	1.424	10.169	0.184	0.483	ns	0.639	moderate
preLaser	PeakAmp	KIC	KIV	10	7	-0.617	10.271	0.551	0.551	ns	-0.312	small
preLaser	PeakAmp	KIT	KIV	9	7	-1.591	6.288	0.161	0.483	ns	-0.847	large

=PAIRWISE COMPARISONS BETWEEN MEASUREMENTS AT EACH GROUP LEVELS=

Group	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
KIC	PeakAmp	postLaser	preLaser	10	10	-0.347	9	0.737	0.737	ns	-0.110	negligible
KIT	PeakAmp	postLaser	preLaser	9	9	1.032	8	0.332	0.332	ns	0.344	small
KIV	PeakAmp	postLaser	preLaser	7	7	-0.100	6	0.924	0.924	ns	-0.038	negligible

===NON-SIGNIFICANT TWO-WAY INTERACTION===

=MAIN EFFECT OF MEASUREMENT=

.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif
PeakAmp	postLaser	preLaser	26	26	-0.2476079	25	0.806	0.806	ns

=MAIN EFFECT OF GROUP=

.y.	group1	group2	n1	n2	p	p.signif	p.adj	p.adj.signif
PeakAmp	KIC	KIT	20	18	0.15500	ns	0.2770	ns
PeakAmp	KIC	KIV	20	14	0.13800	ns	0.2770	ns
PeakAmp	KIT	KIV	18	14	0.00751	**	0.0225

Plots

PeakLat

Assumptions Check

===OUTLIERS===

No outliers were detected.

===NORMALITY ASSUMPTION===

===HOMOGENEITY OF VARIANCE ASSUMPTION===

Measurement	df1	df2	statistic	p
postLaser	2	23	0.091	0.913
preLaser	2	23	0.317	0.731

Mixed ANOVA

Mixed ANOVA (Type III test)

Effect	DFn	DFd	F	p	p..05	ges
Group	2	23	1.989	0.160		0.110
Measurement	1	23	1.008	0.326		0.012
Group:Measurement	2	23	0.164	0.849		0.004

Post-hoc Tests

===SIGNIFICANT TWO-WAY INTERACTION===

=PAIRWISE COMPARISONS BETWEEN GROUP LEVELS=

Measurement	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
postLaser	PeakLat	KIC	KIT	10	9	-0.345	16.450	0.734	0.734	ns	-0.159	negligible
postLaser	PeakLat	KIC	KIV	10	7	1.433	13.856	0.174	0.348	ns	0.700	moderate
postLaser	PeakLat	KIT	KIV	9	7	1.681	13.858	0.115	0.345	ns	0.838	large
preLaser	PeakLat	KIC	KIT	10	9	-0.827	16.995	0.420	0.664	ns	-0.379	small
preLaser	PeakLat	KIC	KIV	10	7	1.003	14.966	0.332	0.664	ns	0.476	small
preLaser	PeakLat	KIT	KIV	9	7	1.957	13.941	0.071	0.212	ns	0.966	large

=PAIRWISE COMPARISONS BETWEEN MEASUREMENTS AT EACH GROUP LEVELS=

Group	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
KIC	PeakLat	postLaser	preLaser	10	10	-0.102	9	0.921	0.921	ns	-0.032	negligible
KIT	PeakLat	postLaser	preLaser	9	9	-1.284	8	0.235	0.235	ns	-0.428	small
KIV	PeakLat	postLaser	preLaser	7	7	-1.545	6	0.173	0.173	ns	-0.584	moderate

===NON-SIGNIFICANT TWO-WAY INTERACTION===

=MAIN EFFECT OF MEASUREMENT=

.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif
PeakLat	postLaser	preLaser	26	26	-0.975698	25	0.339	0.339	ns

=MAIN EFFECT OF GROUP=

.y.	group1	group2	n1	n2	p	p.signif	p.adj	p.adj.signif
PeakLat	KIC	KIT	20	18	0.3840	ns	0.3840	ns
PeakLat	KIC	KIV	20	14	0.1040	ns	0.2070	ns
PeakLat	KIT	KIV	18	14	0.0191		0.0574	ns

Plots

RMS

Assumptions Check

===OUTLIERS===

Group	Measurement	Animal	PeakAmp	PeakLat	RMS	logRMS	is.outlier	is.extreme
KIC	preLaser	KIC04	0.008062	67.10345	0.003122	-5.888868	TRUE	TRUE

===NORMALITY ASSUMPTION===

===HOMOGENEITY OF VARIANCE ASSUMPTION===

Measurement	df1	df2	statistic	p
postLaser	2	23	4.967	0.016
preLaser	2	23	2.349	0.118

Mixed ANOVA

Mixed ANOVA (Type III test)

Effect	DFn	DFd	F	p	p..05	ges
Group	2	23	3.016	0.069		1.83e-01
Measurement	1	23	0.003	0.960		1.65e-05
Group:Measurement	2	23	0.064	0.938		8.27e-04

Post-hoc Tests

===SIGNIFICANT TWO-WAY INTERACTION===

=PAIRWISE COMPARISONS BETWEEN GROUP LEVELS=

Measurement	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
postLaser	RMS	KIC	KIT	10	9	2.336	13.645	0.035	0.106	ns	1.056	large
postLaser	RMS	KIC	KIV	10	7	-0.921	7.509	0.385	0.385	ns	-0.481	small
postLaser	RMS	KIT	KIV	9	7	-1.879	6.430	0.106	0.212	ns	-0.999	large
preLaser	RMS	KIC	KIT	10	9	1.950	10.665	0.078	0.234	ns	0.876	large
preLaser	RMS	KIC	KIV	10	7	-0.691	9.174	0.507	0.507	ns	-0.354	small
preLaser	RMS	KIT	KIV	9	7	-1.805	6.300	0.119	0.238	ns	-0.962	large

=PAIRWISE COMPARISONS BETWEEN MEASUREMENTS AT EACH GROUP LEVELS=

Group	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
KIC	RMS	postLaser	preLaser	10	10	-0.238	9	0.818	0.818	ns	-0.075	negligible
KIT	RMS	postLaser	preLaser	9	9	0.690	8	0.510	0.510	ns	0.230	small
KIV	RMS	postLaser	preLaser	7	7	-0.006	6	0.996	0.996	ns	-0.002	negligible

===NON-SIGNIFICANT TWO-WAY INTERACTION===

=MAIN EFFECT OF MEASUREMENT=

.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif
RMS	postLaser	preLaser	26	26	-0.073719	25	0.942	0.942	ns

=MAIN EFFECT OF GROUP=

.y.	group1	group2	n1	n2	p	p.signif	p.adj	p.adj.signif
RMS	KIC	KIT	20	18	0.0608	ns	0.12200	ns
RMS	KIC	KIV	20	14	0.1230	ns	0.12300	ns
RMS	KIT	KIV	18	14	0.0019	**	0.00569	**

Plots

logRMS

Assumptions Check

===OUTLIERS===

Group	Measurement	Animal	PeakAmp	PeakLat	RMS	logRMS	is.outlier	is.extreme
KIC	preLaser	KIC04	0.008062	67.10345	0.003122	-5.888868	TRUE	FALSE

===NORMALITY ASSUMPTION===

===HOMOGENEITY OF VARIANCE ASSUMPTION===

Measurement	df1	df2	statistic	p
postLaser	2	23	3.597	0.044
preLaser	2	23	1.549	0.234

Mixed ANOVA

Mixed ANOVA (Type III test)

Effect	DFn	DFd	F	p	p..05	ges
Group	2	23	2.355	0.117		0.158000
Measurement	1	23	0.170	0.684		0.000594
Group:Measurement	2	23	0.258	0.775		0.002000

Post-hoc Tests

===SIGNIFICANT TWO-WAY INTERACTION===

=PAIRWISE COMPARISONS BETWEEN GROUP LEVELS=

Measurement	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
postLaser	logRMS	KIC	KIT	10	9	1.933	16.155	0.071	0.213	ns	0.880	large
postLaser	logRMS	KIC	KIV	10	7	-0.471	8.632	0.649	0.649	ns	-0.243	small
postLaser	logRMS	KIT	KIV	9	7	-1.554	7.457	0.162	0.324	ns	-0.818	large
preLaser	logRMS	KIC	KIT	10	9	2.179	15.641	0.045	0.135	ns	0.990	large
preLaser	logRMS	KIC	KIV	10	7	-0.260	10.820	0.800	0.800	ns	-0.131	negligible
preLaser	logRMS	KIT	KIV	9	7	-1.848	8.394	0.100	0.200	ns	-0.965	large

=PAIRWISE COMPARISONS BETWEEN MEASUREMENTS AT EACH GROUP LEVELS=

Group	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
KIC	logRMS	postLaser	preLaser	10	10	-0.258	9	0.802	0.802	ns	-0.082	negligible
KIT	logRMS	postLaser	preLaser	9	9	0.897	8	0.396	0.396	ns	0.299	small
KIV	logRMS	postLaser	preLaser	7	7	0.555	6	0.599	0.599	ns	0.210	small

===NON-SIGNIFICANT TWO-WAY INTERACTION===

=MAIN EFFECT OF MEASUREMENT=

.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif
logRMS	postLaser	preLaser	26	26	0.3584085	25	0.723	0.723	ns

=MAIN EFFECT OF GROUP=

.y.	group1	group2	n1	n2	p	p.signif	p.adj	p.adj.signif
logRMS	KIC	KIT	20	18	0.02240		0.0448
logRMS	KIC	KIV	20	14	0.50600	ns	0.5060	ns
logRMS	KIT	KIV	18	14	0.00718	**	0.0215

Plots

IV Layer

PeakAmp

Assumptions Check

===OUTLIERS===

Group	Measurement	Animal	PeakAmp	PeakLat	RMS	logRMS	is.outlier	is.extreme
KIC	preLaser	KIC04	0.011819	52.2069	0.005815	-5.197666	TRUE	TRUE
KIC	preLaser	KIC15	0.005346	37.5000	0.002049	-6.461238	TRUE	FALSE

===NORMALITY ASSUMPTION===

===HOMOGENEITY OF VARIANCE ASSUMPTION===

Measurement	df1	df2	statistic	p
postLaser	2	24	0.332	0.721
preLaser	2	24	0.064	0.938

Mixed ANOVA

Mixed ANOVA (Type III test)

Effect	DFn	DFd	F	p	p..05	ges
Group	2	24	1.019	0.376		0.067000
Measurement	1	24	0.141	0.710		0.000909
Group:Measurement	2	24	0.843	0.443		0.011000

Post-hoc Tests

===SIGNIFICANT TWO-WAY INTERACTION===

=PAIRWISE COMPARISONS BETWEEN GROUP LEVELS=

Measurement	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
postLaser	PeakAmp	KIC	KIT	10	10	2.012	16.791	0.060	0.182	ns	0.900	large
postLaser	PeakAmp	KIC	KIV	10	7	-0.101	12.037	0.921	0.921	ns	-0.050	negligible
postLaser	PeakAmp	KIT	KIV	10	7	-1.748	9.789	0.112	0.224	ns	-0.889	large
preLaser	PeakAmp	KIC	KIT	10	10	0.514	14.673	0.615	1.000	ns	0.230	small
preLaser	PeakAmp	KIC	KIV	10	7	-0.174	14.951	0.864	1.000	ns	-0.083	negligible
preLaser	PeakAmp	KIT	KIV	10	7	-0.840	12.382	0.417	1.000	ns	-0.417	small

=PAIRWISE COMPARISONS BETWEEN MEASUREMENTS AT EACH GROUP LEVELS=

Group	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
KIC	PeakAmp	postLaser	preLaser	10	10	0.263	9	0.798	0.798	ns	0.083	negligible
KIT	PeakAmp	postLaser	preLaser	10	10	-3.191	9	0.011	0.011		-1.009	large
KIV	PeakAmp	postLaser	preLaser	7	7	0.668	6	0.529	0.529	ns	0.253	small

===NON-SIGNIFICANT TWO-WAY INTERACTION===

=MAIN EFFECT OF MEASUREMENT=

.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif
PeakAmp	postLaser	preLaser	27	27	-0.4618568	26	0.648	0.648	ns

=MAIN EFFECT OF GROUP=

.y.	group1	group2	n1	n2	p	p.signif	p.adj	p.adj.signif
PeakAmp	KIC	KIT	20	20	0.119	ns	0.312	ns
PeakAmp	KIC	KIV	20	14	0.828	ns	0.828	ns
PeakAmp	KIT	KIV	20	14	0.104	ns	0.312	ns

Plots

PeakLat

Assumptions Check

===OUTLIERS===

No outliers were detected.

===NORMALITY ASSUMPTION===

===HOMOGENEITY OF VARIANCE ASSUMPTION===

Measurement	df1	df2	statistic	p
postLaser	2	24	0.231	0.796
preLaser	2	24	0.335	0.719

Mixed ANOVA

Mixed ANOVA (Type III test)

Effect	DFn	DFd	F	p	p..05	ges
Group	2	24	1.021	0.375		7.3e-02
Measurement	1	24	0.033	0.858		9.5e-05
Group:Measurement	2	24	0.699	0.507		4.0e-03

Post-hoc Tests

===SIGNIFICANT TWO-WAY INTERACTION===

=PAIRWISE COMPARISONS BETWEEN GROUP LEVELS=

Measurement	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
postLaser	PeakLat	KIC	KIT	10	10	-0.622	17.057	0.542	0.816	ns	-0.278	small
postLaser	PeakLat	KIC	KIV	10	7	-1.413	9.743	0.189	0.567	ns	-0.719	moderate
postLaser	PeakLat	KIT	KIV	10	7	-0.859	11.662	0.408	0.816	ns	-0.429	small
preLaser	PeakLat	KIC	KIT	10	10	-0.013	16.916	0.990	0.990	ns	-0.006	negligible
preLaser	PeakLat	KIC	KIV	10	7	-1.054	8.903	0.319	0.957	ns	-0.541	moderate
preLaser	PeakLat	KIT	KIV	10	7	-0.982	10.680	0.348	0.957	ns	-0.495	small

=PAIRWISE COMPARISONS BETWEEN MEASUREMENTS AT EACH GROUP LEVELS=

Group	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
KIC	PeakLat	postLaser	preLaser	10	10	-0.959	9	0.363	0.363	ns	-0.303	small
KIT	PeakLat	postLaser	preLaser	10	10	0.760	9	0.467	0.467	ns	0.240	small
KIV	PeakLat	postLaser	preLaser	7	7	0.328	6	0.754	0.754	ns	0.124	negligible

===NON-SIGNIFICANT TWO-WAY INTERACTION===

=MAIN EFFECT OF MEASUREMENT=

.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif
PeakLat	postLaser	preLaser	27	27	0.1185458	26	0.907	0.907	ns

=MAIN EFFECT OF GROUP=

.y.	group1	group2	n1	n2	p	p.signif	p.adj	p.adj.signif
PeakLat	KIC	KIT	20	20	0.6590	ns	0.659	ns
PeakLat	KIC	KIV	20	14	0.0574	ns	0.172	ns
PeakLat	KIT	KIV	20	14	0.1300	ns	0.259	ns

Plots

RMS

Assumptions Check

===OUTLIERS===

Group	Measurement	Animal	PeakAmp	PeakLat	RMS	logRMS	is.outlier	is.extreme
KIC	preLaser	KIC04	0.011819	52.2069	0.005815	-5.197666	TRUE	TRUE
KIC	preLaser	KIC15	0.005346	37.5000	0.002049	-6.461238	TRUE	FALSE

===NORMALITY ASSUMPTION===

===HOMOGENEITY OF VARIANCE ASSUMPTION===

Measurement	df1	df2	statistic	p
postLaser	2	24	0.881	0.427
preLaser	2	24	0.075	0.928

Mixed ANOVA

Mixed ANOVA (Type III test)

Effect	DFn	DFd	F	p	p..05	ges
Group	2	24	0.981	0.390		0.062000
Measurement	1	24	0.025	0.876		0.000199
Group:Measurement	2	24	0.549	0.585		0.009000

Post-hoc Tests

===SIGNIFICANT TWO-WAY INTERACTION===

=PAIRWISE COMPARISONS BETWEEN GROUP LEVELS=

Measurement	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
postLaser	RMS	KIC	KIT	10	10	1.857	16.974	0.081	0.242	ns	0.831	large
postLaser	RMS	KIC	KIV	10	7	-0.329	11.025	0.748	0.748	ns	-0.165	negligible
postLaser	RMS	KIT	KIV	10	7	-1.739	9.191	0.115	0.242	ns	-0.890	large
preLaser	RMS	KIC	KIT	10	10	0.557	12.792	0.587	1.000	ns	0.249	small
preLaser	RMS	KIC	KIV	10	7	-0.059	14.678	0.954	1.000	ns	-0.028	negligible
preLaser	RMS	KIT	KIV	10	7	-0.817	11.150	0.431	1.000	ns	-0.410	small

=PAIRWISE COMPARISONS BETWEEN MEASUREMENTS AT EACH GROUP LEVELS=

Group	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
KIC	RMS	postLaser	preLaser	10	10	0.082	9	0.936	0.936	ns	0.026	negligible
KIT	RMS	postLaser	preLaser	10	10	-3.348	9	0.009	0.009	**	-1.059	large
KIV	RMS	postLaser	preLaser	7	7	0.831	6	0.438	0.438	ns	0.314	small

===NON-SIGNIFICANT TWO-WAY INTERACTION===

=MAIN EFFECT OF MEASUREMENT=

.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif
RMS	postLaser	preLaser	27	27	-0.2837388	26	0.779	0.779	ns

=MAIN EFFECT OF GROUP=

.y.	group1	group2	n1	n2	p	p.signif	p.adj	p.adj.signif
RMS	KIC	KIT	20	20	0.144	ns	0.332	ns
RMS	KIC	KIV	20	14	0.781	ns	0.781	ns
RMS	KIT	KIV	20	14	0.111	ns	0.332	ns

Plots

logRMS

Assumptions Check

===OUTLIERS===

Group	Measurement	Animal	PeakAmp	PeakLat	RMS	logRMS	is.outlier	is.extreme
KIC	postLaser	KIC10	0.000693	67.33333	0.000306	-8.137241	TRUE	FALSE
KIC	preLaser	KIC04	0.011819	52.20690	0.005815	-5.197666	TRUE	TRUE
KIC	preLaser	KIC10	0.001174	73.76667	0.000523	-7.695218	TRUE	FALSE

===NORMALITY ASSUMPTION===

===HOMOGENEITY OF VARIANCE ASSUMPTION===

Measurement	df1	df2	statistic	p
postLaser	2	24	0.012	0.988
preLaser	2	24	0.557	0.580

Mixed ANOVA

Mixed ANOVA (Type III test)

Effect	DFn	DFd	F	p	p..05	ges
Group	2	24	1.170	0.327		0.081000
Measurement	1	24	0.232	0.635		0.000964
Group:Measurement	2	24	2.998	0.069		0.024000

Post-hoc Tests

===SIGNIFICANT TWO-WAY INTERACTION===

=PAIRWISE COMPARISONS BETWEEN GROUP LEVELS=

Measurement	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
postLaser	logRMS	KIC	KIT	10	10	1.780	17.971	0.092	0.250	ns	0.796	moderate
postLaser	logRMS	KIC	KIV	10	7	-0.152	13.933	0.882	0.882	ns	-0.074	negligible
postLaser	logRMS	KIT	KIV	10	7	-1.867	13.626	0.084	0.250	ns	-0.915	large
preLaser	logRMS	KIC	KIT	10	10	0.458	17.697	0.653	1.000	ns	0.205	small
preLaser	logRMS	KIC	KIV	10	7	-0.298	14.489	0.770	1.000	ns	-0.140	negligible
preLaser	logRMS	KIT	KIV	10	7	-0.878	14.931	0.394	1.000	ns	-0.416	small

=PAIRWISE COMPARISONS BETWEEN MEASUREMENTS AT EACH GROUP LEVELS=

Group	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
KIC	logRMS	postLaser	preLaser	10	10	0.606	9	0.560	0.560	ns	0.192	negligible
KIT	logRMS	postLaser	preLaser	10	10	-5.065	9	0.001	0.001	***	-1.602	large
KIV	logRMS	postLaser	preLaser	7	7	0.574	6	0.587	0.587	ns	0.217	small

===NON-SIGNIFICANT TWO-WAY INTERACTION===

=MAIN EFFECT OF MEASUREMENT=

.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif
logRMS	postLaser	preLaser	27	27	-0.606959	26	0.549	0.549	ns

=MAIN EFFECT OF GROUP=

.y.	group1	group2	n1	n2	p	p.signif	p.adj	p.adj.signif
logRMS	KIC	KIT	20	20	0.0937	ns	0.212	ns
logRMS	KIC	KIV	20	14	0.7690	ns	0.769	ns
logRMS	KIT	KIV	20	14	0.0708	ns	0.212	ns

Plots

V Layer

PeakAmp

Assumptions Check

===OUTLIERS===

Group	Measurement	Animal	PeakAmp	PeakLat	RMS	logRMS	is.outlier	is.extreme
KIC	postLaser	KIC10	0.000695	71.20408	0.000326	-8.085219	TRUE	FALSE
KIC	postLaser	KIC11	0.004177	44.02128	0.001739	-6.605032	TRUE	FALSE

===NORMALITY ASSUMPTION===

===HOMOGENEITY OF VARIANCE ASSUMPTION===

Measurement	df1	df2	statistic	p
postLaser	2	24	1.187	0.322
preLaser	2	24	0.008	0.992

Mixed ANOVA

Mixed ANOVA (Type III test)

Effect	DFn	DFd	F	p	p..05	ges
Group	2	24	2.213	0.131		0.142
Measurement	1	24	2.280	0.144		0.010
Group:Measurement	2	24	3.225	0.057		0.027

Post-hoc Tests

===SIGNIFICANT TWO-WAY INTERACTION===

=PAIRWISE COMPARISONS BETWEEN GROUP LEVELS=

Measurement	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
postLaser	PeakAmp	KIC	KIT	10	10	2.317	14.996	0.035	0.105	ns	1.036	large
postLaser	PeakAmp	KIC	KIV	10	7	-0.289	12.276	0.778	0.778	ns	-0.143	negligible
postLaser	PeakAmp	KIT	KIV	10	7	-2.212	8.691	0.055	0.111	ns	-1.139	large
preLaser	PeakAmp	KIC	KIT	10	10	1.031	17.927	0.316	0.783	ns	0.461	small
preLaser	PeakAmp	KIC	KIV	10	7	-0.273	12.599	0.789	0.789	ns	-0.135	negligible
preLaser	PeakAmp	KIT	KIV	10	7	-1.179	12.025	0.261	0.783	ns	-0.587	moderate

=PAIRWISE COMPARISONS BETWEEN MEASUREMENTS AT EACH GROUP LEVELS=

Group	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
KIC	PeakAmp	postLaser	preLaser	10	10	1.948	9	0.083	0.083	ns	0.616	moderate
KIT	PeakAmp	postLaser	preLaser	10	10	-1.690	9	0.125	0.125	ns	-0.535	moderate
KIV	PeakAmp	postLaser	preLaser	7	7	1.317	6	0.236	0.236	ns	0.498	small

===NON-SIGNIFICANT TWO-WAY INTERACTION===

=MAIN EFFECT OF MEASUREMENT=

.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif
PeakAmp	postLaser	preLaser	27	27	1.211925	26	0.236	0.236	ns

=MAIN EFFECT OF GROUP=

.y.	group1	group2	n1	n2	p	p.signif	p.adj	p.adj.signif
PeakAmp	KIC	KIT	20	20	0.0249		0.0498
PeakAmp	KIC	KIV	20	14	0.6570	ns	0.6570	ns
PeakAmp	KIT	KIV	20	14	0.0140		0.0421

Plots

PeakLat

Assumptions Check

===OUTLIERS===

Group	Measurement	Animal	PeakAmp	PeakLat	RMS	logRMS	is.outlier	is.extreme
KIV	postLaser	KIV12	0.001747	45.46939	0.000526	-7.623111	TRUE	FALSE
KIV	postLaser	KIV17	0.001239	95.68085	0.000681	-7.377188	TRUE	FALSE
KIV	preLaser	KIV12	0.001894	32.17241	0.000583	-7.503453	TRUE	FALSE

===NORMALITY ASSUMPTION===

===HOMOGENEITY OF VARIANCE ASSUMPTION===

Measurement	df1	df2	statistic	p
postLaser	2	24	0.042	0.959
preLaser	2	24	0.464	0.634

Mixed ANOVA

Mixed ANOVA (Type III test)

Effect	DFn	DFd	F	p	p..05	ges
Group	2	24	0.988	0.387		6.80e-02
Measurement	1	24	0.017	0.899		8.25e-05
Group:Measurement	2	24	0.479	0.625		5.00e-03

Post-hoc Tests

===SIGNIFICANT TWO-WAY INTERACTION===

=PAIRWISE COMPARISONS BETWEEN GROUP LEVELS=

Measurement	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
postLaser	PeakLat	KIC	KIT	10	10	-0.291	17.755	0.774	0.774	ns	-0.130	negligible
postLaser	PeakLat	KIC	KIV	10	7	-1.209	11.535	0.251	0.753	ns	-0.605	moderate
postLaser	PeakLat	KIT	KIV	10	7	-0.917	12.591	0.376	0.753	ns	-0.454	small
preLaser	PeakLat	KIC	KIT	10	10	0.333	16.946	0.743	0.813	ns	0.149	negligible
preLaser	PeakLat	KIC	KIV	10	7	-0.997	8.773	0.345	0.813	ns	-0.513	moderate
preLaser	PeakLat	KIT	KIV	10	7	-1.162	10.459	0.271	0.813	ns	-0.587	moderate

=PAIRWISE COMPARISONS BETWEEN MEASUREMENTS AT EACH GROUP LEVELS=

Group	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
KIC	PeakLat	postLaser	preLaser	10	10	-0.370	9	0.720	0.720	ns	-0.117	negligible
KIT	PeakLat	postLaser	preLaser	10	10	1.125	9	0.290	0.290	ns	0.356	small
KIV	PeakLat	postLaser	preLaser	7	7	-0.401	6	0.703	0.703	ns	-0.151	negligible

===NON-SIGNIFICANT TWO-WAY INTERACTION===

=MAIN EFFECT OF MEASUREMENT=

.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif
PeakLat	postLaser	preLaser	27	27	-0.043104	26	0.966	0.966	ns

=MAIN EFFECT OF GROUP=

.y.	group1	group2	n1	n2	p	p.signif	p.adj	p.adj.signif
PeakLat	KIC	KIT	20	20	0.9710	ns	0.971	ns
PeakLat	KIC	KIV	20	14	0.0966	ns	0.271	ns
PeakLat	KIT	KIV	20	14	0.0904	ns	0.271	ns

Plots

RMS

Assumptions Check

===OUTLIERS===

Group	Measurement	Animal	PeakAmp	PeakLat	RMS	logRMS	is.outlier	is.extreme
KIC	postLaser	KIC11	0.004177	44.02128	0.001739	-6.605032	TRUE	FALSE
KIV	preLaser	KIV09	0.003272	77.37931	0.001454	-6.618861	TRUE	FALSE

===NORMALITY ASSUMPTION===

===HOMOGENEITY OF VARIANCE ASSUMPTION===

Measurement	df1	df2	statistic	p
postLaser	2	24	0.846	0.441
preLaser	2	24	0.029	0.971

Mixed ANOVA

Mixed ANOVA (Type III test)

Effect	DFn	DFd	F	p	p..05	ges
Group	2	24	1.817	0.184		0.114
Measurement	1	24	4.381	0.047		0.027
Group:Measurement	2	24	3.439	0.049		0.042

Post-hoc Tests

===SIGNIFICANT TWO-WAY INTERACTION===

=PAIRWISE COMPARISONS BETWEEN GROUP LEVELS=

Measurement	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
postLaser	RMS	KIC	KIT	10	10	1.867	15.127	0.081	0.163	ns	0.835	large
postLaser	RMS	KIC	KIV	10	7	-0.603	12.504	0.557	0.557	ns	-0.299	small
postLaser	RMS	KIT	KIV	10	7	-2.239	8.908	0.052	0.157	ns	-1.150	large
preLaser	RMS	KIC	KIT	10	10	0.435	17.974	0.668	1.000	ns	0.195	negligible
preLaser	RMS	KIC	KIV	10	7	-0.543	10.339	0.599	1.000	ns	-0.275	small
preLaser	RMS	KIT	KIV	10	7	-0.860	10.639	0.409	1.000	ns	-0.434	small

=PAIRWISE COMPARISONS BETWEEN MEASUREMENTS AT EACH GROUP LEVELS=

Group	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
KIC	RMS	postLaser	preLaser	10	10	2.281	9	0.048	0.048		0.721	moderate
KIT	RMS	postLaser	preLaser	10	10	-1.354	9	0.209	0.209	ns	-0.428	small
KIV	RMS	postLaser	preLaser	7	7	1.584	6	0.164	0.164	ns	0.599	moderate

===NON-SIGNIFICANT TWO-WAY INTERACTION===

=MAIN EFFECT OF MEASUREMENT=

.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif
RMS	postLaser	preLaser	27	27	1.710984	26	0.099	0.099	ns

=MAIN EFFECT OF GROUP=

.y.	group1	group2	n1	n2	p	p.signif	p.adj	p.adj.signif
RMS	KIC	KIT	20	20	0.1080	ns	0.2150	ns
RMS	KIC	KIV	20	14	0.3650	ns	0.3650	ns
RMS	KIT	KIV	20	14	0.0201		0.0602	ns

Plots

logRMS

Assumptions Check

===OUTLIERS===

Group	Measurement	Animal	PeakAmp	PeakLat	RMS	logRMS	is.outlier	is.extreme
KIT	preLaser	KIT07	0.000546	94.34615	0.000237	-8.407507	TRUE	FALSE
KIV	preLaser	KIV09	0.003272	77.37931	0.001454	-6.618861	TRUE	FALSE

===NORMALITY ASSUMPTION===

===HOMOGENEITY OF VARIANCE ASSUMPTION===

Measurement	df1	df2	statistic	p
postLaser	2	24	0.004	0.996
preLaser	2	24	0.189	0.829

Mixed ANOVA

Mixed ANOVA (Type III test)

Effect	DFn	DFd	F	p	p..05	ges
Group	2	24	1.496	0.244		0.101
Measurement	1	24	1.143	0.296		0.005
Group:Measurement	2	24	3.218	0.058		0.026

Post-hoc Tests

===SIGNIFICANT TWO-WAY INTERACTION===

=PAIRWISE COMPARISONS BETWEEN GROUP LEVELS=

Measurement	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
postLaser	logRMS	KIC	KIT	10	10	1.686	17.996	0.109	0.218	ns	0.754	moderate
postLaser	logRMS	KIC	KIV	10	7	-0.478	13.896	0.640	0.640	ns	-0.234	small
postLaser	logRMS	KIT	KIV	10	7	-2.097	14.000	0.055	0.164	ns	-1.023	large
preLaser	logRMS	KIC	KIT	10	10	0.490	16.928	0.630	1.000	ns	0.219	small
preLaser	logRMS	KIC	KIV	10	7	-0.612	13.128	0.551	1.000	ns	-0.301	small
preLaser	logRMS	KIT	KIV	10	7	-0.997	14.797	0.335	1.000	ns	-0.480	small

=PAIRWISE COMPARISONS BETWEEN MEASUREMENTS AT EACH GROUP LEVELS=

Group	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
KIC	logRMS	postLaser	preLaser	10	10	1.896	9	0.090	0.090	ns	0.600	moderate
KIT	logRMS	postLaser	preLaser	10	10	-1.584	9	0.148	0.148	ns	-0.501	moderate
KIV	logRMS	postLaser	preLaser	7	7	1.196	6	0.277	0.277	ns	0.452	small

===NON-SIGNIFICANT TWO-WAY INTERACTION===

=MAIN EFFECT OF MEASUREMENT=

.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif
logRMS	postLaser	preLaser	27	27	0.8209969	26	0.419	0.419	ns

=MAIN EFFECT OF GROUP=

.y.	group1	group2	n1	n2	p	p.signif	p.adj	p.adj.signif
logRMS	KIC	KIT	20	20	0.0992	ns	0.198	ns
logRMS	KIC	KIV	20	14	0.4790	ns	0.479	ns
logRMS	KIT	KIV	20	14	0.0297		0.089	ns

Plots

VI Layer

PeakAmp

Assumptions Check

===OUTLIERS===

Group	Measurement	Animal	PeakAmp	PeakLat	RMS	logRMS	is.outlier	is.extreme
KIC	postLaser	KIC03	0.000994	85.84444	0.000461	-7.836092	TRUE	FALSE
KIC	postLaser	KIC10	0.000900	93.36735	0.000383	-7.915954	TRUE	FALSE
KIC	postLaser	KIC11	0.002631	80.82979	0.001244	-6.905797	TRUE	FALSE
KIC	postLaser	KIC15	0.003108	24.08163	0.000997	-6.979746	TRUE	TRUE
KIV	preLaser	KIV03	0.002418	77.20000	0.000971	-7.136828	TRUE	FALSE

===NORMALITY ASSUMPTION===

===HOMOGENEITY OF VARIANCE ASSUMPTION===

Measurement	df1	df2	statistic	p
postLaser	2	24	0.362	0.700
preLaser	2	24	0.654	0.529

Mixed ANOVA

Mixed ANOVA (Type III test)

Effect	DFn	DFd	F	p	p..05	ges
Group	2	24	0.982	0.389		0.072000
Measurement	1	24	0.384	0.541		0.000941
Group:Measurement	2	24	4.766	0.018		0.023000

Post-hoc Tests

===SIGNIFICANT TWO-WAY INTERACTION===

=PAIRWISE COMPARISONS BETWEEN GROUP LEVELS=

Measurement	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
postLaser	PeakAmp	KIC	KIT	10	10	1.471	15.053	0.162	0.324	ns	0.658	moderate
postLaser	PeakAmp	KIC	KIV	10	7	0.008	14.815	0.994	0.994	ns	0.004	negligible
postLaser	PeakAmp	KIT	KIV	10	7	-1.801	13.268	0.094	0.283	ns	-0.886	large
preLaser	PeakAmp	KIC	KIT	10	10	0.234	17.921	0.817	0.817	ns	0.105	negligible
preLaser	PeakAmp	KIC	KIV	10	7	-1.020	14.384	0.324	0.726	ns	-0.495	small
preLaser	PeakAmp	KIT	KIV	10	7	-1.220	14.714	0.242	0.726	ns	-0.589	moderate

=PAIRWISE COMPARISONS BETWEEN MEASUREMENTS AT EACH GROUP LEVELS=

Group	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
KIC	PeakAmp	postLaser	preLaser	10	10	2.072	9	0.068	0.068	ns	0.655	moderate
KIT	PeakAmp	postLaser	preLaser	10	10	-2.132	9	0.062	0.062	ns	-0.674	moderate
KIV	PeakAmp	postLaser	preLaser	7	7	-0.002	6	0.999	0.999	ns	-0.001	negligible

===NON-SIGNIFICANT TWO-WAY INTERACTION===

=MAIN EFFECT OF MEASUREMENT=

.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif
PeakAmp	postLaser	preLaser	27	27	0.614848	26	0.544	0.544	ns

=MAIN EFFECT OF GROUP=

.y.	group1	group2	n1	n2	p	p.signif	p.adj	p.adj.signif
PeakAmp	KIC	KIT	20	20	0.1710	ns	0.343	ns
PeakAmp	KIC	KIV	20	14	0.5500	ns	0.550	ns
PeakAmp	KIT	KIV	20	14	0.0686	ns	0.206	ns

Plots

PeakLat

Assumptions Check

===OUTLIERS===

Group	Measurement	Animal	PeakAmp	PeakLat	RMS	logRMS	is.outlier	is.extreme
KIC	postLaser	KIC15	0.003108	24.08163	0.000997	-6.979746	TRUE	TRUE
KIT	postLaser	KIT07	0.001018	110.46939	0.000343	-8.006857	TRUE	FALSE
KIC	preLaser	KIC15	0.002312	28.10000	0.000913	-7.041515	TRUE	FALSE

===NORMALITY ASSUMPTION===

===HOMOGENEITY OF VARIANCE ASSUMPTION===

Measurement	df1	df2	statistic	p
postLaser	2	24	0.054	0.948
preLaser	2	24	0.521	0.600

Mixed ANOVA

Mixed ANOVA (Type III test)

Effect	DFn	DFd	F	p	p..05	ges
Group	2	24	0.133000	0.876		9.0e-03
Measurement	1	24	0.000331	0.986		2.0e-06
Group:Measurement	2	24	1.875000	0.175		2.2e-02

Post-hoc Tests

===SIGNIFICANT TWO-WAY INTERACTION===

=PAIRWISE COMPARISONS BETWEEN GROUP LEVELS=

Measurement	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
postLaser	PeakLat	KIC	KIT	10	10	0.254	16.630	0.802	0.802	ns	0.114	negligible
postLaser	PeakLat	KIC	KIV	10	7	-1.005	14.903	0.331	0.662	ns	-0.476	small
postLaser	PeakLat	KIT	KIV	10	7	-1.489	14.351	0.158	0.474	ns	-0.723	moderate
preLaser	PeakLat	KIC	KIT	10	10	-0.173	17.545	0.864	1.000	ns	-0.077	negligible
preLaser	PeakLat	KIC	KIV	10	7	0.105	14.844	0.918	1.000	ns	0.050	negligible
preLaser	PeakLat	KIT	KIV	10	7	0.319	14.955	0.754	1.000	ns	0.153	negligible

=PAIRWISE COMPARISONS BETWEEN MEASUREMENTS AT EACH GROUP LEVELS=

Group	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
KIC	PeakLat	postLaser	preLaser	10	10	-0.490	9	0.636	0.636	ns	-0.155	negligible
KIT	PeakLat	postLaser	preLaser	10	10	-1.646	9	0.134	0.134	ns	-0.521	moderate
KIV	PeakLat	postLaser	preLaser	7	7	1.004	6	0.354	0.354	ns	0.379	small

===NON-SIGNIFICANT TWO-WAY INTERACTION===

=MAIN EFFECT OF MEASUREMENT=

.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif
PeakLat	postLaser	preLaser	27	27	-0.2831883	26	0.779	0.779	ns

=MAIN EFFECT OF GROUP=

.y.	group1	group2	n1	n2	p	p.signif	p.adj	p.adj.signif
PeakLat	KIC	KIT	20	20	0.965	ns	1	ns
PeakLat	KIC	KIV	20	14	0.555	ns	1	ns
PeakLat	KIT	KIV	20	14	0.529	ns	1	ns

Plots

RMS

Assumptions Check

===OUTLIERS===

Group	Measurement	Animal	PeakAmp	PeakLat	RMS	logRMS	is.outlier	is.extreme
KIC	postLaser	KIC11	0.002631	80.82979	0.001244	-6.905797	TRUE	FALSE
KIV	preLaser	KIV03	0.002418	77.20000	0.000971	-7.136828	TRUE	FALSE

===NORMALITY ASSUMPTION===

===HOMOGENEITY OF VARIANCE ASSUMPTION===

Measurement	df1	df2	statistic	p
postLaser	2	24	0.555	0.582
preLaser	2	24	1.902	0.171

Mixed ANOVA

Mixed ANOVA (Type III test)

Effect	DFn	DFd	F	p	p..05	ges
Group	2	24	0.330	0.722		2.6e-02
Measurement	1	24	0.005	0.946		7.9e-06
Group:Measurement	2	24	6.746	0.005		2.2e-02

Post-hoc Tests

===SIGNIFICANT TWO-WAY INTERACTION===

=PAIRWISE COMPARISONS BETWEEN GROUP LEVELS=

Measurement	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
postLaser	RMS	KIC	KIT	10	10	1.099	17.977	0.286	0.609	ns	0.492	small
postLaser	RMS	KIC	KIV	10	7	-0.066	14.816	0.948	0.948	ns	-0.031	negligible
postLaser	RMS	KIT	KIV	10	7	-1.332	14.910	0.203	0.609	ns	-0.631	moderate
preLaser	RMS	KIC	KIT	10	10	-0.217	16.590	0.831	1.000	ns	-0.097	negligible
preLaser	RMS	KIC	KIV	10	7	-0.843	14.753	0.413	1.000	ns	-0.398	small
preLaser	RMS	KIT	KIV	10	7	-0.439	13.176	0.668	1.000	ns	-0.203	small

=PAIRWISE COMPARISONS BETWEEN MEASUREMENTS AT EACH GROUP LEVELS=

Group	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
KIC	RMS	postLaser	preLaser	10	10	2.526	9	0.032	0.032		0.799	moderate
KIT	RMS	postLaser	preLaser	10	10	-2.586	9	0.029	0.029		-0.818	large
KIV	RMS	postLaser	preLaser	7	7	0.225	6	0.830	0.830	ns	0.085	negligible

===NON-SIGNIFICANT TWO-WAY INTERACTION===

=MAIN EFFECT OF MEASUREMENT=

.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif
RMS	postLaser	preLaser	27	27	-0.1038207	26	0.918	0.918	ns

=MAIN EFFECT OF GROUP=

.y.	group1	group2	n1	n2	p	p.signif	p.adj	p.adj.signif
RMS	KIC	KIT	20	20	0.480	ns	0.960	ns
RMS	KIC	KIV	20	14	0.634	ns	0.960	ns
RMS	KIT	KIV	20	14	0.266	ns	0.798	ns

Plots

logRMS

Assumptions Check

===OUTLIERS===

No outliers were detected.

===NORMALITY ASSUMPTION===

===HOMOGENEITY OF VARIANCE ASSUMPTION===

Measurement	df1	df2	statistic	p
postLaser	2	24	0.438	0.650
preLaser	2	24	1.579	0.227

Mixed ANOVA

Mixed ANOVA (Type III test)

Effect	DFn	DFd	F	p	p..05	ges
Group	2	24	0.803	0.459		6.0e-02
Measurement	1	24	0.002	0.962		4.4e-06
Group:Measurement	2	24	3.754	0.038		1.4e-02

Post-hoc Tests

===SIGNIFICANT TWO-WAY INTERACTION===

=PAIRWISE COMPARISONS BETWEEN GROUP LEVELS=

Measurement	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
postLaser	logRMS	KIC	KIT	10	10	1.244	18.000	0.230	0.460	ns	0.556	moderate
postLaser	logRMS	KIC	KIV	10	7	-0.358	14.998	0.726	0.726	ns	-0.170	negligible
postLaser	logRMS	KIT	KIV	10	7	-1.727	14.997	0.105	0.315	ns	-0.822	large
preLaser	logRMS	KIC	KIT	10	10	0.186	17.862	0.855	0.951	ns	0.083	negligible
preLaser	logRMS	KIC	KIV	10	7	-0.873	13.621	0.398	0.951	ns	-0.406	small
preLaser	logRMS	KIT	KIV	10	7	-1.039	13.094	0.317	0.951	ns	-0.480	small

=PAIRWISE COMPARISONS BETWEEN MEASUREMENTS AT EACH GROUP LEVELS=

Group	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
KIC	logRMS	postLaser	preLaser	10	10	2.544	9	0.032	0.032		0.805	large
KIT	logRMS	postLaser	preLaser	10	10	-1.773	9	0.110	0.110	ns	-0.561	moderate
KIV	logRMS	postLaser	preLaser	7	7	0.344	6	0.743	0.743	ns	0.130	negligible

===NON-SIGNIFICANT TWO-WAY INTERACTION===

=MAIN EFFECT OF MEASUREMENT=

.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif
logRMS	postLaser	preLaser	27	27	-0.0263545	26	0.979	0.979	ns

=MAIN EFFECT OF GROUP=

.y.	group1	group2	n1	n2	p	p.signif	p.adj	p.adj.signif
logRMS	KIC	KIT	20	20	0.262	ns	0.524	ns
logRMS	KIC	KIV	20	14	0.474	ns	0.524	ns
logRMS	KIT	KIV	20	14	0.086	ns	0.258	ns

Plots

10 Hz Frequency

All Layer

PeakAmp

Assumptions Check

===OUTLIERS===

Group	Measurement	Animal	PeakAmp	PeakLat	RMS	logRMS	is.outlier	is.extreme
KIC	preLaser	KIC04	0.005967	50.93333	0.003197	-5.782533	TRUE	TRUE

===NORMALITY ASSUMPTION===

===HOMOGENEITY OF VARIANCE ASSUMPTION===

Measurement	df1	df2	statistic	p
postLaser	2	24	0.619	0.547
preLaser	2	24	0.147	0.864

Mixed ANOVA

Mixed ANOVA (Type III test)

Effect	DFn	DFd	F	p	p..05	ges
Group	2	24	2.653	0.091		0.144
Measurement	1	24	0.122	0.730		0.001
Group:Measurement	2	24	0.665	0.524		0.013

Post-hoc Tests

===SIGNIFICANT TWO-WAY INTERACTION===

=PAIRWISE COMPARISONS BETWEEN GROUP LEVELS=

Measurement	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
postLaser	PeakAmp	KIC	KIT	10	10	2.886	16.034	0.011	0.032		1.291	large
postLaser	PeakAmp	KIC	KIV	10	7	0.467	13.557	0.648	0.648	ns	0.229	small
postLaser	PeakAmp	KIT	KIV	10	7	-2.122	10.407	0.059	0.118	ns	-1.073	large
preLaser	PeakAmp	KIC	KIT	10	10	1.163	12.807	0.266	0.675	ns	0.520	moderate
preLaser	PeakAmp	KIC	KIV	10	7	0.160	14.711	0.875	0.875	ns	0.076	negligible
preLaser	PeakAmp	KIT	KIV	10	7	-1.286	11.093	0.225	0.675	ns	-0.646	moderate

=PAIRWISE COMPARISONS BETWEEN MEASUREMENTS AT EACH GROUP LEVELS=

Group	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
KIC	PeakAmp	postLaser	preLaser	10	10	0.603	9	0.561	0.561	ns	0.191	negligible
KIT	PeakAmp	postLaser	preLaser	10	10	-1.894	9	0.091	0.091	ns	-0.599	moderate
KIV	PeakAmp	postLaser	preLaser	7	7	0.511	6	0.628	0.628	ns	0.193	negligible

===NON-SIGNIFICANT TWO-WAY INTERACTION===

=MAIN EFFECT OF MEASUREMENT=

.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif
PeakAmp	postLaser	preLaser	27	27	0.3121775	26	0.757	0.757	ns

=MAIN EFFECT OF GROUP=

.y.	group1	group2	n1	n2	p	p.signif	p.adj	p.adj.signif
PeakAmp	KIC	KIT	20	20	0.00776	**	0.0233
PeakAmp	KIC	KIV	20	14	0.64700	ns	0.6470	ns
PeakAmp	KIT	KIV	20	14	0.04510		0.0901	ns

Plots

PeakLat

Assumptions Check

===OUTLIERS===

Group	Measurement	Animal	PeakAmp	PeakLat	RMS	logRMS	is.outlier	is.extreme
KIT	postLaser	KIT03	0.002429	34.04	0.001222	-6.727343	TRUE	FALSE

===NORMALITY ASSUMPTION===

===HOMOGENEITY OF VARIANCE ASSUMPTION===

Measurement	df1	df2	statistic	p
postLaser	2	24	1.260	0.302
preLaser	2	24	1.218	0.313

Mixed ANOVA

Mixed ANOVA (Type III test)

Effect	DFn	DFd	F	p	p..05	ges
Group	2	24	0.860	0.436		0.063
Measurement	1	24	3.937	0.059		0.011
Group:Measurement	2	24	0.650	0.531		0.004

Post-hoc Tests

===SIGNIFICANT TWO-WAY INTERACTION===

=PAIRWISE COMPARISONS BETWEEN GROUP LEVELS=

Measurement	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
postLaser	PeakLat	KIC	KIT	10	10	-0.528	14.894	0.605	1.000	ns	-0.236	small
postLaser	PeakLat	KIC	KIV	10	7	-0.841	10.724	0.419	1.000	ns	-0.424	small
postLaser	PeakLat	KIT	KIV	10	7	-0.561	7.852	0.591	1.000	ns	-0.292	small
preLaser	PeakLat	KIC	KIT	10	10	-0.746	17.043	0.466	0.902	ns	-0.333	small
preLaser	PeakLat	KIC	KIV	10	7	-1.221	9.828	0.251	0.753	ns	-0.621	moderate
preLaser	PeakLat	KIT	KIV	10	7	-0.790	8.413	0.451	0.902	ns	-0.408	small

=PAIRWISE COMPARISONS BETWEEN MEASUREMENTS AT EACH GROUP LEVELS=

Group	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
KIC	PeakLat	postLaser	preLaser	10	10	-0.472	9	0.648	0.648	ns	-0.149	negligible
KIT	PeakLat	postLaser	preLaser	10	10	-1.058	9	0.318	0.318	ns	-0.335	small
KIV	PeakLat	postLaser	preLaser	7	7	-1.606	6	0.159	0.159	ns	-0.607	moderate

===NON-SIGNIFICANT TWO-WAY INTERACTION===

=MAIN EFFECT OF MEASUREMENT=

.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif
PeakLat	postLaser	preLaser	27	27	-1.861074	26	0.074	0.074	ns

=MAIN EFFECT OF GROUP=

.y.	group1	group2	n1	n2	p	p.signif	p.adj	p.adj.signif
PeakLat	KIC	KIT	20	20	0.4360	ns	0.536	ns
PeakLat	KIC	KIV	20	14	0.0726	ns	0.218	ns
PeakLat	KIT	KIV	20	14	0.2680	ns	0.536	ns

Plots

RMS

Assumptions Check

===OUTLIERS===

Group	Measurement	Animal	PeakAmp	PeakLat	RMS	logRMS	is.outlier	is.extreme
KIC	preLaser	KIC04	0.005967	50.93333	0.003197	-5.782533	TRUE	TRUE

===NORMALITY ASSUMPTION===

===HOMOGENEITY OF VARIANCE ASSUMPTION===

Measurement	df1	df2	statistic	p
postLaser	2	24	0.720	0.497
preLaser	2	24	0.063	0.939

Mixed ANOVA

Mixed ANOVA (Type III test)

Effect	DFn	DFd	F	p	p..05	ges
Group	2	24	2.120	0.142		0.122
Measurement	1	24	0.442	0.512		0.004
Group:Measurement	2	24	1.057	0.363		0.018

Post-hoc Tests

===SIGNIFICANT TWO-WAY INTERACTION===

=PAIRWISE COMPARISONS BETWEEN GROUP LEVELS=

Measurement	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
postLaser	RMS	KIC	KIT	10	10	2.627	16.812	0.018	0.053	ns	1.175	large
postLaser	RMS	KIC	KIV	10	7	0.101	10.959	0.922	0.922	ns	0.051	negligible
postLaser	RMS	KIT	KIV	10	7	-1.836	9.018	0.099	0.199	ns	-0.942	large
preLaser	RMS	KIC	KIT	10	10	0.947	13.744	0.360	0.774	ns	0.424	small
preLaser	RMS	KIC	KIV	10	7	-0.010	14.873	0.992	0.992	ns	-0.005	negligible
preLaser	RMS	KIT	KIV	10	7	-1.189	11.730	0.258	0.774	ns	-0.593	moderate

=PAIRWISE COMPARISONS BETWEEN MEASUREMENTS AT EACH GROUP LEVELS=

Group	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
KIC	RMS	postLaser	preLaser	10	10	0.815	9	0.436	0.436	ns	0.258	small
KIT	RMS	postLaser	preLaser	10	10	-2.532	9	0.032	0.032		-0.801	large
KIV	RMS	postLaser	preLaser	7	7	0.747	6	0.483	0.483	ns	0.282	small

===NON-SIGNIFICANT TWO-WAY INTERACTION===

=MAIN EFFECT OF MEASUREMENT=

.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif
RMS	postLaser	preLaser	27	27	0.5823871	26	0.565	0.565	ns

=MAIN EFFECT OF GROUP=

.y.	group1	group2	n1	n2	p	p.signif	p.adj	p.adj.signif
RMS	KIC	KIT	20	20	0.0211		0.0634	ns
RMS	KIC	KIV	20	14	0.9410	ns	0.9410	ns
RMS	KIT	KIV	20	14	0.0421		0.0843	ns

Plots

logRMS

Assumptions Check

===OUTLIERS===

Group	Measurement	Animal	PeakAmp	PeakLat	RMS	logRMS	is.outlier	is.extreme
KIC	postLaser	KIC10	0.000694	50.12500	0.000432	-7.766069	TRUE	FALSE
KIC	preLaser	KIC04	0.005967	50.93333	0.003197	-5.782533	TRUE	TRUE
KIC	preLaser	KIC10	0.000887	47.69231	0.000582	-7.480096	TRUE	FALSE

===NORMALITY ASSUMPTION===

===HOMOGENEITY OF VARIANCE ASSUMPTION===

Measurement	df1	df2	statistic	p
postLaser	2	24	0.165	0.849
preLaser	2	24	0.157	0.855

Mixed ANOVA

Mixed ANOVA (Type III test)

Effect	DFn	DFd	F	p	p..05	ges
Group	2	24	2.139	0.140		0.136000
Measurement	1	24	0.153	0.699		0.000742
Group:Measurement	2	24	2.611	0.094		0.025000

Post-hoc Tests

===SIGNIFICANT TWO-WAY INTERACTION===

=PAIRWISE COMPARISONS BETWEEN GROUP LEVELS=

Measurement	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
postLaser	logRMS	KIC	KIT	10	10	2.393	17.910	0.028	0.084	ns	1.070	large
postLaser	logRMS	KIC	KIV	10	7	0.250	12.737	0.806	0.806	ns	0.124	negligible
postLaser	logRMS	KIT	KIV	10	7	-1.896	13.352	0.080	0.160	ns	-0.932	large
preLaser	logRMS	KIC	KIT	10	10	0.987	17.955	0.337	0.690	ns	0.441	small
preLaser	logRMS	KIC	KIV	10	7	-0.189	14.941	0.852	0.852	ns	-0.091	negligible
preLaser	logRMS	KIT	KIV	10	7	-1.251	14.811	0.230	0.690	ns	-0.602	moderate

=PAIRWISE COMPARISONS BETWEEN MEASUREMENTS AT EACH GROUP LEVELS=

Group	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
KIC	logRMS	postLaser	preLaser	10	10	1.227	9	0.251	0.251	ns	0.388	small
KIT	logRMS	postLaser	preLaser	10	10	-2.746	9	0.023	0.023		-0.868	large
KIV	logRMS	postLaser	preLaser	7	7	0.574	6	0.587	0.587	ns	0.217	small

===NON-SIGNIFICANT TWO-WAY INTERACTION===

=MAIN EFFECT OF MEASUREMENT=

.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif
logRMS	postLaser	preLaser	27	27	0.3039498	26	0.764	0.764	ns

=MAIN EFFECT OF GROUP=

.y.	group1	group2	n1	n2	p	p.signif	p.adj	p.adj.signif
logRMS	KIC	KIT	20	20	0.0148		0.0443
logRMS	KIC	KIV	20	14	0.9460	ns	0.9460	ns
logRMS	KIT	KIV	20	14	0.0307		0.0615	ns

Plots

I_II Layer

PeakAmp

Assumptions Check

===OUTLIERS===

Group	Measurement	Animal	PeakAmp	PeakLat	RMS	logRMS	is.outlier	is.extreme
KIC	postLaser	KIC02	0.005139	50.93878	0.002488	-6.129194	TRUE	TRUE
KIT	postLaser	KIT03	0.003582	44.67347	0.001489	-6.649383	TRUE	TRUE
KIC	preLaser	KIC04	0.008923	57.36667	0.004140	-5.594033	TRUE	TRUE

===NORMALITY ASSUMPTION===

===HOMOGENEITY OF VARIANCE ASSUMPTION===

Measurement	df1	df2	statistic	p
postLaser	2	23	2.583	0.097
preLaser	2	23	1.394	0.268

Mixed ANOVA

Mixed ANOVA (Type III test)

Effect	DFn	DFd	F	p	p..05	ges
Group	2	23	2.398	0.113		0.138
Measurement	1	23	0.241	0.628		0.002
Group:Measurement	2	23	0.113	0.894		0.002

Post-hoc Tests

===SIGNIFICANT TWO-WAY INTERACTION===

=PAIRWISE COMPARISONS BETWEEN GROUP LEVELS=

Measurement	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
postLaser	PeakAmp	KIC	KIT	10	9	1.099	15.997	0.288	0.484	ns	0.500	moderate
postLaser	PeakAmp	KIC	KIV	10	7	-1.255	8.607	0.242	0.484	ns	-0.647	moderate
postLaser	PeakAmp	KIT	KIV	9	7	-1.910	7.376	0.096	0.287	ns	-1.006	large
preLaser	PeakAmp	KIC	KIT	10	9	1.041	9.941	0.323	0.646	ns	0.467	small
preLaser	PeakAmp	KIC	KIV	10	7	-0.837	9.895	0.422	0.646	ns	-0.425	small
preLaser	PeakAmp	KIT	KIV	9	7	-1.565	6.209	0.167	0.501	ns	-0.834	large

=PAIRWISE COMPARISONS BETWEEN MEASUREMENTS AT EACH GROUP LEVELS=

Group	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
KIC	PeakAmp	postLaser	preLaser	10	10	-0.286	9	0.781	0.781	ns	-0.090	negligible
KIT	PeakAmp	postLaser	preLaser	9	9	0.252	8	0.807	0.807	ns	0.084	negligible
KIV	PeakAmp	postLaser	preLaser	7	7	-0.580	6	0.583	0.583	ns	-0.219	small

===NON-SIGNIFICANT TWO-WAY INTERACTION===

=MAIN EFFECT OF MEASUREMENT=

.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif
PeakAmp	postLaser	preLaser	26	26	-0.4794228	25	0.636	0.636	ns

=MAIN EFFECT OF GROUP=

.y.	group1	group2	n1	n2	p	p.signif	p.adj	p.adj.signif
PeakAmp	KIC	KIT	20	18	0.28500	ns	0.2850	ns
PeakAmp	KIC	KIV	20	14	0.07330	ns	0.1470	ns
PeakAmp	KIT	KIV	18	14	0.00777	**	0.0233

Plots

PeakLat

Assumptions Check

===OUTLIERS===

Group	Measurement	Animal	PeakAmp	PeakLat	RMS	logRMS	is.outlier	is.extreme
KIT	postLaser	KIT08	0.001033	35.02273	0.000524	-7.689195	TRUE	FALSE
KIV	postLaser	KIV04	0.006123	66.61225	0.002932	-6.030379	TRUE	FALSE
KIV	postLaser	KIV12	0.001547	35.97959	0.000591	-7.484659	TRUE	FALSE
KIT	preLaser	KIT05	0.001156	38.53571	0.000523	-7.674206	TRUE	FALSE
KIT	preLaser	KIT08	0.001672	29.76000	0.001060	-7.159125	TRUE	TRUE
KIV	preLaser	KIV04	0.008073	71.66667	0.003736	-5.798269	TRUE	TRUE

===NORMALITY ASSUMPTION===

===HOMOGENEITY OF VARIANCE ASSUMPTION===

Measurement	df1	df2	statistic	p
postLaser	2	23	0.192	0.827
preLaser	2	23	0.282	0.757

Mixed ANOVA

Mixed ANOVA (Type III test)

Effect	DFn	DFd	F	p	p..05	ges
Group	2	23	1.444	0.257		0.093
Measurement	1	23	2.165	0.155		0.017
Group:Measurement	2	23	2.182	0.136		0.034

Post-hoc Tests

===SIGNIFICANT TWO-WAY INTERACTION===

=PAIRWISE COMPARISONS BETWEEN GROUP LEVELS=

Measurement	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
postLaser	PeakLat	KIC	KIT	10	9	0.053	16.866	0.958	1.000	ns	0.024	negligible
postLaser	PeakLat	KIC	KIV	10	7	-0.951	9.689	0.365	1.000	ns	-0.484	small
postLaser	PeakLat	KIT	KIV	9	7	-0.980	9.816	0.350	1.000	ns	-0.506	moderate
preLaser	PeakLat	KIC	KIT	10	9	2.024	16.978	0.059	0.177	ns	0.928	large
preLaser	PeakLat	KIC	KIV	10	7	-0.230	10.302	0.822	0.822	ns	-0.117	negligible
preLaser	PeakLat	KIT	KIV	9	7	-1.709	10.035	0.118	0.236	ns	-0.881	large

=PAIRWISE COMPARISONS BETWEEN MEASUREMENTS AT EACH GROUP LEVELS=

Group	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
KIC	PeakLat	postLaser	preLaser	10	10	-1.853	9	0.097	0.097	ns	-0.586	moderate
KIT	PeakLat	postLaser	preLaser	9	9	0.645	8	0.537	0.537	ns	0.215	small
KIV	PeakLat	postLaser	preLaser	7	7	-2.070	6	0.084	0.084	ns	-0.783	moderate

===NON-SIGNIFICANT TWO-WAY INTERACTION===

=MAIN EFFECT OF MEASUREMENT=

.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif
PeakLat	postLaser	preLaser	26	26	-1.501795	25	0.146	0.146	ns

=MAIN EFFECT OF GROUP=

.y.	group1	group2	n1	n2	p	p.signif	p.adj	p.adj.signif
PeakLat	KIC	KIT	20	18	0.1850	ns	0.369	ns
PeakLat	KIC	KIV	20	14	0.3510	ns	0.369	ns
PeakLat	KIT	KIV	18	14	0.0367		0.110	ns

Plots

RMS

Assumptions Check

===OUTLIERS===

Group	Measurement	Animal	PeakAmp	PeakLat	RMS	logRMS	is.outlier	is.extreme
KIC	postLaser	KIC02	0.005139	50.93878	0.002488	-6.129194	TRUE	FALSE
KIT	postLaser	KIT03	0.003582	44.67347	0.001489	-6.649383	TRUE	FALSE
KIC	preLaser	KIC04	0.008923	57.36667	0.004140	-5.594033	TRUE	TRUE

===NORMALITY ASSUMPTION===

===HOMOGENEITY OF VARIANCE ASSUMPTION===

Measurement	df1	df2	statistic	p
postLaser	2	23	3.252	0.057
preLaser	2	23	1.610	0.222

Mixed ANOVA

Mixed ANOVA (Type III test)

Effect	DFn	DFd	F	p	p..05	ges
Group	2	23	2.635	0.093		0.153000
Measurement	1	23	0.229	0.637		0.002000
Group:Measurement	2	23	0.048	0.953		0.000881

Post-hoc Tests

===SIGNIFICANT TWO-WAY INTERACTION===

=PAIRWISE COMPARISONS BETWEEN GROUP LEVELS=

Measurement	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
postLaser	RMS	KIC	KIT	10	9	1.913	14.481	0.076	0.223	ns	0.867	large
postLaser	RMS	KIC	KIV	10	7	-1.012	8.723	0.339	0.339	ns	-0.521	moderate
postLaser	RMS	KIT	KIV	9	7	-2.097	6.971	0.074	0.223	ns	-1.109	large
preLaser	RMS	KIC	KIT	10	9	1.242	10.385	0.242	0.489	ns	0.557	moderate
preLaser	RMS	KIC	KIV	10	7	-0.792	9.499	0.448	0.489	ns	-0.404	small
preLaser	RMS	KIT	KIV	9	7	-1.582	6.275	0.163	0.489	ns	-0.843	large

=PAIRWISE COMPARISONS BETWEEN MEASUREMENTS AT EACH GROUP LEVELS=

Group	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
KIC	RMS	postLaser	preLaser	10	10	-0.132	9	0.898	0.898	ns	-0.042	negligible
KIT	RMS	postLaser	preLaser	9	9	-0.332	8	0.748	0.748	ns	-0.111	negligible
KIV	RMS	postLaser	preLaser	7	7	-0.475	6	0.652	0.652	ns	-0.179	negligible

===NON-SIGNIFICANT TWO-WAY INTERACTION===

=MAIN EFFECT OF MEASUREMENT=

.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif
RMS	postLaser	preLaser	26	26	-0.4581887	25	0.651	0.651	ns

=MAIN EFFECT OF GROUP=

.y.	group1	group2	n1	n2	p	p.signif	p.adj	p.adj.signif
RMS	KIC	KIT	20	18	0.14000	ns	0.2140	ns
RMS	KIC	KIV	20	14	0.10700	ns	0.2140	ns
RMS	KIT	KIV	18	14	0.00457	**	0.0137

Plots

logRMS

Assumptions Check

===OUTLIERS===

Group	Measurement	Animal	PeakAmp	PeakLat	RMS	logRMS	is.outlier	is.extreme
KIC	postLaser	KIC02	0.005139	50.93878	0.002488	-6.129194	TRUE	FALSE
KIC	postLaser	KIC10	0.000750	35.41463	0.000314	-8.111476	TRUE	FALSE
KIC	preLaser	KIC04	0.008923	57.36667	0.004140	-5.594033	TRUE	FALSE

===NORMALITY ASSUMPTION===

===HOMOGENEITY OF VARIANCE ASSUMPTION===

Measurement	df1	df2	statistic	p
postLaser	2	23	1.221	0.313
preLaser	2	23	0.945	0.403

Mixed ANOVA

Mixed ANOVA (Type III test)

Effect	DFn	DFd	F	p	p..05	ges
Group	2	23	2.269	0.126		0.146000
Measurement	1	23	0.210	0.651		0.001000
Group:Measurement	2	23	0.061	0.941		0.000711

Post-hoc Tests

===SIGNIFICANT TWO-WAY INTERACTION===

=PAIRWISE COMPARISONS BETWEEN GROUP LEVELS=

Measurement	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
postLaser	logRMS	KIC	KIT	10	9	1.620	16.738	0.124	0.248	ns	0.740	moderate
postLaser	logRMS	KIC	KIV	10	7	-0.848	10.339	0.416	0.416	ns	-0.429	small
postLaser	logRMS	KIT	KIV	9	7	-2.032	9.027	0.073	0.218	ns	-1.056	large
preLaser	logRMS	KIC	KIT	10	9	1.201	14.400	0.249	0.498	ns	0.544	moderate
preLaser	logRMS	KIC	KIV	10	7	-0.686	11.245	0.507	0.507	ns	-0.344	small
preLaser	logRMS	KIT	KIV	9	7	-1.651	7.942	0.138	0.414	ns	-0.866	large

=PAIRWISE COMPARISONS BETWEEN MEASUREMENTS AT EACH GROUP LEVELS=

Group	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
KIC	logRMS	postLaser	preLaser	10	10	0.298	9	0.773	0.773	ns	0.094	negligible
KIT	logRMS	postLaser	preLaser	9	9	-0.022	8	0.983	0.983	ns	-0.007	negligible
KIV	logRMS	postLaser	preLaser	7	7	0.610	6	0.564	0.564	ns	0.231	small

===NON-SIGNIFICANT TWO-WAY INTERACTION===

=MAIN EFFECT OF MEASUREMENT=

.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif
logRMS	postLaser	preLaser	26	26	0.4621599	25	0.648	0.648	ns

=MAIN EFFECT OF GROUP=

.y.	group1	group2	n1	n2	p	p.signif	p.adj	p.adj.signif
logRMS	KIC	KIT	20	18	0.09060	ns	0.1810	ns
logRMS	KIC	KIV	20	14	0.19300	ns	0.1930	ns
logRMS	KIT	KIV	18	14	0.00615	**	0.0184

Plots

IV Layer

PeakAmp

Assumptions Check

===OUTLIERS===

Group	Measurement	Animal	PeakAmp	PeakLat	RMS	logRMS	is.outlier	is.extreme
KIC	preLaser	KIC04	0.011947	51.43333	0.006606	-5.066451	TRUE	TRUE
KIC	preLaser	KIC15	0.004839	35.21429	0.002261	-6.265758	TRUE	FALSE

===NORMALITY ASSUMPTION===

===HOMOGENEITY OF VARIANCE ASSUMPTION===

Measurement	df1	df2	statistic	p
postLaser	2	24	0.06	0.942
preLaser	2	24	0.05	0.951

Mixed ANOVA

Mixed ANOVA (Type III test)

Effect	DFn	DFd	F	p	p..05	ges
Group	2	24	0.883	0.426		0.058
Measurement	1	24	0.273	0.606		0.002
Group:Measurement	2	24	0.747	0.484		0.010

Post-hoc Tests

===SIGNIFICANT TWO-WAY INTERACTION===

=PAIRWISE COMPARISONS BETWEEN GROUP LEVELS=

Measurement	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
postLaser	PeakAmp	KIC	KIT	10	10	1.846	17.827	0.082	0.245	ns	0.826	large
postLaser	PeakAmp	KIC	KIV	10	7	0.298	12.081	0.771	0.771	ns	0.148	negligible
postLaser	PeakAmp	KIT	KIV	10	7	-1.235	11.209	0.242	0.484	ns	-0.620	moderate
preLaser	PeakAmp	KIC	KIT	10	10	0.512	14.002	0.617	1.000	ns	0.229	small
preLaser	PeakAmp	KIC	KIV	10	7	-0.410	14.088	0.688	1.000	ns	-0.200	negligible
preLaser	PeakAmp	KIT	KIV	10	7	-0.994	9.285	0.345	1.000	ns	-0.508	moderate

=PAIRWISE COMPARISONS BETWEEN MEASUREMENTS AT EACH GROUP LEVELS=

Group	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
KIC	PeakAmp	postLaser	preLaser	10	10	0.463	9	0.655	0.655	ns	0.146	negligible
KIT	PeakAmp	postLaser	preLaser	10	10	-1.682	9	0.127	0.127	ns	-0.532	moderate
KIV	PeakAmp	postLaser	preLaser	7	7	-1.750	6	0.131	0.131	ns	-0.661	moderate

===NON-SIGNIFICANT TWO-WAY INTERACTION===

=MAIN EFFECT OF MEASUREMENT=

.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif
PeakAmp	postLaser	preLaser	27	27	-0.4423199	26	0.662	0.662	ns

=MAIN EFFECT OF GROUP=

.y.	group1	group2	n1	n2	p	p.signif	p.adj	p.adj.signif
PeakAmp	KIC	KIT	20	20	0.147	ns	0.397	ns
PeakAmp	KIC	KIV	20	14	0.848	ns	0.848	ns
PeakAmp	KIT	KIV	20	14	0.132	ns	0.397	ns

Plots

PeakLat

Assumptions Check

===OUTLIERS===

Group	Measurement	Animal	PeakAmp	PeakLat	RMS	logRMS	is.outlier	is.extreme
KIC	postLaser	KIC03	0.006291	60.68889	0.003623	-5.871619	TRUE	FALSE

===NORMALITY ASSUMPTION===

===HOMOGENEITY OF VARIANCE ASSUMPTION===

Measurement	df1	df2	statistic	p
postLaser	2	24	0.912	0.415
preLaser	2	24	3.444	0.048

Mixed ANOVA

Mixed ANOVA (Type III test)

Effect	DFn	DFd	F	p	p..05	ges
Group	2	24	0.455	0.640		0.034000
Measurement	1	24	0.189	0.668		0.000622
Group:Measurement	2	24	0.371	0.694		0.002000

Post-hoc Tests

===SIGNIFICANT TWO-WAY INTERACTION===

=PAIRWISE COMPARISONS BETWEEN GROUP LEVELS=

Measurement	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
postLaser	PeakLat	KIC	KIT	10	10	-0.311	17.941	0.759	1	ns	-0.139	negligible
postLaser	PeakLat	KIC	KIV	10	7	-0.917	9.070	0.383	1	ns	-0.470	small
postLaser	PeakLat	KIT	KIV	10	7	-0.732	8.748	0.483	1	ns	-0.377	small
preLaser	PeakLat	KIC	KIT	10	10	-0.560	17.877	0.583	1	ns	-0.250	small
preLaser	PeakLat	KIC	KIV	10	7	-0.574	8.169	0.582	1	ns	-0.297	small
preLaser	PeakLat	KIT	KIV	10	7	-0.280	7.840	0.786	1	ns	-0.146	negligible

=PAIRWISE COMPARISONS BETWEEN MEASUREMENTS AT EACH GROUP LEVELS=

Group	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
KIC	PeakLat	postLaser	preLaser	10	10	0.100	9	0.923	0.923	ns	0.032	negligible
KIT	PeakLat	postLaser	preLaser	10	10	-0.424	9	0.682	0.682	ns	-0.134	negligible
KIV	PeakLat	postLaser	preLaser	7	7	0.961	6	0.373	0.373	ns	0.363	small

===NON-SIGNIFICANT TWO-WAY INTERACTION===

=MAIN EFFECT OF MEASUREMENT=

.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif
PeakLat	postLaser	preLaser	27	27	0.31365	26	0.756	0.756	ns

=MAIN EFFECT OF GROUP=

.y.	group1	group2	n1	n2	p	p.signif	p.adj	p.adj.signif
PeakLat	KIC	KIT	20	20	0.624	ns	0.766	ns
PeakLat	KIC	KIV	20	14	0.190	ns	0.571	ns
PeakLat	KIT	KIV	20	14	0.383	ns	0.766	ns

Plots

RMS

Assumptions Check

===OUTLIERS===

Group	Measurement	Animal	PeakAmp	PeakLat	RMS	logRMS	is.outlier	is.extreme
KIC	preLaser	KIC04	0.011947	51.43333	0.006606	-5.066451	TRUE	TRUE
KIC	preLaser	KIC15	0.004839	35.21429	0.002261	-6.265758	TRUE	FALSE

===NORMALITY ASSUMPTION===

===HOMOGENEITY OF VARIANCE ASSUMPTION===

Measurement	df1	df2	statistic	p
postLaser	2	24	0.259	0.774
preLaser	2	24	0.045	0.956

Mixed ANOVA

Mixed ANOVA (Type III test)

Effect	DFn	DFd	F	p	p..05	ges
Group	2	24	0.946	0.402		0.061000
Measurement	1	24	0.084	0.775		0.000608
Group:Measurement	2	24	0.588	0.563		0.008000

Post-hoc Tests

===SIGNIFICANT TWO-WAY INTERACTION===

=PAIRWISE COMPARISONS BETWEEN GROUP LEVELS=

Measurement	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
postLaser	RMS	KIC	KIT	10	10	1.892	16.749	0.076	0.228	ns	0.846	large
postLaser	RMS	KIC	KIV	10	7	0.209	12.541	0.838	0.838	ns	0.103	negligible
postLaser	RMS	KIT	KIV	10	7	-1.377	10.155	0.198	0.396	ns	-0.698	moderate
preLaser	RMS	KIC	KIT	10	10	0.547	13.243	0.593	1.000	ns	0.245	small
preLaser	RMS	KIC	KIV	10	7	-0.328	14.559	0.748	1.000	ns	-0.159	negligible
preLaser	RMS	KIT	KIV	10	7	-0.978	9.181	0.353	1.000	ns	-0.501	moderate

=PAIRWISE COMPARISONS BETWEEN MEASUREMENTS AT EACH GROUP LEVELS=

Group	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
KIC	RMS	postLaser	preLaser	10	10	0.442	9	0.669	0.669	ns	0.140	negligible
KIT	RMS	postLaser	preLaser	10	10	-2.357	9	0.043	0.043		-0.745	moderate
KIV	RMS	postLaser	preLaser	7	7	-1.051	6	0.334	0.334	ns	-0.397	small

===NON-SIGNIFICANT TWO-WAY INTERACTION===

=MAIN EFFECT OF MEASUREMENT=

.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif
RMS	postLaser	preLaser	27	27	-0.2479259	26	0.806	0.806	ns

=MAIN EFFECT OF GROUP=

.y.	group1	group2	n1	n2	p	p.signif	p.adj	p.adj.signif
RMS	KIC	KIT	20	20	0.131	ns	0.376	ns
RMS	KIC	KIV	20	14	0.869	ns	0.869	ns
RMS	KIT	KIV	20	14	0.125	ns	0.376	ns

Plots

logRMS

Assumptions Check

===OUTLIERS===

Group	Measurement	Animal	PeakAmp	PeakLat	RMS	logRMS	is.outlier	is.extreme
KIC	preLaser	KIC04	0.011947	51.43333	0.006606	-5.066451	TRUE	FALSE

===NORMALITY ASSUMPTION===

===HOMOGENEITY OF VARIANCE ASSUMPTION===

Measurement	df1	df2	statistic	p
postLaser	2	24	0.255	0.777
preLaser	2	24	0.214	0.808

Mixed ANOVA

Mixed ANOVA (Type III test)

Effect	DFn	DFd	F	p	p..05	ges
Group	2	24	1.277	0.297		8.60e-02
Measurement	1	24	0.008	0.930		3.66e-05
Group:Measurement	2	24	2.597	0.095		2.30e-02

Post-hoc Tests

===SIGNIFICANT TWO-WAY INTERACTION===

=PAIRWISE COMPARISONS BETWEEN GROUP LEVELS=

Measurement	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
postLaser	logRMS	KIC	KIT	10	10	1.975	18.000	0.064	0.192	ns	0.883	large
postLaser	logRMS	KIC	KIV	10	7	0.382	14.339	0.708	0.708	ns	0.186	negligible
postLaser	logRMS	KIT	KIV	10	7	-1.582	14.341	0.135	0.270	ns	-0.768	moderate
preLaser	logRMS	KIC	KIT	10	10	0.524	17.558	0.607	1.000	ns	0.234	small
preLaser	logRMS	KIC	KIV	10	7	-0.580	14.718	0.571	1.000	ns	-0.280	small
preLaser	logRMS	KIT	KIV	10	7	-1.170	13.751	0.262	0.786	ns	-0.572	moderate

=PAIRWISE COMPARISONS BETWEEN MEASUREMENTS AT EACH GROUP LEVELS=

Group	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
KIC	logRMS	postLaser	preLaser	10	10	1.136	9	0.285	0.285	ns	0.359	small
KIT	logRMS	postLaser	preLaser	10	10	-2.595	9	0.029	0.029		-0.820	large
KIV	logRMS	postLaser	preLaser	7	7	-1.447	6	0.198	0.198	ns	-0.547	moderate

===NON-SIGNIFICANT TWO-WAY INTERACTION===

=MAIN EFFECT OF MEASUREMENT=

.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif
logRMS	postLaser	preLaser	27	27	-0.0159785	26	0.987	0.987	ns

=MAIN EFFECT OF GROUP=

.y.	group1	group2	n1	n2	p	p.signif	p.adj	p.adj.signif
logRMS	KIC	KIT	20	20	0.0686	ns	0.206	ns
logRMS	KIC	KIV	20	14	0.8780	ns	0.878	ns
logRMS	KIT	KIV	20	14	0.0713	ns	0.206	ns

Plots

V Layer

PeakAmp

Assumptions Check

===OUTLIERS===

Group	Measurement	Animal	PeakAmp	PeakLat	RMS	logRMS	is.outlier	is.extreme
KIC	postLaser	KIC10	0.000687	58.72727	0.000373	-7.960809	TRUE	FALSE
KIC	postLaser	KIC11	0.003678	40.16279	0.001785	-6.571590	TRUE	FALSE

===NORMALITY ASSUMPTION===

===HOMOGENEITY OF VARIANCE ASSUMPTION===

Measurement	df1	df2	statistic	p
postLaser	2	24	1.182	0.324
preLaser	2	24	0.260	0.773

Mixed ANOVA

Mixed ANOVA (Type III test)

Effect	DFn	DFd	F	p	p..05	ges
Group	2	24	2.245	0.128		0.139
Measurement	1	24	2.221	0.149		0.012
Group:Measurement	2	24	2.483	0.105		0.027

Post-hoc Tests

===SIGNIFICANT TWO-WAY INTERACTION===

=PAIRWISE COMPARISONS BETWEEN GROUP LEVELS=

Measurement	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
postLaser	PeakAmp	KIC	KIT	10	10	2.333	17.360	0.032	0.096	ns	1.043	large
postLaser	PeakAmp	KIC	KIV	10	7	-0.432	10.649	0.675	0.675	ns	-0.218	small
postLaser	PeakAmp	KIT	KIV	10	7	-2.152	9.259	0.059	0.118	ns	-1.101	large
preLaser	PeakAmp	KIC	KIT	10	10	0.725	17.996	0.478	0.956	ns	0.324	small
preLaser	PeakAmp	KIC	KIV	10	7	-0.653	10.746	0.527	0.956	ns	-0.329	small
preLaser	PeakAmp	KIT	KIV	10	7	-1.206	10.863	0.254	0.762	ns	-0.607	moderate

=PAIRWISE COMPARISONS BETWEEN MEASUREMENTS AT EACH GROUP LEVELS=

Group	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
KIC	PeakAmp	postLaser	preLaser	10	10	2.330	9	0.045	0.045		0.737	moderate
KIT	PeakAmp	postLaser	preLaser	10	10	-1.094	9	0.302	0.302	ns	-0.346	small
KIV	PeakAmp	postLaser	preLaser	7	7	1.039	6	0.339	0.339	ns	0.393	small

===NON-SIGNIFICANT TWO-WAY INTERACTION===

=MAIN EFFECT OF MEASUREMENT=

.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif
PeakAmp	postLaser	preLaser	27	27	1.301495	26	0.205	0.205	ns

=MAIN EFFECT OF GROUP=

.y.	group1	group2	n1	n2	p	p.signif	p.adj	p.adj.signif
PeakAmp	KIC	KIT	20	20	0.05050	ns	0.1010	ns
PeakAmp	KIC	KIV	20	14	0.39300	ns	0.3930	ns
PeakAmp	KIT	KIV	20	14	0.00992	**	0.0298

Plots

PeakLat

Assumptions Check

===OUTLIERS===

Group	Measurement	Animal	PeakAmp	PeakLat	RMS	logRMS	is.outlier	is.extreme
KIC	postLaser	KIC03	0.002439	67.45652	0.001122	-6.991058	TRUE	FALSE
KIC	preLaser	KIC11	0.002896	38.11111	0.001332	-6.826848	TRUE	FALSE
KIC	preLaser	KIC16	0.002431	30.89655	0.000967	-7.060775	TRUE	TRUE

===NORMALITY ASSUMPTION===

===HOMOGENEITY OF VARIANCE ASSUMPTION===

Measurement	df1	df2	statistic	p
postLaser	2	24	0.534	0.593
preLaser	2	24	1.781	0.190

Mixed ANOVA

Mixed ANOVA (Type III test)

Effect	DFn	DFd	F	p	p..05	ges
Group	2	24	0.359	0.702		0.02700
Measurement	1	24	0.035	0.853		0.00012
Group:Measurement	2	24	0.735	0.490		0.00500

Post-hoc Tests

===SIGNIFICANT TWO-WAY INTERACTION===

=PAIRWISE COMPARISONS BETWEEN GROUP LEVELS=

Measurement	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
postLaser	PeakLat	KIC	KIT	10	10	0.038	14.874	0.970	1	ns	0.017	negligible
postLaser	PeakLat	KIC	KIV	10	7	-0.504	13.144	0.623	1	ns	-0.248	small
postLaser	PeakLat	KIT	KIV	10	7	-0.619	9.165	0.551	1	ns	-0.317	small
preLaser	PeakLat	KIC	KIT	10	10	-0.822	16.758	0.423	1	ns	-0.368	small
preLaser	PeakLat	KIC	KIV	10	7	-0.856	9.445	0.413	1	ns	-0.437	small
preLaser	PeakLat	KIT	KIV	10	7	-0.387	8.003	0.709	1	ns	-0.201	small

=PAIRWISE COMPARISONS BETWEEN MEASUREMENTS AT EACH GROUP LEVELS=

Group	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
KIC	PeakLat	postLaser	preLaser	10	10	0.653	9	0.530	0.530	ns	0.207	small
KIT	PeakLat	postLaser	preLaser	10	10	-1.206	9	0.258	0.258	ns	-0.381	small
KIV	PeakLat	postLaser	preLaser	7	7	-0.312	6	0.766	0.766	ns	-0.118	negligible

===NON-SIGNIFICANT TWO-WAY INTERACTION===

=MAIN EFFECT OF MEASUREMENT=

.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif
PeakLat	postLaser	preLaser	27	27	-0.1513566	26	0.881	0.881	ns

=MAIN EFFECT OF GROUP=

.y.	group1	group2	n1	n2	p	p.signif	p.adj	p.adj.signif
PeakLat	KIC	KIT	20	20	0.661	ns	0.882	ns
PeakLat	KIC	KIV	20	14	0.245	ns	0.735	ns
PeakLat	KIT	KIV	20	14	0.441	ns	0.882	ns

Plots

RMS

Assumptions Check

===OUTLIERS===

Group	Measurement	Animal	PeakAmp	PeakLat	RMS	logRMS	is.outlier	is.extreme
KIC	postLaser	KIC10	0.000687	58.72727	0.000373	-7.960809	TRUE	FALSE
KIC	postLaser	KIC11	0.003678	40.16279	0.001785	-6.571590	TRUE	FALSE

===NORMALITY ASSUMPTION===

===HOMOGENEITY OF VARIANCE ASSUMPTION===

Measurement	df1	df2	statistic	p
postLaser	2	24	2.481	0.105
preLaser	2	24	0.715	0.499

Mixed ANOVA

Mixed ANOVA (Type III test)

Effect	DFn	DFd	F	p	p..05	ges
Group	2	24	2.208	0.132		0.128
Measurement	1	24	4.777	0.039		0.039
Group:Measurement	2	24	1.768	0.192		0.029

Post-hoc Tests

===SIGNIFICANT TWO-WAY INTERACTION===

=PAIRWISE COMPARISONS BETWEEN GROUP LEVELS=

Measurement	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
postLaser	RMS	KIC	KIT	10	10	1.777	17.799	0.093	0.272	ns	0.795	moderate
postLaser	RMS	KIC	KIV	10	7	-0.838	8.815	0.424	0.424	ns	-0.431	small
postLaser	RMS	KIT	KIV	10	7	-1.913	8.285	0.091	0.272	ns	-0.990	large
preLaser	RMS	KIC	KIT	10	10	0.657	17.996	0.519	1.000	ns	0.294	small
preLaser	RMS	KIC	KIV	10	7	-0.657	9.414	0.527	1.000	ns	-0.335	small
preLaser	RMS	KIT	KIV	10	7	-1.096	9.323	0.301	0.903	ns	-0.560	moderate

=PAIRWISE COMPARISONS BETWEEN MEASUREMENTS AT EACH GROUP LEVELS=

Group	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
KIC	RMS	postLaser	preLaser	10	10	2.839	9	0.019	0.019		0.898	large
KIT	RMS	postLaser	preLaser	10	10	-0.262	9	0.799	0.799	ns	-0.083	negligible
KIV	RMS	postLaser	preLaser	7	7	1.343	6	0.228	0.228	ns	0.508	moderate

===NON-SIGNIFICANT TWO-WAY INTERACTION===

=MAIN EFFECT OF MEASUREMENT=

.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif
RMS	postLaser	preLaser	27	27	1.930309	26	0.064	0.064	ns

=MAIN EFFECT OF GROUP=

.y.	group1	group2	n1	n2	p	p.signif	p.adj	p.adj.signif
RMS	KIC	KIT	20	20	0.1450	ns	0.2910	ns
RMS	KIC	KIV	20	14	0.2040	ns	0.2910	ns
RMS	KIT	KIV	20	14	0.0113		0.0339

Plots

logRMS

Assumptions Check

===OUTLIERS===

Group	Measurement	Animal	PeakAmp	PeakLat	RMS	logRMS	is.outlier	is.extreme
KIC	postLaser	KIC10	0.000687	58.72727	0.000373	-7.960809	TRUE	TRUE
KIC	preLaser	KIC10	0.000833	53.68966	0.000437	-7.804805	TRUE	FALSE
KIT	preLaser	KIT07	0.000528	47.42105	0.000261	-8.313501	TRUE	FALSE

===NORMALITY ASSUMPTION===

===HOMOGENEITY OF VARIANCE ASSUMPTION===

Measurement	df1	df2	statistic	p
postLaser	2	24	0.694	0.510
preLaser	2	24	0.119	0.888

Mixed ANOVA

Mixed ANOVA (Type III test)

Effect	DFn	DFd	F	p	p..05	ges
Group	2	24	1.466	0.251		0.098
Measurement	1	24	3.064	0.093		0.014
Group:Measurement	2	24	1.813	0.185		0.016

Post-hoc Tests

===SIGNIFICANT TWO-WAY INTERACTION===

=PAIRWISE COMPARISONS BETWEEN GROUP LEVELS=

Measurement	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
postLaser	logRMS	KIC	KIT	10	10	1.591	17.141	0.130	0.312	ns	0.712	moderate
postLaser	logRMS	KIC	KIV	10	7	-0.465	11.200	0.651	0.651	ns	-0.233	small
postLaser	logRMS	KIT	KIV	10	7	-1.746	13.215	0.104	0.312	ns	-0.859	large
preLaser	logRMS	KIC	KIT	10	10	0.578	17.125	0.571	0.996	ns	0.259	small
preLaser	logRMS	KIC	KIV	10	7	-0.699	11.924	0.498	0.996	ns	-0.349	small
preLaser	logRMS	KIT	KIV	10	7	-1.139	13.891	0.274	0.822	ns	-0.556	moderate

=PAIRWISE COMPARISONS BETWEEN MEASUREMENTS AT EACH GROUP LEVELS=

Group	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
KIC	logRMS	postLaser	preLaser	10	10	2.347	9	0.044	0.044		0.742	moderate
KIT	logRMS	postLaser	preLaser	10	10	-0.486	9	0.638	0.638	ns	-0.154	negligible
KIV	logRMS	postLaser	preLaser	7	7	1.257	6	0.255	0.255	ns	0.475	small

===NON-SIGNIFICANT TWO-WAY INTERACTION===

=MAIN EFFECT OF MEASUREMENT=

.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif
logRMS	postLaser	preLaser	27	27	1.605502	26	0.12	0.12	ns

=MAIN EFFECT OF GROUP=

.y.	group1	group2	n1	n2	p	p.signif	p.adj	p.adj.signif
logRMS	KIC	KIT	20	20	0.1180	ns	0.2370	ns
logRMS	KIC	KIV	20	14	0.4320	ns	0.4320	ns
logRMS	KIT	KIV	20	14	0.0299		0.0897	ns

Plots

VI Layer

PeakAmp

Assumptions Check

===OUTLIERS===

Group	Measurement	Animal	PeakAmp	PeakLat	RMS	logRMS	is.outlier	is.extreme
KIC	postLaser	KIC15	0.003464	22.77551	0.001282	-6.737997	TRUE	TRUE
KIV	postLaser	KIV03	0.002691	50.77551	0.001389	-6.800322	TRUE	FALSE
KIC	preLaser	KIC15	0.002278	22.13333	0.000939	-7.044143	TRUE	FALSE
KIV	preLaser	KIV03	0.002355	46.58621	0.001037	-7.006632	TRUE	FALSE

===NORMALITY ASSUMPTION===

===HOMOGENEITY OF VARIANCE ASSUMPTION===

Measurement	df1	df2	statistic	p
postLaser	2	24	0.668	0.522
preLaser	2	24	0.144	0.867

Mixed ANOVA

Mixed ANOVA (Type III test)

Effect	DFn	DFd	F	p	p..05	ges
Group	2	24	1.037	0.370		0.074
Measurement	1	24	4.207	0.051		0.013
Group:Measurement	2	24	2.354	0.117		0.015

Post-hoc Tests

===SIGNIFICANT TWO-WAY INTERACTION===

=PAIRWISE COMPARISONS BETWEEN GROUP LEVELS=

Measurement	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
postLaser	PeakAmp	KIC	KIT	10	10	1.354	13.749	0.198	0.480	ns	0.606	moderate
postLaser	PeakAmp	KIC	KIV	10	7	-0.001	14.952	0.999	0.999	ns	0.000	negligible
postLaser	PeakAmp	KIT	KIV	10	7	-1.512	10.441	0.160	0.480	ns	-0.764	moderate
preLaser	PeakAmp	KIC	KIT	10	10	0.506	17.640	0.619	0.740	ns	0.226	small
preLaser	PeakAmp	KIC	KIV	10	7	-0.926	13.942	0.370	0.740	ns	-0.452	small
preLaser	PeakAmp	KIT	KIV	10	7	-1.470	12.759	0.166	0.498	ns	-0.727	moderate

=PAIRWISE COMPARISONS BETWEEN MEASUREMENTS AT EACH GROUP LEVELS=

Group	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
KIC	PeakAmp	postLaser	preLaser	10	10	2.309	9	0.046	0.046		0.730	moderate
KIT	PeakAmp	postLaser	preLaser	10	10	0.009	9	0.993	0.993	ns	0.003	negligible
KIV	PeakAmp	postLaser	preLaser	7	7	1.323	6	0.234	0.234	ns	0.500	small

===NON-SIGNIFICANT TWO-WAY INTERACTION===

=MAIN EFFECT OF MEASUREMENT=

.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif
PeakAmp	postLaser	preLaser	27	27	2.061004	26	0.049	0.049

=MAIN EFFECT OF GROUP=

.y.	group1	group2	n1	n2	p	p.signif	p.adj	p.adj.signif
PeakAmp	KIC	KIT	20	20	0.1540	ns	0.309	ns
PeakAmp	KIC	KIV	20	14	0.5780	ns	0.578	ns
PeakAmp	KIT	KIV	20	14	0.0669	ns	0.201	ns

Plots

PeakLat

Assumptions Check

===OUTLIERS===

Group	Measurement	Animal	PeakAmp	PeakLat	RMS	logRMS	is.outlier	is.extreme
KIC	postLaser	KIC15	0.003464	22.77551	0.001282	-6.737997	TRUE	FALSE
KIC	preLaser	KIC15	0.002278	22.13333	0.000939	-7.044143	TRUE	FALSE
KIC	preLaser	KIC16	0.001577	31.79310	0.000860	-7.194202	TRUE	FALSE

===NORMALITY ASSUMPTION===

===HOMOGENEITY OF VARIANCE ASSUMPTION===

Measurement	df1	df2	statistic	p
postLaser	2	24	1.104	0.348
preLaser	2	24	0.709	0.502

Mixed ANOVA

Mixed ANOVA (Type III test)

Effect	DFn	DFd	F	p	p..05	ges
Group	2	24	0.274	0.763		0.020000
Measurement	1	24	0.068	0.796		0.000277
Group:Measurement	2	24	0.083	0.920		0.000676

Post-hoc Tests

===SIGNIFICANT TWO-WAY INTERACTION===

=PAIRWISE COMPARISONS BETWEEN GROUP LEVELS=

Measurement	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
postLaser	PeakLat	KIC	KIT	10	10	0.237	13.487	0.817	1.000	ns	0.106	negligible
postLaser	PeakLat	KIC	KIV	10	7	-0.432	10.590	0.674	1.000	ns	-0.196	negligible
postLaser	PeakLat	KIT	KIV	10	7	-1.199	13.796	0.251	0.753	ns	-0.558	moderate
preLaser	PeakLat	KIC	KIT	10	10	0.164	15.254	0.872	1.000	ns	0.073	negligible
preLaser	PeakLat	KIC	KIV	10	7	-0.587	13.694	0.567	1.000	ns	-0.273	small
preLaser	PeakLat	KIT	KIV	10	7	-1.017	14.844	0.326	0.978	ns	-0.489	small

=PAIRWISE COMPARISONS BETWEEN MEASUREMENTS AT EACH GROUP LEVELS=

Group	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
KIC	PeakLat	postLaser	preLaser	10	10	0.453	9	0.661	0.661	ns	0.143	negligible
KIT	PeakLat	postLaser	preLaser	10	10	0.245	9	0.812	0.812	ns	0.077	negligible
KIV	PeakLat	postLaser	preLaser	7	7	-0.215	6	0.837	0.837	ns	-0.081	negligible

===NON-SIGNIFICANT TWO-WAY INTERACTION===

=MAIN EFFECT OF MEASUREMENT=

.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif
PeakLat	postLaser	preLaser	27	27	0.3450735	26	0.733	0.733	ns

=MAIN EFFECT OF GROUP=

.y.	group1	group2	n1	n2	p	p.signif	p.adj	p.adj.signif
PeakLat	KIC	KIT	20	20	0.751	ns	0.948	ns
PeakLat	KIC	KIV	20	14	0.473	ns	0.948	ns
PeakLat	KIT	KIV	20	14	0.316	ns	0.948	ns

Plots

RMS

Assumptions Check

===OUTLIERS===

Group	Measurement	Animal	PeakAmp	PeakLat	RMS	logRMS	is.outlier	is.extreme
KIV	postLaser	KIV03	0.002691	50.77551	0.001389	-6.800322	TRUE	FALSE

===NORMALITY ASSUMPTION===

===HOMOGENEITY OF VARIANCE ASSUMPTION===

Measurement	df1	df2	statistic	p
postLaser	2	24	0.232	0.795
preLaser	2	24	1.946	0.165

Mixed ANOVA

Mixed ANOVA (Type III test)

Effect	DFn	DFd	F	p	p..05	ges
Group	2	24	0.606	0.554		0.044
Measurement	1	24	2.392	0.135		0.009
Group:Measurement	2	24	1.894	0.172		0.014

Post-hoc Tests

===SIGNIFICANT TWO-WAY INTERACTION===

=PAIRWISE COMPARISONS BETWEEN GROUP LEVELS=

Measurement	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
postLaser	RMS	KIC	KIT	10	10	0.981	17.960	0.340	0.879	ns	0.439	small
postLaser	RMS	KIC	KIV	10	7	-0.165	13.695	0.871	0.879	ns	-0.081	negligible
postLaser	RMS	KIT	KIV	10	7	-1.095	13.314	0.293	0.879	ns	-0.538	moderate
preLaser	RMS	KIC	KIT	10	10	-0.011	17.379	0.992	0.992	ns	-0.005	negligible
preLaser	RMS	KIC	KIV	10	7	-1.344	14.817	0.199	0.597	ns	-0.635	moderate
preLaser	RMS	KIT	KIV	10	7	-1.164	13.938	0.264	0.597	ns	-0.543	moderate

=PAIRWISE COMPARISONS BETWEEN MEASUREMENTS AT EACH GROUP LEVELS=

Group	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
KIC	RMS	postLaser	preLaser	10	10	2.579	9	0.030	0.030		0.815	large
KIT	RMS	postLaser	preLaser	10	10	-0.183	9	0.859	0.859	ns	-0.058	negligible
KIV	RMS	postLaser	preLaser	7	7	0.468	6	0.656	0.656	ns	0.177	negligible

===NON-SIGNIFICANT TWO-WAY INTERACTION===

=MAIN EFFECT OF MEASUREMENT=

.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif
RMS	postLaser	preLaser	27	27	1.587597	26	0.124	0.124	ns

=MAIN EFFECT OF GROUP=

.y.	group1	group2	n1	n2	p	p.signif	p.adj	p.adj.signif
RMS	KIC	KIT	20	20	0.432	ns	0.864	ns
RMS	KIC	KIV	20	14	0.432	ns	0.864	ns
RMS	KIT	KIV	20	14	0.137	ns	0.411	ns

Plots

logRMS

Assumptions Check

===OUTLIERS===

No outliers were detected.

===NORMALITY ASSUMPTION===

===HOMOGENEITY OF VARIANCE ASSUMPTION===

Measurement	df1	df2	statistic	p
postLaser	2	24	0.531	0.595
preLaser	2	24	2.215	0.131

Mixed ANOVA

Mixed ANOVA (Type III test)

Effect	DFn	DFd	F	p	p..05	ges
Group	2	24	0.967	0.395		0.070
Measurement	1	24	1.464	0.238		0.004
Group:Measurement	2	24	2.299	0.122		0.012

Post-hoc Tests

===SIGNIFICANT TWO-WAY INTERACTION===

=PAIRWISE COMPARISONS BETWEEN GROUP LEVELS=

Measurement	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
postLaser	logRMS	KIC	KIT	10	10	0.930	17.943	0.365	0.730	ns	0.416	small
postLaser	logRMS	KIC	KIV	10	7	-0.289	14.996	0.777	0.777	ns	-0.138	negligible
postLaser	logRMS	KIT	KIV	10	7	-1.330	14.977	0.203	0.609	ns	-0.636	moderate
preLaser	logRMS	KIC	KIT	10	10	0.217	17.839	0.830	0.830	ns	0.097	negligible
preLaser	logRMS	KIC	KIV	10	7	-1.691	13.864	0.113	0.268	ns	-0.788	moderate
preLaser	logRMS	KIT	KIV	10	7	-1.832	13.300	0.089	0.268	ns	-0.848	large

=PAIRWISE COMPARISONS BETWEEN MEASUREMENTS AT EACH GROUP LEVELS=

Group	.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif	effsize	magnitude
KIC	logRMS	postLaser	preLaser	10	10	3.054	9	0.014	0.014		0.966	large
KIT	logRMS	postLaser	preLaser	10	10	0.142	9	0.890	0.890	ns	0.045	negligible
KIV	logRMS	postLaser	preLaser	7	7	-0.583	6	0.581	0.581	ns	-0.220	small

===NON-SIGNIFICANT TWO-WAY INTERACTION===

=MAIN EFFECT OF MEASUREMENT=

.y.	group1	group2	n1	n2	statistic	df	p	p.adj	p.adj.signif
logRMS	postLaser	preLaser	27	27	1.385696	26	0.178	0.178	ns

=MAIN EFFECT OF GROUP=

.y.	group1	group2	n1	n2	p	p.signif	p.adj	p.adj.signif
logRMS	KIC	KIT	20	20	0.3570	ns	0.550	ns
logRMS	KIC	KIV	20	14	0.2750	ns	0.550	ns
logRMS	KIT	KIV	20	14	0.0571	ns	0.171	ns

Plots