ROS probe H2DCFDA (H2-DCF, DCF)
- The cell-permeant 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (also known as dichlorofluores cin diacetate) is a chemically reduced form of fluorescein used as an indicator for reactive oxygen species (ROS)
- In cells, for example to detect the generation of reactive oxygen intermediates in neutrophils and macrophages.
- Upon cleavage of the acetate groups by intracellular esterases and oxidation, the nonfluorescent H2DCFDA is converted to the highly fluorescent
- 2’,7’-dichlorofluorescein (DCF). Versions of this indicator that have better cellular retention are available .
Spectrum
#Structure 
library(flowCore)
library(flowClust)
setwd("/Users/apple/Google Drive/presentations/mydistill/my_distill/jyotifacs/fcsonly")
fname=list.files(pattern="Tube")
i=1
ff=read.FCS(fname[i])
df=data.frame(ff@exprs[,1:4])
colnames(df)
[1] "FSC.A" "SSC.A" "FITC.A" "PE.A" FlowClust
[1] -171595.3 -170940.4
Rule of identifying outliers: 80% quantile