GRF-GIF phenotypic effect
AB
01/12/2020
Tiller number
##
## Dunnett's test for comparing several treatments with a control :
## 95% family-wise confidence level
##
## $`0`
## diff lwr.ci upr.ci pval
## 1-0 10.638298 8.0414773 13.235118 < 2e-16 ***
## 2-0 10.953846 8.3918319 13.515860 < 2e-16 ***
## 2_cadenza-0 3.000000 -0.2324425 6.232442 0.0759 .
## 3-0 11.828125 9.2646814 14.391569 < 2e-16 ***
## 4-0 7.580645 5.0142073 10.147083 3.7e-12 ***
## 5-0 8.642857 5.9626574 11.323057 6.1e-14 ***
## 6-0 8.000000 5.1239132 10.876087 6.2e-11 ***
## 7-0 10.000000 7.1716128 12.828387 8.9e-16 ***
##
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1Note: Tiller number is significantly increased in GRF lines
Plant Height
##
## Dunnett's test for comparing several treatments with a control :
## 95% family-wise confidence level
##
## $`0`
## diff lwr.ci upr.ci pval
## 1-0 -12.697234 -18.51219 -6.882281 3.4e-07 ***
## 2-0 -8.948462 -14.68547 -3.211449 0.00076 ***
## 2_cadenza-0 -10.010000 -17.24827 -2.771726 0.00346 **
## 3-0 -17.781875 -23.52209 -12.041662 3.7e-13 ***
## 4-0 -10.577742 -16.32466 -4.830824 2.3e-05 ***
## 5-0 -7.052857 -13.05452 -1.051197 0.01616 *
## 6-0 -19.210000 -25.65030 -12.769698 2.6e-12 ***
## 7-0 -21.710000 -28.04349 -15.376510 5.0e-15 ***
##
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1Note: Plant height seems to be reduced by GRF but only 1 plant measured in 0 copy!
Number of Spikes per plant
##
## Dunnett's test for comparing several treatments with a control :
## 95% family-wise confidence level
##
## $`0`
## diff lwr.ci upr.ci pval
## 1-0 10.638298 8.0663785 13.210217 < 2e-16 ***
## 2-0 10.953846 8.4163993 13.491293 < 2e-16 ***
## 2_cadenza-0 3.000000 -0.2014462 6.201446 0.0721 .
## 3-0 11.828125 9.2892626 14.366987 < 2e-16 ***
## 4-0 7.354839 4.8130107 9.896667 9.3e-12 ***
## 5-0 8.642857 5.9883582 11.297356 4.4e-14 ***
## 6-0 8.000000 5.1514923 10.848508 4.1e-11 ***
## 7-0 10.000000 7.1987345 12.801265 4.4e-16 ***
##
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1Note: Number of spikes is increased by GRF (which fits with increased tiller number). However, 0, 6 and 7 copy numbers were only one measurment
Spikelets per Spike
##
## Dunnett's test for comparing several treatments with a control :
## 95% family-wise confidence level
##
## $`0`
## diff lwr.ci upr.ci pval
## 1-0 -2.123404 -4.572609 0.3258003 0.1061
## 2-0 -2.661538 -5.077915 -0.2451616 0.0262 *
## 2_cadenza-0 4.600000 1.551305 7.6486946 0.0012 **
## 3-0 -2.650000 -5.067725 -0.2322751 0.0276 *
## 4-0 -1.254839 -3.675388 1.1657103 0.4766
## 5-0 -3.257143 -5.784987 -0.7292988 0.0075 **
## 6-0 1.314286 -1.398310 4.0268816 0.5326
## 7-0 -2.962500 -5.630108 -0.2948922 0.0250 *
##
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1Note: Number of spikelets per spike is increased in cadenza (genotype effect) and sig decreased in 5 copy plants. Not a major effect here I would say
Seeds per spike
##
## Dunnett's test for comparing several treatments with a control :
## 95% family-wise confidence level
##
## $`0`
## diff lwr.ci upr.ci pval
## 1-0 -3.306383 -15.458764 8.8459981 0.8970
## 2-0 -11.338462 -23.327959 0.6510358 0.0687 .
## 2_cadenza-0 -22.200000 -37.326911 -7.0730895 0.0017 **
## 3-0 -11.809375 -23.805561 0.1868111 0.0554 .
## 4-0 -1.958065 -13.968263 10.0521342 0.9905
## 5-0 -18.842857 -31.385429 -6.3002855 0.0013 **
## 6-0 9.800000 -3.659267 23.2592673 0.2091
## 7-0 -12.325000 -25.561047 0.9110467 0.0745 .
##
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1Note: I would conclude that there is no major effect on seeds per spike by GRF
Seed weight (total)
##
## Dunnett's test for comparing several treatments with a control :
## 95% family-wise confidence level
##
## $`0`
## diff lwr.ci upr.ci pval
## 1-0 -0.55612766 -1.2054259 0.09317060 0.11204
## 2-0 -0.79292308 -1.4335185 -0.15232765 0.01017 *
## 2_cadenza-0 -1.20257143 -2.0107979 -0.39434491 0.00145 **
## 3-0 -0.82196875 -1.4629216 -0.18101594 0.00737 **
## 4-0 -0.32077419 -0.9624757 0.32092730 0.50677
## 5-0 -1.09471429 -1.7648603 -0.42456826 0.00037 ***
## 6-0 -0.01257143 -0.7316962 0.70655339 1.00000
## 7-0 -0.75337500 -1.4605732 -0.04617679 0.03342 *
##
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1Note: Possibly a slight negative effect
Average grain width
##
## Dunnett's test for comparing several treatments with a control :
## 95% family-wise confidence level
##
## $`0`
## diff lwr.ci upr.ci pval
## 1-0 -0.238108336 -0.6247054 0.1484887 0.3341
## 2-0 -0.066720308 -0.4481356 0.3146950 0.9860
## 2_cadenza-0 0.523544600 0.0423205 1.0047687 0.0288 *
## 3-0 0.003562006 -0.3780661 0.3851901 1.0000
## 4-0 -0.038644965 -0.4207188 0.3434289 0.9995
## 5-0 0.180623886 -0.2183861 0.5796338 0.5875
## 6-0 -0.235116400 -0.6632887 0.1930559 0.4282
## 7-0 0.137722350 -0.2833487 0.5587934 0.8115
##
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1Note: No effect
Average grain length
##
## Dunnett's test for comparing several treatments with a control :
## 95% family-wise confidence level
##
## $`0`
## diff lwr.ci upr.ci pval
## 1-0 0.002421953 -0.59352779 0.5983717 1.0000
## 2-0 0.557995846 -0.02996612 1.1459578 0.0674 .
## 2_cadenza-0 1.187897686 0.44607776 1.9297176 0.0005 ***
## 3-0 0.606045463 0.01775549 1.1943354 0.0420 *
## 4-0 0.113479110 -0.47549804 0.7024563 0.9769
## 5-0 0.836359650 0.22127506 1.4514442 0.0044 **
## 6-0 -0.170526886 -0.83056600 0.4895122 0.9151
## 7-0 0.612197525 -0.03689491 1.2612900 0.0697 .
##
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1Note: Slight negative effect (?)
Infertile basal spikelets
Note: This has not been recorded for 0-copy plants so I have no control. However, I dont think there is a difference between the high and low copy number lines