Inference for numerical data

North Carolina births

In 2004, the state of North Carolina released a large data set containing information on births recorded in this state. This data set is useful to researchers studying the relation between habits and practices of expectant mothers and the birth of their children. We will work with a random sample of observations from this data set.

Exploratory analysis

Load the nc data set into our workspace.

load("more/nc.RData")

We have observations on 13 different variables, some categorical and some numerical. The meaning of each variable is as follows.

variable	description
`fage`	father’s age in years.
`mage`	mother’s age in years.
`mature`	maturity status of mother.
`weeks`	length of pregnancy in weeks.
`premie`	whether the birth was classified as premature (premie) or full-term.
`visits`	number of hospital visits during pregnancy.
`marital`	whether mother is `married` or `not married` at birth.
`gained`	weight gained by mother during pregnancy in pounds.
`weight`	weight of the baby at birth in pounds.
`lowbirthweight`	whether baby was classified as low birthweight (`low`) or not (`not low`).
`gender`	gender of the baby, `female` or `male`.
`habit`	status of the mother as a `nonsmoker` or a `smoker`.
`whitemom`	whether mom is `white` or `not white`.

What are the cases in this data set? Births in NC. How many cases are there in our sample? 1000

dim(nc)

## [1] 1000   13

As a first step in the analysis, we should consider summaries of the data. This can be done using the summary command:

summary(nc)

##       fage            mage            mature        weeks             premie   
##  Min.   :14.00   Min.   :13   mature mom :133   Min.   :20.00   full term:846  
##  1st Qu.:25.00   1st Qu.:22   younger mom:867   1st Qu.:37.00   premie   :152  
##  Median :30.00   Median :27                     Median :39.00   NA's     :  2  
##  Mean   :30.26   Mean   :27                     Mean   :38.33                  
##  3rd Qu.:35.00   3rd Qu.:32                     3rd Qu.:40.00                  
##  Max.   :55.00   Max.   :50                     Max.   :45.00                  
##  NA's   :171                                    NA's   :2                      
##      visits            marital        gained          weight      
##  Min.   : 0.0   married    :386   Min.   : 0.00   Min.   : 1.000  
##  1st Qu.:10.0   not married:613   1st Qu.:20.00   1st Qu.: 6.380  
##  Median :12.0   NA's       :  1   Median :30.00   Median : 7.310  
##  Mean   :12.1                     Mean   :30.33   Mean   : 7.101  
##  3rd Qu.:15.0                     3rd Qu.:38.00   3rd Qu.: 8.060  
##  Max.   :30.0                     Max.   :85.00   Max.   :11.750  
##  NA's   :9                        NA's   :27                      
##  lowbirthweight    gender          habit          whitemom  
##  low    :111    female:503   nonsmoker:873   not white:284  
##  not low:889    male  :497   smoker   :126   white    :714  
##                              NA's     :  1   NA's     :  2  
##                                                             
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##

As you review the variable summaries, consider which variables are categorical and which are numerical. For numerical variables, are there outliers? Yes. If you aren’t sure or want to take a closer look at the data, make a graph.

Consider the possible relationship between a mother’s smoking habit and the weight of her baby. Plotting the data is a useful first step because it helps us quickly visualize trends, identify strong associations, and develop research questions.

Make a side-by-side boxplot of habit and weight. What does the plot highlight about the relationship between these two variables? The average birthweight for babies of non-smokers is higher than that of smokers.

library(ggplot2)
p <- ggplot(nc, aes(x=habit, y=weight)) + 
  geom_boxplot()
p

The box plots show how the medians of the two distributions compare, but we can also compare the means of the distributions using the following function to split the weight variable into the habit groups, then take the mean of each using the mean function.

by(nc$weight, nc$habit, mean)

## nc$habit: nonsmoker
## [1] 7.144273
## ------------------------------------------------------------ 
## nc$habit: smoker
## [1] 6.82873

There is an observed difference, but is this difference statistically significant? In order to answer this question we will conduct a hypothesis test

Inference

Check if the conditions necessary for inference are satisfied. Note that you will need to obtain sample sizes to check the conditions. You can compute the group size using the same by command above but replacing mean with length.

by(nc$weight, nc$habit, length)

## nc$habit: nonsmoker
## [1] 873
## ------------------------------------------------------------ 
## nc$habit: smoker
## [1] 126

The conditions for inference are the…

data is normally distributed and the sample size is larger than 30

data was randomly obtained

individual observations are independent

Write the hypotheses for testing if the average weights of babies born to smoking and non-smoking mothers are different. H0: babies_weight_nonsmokers = babies_weight_smokers H1: babies_weight_nonsmokers != babies_weight_smokers

Next, we introduce a new function, inference, that we will use for conducting hypothesis tests and constructing confidence intervals.

inference(y = nc$weight, x = nc$habit, est = "mean", type = "ht", null = 0, 
          alternative = "twosided", method = "theoretical")

## Response variable: numerical, Explanatory variable: categorical
## Difference between two means
## Summary statistics:
## n_nonsmoker = 873, mean_nonsmoker = 7.1443, sd_nonsmoker = 1.5187
## n_smoker = 126, mean_smoker = 6.8287, sd_smoker = 1.3862

## Observed difference between means (nonsmoker-smoker) = 0.3155
## 
## H0: mu_nonsmoker - mu_smoker = 0 
## HA: mu_nonsmoker - mu_smoker != 0 
## Standard error = 0.134 
## Test statistic: Z =  2.359 
## p-value =  0.0184

Let’s pause for a moment to go through the arguments of this custom function. The first argument is y, which is the response variable that we are interested in: nc$weight. The second argument is the explanatory variable, x, which is the variable that splits the data into two groups, smokers and non-smokers: nc$habit. The third argument, est, is the parameter we’re interested in: "mean" (other options are "median", or "proportion".) Next we decide on the type of inference we want: a hypothesis test ("ht") or a confidence interval ("ci"). When performing a hypothesis test, we also need to supply the null value, which in this case is 0, since the null hypothesis sets the two population means equal to each other. The alternative hypothesis can be "less", "greater", or "twosided". Lastly, the method of inference can be "theoretical" or "simulation" based.

Change the type argument to "ci" to construct and record a confidence interval for the difference between the weights of babies born to smoking and non-smoking mothers.

inference(y = nc$weight, x = nc$habit, est = "mean", type = "ci", null = 0, 
          alternative = "twosided", method = "theoretical")

## Response variable: numerical, Explanatory variable: categorical
## Difference between two means
## Summary statistics:
## n_nonsmoker = 873, mean_nonsmoker = 7.1443, sd_nonsmoker = 1.5187
## n_smoker = 126, mean_smoker = 6.8287, sd_smoker = 1.3862

## Observed difference between means (nonsmoker-smoker) = 0.3155
## 
## Standard error = 0.1338 
## 95 % Confidence interval = ( 0.0534 , 0.5777 )

By default the function reports an interval for ($\mu_{nonsmoker} - \mu_{smoker}$) . We can easily change this order by using the order argument:

inference(y = nc$weight, x = nc$habit, est = "mean", type = "ci", null = 0, 
          alternative = "twosided", method = "theoretical", 
          order = c("smoker","nonsmoker"))

## Response variable: numerical, Explanatory variable: categorical
## Difference between two means
## Summary statistics:
## n_smoker = 126, mean_smoker = 6.8287, sd_smoker = 1.3862
## n_nonsmoker = 873, mean_nonsmoker = 7.1443, sd_nonsmoker = 1.5187

## Observed difference between means (smoker-nonsmoker) = -0.3155
## 
## Standard error = 0.1338 
## 95 % Confidence interval = ( -0.5777 , -0.0534 )

On your own

Calculate a 95% confidence interval for the average length of pregnancies (weeks) and interpret it in context. Note that since you’re doing inference on a single population parameter, there is no explanatory variable, so you can omit the x variable from the function.

inference(y = nc$weeks, est = "mean", type = "ci", null = 0, 
          alternative = "twosided", method = "theoretical", conflevel = 0.95)

## Single mean 
## Summary statistics:

## mean = 38.3347 ;  sd = 2.9316 ;  n = 998 
## Standard error = 0.0928 
## 95 % Confidence interval = ( 38.1528 , 38.5165 )

We can be 95% confident that the true population mean for average pregnancy length lies between 38.2 and 38.5 weeks. This is based on a sample size of 998 births and the sample was N(38.3, 2.93)

Calculate a new confidence interval for the same parameter at the 90% confidence level. You can change the confidence level by adding a new argument to the function: `conflevel = 0.90.

inference(y = nc$weeks, est = "mean", type = "ci", null = 0, 
          alternative = "twosided", method = "theoretical", conflevel = 0.90)

## Single mean 
## Summary statistics:

## mean = 38.3347 ;  sd = 2.9316 ;  n = 998 
## Standard error = 0.0928 
## 90 % Confidence interval = ( 38.182 , 38.4873 )

Conduct a hypothesis test evaluating whether the average weight gained by younger mothers is different than the average weight gained by mature mothers.

inference(y = nc$weight, x = nc$mature, est = "mean", type = "ht", null = 0, 
          alternative = "twosided", method = "theoretical", conflevel = 0.95)

## Response variable: numerical, Explanatory variable: categorical
## Difference between two means
## Summary statistics:
## n_mature mom = 133, mean_mature mom = 7.1256, sd_mature mom = 1.6591
## n_younger mom = 867, mean_younger mom = 7.0972, sd_younger mom = 1.4855

## Observed difference between means (mature mom-younger mom) = 0.0283
## 
## H0: mu_mature mom - mu_younger mom = 0 
## HA: mu_mature mom - mu_younger mom != 0 
## Standard error = 0.152 
## Test statistic: Z =  0.186 
## p-value =  0.8526

Now, a non-inference task: Determine the age cutoff for younger and mature mothers. Use a method of your choice, and explain how your method works.

p <- ggplot(nc, aes(x=mature, y=mage)) + 
  geom_boxplot()
p

The age cutoff for mature vs younger moms is 35. This is based on the above plot in which the lowest mature age is 35 and no younger Mom exceeds 35.

Pick a pair of numerical and categorical variables and come up with a research question evaluating the relationship between these variables. Formulate the question in a way that it can be answered using a hypothesis test and/or a confidence interval. Answer your question using the inference function, report the statistical results, and also provide an explanation in plain language.

Question: Is the number of hospital visits ($visits) during pregnancy significantly different for smokers versus non-smokers?

H0: smokers_hosp_visits = nonsmokers_hosp_visits H1: smokers_hosp_visits != nonsmokers_hosp_visits

inference(y = nc$visits, x = nc$habit, est = "mean", type = "ht", null = 0, 
          alternative = "twosided", method = "theoretical", conflevel = 0.95)

## Response variable: numerical, Explanatory variable: categorical
## Difference between two means
## Summary statistics:
## n_nonsmoker = 866, mean_nonsmoker = 12.2159, sd_nonsmoker = 3.9139
## n_smoker = 125, mean_smoker = 11.336, sd_smoker = 4.1639

## Observed difference between means (nonsmoker-smoker) = 0.8799
## 
## H0: mu_nonsmoker - mu_smoker = 0 
## HA: mu_nonsmoker - mu_smoker != 0 
## Standard error = 0.395 
## Test statistic: Z =  2.225 
## p-value =  0.026

The statistical results are… n_nonsmoker = 866, mean_nonsmoker = 12.2159, sd_nonsmoker = 3.9139 n_smoker = 125, mean_smoker = 11.336, sd_smoker = 4.1639

Observed difference between means (nonsmoker-smoker) = 0.8799

Standard error = 0.395 Test statistic: Z = 2.225 p-value = 0.026

Non-smokers average 12 hospital visits vs smokers 11 visits over the course of their pregnancy. Based on the p-value of 0.03 < 0.05, we can reject the null hypothesis; based on this study non-smokers have more hospital visits than smokers over the course of their pregnancy.