Fragment size distribution of merged overlapping reads vs. aligned reads

Species: E.coli K-12 MG1655
Data: https://basespace.illumina.com/sample/3756762/Ecoli
Merged overlapping reads using abyss-mergepairs -q 15
Aligned reads to an assembly of the same reads using ABySS 1.5.2

library(dplyr)
library(ggplot2)
library(knitr)
library(tidyr)

merged.data <- read.table("ecoli_merged.fastq.hist",
    col.names = c("Fragment.size", "Count"))
aligned.data <- read.table("k364/pe600-3.hist",
    col.names = c("Fragment.size", "Count"))
data <- rbind(
    merged.data %>% cbind(data.frame(Group = "Merged")),
    aligned.data %>% cbind(data.frame(Group = "Aligned")))

aligned.stats <- with(aligned.data, rep.int(x = Fragment.size, times = Count)) %>%
    boxplot.stats(coef = 3)
str(aligned.stats)

## List of 4
##  $ stats: num [1:5] 41 497 580 676 1213
##  $ n    : int 2129426
##  $ conf : num [1:2] 580 580
##  $ out  : int [1:313] -72474 -44225 -39143 -27500 -18590 -16724 -10959 -10950 -10949 -10942 ...

ggplot(data = data) +
    aes(x = Fragment.size, y = Count, group = Group, colour = Group) +
    geom_point(size = 1) + geom_smooth() +
    xlim(aligned.stats$stats[1], aligned.stats$stats[5])

## Warning: Removed 3 rows containing missing values (stat_smooth).

## Warning: Removed 210 rows containing missing values (stat_smooth).

## Warning: Removed 213 rows containing missing values (geom_point).

kable(data)

Fragment.size	Count	Group
36	2	Merged
37	1	Merged
39	1	Merged
41	1	Merged
42	1	Merged
46	1	Merged
47	2	Merged
48	1	Merged
49	1	Merged
51	3	Merged
54	2	Merged
55	1	Merged
56	3	Merged
57	2	Merged
59	1	Merged
60	1	Merged
61	1	Merged
62	3	Merged
63	3	Merged
64	6	Merged
65	4	Merged
66	2	Merged
67	3	Merged
68	3	Merged
69	7	Merged
70	9	Merged
71	3	Merged
72	5	Merged
73	2	Merged
74	7	Merged
75	4	Merged
76	4	Merged
77	4	Merged
78	4	Merged
79	9	Merged
80	9	Merged
81	7	Merged
82	6	Merged
83	10	Merged
84	7	Merged
85	11	Merged
86	8	Merged
87	7	Merged
88	8	Merged
89	7	Merged
90	7	Merged
91	11	Merged
92	10	Merged
93	7	Merged
94	4	Merged
95	13	Merged
96	13	Merged
97	14	Merged
98	15	Merged
99	12	Merged
100	14	Merged
101	11	Merged
102	18	Merged
103	14	Merged
104	21	Merged
105	18	Merged
106	25	Merged
107	11	Merged
108	28	Merged
109	23	Merged
110	20	Merged
111	25	Merged
112	22	Merged
113	19	Merged
114	33	Merged
115	34	Merged
116	29	Merged
117	30	Merged
118	36	Merged
119	32	Merged
120	46	Merged
121	37	Merged
122	50	Merged
123	55	Merged
124	56	Merged
125	48	Merged
126	42	Merged
127	61	Merged
128	67	Merged
129	65	Merged
130	59	Merged
131	73	Merged
132	67	Merged
133	77	Merged
134	70	Merged
135	75	Merged
136	64	Merged
137	72	Merged
138	92	Merged
139	71	Merged
140	87	Merged
141	89	Merged
142	87	Merged
143	100	Merged
144	121	Merged
145	102	Merged
146	113	Merged
147	100	Merged
148	101	Merged
149	107	Merged
150	99	Merged
151	116	Merged
152	103	Merged
153	103	Merged
154	125	Merged
155	114	Merged
156	130	Merged
157	114	Merged
158	124	Merged
159	124	Merged
160	135	Merged
161	124	Merged
162	126	Merged
163	116	Merged
164	154	Merged
165	140	Merged
166	133	Merged
167	117	Merged
168	143	Merged
169	139	Merged
170	144	Merged
171	143	Merged
172	157	Merged
173	138	Merged
174	139	Merged
175	153	Merged
176	133	Merged
177	166	Merged
178	147	Merged
179	166	Merged
180	141	Merged
181	159	Merged
182	157	Merged
183	179	Merged
184	166	Merged
185	158	Merged
186	159	Merged
187	159	Merged
188	163	Merged
189	165	Merged
190	181	Merged
191	197	Merged
192	191	Merged
193	163	Merged
194	201	Merged
195	176	Merged
196	205	Merged
197	169	Merged
198	207	Merged
199	222	Merged
200	189	Merged
201	175	Merged
202	203	Merged
203	189	Merged
204	204	Merged
205	189	Merged
206	194	Merged
207	191	Merged
208	236	Merged
209	206	Merged
210	213	Merged
211	183	Merged
212	200	Merged
213	213	Merged
214	202	Merged
215	219	Merged
216	192	Merged
217	197	Merged
218	201	Merged
219	223	Merged
220	212	Merged
221	239	Merged
222	207	Merged
223	233	Merged
224	205	Merged
225	231	Merged
226	254	Merged
227	224	Merged
228	273	Merged
229	243	Merged
230	252	Merged
231	275	Merged
232	274	Merged
233	250	Merged
234	249	Merged
235	222	Merged
236	276	Merged
237	272	Merged
238	286	Merged
239	273	Merged
240	298	Merged
241	288	Merged
242	296	Merged
243	262	Merged
244	309	Merged
245	301	Merged
246	298	Merged
247	272	Merged
248	280	Merged
249	302	Merged
250	276	Merged
251	341	Merged
252	332	Merged
253	310	Merged
254	325	Merged
255	309	Merged
256	324	Merged
257	322	Merged
258	307	Merged
259	332	Merged
260	340	Merged
261	344	Merged
262	345	Merged
263	380	Merged
264	362	Merged
265	368	Merged
266	372	Merged
267	365	Merged
268	375	Merged
269	382	Merged
270	350	Merged
271	379	Merged
272	376	Merged
273	375	Merged
274	382	Merged
275	409	Merged
276	329	Merged
277	417	Merged
278	414	Merged
279	384	Merged
280	408	Merged
281	434	Merged
282	372	Merged
283	316	Merged
284	462	Merged
285	445	Merged
286	407	Merged
287	459	Merged
288	395	Merged
289	457	Merged
290	465	Merged
291	504	Merged
292	454	Merged
293	300	Merged
294	471	Merged
295	428	Merged
296	482	Merged
297	247	Merged
298	541	Merged
299	445	Merged
300	504	Merged
301	610	Merged
302	624	Merged
303	635	Merged
304	603	Merged
305	664	Merged
306	659	Merged
307	644	Merged
308	655	Merged
309	664	Merged
310	671	Merged
311	690	Merged
312	679	Merged
313	670	Merged
314	722	Merged
315	710	Merged
316	686	Merged
317	757	Merged
318	739	Merged
319	750	Merged
320	759	Merged
321	783	Merged
322	796	Merged
323	784	Merged
324	793	Merged
325	811	Merged
326	782	Merged
327	782	Merged
328	840	Merged
329	934	Merged
330	844	Merged
331	855	Merged
332	863	Merged
333	907	Merged
334	899	Merged
335	871	Merged
336	955	Merged
337	954	Merged
338	927	Merged
339	910	Merged
340	961	Merged
341	1000	Merged
342	1059	Merged
343	1010	Merged
344	1062	Merged
345	1060	Merged
346	1055	Merged
347	1049	Merged
348	1122	Merged
349	1124	Merged
350	1136	Merged
351	1164	Merged
352	1098	Merged
353	1182	Merged
354	1151	Merged
355	1220	Merged
356	1270	Merged
357	1273	Merged
358	1243	Merged
359	1271	Merged
360	1274	Merged
361	1328	Merged
362	1304	Merged
363	1375	Merged
364	1320	Merged
365	1377	Merged
366	1427	Merged
367	1405	Merged
368	1418	Merged
369	1378	Merged
370	1550	Merged
371	1495	Merged
372	1467	Merged
373	1497	Merged
374	1591	Merged
375	1538	Merged
376	1600	Merged
377	1637	Merged
378	1684	Merged
379	1635	Merged
380	1669	Merged
381	1675	Merged
382	1679	Merged
383	1677	Merged
384	1752	Merged
385	1746	Merged
386	1839	Merged
387	1813	Merged
388	1888	Merged
389	1796	Merged
390	1878	Merged
391	1837	Merged
392	1979	Merged
393	1929	Merged
394	1928	Merged
395	2030	Merged
396	2069	Merged
397	2056	Merged
398	2143	Merged
399	2075	Merged
400	2089	Merged
401	2126	Merged
402	2236	Merged
403	2169	Merged
404	2202	Merged
405	2329	Merged
406	2244	Merged
407	2350	Merged
408	2410	Merged
409	2430	Merged
410	2405	Merged
411	2439	Merged
412	2444	Merged
413	2577	Merged
414	2501	Merged
415	2632	Merged
416	2569	Merged
417	2494	Merged
418	2593	Merged
419	2749	Merged
420	2696	Merged
421	2760	Merged
422	2813	Merged
423	2898	Merged
424	2790	Merged
425	2815	Merged
426	2897	Merged
427	2969	Merged
428	2967	Merged
429	3013	Merged
430	3061	Merged
431	3108	Merged
432	3207	Merged
433	3198	Merged
434	3146	Merged
435	3229	Merged
436	3200	Merged
437	3314	Merged
438	3300	Merged
439	3450	Merged
440	3475	Merged
441	3481	Merged
442	3354	Merged
443	3497	Merged
444	3546	Merged
445	3571	Merged
446	3563	Merged
447	3661	Merged
448	3720	Merged
449	3747	Merged
450	3615	Merged
451	3828	Merged
452	3767	Merged
453	3780	Merged
454	3901	Merged
455	3899	Merged
456	3936	Merged
457	4028	Merged
458	4135	Merged
459	4139	Merged
460	4078	Merged
461	4112	Merged
462	4151	Merged
463	4210	Merged
464	4344	Merged
465	4283	Merged
466	4326	Merged
467	4435	Merged
468	4488	Merged
469	4458	Merged
470	4429	Merged
471	4475	Merged
472	4454	Merged
473	4534	Merged
474	4678	Merged
475	4650	Merged
476	4717	Merged
477	4852	Merged
478	4776	Merged
479	4804	Merged
480	4727	Merged
481	4765	Merged
482	4978	Merged
483	4951	Merged
484	4939	Merged
485	4970	Merged
486	4816	Merged
487	5157	Merged
488	5030	Merged
489	5130	Merged
490	5075	Merged
491	5329	Merged
492	5262	Merged
493	5233	Merged
494	5294	Merged
495	5323	Merged
496	5421	Merged
497	5329	Merged
498	5450	Merged
499	5440	Merged
500	5381	Merged
501	5434	Merged
502	5520	Merged
503	5595	Merged
504	5485	Merged
505	5640	Merged
506	5533	Merged
507	5541	Merged
508	5672	Merged
509	5676	Merged
510	5612	Merged
511	5553	Merged
512	5720	Merged
513	5787	Merged
514	5809	Merged
515	5830	Merged
516	5954	Merged
517	5963	Merged
518	5819	Merged
519	5796	Merged
520	5936	Merged
521	5969	Merged
522	5851	Merged
523	5993	Merged
524	5899	Merged
525	5954	Merged
526	5931	Merged
527	5994	Merged
528	6112	Merged
529	6036	Merged
530	6030	Merged
531	6099	Merged
532	6027	Merged
533	6108	Merged
534	6064	Merged
535	6130	Merged
536	6178	Merged
537	6083	Merged
538	6133	Merged
539	6170	Merged
540	6020	Merged
541	6031	Merged
542	6017	Merged
543	5974	Merged
544	6044	Merged
545	6029	Merged
546	6145	Merged
547	5962	Merged
548	6054	Merged
549	5996	Merged
550	6133	Merged
551	6040	Merged
552	5948	Merged
553	5956	Merged
554	5938	Merged
555	5916	Merged
556	5968	Merged
557	5973	Merged
558	5958	Merged
559	5893	Merged
560	5973	Merged
561	5859	Merged
562	5909	Merged
563	5853	Merged
564	5717	Merged
565	5779	Merged
566	5942	Merged
567	5900	Merged
568	5813	Merged
569	5693	Merged
570	5781	Merged
571	5641	Merged
572	5558	Merged
573	5448	Merged
574	5451	Merged
575	5549	Merged
576	5648	Merged
577	5580	Merged
578	5576	Merged
579	5580	Merged
580	5440	Merged
581	5361	Merged
582	5197	Merged
583	5073	Merged
584	5237	Merged
585	5323	Merged
586	5328	Merged
587	5286	Merged
588	5117	Merged
589	4863	Merged
590	4228	Merged
591	2885	Merged
592	959	Merged
-72474	1	Aligned
-44225	1	Aligned
-39143	1	Aligned
-27500	1	Aligned
-18590	1	Aligned
-16724	1	Aligned
-10959	1	Aligned
-10950	1	Aligned
-10949	1	Aligned
-10942	1	Aligned
-10938	1	Aligned
-10929	1	Aligned
-10928	1	Aligned
-10926	1	Aligned
-10924	1	Aligned
-10919	1	Aligned
-10916	1	Aligned
-10904	1	Aligned
-10901	1	Aligned
-10895	1	Aligned
-10894	1	Aligned
-10887	1	Aligned
-10886	1	Aligned
-10884	2	Aligned
-10876	1	Aligned
-10868	1	Aligned
-10867	1	Aligned
-10866	1	Aligned
-10865	1	Aligned
-10864	1	Aligned
-10859	2	Aligned
-10851	1	Aligned
-10849	1	Aligned
-10846	2	Aligned
-10845	1	Aligned
-10843	2	Aligned
-10840	1	Aligned
-10836	2	Aligned
-10834	1	Aligned
-10831	1	Aligned
-10827	3	Aligned
-10823	2	Aligned
-10822	1	Aligned
-10820	1	Aligned
-10817	1	Aligned
-10815	1	Aligned
-10812	1	Aligned
-10804	1	Aligned
-10800	1	Aligned
-10797	1	Aligned
-10791	1	Aligned
-10788	1	Aligned
-10786	1	Aligned
-10785	1	Aligned
-10784	1	Aligned
-10777	2	Aligned
-10772	1	Aligned
-10766	1	Aligned
-10765	1	Aligned
-10762	1	Aligned
-10755	1	Aligned
-10752	2	Aligned
-10750	1	Aligned
-10745	1	Aligned
-10737	1	Aligned
-10734	1	Aligned
-10729	1	Aligned
-10724	1	Aligned
-10722	1	Aligned
-10720	1	Aligned
-10717	1	Aligned
-10716	1	Aligned
-10714	1	Aligned
-10713	1	Aligned
-10700	1	Aligned
-10696	1	Aligned
-10681	1	Aligned
-10671	1	Aligned
-10652	1	Aligned
-10640	1	Aligned
-10632	1	Aligned
-10598	1	Aligned
-10594	1	Aligned
-10572	1	Aligned
-10564	1	Aligned
-10505	1	Aligned
-1386	1	Aligned
-1364	1	Aligned
-1323	1	Aligned
-1254	1	Aligned
-1253	1	Aligned
-1225	1	Aligned
-1217	1	Aligned
-1194	1	Aligned
-1079	1	Aligned
-1061	1	Aligned
-843	1	Aligned
-467	1	Aligned
-450	1	Aligned
-437	1	Aligned
-420	1	Aligned
-382	1	Aligned
-370	1	Aligned
-355	1	Aligned
-293	1	Aligned
-83	1	Aligned
-69	1	Aligned
-49	1	Aligned
41	1	Aligned
46	3	Aligned
47	2	Aligned
48	3	Aligned
49	1	Aligned
51	2	Aligned
54	2	Aligned
56	3	Aligned
57	2	Aligned
58	2	Aligned
59	1	Aligned
60	2	Aligned
61	2	Aligned
62	4	Aligned
63	5	Aligned
64	7	Aligned
65	4	Aligned
66	3	Aligned
67	3	Aligned
68	3	Aligned
69	8	Aligned
70	11	Aligned
71	3	Aligned
72	5	Aligned
73	2	Aligned
74	7	Aligned
75	4	Aligned
76	4	Aligned
77	4	Aligned
78	5	Aligned
79	9	Aligned
80	10	Aligned
81	8	Aligned
82	6	Aligned
83	10	Aligned
84	9	Aligned
85	13	Aligned
86	9	Aligned
87	6	Aligned
88	8	Aligned
89	6	Aligned
90	8	Aligned
91	11	Aligned
92	10	Aligned
93	7	Aligned
94	7	Aligned
95	15	Aligned
96	14	Aligned
97	17	Aligned
98	15	Aligned
99	12	Aligned
100	15	Aligned
101	12	Aligned
102	20	Aligned
103	17	Aligned
104	24	Aligned
105	19	Aligned
106	27	Aligned
107	13	Aligned
108	28	Aligned
109	24	Aligned
110	19	Aligned
111	26	Aligned
112	24	Aligned
113	21	Aligned
114	34	Aligned
115	36	Aligned
116	33	Aligned
117	33	Aligned
118	39	Aligned
119	35	Aligned
120	49	Aligned
121	41	Aligned
122	60	Aligned
123	57	Aligned
124	62	Aligned
125	49	Aligned
126	45	Aligned
127	65	Aligned
128	71	Aligned
129	67	Aligned
130	65	Aligned
131	73	Aligned
132	72	Aligned
133	80	Aligned
134	74	Aligned
135	77	Aligned
136	67	Aligned
137	77	Aligned
138	97	Aligned
139	75	Aligned
140	99	Aligned
141	96	Aligned
142	91	Aligned
143	110	Aligned
144	127	Aligned
145	110	Aligned
146	113	Aligned
147	108	Aligned
148	104	Aligned
149	111	Aligned
150	108	Aligned
151	125	Aligned
152	112	Aligned
153	111	Aligned
154	126	Aligned
155	120	Aligned
156	131	Aligned
157	127	Aligned
158	133	Aligned
159	129	Aligned
160	143	Aligned
161	129	Aligned
162	133	Aligned
163	122	Aligned
164	161	Aligned
165	148	Aligned
166	145	Aligned
167	118	Aligned
168	152	Aligned
169	152	Aligned
170	152	Aligned
171	152	Aligned
172	167	Aligned
173	148	Aligned
174	149	Aligned
175	165	Aligned
176	140	Aligned
177	171	Aligned
178	157	Aligned
179	176	Aligned
180	144	Aligned
181	166	Aligned
182	166	Aligned
183	187	Aligned
184	171	Aligned
185	173	Aligned
186	169	Aligned
187	170	Aligned
188	172	Aligned
189	176	Aligned
190	196	Aligned
191	209	Aligned
192	198	Aligned
193	176	Aligned
194	209	Aligned
195	187	Aligned
196	214	Aligned
197	178	Aligned
198	215	Aligned
199	232	Aligned
200	193	Aligned
201	186	Aligned
202	206	Aligned
203	205	Aligned
204	217	Aligned
205	202	Aligned
206	206	Aligned
207	201	Aligned
208	251	Aligned
209	214	Aligned
210	231	Aligned
211	195	Aligned
212	209	Aligned
213	225	Aligned
214	223	Aligned
215	228	Aligned
216	204	Aligned
217	208	Aligned
218	207	Aligned
219	236	Aligned
220	222	Aligned
221	255	Aligned
222	217	Aligned
223	250	Aligned
224	230	Aligned
225	243	Aligned
226	271	Aligned
227	244	Aligned
228	290	Aligned
229	255	Aligned
230	271	Aligned
231	294	Aligned
232	294	Aligned
233	272	Aligned
234	256	Aligned
235	246	Aligned
236	297	Aligned
237	286	Aligned
238	312	Aligned
239	282	Aligned
240	314	Aligned
241	318	Aligned
242	322	Aligned
243	287	Aligned
244	331	Aligned
245	319	Aligned
246	316	Aligned
247	296	Aligned
248	297	Aligned
249	325	Aligned
250	308	Aligned
251	366	Aligned
252	354	Aligned
253	327	Aligned
254	356	Aligned
255	345	Aligned
256	361	Aligned
257	358	Aligned
258	334	Aligned
259	361	Aligned
260	378	Aligned
261	381	Aligned
262	372	Aligned
263	416	Aligned
264	409	Aligned
265	410	Aligned
266	401	Aligned
267	416	Aligned
268	430	Aligned
269	430	Aligned
270	403	Aligned
271	413	Aligned
272	444	Aligned
273	422	Aligned
274	425	Aligned
275	471	Aligned
276	407	Aligned
277	472	Aligned
278	478	Aligned
279	444	Aligned
280	463	Aligned
281	495	Aligned
282	462	Aligned
283	457	Aligned
284	525	Aligned
285	513	Aligned
286	490	Aligned
287	513	Aligned
288	449	Aligned
289	496	Aligned
290	507	Aligned
291	546	Aligned
292	559	Aligned
293	521	Aligned
294	555	Aligned
295	533	Aligned
296	590	Aligned
297	577	Aligned
298	614	Aligned
299	549	Aligned
300	535	Aligned
301	612	Aligned
302	625	Aligned
303	631	Aligned
304	605	Aligned
305	658	Aligned
306	659	Aligned
307	642	Aligned
308	658	Aligned
309	669	Aligned
310	662	Aligned
311	692	Aligned
312	674	Aligned
313	670	Aligned
314	728	Aligned
315	712	Aligned
316	685	Aligned
317	752	Aligned
318	745	Aligned
319	749	Aligned
320	759	Aligned
321	774	Aligned
322	794	Aligned
323	784	Aligned
324	792	Aligned
325	805	Aligned
326	783	Aligned
327	776	Aligned
328	838	Aligned
329	922	Aligned
330	847	Aligned
331	854	Aligned
332	857	Aligned
333	908	Aligned
334	901	Aligned
335	870	Aligned
336	959	Aligned
337	954	Aligned
338	923	Aligned
339	913	Aligned
340	958	Aligned
341	1005	Aligned
342	1058	Aligned
343	1003	Aligned
344	1059	Aligned
345	1064	Aligned
346	1057	Aligned
347	1044	Aligned
348	1116	Aligned
349	1128	Aligned
350	1138	Aligned
351	1161	Aligned
352	1103	Aligned
353	1167	Aligned
354	1148	Aligned
355	1221	Aligned
356	1262	Aligned
357	1279	Aligned
358	1238	Aligned
359	1275	Aligned
360	1274	Aligned
361	1323	Aligned
362	1309	Aligned
363	1364	Aligned
364	1319	Aligned
365	1381	Aligned
366	1421	Aligned
367	1404	Aligned
368	1415	Aligned
369	1365	Aligned
370	1543	Aligned
371	1488	Aligned
372	1477	Aligned
373	1497	Aligned
374	1591	Aligned
375	1544	Aligned
376	1611	Aligned
377	1639	Aligned
378	1685	Aligned
379	1644	Aligned
380	1680	Aligned
381	1678	Aligned
382	1682	Aligned
383	1684	Aligned
384	1754	Aligned
385	1750	Aligned
386	1820	Aligned
387	1818	Aligned
388	1891	Aligned
389	1800	Aligned
390	1873	Aligned
391	1839	Aligned
392	1978	Aligned
393	1932	Aligned
394	1924	Aligned
395	2040	Aligned
396	2060	Aligned
397	2051	Aligned
398	2154	Aligned
399	2072	Aligned
400	2099	Aligned
401	2129	Aligned
402	2228	Aligned
403	2163	Aligned
404	2213	Aligned
405	2351	Aligned
406	2249	Aligned
407	2365	Aligned
408	2406	Aligned
409	2416	Aligned
410	2413	Aligned
411	2434	Aligned
412	2461	Aligned
413	2593	Aligned
414	2516	Aligned
415	2637	Aligned
416	2582	Aligned
417	2497	Aligned
418	2612	Aligned
419	2748	Aligned
420	2712	Aligned
421	2787	Aligned
422	2817	Aligned
423	2909	Aligned
424	2828	Aligned
425	2840	Aligned
426	2914	Aligned
427	2976	Aligned
428	2988	Aligned
429	3041	Aligned
430	3092	Aligned
431	3122	Aligned
432	3235	Aligned
433	3230	Aligned
434	3169	Aligned
435	3234	Aligned
436	3218	Aligned
437	3325	Aligned
438	3320	Aligned
439	3487	Aligned
440	3507	Aligned
441	3481	Aligned
442	3389	Aligned
443	3537	Aligned
444	3586	Aligned
445	3615	Aligned
446	3582	Aligned
447	3677	Aligned
448	3753	Aligned
449	3794	Aligned
450	3662	Aligned
451	3872	Aligned
452	3809	Aligned
453	3818	Aligned
454	3943	Aligned
455	3944	Aligned
456	3979	Aligned
457	4060	Aligned
458	4214	Aligned
459	4182	Aligned
460	4131	Aligned
461	4174	Aligned
462	4242	Aligned
463	4304	Aligned
464	4411	Aligned
465	4345	Aligned
466	4373	Aligned
467	4472	Aligned
468	4561	Aligned
469	4499	Aligned
470	4497	Aligned
471	4561	Aligned
472	4525	Aligned
473	4601	Aligned
474	4752	Aligned
475	4754	Aligned
476	4815	Aligned
477	4934	Aligned
478	4902	Aligned
479	4896	Aligned
480	4866	Aligned
481	4843	Aligned
482	5119	Aligned
483	5064	Aligned
484	5076	Aligned
485	5084	Aligned
486	4939	Aligned
487	5265	Aligned
488	5168	Aligned
489	5287	Aligned
490	5200	Aligned
491	5475	Aligned
492	5433	Aligned
493	5405	Aligned
494	5474	Aligned
495	5485	Aligned
496	5576	Aligned
497	5503	Aligned
498	5620	Aligned
499	5643	Aligned
500	5577	Aligned
501	5631	Aligned
502	5713	Aligned
503	5830	Aligned
504	5689	Aligned
505	5867	Aligned
506	5740	Aligned
507	5742	Aligned
508	5912	Aligned
509	5883	Aligned
510	5831	Aligned
511	5809	Aligned
512	5983	Aligned
513	6037	Aligned
514	6079	Aligned
515	6051	Aligned
516	6255	Aligned
517	6225	Aligned
518	6103	Aligned
519	6126	Aligned
520	6182	Aligned
521	6277	Aligned
522	6157	Aligned
523	6320	Aligned
524	6300	Aligned
525	6299	Aligned
526	6258	Aligned
527	6364	Aligned
528	6439	Aligned
529	6404	Aligned
530	6413	Aligned
531	6455	Aligned
532	6481	Aligned
533	6566	Aligned
534	6558	Aligned
535	6588	Aligned
536	6612	Aligned
537	6576	Aligned
538	6591	Aligned
539	6598	Aligned
540	6539	Aligned
541	6580	Aligned
542	6581	Aligned
543	6540	Aligned
544	6631	Aligned
545	6618	Aligned
546	6731	Aligned
547	6549	Aligned
548	6638	Aligned
549	6619	Aligned
550	6734	Aligned
551	6736	Aligned
552	6585	Aligned
553	6661	Aligned
554	6639	Aligned
555	6653	Aligned
556	6687	Aligned
557	6648	Aligned
558	6684	Aligned
559	6613	Aligned
560	6708	Aligned
561	6625	Aligned
562	6760	Aligned
563	6715	Aligned
564	6594	Aligned
565	6652	Aligned
566	6788	Aligned
567	6760	Aligned
568	6653	Aligned
569	6557	Aligned
570	6659	Aligned
571	6585	Aligned
572	6652	Aligned
573	6566	Aligned
574	6536	Aligned
575	6632	Aligned
576	6674	Aligned
577	6608	Aligned
578	6559	Aligned
579	6568	Aligned
580	6506	Aligned
581	6532	Aligned
582	6523	Aligned
583	6476	Aligned
584	6614	Aligned
585	6615	Aligned
586	6626	Aligned
587	6542	Aligned
588	6506	Aligned
589	6490	Aligned
590	6426	Aligned
591	6443	Aligned
592	6425	Aligned
593	6552	Aligned
594	6350	Aligned
595	6302	Aligned
596	6539	Aligned
597	6351	Aligned
598	6216	Aligned
599	6441	Aligned
600	6222	Aligned
601	6233	Aligned
602	6325	Aligned
603	6204	Aligned
604	6106	Aligned
605	6170	Aligned
606	6117	Aligned
607	6012	Aligned
608	6169	Aligned
609	5954	Aligned
610	6226	Aligned
611	6097	Aligned
612	5904	Aligned
613	5937	Aligned
614	5867	Aligned
615	6034	Aligned
616	5950	Aligned
617	5904	Aligned
618	5923	Aligned
619	5839	Aligned
620	5910	Aligned
621	5845	Aligned
622	5820	Aligned
623	5805	Aligned
624	5924	Aligned
625	5799	Aligned
626	5600	Aligned
627	5646	Aligned
628	5582	Aligned
629	5659	Aligned
630	5547	Aligned
631	5621	Aligned
632	5565	Aligned
633	5374	Aligned
634	5640	Aligned
635	5431	Aligned
636	5362	Aligned
637	5427	Aligned
638	5451	Aligned
639	5349	Aligned
640	5448	Aligned
641	5430	Aligned
642	5242	Aligned
643	5118	Aligned
644	5190	Aligned
645	5223	Aligned
646	5277	Aligned
647	5110	Aligned
648	5138	Aligned
649	5191	Aligned
650	5189	Aligned
651	5002	Aligned
652	4991	Aligned
653	4942	Aligned
654	5152	Aligned
655	4988	Aligned
656	4869	Aligned
657	4889	Aligned
658	4878	Aligned
659	4876	Aligned
660	4816	Aligned
661	4595	Aligned
662	4659	Aligned
663	4641	Aligned
664	4702	Aligned
665	4580	Aligned
666	4520	Aligned
667	4513	Aligned
668	4573	Aligned
669	4498	Aligned
670	4529	Aligned
671	4444	Aligned
672	4408	Aligned
673	4446	Aligned
674	4351	Aligned
675	4325	Aligned
676	4368	Aligned
677	4223	Aligned
678	4407	Aligned
679	4396	Aligned
680	4300	Aligned
681	4160	Aligned
682	4059	Aligned
683	4122	Aligned
684	4105	Aligned
685	4064	Aligned
686	4134	Aligned
687	4017	Aligned
688	3894	Aligned
689	4034	Aligned
690	3886	Aligned
691	3908	Aligned
692	3809	Aligned
693	3976	Aligned
694	3768	Aligned
695	3876	Aligned
696	3677	Aligned
697	3907	Aligned
698	3775	Aligned
699	3689	Aligned
700	3694	Aligned
701	3636	Aligned
702	3744	Aligned
703	3593	Aligned
704	3595	Aligned
705	3522	Aligned
706	3534	Aligned
707	3522	Aligned
708	3624	Aligned
709	3516	Aligned
710	3437	Aligned
711	3393	Aligned
712	3431	Aligned
713	3455	Aligned
714	3341	Aligned
715	3355	Aligned
716	3329	Aligned
717	3229	Aligned
718	3232	Aligned
719	3218	Aligned
720	3161	Aligned
721	3187	Aligned
722	3248	Aligned
723	3130	Aligned
724	3014	Aligned
725	3166	Aligned
726	3109	Aligned
727	3098	Aligned
728	3112	Aligned
729	3035	Aligned
730	2957	Aligned
731	3062	Aligned
732	2858	Aligned
733	2925	Aligned
734	2936	Aligned
735	2813	Aligned
736	2926	Aligned
737	2814	Aligned
738	2834	Aligned
739	2770	Aligned
740	2756	Aligned
741	2812	Aligned
742	2719	Aligned
743	2655	Aligned
744	2664	Aligned
745	2711	Aligned
746	2687	Aligned
747	2531	Aligned
748	2641	Aligned
749	2625	Aligned
750	2627	Aligned
751	2578	Aligned
752	2521	Aligned
753	2539	Aligned
754	2526	Aligned
755	2511	Aligned
756	2359	Aligned
757	2457	Aligned
758	2457	Aligned
759	2370	Aligned
760	2393	Aligned
761	2328	Aligned
762	2304	Aligned
763	2298	Aligned
764	2219	Aligned
765	2276	Aligned
766	2273	Aligned
767	2256	Aligned
768	2211	Aligned
769	2277	Aligned
770	2244	Aligned
771	2189	Aligned
772	2111	Aligned
773	2165	Aligned
774	2146	Aligned
775	2026	Aligned
776	2096	Aligned
777	2026	Aligned
778	2047	Aligned
779	2037	Aligned
780	2002	Aligned
781	2006	Aligned
782	2060	Aligned
783	1951	Aligned
784	2039	Aligned
785	1942	Aligned
786	1980	Aligned
787	1967	Aligned
788	1832	Aligned
789	1891	Aligned
790	1837	Aligned
791	1821	Aligned
792	1904	Aligned
793	1783	Aligned
794	1837	Aligned
795	1764	Aligned
796	1842	Aligned
797	1754	Aligned
798	1695	Aligned
799	1768	Aligned
800	1744	Aligned
801	1681	Aligned
802	1736	Aligned
803	1726	Aligned
804	1677	Aligned
805	1658	Aligned
806	1605	Aligned
807	1620	Aligned
808	1637	Aligned
809	1605	Aligned
810	1596	Aligned
811	1616	Aligned
812	1567	Aligned
813	1544	Aligned
814	1469	Aligned
815	1578	Aligned
816	1479	Aligned
817	1422	Aligned
818	1492	Aligned
819	1461	Aligned
820	1453	Aligned
821	1449	Aligned
822	1459	Aligned
823	1334	Aligned
824	1444	Aligned
825	1424	Aligned
826	1364	Aligned
827	1373	Aligned
828	1360	Aligned
829	1370	Aligned
830	1360	Aligned
831	1347	Aligned
832	1346	Aligned
833	1334	Aligned
834	1338	Aligned
835	1294	Aligned
836	1276	Aligned
837	1217	Aligned
838	1232	Aligned
839	1288	Aligned
840	1257	Aligned
841	1276	Aligned
842	1193	Aligned
843	1192	Aligned
844	1221	Aligned
845	1208	Aligned
846	1093	Aligned
847	1154	Aligned
848	1156	Aligned
849	1142	Aligned
850	1128	Aligned
851	1184	Aligned
852	1125	Aligned
853	1117	Aligned
854	1031	Aligned
855	1116	Aligned
856	1010	Aligned
857	1050	Aligned
858	1038	Aligned
859	1102	Aligned
860	945	Aligned
861	1014	Aligned
862	1016	Aligned
863	970	Aligned
864	997	Aligned
865	953	Aligned
866	956	Aligned
867	963	Aligned
868	977	Aligned
869	887	Aligned
870	917	Aligned
871	910	Aligned
872	893	Aligned
873	932	Aligned
874	913	Aligned
875	857	Aligned
876	877	Aligned
877	865	Aligned
878	880	Aligned
879	848	Aligned
880	801	Aligned
881	828	Aligned
882	835	Aligned
883	803	Aligned
884	809	Aligned
885	825	Aligned
886	819	Aligned
887	776	Aligned
888	813	Aligned
889	783	Aligned
890	797	Aligned
891	756	Aligned
892	719	Aligned
893	753	Aligned
894	752	Aligned
895	717	Aligned
896	798	Aligned
897	719	Aligned
898	770	Aligned
899	690	Aligned
900	724	Aligned
901	647	Aligned
902	688	Aligned
903	683	Aligned
904	692	Aligned
905	653	Aligned
906	669	Aligned
907	647	Aligned
908	661	Aligned
909	604	Aligned
910	664	Aligned
911	613	Aligned
912	616	Aligned
913	570	Aligned
914	611	Aligned
915	607	Aligned
916	594	Aligned
917	575	Aligned
918	561	Aligned
919	610	Aligned
920	557	Aligned
921	583	Aligned
922	554	Aligned
923	539	Aligned
924	530	Aligned
925	527	Aligned
926	557	Aligned
927	550	Aligned
928	493	Aligned
929	562	Aligned
930	497	Aligned
931	555	Aligned
932	485	Aligned
933	501	Aligned
934	515	Aligned
935	478	Aligned
936	495	Aligned
937	458	Aligned
938	441	Aligned
939	460	Aligned
940	462	Aligned
941	438	Aligned
942	422	Aligned
943	410	Aligned
944	425	Aligned
945	472	Aligned
946	419	Aligned
947	413	Aligned
948	451	Aligned
949	426	Aligned
950	424	Aligned
951	386	Aligned
952	395	Aligned
953	380	Aligned
954	405	Aligned
955	371	Aligned
956	380	Aligned
957	398	Aligned
958	360	Aligned
959	381	Aligned
960	368	Aligned
961	350	Aligned
962	334	Aligned
963	325	Aligned
964	362	Aligned
965	315	Aligned
966	325	Aligned
967	364	Aligned
968	346	Aligned
969	360	Aligned
970	332	Aligned
971	323	Aligned
972	324	Aligned
973	296	Aligned
974	320	Aligned
975	297	Aligned
976	295	Aligned
977	304	Aligned
978	272	Aligned
979	283	Aligned
980	276	Aligned
981	284	Aligned
982	272	Aligned
983	275	Aligned
984	257	Aligned
985	275	Aligned
986	307	Aligned
987	254	Aligned
988	271	Aligned
989	253	Aligned
990	240	Aligned
991	237	Aligned
992	244	Aligned
993	237	Aligned
994	259	Aligned
995	223	Aligned
996	229	Aligned
997	232	Aligned
998	224	Aligned
999	247	Aligned
1000	209	Aligned
1001	206	Aligned
1002	223	Aligned
1003	211	Aligned
1004	211	Aligned
1005	205	Aligned
1006	192	Aligned
1007	212	Aligned
1008	189	Aligned
1009	199	Aligned
1010	218	Aligned
1011	187	Aligned
1012	191	Aligned
1013	190	Aligned
1014	186	Aligned
1015	173	Aligned
1016	183	Aligned
1017	160	Aligned
1018	166	Aligned
1019	177	Aligned
1020	182	Aligned
1021	144	Aligned
1022	152	Aligned
1023	161	Aligned
1024	176	Aligned
1025	156	Aligned
1026	155	Aligned
1027	161	Aligned
1028	140	Aligned
1029	171	Aligned
1030	141	Aligned
1031	159	Aligned
1032	140	Aligned
1033	152	Aligned
1034	143	Aligned
1035	159	Aligned
1036	160	Aligned
1037	126	Aligned
1038	133	Aligned
1039	133	Aligned
1040	119	Aligned
1041	147	Aligned
1042	118	Aligned
1043	115	Aligned
1044	142	Aligned
1045	140	Aligned
1046	114	Aligned
1047	115	Aligned
1048	129	Aligned
1049	129	Aligned
1050	104	Aligned
1051	112	Aligned
1052	107	Aligned
1053	99	Aligned
1054	113	Aligned
1055	101	Aligned
1056	113	Aligned
1057	87	Aligned
1058	108	Aligned
1059	121	Aligned
1060	115	Aligned
1061	97	Aligned
1062	86	Aligned
1063	85	Aligned
1064	79	Aligned
1065	84	Aligned
1066	99	Aligned
1067	89	Aligned
1068	76	Aligned
1069	82	Aligned
1070	75	Aligned
1071	74	Aligned
1072	68	Aligned
1073	75	Aligned
1074	82	Aligned
1075	80	Aligned
1076	77	Aligned
1077	89	Aligned
1078	79	Aligned
1079	71	Aligned
1080	94	Aligned
1081	68	Aligned
1082	65	Aligned
1083	52	Aligned
1084	52	Aligned
1085	80	Aligned
1086	63	Aligned
1087	56	Aligned
1088	60	Aligned
1089	50	Aligned
1090	59	Aligned
1091	53	Aligned
1092	53	Aligned
1093	50	Aligned
1094	56	Aligned
1095	38	Aligned
1096	65	Aligned
1097	53	Aligned
1098	52	Aligned
1099	52	Aligned
1100	46	Aligned
1101	53	Aligned
1102	57	Aligned
1103	38	Aligned
1104	56	Aligned
1105	54	Aligned
1106	35	Aligned
1107	44	Aligned
1108	29	Aligned
1109	53	Aligned
1110	53	Aligned
1111	30	Aligned
1112	38	Aligned
1113	29	Aligned
1114	43	Aligned
1115	36	Aligned
1116	35	Aligned
1117	44	Aligned
1118	56	Aligned
1119	31	Aligned
1120	28	Aligned
1121	33	Aligned
1122	30	Aligned
1123	37	Aligned
1124	32	Aligned
1125	30	Aligned
1126	23	Aligned
1127	22	Aligned
1128	33	Aligned
1129	32	Aligned
1130	26	Aligned
1131	22	Aligned
1132	27	Aligned
1133	21	Aligned
1134	23	Aligned
1135	20	Aligned
1136	24	Aligned
1137	32	Aligned
1138	28	Aligned
1139	22	Aligned
1140	24	Aligned
1141	22	Aligned
1142	24	Aligned
1143	21	Aligned
1144	27	Aligned
1145	19	Aligned
1146	25	Aligned
1147	18	Aligned
1148	27	Aligned
1149	18	Aligned
1150	14	Aligned
1151	21	Aligned
1152	16	Aligned
1153	23	Aligned
1154	15	Aligned
1155	18	Aligned
1156	21	Aligned
1157	17	Aligned
1158	19	Aligned
1159	22	Aligned
1160	13	Aligned
1161	15	Aligned
1162	13	Aligned
1163	16	Aligned
1164	16	Aligned
1165	7	Aligned
1166	8	Aligned
1167	13	Aligned
1168	12	Aligned
1169	14	Aligned
1170	12	Aligned
1171	13	Aligned
1172	8	Aligned
1173	7	Aligned
1174	12	Aligned
1175	11	Aligned
1176	8	Aligned
1177	16	Aligned
1178	9	Aligned
1179	5	Aligned
1180	9	Aligned
1181	14	Aligned
1182	12	Aligned
1183	11	Aligned
1184	6	Aligned
1185	6	Aligned
1186	9	Aligned
1187	5	Aligned
1188	6	Aligned
1189	11	Aligned
1190	13	Aligned
1191	4	Aligned
1192	4	Aligned
1193	4	Aligned
1194	5	Aligned
1195	7	Aligned
1196	4	Aligned
1197	10	Aligned
1198	8	Aligned
1199	9	Aligned
1200	5	Aligned
1201	4	Aligned
1202	3	Aligned
1203	8	Aligned
1204	7	Aligned
1205	4	Aligned
1206	4	Aligned
1207	7	Aligned
1208	2	Aligned
1209	9	Aligned
1210	4	Aligned
1211	3	Aligned
1212	4	Aligned
1213	4	Aligned
1214	2	Aligned
1215	7	Aligned
1216	5	Aligned
1217	4	Aligned
1218	2	Aligned
1219	3	Aligned
1220	5	Aligned
1221	2	Aligned
1222	3	Aligned
1223	4	Aligned
1224	1	Aligned
1225	4	Aligned
1226	1	Aligned
1227	3	Aligned
1228	2	Aligned
1229	5	Aligned
1230	4	Aligned
1231	1	Aligned
1232	2	Aligned
1234	1	Aligned
1236	4	Aligned
1237	2	Aligned
1238	1	Aligned
1239	1	Aligned
1240	5	Aligned
1241	4	Aligned
1242	6	Aligned
1243	3	Aligned
1244	6	Aligned
1245	1	Aligned
1246	1	Aligned
1247	4	Aligned
1250	3	Aligned
1251	1	Aligned
1252	1	Aligned
1253	2	Aligned
1254	1	Aligned
1255	1	Aligned
1256	2	Aligned
1257	2	Aligned
1258	2	Aligned
1259	1	Aligned
1261	4	Aligned
1262	2	Aligned
1263	2	Aligned
1265	1	Aligned
1266	1	Aligned
1267	2	Aligned
1268	1	Aligned
1269	2	Aligned
1272	2	Aligned
1276	1	Aligned
1279	2	Aligned
1280	2	Aligned
1281	1	Aligned
1283	4	Aligned
1284	1	Aligned
1285	1	Aligned
1287	1	Aligned
1288	2	Aligned
1290	2	Aligned
1291	2	Aligned
1297	2	Aligned
1298	2	Aligned
1299	1	Aligned
1309	1	Aligned
1312	2	Aligned
1317	2	Aligned
1318	1	Aligned
1328	1	Aligned
1332	1	Aligned
1334	1	Aligned
1339	1	Aligned
1341	1	Aligned
1361	1	Aligned
1408	1	Aligned
1446	1	Aligned
1673	1	Aligned
1674	1	Aligned
1729	1	Aligned
1730	1	Aligned
1751	1	Aligned
1760	1	Aligned
1815	1	Aligned
2385	1	Aligned
2474	1	Aligned
2475	1	Aligned
2481	1	Aligned
2523	1	Aligned
2562	1	Aligned
2572	1	Aligned
2589	1	Aligned
2608	1	Aligned
2633	1	Aligned
2663	1	Aligned
2747	1	Aligned
2791	1	Aligned
2880	1	Aligned
4742	1	Aligned
14097	1	Aligned
31012	1	Aligned
64254	1	Aligned