##One-way ANOVA
library(multcomp)
## Loading required package: mvtnorm
## Loading required package: survival
## Loading required package: TH.data
## Loading required package: MASS
##
## Attaching package: 'TH.data'
## The following object is masked from 'package:MASS':
##
## geyser
attach(cholesterol)
table(trt)
## trt
## 1time 2times 4times drugD drugE
## 10 10 10 10 10
aggregate(response, by=list(trt), FUN=mean)
## Group.1 x
## 1 1time 5.78197
## 2 2times 9.22497
## 3 4times 12.37478
## 4 drugD 15.36117
## 5 drugE 20.94752
aggregate(response, by=list(trt), FUN=sd)
## Group.1 x
## 1 1time 2.878113
## 2 2times 3.483054
## 3 4times 2.923119
## 4 drugD 3.454636
## 5 drugE 3.345003
fit <- aov(response ~ trt)
summary(fit)
## Df Sum Sq Mean Sq F value Pr(>F)
## trt 4 1351.4 337.8 32.43 9.82e-13 ***
## Residuals 45 468.8 10.4
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
library(gplots)
##
## Attaching package: 'gplots'
## The following object is masked from 'package:stats':
##
## lowess
plotmeans(response ~ trt, xlab="Treatment", ylab="Response",
main="Mean Plot\nwith 95% CI")
detach(cholesterol)
##Tukey HSD pairwise group comparisons
TukeyHSD(fit)
## Tukey multiple comparisons of means
## 95% family-wise confidence level
##
## Fit: aov(formula = response ~ trt)
##
## $trt
## diff lwr upr p adj
## 2times-1time 3.44300 -0.6582817 7.544282 0.1380949
## 4times-1time 6.59281 2.4915283 10.694092 0.0003542
## drugD-1time 9.57920 5.4779183 13.680482 0.0000003
## drugE-1time 15.16555 11.0642683 19.266832 0.0000000
## 4times-2times 3.14981 -0.9514717 7.251092 0.2050382
## drugD-2times 6.13620 2.0349183 10.237482 0.0009611
## drugE-2times 11.72255 7.6212683 15.823832 0.0000000
## drugD-4times 2.98639 -1.1148917 7.087672 0.2512446
## drugE-4times 8.57274 4.4714583 12.674022 0.0000037
## drugE-drugD 5.58635 1.4850683 9.687632 0.0030633
par(las=2)
par(mar=c(5, 8, 4, 2))
plot(TukeyHSD(fit))
library(multcomp)
par(mar=c(5,4,6,2))
tuk <- glht(fit, linfct=mcp(trt="Tukey"))
plot(cld(tuk, level=.05),col="lightgrey")
##assesing tetst assumptions
library(car)
## Loading required package: carData
qqPlot(lm(response ~ trt, data=cholesterol),
simulate=TRUE, main="Q-Q plot", laabels=FALSE)
## [1] 19 38
bartlett.test(response ~ trt, data=cholesterol)
##
## Bartlett test of homogeneity of variances
##
## data: response by trt
## Bartlett's K-squared = 0.57975, df = 4, p-value = 0.9653
##outlierTest
library(car)
outlierTest(fit)
## No Studentized residuals with Bonferonni p < 0.05
## Largest |rstudent|:
## rstudent unadjusted p-value Bonferonni p
## 19 2.251149 0.029422 NA
##One-way ANCOVA
data(litter, package="multcomp")
attach(litter)
table(dose)
## dose
## 0 5 50 500
## 20 19 18 17
aggregate(weight, by=list(dose), FUN=mean)
## Group.1 x
## 1 0 32.30850
## 2 5 29.30842
## 3 50 29.86611
## 4 500 29.64647
fit <- aov(weight ~ gesttime + dose)
summary(fit)
## Df Sum Sq Mean Sq F value Pr(>F)
## gesttime 1 134.3 134.30 8.049 0.00597 **
## dose 3 137.1 45.71 2.739 0.04988 *
## Residuals 69 1151.3 16.69
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##Effect
library(effects)
## lattice theme set by effectsTheme()
## See ?effectsTheme for details.
effect("dose", fit)
##
## dose effect
## dose
## 0 5 50 500
## 32.35367 28.87672 30.56614 29.33460
##Multiple comparisons employing user-supplied contrasts
library(multcomp)
contrast <- rbind("no drug vs. drug" = c(3, -1, -1, -1))
summary(glht(fit, linfct=mcp(dose=contrast)))
##
## Simultaneous Tests for General Linear Hypotheses
##
## Multiple Comparisons of Means: User-defined Contrasts
##
##
## Fit: aov(formula = weight ~ gesttime + dose)
##
## Linear Hypotheses:
## Estimate Std. Error t value Pr(>|t|)
## no drug vs. drug == 0 8.284 3.209 2.581 0.012 *
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
## (Adjusted p values reported -- single-step method)
##Testing for homogeneity of regression slopes
library(multcomp)
fit2 <- aov(weight ~ gesttime*dose, data=litter)
summary(fit2)
## Df Sum Sq Mean Sq F value Pr(>F)
## gesttime 1 134.3 134.30 8.289 0.00537 **
## dose 3 137.1 45.71 2.821 0.04556 *
## gesttime:dose 3 81.9 27.29 1.684 0.17889
## Residuals 66 1069.4 16.20
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##visualization
library(HH)
## Loading required package: lattice
## Loading required package: grid
## Loading required package: latticeExtra
## Loading required package: RColorBrewer
## Loading required package: gridExtra
##
## Attaching package: 'HH'
## The following objects are masked from 'package:car':
##
## logit, vif
## The following object is masked from 'package:gplots':
##
## residplot
ancova(weight ~ gesttime + dose, data=litter)
## Analysis of Variance Table
##
## Response: weight
## Df Sum Sq Mean Sq F value Pr(>F)
## gesttime 1 134.30 134.304 8.0493 0.005971 **
## dose 3 137.12 45.708 2.7394 0.049883 *
## Residuals 69 1151.27 16.685
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##Two-way ANOVA
attach(ToothGrowth)
## The following object is masked from litter:
##
## dose
table(supp, dose)
## dose
## supp 0.5 1 2
## OJ 10 10 10
## VC 10 10 10
aggregate(len, by=list(supp, dose), FUN=mean)
## Group.1 Group.2 x
## 1 OJ 0.5 13.23
## 2 VC 0.5 7.98
## 3 OJ 1.0 22.70
## 4 VC 1.0 16.77
## 5 OJ 2.0 26.06
## 6 VC 2.0 26.14
aggregate(len, by=list(supp, dose), FUN=sd)
## Group.1 Group.2 x
## 1 OJ 0.5 4.459709
## 2 VC 0.5 2.746634
## 3 OJ 1.0 3.910953
## 4 VC 1.0 2.515309
## 5 OJ 2.0 2.655058
## 6 VC 2.0 4.797731
fit <- aov(len ~ supp*dose)
summary(fit)
## Df Sum Sq Mean Sq F value Pr(>F)
## supp 1 205.4 205.4 12.317 0.000894 ***
## dose 1 2224.3 2224.3 133.415 < 2e-16 ***
## supp:dose 1 88.9 88.9 5.333 0.024631 *
## Residuals 56 933.6 16.7
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##Visualization
interaction.plot(dose, supp, len, type="b",
col=c("red","blue"), pch=c(16,18),
main="interaction between Dose and Supplement Type")
#gplots
library(gplots)
plotmeans(len ~ interaction(supp, dose, sep=" "),
connect=list(c(1, 3, 5), c(2, 4, 6)),
col=c("red", "darkgreen"),
main="interactionplot with 95% CIs",
xlab="Treatment and Dose Combination")
#HH Package
library(HH)
interaction2wt(len~supp*dose)
##Repeated measures ANOVA with one between- and within-groups factor
w1b1 <- subset(CO2, Treatment=='chilled')
fit <- aov(uptake ~ (conc*Type) + Error(Plant/(conc)), w1b1)
summary(fit)
##
## Error: Plant
## Df Sum Sq Mean Sq F value Pr(>F)
## Type 1 2667.2 2667.2 60.41 0.00148 **
## Residuals 4 176.6 44.1
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Error: Plant:conc
## Df Sum Sq Mean Sq F value Pr(>F)
## conc 1 888.6 888.6 215.46 0.000125 ***
## conc:Type 1 239.2 239.2 58.01 0.001595 **
## Residuals 4 16.5 4.1
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Error: Within
## Df Sum Sq Mean Sq F value Pr(>F)
## Residuals 30 869 28.97
par(las=2)
par(mar=c(10, 4, 4, 2))
with(w1b1, interaction.plot(conc,Type,uptake,
type="b", col=c("red", "blue"), pch=c(16,18),
main="Interaction Plot for Plant Type and Concentration"))
boxplot(uptake ~ Type*conc, data=w1b1, col=(c("gold", "green")),
main="chilled Quebec and Mississippi Plants",
ylab="Carbon dioxide uptake rate (umol/m^2 sec)")
##One-way MANOVA
library(MASS)
attach(UScereal)
y <- cbind(calories, fat, sugars)
aggregate(y, by=list(shelf), FUN=mean)
## Group.1 calories fat sugars
## 1 1 119.4774 0.6621338 6.295493
## 2 2 129.8162 1.3413488 12.507670
## 3 3 180.1466 1.9449071 10.856821
cov(y)
## calories fat sugars
## calories 3895.24210 60.674383 180.380317
## fat 60.67438 2.713399 3.995474
## sugars 180.38032 3.995474 34.050018
fit <-manova(y ~ shelf)
summary(fit)
## Df Pillai approx F num Df den Df Pr(>F)
## shelf 1 0.19594 4.955 3 61 0.00383 **
## Residuals 63
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
summary.aov(fit)
## Response calories :
## Df Sum Sq Mean Sq F value Pr(>F)
## shelf 1 45313 45313 13.995 0.0003983 ***
## Residuals 63 203982 3238
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Response fat :
## Df Sum Sq Mean Sq F value Pr(>F)
## shelf 1 18.421 18.4214 7.476 0.008108 **
## Residuals 63 155.236 2.4641
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Response sugars :
## Df Sum Sq Mean Sq F value Pr(>F)
## shelf 1 183.34 183.34 5.787 0.01909 *
## Residuals 63 1995.87 31.68
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##Assessing multivariate normallty
center <- colMeans(y)
n <- nrow(y)
p <- ncol(y)
cov <- cov(y)
d <- mahalanobis(y,center,cov)
coord <- qqplot(qchisq(ppoints(n),df=p),
d, main="Q-Q Plot Assessing Multivariate Normality",
ylab="Mahalanobis D2")
abline(a=0,b=1)
identify(coord$x, coord$y, labels=row.names(UScereal))
## integer(0)
library(mvoutlier)
## Loading required package: sgeostat
## sROC 0.1-2 loaded
outliers <- aq.plot(y)
## Projection to the first and second robust principal components.
## Proportion of total variation (explained variance): 0.9789888
outliers
## $outliers
## [1] FALSE FALSE FALSE FALSE FALSE FALSE FALSE FALSE FALSE FALSE TRUE
## [12] TRUE FALSE FALSE FALSE FALSE FALSE TRUE FALSE FALSE FALSE FALSE
## [23] FALSE FALSE FALSE FALSE FALSE FALSE FALSE FALSE TRUE TRUE FALSE
## [34] FALSE FALSE FALSE FALSE FALSE FALSE TRUE FALSE FALSE FALSE TRUE
## [45] FALSE FALSE FALSE FALSE FALSE TRUE FALSE FALSE FALSE FALSE FALSE
## [56] FALSE FALSE FALSE FALSE FALSE FALSE FALSE FALSE FALSE FALSE
##Robust one-way MANOVA
library(rrcov)
## Loading required package: robustbase
##
## Attaching package: 'robustbase'
## The following object is masked from 'package:survival':
##
## heart
## Scalable Robust Estimators with High Breakdown Point (version 1.4-3)
Wilks.test(y,shelf,method="mcd")
##
## Robust One-way MANOVA (Bartlett Chi2)
##
## data: x
## Wilks' Lambda = 0.51073, Chi2-Value = 24.4890, DF = 5.0527,
## p-value = 0.0001837
## sample estimates:
## calories fat sugars
## 1 119.8210 0.7010828 5.663143
## 2 128.0407 1.1849576 12.537533
## 3 160.8604 1.6524559 10.352646
##ANOVA as regression
library(multcomp)
levels(cholesterol$trt)
## [1] "1time" "2times" "4times" "drugD" "drugE"
fit.aov<- aov(response ~ trt, data=cholesterol)
summary(fit.aov)
## Df Sum Sq Mean Sq F value Pr(>F)
## trt 4 1351.4 337.8 32.43 9.82e-13 ***
## Residuals 45 468.8 10.4
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
## A regression approach to the ANOVA problem
fit.lm <- lm(response ~ trt, data=cholesterol)
summary(fit.lm)
##
## Call:
## lm(formula = response ~ trt, data = cholesterol)
##
## Residuals:
## Min 1Q Median 3Q Max
## -6.5418 -1.9672 -0.0016 1.8901 6.6008
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 5.782 1.021 5.665 9.78e-07 ***
## trt2times 3.443 1.443 2.385 0.0213 *
## trt4times 6.593 1.443 4.568 3.82e-05 ***
## trtdrugD 9.579 1.443 6.637 3.53e-08 ***
## trtdrugE 15.166 1.443 10.507 1.08e-13 ***
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 3.227 on 45 degrees of freedom
## Multiple R-squared: 0.7425, Adjusted R-squared: 0.7196
## F-statistic: 32.43 on 4 and 45 DF, p-value: 9.819e-13
contrasts(cholesterol$trt)
## 2times 4times drugD drugE
## 1time 0 0 0 0
## 2times 1 0 0 0
## 4times 0 1 0 0
## drugD 0 0 1 0
## drugE 0 0 0 1