Análisis de varianza

Se realizó el análisis de varianza con dos factores y la prueba de comparación de médias Tukey. El ANOVA se realizó para dos factores (Médio de cultivo y época).

DTangelica<-read.csv("DTangelica.csv", sep = ";", header = T)#
summary(DTangelica)
##       trat           epoc         rept          DVIVOS     
##  citrico:8   trimestre3:12   Min.   :1.00   Min.   : 5.00  
##  H2O    :8   Trimestre4:12   1st Qu.:1.75   1st Qu.: 6.60  
##  MS3K   :8                   Median :2.50   Median :11.77  
##                              Mean   :2.50   Mean   :10.69  
##                              3rd Qu.:3.25   3rd Qu.:13.96  
##                              Max.   :4.00   Max.   :14.93  
##      DOXID           PCONT             BACT             HONG       
##  Min.   :0.200   Min.   :0.0000   Min.   :0.0000   Min.   :0.0000  
##  1st Qu.:1.195   1st Qu.:0.0675   1st Qu.:0.0000   1st Qu.:0.0000  
##  Median :1.815   Median :0.1700   Median :0.1300   Median :0.0000  
##  Mean   :1.778   Mean   :0.2521   Mean   :0.1442   Mean   :0.1079  
##  3rd Qu.:2.493   3rd Qu.:0.4000   3rd Qu.:0.2000   3rd Qu.:0.2000  
##  Max.   :3.000   Max.   :0.6000   Max.   :0.6000   Max.   :0.4000  
##      DMUERT         ResDVIVOS         ResDOXIDA         RESPCONT    
##  Min.   :0.0700   Min.   :0.03000   Min.   :0.0500   Min.   :0.100  
##  1st Qu.:0.7525   1st Qu.:0.05750   1st Qu.:0.2800   1st Qu.:0.580  
##  Median :2.2600   Median :0.07000   Median :0.3550   Median :1.610  
##  Mean   :3.8896   Mean   :0.07083   Mean   :0.3371   Mean   :1.194  
##  3rd Qu.:7.2000   3rd Qu.:0.09000   3rd Qu.:0.4275   3rd Qu.:1.810  
##  Max.   :9.6000   Max.   :0.11000   Max.   :0.5800   Max.   :1.810  
##     RESBACT         RESHONGO       RESDMUERT      
##  Min.   :0.030   Min.   :0.420   Min.   :0.03000  
##  1st Qu.:1.670   1st Qu.:2.040   1st Qu.:0.06000  
##  Median :2.285   Median :2.040   Median :0.07000  
##  Mean   :1.965   Mean   :1.853   Mean   :0.07208  
##  3rd Qu.:2.450   3rd Qu.:2.180   3rd Qu.:0.09000  
##  Max.   :2.620   Max.   :2.530   Max.   :0.11000
aov.data = aov(DTangelica$DVIVOS ~ DTangelica$trat*DTangelica$epoc)
summary(aov.data)
##                                 Df Sum Sq Mean Sq F value   Pr(>F)    
## DTangelica$trat                  2  22.50   11.25   15.11 0.000141 ***
## DTangelica$epoc                  1 247.49  247.49  332.36 4.74e-13 ***
## DTangelica$trat:DTangelica$epoc  2  18.84    9.42   12.65 0.000370 ***
## Residuals                       18  13.40    0.74                     
## ---
## Signif. codes:  0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
TukeyHSD(aov.data)
##   Tukey multiple comparisons of means
##     95% family-wise confidence level
## 
## Fit: aov(formula = DTangelica$DVIVOS ~ DTangelica$trat * DTangelica$epoc)
## 
## $`DTangelica$trat`
##                 diff        lwr      upr     p adj
## H2O-citrico  0.04500 -1.0561612 1.146161 0.9940229
## MS3K-citrico 2.07625  0.9750888 3.177411 0.0003915
## MS3K-H2O     2.03125  0.9300888 3.132411 0.0004906
## 
## $`DTangelica$epoc`
##                         diff      lwr      upr p adj
## Trimestre4-trimestre3 6.4225 5.682372 7.162628     0
## 
## $`DTangelica$trat:DTangelica$epoc`
##                                          diff       lwr      upr     p adj
## H2O:trimestre3-citrico:trimestre3      0.2500 -1.689171 2.189171 0.9982544
## MS3K:trimestre3-citrico:trimestre3     4.0500  2.110829 5.989171 0.0000402
## citrico:Trimestre4-citrico:trimestre3  7.8750  5.935829 9.814171 0.0000000
## H2O:Trimestre4-citrico:trimestre3      7.7150  5.775829 9.654171 0.0000000
## MS3K:Trimestre4-citrico:trimestre3     7.9775  6.038329 9.916671 0.0000000
## MS3K:trimestre3-H2O:trimestre3         3.8000  1.860829 5.739171 0.0000899
## citrico:Trimestre4-H2O:trimestre3      7.6250  5.685829 9.564171 0.0000000
## H2O:Trimestre4-H2O:trimestre3          7.4650  5.525829 9.404171 0.0000000
## MS3K:Trimestre4-H2O:trimestre3         7.7275  5.788329 9.666671 0.0000000
## citrico:Trimestre4-MS3K:trimestre3     3.8250  1.885829 5.764171 0.0000829
## H2O:Trimestre4-MS3K:trimestre3         3.6650  1.725829 5.604171 0.0001399
## MS3K:Trimestre4-MS3K:trimestre3        3.9275  1.988329 5.866671 0.0000595
## H2O:Trimestre4-citrico:Trimestre4     -0.1600 -2.099171 1.779171 0.9997992
## MS3K:Trimestre4-citrico:Trimestre4     0.1025 -1.836671 2.041671 0.9999777
## MS3K:Trimestre4-H2O:Trimestre4         0.2625 -1.676671 2.201671 0.9977985
aov.data = aov(DTangelica$DOXID ~ DTangelica$trat*DTangelica$epoc)
summary(aov.data)
##                                 Df Sum Sq Mean Sq F value  Pr(>F)   
## DTangelica$trat                  2  0.320  0.1598   0.348 0.71084   
## DTangelica$epoc                  1  2.083  2.0827   4.534 0.04728 * 
## DTangelica$trat:DTangelica$epoc  2  5.684  2.8418   6.187 0.00901 **
## Residuals                       18  8.268  0.4593                   
## ---
## Signif. codes:  0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
TukeyHSD(aov.data)
##   Tukey multiple comparisons of means
##     95% family-wise confidence level
## 
## Fit: aov(formula = DTangelica$DOXID ~ DTangelica$trat * DTangelica$epoc)
## 
## $`DTangelica$trat`
##                  diff        lwr       upr     p adj
## H2O-citrico   0.27250 -0.5923415 1.1373415 0.7052899
## MS3K-citrico  0.20125 -0.6635915 1.0660915 0.8251140
## MS3K-H2O     -0.07125 -0.9360915 0.7935915 0.9759554
## 
## $`DTangelica$epoc`
##                            diff         lwr      upr     p adj
## Trimestre4-trimestre3 0.5891667 0.007877315 1.170456 0.0472805
## 
## $`DTangelica$trat:DTangelica$epoc`
##                                          diff        lwr       upr
## H2O:trimestre3-citrico:trimestre3      1.4000 -0.1230064 2.9230064
## MS3K:trimestre3-citrico:trimestre3     1.1000 -0.4230064 2.6230064
## citrico:Trimestre4-citrico:trimestre3  1.9400  0.4169936 3.4630064
## H2O:Trimestre4-citrico:trimestre3      1.0850 -0.4380064 2.6080064
## MS3K:Trimestre4-citrico:trimestre3     1.2425 -0.2805064 2.7655064
## MS3K:trimestre3-H2O:trimestre3        -0.3000 -1.8230064 1.2230064
## citrico:Trimestre4-H2O:trimestre3      0.5400 -0.9830064 2.0630064
## H2O:Trimestre4-H2O:trimestre3         -0.3150 -1.8380064 1.2080064
## MS3K:Trimestre4-H2O:trimestre3        -0.1575 -1.6805064 1.3655064
## citrico:Trimestre4-MS3K:trimestre3     0.8400 -0.6830064 2.3630064
## H2O:Trimestre4-MS3K:trimestre3        -0.0150 -1.5380064 1.5080064
## MS3K:Trimestre4-MS3K:trimestre3        0.1425 -1.3805064 1.6655064
## H2O:Trimestre4-citrico:Trimestre4     -0.8550 -2.3780064 0.6680064
## MS3K:Trimestre4-citrico:Trimestre4    -0.6975 -2.2205064 0.8255064
## MS3K:Trimestre4-H2O:Trimestre4         0.1575 -1.3655064 1.6805064
##                                           p adj
## H2O:trimestre3-citrico:trimestre3     0.0822054
## MS3K:trimestre3-citrico:trimestre3    0.2459505
## citrico:Trimestre4-citrico:trimestre3 0.0083352
## H2O:Trimestre4-citrico:trimestre3     0.2583081
## MS3K:Trimestre4-citrico:trimestre3    0.1498112
## MS3K:trimestre3-H2O:trimestre3        0.9875178
## citrico:Trimestre4-H2O:trimestre3     0.8639507
## H2O:Trimestre4-H2O:trimestre3         0.9844898
## MS3K:Trimestre4-H2O:trimestre3        0.9993957
## citrico:Trimestre4-MS3K:trimestre3    0.5174170
## H2O:Trimestre4-MS3K:trimestre3        1.0000000
## MS3K:Trimestre4-MS3K:trimestre3       0.9996286
## H2O:Trimestre4-citrico:Trimestre4     0.4990713
## MS3K:Trimestre4-citrico:Trimestre4    0.6947417
## MS3K:Trimestre4-H2O:Trimestre4        0.9993957
aov.data = aov(DTangelica$DMUERT ~ DTangelica$trat*DTangelica$epoc)
summary(aov.data)
##                                 Df Sum Sq Mean Sq F value   Pr(>F)    
## DTangelica$trat                  2 249.55  124.78 117.397 4.71e-11 ***
## DTangelica$epoc                  1  13.61   13.61  12.801  0.00215 ** 
## DTangelica$trat:DTangelica$epoc  2   1.60    0.80   0.751  0.48602    
## Residuals                       18  19.13    1.06                     
## ---
## Signif. codes:  0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
TukeyHSD(aov.data)
##   Tukey multiple comparisons of means
##     95% family-wise confidence level
## 
## Fit: aov(formula = DTangelica$DMUERT ~ DTangelica$trat * DTangelica$epoc)
## 
## $`DTangelica$trat`
##                 diff       lwr      upr     p adj
## H2O-citrico  2.16625 0.8506709 3.481829 0.0014777
## MS3K-citrico 7.66125 6.3456709 8.976829 0.0000000
## MS3K-H2O     5.49500 4.1794209 6.810579 0.0000000
## 
## $`DTangelica$epoc`
##                           diff       lwr      upr     p adj
## Trimestre4-trimestre3 1.505833 0.6215879 2.390079 0.0021509
## 
## $`DTangelica$trat:DTangelica$epoc`
##                                          diff        lwr       upr
## H2O:trimestre3-citrico:trimestre3      1.6650 -0.6517662  3.981766
## MS3K:trimestre3-citrico:trimestre3     7.0775  4.7607338  9.394266
## citrico:Trimestre4-citrico:trimestre3  0.7825 -1.5342662  3.099266
## H2O:Trimestre4-citrico:trimestre3      3.4500  1.1332338  5.766766
## MS3K:Trimestre4-citrico:trimestre3     9.0275  6.7107338 11.344266
## MS3K:trimestre3-H2O:trimestre3         5.4125  3.0957338  7.729266
## citrico:Trimestre4-H2O:trimestre3     -0.8825 -3.1992662  1.434266
## H2O:Trimestre4-H2O:trimestre3          1.7850 -0.5317662  4.101766
## MS3K:Trimestre4-H2O:trimestre3         7.3625  5.0457338  9.679266
## citrico:Trimestre4-MS3K:trimestre3    -6.2950 -8.6117662 -3.978234
## H2O:Trimestre4-MS3K:trimestre3        -3.6275 -5.9442662 -1.310734
## MS3K:Trimestre4-MS3K:trimestre3        1.9500 -0.3667662  4.266766
## H2O:Trimestre4-citrico:Trimestre4      2.6675  0.3507338  4.984266
## MS3K:Trimestre4-citrico:Trimestre4     8.2450  5.9282338 10.561766
## MS3K:Trimestre4-H2O:Trimestre4         5.5775  3.2607338  7.894266
##                                           p adj
## H2O:trimestre3-citrico:trimestre3     0.2503933
## MS3K:trimestre3-citrico:trimestre3    0.0000002
## citrico:Trimestre4-citrico:trimestre3 0.8855755
## H2O:Trimestre4-citrico:trimestre3     0.0019564
## MS3K:Trimestre4-citrico:trimestre3    0.0000000
## MS3K:trimestre3-H2O:trimestre3        0.0000091
## citrico:Trimestre4-H2O:trimestre3     0.8262004
## H2O:Trimestre4-H2O:trimestre3         0.1917327
## MS3K:Trimestre4-H2O:trimestre3        0.0000001
## citrico:Trimestre4-MS3K:trimestre3    0.0000011
## H2O:Trimestre4-MS3K:trimestre3        0.0011705
## MS3K:Trimestre4-MS3K:trimestre3       0.1295137
## H2O:Trimestre4-citrico:Trimestre4     0.0188263
## MS3K:Trimestre4-citrico:Trimestre4    0.0000000
## MS3K:Trimestre4-H2O:Trimestre4        0.0000060