HuProt CSF Analysis

Preprocessing

Read ../rawdata_huprot/Control_1256587_2000143978.gpr 
Read ../rawdata_huprot/Control_1311449_2000143966.gpr 
Read ../rawdata_huprot/Control_1312497_2000143964.gpr 
Read ../rawdata_huprot/Control_1313058_2000143976.gpr 
Read ../rawdata_huprot/Grade_L_CJ_14434_2000143958.gpr 
Read ../rawdata_huprot/Grade_L_CH_27096_2000155729.gpr 
Read ../rawdata_huprot/Grade_L_CJ_3659_2000143955.gpr 
Read ../rawdata_huprot/Grade_III_CH_15796_2000143692.gpr 
Read ../rawdata_huprot/Grade_III_CJ_11032_2000143957.gpr 
Read ../rawdata_huprot/Grade_III_CJ_27572_2000144442.gpr 
Read ../rawdata_huprot/Grade_III_CJ_9761_2000144443.gpr 
Read ../rawdata_huprot/Grade_III_CK_5121_2000144441.gpr 
Read ../rawdata_huprot/Grade_III_CK_6451_2000144440.gpr 
Read ../rawdata_huprot/Grade_IV_CH_12724_2000143977.gpr 
Read ../rawdata_huprot/Grade_IV_CH_29738_2000143975.gpr 
Read ../rawdata_huprot/Grade_IV_CH_31148_2000143961.gpr 
Read ../rawdata_huprot/Grade_IV_CH_32705_2000155727.gpr 
Read ../rawdata_huprot/Grade_IV_CJ_11291_2000143969.gpr 
Read ../rawdata_huprot/Grade_IV_CJ_12441_2000143979.gpr

Intensity distribution pre normalization

Intensity distribution post normalization

PCA pre normalization

Performing principle component analysis on raw intensities, which have not been background corrected yields no separation for Low or high grade samples from the controls.

PCA post normalization

MDS post normalization

Heatmap post normalization

Differential Expression

Since the sample size is too less, we do not remove any samples even though they look like outliers on the MDS plot.

targets.protoarray <- readTargets('../rawdata/Protoarray_annotation.csv', sep=',')

targets.protoarray

targets

Conclusion

The table above represents the number of DE genes (adjusted p-value < 0.01) for protoarray and huprot assays. The ‘common’ column represents the number of common genes. ‘jaccard’ is a similarity metric.

We do not see ANY overlap between the two assays. There are two chief reasons:

The experiment is not balanaced: there are different number of samples involved at each level: control, grade2, grade3, grade4.
Sample size is too small: As evident from the PCA and MDS plots, there is too much heterogenity intra-grade and among controls.

Ideally point #2 should not affect the similarity we expect between huprot and protoarray results, but probably problems arising from #1 overshadow it.

At present, the results make little sense since there is no overlap at all.

Boxplots

---
title: "HuProt CSF Analysis"
author: "Saket Choudhary"
output:
  html_notebook:
    fig_height: 30
    fig_width: 30
  html_document: default
  pdf_document: default
date: "04/17/2017"
---

## Preprocessing
```{r, echo=FALSE, warning=FALSE, error=FALSE, message=FALSE}
library(limma)
library(ggplot2)
library(RColorBrewer)
library(cowplot)
library(pheatmap)
```


```{r, echo=FALSE, warning=FALSE, error=FALSE, message=FALSE}
plotHeatMap <- function(y, condition){
  sampleDists <- dist(t(log2(y$E)))
  sampleDistMatrix <- as.matrix(sampleDists)
  rownames(sampleDistMatrix) <- paste(condition, colnames(y$E), sep="-")
  colnames(sampleDistMatrix) <- NULL
  colors <- colorRampPalette( rev(brewer.pal(9, "Blues")) )(255)
  pheatmap(sampleDistMatrix,
           clustering_distance_rows=sampleDists,
          clustering_distance_cols=sampleDists,
           col=colors)
}
```

```{r, echo=FALSE, warning=FALSE, error=FALSE, message=FALSE}
targets <- readTargets('../rawdata_huprot/huprot_common.csv', sep=',')
targets$Condition <- as.factor(targets$Condition)
targetColors <-
    with(targets,
         data.frame(Condition = levels(Condition),
                    color = I(brewer.pal(nlevels(Condition), name = 'Dark2'))))
x <- read.maimages(targets$FileName,
                   path=file.path('..', 'rawdata_huprot'),
                   source='genepix',
                   columns=list(G='F635 Mean', Gb='B635 Median'),
                   green.only=TRUE)

x$genes$Name <- gsub('\\n', '', x$genes$Name)
```


```{r, echo=FALSE, warning=FALSE, error=FALSE, message=FALSE}
negative.controls <- x$genes$Name %in% c('BSA', 
                                         'HeLa cell lysates',
                                         'p300-BHC')

positive.controls <- x$genes$Name %in% c('H2A', 
                                         'H2B', 
                                          'H3', 
                                          'H4', 
                                          'GST10n',
                                          'GST50n',
                                          'GST100n', 
                                          'GST500n')
#par(mfrow=c(1,2))
par(mar=c(8,4,4,4)+.1)

boxplot(log2(x$E[negative.controls,]),las=2, main="Negative Controls raw intensities",ylab="log2-intensity", cex.axis=0.7)
par(mar=c(8,4,4,4)+.1)

boxplot(log2(x$E[positive.controls,]),las=2, main="Positive Controls raw intensities",ylab="log2-intensity", cex.axis=0.7)
```

## Intensity distribution pre normalization


```{r, echo=FALSE, warning=FALSE, error=FALSE, message=FALSE}
x$genes$Status  <- rep('regular', nrow(x))
x$genes$Status[positive.controls] <- 'POSITIVE'
x$genes$Status[negative.controls] <- 'NEGATIVE'
x$genes$ID <- gsub('\\..*', '', x$genes$ID)
y <- neqc(x)

par(mar=c(8,4,4,4)+.1)

boxplot(log2(x$E),las=2, main="Pre-normalization intensities",ylab="log2-intensity", col=targetColors$color[match(targets[colnames(x$E),]$Condition, targetColors$Condition)], pch=19, cex.axis=0.7, cex=0.7)
```

## Intensity distribution post normalization

```{r, echo=FALSE, warning=FALSE, error=FALSE, message=FALSE}
par(mar=c(8,4,4,4)+.1)
boxplot(log2(y$E),las=2, main="Post-normalization intensities",ylab="log2-intensity", col=targetColors$color[match(targets[colnames(x$E),]$Condition, targetColors$Condition)], pch=19, cex.axis=0.7, cex=0.7)
```

## PCA pre normalization

Performing principle component analysis on raw intensities, which have not been background corrected yields no separation for Low or high grade samples from the controls.


```{r, echo=FALSE, warning=FALSE, error=FALSE, message=FALSE}
opar <- par(pch = 19)

pc = prcomp( t( x$E  ), scale=TRUE )
pc.summary <- summary(pc)
pc1.var <- pc.summary$importance[2,1]
pc2.var <- pc.summary$importance[2,2]

plot(pc$x[, 1:2], 
     col=targetColors$color[match(targets$Condition, targetColors$Condition)], 
     cex=1.5, 
     xlab = paste('PC1: ', 100*pc1.var, '%', sep=''), 
     ylab =  paste('PC2: ', 100*pc2.var, '%', sep=''),
     main= "PCA on raw intensities")

legend(x = 'bottomright', 
       legend = as.character(targetColors$Condition),
       col = targetColors$color, pch = par("pch"), bty = 'n', xjust = 1)

```


## PCA post normalization

```{r, echo=FALSE, warning=FALSE, error=FALSE, message=FALSE}
opar <- par(pch = 19)

pc = prcomp( t( y$E  ), scale=TRUE )
pc.summary <- summary(pc)
pc1.var <- pc.summary$importance[2,1]
pc2.var <- pc.summary$importance[2,2]

plot(pc$x[, 1:2], col=targetColors$color[match(targets$Condition, targetColors$Condition)], cex=1.5, xlab = paste('PC1: ', 100*pc1.var, '%', sep=''), ylab =  paste('PC2: ', 100*pc2.var, '%', sep=''),     main= "PCA on background corrected and normalized intensities")
legend(x = 'topright', 
       legend = as.character(targetColors$Condition),
       col = targetColors$color, pch = par("pch"), bty = 'n', xjust = 1)
```


## MDS post normalization


```{r, echo=FALSE, warning=FALSE, error=FALSE, message=FALSE}
opar <- par(pch = 19)
plotMDS(y,  col=targetColors$color[match(targets$Condition, targetColors$Condition)], cex=1.2, pch=19)
legend(x = 'topright', 
       legend = as.character(targetColors$Condition),
       col = targetColors$color, pch = par("pch"), bty = 'n', xjust = 1)

```

## Heatmap post normalization
```{r, echo=FALSE, warning=FALSE, error=FALSE, message=FALSE}
plotHeatMap(y, targets$Condition)
```


## Differential Expression

Since the sample size is too less, we do not remove any samples even though they look like outliers on the MDS plot.


```{r, echo=FALSE, warning=FALSE, error=FALSE, message=FALSE}
 f <- factor(targets$Condition, levels = unique(targets$Condition))
 design <- model.matrix(~0 + f)
 colnames(design) <- levels(f)
```


```{r, echo=FALSE, warning=FALSE, error=FALSE, message=FALSE}
corfit <- duplicateCorrelation(y,
                               design,
                               ndups=2,
                               spacing=1
                               )
fit <- lmFit(y,
             design,
             ndups=2,
             correlation=corfit$consensus.correlation)

contrast.matrix <- makeContrasts(Grade2vsGrade1=Grade2-Grade1, 
                                 Grade3vsGrade2=Grade3-Grade2, 
                                 Grade4vsGrade3=Grade4-Grade3,
                                 Grade1vsControl=Grade1-Control,
                                 Grade2vsControl=Grade2-Control,
                                 Grade3vsControl=Grade3-Control,
                                 Grade4vsControl=Grade4-Control,                              GradevsControl=(Grade1+Grade2+Grade3+Grade4)/4-Control,
                              HighGradevsControl=(Grade3+Grade4)/2-Control,
                              LowGradevsControl=(Grade1+Grade2)/2-Control,
                  HighGradevsLowGrade=(Grade3+Grade4)/2-(Grade1+Grade2)/2,
                                 Grade3vsLowGrade=Grade3-(Grade1+Grade2)/2,
                                 Grade4vsLowGrade=Grade4-(Grade1+Grade2)/2,
                                 levels=design)
fit2 <- contrasts.fit(fit, contrast.matrix)
fit2 <- eBayes(fit2)
coefs <- c('Grade2vsGrade1',
           'Grade3vsGrade2',
           'Grade4vsGrade3',
           'Grade1vsControl',
           'Grade2vsControl',
           'Grade3vsControl',
           'Grade4vsControl',
           'GradevsControl',
           'HighGradevsControl',
           'LowGradevsControl',
           'HighGradevsLowGrade',
           'Grade3vsLowGrade',
           'Grade4vsLowGrade')
expressionValues <- as.data.frame(y$E)
write.table(expressionValues, 
            file.path('..',  'results_huprot_04162017',paste('huprot_normalized_values','csv', sep='.') ))
for (coef in coefs){
  tt <- topTable(fit2, coef=coef, number = Inf)
  tt.sig <- subset(tt, tt$adj.P.Val<0.1)
  write.table(tt, file.path('..', 'results_huprot_04162017', paste(coef,'csv', sep='.') ), row.names = FALSE)
  write.table(tt.sig, file.path('..', 'results_huprot_04162017', 'significant_filtered', paste(coef,'csv', sep='.') ), row.names = FALSE)
}

```







```{r}
targets.protoarray <- readTargets('../rawdata/Protoarray_annotation.csv', sep=',')

```

```{r}
targets.protoarray
```

```{r}
targets
```

## Conclusion
The table above represents the number of DE genes (adjusted p-value < 0.01) for protoarray and huprot assays. The 'common' column represents the number of common genes. 'jaccard' is a similarity metric.

We do not see ANY overlap between the two assays. There are two chief reasons:

1. The experiment is not balanaced: there are different number of samples involved at each level: control, grade2, grade3, grade4. 

2. Sample size is too small: As evident from the PCA and MDS plots, there is too much heterogenity intra-grade and among controls.

Ideally point #2 should not affect the similarity we expect between huprot and protoarray results, but probably problems arising from #1 overshadow it.

At present, the results make little sense since there is no overlap at all.


## Boxplots

