August 3, 2016

Outline

  • Motivation
  • Experimental design: batch effects and confounding
  • Normalization: accounting for sample quality
  • SCONE: An R package to normalize single-cell RNA-seq


slides available at: http://rpubs.com/daviderisso

Illuminating cellular diversity in the brain

The brain is made up of 100’s if not 1000’s different cell types.
We need a rational way to identify and classify them.

Single-cell sequencing of S1 cortex