Instructions:

This document provides data corresponding to the Microbiology 3023L (section 911288) lab exercises of pipetting. The graphs listed below will be accompained with questions for you to answer and submit by email (friveram@mdc.edu) to Prof. Rivera-Mariani no later than Friday March 8th by 11:59 pm. When submitting your answers, in the subject of the email, write Answers to Lab Report (Pipetting Techniques). In the body, for example, for question 1 letter a, write 1.a: and your answer . Write answers in complete sentences in order to be awarded full grade for your answers. The total points of this lab report is 10 points.

1) The histogram above corresponds to the distributions of volumes pipetted during the pipetting lab exercise we performed in class. Recall that in this lab exercise, the professor recorded your three masses of the volumes of water pipetted. Therefore, volume of water will be equal to mass of water. Also recall that the professor establshed a 3% margin of error for your tries when 100 uL of water were pipetted.

a) **From above histogram, what could you say with regard to the overall pipetting of the class? (*Explain*)**.
b) **Do you believe thie histogram provide you enough information to tell if the class was precise or not, or if the class was         accurate or note?**

  1. The boxplot above corresponds to the distribution of volumes, but this time for each student present during the day you were evaluated for your pipetting presicion and accuray. The names of the students were omitted by the processor and instead numbers were assigned. Answer the questions below for this boxplot. Make sure to follow the legend within the graph.

    1. Which student(s) was/were the least precised? (Explain)
    2. Which student(s) was/were the least accurate? (Explain)
    3. Which student(s) was/were the most precise?
    4. Which student(s) was/were the most accurate?
    5. Based on what you know about possible errors while pipetting, what may have been the cause(s) that not all students’ volumes were within the 3% margin of error established by the professor?
## Loading required package: lattice
## Loading required package: survival
## Loading required package: Formula
## Loading required package: ggplot2
## 
## Attaching package: 'Hmisc'
## The following objects are masked from 'package:base':
## 
##     format.pval, round.POSIXt, trunc.POSIXt, units

Note: disregard the messages above the graph as this report was created through a series of programming codes using the open-source software R (https://www.r-project.org/).

  1. The graph above corresponds to a current grade for each students plotted against the mass of the volumes of water pipetted.

    1. Do you believe the volumes of water pipetted were associated current grade of the students in the course? (Explain)?

This document was created with the open-course statistical software R (https://www.r-project.org/). If you would like to take at code for this document, feel free to visit Prof. Rivera-Mariani’ online repository four our course at https://github.com/friveramariani/mcb3023L-2015-2.

## [1] "This document was created for the Microbiology Lab Course (MCB3023L, section 911288) taught at Miami Dade College) by prof. Rivera-Mariani on 2016-04-04 16:03:04"