Meeting X

Aanwezig: X ## Research question 1 How successful is the practice of sowing commercial seed mixtures on rubble-composed soils to create species-rich grasslands, and how is this success affected by soil conditions (pH and Olsen P), numbers of seed in the seed mixtures, and years since initial sowing?

RQ1_Descr

Zie script > RQ1_Descriptive.R & RQ1_models.R

Stephanie: Enkel nog de toevoeging om eventueel ook het verschil tussen de groepen statistisch te testen. (kan met de functies emmeans en daarna cld uit het pakket emmeans, laat me weten als je daar hulp bij wil) Anders ga je geen echte uitspraken kunnen doen als “er zijn gemiddeld gezien meer spontane soorten dan ingezaaide soorten gespot in de proefvlakken”.

TEST DIFFERENCES

Difference between “observed and sown” and “observed and non-sown” #### 1) Model > mod <- lmerTest::lmer(value ~ category + (1|location), data = df_model) > > - Value = number of species/location > - Category = “observed and sown” and “observed and non-sown” > - (1|location) = Compare categories while accounting for the fact that measurements come from the same locations. > > emmeans(mod, pairwise ~ category) > is dit noidg? > ! singular fit warning happened

2) Wilcoxon signed rank test

wilcox.test(df_NS\(n_obs_sown, df_NS\)n_obs_nonsown,paired = TRUE)

MODELS

General: - For each characteristic, we look at three different groups: (i) observed, (ii) observed and non-sown, and (iii) observed and sown - The predictor variables are soil conditions (pH and Olsen P), numbers of seed in the seed mixtures, and years since initial sowing #### Description of predictor variables | Statistic | Number of seeds (seed mixture) | Age | Olsen P | pH | |—|—:|—:|—:|—:| | Mean | 36.04 | 8.02 | 50.39 | 7.65 | | Median | 34.5 | 7 | 49.66 | 7.81 | | Minimum | 10 | 3 | 9.88 | 6.53 | | Maximum | 63 | 17 | 114.82 | 8.20 |

Pred_var

Number of species

How are total, sown, and non-sown plant species richness influenced by soil conditions (pH and Olsen P), seed mixture richness, and years since sowing?

m_obs <- glmmTMB(n_obs ~ scale(sown_mixt) + scale(age) + scale(olsenp_2022) + scale(ph_2022), family = nbinom2, df)

m_obs_nonsown <- glmmTMB(n_nonsown ~ scale(sown_mixt) + scale(age) + scale(olsenp_2022) + scale(ph_2022), family = nbinom2, df)

m_obs_sown <- glmmTMB(n_sown ~ scale(sown_mixt) + scale(age) + scale(olsenp_2022) + scale(ph_2022), family = nbinom2, df)

Do green managers appear to adjust seed mixture richness according to soil characteristics (pH and Olsen P)?

m_sown <- glmmTMB(sown_mixt ~ scale(olsenp_2022) + scale(ph_2022), family = nbinom2, df)

Vegetation cover

Estimates of models are very high - What does this mean?

Grass Cover

Model for grass cover sown - rediduals >> KS TEST “deviation significant” >> a lot of zeros (not a problem with the “number of grass species”).

image

Annual Cover

Model for Annual cover (for all three labels) - rediduals >> KS TEST “deviation significant”

Overview

modelfigure

Research Question 2

What are the functional characteristics of commercial seed mixtures applied in the city of Ghent and of the plant community of urban grasslands established on rubble-composed soils through the sowing of these seed mixtures?

Lander: gewoon beschrijving van de variatie in CSR/Ellenberg in de verschillende pools (sown, etc.) tussen de locaties

Potential Nectar Value (PNV)

Nectar_mean_dif

Mean

dataset Nectar (mean ± sd) Nectar (min–max)
Observed 29.98 ± 9.47 15.37–60.98
Observed & Nonsown 26.61 ± 12.38 1.98–72.51
Observed & Sown 37.17 ± 25.18 0.00–125.00
Sown 34.22 ± 10.06 16.14–67.52

Differences

Category Emmean SE df Lower CL Upper CL Group
Observed 30.0 2.26 188 24.3 35.7 ab
Observed & Nonsown 26.6 2.26 188 20.9 32.3 a
Observed & Sown 37.2 2.26 188 31.5 42.8 b
Sown 34.2 2.26 188 28.5 39.9 ab

CSR

CSR_mean_Diff

Mean

dataset C (mean ± sd) C (min–max) S (mean ± sd) S (min–max) R (mean ± sd) R (min–max)
Observed 29.10 ± 4.85 20.32–45.41 19.92 ± 3.50 11.74–27.44 50.98 ± 4.85 33.70–60.03
Observed and sown 32.38 ± 15.40 0.00–71.82 20.54 ± 13.72 0.00–53.99 36.66 ± 15.42 0.00–71.94
Observed and Non-sown 26.77 ± 6.20 15.92–48.22 19.43 ± 3.58 10.28–26.14 53.80 ± 5.76 35.12–64.15
Sown 32.86 ± 7.35 20.78–52.24 22.82 ± 7.21 2.98–34.24 44.32 ± 3.40 36.28–55.05

Differences

trait group emmean SE lower.CL upper.CL .group
C Observed 29.21 0.63 27.63 30.79 a
C Observed_Nonsown 27.24 0.73 25.42 29.05 a
C Observed_Sown 35.34 1.29 32.14 38.55 b
C Sown 32.51 0.51 31.23 33.79 b
S Observed 19.89 0.65 18.28 21.50 a
S Observed_Nonsown 19.41 0.74 17.55 21.26 a
S Observed_Sown 21.39 1.31 18.11 24.66 ab
S Sown 23.42 0.52 22.11 24.72 b
R Observed 50.90 0.62 49.35 52.46 b
R Observed_Nonsown 53.36 0.72 51.57 55.14 b
R Observed_Sown 43.27 1.27 40.11 46.42 a
R Sown 44.07 0.50 42.82 45.33 a

EIVE

EIVE_mean_Diff

Differences

trait group emmean SE lower.CL upper.CL .group
EIVE_N Observed 5.25 0.04 5.14 5.35 a
EIVE_N Observed_Nonsown 5.47 0.05 5.35 5.59 b
EIVE_N Observed_Sown 4.55 0.09 4.33 4.77 c
EIVE_N Sown 4.63 0.04 4.54 4.72 c
EIVE_M Observed 4.29 0.02 4.23 4.34 a
EIVE_M Observed_Nonsown 4.38 0.03 4.32 4.45 b
EIVE_M Observed_Sown 3.99 0.05 3.88 4.10 c
EIVE_M Sown 3.99 0.02 3.94 4.03 c
EIVE_R Observed 6.02 0.03 5.95 6.09 a
EIVE_R Observed_Nonsown 6.03 0.03 5.95 6.11 a
EIVE_R Observed_Sown 5.97 0.06 5.83 6.11 a
EIVE_R Sown 6.08 0.02 6.03 6.14 a
EIVE_T Observed 4.28 0.01 4.25 4.32 ab
EIVE_T Observed_Nonsown 4.32 0.02 4.28 4.36 b
EIVE_T Observed_Sown 4.16 0.03 4.10 4.23 c
EIVE_T Sown 4.26 0.01 4.23 4.29 a

APPENDIX

Total number of species

TotalNS

Potential Nectar Value

CWM

dataset Nectar (mean ± sd) Nectar (min–max)
Observed 31.21 ± 23.55 2.39–88.07
Observed & Nonsown 21.35 ± 22.32 0.41–111.38
Observed & Sown 45.78 ± 38.35 0.00–125.00

CSR

With “CWM”

\[ \text{CWM}_{CSR} = \sum_{i=1}^{S} (P_i \times T_i) \]

With \(S\) the absolute measure

With \(P_{\text{i}}\) the relative abundance of species i

With \(T_{\text{i}}\) the trait value (CSR) of species i

CWM

dataset C (mean ± sd) C (min–max) S (mean ± sd) S (min–max) R (mean ± sd) R (min–max)
Observed 28.59 ± 10.00 13.38–71.90 24.74 ± 10.42 4.22–57.67 46.68 ± 10.09 16.27–66.20
Observed and sown 31.10 ± 18.53 0.00–71.82 21.26 ± 19.02 0.00–64.38 37.22 ± 17.73 0.00–85.95
Observed and Non-sown 26.37 ± 11.98 10.26–77.92 24.59 ± 10.36 3.75–48.64 49.04 ± 11.75 10.53–68.63

CWM (contribution)

With “CWM (contribution)”

\[ X_{\text{total}} = X_{\text{sown}} + X_{\text{non-sown}} \]

dataset C (mean ± sd) C (min–max) S (mean ± sd) S (min–max) R (mean ± sd) R (min–max)
Observed 28.59 ± 10.00 13.38–71.90 24.74 ± 10.42 4.22–57.67 46.68 ± 10.09 16.27–66.20
Observed and sown 11.06 ± 10.44 0.07–34.72 8.09 ± 10.51 0.00–55.86 13.72 ± 11.45 0.08–39.72
Observed and Non-sown 18.68 ± 11.36 2.77–67.14 17.49 ± 10.67 1.72–48.43 34.39 ± 16.09 5.63–64.30

EIVE

With “CWM (contribution)”

\[ X_{\text{total}} = X_{\text{sown}} + X_{\text{non-sown}} \]

With “CWM”

\[ \text{CWM}_{CSR} = \sum_{i=1}^{S} (P_i \times T_i) \]

With \(S\) the absolute measure

With \(P_{\text{i}}\) the relative abundance of species i

With \(T_{\text{i}}\) the trait value (CSR) of species i

EIVE_Mean_CMW_CMWadd