TAC-deTAC Circular Analysis
2026-04-22
Each tab can be clicked on to show off related statistics. All statistics have been computed using RStudio and all data was collected on VevoLab VevoStrain 2.0.
Results
Variable Definitions
| Variable_Name | Definition | Unit |
|---|---|---|
| mouse_ID | Label for each lab mouse | NA |
| time_point | time point and treatment group type | NA |
| true_mitral | 4DUS measured mitral valve diameter | mm |
| calc_mitral | Rounded mitral valve diameter used in VevoLab calculation | mm |
| true_aortic | 4DUS measured aortic valve diameter | mm |
| calc_aortic | Rounded aortic valve diameter used in VevoLab calculation | mm |
| sys_pk | Peak HDF value during systole | % |
| ewave_long_rms | Longitudinal RMS HDF value during diastolic E-wave | % |
| ewave_ratio_rms | Longitudinal to transverse RMS HDF value during diastolic E-wave | % |
| ewave_angle | Average HDF angle during diastolic E-wave | ° |
| cycle_length | Length of the entire heartbeat cycle | ms |
| cycle_long_rms | Longitudinal RMS HDF value during the entire heartbeat | % |
| cycle_trans_rms | Transverse RMS HDF value during the entire heartbeat | % |
| cycle_ratio_rms | Longitudinal to transverse RMS HDF value over the entire heartbeat | % |
| cycle_angle | Average HDF angle over the entire heartbeat | ° |
| sys_length | Length of systole | ms |
| sys_im_length | Length of systolic impulse | ms |
| sys_im_angle | Average HDF angle during systolic impulse | ° |
| sys_im_AUC | Area under the longitudinal curve during systolic impulse | % |
| sys_im_long_rms | Longitudinal RMS HDF value during the systolic impulse | % |
| sys_im_trans_rms | Transverse RMS HDF value during the systolic impulse | % |
| isys_im_length | Length of initial portion of systolic impulse | ms |
| isys_im_long_rms | Longitudinal RMS HDF value during the initial systolic impulse | % |
| dsys_im_length | Length of descending portion of systolic impulse | ms |
| dsys_im_long_rms | Longitudinal RMS HDF value during the descending systolic impulse | % |
| lsys_long_rms | Longitudinal RMS HDF value during the late systole period | % |
| myo_GCS | Myocardial global circumferential strain rate | % |
| myo_GLS | Myocardial global longitudinal strain rate | % |
| endo_GCS | Endocardial global circumferential strain rate | % |
| endo_GLS | Endocardial global longitudinal strain rate | % |
| LV_EF | LV Ejection fraction | % |
| pslax_avg_strain_rate | Parasternal long axis LV average strain rate | d%/dt |
| LV_mass_cor | LV mass corrected mass | mg |
| LVAW_sys | LV anterior wall average thickness during systole | mm |
| LVAW_dias | LV anterior wall average thickness during diastole | mm |
| LVPW_sys | LV posterior wall average thickness during systole | mm |
| LVPW_dias | LV posterior wall average thickness during diastole | mm |
| sax_avg_strain | LV short axis average strain | % |
| LA_EF | LA ejection fraction | % |
| LA_edv | LA end diastolic volume | mL |
| LA_esv | LA end systolic volume | mL |
| LA_strain_mean | LA mean strain | % |
| Ao_pk_vel | Peak aoritc blood flow velocity | mm/s |
| mitral_EA_ratio | Mitral valve blood flow velocity diastolic E-wave to A-wave ratio | NA |
| sys_length_norm | Normalized systole length as a ratio of R to R | % |
| sys_im_length_norm | Normalized systolic impulse length as a ratio of R to R | % |
| isys_im_length_norm | Normalized initial systolic impulse length as a ratio of R to R | % |
| dsys_im_length_norm | Normalized descending systolic impulse length as a ratio of R to R | % |
| isys_dsys_length_ratio | Ratio of initial systolic impulse length to descending systolic impulse length | % |
| isys_dsys_long_ratio | Ratio of initial systolic impulse longitudinal HDF to descending systolic impulse longitudinal HDF | % |
Figure 2
Normality
## === Analyzing: true_mitral ===
## Traditional P: 0.1822277 | LME P: 0.1502281
##
## === Analyzing: true_aortic ===
## Traditional P: 0.01956304 | LME P: 0.01273569
##
## === Analyzing: sys_pk ===
## Traditional P: 0.0004608214 | LME P: 0.0006743964
##
## === Analyzing: ewave_long_rms ===
## Traditional P: 0.1963122 | LME P: 0.201791
##
## === Analyzing: ewave_ratio_rms ===
## Traditional P: 0.5622407 | LME P: 0.4509752
##
## === Analyzing: ewave_angle ===
## Traditional P: 0.5914906 | LME P: 0.5142543
##
## === Analyzing: cycle_length ===## boundary (singular) fit: see help('isSingular')## Traditional P: 0.1885478 | LME P: 0.5015464
##
## === Analyzing: cycle_long_rms ===## boundary (singular) fit: see help('isSingular')## Traditional P: 0.01496467 | LME P: 0.01496467
##
## === Analyzing: cycle_trans_rms ===## boundary (singular) fit: see help('isSingular')## Traditional P: 0.9931357 | LME P: 0.9931357
##
## === Analyzing: cycle_ratio_rms ===
## Traditional P: 0.1751387 | LME P: 0.07405503
##
## === Analyzing: cycle_angle ===
## Traditional P: 0.1928965 | LME P: 0.1603796
##
## === Analyzing: sys_length ===## boundary (singular) fit: see help('isSingular')## Traditional P: 0.7026288 | LME P: 0.7026288
##
## === Analyzing: sys_im_length ===## boundary (singular) fit: see help('isSingular')## Traditional P: 0.2797528 | LME P: 0.2797528
##
## === Analyzing: sys_im_angle ===## boundary (singular) fit: see help('isSingular')## Traditional P: 0.1335637 | LME P: 0.1335637
##
## === Analyzing: sys_im_AUC ===
## Traditional P: 0.0003923288 | LME P: 0.00152643
##
## === Analyzing: sys_im_long_rms ===
## Traditional P: 3.280456e-05 | LME P: 0.0001312536
##
## === Analyzing: sys_im_trans_rms ===## boundary (singular) fit: see help('isSingular')## Traditional P: 0.2290432 | LME P: 0.1558394
##
## === Analyzing: isys_im_length ===## boundary (singular) fit: see help('isSingular')## Traditional P: 0.0006819497 | LME P: 0.0006819497
##
## === Analyzing: isys_im_long_rms ===## boundary (singular) fit: see help('isSingular')## Traditional P: 0.0003337566 | LME P: 0.0003337566
##
## === Analyzing: dsys_im_length ===
## Traditional P: 6.527215e-05 | LME P: 0.0001717036
##
## === Analyzing: dsys_im_long_rms ===
## Traditional P: 0.000393669 | LME P: 0.0001666867
##
## === Analyzing: lsys_long_rms ===## boundary (singular) fit: see help('isSingular')## Traditional P: 0.04148182 | LME P: 0.03376443
##
## === Analyzing: myo_GCS ===
## Traditional P: 5.71203e-05 | LME P: 9.590857e-05
##
## === Analyzing: myo_GLS ===
## Traditional P: 5.491679e-06 | LME P: 5.173217e-06
##
## === Analyzing: endo_GCS ===
## Traditional P: 0.000470916 | LME P: 0.0001619262
##
## === Analyzing: endo_GLS ===
## Traditional P: 1.129429e-06 | LME P: 1.743836e-06
##
## === Analyzing: LV_EF ===
## Traditional P: 7.465683e-06 | LME P: 1.671635e-05
##
## === Analyzing: pslax_avg_strain_rate ===## boundary (singular) fit: see help('isSingular')## Traditional P: 0.1385494 | LME P: 0.1385494
##
## === Analyzing: LV_mass_cor ===## boundary (singular) fit: see help('isSingular')## Traditional P: 0.001028144 | LME P: 1.1136e-05
##
## === Analyzing: LVAW_sys ===## boundary (singular) fit: see help('isSingular')## Traditional P: 0.3733527 | LME P: 0.4769215
##
## === Analyzing: LVAW_dias ===
## Traditional P: 0.005862358 | LME P: 0.006097522
##
## === Analyzing: LVPW_sys ===
## Traditional P: 0.8715581 | LME P: 0.7380513
##
## === Analyzing: LVPW_dias ===
## Traditional P: 0.01346732 | LME P: 0.07299802
##
## === Analyzing: sax_avg_strain ===
## Traditional P: 2.041868e-05 | LME P: 2.152344e-05
##
## === Analyzing: Ao_pk_vel ===
## Traditional P: 3.571285e-05 | LME P: 2.889517e-11
##
## === Analyzing: sys_length_norm ===
## Traditional P: 0.2770258 | LME P: 0.28588
##
## === Analyzing: sys_im_length_norm ===## boundary (singular) fit: see help('isSingular')## Traditional P: 0.1221507 | LME P: 0.09937781
##
## === Analyzing: isys_im_length_norm ===## boundary (singular) fit: see help('isSingular')## Traditional P: 0.0006399792 | LME P: 0.0006399792
##
## === Analyzing: dsys_im_length_norm ===
## Traditional P: 0.001391323 | LME P: 0.0002674289
##
## === Analyzing: isys_dsys_length_ratio ===## boundary (singular) fit: see help('isSingular')## Traditional P: 0.0009775795 | LME P: 0.0009775795
##
## === Analyzing: isys_dsys_long_ratio ===
## Traditional P: 2.576261e-06 | LME P: 3.256376e-06
##
## === Analyzing: mitral_EA_ratio ===
## Traditional P: 0.03885208 | LME P: 0.2258897## Variable Traditional_P LME_P
## Ao_pk_vel Ao_pk_vel 3.571285e-05 2.889517e-11
## endo_GLS endo_GLS 1.129429e-06 1.743836e-06
## isys_dsys_long_ratio isys_dsys_long_ratio 2.576261e-06 3.256376e-06
## myo_GLS myo_GLS 5.491679e-06 5.173217e-06
## LV_mass_cor LV_mass_cor 1.028144e-03 1.113600e-05
## LV_EF LV_EF 7.465683e-06 1.671635e-05
## sax_avg_strain sax_avg_strain 2.041868e-05 2.152344e-05
## myo_GCS myo_GCS 5.712030e-05 9.590857e-05
## sys_im_long_rms sys_im_long_rms 3.280456e-05 1.312536e-04
## endo_GCS endo_GCS 4.709160e-04 1.619262e-04
## dsys_im_long_rms dsys_im_long_rms 3.936690e-04 1.666867e-04
## dsys_im_length dsys_im_length 6.527215e-05 1.717036e-04
## dsys_im_length_norm dsys_im_length_norm 1.391323e-03 2.674289e-04
## isys_im_long_rms isys_im_long_rms 3.337566e-04 3.337566e-04
## isys_im_length_norm isys_im_length_norm 6.399792e-04 6.399792e-04
## sys_pk sys_pk 4.608214e-04 6.743964e-04
## isys_im_length isys_im_length 6.819497e-04 6.819497e-04
## isys_dsys_length_ratio isys_dsys_length_ratio 9.775795e-04 9.775795e-04
## sys_im_AUC sys_im_AUC 3.923288e-04 1.526430e-03
## LVAW_dias LVAW_dias 5.862358e-03 6.097522e-03
## true_aortic true_aortic 1.956304e-02 1.273569e-02
## cycle_long_rms cycle_long_rms 1.496467e-02 1.496467e-02
## lsys_long_rms lsys_long_rms 4.148182e-02 3.376443e-02
## LVPW_dias LVPW_dias 1.346732e-02 7.299802e-02
## cycle_ratio_rms cycle_ratio_rms 1.751387e-01 7.405503e-02
## sys_im_length_norm sys_im_length_norm 1.221507e-01 9.937781e-02
## sys_im_angle sys_im_angle 1.335637e-01 1.335637e-01
## pslax_avg_strain_rate pslax_avg_strain_rate 1.385494e-01 1.385494e-01
## true_mitral true_mitral 1.822277e-01 1.502281e-01
## sys_im_trans_rms sys_im_trans_rms 2.290432e-01 1.558394e-01
## cycle_angle cycle_angle 1.928965e-01 1.603796e-01
## ewave_long_rms ewave_long_rms 1.963122e-01 2.017910e-01
## mitral_EA_ratio mitral_EA_ratio 3.885208e-02 2.258897e-01
## sys_im_length sys_im_length 2.797528e-01 2.797528e-01
## sys_length_norm sys_length_norm 2.770258e-01 2.858800e-01
## ewave_ratio_rms ewave_ratio_rms 5.622407e-01 4.509752e-01
## LVAW_sys LVAW_sys 3.733527e-01 4.769215e-01
## cycle_length cycle_length 1.885478e-01 5.015464e-01
## ewave_angle ewave_angle 5.914906e-01 5.142543e-01
## sys_length sys_length 7.026288e-01 7.026288e-01
## LVPW_sys LVPW_sys 8.715581e-01 7.380513e-01
## cycle_trans_rms cycle_trans_rms 9.931357e-01 9.931357e-01
## Method_Used
## Ao_pk_vel Kruskal-Wallis
## endo_GLS ANOVA
## isys_dsys_long_ratio ANOVA
## myo_GLS ANOVA
## LV_mass_cor Kruskal-Wallis
## LV_EF ANOVA
## sax_avg_strain ANOVA
## myo_GCS ANOVA
## sys_im_long_rms ANOVA
## endo_GCS Kruskal-Wallis
## dsys_im_long_rms Kruskal-Wallis
## dsys_im_length ANOVA
## dsys_im_length_norm Kruskal-Wallis
## isys_im_long_rms ANOVA
## isys_im_length_norm ANOVA
## sys_pk Kruskal-Wallis
## isys_im_length ANOVA
## isys_dsys_length_ratio ANOVA
## sys_im_AUC Kruskal-Wallis
## LVAW_dias Kruskal-Wallis
## true_aortic ANOVA
## cycle_long_rms ANOVA
## lsys_long_rms Kruskal-Wallis
## LVPW_dias Kruskal-Wallis
## cycle_ratio_rms Kruskal-Wallis
## sys_im_length_norm Kruskal-Wallis
## sys_im_angle ANOVA
## pslax_avg_strain_rate ANOVA
## true_mitral ANOVA
## sys_im_trans_rms Kruskal-Wallis
## cycle_angle ANOVA
## ewave_long_rms ANOVA
## mitral_EA_ratio Kruskal-Wallis
## sys_im_length ANOVA
## sys_length_norm ANOVA
## ewave_ratio_rms ANOVA
## LVAW_sys Kruskal-Wallis
## cycle_length Kruskal-Wallis
## ewave_angle ANOVA
## sys_length ANOVA
## LVPW_sys Kruskal-Wallis
## cycle_trans_rms ANOVASignificance Between Groups
Figure 3
Circular Statistics
## Warning: package 'circular' was built under R version 4.4.3##
## Attaching package: 'circular'## The following object is masked from 'package:pROC':
##
## var## The following objects are masked from 'package:stats':
##
## sd, var## Warning in as.circular(clean_data[[col_name]], units = "degrees", template = "none", : an object is coerced to the class 'circular' using default value for the following components:
## type: 'angles'
## zero: 0
## rotation: 'counter'
## evalexprenvirenclos## Warning in as.circular(clean_data[[col_name]], units = "degrees", template = "none", : an object is coerced to the class 'circular' using default value for the following components:
## type: 'angles'
## zero: 0
## rotation: 'counter'
## evalexprenvirenclos
## Warning in as.circular(clean_data[[col_name]], units = "degrees", template = "none", : an object is coerced to the class 'circular' using default value for the following components:
## type: 'angles'
## zero: 0
## rotation: 'counter'
## evalexprenvirenclos## Warning in watson.williams.test.default(x = angles, group = groups_factor):
## Concentration parameters ( 389, 105, 3335) not equal between groups. The test
## might not be applicable## Warning in as.circular(clean_data$sys_im_angle[clean_data$study_type == : an object is coerced to the class 'circular' using default value for the following components:
## type: 'angles'
## template: 'none'
## modulo: 'asis'
## zero: 0
## rotation: 'counter'
## plot.circularas.circular(clean_data$sys_im_angle[clean_data$study_type == "tac"], units = "degrees")TRUE18TAC Momentum Distribution
## Warning in as.circular(clean_data[[col_name]], units = "degrees", modulo = "2pi"): an object is coerced to the class 'circular' using default value for the following components:
## type: 'angles'
## template: 'none'
## zero: 0
## rotation: 'counter'
## evalexprenvirenclos## Warning in as.circular(clean_data[[col_name]], units = "degrees", modulo = "2pi"): an object is coerced to the class 'circular' using default value for the following components:
## type: 'angles'
## template: 'none'
## zero: 0
## rotation: 'counter'
## evalexprenvirenclos
## Warning in as.circular(clean_data[[col_name]], units = "degrees", modulo = "2pi"): an object is coerced to the class 'circular' using default value for the following components:
## type: 'angles'
## template: 'none'
## zero: 0
## rotation: 'counter'
## evalexprenvirenclos## Warning in watson.williams.test.default(x = angles_sub, group = groups_sub):
## Concentration parameters ( 389, 3335) not equal between groups. The test might
## not be applicable## Warning in watson.williams.test.default(x = angles_sub, group = groups_sub):
## Concentration parameters ( 105, 3335) not equal between groups. The test might
## not be applicable## Metric Comparison P_Value
## ewave_angle : baseline vs tac ewave_angle baseline vs tac 0.35306216
## ewave_angle : baseline vs detac ewave_angle baseline vs detac 0.92583343
## ewave_angle : tac vs detac ewave_angle tac vs detac 0.38313492
## cycle_angle : baseline vs tac cycle_angle baseline vs tac 0.35314393
## cycle_angle : baseline vs detac cycle_angle baseline vs detac 0.36531649
## cycle_angle : tac vs detac cycle_angle tac vs detac 0.08292290
## sys_im_angle : baseline vs tac sys_im_angle baseline vs tac 0.31379701
## sys_im_angle : baseline vs detac sys_im_angle baseline vs detac 0.18295082
## sys_im_angle : tac vs detac sys_im_angle tac vs detac 0.07615933
## Significant_Adj
## ewave_angle : baseline vs tac No
## ewave_angle : baseline vs detac No
## ewave_angle : tac vs detac No
## cycle_angle : baseline vs tac No
## cycle_angle : baseline vs detac No
## cycle_angle : tac vs detac No
## sys_im_angle : baseline vs tac No
## sys_im_angle : baseline vs detac No
## sys_im_angle : tac vs detac No## Warning: package 'MuMIn' was built under R version 4.4.3## Registered S3 methods overwritten by 'MuMIn':
## method from
## nobs.multinom broom
## nobs.fitdistr broom## boundary (singular) fit: see help('isSingular')
## boundary (singular) fit: see help('isSingular')
## boundary (singular) fit: see help('isSingular')