p-Nitrophenol Concentration from Cell Lysates
Plate reader absorbance measurements at 405 nm and calculated concentrations
Cell Lysate Plate Reader Absorbance
(First Reading)
Plate Reader Absorbance
(Second Reading)
Average Absorbance
Δ Absorbance
(Cell Lysate - Blank B)
p-NP Concentration
(µg/mL)
Group A
1 (pA1) 0.1820 0.4270 0.3045 0.1880 626.67
2 (pA2) 0.1860 0.1860 0.1860 0.0695 231.67
3 (pA3) 0.2350 0.1870 0.2110 0.0945 315.00
4 (pA4) 0.1310 0.8880 0.5095 0.3930 1,310.00
Group B
5 (pB1) 0.1810 0.1810 0.1810 0.0645 215.00
6 (pB2) 0.1930 0.1960 0.1945 0.0780 260.00
7 (pB3) 0.1870 0.1950 0.1910 0.0745 248.33
8 (pB4) 0.1860 0.2010 0.1935 0.0770 256.67
Group C
9 (pC1) 0.2190 0.2130 0.2160 0.0995 331.67
10 (pC2) 0.2130 0.2180 0.2155 0.0990 330.00
11 (pC3) 0.2000 0.2180 0.2090 0.0925 308.33
12 (pC4) 0.2040 0.2070 0.2055 0.0890 296.67