| p-Nitrophenol Concentration from Cell Lysates | |||||
| Plate reader absorbance measurements at 405 nm and calculated concentrations | |||||
| Cell Lysate | Plate Reader Absorbance (First Reading) |
Plate Reader Absorbance (Second Reading) |
Average Absorbance |
Δ Absorbance (Cell Lysate - Blank B) |
p-NP Concentration (µg/mL) |
|---|---|---|---|---|---|
| Group A | |||||
| 1 (pA1) | 0.1820 | 0.4270 | 0.3045 | 0.1880 | 626.67 |
| 2 (pA2) | 0.1860 | 0.1860 | 0.1860 | 0.0695 | 231.67 |
| 3 (pA3) | 0.2350 | 0.1870 | 0.2110 | 0.0945 | 315.00 |
| 4 (pA4) | 0.1310 | 0.8880 | 0.5095 | 0.3930 | 1,310.00 |
| Group B | |||||
| 5 (pB1) | 0.1810 | 0.1810 | 0.1810 | 0.0645 | 215.00 |
| 6 (pB2) | 0.1930 | 0.1960 | 0.1945 | 0.0780 | 260.00 |
| 7 (pB3) | 0.1870 | 0.1950 | 0.1910 | 0.0745 | 248.33 |
| 8 (pB4) | 0.1860 | 0.2010 | 0.1935 | 0.0770 | 256.67 |
| Group C | |||||
| 9 (pC1) | 0.2190 | 0.2130 | 0.2160 | 0.0995 | 331.67 |
| 10 (pC2) | 0.2130 | 0.2180 | 0.2155 | 0.0990 | 330.00 |
| 11 (pC3) | 0.2000 | 0.2180 | 0.2090 | 0.0925 | 308.33 |
| 12 (pC4) | 0.2040 | 0.2070 | 0.2055 | 0.0890 | 296.67 |