Overview
This notebook combines two reviewer-facing analyses across the same cohorts (TNBC/BRCA, PC, OV):
Part I — RNA (CCLE)
Extract BRCA1P1 expression (RPKM) from the CCLE RNA-seq GCT, map CCLE sample IDs to DepMap ModelIDs (ACH-…), and test whether BRCA1P1 expression differs by:
BRCA1 mutation status (WT vs Mut)
BRCA2 mutation status (WT vs Mut)
BRCA1/2 combined (WT vs Mut)
Part II — Copy number (DepMap WGS Segments)
Extract BRCA1P1 copy number from DepMap WGS segmentation calls (BRCA1P1 is a pseudogene; not present in gene-level CN matrices), and test whether BRCA1P1 CN differs by:
BRCA1 mutation status (WT vs Mut)
BRCA2 mutation status (WT vs Mut)
BRCA1/2 combined (WT vs Mut)
Outputs:
Per-cohort plots + summary tables
Per-cohort Excel workbooks
Combined Excel workbook across cohorts (RNA) and across cohorts (CNV)
Part I — RNA expression (CCLE BRCA1P1 RPKM)
## Unique ModelIDs with BRCA1P1 RNA: 1019
| Status | lower_whisker | q1 | median | q3 | upper_whisker | n_points |
|---|---|---|---|---|---|---|
| Mut | 0 | 0 | 0 | 0 | 0 | 7 |
| WT | 0 | 0 | 0 | 0 | 0 | 14 |
| Status | lower_whisker | q1 | median | q3 | upper_whisker | n_points |
|---|---|---|---|---|---|---|
| Mut | 0.095 | 0.128 | 0.161 | 0.194 | 0.227 | 2 |
| WT | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 6 |
| Status | lower_whisker | q1 | median | q3 | upper_whisker | n_points |
|---|---|---|---|---|---|---|
| Mut | 0 | 0 | 0 | 0 | 0 | 7 |
| WT | 0 | 0 | 0 | 0 | 0 | 40 |
Part II — Copy number (DepMap WGS segments BRCA1P1 CNV)
| Status | lower_whisker | q1 | median | q3 | upper_whisker | n_points |
|---|---|---|---|---|---|---|
| Mut | 0.726 | 0.847 | 0.886 | 1.078 | 1.424 | 7 |
| WT | 0.684 | 0.837 | 0.959 | 1.088 | 1.375 | 15 |
| Status | lower_whisker | q1 | median | q3 | upper_whisker | n_points |
|---|---|---|---|---|---|---|
| Mut | 1.012 | 1.019 | 1.027 | 1.075 | 1.122 | 3 |
| WT | 0.768 | 0.891 | 1.013 | 1.026 | 1.038 | 3 |
| Status | lower_whisker | q1 | median | q3 | upper_whisker | n_points |
|---|---|---|---|---|---|---|
| Mut | 0.718 | 0.867 | 0.934 | 1.041 | 1.053 | 5 |
| WT | 0.768 | 1.006 | 1.056 | 1.165 | 1.404 | 40 |