AI prompt battle
AI model
AI model used: microsoft copilot
Original text
The Microscopic Elegance of the DAPI Stain
DAPI (4′,6-diamidino-2-phenylindole) is a fluorescent stain that binds strongly to A-T rich regions in DNA. Because it can pass through an intact cell membrane, it is widely used in live and fixed cell microscopy for nuclear and chromosome counterstaining. Upon binding to DNA, DAPI exhibits a fluorescence emission maximum at approximately 461 nm, a characteristic blue glow when exposed to ultraviolet light. The stain is commonly used in flow cytometry and fluorescence microscopy to visualize the nucleus with exceptional detail, distinguishing it from other cellular components and enabling precise quantification of DNA content. Proper protocol requires a dilution of 1-300 nM, followed by a 5-10 minute incubation period in the dark to minimize photobleaching.
Iteration 1
Prompt
write an short text about dapi. Start with what abbreviation DAPI is and what it binds to
16 words
Output
DAPI stands for 4′,6-diamidino-2-phenylindole. It is a fluorescent stain commonly used in biological research to visualize DNA. DAPI binds strongly to adenine-thymine (A-T) rich regions in the minor groove of double-stranded DNA. When bound, it emits a bright blue fluorescence under ultraviolet light, making it ideal for staining cell nuclei in fluorescence microscopy.
Iteration 2
Prompt
with the title The Microscopic Elegance of the DAPI Stain
10 words
Output
The Microscopic Elegance of the DAPI Stain
DAPI, short for 4′,6-diamidino-2-phenylindole, is a widely used fluorescent dye in cell biology and microscopy. Its unique property lies in its strong affinity for adenine-thymine (A-T) rich regions of double-stranded DNA, where it binds within the minor groove. When excited by ultraviolet light, DAPI emits a brilliant blue fluorescence, making it an indispensable tool for visualizing cell nuclei. This elegant stain is commonly employed in applications such as cell cycle analysis, apoptosis detection, and immunofluorescence imaging, offering researchers a clear and vivid view of the genetic material within cells.
Iteration 3
Prompt
make it less detailed
4 words
Output
The Microscopic Elegance of the DAPI Stain
DAPI, short for 4′,6-diamidino-2-phenylindole, is a fluorescent dye that binds strongly to A-T rich regions of DNA. When exposed to ultraviolet light, it emits a bright blue glow, making it a popular choice for staining cell nuclei in microscopy.
Iteration 4
Prompt
the first sentence is good. In the second sentence describe that is can pass through an intact cell membrane and that this is used for live and fixed cell microscopy
30 words
Output
The Microscopic Elegance of the DAPI Stain
DAPI, short for 4′,6-diamidino-2-phenylindole, is a fluorescent dye that binds strongly to A-T rich regions of DNA. It can pass through an intact cell membrane, allowing its use in both live-cell and fixed-cell microscopy, where it emits a bright blue glow under ultraviolet light.
Iteration 5
Prompt
in the third sentence add what happend upon binding to DNA. add the maximum emmision and that is gives a characteristic blue glow
23 words
Output
The Microscopic Elegance of the DAPI Stain DAPI, short for 4′,6-diamidino-2-phenylindole, is a fluorescent dye that binds strongly to A-T rich regions of DNA. It can pass through an intact cell membrane, allowing its use in both live-cell and fixed-cell microscopy. Upon binding to DNA, DAPI’s fluorescence intensifies, reaching a maximum emission around 461 nm, which gives a characteristic bright blue glow under ultraviolet light.
Itiration 6
Prompt
this is good so far. after that add what the stain is most commonly used for. mention flow cytometry and then fluorescence microscopy
24 words
Output
The Microscopic Elegance of the DAPI Stain
DAPI, short for 4′,6-diamidino-2-phenylindole, is a fluorescent dye that binds strongly to A-T rich regions of DNA. It can pass through an intact cell membrane, allowing its use in both live-cell and fixed-cell microscopy. Upon binding to DNA, DAPI’s fluorescence intensifies, reaching a maximum emission around 461 nm, which gives a characteristic bright blue glow under ultraviolet light. This stain is most commonly used for nuclear staining in fluorescence microscopy and is also widely applied in flow cytometry for DNA content analysis.
Itiration 7
Prompt
reverse the order of fluorescence microscopy and flow cytometry. Add that it can visualize the nucleus with exceptional detail
25 words
Output
The Microscopic Elegance of the DAPI Stain
DAPI, short for 4′,6-diamidino-2-phenylindole, is a fluorescent dye that binds strongly to A-T rich regions of DNA. It can pass through an intact cell membrane, allowing its use in both live-cell and fixed-cell microscopy. Upon binding to DNA, DAPI’s fluorescence intensifies, reaching a maximum emission around 461 nm, which gives a characteristic bright blue glow under ultraviolet light. This stain is most commonly used in flow cytometry for DNA content analysis and in fluorescence microscopy, where it visualizes the nucleus with exceptional detail.
Itiration 8
Prompt
add that you can distinguish the nucleus from other celllar components
11 words
Output
The Microscopic Elegance of the DAPI Stain
DAPI, short for 4′,6-diamidino-2-phenylindole, is a fluorescent dye that binds strongly to A-T rich regions of DNA. It can pass through an intact cell membrane, allowing its use in both live-cell and fixed-cell microscopy. Upon binding to DNA, DAPI’s fluorescence intensifies, reaching a maximum emission around 461 nm, which gives a characteristic bright blue glow under ultraviolet light. This stain is most commonly used in flow cytometry for DNA content analysis and in fluorescence microscopy, where it visualizes the nucleus with exceptional detail and allows researchers to clearly distinguish it from other cellular components.
Itiration 9
Prompt
this is good so far. Now add what dilution and incubation period should be used in proper protocol
18 words
Output
The Microscopic Elegance of the DAPI Stain DAPI, short for 4′,6-diamidino-2‑phenylindole, is a fluorescent dye that binds strongly to A-T rich regions of DNA. It can pass through an intact cell membrane, allowing its use in both live‑cell and fixed‑cell microscopy. Upon binding to DNA, DAPI’s fluorescence intensifies, reaching a maximum emission around 461 nm, which gives a characteristic bright blue glow under ultraviolet light. This stain is most commonly used in flow cytometry for DNA content analysis and in fluorescence microscopy, where it visualizes the nucleus with exceptional detail and allows researchers to clearly distinguish it from other cellular components. Recommended dilution & incubation protocol:
Fixed cells (fluorescence microscopy): Prepare a working solution at 300 nM (≈0.1 µg/mL), incubate cells for 1–5 minutes at room temperature, protected from light, then wash and image. [thermofisher.com], [creative-b…oarray.com] Live cells (flow cytometry): Use DAPI at 0.1 µg/mL or 0.1 µg/mL–1 µg/mL (≈0.3–3 µM), incubate for 10–15 minutes at 37 °C (microscopy) or 15 minutes at room temperature (flow cytometry), then proceed with analysis.
Itiration 10
Prompt
write it as a continuation on the rest of the text not point by point
15 words
Output
The Microscopic Elegance of the DAPI Stain
DAPI, short for 4′,6-diamidino-2‑phenylindole, is a fluorescent dye that binds strongly to A-T rich regions of DNA. It can pass through an intact cell membrane, allowing its use in both live‑cell and fixed‑cell microscopy. Upon binding to DNA, DAPI’s fluorescence intensifies, reaching a maximum emission around 461 nm, which gives a characteristic bright blue glow under ultraviolet light. This stain is most commonly used in flow cytometry for DNA content analysis and in fluorescence microscopy, where it visualizes the nucleus with exceptional detail and allows researchers to clearly distinguish it from other cellular components. In proper protocols, DAPI is typically used at a dilution of about 0.1 µg/mL (≈300 nM) for fixed cells, with an incubation period of 1–5 minutes at room temperature, protected from light. —