Time (Years)

Physical Changes

Chemical Changes

Biological / Other Effects

Impact on Contaminants

Key Controlling Factors

2

Retains original structure and porosity; labile components react with water and oxygen.

Formation of initial hydroxyl and carboxyl functional groups; pH increase due to alkalinity.

Early microbial colonization; slight modification by root exudates.

Immobilization of heavy metals and organics through adsorption, cation exchange, surface complexation.

Abiotic: moisture, temperature, oxygen; Biotic: microbial colonization, root exudates; Biochar properties: feedstock, porosity, alkalinity.

5

Partial collapse of pores; formation of micro-aggregates; colloidal/nanoscale particle formation begins.

Increase in oxygen-containing functional groups (OFGs), cation exchange capacity (CEC) and polarity; partial reduction in aromaticity.

Biochar aggregates with soil; microbial activity enhances functionalization; light-driven radical formation begins.

Stabilization of metals in aggregates; some metals may start mobilizing due to organic acids.

Abiotic: wet-dry and freeze-thaw cycles, light exposure; Biotic: microbial and root activity; Biochar: surface area, aromaticity, CEC.

20

Significant surface oxidation; collapse of porous structure; colloidal/nanoscale particle detachment and movement.

Advanced oxidation; carboxyl-rich functional groups; persistent free radicals; partial carbon loss.

Biochar fully integrates into soil aggregates; ongoing microbial and root interactions.

Mixed effects: continued immobilization via aggregates and OFGs; partial remobilization from pH decline, structural degradation, and colloid transport.

Abiotic: long-term oxidation, acid rain, UV, moisture fluctuations; Biotic: ongoing microbial and root processes; Biochar: stability, initial porosity, chemical composition.