Position	Substitution	Gene	Protein Function	Effect	Impact	Protein Change	Interpretation
782745	C→A	flgB	Flagellar basal body rod protein	Stop gained	High	p.Ser132* (132/148)	Truncation likely leads to loss of function
2517992	A→T	rsxD	Ion-translocating oxidoreductase complex subunit D	Stop gained	High	p.Cys265* (265/364)	Premature stop likely disrupts electron transport/redox role
469100	T→C	ettA	Energy-dependent translational throttle protein EttA	Stop lost	High	p.Ter211Trpext*? (211/553)	Protein extension may result in abnormal or nonfunctional product