O.Lurida Wildtype vs. Hatchery
Karina
2025-06-04
#Project:Wildtype vs. Hatchery O.Lurida genetic comparison
Olympia oyster
The goal of this project is to see if there are genetic differences between olympia oysters from a hatchery group and a wild type group. This could be useful for understanding if hatchery bred oyster can be used to restore wild type populations Data was originally collected by PSRF
#Workflow I received the data as BAM files and uploaded into Rstudio wild type samples
wget --recursive --no-parent --no-directories \
--no-check-certificate \
--accept=CSMB17W*.bam \
https://gannet.fish.washington.edu/acropora/OlyRAD_6plates/CSMB17W.v8/and for hatchery samples
wget --recursive --no-parent --no-directories \
--no-check-certificate \
--accept=CSMB18H*.bam \
https://gannet.fish.washington.edu/acropora/OlyRAD_6plates-v2/ #Merged two groups to make comparison Call variants for hatchery (summary of read alignments against the reference) These files contain the variants called from the respective BAM files aligned to the reference
for i in {01..05}; do
/home/shared/bcftools-1.14/bcftools mpileup -Ou -f \
/home/shared/8TB_HDD_02/thielkla/Karina-chinook/Olurida_v081.fa \
/home/shared/8TB_HDD_02/thielkla/Karina-chinook/CSBM18H/CSMB18H.${i}.bam \
| /home/shared/bcftools-1.14/bcftools call -mv -Ov \
-o ~/Karina-chinook/output/H${i}.vcf
done
Call variants for wild type
for i in {01..05}; do
/home/shared/bcftools-1.14/bcftools mpileup -Ou -f \
/home/shared/8TB_HDD_02/thielkla/Karina-chinook/Olurida_v081.fa \
/home/shared/8TB_HDD_02/thielkla/Karina-chinook/CSMB17W/CSMB17W.${i}.bam \
| /home/shared/bcftools-1.14/bcftools call -mv -Ov \
-o ~/Karina-chinook/output/W${i}.vcf
doneMerged wild type and hatchery for comparison (This prepares your VCF files for efficient querying, visualization, and further analysis)
for f in ~/Karina-chinook/output/*.vcf; do
/home/shared/htslib-1.14/bgzip "$f" # compress to .vcf.gz
/home/shared/htslib-1.14/tabix -p vcf "$f.gz" # index for random access
donels ~/Karina-chinook/output/*.vcf.gz > ~/Karina-chinook/output/vcflist.txtoutput:merged.vcf.gz — compressed multi-sample VCF output:merged.vcf.gz.csi — index file for that VCF
/home/shared/bcftools-1.14/bcftools merge \
-l ~/Karina-chinook/output/vcflist.txt -Oz -o ~/Karina-chinook/output/merged.vcf.gz
/home/shared/bcftools-1.14/bcftools index ~/Karina-chinook/output/merged.vcf.gzThese are now ready for downstream analysis
/home/shared/bcftools-1.14/bcftools merge \
-l ~/Karina-chinook/output/vcflist.txt -Oz -o ~/Karina-chinook/output/merged.vcf.gz
/home/shared/bcftools-1.14/bcftools index ~/Karina-chinook/output/merged.vcf.gz#Compare FST Create text file that list samples
/home/shared/vcftools-0.1.16/bin/vcftools --gzvcf ~/Karina-chinook/output/merged.vcf.gz \
--weir-fst-pop hatchery.txt \
--weir-fst-pop wild.txt \
--out ~/Karina-chinook/output/hatchery_vs_wild#Compare FST To measure the degree of genetic differentiation between populations
head -20 ~/Karina-chinook/output/hatchery_vs_wild.weir.fst #Table Create a data frame called hatchery_vs_wild.weir.fst Make table from fst data
# Install if not already installed
#install.packages("DT")
# Load the package
library(DT)
# Read your CSV file
hatchery_vs_wild.weir.fst <- read.csv("https://gannet.fish.washington.edu/seashell/snaps/Top_1__FST_Loci.csv")
# Display interactive table
datatable(hatchery_vs_wild.weir.fst, options = list(pageLength = 10, scrollX = TRUE), rownames = FALSE)#Make Bedgraph from top SNPS Top genetic variations between groups
# Load necessary library
library(readr)
# Read CSV (assuming your file is called "input.csv")
df <- read_csv("https://gannet.fish.washington.edu/seashell/snaps/Top_1__FST_Loci.csv", col_types = cols())
# Drop the first column
df <- df[, -1]
# Rename columns for clarity
colnames(df) <- c("chrom", "pos", "score")
# Convert to bedGraph format: chrom, start (0-based), end (1-based), score
df$start <- df$pos - 1
df$end <- df$start + 1
# Reorder columns
bedgraph <- df[, c("chrom", "start", "end", "score")]
# Write to tab-separated file with no header
write.table(bedgraph, file = "/home/shared/8TB_HDD_02/thielkla/Karina-chinook/Karina-chinook-v2/project/TopSNP.bedgraph", sep = "\t", quote = FALSE, row.names = FALSE, col.names = FALSE)#Closest SNPs and genes Identify genes that are physically closest to a set of SNPs using a gene annotation file and a list of top SNPs
curl -O https://owl.fish.washington.edu/halfshell/genomic-databank/Olurida_v081-20190709.gene.gffSorts the GFF file by chromosome (column 1) and by start position (column 4).
sort -k1,1 -k4,4n Olurida_v081-20190709.gene.gff > Olurida_v081-20190709.gene.sorted.gffSort the SNP file
sort -k1,1 -k2,2n /home/shared/8TB_HDD_02/thielkla/Karina-chinook/Karina-chinook-v2/project/TopSNP.bedgraph > /home/shared/8TB_HDD_02/thielkla/Karina-chinook/Karina-chinook-v2/project/TopSNP.sorted.bedgraph#Find closest gene to each SNP Each line in the output corresponds to a SNP, followed by the closest gene’s information
/home/shared/bedtools2/bin/bedtools closest \
-a /home/shared/8TB_HDD_02/thielkla/Karina-chinook/Karina-chinook-v2/project/TopSNP.sorted.bedgraph \
-b Olurida_v081-20190709.gene.sorted.gff \
> /home/shared/8TB_HDD_02/thielkla/Karina-chinook-v2/project/09-snp-gene-closet.out
head /home/shared/8TB_HDD_02/thielkla/Karina-chinook-v2/project/09-snp-gene-closet.out## Contig100908 451 452 1 . . . -1 -1 . . . .
## Contig103716 1665 1666 0.666667 . . . -1 -1 . . . .
## Contig104631 524 525 0.714286 . . . -1 -1 . . . .
## Contig105717 3961 3962 0.666667 . . . -1 -1 . . . .
## Contig105736 902 903 1 Contig105736 maker gene 704 1576 . + . ID=OLUR_00030760;Name=OLUR_00030760;Alias=maker-Contig105736-snap-gene-0.1;Note=Similar to PI4KA: Phosphatidylinositol 4-kinase alpha (Bos taurus OX%3D9913);
## Contig106498 8274 8275 1 Contig106498 maker gene 5400 7804 . + . ID=OLUR_00012823;Name=OLUR_00012823;Alias=maker-Contig106498-snap-gene-0.3;Note=Protein of unknown function;Dbxref=MobiDBLite:mobidb-lite;
## Contig1083 37671 37672 0.666667 Contig1083 maker gene 5035 37419 . + . ID=OLUR_00003165;Name=OLUR_00003165;Alias=snap_masked-Contig1083-processed-gene-0.0;Note=Similar to USP7: Ubiquitin carboxyl-terminal hydrolase 7 (Gallus gallus OX%3D9031);Dbxref=Gene3D:G3DSA:2.60.210.10,Gene3D:G3DSA:3.90.70.10,InterPro:IPR001394,InterPro:IPR008974,InterPro:IPR018200,InterPro:IPR028889,InterPro:IPR038765,Pfam:PF00443,ProSitePatterns:PS00972,ProSiteProfiles:PS50235,SUPERFAMILY:SSF49599,SUPERFAMILY:SSF54001;Ontology_term=GO:0005515,GO:0006511,GO:0016579,GO:0036459;
## Contig1083 37725 37726 0.666667 Contig1083 maker gene 5035 37419 . + . ID=OLUR_00003165;Name=OLUR_00003165;Alias=snap_masked-Contig1083-processed-gene-0.0;Note=Similar to USP7: Ubiquitin carboxyl-terminal hydrolase 7 (Gallus gallus OX%3D9031);Dbxref=Gene3D:G3DSA:2.60.210.10,Gene3D:G3DSA:3.90.70.10,InterPro:IPR001394,InterPro:IPR008974,InterPro:IPR018200,InterPro:IPR028889,InterPro:IPR038765,Pfam:PF00443,ProSitePatterns:PS00972,ProSiteProfiles:PS50235,SUPERFAMILY:SSF49599,SUPERFAMILY:SSF54001;Ontology_term=GO:0005515,GO:0006511,GO:0016579,GO:0036459;
## Contig113550 4322 4323 1 . . . -1 -1 . . . .
## Contig117009 829 830 1 . . . -1 -1 . . . .#Table of closest genes and function
grep -oP 'Note=Similar to \K[^(;)]+' /home/shared/8TB_HDD_02/thielkla/Karina-chinook/Karina-chinook-v2/project/09-snp-gene-closet.out | sort | uniq
cat /home/shared/8TB_HDD_02/thielkla/Karina-chinook-v2/project/09-snp-gene-closet.out## Contig100908 451 452 1 . . . -1 -1 . . . .
## Contig103716 1665 1666 0.666667 . . . -1 -1 . . . .
## Contig104631 524 525 0.714286 . . . -1 -1 . . . .
## Contig105717 3961 3962 0.666667 . . . -1 -1 . . . .
## Contig105736 902 903 1 Contig105736 maker gene 704 1576 . + . ID=OLUR_00030760;Name=OLUR_00030760;Alias=maker-Contig105736-snap-gene-0.1;Note=Similar to PI4KA: Phosphatidylinositol 4-kinase alpha (Bos taurus OX%3D9913);
## Contig106498 8274 8275 1 Contig106498 maker gene 5400 7804 . + . ID=OLUR_00012823;Name=OLUR_00012823;Alias=maker-Contig106498-snap-gene-0.3;Note=Protein of unknown function;Dbxref=MobiDBLite:mobidb-lite;
## Contig1083 37671 37672 0.666667 Contig1083 maker gene 5035 37419 . + . ID=OLUR_00003165;Name=OLUR_00003165;Alias=snap_masked-Contig1083-processed-gene-0.0;Note=Similar to USP7: Ubiquitin carboxyl-terminal hydrolase 7 (Gallus gallus OX%3D9031);Dbxref=Gene3D:G3DSA:2.60.210.10,Gene3D:G3DSA:3.90.70.10,InterPro:IPR001394,InterPro:IPR008974,InterPro:IPR018200,InterPro:IPR028889,InterPro:IPR038765,Pfam:PF00443,ProSitePatterns:PS00972,ProSiteProfiles:PS50235,SUPERFAMILY:SSF49599,SUPERFAMILY:SSF54001;Ontology_term=GO:0005515,GO:0006511,GO:0016579,GO:0036459;
## Contig1083 37725 37726 0.666667 Contig1083 maker gene 5035 37419 . + . ID=OLUR_00003165;Name=OLUR_00003165;Alias=snap_masked-Contig1083-processed-gene-0.0;Note=Similar to USP7: Ubiquitin carboxyl-terminal hydrolase 7 (Gallus gallus OX%3D9031);Dbxref=Gene3D:G3DSA:2.60.210.10,Gene3D:G3DSA:3.90.70.10,InterPro:IPR001394,InterPro:IPR008974,InterPro:IPR018200,InterPro:IPR028889,InterPro:IPR038765,Pfam:PF00443,ProSitePatterns:PS00972,ProSiteProfiles:PS50235,SUPERFAMILY:SSF49599,SUPERFAMILY:SSF54001;Ontology_term=GO:0005515,GO:0006511,GO:0016579,GO:0036459;
## Contig113550 4322 4323 1 . . . -1 -1 . . . .
## Contig117009 829 830 1 . . . -1 -1 . . . .
## Contig120604 1 2 1 . . . -1 -1 . . . .
## Contig1225 15492 15493 0.666667 Contig1225 maker gene 19872 23256 . + . ID=OLUR_00004191;Name=OLUR_00004191;Alias=snap_masked-Contig1225-processed-gene-0.1;Note=Protein of unknown function;Dbxref=InterPro:IPR004875,Pfam:PF03184;Ontology_term=GO:0003676;
## Contig1225 15507 15508 0.666667 Contig1225 maker gene 19872 23256 . + . ID=OLUR_00004191;Name=OLUR_00004191;Alias=snap_masked-Contig1225-processed-gene-0.1;Note=Protein of unknown function;Dbxref=InterPro:IPR004875,Pfam:PF03184;Ontology_term=GO:0003676;
## Contig128671 625 626 0.714286 . . . -1 -1 . . . .
## Contig130167 1437 1438 1 . . . -1 -1 . . . .
## Contig1308 16492 16493 0.666667 Contig1308 maker gene 5099 22704 . + . ID=OLUR_00003680;Name=OLUR_00003680;Alias=maker-Contig1308-snap-gene-0.6;Note=Protein of unknown function;Dbxref=MobiDBLite:mobidb-lite;
## Contig134953 2529 2530 0.714286 . . . -1 -1 . . . .
## Contig135690 2185 2186 0.666667 . . . -1 -1 . . . .
## Contig135690 2187 2188 0.666667 . . . -1 -1 . . . .
## Contig145166 1586 1587 0.666667 . . . -1 -1 . . . .
## Contig145203 1321 1322 1 . . . -1 -1 . . . .
## Contig147622 2 3 1 . . . -1 -1 . . . .
## Contig150554 6248 6249 0.818182 Contig150554 maker gene 2577 6235 . + . ID=OLUR_00026108;Name=OLUR_00026108;Alias=maker-Contig150554-snap-gene-0.1;Note=Protein of unknown function;Dbxref=Gene3D:G3DSA:3.10.20.90,InterPro:IPR011009,SUPERFAMILY:SSF56112;
## Contig150742 1459 1460 0.666667 . . . -1 -1 . . . .
## Contig154616 1109 1110 1 . . . -1 -1 . . . .
## Contig167150 1437 1438 1 . . . -1 -1 . . . .
## Contig167362 537 538 0.714286 . . . -1 -1 . . . .
## Contig16834 407 408 0.666667 Contig16834 maker gene 1199 7618 . - . ID=OLUR_00017393;Name=OLUR_00017393;Alias=maker-Contig16834-snap-gene-0.1;Note=Similar to CYP10: Cytochrome P450 10 (Lymnaea stagnalis OX%3D6523);Dbxref=Gene3D:G3DSA:1.10.630.10,InterPro:IPR001128,InterPro:IPR002401,InterPro:IPR017972,InterPro:IPR036396,PRINTS:PR00385,PRINTS:PR00463,Pfam:PF00067,ProSitePatterns:PS00086,SUPERFAMILY:SSF48264;Ontology_term=GO:0005506,GO:0016705,GO:0020037,GO:0055114;
## Contig16834 409 410 0.666667 Contig16834 maker gene 1199 7618 . - . ID=OLUR_00017393;Name=OLUR_00017393;Alias=maker-Contig16834-snap-gene-0.1;Note=Similar to CYP10: Cytochrome P450 10 (Lymnaea stagnalis OX%3D6523);Dbxref=Gene3D:G3DSA:1.10.630.10,InterPro:IPR001128,InterPro:IPR002401,InterPro:IPR017972,InterPro:IPR036396,PRINTS:PR00385,PRINTS:PR00463,Pfam:PF00067,ProSitePatterns:PS00086,SUPERFAMILY:SSF48264;Ontology_term=GO:0005506,GO:0016705,GO:0020037,GO:0055114;
## Contig17444 18301 18302 1 . . . -1 -1 . . . .
## Contig175710 2602 2603 1 . . . -1 -1 . . . .
## Contig17603 60217 60218 0.714286 Contig17603 maker gene 57580 67665 . + . ID=OLUR_00000590;Name=OLUR_00000590;Alias=maker-Contig17603-snap-gene-0.6;Note=Protein of unknown function;Dbxref=Coils:Coil,MobiDBLite:mobidb-lite;
## Contig17603 60308 60309 0.714286 Contig17603 maker gene 57580 67665 . + . ID=OLUR_00000590;Name=OLUR_00000590;Alias=maker-Contig17603-snap-gene-0.6;Note=Protein of unknown function;Dbxref=Coils:Coil,MobiDBLite:mobidb-lite;
## Contig17603 60314 60315 0.714286 Contig17603 maker gene 57580 67665 . + . ID=OLUR_00000590;Name=OLUR_00000590;Alias=maker-Contig17603-snap-gene-0.6;Note=Protein of unknown function;Dbxref=Coils:Coil,MobiDBLite:mobidb-lite;
## Contig17603 60315 60316 0.714286 Contig17603 maker gene 57580 67665 . + . ID=OLUR_00000590;Name=OLUR_00000590;Alias=maker-Contig17603-snap-gene-0.6;Note=Protein of unknown function;Dbxref=Coils:Coil,MobiDBLite:mobidb-lite;
## Contig17603 60374 60375 0.714286 Contig17603 maker gene 57580 67665 . + . ID=OLUR_00000590;Name=OLUR_00000590;Alias=maker-Contig17603-snap-gene-0.6;Note=Protein of unknown function;Dbxref=Coils:Coil,MobiDBLite:mobidb-lite;
## Contig17603 60443 60444 0.714286 Contig17603 maker gene 57580 67665 . + . ID=OLUR_00000590;Name=OLUR_00000590;Alias=maker-Contig17603-snap-gene-0.6;Note=Protein of unknown function;Dbxref=Coils:Coil,MobiDBLite:mobidb-lite;
## Contig17603 60445 60446 0.714286 Contig17603 maker gene 57580 67665 . + . ID=OLUR_00000590;Name=OLUR_00000590;Alias=maker-Contig17603-snap-gene-0.6;Note=Protein of unknown function;Dbxref=Coils:Coil,MobiDBLite:mobidb-lite;
## Contig17603 60454 60455 0.714286 Contig17603 maker gene 57580 67665 . + . ID=OLUR_00000590;Name=OLUR_00000590;Alias=maker-Contig17603-snap-gene-0.6;Note=Protein of unknown function;Dbxref=Coils:Coil,MobiDBLite:mobidb-lite;
## Contig17603 60468 60469 0.714286 Contig17603 maker gene 57580 67665 . + . ID=OLUR_00000590;Name=OLUR_00000590;Alias=maker-Contig17603-snap-gene-0.6;Note=Protein of unknown function;Dbxref=Coils:Coil,MobiDBLite:mobidb-lite;
## Contig17603 60595 60596 0.714286 Contig17603 maker gene 57580 67665 . + . ID=OLUR_00000590;Name=OLUR_00000590;Alias=maker-Contig17603-snap-gene-0.6;Note=Protein of unknown function;Dbxref=Coils:Coil,MobiDBLite:mobidb-lite;
## Contig17603 60596 60597 0.714286 Contig17603 maker gene 57580 67665 . + . ID=OLUR_00000590;Name=OLUR_00000590;Alias=maker-Contig17603-snap-gene-0.6;Note=Protein of unknown function;Dbxref=Coils:Coil,MobiDBLite:mobidb-lite;
## Contig17603 60600 60601 0.714286 Contig17603 maker gene 57580 67665 . + . ID=OLUR_00000590;Name=OLUR_00000590;Alias=maker-Contig17603-snap-gene-0.6;Note=Protein of unknown function;Dbxref=Coils:Coil,MobiDBLite:mobidb-lite;
## Contig17603 60643 60644 0.714286 Contig17603 maker gene 57580 67665 . + . ID=OLUR_00000590;Name=OLUR_00000590;Alias=maker-Contig17603-snap-gene-0.6;Note=Protein of unknown function;Dbxref=Coils:Coil,MobiDBLite:mobidb-lite;
## Contig17603 60687 60688 0.714286 Contig17603 maker gene 57580 67665 . + . ID=OLUR_00000590;Name=OLUR_00000590;Alias=maker-Contig17603-snap-gene-0.6;Note=Protein of unknown function;Dbxref=Coils:Coil,MobiDBLite:mobidb-lite;
## Contig17603 60724 60725 0.714286 Contig17603 maker gene 57580 67665 . + . ID=OLUR_00000590;Name=OLUR_00000590;Alias=maker-Contig17603-snap-gene-0.6;Note=Protein of unknown function;Dbxref=Coils:Coil,MobiDBLite:mobidb-lite;
## Contig17603 60744 60745 0.714286 Contig17603 maker gene 57580 67665 . + . ID=OLUR_00000590;Name=OLUR_00000590;Alias=maker-Contig17603-snap-gene-0.6;Note=Protein of unknown function;Dbxref=Coils:Coil,MobiDBLite:mobidb-lite;
## Contig17603 60759 60760 0.714286 Contig17603 maker gene 57580 67665 . + . ID=OLUR_00000590;Name=OLUR_00000590;Alias=maker-Contig17603-snap-gene-0.6;Note=Protein of unknown function;Dbxref=Coils:Coil,MobiDBLite:mobidb-lite;
## Contig17603 60803 60804 0.714286 Contig17603 maker gene 57580 67665 . + . ID=OLUR_00000590;Name=OLUR_00000590;Alias=maker-Contig17603-snap-gene-0.6;Note=Protein of unknown function;Dbxref=Coils:Coil,MobiDBLite:mobidb-lite;
## Contig17603 60804 60805 0.714286 Contig17603 maker gene 57580 67665 . + . ID=OLUR_00000590;Name=OLUR_00000590;Alias=maker-Contig17603-snap-gene-0.6;Note=Protein of unknown function;Dbxref=Coils:Coil,MobiDBLite:mobidb-lite;
## Contig17603 60849 60850 0.714286 Contig17603 maker gene 57580 67665 . + . ID=OLUR_00000590;Name=OLUR_00000590;Alias=maker-Contig17603-snap-gene-0.6;Note=Protein of unknown function;Dbxref=Coils:Coil,MobiDBLite:mobidb-lite;
## Contig17603 60868 60869 0.714286 Contig17603 maker gene 57580 67665 . + . ID=OLUR_00000590;Name=OLUR_00000590;Alias=maker-Contig17603-snap-gene-0.6;Note=Protein of unknown function;Dbxref=Coils:Coil,MobiDBLite:mobidb-lite;
## Contig17603 60877 60878 0.714286 Contig17603 maker gene 57580 67665 . + . ID=OLUR_00000590;Name=OLUR_00000590;Alias=maker-Contig17603-snap-gene-0.6;Note=Protein of unknown function;Dbxref=Coils:Coil,MobiDBLite:mobidb-lite;
## Contig17603 60882 60883 0.714286 Contig17603 maker gene 57580 67665 . + . ID=OLUR_00000590;Name=OLUR_00000590;Alias=maker-Contig17603-snap-gene-0.6;Note=Protein of unknown function;Dbxref=Coils:Coil,MobiDBLite:mobidb-lite;
## Contig17603 60897 60898 0.714286 Contig17603 maker gene 57580 67665 . + . ID=OLUR_00000590;Name=OLUR_00000590;Alias=maker-Contig17603-snap-gene-0.6;Note=Protein of unknown function;Dbxref=Coils:Coil,MobiDBLite:mobidb-lite;
## Contig17635 6512 6513 1 Contig17635 maker gene 4117 9859 . - . ID=OLUR_00014477;Name=OLUR_00014477;Alias=maker-Contig17635-snap-gene-0.4;Note=Protein of unknown function;Dbxref=MobiDBLite:mobidb-lite;
## Contig178821 1194 1195 0.692308 . . . -1 -1 . . . .
## Contig18358 17094 17095 1 . . . -1 -1 . . . .
## Contig18367 36168 36169 0.714286 Contig18367 maker gene 7924 39772 . - . ID=OLUR_00002366;Name=OLUR_00002366;Alias=maker-Contig18367-snap-gene-0.6;Note=Similar to ifi30: Gamma-interferon-inducible lysosomal thiol reductase (Danio rerio OX%3D7955);Dbxref=InterPro:IPR004911,Pfam:PF03227;
## Contig18493 27850 27851 0.666667 Contig18493 maker gene 4286 29502 . + . ID=OLUR_00003807;Name=OLUR_00003807;Alias=maker-Contig18493-snap-gene-0.5;Note=Similar to CEP192: Centrosomal protein of 192 kDa (Homo sapiens OX%3D9606);Dbxref=Coils:Coil,Gene3D:G3DSA:2.60.40.10,InterPro:IPR013783,MobiDBLite:mobidb-lite;
## Contig18493 27867 27868 0.666667 Contig18493 maker gene 4286 29502 . + . ID=OLUR_00003807;Name=OLUR_00003807;Alias=maker-Contig18493-snap-gene-0.5;Note=Similar to CEP192: Centrosomal protein of 192 kDa (Homo sapiens OX%3D9606);Dbxref=Coils:Coil,Gene3D:G3DSA:2.60.40.10,InterPro:IPR013783,MobiDBLite:mobidb-lite;
## Contig18493 27875 27876 0.666667 Contig18493 maker gene 4286 29502 . + . ID=OLUR_00003807;Name=OLUR_00003807;Alias=maker-Contig18493-snap-gene-0.5;Note=Similar to CEP192: Centrosomal protein of 192 kDa (Homo sapiens OX%3D9606);Dbxref=Coils:Coil,Gene3D:G3DSA:2.60.40.10,InterPro:IPR013783,MobiDBLite:mobidb-lite;
## Contig18902 11014 11015 0.666667 Contig18902 maker gene 5818 15950 . + . ID=OLUR_00001986;Name=OLUR_00001986;Alias=maker-Contig18902-snap-gene-0.5;Note=Protein of unknown function;Dbxref=Gene3D:G3DSA:1.10.238.10,InterPro:IPR002048,InterPro:IPR011992,InterPro:IPR018247,MobiDBLite:mobidb-lite,ProSitePatterns:PS00018,ProSiteProfiles:PS50222,SUPERFAMILY:SSF47473;Ontology_term=GO:0005509;
## Contig18902 11014 11015 0.666667 Contig18902 maker gene 10094 33851 . - . ID=OLUR_00001987;Name=OLUR_00001987;Alias=maker-Contig18902-snap-gene-0.6;Note=Similar to CFAP65: Cilia- and flagella-associated protein 65 (Gallus gallus OX%3D9031);Dbxref=Coils:Coil,Gene3D:G3DSA:2.60.40.10,InterPro:IPR008962,InterPro:IPR013783,SUPERFAMILY:SSF49354;
## Contig18981 2524 2525 0.818182 Contig18981 maker gene 3893 8516 . - . ID=OLUR_00022462;Name=OLUR_00022462;Alias=maker-Contig18981-snap-gene-0.2;Note=Similar to Trpc4: Short transient receptor potential channel 4 (Mus musculus OX%3D10090);Dbxref=Coils:Coil,InterPro:IPR002153,MobiDBLite:mobidb-lite,PRINTS:PR01097;Ontology_term=GO:0005262,GO:0016020,GO:0070588;
## Contig19582 48524 48525 0.666667 Contig19582 maker gene 272 29681 . - . ID=OLUR_00001635;Name=OLUR_00001635;Alias=maker-Contig19582-snap-gene-0.5;Note=Similar to R3HDM2: R3H domain-containing protein 2 (Bos taurus OX%3D9913);Dbxref=CDD:cd02642,Coils:Coil,Gene3D:G3DSA:3.30.1370.50,InterPro:IPR001374,InterPro:IPR024771,InterPro:IPR036867,MobiDBLite:mobidb-lite,Pfam:PF01424,ProSiteProfiles:PS51061,ProSiteProfiles:PS51673,SMART:SM00393,SUPERFAMILY:SSF82708;Ontology_term=GO:0003676;
## Contig21040 6098 6099 0.666667 Contig21040 maker gene 248 15815 . - . ID=OLUR_00011223;Name=OLUR_00011223;Alias=maker-Contig21040-snap-gene-0.2;Note=Similar to mnb: Serine/threonine-protein kinase minibrain (Drosophila melanogaster OX%3D7227);
## Contig21534 1273 1274 0.666667 Contig21534 maker gene 2294 9918 . - . ID=OLUR_00019999;Name=OLUR_00019999;Alias=maker-Contig21534-snap-gene-0.2;Note=Protein of unknown function;Dbxref=CDD:cd01671,Coils:Coil,Gene3D:G3DSA:1.10.533.10,InterPro:IPR001315,InterPro:IPR011029,MobiDBLite:mobidb-lite,Pfam:PF00619,ProSiteProfiles:PS50209,SUPERFAMILY:SSF47986;Ontology_term=GO:0042981;
## Contig215746 1048 1049 1 . . . -1 -1 . . . .
## Contig21581 43142 43143 0.666667 Contig21581 maker gene 35305 55013 . - . ID=OLUR_00000338;Name=OLUR_00000338;Alias=snap_masked-Contig21581-processed-gene-0.8;Note=Similar to ADGRL3: Adhesion G protein-coupled receptor L3 (Homo sapiens OX%3D9606);Dbxref=CDD:cd15039,Gene3D:G3DSA:1.20.1070.10,InterPro:IPR000832,InterPro:IPR017981,Pfam:PF00002,ProSiteProfiles:PS50261;Ontology_term=GO:0004888,GO:0004930,GO:0007166,GO:0007186,GO:0016021;
## Contig21816 45874 45875 0.666667 Contig21816 maker gene 42557 58389 . - . ID=OLUR_00000421;Name=OLUR_00000421;Alias=maker-Contig21816-snap-gene-0.15;Note=Protein of unknown function;Dbxref=MobiDBLite:mobidb-lite;
## Contig21828 35404 35405 0.666667 Contig21828 maker gene 4360 37027 . + . ID=OLUR_00000676;Name=OLUR_00000676;Alias=maker-Contig21828-snap-gene-0.5;Note=Similar to FAM49B: Protein FAM49B (Homo sapiens OX%3D9606);Dbxref=InterPro:IPR009828,Pfam:PF07159;
## Contig21945 3341 3342 0.666667 Contig21945 maker gene 609 7174 . - . ID=OLUR_00024654;Name=OLUR_00024654;Alias=maker-Contig21945-snap-gene-0.1;Note=Protein of unknown function;
## Contig21996 11363 11364 0.666667 Contig21996 maker gene 40640 47940 . - . ID=OLUR_00000754;Name=OLUR_00000754;Alias=maker-Contig21996-snap-gene-0.4;Note=Similar to rost: Protein rolling stone (Drosophila melanogaster OX%3D7227);
## Contig21996 11364 11365 0.666667 Contig21996 maker gene 40640 47940 . - . ID=OLUR_00000754;Name=OLUR_00000754;Alias=maker-Contig21996-snap-gene-0.4;Note=Similar to rost: Protein rolling stone (Drosophila melanogaster OX%3D7227);
## Contig22322 7442 7443 0.75 . . . -1 -1 . . . .
## Contig22356 75467 75468 0.666667 Contig22356 maker gene 57021 75003 . - . ID=OLUR_00000315;Name=OLUR_00000315;Alias=maker-Contig22356-snap-gene-0.12;Note=Similar to Pgghg: Protein-glucosylgalactosylhydroxylysine glucosidase (Mus musculus OX%3D10090);Dbxref=Gene3D:G3DSA:1.50.10.10,InterPro:IPR005195,InterPro:IPR008928,InterPro:IPR012341,Pfam:PF03632,Pfam:PF10222,SUPERFAMILY:SSF48208;Ontology_term=GO:0003824,GO:0005975;
## Contig22918 4368 4369 0.692308 . . . -1 -1 . . . .
## Contig22978 6936 6937 1 . . . -1 -1 . . . .
## Contig23388 24267 24268 1 Contig23388 maker gene 11365 42804 . - . ID=OLUR_00001683;Name=OLUR_00001683;Alias=maker-Contig23388-snap-gene-0.7;Note=Similar to Decr1: 2%2C4-dienoyl-CoA reductase%2C mitochondrial (Mus musculus OX%3D10090);Dbxref=Gene3D:G3DSA:3.40.50.720,InterPro:IPR002347,InterPro:IPR036291,PRINTS:PR00081,Pfam:PF00106,Pfam:PF13561,SUPERFAMILY:SSF51735;
## Contig23388 24267 24268 1 Contig23388 maker gene 19795 29066 . + . ID=OLUR_00001684;Name=OLUR_00001684;Alias=maker-Contig23388-snap-gene-0.5;Note=Protein of unknown function;
## Contig23868 3342 3343 0.692308 . . . -1 -1 . . . .
## Contig24582 2214 2215 0.75 Contig24582 maker gene 13971 22296 . - . ID=OLUR_00008184;Name=OLUR_00008184;Alias=maker-Contig24582-snap-gene-0.2;Note=Protein of unknown function;Dbxref=InterPro:IPR008906,Pfam:PF05699;Ontology_term=GO:0046983;
## Contig24582 2255 2256 0.666667 Contig24582 maker gene 13971 22296 . - . ID=OLUR_00008184;Name=OLUR_00008184;Alias=maker-Contig24582-snap-gene-0.2;Note=Protein of unknown function;Dbxref=InterPro:IPR008906,Pfam:PF05699;Ontology_term=GO:0046983;
## Contig24822 1350 1351 0.666667 Contig24822 maker gene 258 701 . - . ID=OLUR_00019678;Name=OLUR_00019678;Alias=maker-Contig24822-snap-gene-0.7;Note=Protein of unknown function;Dbxref=Coils:Coil,MobiDBLite:mobidb-lite;
## Contig25698 6055 6056 1 Contig25698 maker gene 16 15782 . + . ID=OLUR_00014543;Name=OLUR_00014543;Alias=snap_masked-Contig25698-processed-gene-0.0;Note=Similar to SULT3A1: Amine sulfotransferase (Oryctolagus cuniculus OX%3D9986);Dbxref=Gene3D:G3DSA:3.40.50.300,InterPro:IPR000863,InterPro:IPR027417,Pfam:PF00685,SUPERFAMILY:SSF52540;Ontology_term=GO:0008146;
## Contig26090 19101 19102 1 . . . -1 -1 . . . .
## Contig26654 201 202 0.704188 Contig26654 maker gene 3314 16745 . - . ID=OLUR_00007570;Name=OLUR_00007570;Alias=maker-Contig26654-snap-gene-0.1;Note=Similar to slc39a14: Zinc transporter ZIP14 (Xenopus tropicalis OX%3D8364);Dbxref=InterPro:IPR003689,Pfam:PF02535;Ontology_term=GO:0016020,GO:0030001,GO:0046873,GO:0055085;
## Contig26892 65 66 0.666667 . . . -1 -1 . . . .
## Contig27333 907 908 0.666667 Contig27333 maker gene 34923 45546 . + . ID=OLUR_00002326;Name=OLUR_00002326;Alias=maker-Contig27333-snap-gene-0.6;Note=Similar to Nkd1: Protein naked cuticle homolog 1 (Mus musculus OX%3D10090);Dbxref=Gene3D:G3DSA:1.10.238.10,InterPro:IPR002048,InterPro:IPR011992,MobiDBLite:mobidb-lite,ProSiteProfiles:PS50222,SUPERFAMILY:SSF47473;Ontology_term=GO:0005509;
## Contig27692 10267 10268 0.666667 Contig27692 maker gene 28576 30386 . - . ID=OLUR_00004663;Name=OLUR_00004663;Alias=maker-Contig27692-snap-gene-0.5;Note=Protein of unknown function;Dbxref=Coils:Coil;
## Contig28539 17475 17476 0.882353 . . . -1 -1 . . . .
## Contig28713 4401 4402 1 . . . -1 -1 . . . .
## Contig28874 3698 3699 1 Contig28874 maker gene 702 16955 . - . ID=OLUR_00001858;Name=OLUR_00001858;Alias=maker-Contig28874-snap-gene-0.3;Note=Protein of unknown function;Dbxref=Coils:Coil,Gene3D:G3DSA:3.40.50.300,InterPro:IPR009003,InterPro:IPR027417,SUPERFAMILY:SSF50494,SUPERFAMILY:SSF52540;
## Contig29480 4376 4377 0.666667 Contig29480 maker gene 828 13486 . - . ID=OLUR_00013125;Name=OLUR_00013125;Alias=maker-Contig29480-snap-gene-0.5;Note=Protein of unknown function;Dbxref=CDD:cd00112,Gene3D:G3DSA:2.60.120.290,Gene3D:G3DSA:4.10.400.10,InterPro:IPR000859,InterPro:IPR002172,InterPro:IPR023415,InterPro:IPR035914,InterPro:IPR036055,MobiDBLite:mobidb-lite,Pfam:PF00057,ProSitePatterns:PS01209,ProSiteProfiles:PS01180,ProSiteProfiles:PS50068,SMART:SM00192,SUPERFAMILY:SSF49854,SUPERFAMILY:SSF57424;Ontology_term=GO:0005515;
## Contig29480 4406 4407 0.666667 Contig29480 maker gene 828 13486 . - . ID=OLUR_00013125;Name=OLUR_00013125;Alias=maker-Contig29480-snap-gene-0.5;Note=Protein of unknown function;Dbxref=CDD:cd00112,Gene3D:G3DSA:2.60.120.290,Gene3D:G3DSA:4.10.400.10,InterPro:IPR000859,InterPro:IPR002172,InterPro:IPR023415,InterPro:IPR035914,InterPro:IPR036055,MobiDBLite:mobidb-lite,Pfam:PF00057,ProSitePatterns:PS01209,ProSiteProfiles:PS01180,ProSiteProfiles:PS50068,SMART:SM00192,SUPERFAMILY:SSF49854,SUPERFAMILY:SSF57424;Ontology_term=GO:0005515;
## Contig304 15305 15306 1 Contig304 maker gene 725 18190 . + . ID=OLUR_00002143;Name=OLUR_00002143;Alias=maker-Contig304-snap-gene-0.4;Note=Similar to Hexb: Beta-hexosaminidase subunit beta (Rattus norvegicus OX%3D10116);Dbxref=Gene3D:G3DSA:3.20.20.80,Gene3D:G3DSA:3.30.379.10,InterPro:IPR015883,InterPro:IPR017853,InterPro:IPR025705,InterPro:IPR029018,PIRSF:PIRSF001093,PRINTS:PR00738,Pfam:PF00728,SUPERFAMILY:SSF51445,SUPERFAMILY:SSF55545;Ontology_term=GO:0004553,GO:0004563,GO:0005975;
## Contig30602 27065 27066 1 Contig30602 maker gene 298 11916 . + . ID=OLUR_00007140;Name=OLUR_00007140;Alias=maker-Contig30602-snap-gene-0.6;Note=Similar to MPPED1: Metallophosphoesterase domain-containing protein 1 (Homo sapiens OX%3D9606);Dbxref=CDD:cd07379,Gene3D:G3DSA:3.60.21.10,InterPro:IPR004843,InterPro:IPR029052,Pfam:PF00149,SUPERFAMILY:SSF56300;Ontology_term=GO:0016787;
## Contig31143 4174 4175 0.714286 . . . -1 -1 . . . .
## Contig3129 11945 11946 0.666667 Contig3129 maker gene 2925 11607 . + . ID=OLUR_00011085;Name=OLUR_00011085;Alias=maker-Contig3129-snap-gene-0.3;Note=Protein of unknown function;Dbxref=Gene3D:G3DSA:1.10.238.10,InterPro:IPR011992,SUPERFAMILY:SSF47473;
## Contig31293 6151 6152 0.666667 Contig31293 maker gene 328 25666 . - . ID=OLUR_00006187;Name=OLUR_00006187;Alias=maker-Contig31293-snap-gene-0.2;Note=Similar to Cask: Peripheral plasma membrane protein CASK (Mus musculus OX%3D10090);Dbxref=CDD:cd00992,Gene3D:G3DSA:1.10.287.650,Gene3D:G3DSA:2.30.30.40,InterPro:IPR036028,InterPro:IPR036034,MobiDBLite:mobidb-lite,SUPERFAMILY:SSF50044,SUPERFAMILY:SSF50156;Ontology_term=GO:0005515;
## Contig318 36726 36727 0.666667 Contig318 maker gene 23781 37296 . - . ID=OLUR_00003526;Name=OLUR_00003526;Alias=maker-Contig318-snap-gene-0.5;Note=Similar to PNKP: Bifunctional polynucleotide phosphatase/kinase (Homo sapiens OX%3D9606);Dbxref=CDD:cd00060,Gene3D:G3DSA:2.60.200.20,Gene3D:G3DSA:3.40.50.1000,InterPro:IPR000253,InterPro:IPR006549,InterPro:IPR008984,InterPro:IPR013954,InterPro:IPR023214,InterPro:IPR036412,MobiDBLite:mobidb-lite,Pfam:PF08645,SUPERFAMILY:SSF49879,SUPERFAMILY:SSF56784,TIGRFAM:TIGR01662;Ontology_term=GO:0005515;
## Contig32856 531 532 0.714286 . . . -1 -1 . . . .
## Contig32988 87 88 1 . . . -1 -1 . . . .
## Contig33032 9531 9532 0.666667 . . . -1 -1 . . . .
## Contig33550 2589 2590 0.666667 Contig33550 maker gene 2242 8418 . + . ID=OLUR_00011261;Name=OLUR_00011261;Alias=snap_masked-Contig33550-processed-gene-0.0;Note=Protein of unknown function;
## Contig34150 5173 5174 1 Contig34150 maker gene 2857 4985 . - . ID=OLUR_00027570;Name=OLUR_00027570;Alias=maker-Contig34150-snap-gene-0.1;Note=Similar to Helz2: Helicase with zinc finger domain 2 (Mus musculus OX%3D10090);Dbxref=Gene3D:G3DSA:3.40.50.300,InterPro:IPR027417,Pfam:PF13087,SUPERFAMILY:SSF52540;
## Contig35054 6216 6217 0.666667 . . . -1 -1 . . . .
## Contig35054 6311 6312 0.666667 . . . -1 -1 . . . .
## Contig35054 6326 6327 0.666667 . . . -1 -1 . . . .
## Contig35923 1800 1801 0.714286 Contig35923 maker gene 842 2655 . + . ID=OLUR_00015888;Name=OLUR_00015888;Alias=maker-Contig35923-snap-gene-0.3;Note=Similar to Zdhhc22: Palmitoyltransferase ZDHHC22 (Rattus norvegicus OX%3D10116);Dbxref=InterPro:IPR001594,Pfam:PF01529,ProSiteProfiles:PS50216;
## Contig36110 12157 12158 0.666667 Contig36110 maker gene 86 11835 . + . ID=OLUR_00013834;Name=OLUR_00013834;Alias=snap_masked-Contig36110-processed-gene-0.0;Note=Similar to AATF: Protein AATF (Gallus gallus OX%3D9031);Dbxref=Coils:Coil,InterPro:IPR012617,InterPro:IPR025160,MobiDBLite:mobidb-lite,Pfam:PF08164,Pfam:PF13339;Ontology_term=GO:0005634;
## Contig36147 5397 5398 0.666667 . . . -1 -1 . . . .
## Contig36147 5399 5400 0.666667 . . . -1 -1 . . . .
## Contig36147 5694 5695 0.666667 . . . -1 -1 . . . .
## Contig36147 5769 5770 0.666667 . . . -1 -1 . . . .
## Contig37611 1343 1344 0.692308 . . . -1 -1 . . . .
## Contig37849 128 129 1 Contig37849 maker gene 382 12169 . - . ID=OLUR_00012535;Name=OLUR_00012535;Alias=maker-Contig37849-snap-gene-0.3;Note=Similar to CRIP1: Cysteine-rich protein 1 (Bos taurus OX%3D9913);Dbxref=CDD:cd09401,Gene3D:G3DSA:2.10.110.10,InterPro:IPR001781,MobiDBLite:mobidb-lite,Pfam:PF00412,ProSiteProfiles:PS50023,SMART:SM00132,SUPERFAMILY:SSF57716;
## Contig39097 4194 4195 0.72028 Contig39097 maker gene 845 4039 . - . ID=OLUR_00028187;Name=OLUR_00028187;Alias=maker-Contig39097-snap-gene-0.1;Note=Protein of unknown function;
## Contig391 37575 37576 0.666667 Contig391 maker gene 10709 28646 . - . ID=OLUR_00002891;Name=OLUR_00002891;Alias=maker-Contig391-snap-gene-0.3;Note=Similar to ZMIZ1: Zinc finger MIZ domain-containing protein 1 (Homo sapiens OX%3D9606);Dbxref=CDD:cd16791,Gene3D:G3DSA:3.30.40.10,InterPro:IPR004181,InterPro:IPR013083,MobiDBLite:mobidb-lite,Pfam:PF02891,ProSiteProfiles:PS51044;Ontology_term=GO:0008270;
## Contig39314 6846 6847 1 . . . -1 -1 . . . .
## Contig41772 9196 9197 1 . . . -1 -1 . . . .
## Contig42560 6180 6181 0.666667 Contig42560 maker gene 33 7302 . + . ID=OLUR_00022801;Name=OLUR_00022801;Alias=maker-Contig42560-snap-gene-0.2;Note=Protein of unknown function;Dbxref=InterPro:IPR036859,MobiDBLite:mobidb-lite,SUPERFAMILY:SSF74924;
## Contig432 6613 6614 0.666667 Contig432 maker gene 961 3817 . - . ID=OLUR_00003935;Name=OLUR_00003935;Alias=maker-Contig432-snap-gene-0.6;Note=Similar to pgap2: Post-GPI attachment to proteins factor 2 (Xenopus tropicalis OX%3D8364);Dbxref=InterPro:IPR019402,Pfam:PF10277;
## Contig43516 15396 15397 1 Contig43516 maker gene 9261 57829 . - . ID=OLUR_00000508;Name=OLUR_00000508;Alias=snap_masked-Contig43516-processed-gene-0.3;Note=Protein of unknown function;Dbxref=CDD:cd00063,Coils:Coil,InterPro:IPR003961,InterPro:IPR036116,SUPERFAMILY:SSF49265;Ontology_term=GO:0005515;
## Contig43953 5268 5269 1 Contig43953 maker gene 5874 10056 . + . ID=OLUR_00020866;Name=OLUR_00020866;Alias=maker-Contig43953-snap-gene-0.2;Note=Similar to MTHFS: 5-formyltetrahydrofolate cyclo-ligase (Homo sapiens OX%3D9606);Dbxref=Gene3D:G3DSA:3.40.50.10420,InterPro:IPR002698,InterPro:IPR024185,InterPro:IPR037171,PIRSF:PIRSF006806,Pfam:PF01812,SUPERFAMILY:SSF100950,TIGRFAM:TIGR02727;
## Contig44003 16800 16801 0.818182 Contig44003 maker gene 2779 13457 . + . ID=OLUR_00012393;Name=OLUR_00012393;Alias=maker-Contig44003-snap-gene-0.2;Note=Similar to ZNF91: Zinc finger protein 91 (Homo sapiens OX%3D9606);Dbxref=Coils:Coil,Gene3D:G3DSA:3.30.160.60,InterPro:IPR013087,InterPro:IPR036236,MobiDBLite:mobidb-lite,Pfam:PF00096,ProSitePatterns:PS00028,ProSiteProfiles:PS50157,SMART:SM00355,SUPERFAMILY:SSF57667;Ontology_term=GO:0003676;
## Contig44322 23253 23254 1 Contig44322 maker gene 13879 23228 . + . ID=OLUR_00009320;Name=OLUR_00009320;Alias=snap_masked-Contig44322-processed-gene-0.0;Note=Protein of unknown function;Dbxref=Gene3D:G3DSA:2.170.300.10,MobiDBLite:mobidb-lite;
## Contig44322 23255 23256 1 Contig44322 maker gene 13879 23228 . + . ID=OLUR_00009320;Name=OLUR_00009320;Alias=snap_masked-Contig44322-processed-gene-0.0;Note=Protein of unknown function;Dbxref=Gene3D:G3DSA:2.170.300.10,MobiDBLite:mobidb-lite;
## Contig44384 9178 9179 1 Contig44384 maker gene 6407 16623 . - . ID=OLUR_00009552;Name=OLUR_00009552;Alias=maker-Contig44384-snap-gene-0.3;Note=Similar to Grcc10: Protein C10 (Mus musculus OX%3D10090);Dbxref=InterPro:IPR026317,Pfam:PF14974;
## Contig46385 18311 18312 1 Contig46385 maker gene 1528 11048 . - . ID=OLUR_00008699;Name=OLUR_00008699;Alias=maker-Contig46385-snap-gene-0.2;Note=Similar to G-protein coupled receptor GRL101 (Lymnaea stagnalis OX%3D6523);Dbxref=Gene3D:G3DSA:3.80.10.10,InterPro:IPR001611,InterPro:IPR003591,InterPro:IPR032675,Pfam:PF13855,SMART:SM00369,SUPERFAMILY:SSF52058;Ontology_term=GO:0005515;
## Contig46507 4415 4416 0.666667 Contig46507 maker gene 133 6160 . + . ID=OLUR_00023641;Name=OLUR_00023641;Alias=maker-Contig46507-snap-gene-0.1;Note=Similar to T25G3.4: Probable glycerol-3-phosphate dehydrogenase%2C mitochondrial (Caenorhabditis elegans OX%3D6239);Dbxref=CDD:cd00051,Gene3D:G3DSA:1.10.238.10,Gene3D:G3DSA:1.10.8.870,Gene3D:G3DSA:3.50.50.60,InterPro:IPR000447,InterPro:IPR002048,InterPro:IPR011992,InterPro:IPR031656,InterPro:IPR036188,InterPro:IPR038299,Pfam:PF13499,Pfam:PF16901,ProSitePatterns:PS00978,ProSiteProfiles:PS50222,SUPERFAMILY:SSF47473;Ontology_term=GO:0004368,GO:0005509,GO:0006072,GO:0009331,GO:0055114;
## Contig46507 4519 4520 0.666667 Contig46507 maker gene 133 6160 . + . ID=OLUR_00023641;Name=OLUR_00023641;Alias=maker-Contig46507-snap-gene-0.1;Note=Similar to T25G3.4: Probable glycerol-3-phosphate dehydrogenase%2C mitochondrial (Caenorhabditis elegans OX%3D6239);Dbxref=CDD:cd00051,Gene3D:G3DSA:1.10.238.10,Gene3D:G3DSA:1.10.8.870,Gene3D:G3DSA:3.50.50.60,InterPro:IPR000447,InterPro:IPR002048,InterPro:IPR011992,InterPro:IPR031656,InterPro:IPR036188,InterPro:IPR038299,Pfam:PF13499,Pfam:PF16901,ProSitePatterns:PS00978,ProSiteProfiles:PS50222,SUPERFAMILY:SSF47473;Ontology_term=GO:0004368,GO:0005509,GO:0006072,GO:0009331,GO:0055114;
## Contig46507 4583 4584 0.666667 Contig46507 maker gene 133 6160 . + . ID=OLUR_00023641;Name=OLUR_00023641;Alias=maker-Contig46507-snap-gene-0.1;Note=Similar to T25G3.4: Probable glycerol-3-phosphate dehydrogenase%2C mitochondrial (Caenorhabditis elegans OX%3D6239);Dbxref=CDD:cd00051,Gene3D:G3DSA:1.10.238.10,Gene3D:G3DSA:1.10.8.870,Gene3D:G3DSA:3.50.50.60,InterPro:IPR000447,InterPro:IPR002048,InterPro:IPR011992,InterPro:IPR031656,InterPro:IPR036188,InterPro:IPR038299,Pfam:PF13499,Pfam:PF16901,ProSitePatterns:PS00978,ProSiteProfiles:PS50222,SUPERFAMILY:SSF47473;Ontology_term=GO:0004368,GO:0005509,GO:0006072,GO:0009331,GO:0055114;
## Contig46507 4592 4593 0.666667 Contig46507 maker gene 133 6160 . + . ID=OLUR_00023641;Name=OLUR_00023641;Alias=maker-Contig46507-snap-gene-0.1;Note=Similar to T25G3.4: Probable glycerol-3-phosphate dehydrogenase%2C mitochondrial (Caenorhabditis elegans OX%3D6239);Dbxref=CDD:cd00051,Gene3D:G3DSA:1.10.238.10,Gene3D:G3DSA:1.10.8.870,Gene3D:G3DSA:3.50.50.60,InterPro:IPR000447,InterPro:IPR002048,InterPro:IPR011992,InterPro:IPR031656,InterPro:IPR036188,InterPro:IPR038299,Pfam:PF13499,Pfam:PF16901,ProSitePatterns:PS00978,ProSiteProfiles:PS50222,SUPERFAMILY:SSF47473;Ontology_term=GO:0004368,GO:0005509,GO:0006072,GO:0009331,GO:0055114;
## Contig47095 172 173 1 . . . -1 -1 . . . .
## Contig47348 4826 4827 0.666667 Contig47348 maker gene 3040 7119 . - . ID=OLUR_00021713;Name=OLUR_00021713;Alias=snap_masked-Contig47348-processed-gene-0.2;Note=Protein of unknown function;Dbxref=MobiDBLite:mobidb-lite;
## Contig47661 16814 16815 0.75 Contig47661 maker gene 305 11690 . + . ID=OLUR_00010754;Name=OLUR_00010754;Alias=snap_masked-Contig47661-processed-gene-0.0;Note=Similar to Slc16a14: Monocarboxylate transporter 14 (Mus musculus OX%3D10090);Dbxref=CDD:cd06174,Gene3D:G3DSA:1.20.1250.20,InterPro:IPR011701,InterPro:IPR020846,InterPro:IPR036259,MobiDBLite:mobidb-lite,Pfam:PF07690,SUPERFAMILY:SSF103473;Ontology_term=GO:0016021,GO:0055085;
## Contig480 24883 24884 0.714286 Contig480 maker gene 789 28994 . + . ID=OLUR_00002279;Name=OLUR_00002279;Alias=maker-Contig480-snap-gene-0.7;Note=Similar to DDX19A: ATP-dependent RNA helicase DDX19A (Bos taurus OX%3D9913);Dbxref=CDD:cd00079,Gene3D:G3DSA:3.40.50.300,InterPro:IPR001650,InterPro:IPR011545,InterPro:IPR014001,InterPro:IPR027417,MobiDBLite:mobidb-lite,Pfam:PF00270,Pfam:PF00271,ProSiteProfiles:PS51192,ProSiteProfiles:PS51194,SMART:SM00487,SMART:SM00490,SUPERFAMILY:SSF52540;Ontology_term=GO:0003676,GO:0005524;
## Contig48372 241 242 0.666667 Contig48372 maker gene 1837 5846 . + . ID=OLUR_00020932;Name=OLUR_00020932;Alias=maker-Contig48372-snap-gene-0.1;Note=Similar to mth2: G-protein coupled receptor Mth2 (Drosophila yakuba OX%3D7245);Dbxref=CDD:cd15039,Gene3D:G3DSA:1.20.1070.10,InterPro:IPR000832,InterPro:IPR017981,Pfam:PF00002,ProSiteProfiles:PS50261;Ontology_term=GO:0004888,GO:0004930,GO:0007166,GO:0007186,GO:0016021;
## Contig49154 2089 2090 0.714286 . . . -1 -1 . . . .
## Contig4937 1948 1949 1 Contig4937 maker gene 476 2727 . + . ID=OLUR_00016163;Name=OLUR_00016163;Alias=maker-Contig4937-snap-gene-0.1;Note=Protein of unknown function;
## Contig5093 2469 2470 1 Contig5093 maker gene 3369 3968 . + . ID=OLUR_00025752;Name=OLUR_00025752;Alias=snap_masked-Contig5093-processed-gene-0.0;Note=Similar to Liver carboxylesterase 4 (Rattus norvegicus OX%3D10116);Dbxref=Gene3D:G3DSA:3.40.50.1820,InterPro:IPR002018,InterPro:IPR029058,Pfam:PF00135,SUPERFAMILY:SSF53474;
## Contig5093 2489 2490 0.692308 Contig5093 maker gene 3369 3968 . + . ID=OLUR_00025752;Name=OLUR_00025752;Alias=snap_masked-Contig5093-processed-gene-0.0;Note=Similar to Liver carboxylesterase 4 (Rattus norvegicus OX%3D10116);Dbxref=Gene3D:G3DSA:3.40.50.1820,InterPro:IPR002018,InterPro:IPR029058,Pfam:PF00135,SUPERFAMILY:SSF53474;
## Contig511 19789 19790 1 Contig511 maker gene 7963 8495 . - . ID=OLUR_00006306;Name=OLUR_00006306;Alias=maker-Contig511-snap-gene-0.2;Note=Protein of unknown function;
## Contig51106 3045 3046 0.666667 . . . -1 -1 . . . .
## Contig51106 3051 3052 0.666667 . . . -1 -1 . . . .
## Contig51132 20121 20122 0.666667 Contig51132 maker gene 4087 12195 . - . ID=OLUR_00010594;Name=OLUR_00010594;Alias=snap_masked-Contig51132-processed-gene-0.0;Note=Protein of unknown function;
## Contig51516 568 569 0.666667 Contig51516 maker gene 1657 7648 . + . ID=OLUR_00012624;Name=OLUR_00012624;Alias=maker-Contig51516-snap-gene-0.1;Note=Protein of unknown function;Dbxref=MobiDBLite:mobidb-lite;
## Contig52305 6069 6070 1 . . . -1 -1 . . . .
## Contig52453 9292 9293 0.666667 . . . -1 -1 . . . .
## Contig5323 2566 2567 0.666667 . . . -1 -1 . . . .
## Contig53490 0 1 0.666667 . . . -1 -1 . . . .
## Contig53554 2587 2588 1 . . . -1 -1 . . . .
## Contig53554 2721 2722 1 . . . -1 -1 . . . .
## Contig54121 12063 12064 0.666667 . . . -1 -1 . . . .
## Contig55264 3969 3970 1 Contig55264 maker gene 2482 4249 . - . ID=OLUR_00028246;Name=OLUR_00028246;Alias=maker-Contig55264-snap-gene-0.1;Note=Similar to LIPG: Endothelial lipase (Homo sapiens OX%3D9606);Dbxref=Gene3D:G3DSA:2.60.60.20,Gene3D:G3DSA:3.40.50.1820,InterPro:IPR000734,InterPro:IPR001024,InterPro:IPR013818,InterPro:IPR029058,InterPro:IPR036392,PRINTS:PR00821,Pfam:PF00151,ProSiteProfiles:PS50095,SUPERFAMILY:SSF49723,SUPERFAMILY:SSF53474;Ontology_term=GO:0005515,GO:0052689;
## Contig55874 2111 2112 0.742188 . . . -1 -1 . . . .
## Contig56012 8382 8383 1 Contig56012 maker gene 7002 26653 . + . ID=OLUR_00005481;Name=OLUR_00005481;Alias=maker-Contig56012-snap-gene-0.5;Note=Similar to UBR4: E3 ubiquitin-protein ligase UBR4 (Homo sapiens OX%3D9606);Dbxref=Coils:Coil,InterPro:IPR016024,InterPro:IPR025704,Pfam:PF13764,SUPERFAMILY:SSF48371;
## Contig56827 8472 8473 1 Contig56827 maker gene 8440 24419 . - . ID=OLUR_00006427;Name=OLUR_00006427;Alias=maker-Contig56827-snap-gene-0.2;Note=Similar to Fbn2: Fibrillin-2 (Mus musculus OX%3D10090);Dbxref=CDD:cd00054,Gene3D:G3DSA:2.10.25.10,InterPro:IPR000152,InterPro:IPR000742,InterPro:IPR001881,InterPro:IPR009030,InterPro:IPR013032,InterPro:IPR018097,Pfam:PF07645,ProSitePatterns:PS00010,ProSitePatterns:PS01186,ProSitePatterns:PS01187,ProSiteProfiles:PS50026,SMART:SM00179,SMART:SM00181,SUPERFAMILY:SSF57184;Ontology_term=GO:0005509;
## Contig56856 8859 8860 1 Contig56856 maker gene 3168 20548 . - . ID=OLUR_00011021;Name=OLUR_00011021;Alias=maker-Contig56856-snap-gene-0.2;Note=Similar to NLGN4Y: Neuroligin-4%2C Y-linked (Homo sapiens OX%3D9606);Dbxref=Coils:Coil,Gene3D:G3DSA:3.40.50.1820,InterPro:IPR002018,InterPro:IPR029058,Pfam:PF00135,SUPERFAMILY:SSF53474;
## Contig57203 7247 7248 1 . . . -1 -1 . . . .
## Contig57463 3836 3837 1 Contig57463 maker gene 4529 15007 . + . ID=OLUR_00014642;Name=OLUR_00014642;Alias=maker-Contig57463-snap-gene-0.2;Note=Similar to Rmnd5a: E3 ubiquitin-protein ligase RMND5A (Mus musculus OX%3D10090);Dbxref=CDD:cd16652,InterPro:IPR006594,InterPro:IPR013144,InterPro:IPR024964,InterPro:IPR027370,InterPro:IPR037683,Pfam:PF10607,Pfam:PF13445,ProSiteProfiles:PS50896,SMART:SM00757,SUPERFAMILY:SSF57850;Ontology_term=GO:0005515;
## Contig57864 6940 6941 0.666667 . . . -1 -1 . . . .
## Contig57864 6953 6954 0.666667 . . . -1 -1 . . . .
## Contig57864 7025 7026 0.818182 . . . -1 -1 . . . .
## Contig57991 4001 4002 1 Contig57991 maker gene 130 2331 . + . ID=OLUR_00028721;Name=OLUR_00028721;Alias=maker-Contig57991-snap-gene-0.2;Note=Protein of unknown function;Dbxref=Gene3D:G3DSA:1.20.1270.280,InterPro:IPR004273,Pfam:PF03028;Ontology_term=GO:0003777,GO:0007018,GO:0030286;
## Contig58366 390 391 1 . . . -1 -1 . . . .
## Contig61792 2821 2822 0.818182 . . . -1 -1 . . . .
## Contig62058 6907 6908 0.714286 . . . -1 -1 . . . .
## Contig6250 3912 3913 0.666667 . . . -1 -1 . . . .
## Contig627815 67 68 1 . . . -1 -1 . . . .
## Contig63441 3879 3880 0.666667 . . . -1 -1 . . . .
## Contig64 42055 42056 0.692308 Contig64 maker gene 24516 49627 . - . ID=OLUR_00001856;Name=OLUR_00001856;Alias=maker-Contig64-snap-gene-0.14;Note=Similar to RIC1: RAB6A-GEF complex partner protein 1 (Homo sapiens OX%3D9606);Dbxref=Gene3D:G3DSA:2.130.10.10,InterPro:IPR009771,InterPro:IPR015943,InterPro:IPR036322,MobiDBLite:mobidb-lite,Pfam:PF07064,SUPERFAMILY:SSF50978;Ontology_term=GO:0005515;
## Contig65281 1849 1850 0.714286 . . . -1 -1 . . . .
## Contig66485 760 761 0.666667 . . . -1 -1 . . . .
## Contig6668 3298 3299 1 . . . -1 -1 . . . .
## Contig67089 4659 4660 0.714286 . . . -1 -1 . . . .
## Contig67464 11938 11939 0.666667 . . . -1 -1 . . . .
## Contig67464 12046 12047 0.666667 . . . -1 -1 . . . .
## Contig67464 12088 12089 0.666667 . . . -1 -1 . . . .
## Contig67464 12175 12176 0.666667 . . . -1 -1 . . . .
## Contig67464 12183 12184 0.666667 . . . -1 -1 . . . .
## Contig695 3336 3337 0.818182 Contig695 maker gene 32 19666 . + . ID=OLUR_00011341;Name=OLUR_00011341;Alias=maker-Contig695-snap-gene-0.3;Note=Similar to ttn-1: Titin homolog (Caenorhabditis elegans OX%3D6239);Dbxref=CDD:cd00096,Coils:Coil,Gene3D:G3DSA:2.60.40.10,InterPro:IPR003598,InterPro:IPR003599,InterPro:IPR007110,InterPro:IPR013098,InterPro:IPR013783,InterPro:IPR022189,InterPro:IPR036179,MobiDBLite:mobidb-lite,Pfam:PF07679,Pfam:PF12510,ProSiteProfiles:PS50835,SMART:SM00408,SMART:SM00409,SUPERFAMILY:SSF48726;
## Contig699 18759 18760 0.666667 Contig699 maker gene 232 23732 . - . ID=OLUR_00006741;Name=OLUR_00006741;Alias=maker-Contig699-snap-gene-0.3;Note=Similar to acj6: Inhibitory POU protein (Drosophila melanogaster OX%3D7227);Dbxref=CDD:cd00086,CDD:cd00093,Coils:Coil,Gene3D:G3DSA:1.10.10.60,Gene3D:G3DSA:1.10.260.40,InterPro:IPR000327,InterPro:IPR001356,InterPro:IPR001387,InterPro:IPR009057,InterPro:IPR010982,InterPro:IPR013847,InterPro:IPR017970,PRINTS:PR00028,Pfam:PF00046,Pfam:PF00157,ProSitePatterns:PS00027,ProSitePatterns:PS00035,ProSitePatterns:PS00465,ProSiteProfiles:PS50071,ProSiteProfiles:PS51179,SMART:SM00352,SMART:SM00389,SUPERFAMILY:SSF46689,SUPERFAMILY:SSF47413;Ontology_term=GO:0003677,GO:0003700,GO:0006355,GO:0043565;
## Contig70917 3271 3272 1 . . . -1 -1 . . . .
## Contig70917 3272 3273 1 . . . -1 -1 . . . .
## Contig7140 779 780 0.714286 . . . -1 -1 . . . .
## Contig71660 4145 4146 0.666667 . . . -1 -1 . . . .
## Contig7407 572 573 0.753425 . . . -1 -1 . . . .
## Contig76889 1491 1492 1 . . . -1 -1 . . . .
## Contig78568 677 678 0.818182 . . . -1 -1 . . . .
## Contig80273 871 872 0.666667 . . . -1 -1 . . . .
## Contig82737 793 794 1 Contig82737 maker gene 124 12486 . - . ID=OLUR_00016587;Name=OLUR_00016587;Alias=maker-Contig82737-snap-gene-0.2;Note=Protein of unknown function;Dbxref=Gene3D:G3DSA:1.25.10.10,InterPro:IPR011989,InterPro:IPR016024,MobiDBLite:mobidb-lite,SUPERFAMILY:SSF48371;
## Contig82923 7507 7508 0.714286 . . . -1 -1 . . . .
## Contig84075 4315 4316 0.666667 Contig84075 maker gene 4304 5784 . + . ID=OLUR_00019752;Name=OLUR_00019752;Alias=snap_masked-Contig84075-processed-gene-0.0;Note=Similar to drpr: Protein draper (Drosophila melanogaster OX%3D7227);Dbxref=Gene3D:G3DSA:2.170.300.10,InterPro:IPR000742,ProSiteProfiles:PS50026;
## Contig84075 4422 4423 0.666667 Contig84075 maker gene 4304 5784 . + . ID=OLUR_00019752;Name=OLUR_00019752;Alias=snap_masked-Contig84075-processed-gene-0.0;Note=Similar to drpr: Protein draper (Drosophila melanogaster OX%3D7227);Dbxref=Gene3D:G3DSA:2.170.300.10,InterPro:IPR000742,ProSiteProfiles:PS50026;
## Contig84075 4427 4428 0.666667 Contig84075 maker gene 4304 5784 . + . ID=OLUR_00019752;Name=OLUR_00019752;Alias=snap_masked-Contig84075-processed-gene-0.0;Note=Similar to drpr: Protein draper (Drosophila melanogaster OX%3D7227);Dbxref=Gene3D:G3DSA:2.170.300.10,InterPro:IPR000742,ProSiteProfiles:PS50026;
## Contig84075 4510 4511 0.666667 Contig84075 maker gene 4304 5784 . + . ID=OLUR_00019752;Name=OLUR_00019752;Alias=snap_masked-Contig84075-processed-gene-0.0;Note=Similar to drpr: Protein draper (Drosophila melanogaster OX%3D7227);Dbxref=Gene3D:G3DSA:2.170.300.10,InterPro:IPR000742,ProSiteProfiles:PS50026;
## Contig84217 344 345 1 . . . -1 -1 . . . .
## Contig85743 5392 5393 1 . . . -1 -1 . . . .
## Contig87773 2443 2444 1 . . . -1 -1 . . . .
## Contig89811 3749 3750 0.666667 . . . -1 -1 . . . .
## Contig89811 3754 3755 0.666667 . . . -1 -1 . . . .
## Contig9 94520 94521 1 Contig9 maker gene 68213 103748 . - . ID=OLUR_00000054;Name=OLUR_00000054;Alias=maker-Contig9-snap-gene-0.16;Note=Similar to bli-3: Dual oxidase 1 (Caenorhabditis elegans OX%3D6239);Dbxref=Gene3D:G3DSA:1.10.640.10,InterPro:IPR010255,InterPro:IPR013130,InterPro:IPR019791,InterPro:IPR037120,MobiDBLite:mobidb-lite,Pfam:PF01794,Pfam:PF03098,ProSiteProfiles:PS50292,SUPERFAMILY:SSF48113;Ontology_term=GO:0004601,GO:0006979,GO:0020037,GO:0055114;
## Contig90533 1382 1383 0.666667 . . . -1 -1 . . . .
## Contig91113 3659 3660 0.714286 . . . -1 -1 . . . .
## Contig91113 3665 3666 0.714286 . . . -1 -1 . . . .
## Contig91113 3666 3667 0.714286 . . . -1 -1 . . . .
## Contig91150 520 521 0.666667 . . . -1 -1 . . . .
## Contig97712 15219 15220 0.666667 . . . -1 -1 . . . .
## Contig97712 15247 15248 0.666667 . . . -1 -1 . . . .