Objective: This analysis investigates washed A. niger spore germination dynamics that were pre-treated with glucose across various durations (2h–12h) + cysteine or its analogue, like N-acetyl cysteine or S-methyl cysteine.

📥 Load and Filter Data

🔬 Germination State Classification

📊 Summarize Germination & Swelling

A control experiment was conducted where A. niger spores were pre-treated with glucose (instead of proline) for 2–12 hours, followed by exposure to cysteine or its analogues. While Proline pre-treatment combined with cysteine strongly inhibits germination, glucose pre-treatment, in contrast, does not fully block germination, even in the presence of cysteine. This suggests that cysteine’s inhibitory effect is potentiated by prior activation with proline, and may be linked to metabolic state or readiness to grow. Met Cys and Acetyl Cys again show minimal to no inhibition, regardless of pre-treatment type.

📈 Swelling % Over Time

📈 Germination % Over Time