MT_Clase 5
Regina Hernández
2025-02-26
Instalar y correr la paquetería
install.packages("pacman")## Installing package into '/cloud/lib/x86_64-pc-linux-gnu-library/4.4'
## (as 'lib' is unspecified)library("pacman")
p_load("readr", "dplyr", "ggplot2","ggrepel", "matrixTests")Cargar la base de datos
PCR <- read_csv(file = "https://raw.githubusercontent.com/ManuelLaraMVZ/Transcript-mica/main/datos_miRNAs.csv")## Rows: 363 Columns: 8
## ── Column specification ────────────────────────────────────────────────────────
## Delimiter: ","
## chr (2): Gen, Condicion
## dbl (6): Cx1, Cx2, Cx3, T1, T2, T3
##
## ℹ Use `spec()` to retrieve the full column specification for this data.
## ℹ Specify the column types or set `show_col_types = FALSE` to quiet this message.head(PCR)## # A tibble: 6 × 8
## Gen Condicion Cx1 Cx2 Cx3 T1 T2 T3
## <chr> <chr> <dbl> <dbl> <dbl> <dbl> <dbl> <dbl>
## 1 U6 snRNA-001973 Control 13.8 11.7 11.9 13.2 13.0 12.6
## 2 ath-miR159a-000338 Target 35 35 35 35 35 35
## 3 hsa-let-7a-000377 Target 20.5 21.0 21.0 20.4 19.6 20.9
## 4 hsa-let-7b-002619 Target 18.4 19.0 19.1 18.3 17.4 19.0
## 5 hsa-let-7c-000379 Target 22.2 23.7 23.8 22.9 22.0 24.0
## 6 hsa-let-7d-002283 Target 22.2 22.7 21.9 22.0 21.2 21.9Genref <- PCR %>%
filter(Condicion == "Control") %>%
select(-2)
Genref## # A tibble: 3 × 7
## Gen Cx1 Cx2 Cx3 T1 T2 T3
## <chr> <dbl> <dbl> <dbl> <dbl> <dbl> <dbl>
## 1 U6 snRNA-001973 13.8 11.7 11.9 13.2 13.0 12.6
## 2 RNU44-001094 17.3 16.9 16.8 17.7 17.3 16.8
## 3 RNU48-001006 15.1 16.4 17.0 16.0 15.1 16.4Selección de datos
Genint <- PCR %>%
filter(Condicion == "Target") %>%
select(-2)
Genint## # A tibble: 360 × 7
## Gen Cx1 Cx2 Cx3 T1 T2 T3
## <chr> <dbl> <dbl> <dbl> <dbl> <dbl> <dbl>
## 1 ath-miR159a-000338 35 35 35 35 35 35
## 2 hsa-let-7a-000377 20.5 21.0 21.0 20.4 19.6 20.9
## 3 hsa-let-7b-002619 18.4 19.0 19.1 18.3 17.4 19.0
## 4 hsa-let-7c-000379 22.2 23.7 23.8 22.9 22.0 24.0
## 5 hsa-let-7d-002283 22.2 22.7 21.9 22.0 21.2 21.9
## 6 hsa-let-7e-002406 18.0 18.4 18.5 18.0 17.3 18.6
## 7 hsa-let-7f-000382 24.4 25.2 24.7 23.8 23.0 24.9
## 8 hsa-let-7g-002282 22.1 22.1 22.5 22.3 22.0 22.8
## 9 hsa-miR-1-002222 31.4 32.9 32.5 33.0 31.8 33.6
## 10 hsa-miR-100-000437 17.2 16.4 16.3 16.9 17.0 16.0
## # ℹ 350 more rowsCorrer los promedios de cada columna de los genes control
meanCx1 <- mean(Genref$Cx1)
meanCx2 <- mean(Genref$Cx2)
meanCx3 <- mean(Genref$Cx3)
meanT1 <- mean(Genref$T1)
meanT2 <- mean(Genref$T2)
meanT3 <- mean(Genref$T3)Sacar el Delta Ct de cada columna de los genes de interés
DCt <- Genint %>%
mutate(DCT_CX1 = Cx1-meanCx1,
DCT_CX2 = Cx2-meanCx2,
DCT_CX3 = Cx3-meanCx3,
DCT_T1 = T1-meanT1,
DCT_T2 = T2-meanT2,
DCT_T3 = T3-meanT3) %>%
select (-2,-3,-4,-5,-6,-7)DosDeltaCT
DosDCt <- DCt %>%
mutate(A1 = 2^-(DCT_CX1),
A2 = 2^-(DCT_CX2),
A3 = 2^-(DCT_CX3),
B1 = 2^-(DCT_T1),
B2 = 2^-(DCT_T2),
B3 = 2^-(DCT_T3)) %>%
select (-2:-7)
DosDCt## # A tibble: 360 × 7
## Gen A1 A2 A3 B1 B2 B3
## <chr> <dbl> <dbl> <dbl> <dbl> <dbl> <dbl>
## 1 ath-miR159a-000338 0.00000124 0.000000960 0.00000114 1.47e-6 1.03e-6 1.16e-6
## 2 hsa-let-7a-000377 0.0289 0.0157 0.0185 3.70e-2 4.49e-2 1.98e-2
## 3 hsa-let-7b-002619 0.127 0.0615 0.0677 1.62e-1 1.98e-1 7.75e-2
## 4 hsa-let-7c-000379 0.00900 0.00243 0.00272 6.40e-3 8.67e-3 2.39e-3
## 5 hsa-let-7d-002283 0.00893 0.00492 0.0102 1.23e-2 1.46e-2 9.87e-3
## 6 hsa-let-7e-002406 0.167 0.0960 0.108 1.91e-1 2.21e-1 1.03e-1
## 7 hsa-let-7f-000382 0.00190 0.000882 0.00146 3.58e-3 4.25e-3 1.24e-3
## 8 hsa-let-7g-002282 0.00963 0.00720 0.00659 9.86e-3 8.59e-3 5.50e-3
## 9 hsa-miR-1-002222 0.0000150 0.00000404 0.00000661 5.99e-6 9.45e-6 3.04e-6
## 10 hsa-miR-100-000437 0.287 0.390 0.498 4.10e-1 2.69e-1 6.25e-1
## # ℹ 350 more rowsEstadística para obtener los valores de p, hacemos t de Student
Tstudent <-row_t_welch(DosDCt[,c("A1","A2","A3")],
DosDCt[,c("B1","B2","B3")])
Tstudent## obs.x obs.y obs.tot mean.x mean.y mean.diff var.x
## 1 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 2 3 3 6 2.104945e-02 3.390214e-02 -1.285269e-02 4.856207e-05
## 3 3 3 6 8.558097e-02 1.457383e-01 -6.015735e-02 1.327202e-03
## 4 3 3 6 4.716674e-03 5.820710e-03 -1.104036e-03 1.377166e-05
## 5 3 3 6 8.019463e-03 1.226943e-02 -4.249971e-03 7.633725e-06
## 6 3 3 6 1.234439e-01 1.716779e-01 -4.823397e-02 1.436199e-03
## 7 3 3 6 1.415957e-03 3.020536e-03 -1.604579e-03 2.628669e-07
## 8 3 3 6 7.807440e-03 7.982499e-03 -1.750587e-04 2.597538e-06
## 9 3 3 6 8.564286e-06 6.161569e-06 2.402717e-06 3.308511e-11
## 10 3 3 6 3.915742e-01 4.344875e-01 -4.291324e-02 1.114555e-02
## 11 3 3 6 4.660299e-05 6.171893e-05 -1.511595e-05 1.656519e-09
## 12 3 3 6 2.105688e-03 3.364205e-03 -1.258517e-03 5.126217e-06
## 13 3 3 6 6.064444e-05 2.889903e-05 3.174541e-05 2.659358e-09
## 14 3 3 6 5.827561e-02 3.869783e-02 1.957778e-02 1.317081e-03
## 15 3 3 6 2.651212e-03 2.320379e-03 3.308328e-04 1.369858e-08
## 16 3 3 6 9.009825e-05 1.455156e-04 -5.541731e-05 7.126923e-09
## 17 3 3 6 3.021619e-03 4.583025e-03 -1.561406e-03 1.266358e-06
## 18 3 3 6 5.039671e-03 2.110901e-03 2.928770e-03 1.914134e-05
## 19 3 3 6 7.054721e-05 1.431738e-05 5.622983e-05 3.899013e-09
## 20 3 3 6 1.474855e-04 1.063027e-04 4.118283e-05 2.027013e-09
## 21 3 3 6 1.490942e-02 1.255904e-02 2.350377e-03 6.097556e-05
## 22 3 3 6 2.468989e-01 2.171538e-01 2.974509e-02 1.226340e-03
## 23 3 3 6 2.229028e-03 4.281092e-03 -2.052064e-03 1.428867e-05
## 24 3 3 6 1.467177e-02 2.884515e-02 -1.417338e-02 5.404866e-04
## 25 3 3 6 2.001403e-06 4.145193e-06 -2.143790e-06 2.733803e-12
## 26 3 3 6 2.328888e-04 2.764702e-04 -4.358139e-05 2.743895e-08
## 27 3 3 6 8.713056e-05 1.220085e-06 8.591047e-05 5.539929e-09
## 28 3 3 6 1.020972e-03 2.156915e-03 -1.135943e-03 7.603694e-07
## 29 3 3 6 1.183985e-03 1.132401e-03 5.158369e-05 1.909076e-09
## 30 3 3 6 3.158725e-03 6.170534e-03 -3.011809e-03 8.179060e-07
## 31 3 3 6 9.172217e-05 1.121953e-04 -2.047318e-05 4.972909e-10
## 32 3 3 6 2.280642e-06 6.119194e-06 -3.838552e-06 4.564845e-12
## 33 3 3 6 1.767978e-06 5.094298e-06 -3.326320e-06 6.051348e-13
## 34 3 3 6 1.200044e-04 6.128161e-05 5.872276e-05 3.709139e-09
## 35 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 36 3 3 6 1.952591e-02 1.451481e-02 5.011100e-03 5.082350e-05
## 37 3 3 6 4.079707e-04 1.220085e-06 4.067506e-04 2.076098e-07
## 38 3 3 6 9.116368e-04 6.812789e-04 2.303579e-04 1.879137e-07
## 39 3 3 6 4.693988e-04 4.679298e-04 1.468984e-06 2.091530e-07
## 40 3 3 6 3.621335e-06 2.705340e-06 9.159951e-07 1.193731e-11
## 41 3 3 6 7.156152e-06 7.274462e-06 -1.183098e-07 1.952018e-11
## 42 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 43 3 3 6 8.299544e-03 1.452195e-02 -6.222408e-03 2.007974e-04
## 44 3 3 6 1.129087e-01 2.378820e-01 -1.249733e-01 3.771082e-02
## 45 3 3 6 2.187186e-02 2.836711e-02 -6.495258e-03 9.904670e-04
## 46 3 3 6 2.631937e-03 2.205920e-03 4.260172e-04 1.367392e-07
## 47 3 3 6 1.085420e-05 3.865732e-06 6.988467e-06 2.718606e-10
## 48 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 49 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 50 3 3 6 3.509529e-06 1.937164e-05 -1.586211e-05 1.155347e-11
## 51 3 3 6 7.078916e-04 1.009646e-03 -3.017549e-04 5.668111e-07
## 52 3 3 6 4.480454e-03 5.963192e-03 -1.482738e-03 2.597399e-05
## 53 3 3 6 3.214382e-05 2.619738e-05 5.946445e-06 2.273221e-10
## 54 3 3 6 5.046997e-03 8.963832e-03 -3.916835e-03 2.378752e-05
## 55 3 3 6 1.262725e-05 4.270356e-05 -3.007631e-05 4.022349e-10
## 56 3 3 6 8.818195e-03 3.696279e-03 5.121916e-03 4.426351e-05
## 57 3 3 6 2.117778e-04 2.353264e-04 -2.354857e-05 1.332210e-07
## 58 3 3 6 1.593581e-02 1.070549e-02 5.230318e-03 5.135405e-05
## 59 3 3 6 3.981672e-02 2.921243e-02 1.060430e-02 2.162328e-04
## 60 3 3 6 6.369867e-02 4.012625e-02 2.357241e-02 1.671772e-03
## 61 3 3 6 6.118135e-03 9.510845e-03 -3.392711e-03 8.565969e-06
## 62 3 3 6 1.566179e-04 4.585830e-04 -3.019651e-04 2.756121e-08
## 63 3 3 6 1.957846e-05 1.292733e-05 6.651126e-06 2.039694e-10
## 64 3 3 6 1.292494e-05 9.320515e-06 3.604424e-06 1.318781e-10
## 65 3 3 6 1.108877e-04 4.080095e-04 -2.971217e-04 6.018821e-10
## 66 3 3 6 2.977965e-04 3.457686e-04 -4.797212e-05 1.760280e-08
## 67 3 3 6 9.673608e-04 2.637622e-03 -1.670261e-03 4.214000e-07
## 68 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 69 3 3 6 4.066661e-04 2.554589e-04 1.512072e-04 5.693991e-10
## 70 3 3 6 5.716871e-04 3.570702e-04 2.146169e-04 9.170895e-08
## 71 3 3 6 3.491119e-06 1.607591e-05 -1.258480e-05 1.110713e-11
## 72 3 3 6 1.312038e-01 9.761514e-02 3.358862e-02 5.864687e-03
## 73 3 3 6 2.564648e-04 2.981544e-04 -4.168969e-05 1.336172e-08
## 74 3 3 6 4.012472e-06 2.770622e-06 1.241850e-06 1.250912e-11
## 75 3 3 6 3.495032e-03 4.875330e-03 -1.380298e-03 8.492783e-06
## 76 3 3 6 3.843566e-02 4.824988e-02 -9.814220e-03 8.090405e-04
## 77 3 3 6 2.493132e-04 2.268784e-04 2.243484e-05 6.097772e-09
## 78 3 3 6 2.366282e-03 2.562475e-03 -1.961933e-04 2.339040e-06
## 79 3 3 6 8.767159e-06 1.829742e-05 -9.530260e-06 2.060034e-11
## 80 3 3 6 9.553235e-03 1.094639e-02 -1.393157e-03 2.704496e-06
## 81 3 3 6 4.506514e-06 1.171377e-05 -7.207254e-06 3.588867e-11
## 82 3 3 6 5.662058e-05 1.020761e-04 -4.545547e-05 9.264895e-09
## 83 3 3 6 1.762669e-02 2.782106e-02 -1.019437e-02 9.306778e-04
## 84 3 3 6 8.146476e-06 2.983074e-05 -2.168427e-05 9.178744e-11
## 85 3 3 6 2.830998e-03 2.196840e-03 6.341583e-04 2.360447e-07
## 86 3 3 6 1.526783e-01 9.159685e-02 6.108143e-02 4.064813e-03
## 87 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 88 3 3 6 1.645045e-04 1.341367e-04 3.036781e-05 7.249879e-09
## 89 3 3 6 1.858238e-03 1.102432e-03 7.558058e-04 1.323531e-06
## 90 3 3 6 1.113319e-06 3.396051e-06 -2.282732e-06 2.043512e-14
## 91 3 3 6 9.046523e-06 2.027808e-05 -1.123156e-05 4.703234e-11
## 92 3 3 6 1.572139e-03 1.068165e-03 5.039740e-04 1.401195e-06
## 93 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 94 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 95 3 3 6 1.113319e-06 4.910555e-06 -3.797237e-06 2.043512e-14
## 96 3 3 6 3.421626e-02 2.019890e-02 1.401736e-02 4.772999e-04
## 97 3 3 6 2.935019e-03 1.909496e-03 1.025522e-03 3.473426e-06
## 98 3 3 6 1.254300e-01 1.223965e-01 3.033553e-03 8.364281e-04
## 99 3 3 6 1.959684e-02 1.636564e-02 3.231195e-03 2.118334e-04
## 100 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 101 3 3 6 2.988557e-04 4.673837e-04 -1.685280e-04 9.469914e-09
## 102 3 3 6 8.665431e-03 1.395343e-02 -5.288003e-03 2.252146e-04
## 103 3 3 6 3.479147e-04 9.283720e-05 2.550775e-04 1.205426e-07
## 104 3 3 6 1.113319e-06 1.328614e-06 -2.152948e-07 2.043512e-14
## 105 3 3 6 2.038940e-06 1.220085e-06 8.188548e-07 2.653318e-12
## 106 3 3 6 1.492118e-06 6.320263e-06 -4.828144e-06 5.990311e-13
## 107 3 3 6 2.883704e-03 2.766802e-03 1.169013e-04 2.503693e-07
## 108 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 109 3 3 6 6.222207e-06 2.346467e-06 3.875741e-06 2.016879e-11
## 110 3 3 6 6.026598e-02 5.405958e-02 6.206393e-03 1.089558e-02
## 111 3 3 6 8.611206e-03 9.573077e-03 -9.618700e-04 3.195577e-05
## 112 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 113 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 114 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 115 3 3 6 4.693119e-02 4.582263e-02 1.108560e-03 1.465090e-04
## 116 3 3 6 4.424726e-01 4.600090e-01 -1.753644e-02 4.219504e-02
## 117 3 3 6 9.727350e-05 1.022794e-04 -5.005929e-06 3.537674e-10
## 118 3 3 6 7.099274e-04 8.785103e-04 -1.685830e-04 7.690968e-07
## 119 3 3 6 9.088273e-03 1.576501e-02 -6.676742e-03 2.846418e-05
## 120 3 3 6 1.113319e-06 2.012492e-03 -2.011379e-03 2.043512e-14
## 121 3 3 6 5.378424e-01 1.056140e+00 -5.182977e-01 4.586881e-01
## 122 3 3 6 2.163373e-03 1.698624e-03 4.647493e-04 1.872906e-06
## 123 3 3 6 2.022700e-02 2.450926e-02 -4.282259e-03 1.776354e-05
## 124 3 3 6 1.070440e-03 3.747011e-03 -2.676571e-03 4.983670e-07
## 125 3 3 6 1.101605e-02 1.450768e-02 -3.491637e-03 1.266018e-04
## 126 3 3 6 2.872583e-03 4.513280e-03 -1.640698e-03 8.961287e-06
## 127 3 3 6 1.835742e-03 2.168553e-03 -3.328118e-04 4.847296e-07
## 128 3 3 6 3.864376e-03 3.880236e-03 -1.585999e-05 1.211406e-06
## 129 3 3 6 4.463163e-03 3.370490e-03 1.092673e-03 1.264586e-05
## 130 3 3 6 2.510926e-05 1.153820e-05 1.357106e-05 2.057936e-10
## 131 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 132 3 3 6 1.113319e-06 1.508766e-06 -3.954476e-07 2.043512e-14
## 133 3 3 6 6.145714e-05 1.096505e-04 -4.819333e-05 1.037518e-08
## 134 3 3 6 5.497109e-01 3.207144e-01 2.289966e-01 9.826625e-02
## 135 3 3 6 2.168998e-04 2.171407e-04 -2.408342e-07 1.953673e-09
## 136 3 3 6 1.802701e-03 2.579534e-03 -7.768333e-04 6.399369e-06
## 137 3 3 6 2.495361e-04 3.021789e-04 -5.264274e-05 1.400174e-07
## 138 3 3 6 6.585870e-06 1.001658e-05 -3.430712e-06 6.229050e-11
## 139 3 3 6 1.462546e-04 2.264062e-04 -8.015159e-05 4.974473e-08
## 140 3 3 6 1.579179e-04 1.144045e-03 -9.861267e-04 7.127099e-08
## 141 3 3 6 1.104892e-01 1.339909e-01 -2.350170e-02 8.044955e-03
## 142 3 3 6 3.897033e-02 4.675922e-02 -7.788885e-03 1.145312e-05
## 143 3 3 6 5.251704e-02 5.884372e-02 -6.326682e-03 1.418191e-04
## 144 3 3 6 5.581470e-02 9.051045e-02 -3.469575e-02 9.345491e-03
## 145 3 3 6 6.442323e-06 2.936527e-06 3.505797e-06 3.348862e-12
## 146 3 3 6 2.006619e-02 2.059337e-02 -5.271786e-04 2.746923e-08
## 147 3 3 6 6.047101e-04 7.832379e-04 -1.785279e-04 9.850914e-07
## 148 3 3 6 1.265270e-03 1.518319e-03 -2.530491e-04 2.922660e-07
## 149 3 3 6 3.416374e-04 4.375052e-04 -9.586777e-05 1.043964e-07
## 150 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 151 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 152 3 3 6 1.656729e-03 3.032267e-03 -1.375538e-03 2.089774e-06
## 153 3 3 6 2.477035e-05 5.973684e-05 -3.496649e-05 8.432102e-10
## 154 3 3 6 1.673251e-04 3.429880e-04 -1.756630e-04 4.107824e-08
## 155 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 156 3 3 6 1.378009e-02 2.129449e-02 -7.514400e-03 3.375875e-04
## 157 3 3 6 1.008717e-05 9.400241e-06 6.869283e-07 2.827106e-11
## 158 3 3 6 1.723003e-03 1.921316e-03 -1.983128e-04 1.385409e-07
## 159 3 3 6 1.778412e-04 2.025436e-04 -2.470234e-05 9.018652e-08
## 160 3 3 6 2.377099e-06 1.220085e-06 1.157014e-06 4.232245e-12
## 161 3 3 6 1.088510e-03 1.043931e-03 4.457883e-05 8.432850e-09
## 162 3 3 6 3.112940e-03 3.873780e-03 -7.608398e-04 9.705059e-07
## 163 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 164 3 3 6 3.140216e-05 4.961281e-05 -1.821065e-05 2.746781e-09
## 165 3 3 6 3.192331e-03 4.064337e-03 -8.720063e-04 7.621320e-06
## 166 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 167 3 3 6 2.521315e-03 2.047291e-03 4.740239e-04 3.040298e-07
## 168 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 169 3 3 6 1.066543e-02 7.603593e-03 3.061837e-03 3.124576e-06
## 170 3 3 6 9.566917e-05 9.463770e-05 1.031471e-06 4.907280e-09
## 171 3 3 6 3.287745e-04 5.303568e-04 -2.015823e-04 2.382690e-09
## 172 3 3 6 3.592237e-04 2.947947e-04 6.442904e-05 1.742033e-09
## 173 3 3 6 3.264266e-05 2.687865e-05 5.764013e-06 1.822061e-10
## 174 3 3 6 1.246140e-05 2.207287e-05 -9.611474e-06 3.907948e-10
## 175 3 3 6 7.609206e-03 9.077444e-03 -1.468238e-03 7.840560e-05
## 176 3 3 6 1.123093e-02 2.239284e-02 -1.116190e-02 2.368991e-05
## 177 3 3 6 2.819020e-06 1.998213e-05 -1.716311e-05 9.415698e-12
## 178 3 3 6 1.691764e-05 1.054283e-05 6.374812e-06 3.228539e-10
## 179 3 3 6 3.850705e-03 5.475806e-03 -1.625101e-03 4.145038e-05
## 180 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 181 3 3 6 1.856060e-05 1.083237e-05 7.728223e-06 2.393982e-12
## 182 3 3 6 1.697266e-06 4.257064e-06 -2.559798e-06 1.271771e-12
## 183 3 3 6 4.276599e-03 4.738730e-03 -4.621314e-04 8.589792e-06
## 184 3 3 6 1.906605e-05 2.482551e-05 -5.759465e-06 3.092866e-10
## 185 3 3 6 4.924531e-03 9.289115e-03 -4.364585e-03 5.235829e-05
## 186 3 3 6 2.563099e-02 1.377008e-02 1.186091e-02 5.328969e-05
## 187 3 3 6 9.443363e-03 1.681934e-02 -7.375982e-03 2.024626e-04
## 188 3 3 6 2.326727e-05 1.745335e-05 5.813925e-06 1.481077e-09
## 189 3 3 6 2.682884e-05 4.662936e-05 -1.980052e-05 1.993940e-09
## 190 3 3 6 6.959233e-06 2.182582e-05 -1.486659e-05 8.089942e-11
## 191 3 3 6 6.616579e-03 1.174727e-02 -5.130689e-03 1.069374e-04
## 192 3 3 6 1.272272e-05 1.802695e-05 -5.304239e-06 4.088946e-10
## 193 3 3 6 1.232945e-05 1.220085e-06 1.110937e-05 3.722812e-10
## 194 3 3 6 1.036533e-04 1.142616e-04 -1.060831e-05 2.347744e-08
## 195 3 3 6 2.104035e-04 5.446268e-04 -3.342233e-04 1.203774e-07
## 196 3 3 6 3.277631e-03 5.838822e-03 -2.561191e-03 2.547590e-05
## 197 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 198 3 3 6 7.558659e-05 6.534867e-05 1.023792e-05 2.729662e-10
## 199 3 3 6 1.851999e-04 1.739710e-04 1.122887e-05 4.027727e-10
## 200 3 3 6 2.991780e-05 2.576480e-05 4.153004e-06 1.401043e-10
## 201 3 3 6 1.987509e-03 1.347572e-03 6.399367e-04 1.132586e-06
## 202 3 3 6 2.356642e-06 7.277357e-06 -4.920714e-06 5.144197e-12
## 203 3 3 6 1.470146e-02 8.658886e-03 6.042571e-03 6.271578e-05
## 204 3 3 6 2.986909e-04 3.811315e-04 -8.244057e-05 9.664869e-08
## 205 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 206 3 3 6 7.853671e-05 2.569319e-05 5.284351e-05 4.757415e-09
## 207 3 3 6 1.359544e-04 5.995955e-05 7.599487e-05 1.340849e-08
## 208 3 3 6 2.746382e-05 2.396792e-05 3.495892e-06 8.153107e-11
## 209 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 210 3 3 6 1.833489e-04 1.220085e-06 1.821289e-04 9.970076e-08
## 211 3 3 6 2.021992e-04 3.851986e-04 -1.829994e-04 1.213853e-07
## 212 3 3 6 1.994559e-06 4.615059e-06 -2.620499e-06 2.694798e-12
## 213 3 3 6 1.736076e-03 2.370238e-03 -6.341611e-04 3.079926e-07
## 214 3 3 6 4.620865e-04 4.705514e-04 -8.464879e-06 5.469734e-08
## 215 3 3 6 1.650690e-03 1.693147e-03 -4.245633e-05 1.578092e-07
## 216 3 3 6 1.302962e-05 3.209576e-05 -1.906614e-05 2.892493e-11
## 217 3 3 6 2.506614e-02 2.041189e-02 4.654255e-03 6.174418e-06
## 218 3 3 6 5.927666e-04 7.934285e-04 -2.006619e-04 8.878205e-07
## 219 3 3 6 4.402049e-04 1.080345e-03 -6.401400e-04 5.785759e-07
## 220 3 3 6 4.463964e-03 1.097548e-02 -6.511518e-03 4.885986e-05
## 221 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 222 3 3 6 9.699816e-05 1.601238e-04 -6.312564e-05 2.341797e-08
## 223 3 3 6 9.187918e-04 1.581845e-03 -6.630537e-04 2.253253e-06
## 224 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 225 3 3 6 6.500821e-05 9.863529e-05 -3.362708e-05 5.667242e-11
## 226 3 3 6 5.149176e-06 3.697278e-05 -3.182360e-05 5.046309e-11
## 227 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 228 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 229 3 3 6 2.849564e-04 4.790057e-04 -1.940493e-04 2.294908e-07
## 230 3 3 6 2.439836e-03 3.675543e-03 -1.235707e-03 1.378051e-05
## 231 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 232 3 3 6 1.557839e-04 1.460205e-04 9.763440e-06 7.760480e-11
## 233 3 3 6 5.923033e-04 4.116824e-04 1.806209e-04 8.407177e-08
## 234 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 235 3 3 6 2.346162e-05 5.573403e-05 -3.227240e-05 1.805844e-10
## 236 3 3 6 8.392283e-05 5.318567e-05 3.073715e-05 1.006784e-10
## 237 3 3 6 3.655390e-05 3.720113e-05 -6.472363e-07 1.408835e-11
## 238 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 239 3 3 6 1.290358e-04 2.300930e-04 -1.010573e-04 1.555332e-08
## 240 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 241 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 242 3 3 6 1.804753e-06 3.321077e-06 -1.516323e-06 1.725066e-12
## 243 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 244 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 245 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 246 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 247 3 3 6 3.625215e-04 1.046245e-03 -6.837238e-04 3.919890e-07
## 248 3 3 6 1.855712e-04 1.061946e-04 7.937655e-05 2.549928e-08
## 249 3 3 6 1.124104e-06 1.220085e-06 -9.598165e-08 1.583773e-14
## 250 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 251 3 3 6 1.250987e-03 1.252552e-03 -1.564260e-06 9.587017e-08
## 252 3 3 6 1.113319e-06 1.300331e-06 -1.870124e-07 2.043512e-14
## 253 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 254 3 3 6 5.890077e-06 4.943810e-06 9.462667e-07 2.897755e-11
## 255 3 3 6 9.841591e-05 5.349501e-05 4.492090e-05 7.130928e-09
## 256 3 3 6 1.113319e-06 7.211851e-06 -6.098532e-06 2.043512e-14
## 257 3 3 6 8.563837e-05 7.988558e-05 5.752796e-06 4.900977e-09
## 258 3 3 6 9.986171e-06 6.784016e-06 3.202155e-06 6.914627e-11
## 259 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 260 3 3 6 4.927528e-05 1.908788e-04 -1.416035e-04 1.901351e-09
## 261 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 262 3 3 6 4.172429e-06 7.355474e-06 -3.183045e-06 2.669189e-11
## 263 3 3 6 6.548868e-02 2.609142e-02 3.939726e-02 4.084306e-03
## 264 3 3 6 1.911001e-05 8.590351e-06 1.051966e-05 2.562929e-10
## 265 3 3 6 2.050200e-05 1.769280e-05 2.809198e-06 3.438976e-10
## 266 3 3 6 5.365736e-05 3.315702e-05 2.050033e-05 1.878864e-09
## 267 3 3 6 2.492327e-06 1.220085e-06 1.272242e-06 2.445870e-12
## 268 3 3 6 3.084810e-05 2.299162e-05 7.856479e-06 6.584266e-10
## 269 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 270 3 3 6 4.300339e-06 1.220085e-06 3.080253e-06 2.903005e-11
## 271 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 272 3 3 6 3.546635e-06 7.346583e-06 -3.799948e-06 4.352846e-12
## 273 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 274 3 3 6 1.113319e-06 9.163317e-05 -9.051985e-05 2.043512e-14
## 275 3 3 6 1.113319e-06 3.759437e-06 -2.646118e-06 2.043512e-14
## 276 3 3 6 5.006405e-05 1.490186e-05 3.516219e-05 2.162951e-09
## 277 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 278 3 3 6 1.298722e-05 1.436338e-05 -1.376155e-06 1.064344e-10
## 279 3 3 6 6.492716e-04 1.646419e-03 -9.971478e-04 4.993665e-07
## 280 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 281 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 282 3 3 6 5.665072e-06 1.963353e-06 3.701718e-06 2.440035e-11
## 283 3 3 6 6.590259e-06 2.244252e-06 4.346007e-06 9.038121e-11
## 284 3 3 6 2.791691e-03 2.684388e-03 1.073030e-04 1.687529e-07
## 285 3 3 6 3.751986e-04 4.827417e-04 -1.075431e-04 6.465611e-09
## 286 3 3 6 2.469100e-03 5.793979e-03 -3.324879e-03 1.587005e-05
## 287 3 3 6 1.716957e-05 3.553418e-05 -1.836461e-05 7.797089e-10
## 288 3 3 6 5.618388e-06 7.738291e-06 -2.119902e-06 9.507823e-12
## 289 3 3 6 3.455165e-06 3.326331e-06 1.288344e-07 4.736645e-12
## 290 3 3 6 1.558006e-06 1.232863e-06 3.251438e-07 7.875711e-13
## 291 3 3 6 1.452242e-06 1.946790e-06 -4.945481e-07 4.975769e-13
## 292 3 3 6 2.622192e-06 1.220085e-06 1.402106e-06 5.504112e-12
## 293 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 294 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 295 3 3 6 1.415553e-05 1.038295e-05 3.772583e-06 1.356392e-10
## 296 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 297 3 3 6 3.872955e-06 1.832163e-06 2.040792e-06 1.354430e-12
## 298 3 3 6 2.764661e-05 1.940046e-06 2.570657e-05 1.326934e-09
## 299 3 3 6 1.082231e-05 2.953399e-06 7.868915e-06 1.665316e-11
## 300 3 3 6 1.522184e-05 1.752448e-05 -2.302637e-06 2.128049e-10
## 301 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 302 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 303 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 304 3 3 6 3.324252e-06 1.220085e-06 2.104167e-06 3.252226e-12
## 305 3 3 6 1.986129e-02 4.589151e-02 -2.603022e-02 6.772929e-04
## 306 3 3 6 5.995882e-05 4.810164e-05 1.185719e-05 1.775995e-10
## 307 3 3 6 1.860189e-05 1.322000e-05 5.381884e-06 9.244093e-10
## 308 3 3 6 7.477902e-05 6.970456e-05 5.074460e-06 4.690743e-11
## 309 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 310 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 311 3 3 6 2.330712e-05 2.814967e-05 -4.842547e-06 1.193832e-10
## 312 3 3 6 1.442121e-03 1.303298e-03 1.388230e-04 1.332392e-06
## 313 3 3 6 2.521571e-05 2.836104e-05 -3.145331e-06 3.077235e-11
## 314 3 3 6 3.736097e-04 6.914940e-04 -3.178843e-04 1.672668e-07
## 315 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 316 3 3 6 5.353315e-05 4.127526e-05 1.225790e-05 3.792352e-10
## 317 3 3 6 1.279049e-04 1.030913e-04 2.481354e-05 3.399194e-09
## 318 3 3 6 1.494592e-05 1.220085e-06 1.372583e-05 2.727171e-10
## 319 3 3 6 1.582679e-04 5.899024e-05 9.927768e-05 1.859104e-08
## 320 3 3 6 2.124926e-06 3.990878e-06 -1.865952e-06 1.212043e-13
## 321 3 3 6 1.447372e-04 4.505505e-04 -3.058133e-04 1.553770e-08
## 322 3 3 6 2.762487e-05 2.144790e-05 6.176974e-06 2.212071e-10
## 323 3 3 6 7.471521e-05 5.420566e-05 2.050955e-05 6.758087e-10
## 324 3 3 6 9.102159e-05 9.760878e-05 -6.587191e-06 2.997761e-10
## 325 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 326 3 3 6 4.925494e-04 3.373312e-04 1.552182e-04 2.298972e-08
## 327 3 3 6 1.113319e-06 1.508831e-06 -3.955127e-07 2.043512e-14
## 328 3 3 6 4.103673e-04 1.073716e-03 -6.633488e-04 3.815234e-07
## 329 3 3 6 3.405333e-05 1.168125e-04 -8.275918e-05 2.158474e-09
## 330 3 3 6 4.932086e-04 1.000630e-03 -5.074209e-04 5.338944e-07
## 331 3 3 6 1.234216e-03 1.409233e-03 -1.750171e-04 7.177501e-07
## 332 3 3 6 1.024943e-03 7.364802e-04 2.884623e-04 3.319524e-07
## 333 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 334 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 335 3 3 6 1.207291e-03 1.183563e-03 2.372771e-05 5.710057e-07
## 336 3 3 6 1.620595e-03 3.246413e-03 -1.625817e-03 3.157383e-06
## 337 3 3 6 1.716533e-04 2.940137e-04 -1.223605e-04 5.039681e-08
## 338 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 339 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 340 3 3 6 1.113319e-06 1.832955e-06 -7.196360e-07 2.043512e-14
## 341 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 342 3 3 6 3.292857e-06 1.220085e-06 2.072771e-06 3.901551e-12
## 343 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 344 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 345 3 3 6 1.997082e-05 1.791560e-05 2.055215e-06 9.326529e-11
## 346 3 3 6 2.153793e-03 1.722886e-03 4.309063e-04 4.007983e-07
## 347 3 3 6 2.971253e-04 3.157829e-04 -1.865763e-05 3.731366e-09
## 348 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 349 3 3 6 1.172847e-01 1.239761e-01 -6.691428e-03 5.292181e-04
## 350 3 3 6 2.657208e-04 4.461779e-04 -1.804571e-04 1.402138e-07
## 351 3 3 6 1.650194e-03 1.163444e-03 4.867503e-04 1.721168e-06
## 352 3 3 6 1.024474e-05 3.972844e-06 6.271899e-06 6.355345e-11
## 353 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 354 3 3 6 1.113319e-06 1.220085e-06 -1.067666e-07 2.043512e-14
## 355 3 3 6 2.653678e-02 1.300463e-02 1.353215e-02 5.362932e-04
## 356 3 3 6 1.798639e-02 7.939075e-03 1.004732e-02 1.655929e-04
## 357 3 3 6 4.816179e-06 3.004938e-05 -2.523320e-05 4.260196e-11
## 358 3 3 6 1.113319e-06 1.909133e-06 -7.958140e-07 2.043512e-14
## 359 3 3 6 4.390855e-01 5.584689e-01 -1.193833e-01 3.209332e-02
## 360 3 3 6 3.717849e-02 3.232857e-02 4.849922e-03 8.641327e-06
## var.y stderr df statistic pvalue conf.low
## 1 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 2 1.636924e-04 8.411390e-03 3.090675 -1.528010243 0.22135734 -3.918263e-02
## 3 3.822375e-03 4.143097e-02 3.239447 -1.451989902 0.23595971 -1.866636e-01
## 4 1.011733e-05 2.821878e-03 3.908539 -0.391241721 0.71600730 -9.011661e-03
## 5 5.688217e-06 2.107284e-03 3.916473 -2.016800135 0.11541119 -1.015027e-02
## 6 3.763194e-03 4.163089e-02 3.332494 -1.158610292 0.32285067 -1.735489e-01
## 7 2.494494e-06 9.587077e-04 2.416886 -1.673689143 0.21424898 -5.117435e-03
## 8 5.035504e-06 1.595101e-03 3.629718 -0.109747728 0.91840507 -4.787765e-03
## 9 1.030689e-11 3.803157e-06 3.135871 0.631769222 0.57060050 -9.409293e-06
## 10 3.210614e-02 1.200718e-01 3.239245 -0.357396519 0.74282647 -4.095547e-01
## 11 9.467646e-10 2.945779e-05 3.723245 -0.513139244 0.63678810 -9.935818e-05
## 12 4.637373e-06 1.804032e-03 3.989998 -0.697613584 0.52393273 -6.272267e-03
## 13 2.293148e-09 4.063048e-05 3.978248 0.781320107 0.47849563 -8.130655e-05
## 14 1.467244e-03 3.046487e-02 3.988399 0.642634672 0.55554022 -6.510337e-02
## 15 1.457525e-06 7.002912e-04 2.037591 0.472421788 0.68240012 -2.629630e-03
## 16 7.183262e-09 6.906563e-05 3.999938 -0.802386252 0.46729416 -2.471754e-04
## 17 3.888877e-06 1.310882e-03 3.177665 -1.191110775 0.31492232 -5.604318e-03
## 18 1.335186e-05 3.291059e-03 3.876922 0.889917420 0.42528375 -6.324220e-03
## 19 3.879577e-10 3.780199e-05 2.394104 1.487483430 0.25524549 -8.327301e-05
## 20 1.470305e-09 3.414341e-05 3.901150 1.206172120 0.29576933 -5.456935e-05
## 21 6.637577e-05 6.515400e-03 3.992821 0.360741831 0.73656780 -1.575211e-02
## 22 3.488419e-03 3.964324e-02 3.251516 0.750319397 0.50366260 -9.107218e-02
## 23 1.359265e-05 3.048570e-03 3.997509 -0.673123646 0.53777908 -1.051833e-02
## 24 6.761384e-04 2.013806e-02 3.950883 -0.703810608 0.52081586 -7.036082e-02
## 25 1.090042e-11 2.131840e-06 2.943826 -1.005605435 0.38996694 -9.002085e-06
## 26 2.686085e-08 1.345360e-04 3.999547 -0.323938545 0.76221692 -4.171299e-04
## 27 5.127956e-14 4.297278e-05 2.000037 1.999183436 0.18361223 -9.898320e-05
## 28 2.772062e-06 1.085116e-03 3.020415 -1.046840059 0.37161416 -4.576100e-03
## 29 1.957123e-07 2.566589e-04 2.039014 0.200981497 0.85898269 -1.032727e-03
## 30 8.537675e-06 1.765935e-03 2.379714 -1.705503505 0.20988312 -9.558666e-03
## 31 2.256771e-09 3.029886e-05 2.840604 -0.675707916 0.55013328 -1.200321e-04
## 32 6.929344e-11 4.961797e-06 2.262369 -0.773621346 0.51180775 -2.298186e-05
## 33 3.743840e-11 3.561064e-06 2.064637 -0.934080397 0.44620938 -1.819772e-05
## 34 7.089618e-09 5.999655e-05 3.643000 0.978769057 0.38812920 -1.144940e-04
## 35 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 36 1.475585e-04 8.131872e-03 3.231610 0.616229659 0.57844918 -1.984986e-02
## 37 5.127956e-14 2.630652e-04 2.000001 1.546196908 0.26210134 -7.251270e-04
## 38 1.227732e-07 3.218110e-04 3.831565 0.715817280 0.51529870 -6.788383e-04
## 39 1.365070e-07 3.394407e-04 3.830795 0.004327660 0.99676295 -9.576157e-04
## 40 2.376691e-12 2.184338e-06 2.766026 0.419346705 0.70537759 -6.380335e-06
## 41 1.086812e-10 6.537110e-06 2.695986 -0.018098187 0.98681500 -2.231455e-05
## 42 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 43 1.725299e-04 1.115538e-02 3.977198 -0.557794572 0.60688794 -3.726485e-02
## 44 4.373954e-02 1.647729e-01 3.978205 -0.758457656 0.49062721 -5.834464e-01
## 45 5.267974e-04 2.248899e-02 3.658351 -0.288819435 0.78833598 -7.130302e-02
## 46 7.990316e-08 2.687269e-04 3.742419 1.585316779 0.19291432 -3.407836e-04
## 47 1.337587e-11 9.750836e-06 2.196330 0.716704319 0.54209097 -3.156931e-05
## 48 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 49 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 50 4.446918e-11 4.321367e-06 2.973521 -3.670623439 0.03550702 -2.968417e-05
## 51 7.573794e-07 6.643770e-04 3.918837 -0.454192248 0.67370632 -2.161526e-03
## 52 2.246277e-05 4.018157e-03 3.979090 -0.369009467 0.73091656 -1.266209e-02
## 53 5.515364e-11 9.703534e-06 2.916540 0.612812272 0.58446649 -2.544052e-05
## 54 3.217999e-05 4.319240e-03 3.912035 -0.906834180 0.41685747 -1.601607e-02
## 55 1.379788e-09 2.437227e-05 3.074742 -1.234037900 0.30311962 -1.065839e-04
## 56 2.554264e-05 4.823766e-03 3.731613 1.061808567 0.35215177 -8.659582e-03
## 57 1.645939e-07 3.150740e-04 3.956098 -0.074739828 0.94404813 -9.021761e-04
## 58 6.143017e-05 6.131455e-03 3.968326 0.853030489 0.44206934 -1.184705e-02
## 59 7.580790e-05 9.866453e-03 3.248844 1.074782972 0.35573397 -1.947748e-02
## 60 1.588013e-03 3.296354e-02 3.997361 0.715105689 0.51408809 -6.797288e-02
## 61 2.018305e-05 3.095643e-03 3.438536 -1.095963098 0.34385551 -1.257040e-02
## 62 1.172552e-07 2.197092e-04 2.890986 -1.374385133 0.26617065 -1.016333e-03
## 63 1.444379e-10 1.077663e-05 3.886530 0.617180504 0.57143307 -2.361728e-05
## 64 6.821615e-11 8.166890e-06 3.632316 0.441345924 0.68394614 -2.000504e-05
## 65 2.288723e-07 2.765708e-04 2.010519 -1.074306348 0.39459231 -1.481163e-03
## 66 2.771138e-08 1.229013e-04 3.810381 -0.390330521 0.71712413 -3.960032e-04
## 67 1.000562e-06 6.884675e-04 3.430851 -2.426056825 0.08289784 -3.713548e-03
## 68 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 69 3.457246e-08 1.082310e-04 2.065861 1.397078246 0.29353603 -3.005316e-04
## 70 1.027415e-07 2.545914e-04 3.987165 0.842985607 0.44683687 -4.931405e-04
## 71 3.938817e-10 1.161879e-05 2.112707 -1.083141681 0.38676574 -6.010572e-05
## 72 4.541494e-03 5.889590e-02 3.936356 0.570304867 0.59944365 -1.309806e-01
## 73 6.916620e-09 8.221584e-05 3.633005 -0.507076077 0.64131420 -2.793455e-04
## 74 7.350711e-12 2.572925e-06 3.747194 0.482660808 0.65616759 -6.095879e-06
## 75 4.513003e-06 2.082129e-03 3.657522 -0.662926134 0.54680623 -7.381082e-03
## 76 5.056040e-04 2.093358e-02 3.797681 -0.468826604 0.66483217 -6.917691e-02
## 77 4.372059e-09 5.907574e-05 3.894202 0.379764018 0.72391984 -1.433582e-04
## 78 1.143084e-06 1.077361e-03 3.577939 -0.182105327 0.86534339 -3.331860e-03
## 79 3.938325e-10 1.175348e-05 2.208659 -0.810845793 0.49554934 -5.578800e-05
## 80 6.813860e-07 1.062369e-03 2.947630 -1.311368892 0.28252849 -4.808326e-03
## 81 1.066964e-10 6.894082e-06 3.208698 -1.045426294 0.36809870 -2.836169e-05
## 82 1.078348e-08 8.174835e-05 3.977181 -0.556041444 0.60797841 -2.729398e-04
## 83 6.325512e-04 2.282710e-02 3.859621 -0.446590607 0.67907768 -7.449208e-02
## 84 7.770082e-10 1.701760e-05 2.466014 -1.274226361 0.30942526 -8.312033e-05
## 85 1.527491e-06 7.667106e-04 2.603707 0.827115575 0.47711259 -2.030021e-03
## 86 4.212838e-03 5.252825e-02 3.998721 1.162830155 0.30956201 -8.477876e-02
## 87 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 88 1.694751e-09 5.460351e-05 2.886602 0.556151184 0.61829812 -1.473314e-04
## 89 4.663488e-07 7.724162e-04 3.253752 0.978495495 0.39485399 -1.597394e-03
## 90 1.302917e-11 2.085634e-06 2.006274 -1.094503029 0.38765266 -1.122965e-05
## 91 2.989827e-10 1.073957e-05 2.614037 -1.045810902 0.38257916 -4.844970e-05
## 92 1.350820e-06 9.577779e-04 3.998660 0.526190858 0.62658949 -2.155595e-03
## 93 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 94 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 95 4.369366e-11 3.817246e-06 2.001871 -0.994758184 0.42458884 -2.020682e-05
## 96 4.242696e-04 1.733560e-02 3.986209 0.808588072 0.46424609 -3.417973e-02
## 97 2.591167e-06 1.421806e-03 3.917100 0.721281731 0.51143571 -2.955210e-03
## 98 2.015533e-03 3.083267e-02 3.416089 0.098387614 0.92711989 -8.866305e-02
## 99 4.173386e-04 1.448185e-02 3.614395 0.223120302 0.83547508 -3.872645e-02
## 100 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 101 4.211103e-08 1.311245e-04 2.856219 -1.285251647 0.29300810 -5.979642e-04
## 102 1.588944e-04 1.131531e-02 3.884206 -0.467331522 0.66527107 -3.707718e-02
## 103 2.341621e-08 2.190577e-04 2.748771 1.164430680 0.33529245 -4.795310e-04
## 104 2.687326e-14 1.255765e-07 3.927266 -1.714451549 0.16292032 -5.665125e-07
## 105 5.127956e-14 9.494906e-07 2.077277 0.862414808 0.47642347 -3.124234e-06
## 106 3.178354e-11 3.285451e-06 2.075362 -1.469552965 0.27515357 -1.848360e-05
## 107 6.090755e-07 5.352398e-04 3.406582 0.218409250 0.83955401 -1.477040e-03
## 108 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 109 1.346335e-12 2.678004e-06 2.265829 1.447249444 0.27079472 -6.443529e-06
## 110 8.766890e-03 8.095776e-02 3.953661 0.076662116 0.94261473 -2.196112e-01
## 111 1.430490e-05 3.926859e-03 3.491674 -0.244946405 0.82020525 -1.252001e-02
## 112 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 113 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 114 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 115 2.712071e-04 1.179994e-02 3.672702 0.093946196 0.93005068 -3.283753e-02
## 116 3.317208e-02 1.585004e-01 3.943479 -0.110639737 0.91730374 -4.601028e-01
## 117 9.504537e-10 2.085043e-05 3.307672 -0.240087568 0.82438486 -6.800259e-05
## 118 3.916453e-07 6.220242e-04 3.617478 -0.271023179 0.80112416 -1.970060e-03
## 119 1.020268e-04 6.595225e-03 3.035363 -1.012360077 0.38513654 -2.752803e-02
## 120 1.213451e-05 2.011178e-03 2.000000 -1.000099799 0.42261132 -1.066478e-02
## 121 6.437810e-01 6.062093e-01 3.890344 -0.854981521 0.44201528 -2.220281e+00
## 122 1.370274e-06 1.039740e-03 3.906177 0.446985945 0.67854601 -2.449585e-03
## 123 6.513407e-07 2.477558e-03 2.146472 -1.728419188 0.21744638 -1.427471e-02
## 124 3.631821e-06 1.173341e-03 2.538745 -2.281153568 0.12276875 -6.825416e-03
## 125 1.282196e-04 9.216315e-03 3.999839 -0.378853950 0.72404842 -2.908064e-02
## 126 8.131058e-06 2.386933e-03 3.990585 -0.687366586 0.52971661 -8.274057e-03
## 127 6.697264e-07 6.203375e-04 3.899857 -0.536501136 0.62075005 -2.072728e-03
## 128 8.859344e-07 8.361301e-04 3.905938 -0.018968325 0.98579561 -2.359544e-03
## 129 1.301434e-05 2.924620e-03 3.999175 0.373612028 0.72765547 -7.028034e-03
## 130 6.807176e-11 9.554499e-06 3.192619 1.420384307 0.24536874 -1.582369e-05
## 131 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 132 5.190383e-13 4.240571e-07 2.157241 -0.932533643 0.44328028 -2.098315e-06
## 133 1.289085e-08 8.806442e-05 3.953775 -0.547250880 0.61362787 -2.938309e-04
## 134 7.239757e-02 2.385119e-01 3.910162 0.960105303 0.39253324 -4.392625e-01
## 135 2.004818e-08 8.563848e-05 2.386129 -0.002812219 0.99797408 -3.170784e-04
## 136 3.893901e-06 1.852320e-03 3.776265 -0.419383930 0.69768579 -6.042125e-03
## 137 1.234653e-07 2.963572e-04 3.984276 -0.177632729 0.86767642 -8.767444e-04
## 138 1.166248e-10 7.722592e-06 3.662246 -0.444243567 0.68184004 -2.567491e-05
## 139 6.999173e-08 1.997803e-04 3.888805 -0.401198762 0.70933791 -6.411418e-04
## 140 1.676114e-06 7.631918e-04 2.169779 -1.292108620 0.31678857 -4.035531e-03
## 141 2.619467e-02 1.068326e-01 3.122599 -0.219986136 0.83948423 -3.560625e-01
## 142 1.021160e-05 2.687299e-03 3.986907 -2.898406983 0.04436349 -1.525970e-02
## 143 2.820365e-04 1.188635e-02 3.605429 -0.532264644 0.62563340 -4.080281e-02
## 144 9.846462e-03 7.998323e-02 3.997276 -0.433787777 0.68682802 -2.568245e-01
## 145 4.899676e-12 1.658165e-06 3.863435 2.114262463 0.10444122 -1.162904e-06
## 146 1.314730e-05 2.095612e-03 2.008357 -0.251563085 0.82478066 -9.508009e-03
## 147 5.413918e-07 7.133216e-04 3.688377 -0.250276811 0.81568781 -2.226795e-03
## 148 3.741917e-07 4.713306e-04 3.940456 -0.536882277 0.62022649 -1.569508e-03
## 149 1.837944e-07 3.099413e-04 3.717807 -0.309309460 0.77363733 -9.827849e-04
## 150 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 151 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 152 3.044154e-06 1.308170e-03 3.866387 -1.051498009 0.35425526 -5.057642e-03
## 153 2.499161e-09 3.337849e-05 3.211658 -1.047575441 0.36718194 -1.373391e-04
## 154 5.465821e-08 1.786397e-04 3.921105 -0.983336533 0.38215532 -6.756070e-04
## 155 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 156 3.030700e-04 1.461344e-02 3.988422 -0.514211749 0.63428311 -4.813429e-02
## 157 2.074418e-11 4.042080e-06 3.907848 0.169944251 0.87348771 -1.064083e-05
## 158 8.339528e-07 5.693545e-04 2.646656 -0.348311613 0.75343570 -2.155096e-03
## 159 5.495451e-08 2.199553e-04 3.777418 -0.112306158 0.91629324 -6.498530e-04
## 160 5.127956e-14 1.194923e-06 2.048458 0.968274580 0.43297941 -3.869515e-06
## 161 2.473743e-08 1.051511e-04 3.221615 0.423950052 0.69833280 -2.774058e-04
## 162 1.874722e-06 9.738630e-04 3.633070 -0.781259539 0.48240860 -3.575897e-03
## 163 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 164 2.226326e-09 4.071489e-05 3.956665 -0.447272511 0.67806994 -1.317433e-04
## 165 7.656124e-06 2.256653e-03 3.999979 -0.386415801 0.71886386 -7.137491e-03
## 166 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 167 7.599952e-07 5.955459e-04 3.379414 0.795948448 0.47819816 -1.306396e-03
## 168 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 169 8.500223e-06 1.968485e-03 3.295325 1.555427980 0.20962291 -2.896820e-03
## 170 9.367705e-09 6.898064e-05 3.644202 0.014953045 0.98885189 -1.980943e-04
## 171 1.641840e-08 7.916458e-05 2.568519 -2.546370269 0.09823630 -4.792398e-04
## 172 8.784154e-09 5.923453e-05 2.763244 1.087693958 0.36240076 -1.335567e-04
## 173 5.060586e-10 1.514667e-05 3.274922 0.380546581 0.72689869 -4.022914e-05
## 174 5.703428e-10 1.789914e-05 3.865118 -0.536979701 0.62069648 -5.999893e-05
## 175 3.303537e-05 6.094833e-03 3.431269 -0.240898855 0.82332616 -1.955590e-02
## 176 6.231524e-05 5.354286e-03 3.328631 -2.084666689 0.11941719 -2.728834e-02
## 177 3.866196e-10 1.148964e-05 2.097358 -1.493790847 0.26826422 -6.446379e-05
## 178 1.128616e-10 1.205149e-05 3.246031 0.528964446 0.63088603 -3.038520e-05
## 179 3.708351e-05 5.116440e-03 3.987670 -0.317623409 0.76670678 -1.584796e-02
## 180 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 181 1.046260e-10 5.972716e-06 2.091477 1.293920922 0.32013104 -1.692267e-05
## 182 3.001176e-11 3.229217e-06 2.169199 -0.792699281 0.50537438 -1.546541e-05
## 183 1.756177e-05 2.952489e-03 3.578774 -0.156522679 0.88404652 -9.054458e-03
## 184 2.466702e-10 1.361319e-05 3.949895 -0.423079799 0.69425395 -4.374558e-05
## 185 7.984326e-05 6.638312e-03 3.834270 -0.657484141 0.54823822 -2.311396e-02
## 186 3.375958e-05 5.386690e-03 3.808305 2.201891824 0.09586980 -3.396567e-03
## 187 1.849761e-04 1.136425e-02 3.991868 -0.649051232 0.55175675 -3.895357e-02
## 188 7.814572e-10 2.746230e-05 3.650912 0.211705670 0.84362199 -7.339678e-05
## 189 2.947360e-09 4.058448e-05 3.856428 -0.487884034 0.65206503 -1.341550e-04
## 190 5.632449e-10 1.465315e-05 2.562911 -1.014566166 0.39633988 -6.633798e-05
## 191 8.705644e-05 8.041430e-03 3.958426 -0.638031836 0.55849242 -2.755006e-02
## 192 7.941257e-10 2.002515e-05 3.627984 -0.264878801 0.80546053 -6.322521e-05
## 193 5.127956e-14 1.114050e-05 2.000551 0.997204950 0.42370231 -3.681171e-05
## 194 1.018537e-08 1.059289e-04 3.460466 -0.100145492 0.92575576 -3.237120e-04
## 195 2.803760e-07 3.654921e-04 3.450072 -0.914447453 0.41981958 -1.416080e-03
## 196 3.622569e-05 4.535107e-03 3.882163 -0.564747599 0.60327609 -1.530484e-02
## 197 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 198 5.932964e-11 1.052451e-05 2.830187 0.972769377 0.40629055 -2.442187e-05
## 199 6.132144e-10 1.840278e-05 3.835448 0.610172529 0.57604301 -4.074168e-05
## 200 1.883824e-10 1.046401e-05 3.915425 0.396884439 0.71213214 -2.514893e-05
## 201 8.223634e-07 8.072483e-04 3.901749 0.792738418 0.47334192 -1.623778e-03
## 202 1.210478e-12 1.455412e-06 2.891855 -3.380976350 0.04549459 -9.652034e-06
## 203 5.433706e-05 6.246408e-03 3.979609 0.967367283 0.38840173 -1.133534e-02
## 204 1.149388e-07 2.655733e-04 3.970333 -0.310424918 0.77185008 -8.219682e-04
## 205 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 206 1.767646e-09 4.663711e-05 3.305935 1.133078549 0.33261139 -8.810104e-05
## 207 5.951698e-09 8.033304e-05 3.483261 0.945997651 0.40503703 -1.607207e-04
## 208 8.670426e-12 5.483354e-06 2.420623 0.637546281 0.57886333 -1.657281e-05
## 209 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 210 5.127956e-14 1.823009e-04 2.000002 0.999056530 0.42301295 -6.022476e-04
## 211 1.416811e-07 2.961229e-04 3.976332 -0.617984488 0.57022238 -1.007102e-03
## 212 1.490295e-11 2.421965e-06 2.700392 -1.081972158 0.36631213 -1.083539e-05
## 213 1.550207e-06 7.870197e-04 2.764535 -0.805775430 0.48386750 -3.263928e-03
## 214 6.439303e-08 1.992405e-04 3.973661 -0.042485726 0.96816052 -5.630943e-04
## 215 4.621689e-07 4.545980e-04 3.223200 -0.093393127 0.93109758 -1.434133e-03
## 216 3.438029e-10 1.114642e-05 2.334164 -1.710516999 0.21108484 -6.101094e-05
## 217 1.278225e-04 6.683235e-03 2.192769 0.696407577 0.55263812 -2.180969e-02
## 218 8.196253e-07 7.544194e-04 3.993629 -0.265981880 0.80342013 -2.296584e-03
## 219 1.461409e-06 8.246181e-04 3.369031 -0.776286644 0.48843884 -3.109085e-03
## 220 9.478272e-05 6.919600e-03 3.629074 -0.941025181 0.40500797 -2.652317e-02
## 221 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 222 3.576995e-08 1.404611e-04 3.833063 -0.449417381 0.67736513 -4.598986e-04
## 223 2.240984e-06 1.223960e-03 3.999970 -0.541728009 0.61677076 -4.061323e-03
## 224 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 225 4.546724e-10 1.305558e-05 2.490951 -2.575685438 0.09904142 -8.041781e-05
## 226 1.218695e-09 2.056824e-05 2.165346 -1.547220467 0.25256715 -1.141510e-04
## 227 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 228 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 229 1.970690e-07 3.770764e-04 3.977024 -0.514615418 0.63409898 -1.243371e-03
## 230 9.556602e-06 2.789092e-03 3.873119 -0.443049954 0.68134611 -9.080549e-03
## 231 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 232 1.420003e-09 2.234284e-05 2.217954 0.436982909 0.70103229 -7.786125e-05
## 233 1.289675e-08 1.797855e-04 2.599499 1.004646573 0.39923593 -4.447879e-04
## 234 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 235 7.085485e-10 1.721562e-05 2.957280 -1.874599888 0.15886200 -8.751067e-05
## 236 1.615499e-10 9.349300e-06 3.795480 3.287642182 0.03272312 4.218284e-06
## 237 1.800461e-10 8.044345e-06 2.311089 -0.080458550 0.94230990 -3.115647e-05
## 238 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 239 1.568550e-08 1.020438e-04 3.999928 -0.990332379 0.37807254 -3.843783e-04
## 240 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 241 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 242 2.770353e-12 1.224121e-06 3.794826 -1.238703476 0.28655550 -4.988740e-06
## 243 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 244 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 245 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 246 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 247 1.285011e-06 7.476631e-04 3.116312 -0.914481187 0.42560246 -3.013662e-03
## 248 2.843140e-08 1.340779e-04 3.988211 0.592017913 0.58576951 -2.933179e-04
## 249 5.127956e-14 1.495742e-07 3.127821 -0.641699416 0.56500822 -5.611756e-07
## 250 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 251 1.990322e-07 3.135296e-04 3.563880 -0.004989194 0.99628575 -9.157265e-04
## 252 5.530387e-14 1.588909e-07 3.300466 -1.176986099 0.31705586 -6.676036e-07
## 253 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 254 4.093996e-11 4.827612e-06 3.886239 0.196011348 0.85442200 -1.261350e-05
## 255 7.637616e-09 7.016301e-05 3.995297 0.640236231 0.55689126 -1.499733e-04
## 256 1.062131e-10 5.950729e-06 2.000770 -1.024837835 0.41317232 -3.169302e-05
## 257 5.310362e-09 5.834192e-05 3.993581 0.098604843 0.92620304 -1.563331e-04
## 258 3.166836e-11 5.796971e-06 3.514325 0.552384137 0.61390759 -1.380916e-05
## 259 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 260 6.542226e-08 1.498039e-04 2.116153 -0.945259230 0.43956787 -7.534137e-04
## 261 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 262 1.118109e-10 6.794675e-06 2.903409 -0.468461741 0.67237274 -2.521945e-05
## 263 2.042081e-03 4.518992e-02 3.599966 0.871815135 0.43757938 -9.176457e-02
## 264 1.616091e-10 1.180257e-05 3.804691 0.891302480 0.42554061 -2.292372e-05
## 265 2.649850e-10 1.424643e-05 3.933923 0.197186060 0.85344977 -3.700865e-05
## 266 1.940519e-09 3.568091e-05 3.998958 0.574546221 0.59637298 -7.857594e-05
## 267 5.127956e-14 9.123503e-07 2.083826 1.394466518 0.29323675 -2.505775e-06
## 268 1.209090e-09 2.495006e-05 3.680040 0.314888199 0.76988257 -6.385719e-05
## 269 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 270 5.127956e-14 3.113483e-06 2.007066 0.989327210 0.42646556 -1.027089e-05
## 271 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 272 1.070670e-10 6.094257e-06 2.162353 -0.623529409 0.59234694 -2.822227e-05
## 273 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 274 2.447267e-08 9.031922e-05 2.000003 -1.002221378 0.42179552 -4.791315e-04
## 275 1.891223e-11 2.512148e-06 2.004322 -1.053328863 0.40246096 -1.343271e-05
## 276 5.590165e-10 3.012180e-05 2.969072 1.167333908 0.32821052 -6.126619e-05
## 277 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 278 6.463753e-11 7.551423e-06 3.774675 -0.182237836 0.86476060 -2.284520e-05
## 279 3.281216e-06 1.122584e-03 2.594977 -0.888261386 0.44897040 -4.906843e-03
## 280 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 281 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 282 1.566963e-12 2.942069e-06 2.255820 1.258202312 0.32284781 -7.676098e-06
## 283 3.002835e-12 5.579249e-06 2.132750 0.778959233 0.51310161 -1.828304e-05
## 284 1.132912e-07 3.066181e-04 3.851086 0.349956543 0.74466754 -7.571462e-04
## 285 6.418995e-09 6.553525e-05 3.999948 -1.640996695 0.17614305 -2.894991e-04
## 286 2.545477e-05 3.711461e-03 3.795807 -0.895840950 0.42350889 -1.385189e-02
## 287 8.902494e-10 2.359349e-05 3.982550 -0.778376219 0.48000041 -8.398399e-05
## 288 6.652175e-12 2.320919e-06 3.878875 -0.913389160 0.41421376 -8.643946e-06
## 289 6.695888e-13 1.342415e-06 2.554376 0.095972135 0.93056233 -4.597534e-06
## 290 6.140709e-14 5.319706e-07 2.309996 0.611206277 0.59583139 -1.693181e-06
## 291 9.744566e-13 7.004840e-07 3.620074 -0.706009167 0.52294920 -2.522608e-06
## 292 5.127956e-14 1.360808e-06 2.037263 1.030348796 0.40944896 -4.351536e-06
## 293 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 294 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 295 4.416463e-11 7.741745e-06 3.177571 0.487303946 0.65769643 -2.010423e-05
## 296 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 297 6.735587e-13 8.221899e-07 3.594796 2.482141933 0.07513168 -3.471224e-07
## 298 1.469096e-12 2.104284e-05 2.004429 1.221630114 0.34606571 -6.464204e-05
## 299 5.394076e-12 2.710918e-06 3.172604 2.902675027 0.05827768 -4.988903e-07
## 300 1.096336e-10 1.036723e-05 3.628506 -0.222107167 0.83616270 -3.228701e-05
## 301 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 302 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 303 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 304 5.127956e-14 1.049366e-06 2.063054 2.005179377 0.17889054 -2.281193e-06
## 305 1.364312e-03 2.608707e-02 3.593120 -0.997820684 0.38073342 -1.018117e-01
## 306 1.591811e-10 1.059529e-05 3.988072 1.119099751 0.32593316 -1.759479e-05
## 307 4.297583e-10 2.124592e-05 3.529111 0.253313725 0.81408421 -5.684384e-05
## 308 5.866319e-10 1.453203e-05 2.317810 0.349191475 0.75616831 -4.991368e-05
## 309 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 310 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 311 1.057412e-09 1.980568e-05 2.445921 -0.244502976 0.82586891 -7.677361e-05
## 312 2.013439e-06 1.056067e-03 3.840862 0.131452920 0.90201318 -2.841827e-03
## 313 2.616823e-10 9.873443e-06 2.463961 -0.318564793 0.77509189 -3.881157e-05
## 314 2.177853e-07 3.582607e-04 3.932312 -0.887298917 0.42585326 -1.319364e-03
## 315 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 316 7.289965e-10 1.922006e-05 3.637669 0.637765814 0.56154144 -4.326858e-05
## 317 2.427935e-10 3.484244e-05 2.284257 0.712164191 0.54209021 -1.085693e-04
## 318 5.127956e-14 9.535345e-06 2.000752 1.439468665 0.28661941 -2.728667e-05
## 319 6.009789e-09 9.055538e-05 3.170713 1.096319985 0.34914707 -1.803279e-04
## 320 1.335605e-11 2.119532e-06 2.036296 -0.880360053 0.47009461 -1.083155e-05
## 321 1.669162e-07 2.466130e-04 2.369148 -1.240053263 0.32364058 -1.223200e-03
## 322 2.060039e-10 1.193330e-05 3.994941 0.517624913 0.63206230 -2.697173e-05
## 323 1.035491e-09 2.388375e-05 3.830772 0.858724343 0.44089878 -4.697379e-05
## 324 1.003929e-10 1.154944e-05 3.204484 -0.570347015 0.60599788 -4.205079e-05
## 325 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 326 6.673158e-08 1.729367e-04 3.231838 0.897543500 0.43122966 -3.734689e-04
## 327 1.386362e-13 2.302689e-07 2.577066 -1.717611919 0.19914928 -1.201298e-06
## 328 1.003085e-06 6.793644e-04 3.329122 -0.976425530 0.39435357 -2.709360e-03
## 329 8.364192e-09 5.922462e-05 2.967794 -1.397378007 0.25763769 -2.724007e-04
## 330 6.449703e-07 6.268611e-04 3.964801 -0.809463056 0.46402714 -2.253977e-03
## 331 4.243041e-07 6.169965e-04 3.752271 -0.283659724 0.79162766 -1.933613e-03
## 332 3.018261e-07 4.596297e-04 3.990982 0.627597201 0.56438681 -9.888124e-04
## 333 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 334 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 335 1.148728e-06 7.571292e-04 3.594363 0.031339048 0.97666069 -2.175348e-03
## 336 4.697031e-06 1.618066e-03 3.851987 -1.004790404 0.37387432 -6.187194e-03
## 337 2.747183e-08 1.611093e-04 3.680954 -0.759487321 0.49327834 -5.853848e-04
## 338 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 339 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 340 1.061301e-12 6.004821e-07 2.076991 -1.198430473 0.34960818 -3.213660e-06
## 341 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 342 5.127956e-14 1.147872e-06 2.052564 1.805751321 0.20947046 -2.746876e-06
## 343 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 344 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 345 2.379490e-11 6.246604e-06 2.958157 0.329013089 0.76405994 -1.798430e-05
## 346 1.598883e-06 8.164316e-04 2.943414 0.527792335 0.63483843 -2.195831e-03
## 347 2.452090e-09 4.539991e-05 3.835819 -0.410961754 0.70301673 -1.468645e-04
## 348 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 349 1.556897e-02 7.325340e-02 2.135811 -0.091346303 0.93506989 -3.034275e-01
## 350 1.555036e-07 3.139626e-04 3.989335 -0.574772698 0.59630383 -1.053077e-03
## 351 1.387138e-06 1.017891e-03 3.954334 0.478194900 0.65774611 -2.352286e-03
## 352 2.225967e-11 5.348306e-06 3.247916 1.172688985 0.31969818 -1.003690e-05
## 353 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 354 5.127956e-14 1.546121e-07 3.375569 -0.690544905 0.53436191 -5.692448e-07
## 355 5.028783e-04 1.861157e-02 3.995868 0.727082761 0.50748478 -3.816293e-02
## 356 1.019960e-04 9.444380e-03 3.786139 1.063840830 0.35048936 -1.676908e-02
## 357 4.323969e-10 1.258304e-05 2.390312 -2.005334274 0.16155259 -7.172494e-05
## 358 1.995379e-12 8.197182e-07 2.040961 -0.970838483 0.43227379 -4.255806e-06
## 359 7.096196e-03 1.142942e-01 2.843220 -1.044526302 0.37679808 -4.947328e-01
## 360 4.196268e-05 4.107068e-03 2.790205 1.180872161 0.32846293 -8.794551e-03
## conf.high mean.null alternative conf.level
## 1 3.557117e-07 0 two.sided 0.95
## 2 1.347725e-02 0 two.sided 0.95
## 3 6.634885e-02 0 two.sided 0.95
## 4 6.803589e-03 0 two.sided 0.95
## 5 1.650329e-03 0 two.sided 0.95
## 6 7.708100e-02 0 two.sided 0.95
## 7 1.908278e-03 0 two.sided 0.95
## 8 4.437648e-03 0 two.sided 0.95
## 9 1.421473e-05 0 two.sided 0.95
## 10 3.237282e-01 0 two.sided 0.95
## 11 6.912629e-05 0 two.sided 0.95
## 12 3.755233e-03 0 two.sided 0.95
## 13 1.447974e-04 0 two.sided 0.95
## 14 1.042589e-01 0 two.sided 0.95
## 15 3.291295e-03 0 two.sided 0.95
## 16 1.363408e-04 0 two.sided 0.95
## 17 2.481507e-03 0 two.sided 0.95
## 18 1.218176e-02 0 two.sided 0.95
## 19 1.957327e-04 0 two.sided 0.95
## 20 1.369350e-04 0 two.sided 0.95
## 21 2.045286e-02 0 two.sided 0.95
## 22 1.505624e-01 0 two.sided 0.95
## 23 6.414202e-03 0 two.sided 0.95
## 24 4.201405e-02 0 two.sided 0.95
## 25 4.714506e-06 0 two.sided 0.95
## 26 3.299671e-04 0 two.sided 0.95
## 27 2.708041e-04 0 two.sided 0.95
## 28 2.304214e-03 0 two.sided 0.95
## 29 1.135894e-03 0 two.sided 0.95
## 30 3.535049e-03 0 two.sided 0.95
## 31 7.908572e-05 0 two.sided 0.95
## 32 1.530476e-05 0 two.sided 0.95
## 33 1.154508e-05 0 two.sided 0.95
## 34 2.319395e-04 0 two.sided 0.95
## 35 3.557117e-07 0 two.sided 0.95
## 36 2.987206e-02 0 two.sided 0.95
## 37 1.538628e-03 0 two.sided 0.95
## 38 1.139554e-03 0 two.sided 0.95
## 39 9.605537e-04 0 two.sided 0.95
## 40 8.212326e-06 0 two.sided 0.95
## 41 2.207794e-05 0 two.sided 0.95
## 42 3.557117e-07 0 two.sided 0.95
## 43 2.482004e-02 0 two.sided 0.95
## 44 3.334999e-01 0 two.sided 0.95
## 45 5.831251e-02 0 two.sided 0.95
## 46 1.192818e-03 0 two.sided 0.95
## 47 4.554625e-05 0 two.sided 0.95
## 48 3.557117e-07 0 two.sided 0.95
## 49 3.557117e-07 0 two.sided 0.95
## 50 -2.040050e-06 0 two.sided 0.95
## 51 1.558016e-03 0 two.sided 0.95
## 52 9.696613e-03 0 two.sided 0.95
## 53 3.733341e-05 0 two.sided 0.95
## 54 8.182397e-03 0 two.sided 0.95
## 55 4.643133e-05 0 two.sided 0.95
## 56 1.890341e-02 0 two.sided 0.95
## 57 8.550789e-04 0 two.sided 0.95
## 58 2.230769e-02 0 two.sided 0.95
## 59 4.068607e-02 0 two.sided 0.95
## 60 1.151177e-01 0 two.sided 0.95
## 61 5.784980e-03 0 two.sided 0.95
## 62 4.124028e-04 0 two.sided 0.95
## 63 3.691953e-05 0 two.sided 0.95
## 64 2.721389e-05 0 two.sided 0.95
## 65 8.869198e-04 0 two.sided 0.95
## 66 3.000589e-04 0 two.sided 0.95
## 67 3.730250e-04 0 two.sided 0.95
## 68 3.557117e-07 0 two.sided 0.95
## 69 6.029460e-04 0 two.sided 0.95
## 70 9.223744e-04 0 two.sided 0.95
## 71 3.493613e-05 0 two.sided 0.95
## 72 1.981578e-01 0 two.sided 0.95
## 73 1.959661e-04 0 two.sided 0.95
## 74 8.579580e-06 0 two.sided 0.95
## 75 4.620487e-03 0 two.sided 0.95
## 76 4.954847e-02 0 two.sided 0.95
## 77 1.882278e-04 0 two.sided 0.95
## 78 2.939473e-03 0 two.sided 0.95
## 79 3.672748e-05 0 two.sided 0.95
## 80 2.022011e-03 0 two.sided 0.95
## 81 1.394718e-05 0 two.sided 0.95
## 82 1.820288e-04 0 two.sided 0.95
## 83 5.410335e-02 0 two.sided 0.95
## 84 3.975179e-05 0 two.sided 0.95
## 85 3.298338e-03 0 two.sided 0.95
## 86 2.069416e-01 0 two.sided 0.95
## 87 3.557117e-07 0 two.sided 0.95
## 88 2.080670e-04 0 two.sided 0.95
## 89 3.109006e-03 0 two.sided 0.95
## 90 6.664187e-06 0 two.sided 0.95
## 91 2.598658e-05 0 two.sided 0.95
## 92 3.163543e-03 0 two.sided 0.95
## 93 3.557117e-07 0 two.sided 0.95
## 94 3.557117e-07 0 two.sided 0.95
## 95 1.261235e-05 0 two.sided 0.95
## 96 6.221446e-02 0 two.sided 0.95
## 97 5.006255e-03 0 two.sided 0.95
## 98 9.473015e-02 0 two.sided 0.95
## 99 4.518884e-02 0 two.sided 0.95
## 100 3.557117e-07 0 two.sided 0.95
## 101 2.609081e-04 0 two.sided 0.95
## 102 2.650118e-02 0 two.sided 0.95
## 103 9.896860e-04 0 two.sided 0.95
## 104 1.359228e-07 0 two.sided 0.95
## 105 4.761943e-06 0 two.sided 0.95
## 106 8.827315e-06 0 two.sided 0.95
## 107 1.710843e-03 0 two.sided 0.95
## 108 3.557117e-07 0 two.sided 0.95
## 109 1.419501e-05 0 two.sided 0.95
## 110 2.320240e-01 0 two.sided 0.95
## 111 1.059627e-02 0 two.sided 0.95
## 112 3.557117e-07 0 two.sided 0.95
## 113 3.557117e-07 0 two.sided 0.95
## 114 3.557117e-07 0 two.sided 0.95
## 115 3.505465e-02 0 two.sided 0.95
## 116 4.250299e-01 0 two.sided 0.95
## 117 5.799074e-05 0 two.sided 0.95
## 118 1.632895e-03 0 two.sided 0.95
## 119 1.417455e-02 0 two.sided 0.95
## 120 6.642023e-03 0 two.sided 0.95
## 121 1.183685e+00 0 two.sided 0.95
## 122 3.379084e-03 0 two.sided 0.95
## 123 5.710189e-03 0 two.sided 0.95
## 124 1.472274e-03 0 two.sided 0.95
## 125 2.209736e-02 0 two.sided 0.95
## 126 4.992661e-03 0 two.sided 0.95
## 127 1.407105e-03 0 two.sided 0.95
## 128 2.327824e-03 0 two.sided 0.95
## 129 9.213380e-03 0 two.sided 0.95
## 130 4.296581e-05 0 two.sided 0.95
## 131 3.557117e-07 0 two.sided 0.95
## 132 1.307420e-06 0 two.sided 0.95
## 133 1.974442e-04 0 two.sided 0.95
## 134 8.972556e-01 0 two.sided 0.95
## 135 3.165967e-04 0 two.sided 0.95
## 136 4.488459e-03 0 two.sided 0.95
## 137 7.714589e-04 0 two.sided 0.95
## 138 1.881348e-05 0 two.sided 0.95
## 139 4.808386e-04 0 two.sided 0.95
## 140 2.063278e-03 0 two.sided 0.95
## 141 3.090591e-01 0 two.sided 0.95
## 142 -3.180747e-04 0 two.sided 0.95
## 143 2.814945e-02 0 two.sided 0.95
## 144 1.874330e-01 0 two.sided 0.95
## 145 8.174497e-06 0 two.sided 0.95
## 146 8.453652e-03 0 two.sided 0.95
## 147 1.869739e-03 0 two.sided 0.95
## 148 1.063410e-03 0 two.sided 0.95
## 149 7.910493e-04 0 two.sided 0.95
## 150 3.557117e-07 0 two.sided 0.95
## 151 3.557117e-07 0 two.sided 0.95
## 152 2.306565e-03 0 two.sided 0.95
## 153 6.740615e-05 0 two.sided 0.95
## 154 3.242810e-04 0 two.sided 0.95
## 155 3.557117e-07 0 two.sided 0.95
## 156 3.310549e-02 0 two.sided 0.95
## 157 1.201469e-05 0 two.sided 0.95
## 158 1.758471e-03 0 two.sided 0.95
## 159 6.004484e-04 0 two.sided 0.95
## 160 6.183543e-06 0 two.sided 0.95
## 161 3.665634e-04 0 two.sided 0.95
## 162 2.054218e-03 0 two.sided 0.95
## 163 3.557117e-07 0 two.sided 0.95
## 164 9.532196e-05 0 two.sided 0.95
## 165 5.393479e-03 0 two.sided 0.95
## 166 3.557117e-07 0 two.sided 0.95
## 167 2.254444e-03 0 two.sided 0.95
## 168 3.557117e-07 0 two.sided 0.95
## 169 9.020493e-03 0 two.sided 0.95
## 170 2.001572e-04 0 two.sided 0.95
## 171 7.607507e-05 0 two.sided 0.95
## 172 2.624148e-04 0 two.sided 0.95
## 173 5.175717e-05 0 two.sided 0.95
## 174 4.077598e-05 0 two.sided 0.95
## 175 1.661942e-02 0 two.sided 0.95
## 176 4.964537e-03 0 two.sided 0.95
## 177 3.013756e-05 0 two.sided 0.95
## 178 4.313483e-05 0 two.sided 0.95
## 179 1.259775e-02 0 two.sided 0.95
## 180 3.557117e-07 0 two.sided 0.95
## 181 3.237911e-05 0 two.sided 0.95
## 182 1.034581e-05 0 two.sided 0.95
## 183 8.130195e-03 0 two.sided 0.95
## 184 3.222665e-05 0 two.sided 0.95
## 185 1.438480e-02 0 two.sided 0.95
## 186 2.711838e-02 0 two.sided 0.95
## 187 2.420160e-02 0 two.sided 0.95
## 188 8.502463e-05 0 two.sided 0.95
## 189 9.455394e-05 0 two.sided 0.95
## 190 3.660481e-05 0 two.sided 0.95
## 191 1.728868e-02 0 two.sided 0.95
## 192 5.261673e-05 0 two.sided 0.95
## 193 5.903044e-05 0 two.sided 0.95
## 194 3.024954e-04 0 two.sided 0.95
## 195 7.476331e-04 0 two.sided 0.95
## 196 1.018246e-02 0 two.sided 0.95
## 197 3.557117e-07 0 two.sided 0.95
## 198 4.489771e-05 0 two.sided 0.95
## 199 6.319942e-05 0 two.sided 0.95
## 200 3.345494e-05 0 two.sided 0.95
## 201 2.903652e-03 0 two.sided 0.95
## 202 -1.893952e-07 0 two.sided 0.95
## 203 2.342048e-02 0 two.sided 0.95
## 204 6.570871e-04 0 two.sided 0.95
## 205 3.557117e-07 0 two.sided 0.95
## 206 1.937881e-04 0 two.sided 0.95
## 207 3.127104e-04 0 two.sided 0.95
## 208 2.356459e-05 0 two.sided 0.95
## 209 3.557117e-07 0 two.sided 0.95
## 210 9.665054e-04 0 two.sided 0.95
## 211 6.411033e-04 0 two.sided 0.95
## 212 5.594395e-06 0 two.sided 0.95
## 213 1.995606e-03 0 two.sided 0.95
## 214 5.461646e-04 0 two.sided 0.95
## 215 1.349220e-03 0 two.sided 0.95
## 216 2.287867e-05 0 two.sided 0.95
## 217 3.111820e-02 0 two.sided 0.95
## 218 1.895261e-03 0 two.sided 0.95
## 219 1.828805e-03 0 two.sided 0.95
## 220 1.350013e-02 0 two.sided 0.95
## 221 3.557117e-07 0 two.sided 0.95
## 222 3.336473e-04 0 two.sided 0.95
## 223 2.735215e-03 0 two.sided 0.95
## 224 3.557117e-07 0 two.sided 0.95
## 225 1.316366e-05 0 two.sided 0.95
## 226 5.050376e-05 0 two.sided 0.95
## 227 3.557117e-07 0 two.sided 0.95
## 228 3.557117e-07 0 two.sided 0.95
## 229 8.552722e-04 0 two.sided 0.95
## 230 6.609134e-03 0 two.sided 0.95
## 231 3.557117e-07 0 two.sided 0.95
## 232 9.738813e-05 0 two.sided 0.95
## 233 8.060298e-04 0 two.sided 0.95
## 234 3.557117e-07 0 two.sided 0.95
## 235 2.296586e-05 0 two.sided 0.95
## 236 5.725602e-05 0 two.sided 0.95
## 237 2.986199e-05 0 two.sided 0.95
## 238 3.557117e-07 0 two.sided 0.95
## 239 1.822638e-04 0 two.sided 0.95
## 240 3.557117e-07 0 two.sided 0.95
## 241 3.557117e-07 0 two.sided 0.95
## 242 1.956094e-06 0 two.sided 0.95
## 243 3.557117e-07 0 two.sided 0.95
## 244 3.557117e-07 0 two.sided 0.95
## 245 3.557117e-07 0 two.sided 0.95
## 246 3.557117e-07 0 two.sided 0.95
## 247 1.646214e-03 0 two.sided 0.95
## 248 4.520710e-04 0 two.sided 0.95
## 249 3.692123e-07 0 two.sided 0.95
## 250 3.557117e-07 0 two.sided 0.95
## 251 9.125979e-04 0 two.sided 0.95
## 252 2.935787e-07 0 two.sided 0.95
## 253 3.557117e-07 0 two.sided 0.95
## 254 1.450603e-05 0 two.sided 0.95
## 255 2.398151e-04 0 two.sided 0.95
## 256 1.949595e-05 0 two.sided 0.95
## 257 1.678387e-04 0 two.sided 0.95
## 258 2.021347e-05 0 two.sided 0.95
## 259 3.557117e-07 0 two.sided 0.95
## 260 4.702067e-04 0 two.sided 0.95
## 261 3.557117e-07 0 two.sided 0.95
## 262 1.885336e-05 0 two.sided 0.95
## 263 1.705591e-01 0 two.sided 0.95
## 264 4.396304e-05 0 two.sided 0.95
## 265 4.262704e-05 0 two.sided 0.95
## 266 1.195766e-04 0 two.sided 0.95
## 267 5.050259e-06 0 two.sided 0.95
## 268 7.957014e-05 0 two.sided 0.95
## 269 3.557117e-07 0 two.sided 0.95
## 270 1.643140e-05 0 two.sided 0.95
## 271 3.557117e-07 0 two.sided 0.95
## 272 2.062237e-05 0 two.sided 0.95
## 273 3.557117e-07 0 two.sided 0.95
## 274 2.980918e-04 0 two.sided 0.95
## 275 8.140478e-06 0 two.sided 0.95
## 276 1.315906e-04 0 two.sided 0.95
## 277 3.557117e-07 0 two.sided 0.95
## 278 2.009289e-05 0 two.sided 0.95
## 279 2.912547e-03 0 two.sided 0.95
## 280 3.557117e-07 0 two.sided 0.95
## 281 3.557117e-07 0 two.sided 0.95
## 282 1.507954e-05 0 two.sided 0.95
## 283 2.697505e-05 0 two.sided 0.95
## 284 9.717523e-04 0 two.sided 0.95
## 285 7.441283e-05 0 two.sided 0.95
## 286 7.202132e-03 0 two.sided 0.95
## 287 4.725477e-05 0 two.sided 0.95
## 288 4.404142e-06 0 two.sided 0.95
## 289 4.855203e-06 0 two.sided 0.95
## 290 2.343469e-06 0 two.sided 0.95
## 291 1.533511e-06 0 two.sided 0.95
## 292 7.155749e-06 0 two.sided 0.95
## 293 3.557117e-07 0 two.sided 0.95
## 294 3.557117e-07 0 two.sided 0.95
## 295 2.764939e-05 0 two.sided 0.95
## 296 3.557117e-07 0 two.sided 0.95
## 297 4.428707e-06 0 two.sided 0.95
## 298 1.160552e-04 0 two.sided 0.95
## 299 1.623672e-05 0 two.sided 0.95
## 300 2.768174e-05 0 two.sided 0.95
## 301 3.557117e-07 0 two.sided 0.95
## 302 3.557117e-07 0 two.sided 0.95
## 303 3.557117e-07 0 two.sided 0.95
## 304 6.489527e-06 0 two.sided 0.95
## 305 4.975127e-02 0 two.sided 0.95
## 306 4.130916e-05 0 two.sided 0.95
## 307 6.760761e-05 0 two.sided 0.95
## 308 6.006260e-05 0 two.sided 0.95
## 309 3.557117e-07 0 two.sided 0.95
## 310 3.557117e-07 0 two.sided 0.95
## 311 6.708852e-05 0 two.sided 0.95
## 312 3.119473e-03 0 two.sided 0.95
## 313 3.252091e-05 0 two.sided 0.95
## 314 6.835958e-04 0 two.sided 0.95
## 315 3.557117e-07 0 two.sided 0.95
## 316 6.778437e-05 0 two.sided 0.95
## 317 1.581964e-04 0 two.sided 0.95
## 318 5.473833e-05 0 two.sided 0.95
## 319 3.788833e-04 0 two.sided 0.95
## 320 7.099643e-06 0 two.sided 0.95
## 321 6.115739e-04 0 two.sided 0.95
## 322 3.932568e-05 0 two.sided 0.95
## 323 8.799290e-05 0 two.sided 0.95
## 324 2.887641e-05 0 two.sided 0.95
## 325 3.557117e-07 0 two.sided 0.95
## 326 6.839053e-04 0 two.sided 0.95
## 327 4.102724e-07 0 two.sided 0.95
## 328 1.382662e-03 0 two.sided 0.95
## 329 1.068824e-04 0 two.sided 0.95
## 330 1.239135e-03 0 two.sided 0.95
## 331 1.583579e-03 0 two.sided 0.95
## 332 1.565737e-03 0 two.sided 0.95
## 333 3.557117e-07 0 two.sided 0.95
## 334 3.557117e-07 0 two.sided 0.95
## 335 2.222804e-03 0 two.sided 0.95
## 336 2.935560e-03 0 two.sided 0.95
## 337 3.406639e-04 0 two.sided 0.95
## 338 3.557117e-07 0 two.sided 0.95
## 339 3.557117e-07 0 two.sided 0.95
## 340 1.774388e-06 0 two.sided 0.95
## 341 3.557117e-07 0 two.sided 0.95
## 342 6.892419e-06 0 two.sided 0.95
## 343 3.557117e-07 0 two.sided 0.95
## 344 3.557117e-07 0 two.sided 0.95
## 345 2.209473e-05 0 two.sided 0.95
## 346 3.057644e-03 0 two.sided 0.95
## 347 1.095493e-04 0 two.sided 0.95
## 348 3.557117e-07 0 two.sided 0.95
## 349 2.900447e-01 0 two.sided 0.95
## 350 6.921625e-04 0 two.sided 0.95
## 351 3.325787e-03 0 two.sided 0.95
## 352 2.258070e-05 0 two.sided 0.95
## 353 3.557117e-07 0 two.sided 0.95
## 354 3.557117e-07 0 two.sided 0.95
## 355 6.522723e-02 0 two.sided 0.95
## 356 3.686371e-02 0 two.sided 0.95
## 357 2.125853e-05 0 two.sided 0.95
## 358 2.664178e-06 0 two.sided 0.95
## 359 2.559661e-01 0 two.sided 0.95
## 360 1.849439e-02 0 two.sided 0.95Recuperar el valor de P para cada gen de la tabla anterior
Pvalue <- DosDCt %>%
mutate(PV = Tstudent$pvalue,
LPV = -log10(PV)) %>%
select(1,"PV", "LPV")
Pvalue## # A tibble: 360 × 3
## Gen PV LPV
## <chr> <dbl> <dbl>
## 1 ath-miR159a-000338 0.534 0.272
## 2 hsa-let-7a-000377 0.221 0.655
## 3 hsa-let-7b-002619 0.236 0.627
## 4 hsa-let-7c-000379 0.716 0.145
## 5 hsa-let-7d-002283 0.115 0.938
## 6 hsa-let-7e-002406 0.323 0.491
## 7 hsa-let-7f-000382 0.214 0.669
## 8 hsa-let-7g-002282 0.918 0.0370
## 9 hsa-miR-1-002222 0.571 0.244
## 10 hsa-miR-100-000437 0.743 0.129
## # ℹ 350 more rowsObtener el valor de Dos Delta Delta CT
DosDDCT <- DosDCt %>%
mutate(mean_DosDCT_CX = (A1 + A2 + A3) / 3,
mean_DosDCT_TX = (B1 + B2 + B3) / 3,
DosDDCT = mean_DosDCT_TX / mean_DosDCT_CX,
L2DDCT = log2(DosDDCT)) %>% #Para la gráfica necesito el log2 de 2DDCT
select (-2:-7)
DosDDCT## # A tibble: 360 × 5
## Gen mean_DosDCT_CX mean_DosDCT_TX DosDDCT L2DDCT
## <chr> <dbl> <dbl> <dbl> <dbl>
## 1 ath-miR159a-000338 0.00000111 0.00000122 1.10 0.132
## 2 hsa-let-7a-000377 0.0210 0.0339 1.61 0.688
## 3 hsa-let-7b-002619 0.0856 0.146 1.70 0.768
## 4 hsa-let-7c-000379 0.00472 0.00582 1.23 0.303
## 5 hsa-let-7d-002283 0.00802 0.0123 1.53 0.613
## 6 hsa-let-7e-002406 0.123 0.172 1.39 0.476
## 7 hsa-let-7f-000382 0.00142 0.00302 2.13 1.09
## 8 hsa-let-7g-002282 0.00781 0.00798 1.02 0.0320
## 9 hsa-miR-1-002222 0.00000856 0.00000616 0.719 -0.475
## 10 hsa-miR-100-000437 0.392 0.434 1.11 0.150
## # ℹ 350 more rowsGráfica
V_plot <- ggplot(mapping = aes(x= DosDDCT $ L2DDCT,
y= Pvalue $LPV))+
geom_point()
V_plot
Definir valor estadístico de significancia y el factor de cambio
VP <- -log10 (0.05)
FC <- log2 (2)Adicionar los límites de la gráfica
VP2 <- V_plot +
geom_hline(yintercept = VP,
linetype = "dashed",
color="yellow") +
geom_vline(xintercept = FC,
linetype = "dashed",
color= "red") +
geom_vline(xintercept = -FC,
linetype = "dashed",
color= "blue") +
labs(title = "Evaluación de cambio de expresión de miRNAS",
subtitle = "V_plot",
caption = "Creado por: Regina Hernández",
x= "Log2(FC)",
y= "-Log10(valor p)") +
theme_classic()
VP2
Nombres a los valores más añejados y colores a los puntos 1° vamos a definir los valores que cambiaron significativamente
UP_regulated <- DosDDCT %>%
select (1,5) %>%
mutate(LVP = Pvalue$LPV) %>%
filter (LVP > VP,
L2DDCT > FC)
UP_regulated## # A tibble: 2 × 3
## Gen L2DDCT LVP
## <chr> <dbl> <dbl>
## 1 hsa-miR-148a-000470 2.46 1.45
## 2 hsa-miR-429-001024 1.63 1.34Down_regulated <- DosDDCT %>%
select (1,5) %>%
mutate(LVP = Pvalue$LPV) %>%
filter (LVP > VP,
L2DDCT < -FC)
Down_regulated## # A tibble: 0 × 3
## # ℹ 3 variables: Gen <chr>, L2DDCT <dbl>, LVP <dbl>Ordenarlos
top.up <- UP_regulated %>%
arrange(LVP) %>%
head(5)
top.down <- Down_regulated %>%
arrange(LVP) %>%
head(5)
top.up## # A tibble: 2 × 3
## Gen L2DDCT LVP
## <chr> <dbl> <dbl>
## 1 hsa-miR-429-001024 1.63 1.34
## 2 hsa-miR-148a-000470 2.46 1.45top.down## # A tibble: 0 × 3
## # ℹ 3 variables: Gen <chr>, L2DDCT <dbl>, LVP <dbl>Mejorar la gráfica
V_plot3 <- VP2 +
geom_point(data = UP_regulated,
x= UP_regulated$L2DDCT,
y= UP_regulated$LVP,
alpha = 1,
size = 3,
color = "#FF7F50") +
geom_point(data = Down_regulated,
x= Down_regulated$L2DDCT,
y= Down_regulated$LVP,
alpha = 1,
size = 3,
color = "#48D1CC")
V_plot3
Poner etiquetas a los miRNAs sobresalientes
V_plot4 <- V_plot3+
geom_label_repel(data= top.up,
mapping = aes (x = top.up$L2DDCT,
y= top.up $LVP),
label = top.up$Gen,
max.overlaps = 10)+
geom_label_repel(data= top.down,
mapping = aes (x = top.down$L2DDCT,
y= top.down $LVP),
label = top.down$Gen,
max.overlaps = 100)
V_plot4## Warning: Use of `top.up$L2DDCT` is discouraged.
## ℹ Use `L2DDCT` instead.## Warning: Use of `top.up$LVP` is discouraged.
## ℹ Use `LVP` instead.