☆ Objective

This study aims to assess and compare the variance and mean effects of metabolic traits between two progenitor mouse strains: A/J and C57BL/6J on two types of diets(HFHS and control). The metabolic traits include final body weight, BMI, glucose, insulin, HOMA(Homeostatic model assessment, a measurement used to quantify insulin resistance and beta-cell function), Cholesterol, TG (triglycerides, either in plasma or in liver), liver weight, liver TG, and total liver TG. The null hypothesis states that neither of the two strains or diets will differ significantly from each other in terms of any trait, whether considering central tendency or dispersion.

☆ Density Plot for Strains: A/J vs. B6

☆ Skewness & Kurtosis for Strains: A/J vs. B6

☆ Density Plot for Diets: HFHS vs. Control

☆ Skewness & Kurtosis for Diets: HFHS vs. Control

☆ Density Plot for Strains with Diet: AJ_HFHS, AJ_Control, B6_HFHS, B6_Control

☆ Skewness & Kurtosis for Strain with Diet: AJ_HFHS, AJ_Control, B6_HFHS, B6_Control

☆Variance Effect

The table below utilizes four variance methods (Levene’s test, Permutation_Gini_Diff, Permutation_MAD_Diff, and Permutation_SD_Diff) to evaluate the impact of different diets (HFHS and Control) on the variance of metabolic traits across two strains of mice (B6 and AJ). Additionally, it assesses the effects of these two strains on trait variance under each dietary condition. An ensemble voting approach is employed to determine the significance of the results; this approach combines the results from multiple models to make a final decision. If the majority of p-values (from more than two methods) are less than 0.05, we consider the result significant; otherwise, it is considered not significant.

Variance Effect Visualization

-log10(0.05) = 1.3 If more than two methods yield p-values < 0.05, the ensemble voting method will determine the comparison as significant.