##. In this script I will remove Non T cells from PBMC

1. load libraries

2. Load Seurat Object

Cell type Distribution to check clusters

Cell type Distribution barplot

3. filter cells just keep CD4 T cells

Cell type Distribution to check clusters

4. filter B cells from L4

Cell type Distribution to check clusters

5. Save the Seurat object as an Robj file

---
title: "use Annotated Robj including PBMC10x to remove NonCD4Tcells from Control and B cells from L4 and keep just CD4Tcells"
author: Nasir Mahmood Abbasi
date: "`r Sys.Date()`"
output:
  # pdf_document: default
  # word_document: default
  # html_document: default
  #rmdformats::readthedown
  html_notebook:
    toc: true
    toc_float: true
    toc_collapsed: true
---



##. In this script I will remove Non T cells from PBMC 

# 1. load libraries
```{r setup, include=FALSE}

library(Seurat)
library(SeuratObject)
library(SeuratData)
library(patchwork)

library(dplyr)
library(tidyverse)
library(ggplot2)
library(RColorBrewer)
library(magrittr)
library(dbplyr)
library(rmarkdown)
library(knitr)
library(tinytex)
#Azimuth Annotation libraries
library(Azimuth)
#ProjecTils Annotation libraries
library(STACAS)
library(ProjecTILs)
#singleR Annotation libraries
library(SingleR)
library(celldex)
library(SingleCellExperiment)

library(clustree)

```


# 2. Load Seurat Object 
```{r load_seurat}

#Load Seurat Object merged from cell lines and a control(PBMC) after filtration
load("../0-R_Objects/All_Samples_Merged_with_10x_Azitmuth_Annotated.robj")


All_samples_Merged


```

##  Cell type Distribution to check clusters
```{r Distribution1, fig.height=10, fig.width=10}
# We can apply it later on R obj to get these tables to compare it to decide about resolution.

# Azimuth l1
janitor::tabyl(All_samples_Merged@meta.data, predicted.celltype.l1, cell_line)


# Azimuth l2
janitor::tabyl(All_samples_Merged@meta.data, predicted.celltype.l2, cell_line)

# Azimuth l3
janitor::tabyl(All_samples_Merged@meta.data, predicted.celltype.l3, cell_line)

```

##  Cell type Distribution barplot
```{r Distribution2, fig.height=6, fig.width=10}

library(ggplot2)
library(RColorBrewer)  

# Assuming you have 10 different cell lines, generating a color palette with 10 colors
cell_line_colors <- brewer.pal(10, "Set3")

# Assuming All_samples_Merged$cell_line is a factor or character vector containing cell line names
data <- as.data.frame(table(All_samples_Merged$cell_line))
colnames(data) <- c("cell_line", "nUMI")  # Change column name to nUMI

ncells <- ggplot(data, aes(x = cell_line, y = nUMI, fill = cell_line)) + 
  geom_col() +
  theme_classic() +
  geom_text(aes(label = nUMI), 
            position = position_dodge(width = 0.9), 
            vjust = -0.25) +
  scale_fill_manual(values = cell_line_colors) + 
  theme(axis.text.x = element_text(angle = 45, hjust = 1),
        plot.title = element_text(hjust = 0.5)) +  # Adjust the title position
  ggtitle("Filtered cells per sample") +
  xlab("Cell lines") +  # Adjust x-axis label
  ylab("Frequency")    # Adjust y-axis label

print(ncells)


```

# 3. filter cells just keep CD4 T cells
```{r Distribution3, fig.height=6, fig.width=10}

# Set identity to cell_line 
Idents(All_samples_Merged) <- "cell_line"

# Identify CD4 T cells in PBMC and PBMC_10x
cd4_cells_pbmc <- WhichCells(All_samples_Merged, expression = 
  grepl("^CD4", All_samples_Merged$predicted.celltype.l1) &
  grepl("^CD4", All_samples_Merged$predicted.celltype.l2) &
  grepl("^CD4", All_samples_Merged$predicted.celltype.l3) &
  (cell_line %in% c("PBMC", "PBMC_10x"))
)

# Select all cells from other cell lines
other_cells_seurat <- subset(All_samples_Merged, subset = cell_line %in% c("L1", "L2", "L3", "L4", "L5", "L6", "L7"))

# Get the cell names from the subset
other_cells <- colnames(other_cells_seurat)

# Combine the cell lists
cells_to_keep <- c(cd4_cells_pbmc, other_cells)

# Create the final filtered Seurat object
filtered_seurat <- subset(All_samples_Merged, cells = cells_to_keep)



library(ggplot2)
library(RColorBrewer)  

# Assuming you have 10 different cell lines, generating a color palette with 10 colors
cell_line_colors <- brewer.pal(10, "Set3")

# Assuming All_samples_Merged$cell_line is a factor or character vector containing cell line names
data <- as.data.frame(table(filtered_seurat$cell_line))
colnames(data) <- c("cell_line", "nUMI")  # Change column name to nUMI

ncells <- ggplot(data, aes(x = cell_line, y = nUMI, fill = cell_line)) + 
  geom_col() +
  theme_classic() +
  geom_text(aes(label = nUMI), 
            position = position_dodge(width = 0.9), 
            vjust = -0.25) +
  scale_fill_manual(values = cell_line_colors) + 
  theme(axis.text.x = element_text(angle = 45, hjust = 1),
        plot.title = element_text(hjust = 0.5)) +  # Adjust the title position
  ggtitle("Filtered cells per sample") +
  xlab("Cell lines") +  # Adjust x-axis label
  ylab("Frequency")    # Adjust y-axis label

print(ncells)


```




## Cell type Distribution to check clusters
```{r Distribution4, fig.height=6, fig.width=10}

# We can apply it later on R obj to get these tables to compare it to decide about resolution.


janitor::tabyl(filtered_seurat@meta.data, predicted.celltype.l1, cell_line)


janitor::tabyl(filtered_seurat@meta.data, predicted.celltype.l2, cell_line)


janitor::tabyl(filtered_seurat@meta.data, predicted.celltype.l3, cell_line)

```


# 4. filter B cells from L4
```{r Distribution5, fig.height=6, fig.width=10}

# Set identity to cell_line
Idents(filtered_seurat) <- "cell_line"


# Load necessary libraries
library(Seurat)
library(ggplot2)
library(RColorBrewer)

# Identify B cells in L4 to exclude
b_cells_l4 <- WhichCells(filtered_seurat, expression = 
  grepl("^B", filtered_seurat$predicted.celltype.l1) &
  grepl("^B", filtered_seurat$predicted.celltype.l2) &
  grepl("^B", filtered_seurat$predicted.celltype.l3) &
  cell_line == "L4"
)

# Identify cells to keep (excluding B cells in L4)
cells_to_keep <- setdiff(Cells(filtered_seurat), b_cells_l4)

# Subset the Seurat object with selected cells
filtered_seurat <- subset(filtered_seurat, cells = cells_to_keep)

# Plot the filtered data

# Define cell line colors
cell_line_colors <- brewer.pal(length(unique(filtered_seurat$cell_line)), "Set3")

# Generate data frame for plotting
data <- as.data.frame(table(filtered_seurat$cell_line))
colnames(data) <- c("cell_line", "nUMI")

# Create the bar plot
ncells <- ggplot(data, aes(x = cell_line, y = nUMI, fill = cell_line)) +
  geom_col() +
  theme_classic() +
  geom_text(aes(label = nUMI), position = position_dodge(width = 0.9), vjust = -0.25) +
  scale_fill_manual(values = cell_line_colors) +
  theme(axis.text.x = element_text(angle = 45, hjust = 1),
        plot.title = element_text(hjust = 0.5)) +
  ggtitle("Filtered Cells per Sample") +
  xlab("Cell Lines") +
  ylab("Frequency")

print(ncells)

```

## Cell type Distribution to check clusters
```{r Distribution6, fig.height=10, fig.width=10}
# We can apply it later on R obj to get these tables to compare it to decide about resolution.

# Azimuth l1
janitor::tabyl(filtered_seurat@meta.data, predicted.celltype.l1, cell_line)

# Azimuth l2
janitor::tabyl(filtered_seurat@meta.data, predicted.celltype.l2, cell_line)

# Azimuth l3
janitor::tabyl(filtered_seurat@meta.data, predicted.celltype.l3, cell_line)


```





# 5. Save the Seurat object as an Robj file
```{r saveROBJ, echo=FALSE}

save(filtered_seurat, file = "../0-Robj/SS_CD4_Tcells_PBMC10x_final_for_SCT_AzimuthAnnotation_and_Integration.robj")


```







