library(readxl)
## Warning: package 'readxl' was built under R version 4.4.2
bird_Ohio <- read_excel("C:/Users/LENOVO/Downloads/bird_Ohio.xlsx")
## New names:
## • `` -> `...1`
env_Ohio <- read_excel("env_Ohio.xlsx")
## New names:
## • `` -> `...1`
library(vegan)
## Warning: package 'vegan' was built under R version 4.4.2
## Loading required package: permute
## Warning: package 'permute' was built under R version 4.4.2
## Loading required package: lattice
## This is vegan 2.6-8
bird_Ohio
ncol(bird_Ohio)
## [1] 49
sp.rich<-specnumber(bird_Ohio, MARGIN=1)
as.data.frame(sp.rich)
sp.even<-specnumber(bird_Ohio,MARGIN=2)
as.data.frame(sp.even)
shannon<-diversity(bird_Ohio, index = "shannon")
shannon
## [1] 1.8917399 2.5122269 2.5759511 2.5306341 2.5813695 2.5851922 2.8543118
## [8] 2.6869090 2.6290101 2.4471082 2.4514742 2.6624436 2.4801533 2.4524231
## [15] 2.4446459 2.5985159 2.1580650 2.0290131 2.3439848 2.3677896 2.6230152
## [22] 2.1818266 2.3999167 2.3497389 2.1019228 2.1558323 2.1542864 2.2277483
## [29] 2.3289428 1.8809617 2.4493401 2.3266446 2.2270348 1.9964440 2.0417370
## [36] 2.4618897 2.1320689 2.2922720 1.5430953 2.0627115 2.6546098 2.3331170
## [43] 1.7535317 1.8944254 1.9905828 1.8202840 1.7588983 1.2049611 2.1437307
## [50] 2.0737966 1.6534827 2.1077805 1.9625262 1.7738141 2.0210011 1.8759969
## [57] 1.4522335 1.5969316 1.4228140 2.2233828 1.6941131 2.2527933 1.4705667
## [64] 1.4376671 1.6556983 2.0069460 1.7242396 1.6262868 1.9412633 1.2809809
## [71] 1.3010760 1.5318794 1.5531853 1.5204937 1.7648837 1.6943305 1.7000499
## [78] 1.3091997 1.5702975 1.3837769 1.3385135 1.4513903 0.9429183 1.4490505
## [85] 1.7895111 1.9656594 1.5788314 1.5022623 1.4904512 1.4962931 1.4059042
## [92] 1.6478061 1.2990656 1.0930459 1.5543977 1.7289643 1.6443609 1.5495614
## [99] 1.4031313 1.7226377 1.8883235 1.9303606 1.4901640 1.5787674 1.5705318
## [106] 1.4178638 1.2837894 1.5926701 1.6081552 1.4082954 1.2234179 1.2528753
## [113] 1.3100790 1.4507992 1.2323738 1.3797947 1.7293521 1.4854109 1.4658616
## [120] 0.9669648 1.1795715 1.3672704 1.5350481 1.3090028 1.2126036 1.3047201
## [127] 1.2040135 1.3639687 1.1349332 1.4067959 1.2394246 1.3198667 1.1560033
## [134] 1.2668167 1.3633187 1.7184293 1.4705114 1.4134997 1.1657677 1.4382794
## [141] 1.6241226 0.9479850 0.7890530 1.4545973 1.4366209 1.3270892 1.1408832
## [148] 0.9003295 0.9349969 1.1903317 1.5749987 1.5003813 1.0810697 1.5488876
## [155] 1.5759803 0.9573217 1.2080605 1.1433944 1.0993195 1.1174376 1.1019272
## [162] 0.9943400 1.0646499 1.6364990 1.2202461 1.3055935 1.2551904 1.3908337
## [169] 1.5319849 0.9827985 0.9091475 1.3284004 1.0280144 1.0155201 0.8256294
## [176] 1.1407526 1.1095773 0.9370976 1.1957853 0.9132408 0.9268789 0.9070407
## [183] 0.9184634 1.0505849 1.1130730 1.1475092 0.8861070 1.1679846 1.3269686
## [190] 1.4025824 0.7316835 1.0732068 1.0880649 0.8289934 0.8676827 1.1044350
## [197] 1.0755235 1.0144104 1.1081660 1.0762302 1.0511711 0.9282779 1.2321592
## [204] 0.7472004 0.8967415 0.9696155 0.4879819 1.2601278 1.2607309 1.0734488
simpson<-diversity(bird_Ohio, index = "simpson")
simpson
## [1] 0.7923875 0.9023669 0.9141051 0.8955078 0.9112426 0.9100346 0.9286265
## [8] 0.9171429 0.9131944 0.8927116 0.8897290 0.9137893 0.8793388 0.8758573
## [15] 0.8830959 0.8967347 0.8536155 0.8264046 0.8624852 0.8617998 0.8762865
## [22] 0.8433163 0.8526139 0.8573088 0.8130987 0.8250548 0.8136574 0.8264701
## [29] 0.8491358 0.7441406 0.8553590 0.8238062 0.8106576 0.7780612 0.7801904
## [36] 0.8531268 0.7831946 0.8068698 0.6477631 0.7808963 0.8616564 0.7890625
## [43] 0.6954194 0.7339693 0.7269136 0.6911844 0.6822566 0.5081633 0.7527571
## [50] 0.7694515 0.6567901 0.7160000 0.7361333 0.6479362 0.6747189 0.6863983
## [57] 0.5658574 0.6459054 0.5912465 0.7710506 0.6341785 0.7706531 0.5559896
## [64] 0.5789628 0.6272199 0.7150879 0.6403025 0.6024793 0.7088021 0.4988000
## [71] 0.4968140 0.5861804 0.5995339 0.5778835 0.6374853 0.6346974 0.6039282
## [78] 0.4959013 0.5915848 0.5347080 0.5234094 0.5429308 0.4238292 0.5318678
## [85] 0.6617778 0.6964237 0.5921019 0.5775047 0.5791837 0.5853587 0.5223585
## [92] 0.6010774 0.5181661 0.4416889 0.5807846 0.6220482 0.5677222 0.5565515
## [99] 0.5220452 0.5952296 0.6635526 0.6503184 0.5207111 0.6158734 0.5447686
## [106] 0.5379747 0.4695946 0.5760346 0.5821278 0.5175781 0.5051503 0.4630629
## [113] 0.4962000 0.5478637 0.4671492 0.5235294 0.6090305 0.5106574 0.5185606
## [120] 0.3701524 0.4405887 0.5040980 0.5602648 0.4785325 0.4391298 0.4572742
## [127] 0.4574581 0.4963772 0.4396221 0.5037577 0.4670187 0.4978497 0.4104902
## [134] 0.4898061 0.4876454 0.5769861 0.5051541 0.5225468 0.3938079 0.5078587
## [141] 0.5466634 0.3572150 0.3180077 0.4964437 0.4751863 0.4889245 0.4303415
## [148] 0.3341988 0.3763435 0.4262014 0.5111597 0.5117908 0.4075500 0.5420794
## [155] 0.5161432 0.3428280 0.4343764 0.4120049 0.4084227 0.3921670 0.4009981
## [162] 0.3344865 0.4009879 0.5320156 0.4317769 0.4590037 0.4250527 0.4708680
## [169] 0.5338560 0.3637872 0.3495779 0.4747466 0.3657520 0.3795146 0.2896172
## [176] 0.4219639 0.4203375 0.3414050 0.4325555 0.3431800 0.3372680 0.3009006
## [183] 0.3442333 0.3724143 0.3960015 0.3812779 0.3234457 0.4113292 0.4541780
## [190] 0.4803133 0.2576901 0.3719623 0.3935743 0.2681985 0.3192152 0.3765746
## [197] 0.3795178 0.3771101 0.3910828 0.3889234 0.3824131 0.3371236 0.4337904
## [204] 0.2852947 0.3188013 0.3529198 0.1674397 0.4239074 0.4304125 0.3842678
inv.simpson<-diversity(bird_Ohio, index = "invsimpson")
inv.simpson
## [1] 4.816667 10.242424 11.642140 9.570093 11.266667 11.115385 14.010811
## [8] 12.068966 11.520000 9.320675 9.068571 11.599483 8.287671 8.055249
## [15] 8.554023 9.683794 6.831325 5.760522 7.271945 7.235880 8.083192
## [22] 6.382284 6.784902 7.008138 5.350417 5.716075 5.366460 5.762696
## [29] 6.628478 3.908397 6.913669 5.675570 5.281437 4.505747 4.549391
## [36] 6.808596 4.612431 5.177854 2.838998 4.564050 7.228380 4.740741
## [43] 3.283203 3.758965 3.661844 3.238179 3.147193 2.033195 4.044605
## [50] 4.337483 2.913669 3.521127 3.789793 2.840394 3.074264 3.188759
## [57] 2.303391 2.824104 2.446462 4.367778 2.733574 4.360207 2.252199
## [64] 2.375087 2.682547 3.509854 2.780114 2.515593 3.434090 1.995211
## [71] 1.987337 2.416512 2.497090 2.369014 2.758509 2.737457 2.524795
## [78] 1.983739 2.448489 2.149188 2.098237 2.187852 1.735597 2.136149
## [85] 2.956636 3.294065 2.451592 2.366890 2.376334 2.411723 2.093621
## [92] 2.506752 2.075404 1.791116 2.385409 2.645840 2.313327 2.255053
## [99] 2.092248 2.470536 2.972233 2.859744 2.086424 2.603309 2.196685
## [106] 2.164384 1.885350 2.358683 2.393076 2.072874 2.020815 1.862416
## [113] 1.984915 2.211722 1.876698 2.098765 2.557744 2.043558 2.077105
## [120] 1.587686 1.787593 2.016527 2.274096 1.917665 1.782944 1.842551
## [127] 1.843175 1.985613 1.784510 2.015144 1.876238 1.991436 1.696325
## [134] 1.960039 1.951773 2.363989 2.020831 2.094446 1.649642 2.031937
## [141] 2.205866 1.555730 1.466292 1.985875 1.905438 1.956658 1.755438
## [148] 1.501950 1.603447 1.742772 2.045658 2.048302 1.687906 2.183785
## [155] 2.066727 1.521672 1.767960 1.700695 1.690396 1.645189 1.669444
## [162] 1.502599 1.669415 2.136824 1.759872 1.848441 1.739290 1.889888
## [169] 2.145260 1.571801 1.537463 1.903843 1.576670 1.611642 1.407692
## [176] 1.729996 1.725142 1.518384 1.762287 1.522487 1.508906 1.430412
## [183] 1.524933 1.593408 1.655633 1.616235 1.478078 1.698742 1.832099
## [190] 1.924236 1.347146 1.592261 1.649007 1.366491 1.468893 1.604041
## [197] 1.611650 1.605420 1.642259 1.636456 1.619205 1.508577 1.766130
## [204] 1.399178 1.468000 1.545403 1.201114 1.735832 1.755657 1.624083
fish.alp<-fisher.alpha(bird_Ohio)
fish.alp
## [1] 5.642066 7.896600 6.488217 7.565648 7.896600 7.275326 11.680602
## [8] 8.578491 7.703286 7.612305 7.916266 8.839863 9.316717 9.454690
## [15] 7.814550 9.354043 4.970140 4.970140 6.781181 7.415136 11.156386
## [22] 5.110244 9.198544 7.705935 5.389613 5.679263 6.377738 6.055108
## [29] 6.765902 4.927590 7.224522 8.920683 7.027652 6.430958 5.381526
## [36] 8.310228 6.891754 8.984035 3.690822 5.538659 11.970603 9.586698
## [43] 4.350582 5.112918 6.765902 5.254828 4.718079 3.192559 7.976087
## [50] 5.576450 3.718729 9.354332 5.646225 6.096825 10.837223 5.798205
## [57] 4.212510 4.392532 3.217794 7.064403 4.829737 8.991549 4.986195
## [64] 3.546422 4.711590 6.648249 5.988234 6.114229 6.065899 3.560586
## [71] 3.546422 4.192993 5.427384 5.030672 5.726751 5.687885 6.259378
## [78] 4.238987 4.858106 4.121275 3.717707 5.349775 1.659647 4.890459
## [85] 4.931276 7.342929 5.082987 4.281400 4.257961 4.223961 4.342856
## [92] 5.369842 3.173985 2.544462 4.545684 6.048954 7.602821 5.778996
## [99] 5.414712 7.383522 6.606168 7.981444 7.105981 3.601971 6.964669
## [106] 4.071694 4.557373 5.109555 5.469168 4.810390 3.335706 4.489371
## [113] 4.415941 4.311580 4.062475 3.967505 6.848485 7.622560 6.259936
## [120] 3.401558 4.396019 5.031600 5.661968 4.672620 5.506903 6.274631
## [127] 3.935634 4.959159 3.588003 5.297514 3.882807 3.497519 5.042383
## [134] 4.164308 5.639364 8.224130 6.763796 4.777415 5.769443 5.149475
## [141] 7.797693 3.904973 2.305868 5.871991 7.955229 4.394232 3.450861
## [148] 3.204387 2.846412 4.802582 8.995673 6.871785 3.756033 6.745033
## [155] 8.446768 3.800210 4.713438 4.046218 4.379899 5.104476 4.033430
## [162] 5.926942 4.014640 8.614307 5.345689 5.647828 6.075704 6.342263
## [169] 6.915739 3.675202 2.750911 4.849896 4.653569 3.632950 3.708700
## [176] 4.551192 3.574193 3.328464 4.513385 3.010270 3.314740 5.730437
## [183] 3.296916 4.218990 4.513385 5.923958 3.292541 4.471297 6.113701
## [190] 6.406272 3.014282 5.165024 4.453883 5.647828 2.957055 6.182347
## [197] 4.772041 3.494964 4.741473 4.409155 4.409155 3.815290 4.655622
## [204] 2.103496 3.815290 3.778652 2.415700 6.323830 5.950260 4.993876
Div.Ind<-cbind.data.frame(shannon, simpson, inv.simpson,fish.alp)
Div.Ind
summary(Div.Ind)
## shannon simpson inv.simpson fish.alp
## Min. :0.488 Min. :0.1674 Min. : 1.201 Min. : 1.660
## 1st Qu.:1.171 1st Qu.:0.4272 1st Qu.: 1.746 1st Qu.: 4.214
## Median :1.453 Median :0.5277 Median : 2.117 Median : 5.112
## Mean :1.559 Mean :0.5678 Mean : 3.192 Mean : 5.521
## 3rd Qu.:1.891 3rd Qu.:0.6944 3rd Qu.: 3.272 3rd Qu.: 6.638
## Max. :2.854 Max. :0.9286 Max. :14.011 Max. :11.971
env_Ohio
Ohio.env.Div<-cbind.data.frame(env_Ohio, Div.Ind)
Ohio.env.Div
library("rstatix")
## Warning: package 'rstatix' was built under R version 4.4.2
##
## Attaching package: 'rstatix'
## The following object is masked from 'package:stats':
##
## filter
Ohio.env.Div %>%
group_by(Habitat) %>%
get_summary_stats(shannon, type = "mean_sd")
Ohio.env.Div %>%
group_by(Habitat) %>%
get_summary_stats(simpson, type = "mean_sd")
Ohio.env.Div %>%
group_by(Habitat) %>%
get_summary_stats(fish.alp, type = "mean_sd")
Ohio.env.Div %>%
group_by(Habitat) %>%
get_summary_stats(inv.simpson, type = "mean_sd")
library("ggplot2")
## Warning: package 'ggplot2' was built under R version 4.4.2
Shanon.habitat<-ggplot(Ohio.env.Div, aes(x = Habitat, y = shannon, fill = Habitat)) +
geom_boxplot() +
stat_summary(fun = mean, geom = "point", shape =21, size = 3, colour = "black", fill="yellow")
Simp.habitat<-ggplot(Ohio.env.Div, aes(x = Habitat, y = simpson, fill = Habitat)) +
geom_boxplot() +
stat_summary(fun = mean, geom = "point", shape =21, size = 3, colour = "black", fill="yellow")
InvSimp.habitat<-ggplot(Ohio.env.Div, aes(x = Habitat, y = inv.simpson, fill = Habitat)) +
geom_boxplot() +
stat_summary(fun = mean, geom = "point", shape =21, size = 3, colour = "black", fill="yellow")
Fish.habitat<-ggplot(Ohio.env.Div, aes(x = Habitat, y = fish.alp, fill = Habitat)) +
geom_boxplot() +
stat_summary(fun = mean, geom = "point", shape =21, size = 3, colour = "black", fill="yellow")
library("gridExtra")
## Warning: package 'gridExtra' was built under R version 4.4.2
grid.arrange(Shanon.habitat, Simp.habitat, InvSimp.habitat, Fish.habitat,
nrow=2, ncol=2)

grid.arrange(Shanon.habitat, Simp.habitat, InvSimp.habitat, Fish.habitat, nrow=2, ncol=2)

shannon_aov <- aov(Ohio.env.Div$shannon ~ Ohio.env.Div$Habitat)
summary(shannon_aov)
## Df Sum Sq Mean Sq F value Pr(>F)
## Ohio.env.Div$Habitat 2 1.66 0.8308 3.335 0.0375 *
## Residuals 207 51.57 0.2491
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
simpson_aov <- aov(Ohio.env.Div$simpson ~ Ohio.env.Div$Habitat)
summary(simpson_aov)
## Df Sum Sq Mean Sq F value Pr(>F)
## Ohio.env.Div$Habitat 2 0.189 0.09426 3.015 0.0512 .
## Residuals 207 6.471 0.03126
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
inv.simpson_aov <- aov(Ohio.env.Div$inv.simpson ~ Ohio.env.Div$Habitat)
summary(inv.simpson_aov)
## Df Sum Sq Mean Sq F value Pr(>F)
## Ohio.env.Div$Habitat 2 18.2 9.109 1.481 0.23
## Residuals 207 1273.6 6.153
fish.alp_aov <- aov(Ohio.env.Div$fish.alp ~ Ohio.env.Div$Habitat)
summary(fish.alp_aov)
## Df Sum Sq Mean Sq F value Pr(>F)
## Ohio.env.Div$Habitat 2 15.4 7.687 2.206 0.113
## Residuals 207 721.4 3.485
distance_matrix<-vegdist(Ohio.env.Div[,10:10], method="bray", binary=FALSE)
adonis2(distance_matrix ~ Habitat, data=Ohio.env.Div)
shannon_Tukey<-TukeyHSD(shannon_aov, conf.level=.95, ordered = TRUE)
shannon_Tukey
## Tukey multiple comparisons of means
## 95% family-wise confidence level
## factor levels have been ordered
##
## Fit: aov(formula = Ohio.env.Div$shannon ~ Ohio.env.Div$Habitat)
##
## $`Ohio.env.Div$Habitat`
## diff lwr upr p adj
## wet-mesic-dry-oak 0.14147731 -0.058161073 0.3411157 0.2180524
## dry-mesic-dry-oak 0.20188576 0.007587024 0.3961845 0.0396316
## dry-mesic-wet-mesic 0.06040845 -0.159546381 0.2803633 0.7935133
simpson_Tukey<-TukeyHSD(simpson_aov, conf.level=.95, ordered = TRUE)
simpson_Tukey
## Tukey multiple comparisons of means
## 95% family-wise confidence level
## factor levels have been ordered
##
## Fit: aov(formula = Ohio.env.Div$simpson ~ Ohio.env.Div$Habitat)
##
## $`Ohio.env.Div$Habitat`
## diff lwr upr p adj
## wet-mesic-dry-oak 0.04373077 -0.0269916799 0.1144532 0.3125264
## dry-mesic-dry-oak 0.06936909 0.0005382234 0.1382000 0.0477573
## dry-mesic-wet-mesic 0.02563832 -0.0522812927 0.1035579 0.7177126
inv.simpson_Tukey<-TukeyHSD(inv.simpson_aov, conf.level=.95, ordered = TRUE)
inv.simpson_Tukey
## Tukey multiple comparisons of means
## 95% family-wise confidence level
## factor levels have been ordered
##
## Fit: aov(formula = Ohio.env.Div$inv.simpson ~ Ohio.env.Div$Habitat)
##
## $`Ohio.env.Div$Habitat`
## diff lwr upr p adj
## wet-mesic-dry-oak 0.06042921 -0.9317123 1.052571 0.9886676
## dry-mesic-dry-oak 0.67188001 -0.2937251 1.637485 0.2301703
## dry-mesic-wet-mesic 0.61145080 -0.4816572 1.704559 0.3853924
fish.alp_Tukey<-TukeyHSD(fish.alp_aov, conf.level=.95, ordered = TRUE)
fish.alp_Tukey
## Tukey multiple comparisons of means
## 95% family-wise confidence level
## factor levels have been ordered
##
## Fit: aov(formula = Ohio.env.Div$fish.alp ~ Ohio.env.Div$Habitat)
##
## $`Ohio.env.Div$Habitat`
## diff lwr upr p adj
## wet-mesic-dry-oak 0.538910024 -0.2077869 1.2856069 0.2061985
## dry-mesic-dry-oak 0.547843742 -0.1788816 1.2745691 0.1789658
## dry-mesic-wet-mesic 0.008933717 -0.8137517 0.8316192 0.9996378
shannon_Tukey_plot <- as.data.frame(shannon_Tukey$`Ohio.env.Div$Habitat`)
shannon_Tukey_plot$comparison <- rownames(shannon_Tukey_plot)
shannon_Tukey_plot
simpson_Tukey_plot <- as.data.frame(simpson_Tukey$`Ohio.env.Div$Habitat`)
simpson_Tukey_plot$comparison <- rownames(simpson_Tukey_plot)
simpson_Tukey_plot
inv.simpson_Tukey_plot <- as.data.frame(inv.simpson_Tukey$`Ohio.env.Div$Habitat`)
inv.simpson_Tukey_plot$comparison <- rownames(inv.simpson_Tukey_plot)
inv.simpson_Tukey_plot
fish.alp_Tukey_plot <- as.data.frame(fish.alp_Tukey$`Ohio.env.Div$Habitat`)
fish.alp_Tukey_plot$comparison <- rownames(fish.alp_Tukey_plot)
fish.alp_Tukey_plot
shannon_Tukey_plot_result<-ggplot(shannon_Tukey_plot, aes(x = comparison, y = diff)) +
geom_point() +
geom_errorbar(aes(ymin = lwr, ymax = upr), width = 0.2) +
labs(
title = "Tukey HSD Test Results for Shannon Diversity",
x = "Habitat Comparison",
y = "Pairwise Difference in Mean"
)
shannon_Tukey_plot_result

shannon_Tukey_plot_result_meandiff<-shannon_Tukey_plot_result +
theme(axis.text.x = element_text(angle = 45, hjust = 1)) +
geom_hline(yintercept=0, linetype="dashed", color = "red")
shannon_Tukey_plot_result_meandiff

simpson_Tukey_plot_result<-ggplot(simpson_Tukey_plot, aes(x = comparison, y = diff)) +
geom_point() +
geom_errorbar(aes(ymin = lwr, ymax = upr), width = 0.2) +
labs(
title = "Tukey HSD Test Results for Simpson Diversity",
x = "Habitat Comparison",
y = "Pairwise Difference in Mean"
)
simpson_Tukey_plot_result

simpson_Tukey_plot_result_meandiff<-simpson_Tukey_plot_result +
theme(axis.text.x = element_text(angle = 45, hjust = 1)) +
geom_hline(yintercept=0, linetype="dashed", color = "red")
simpson_Tukey_plot_result_meandiff

inv.simpson_Tukey_plot_result<-ggplot(inv.simpson_Tukey_plot, aes(x = comparison, y = diff)) +
geom_point() +
geom_errorbar(aes(ymin = lwr, ymax = upr), width = 0.2) +
labs(
title = "Tukey HSD Test Results for inv.Simpson Diversity",
x = "Habitat Comparison",
y = "Pairwise Difference in Mean"
)
inv.simpson_Tukey_plot_result

inv.simpson_Tukey_plot_result_meandiff<-inv.simpson_Tukey_plot_result +
theme(axis.text.x = element_text(angle = 45, hjust = 1)) +
geom_hline(yintercept=0, linetype="dashed", color = "red")
inv.simpson_Tukey_plot_result_meandiff

fish.alp_Tukey_plot_result<-ggplot(fish.alp_Tukey_plot, aes(x = comparison, y = diff)) +
geom_point() +
geom_errorbar(aes(ymin = lwr, ymax = upr), width = 0.2) +
labs(
title = "Tukey HSD Test Results for fish alpha Diversity",
x = "Habitat Comparison",
y = "Pairwise Difference in Mean"
)
fish.alp_Tukey_plot_result

fish.alp_Tukey_plot_result_meandiff<-fish.alp_Tukey_plot_result +
theme(axis.text.x = element_text(angle = 45, hjust = 1)) +
geom_hline(yintercept=0, linetype="dashed", color = "red")
fish.alp_Tukey_plot_result_meandiff

grid.arrange(shannon_Tukey_plot_result_meandiff,Shanon.habitat,nrow=1, ncol=2)

grid.arrange(simpson_Tukey_plot_result_meandiff,Simp.habitat,nrow=1, ncol=2)

grid.arrange(inv.simpson_Tukey_plot_result_meandiff,InvSimp.habitat,nrow=1, ncol=2)

grid.arrange(fish.alp_Tukey_plot_result_meandiff,Fish.habitat,nrow=1, ncol=2)
