Section

Other anlayses

Weight trajectory

Definitely different

Sex Genotype p
Males Tp53 0.0000118
Females Tp53 0.0000000
Males WT 0.0000003
Females WT 0.0000000

Lean Trajectory

Belive it or not, a little different

Sex Genotype p
Males Tp53 0.0138988
Females Tp53 0.0004708
Males WT 0.0032615
Females WT 0.0000000

Fat Trajectory

Very different

Sex Genotype p
Males Tp53 0
Females Tp53 0
Males WT 0
Females WT 0

Prevalence

We want to know if prevalence of cancer differs between HFD and NC. Not enough evidence of a difference and not enough evidence to establish equivalence.

Female: diet differenc by Genotypes
contrast genotype estimate SE df z.ratio p.value
HFD - NC Tp53 0.0640029 0.5883106 Inf 0.1087910 0.9133683
HFD - NC WT -1.6094379 2.1447611 Inf -0.7504043 0.4530112
Female: Genotype difference by diet
contrast diet estimate SE df z.ratio p.value
Tp53 - WT HFD 1.2602536 1.066926 Inf 1.1812007 0.2375230
Tp53 - WT NC -0.4131872 1.951353 Inf -0.2117439 0.8323068 
##                        2.5 %    97.5 %
## (Intercept)        0.5312869 1.9892203
## dietNC            -1.2170704 1.0890647
## genotypeWT        -3.3513900 0.8308827
## dietNC:genotypeWT -2.6854897 6.0323713
Male: diet differenc by Genotypes
contrast genotype estimate SE df z.ratio p.value
HFD - NC Tp53 0.4161604 0.7592179 Inf 0.5481436 0.5835933
HFD - NC WT 0.2513144 1.2445011 Inf 0.2019399 0.8399637
Male: Genotype difference by diet
contrast diet estimate SE df z.ratio p.value
Tp53 - WT HFD 1.0651364 1.000254 Inf 1.064866 0.2869366
Tp53 - WT NC 0.9002904 1.060513 Inf 0.848920 0.3959258 
##                        2.5 %    97.5 %
## (Intercept)        0.6258908 2.6799552
## dietNC            -1.9042001 1.0718793
## genotypeWT        -3.0255980 0.8953252
## dietNC:genotypeWT -2.6923989 3.0220909

Burden

Not enough evidence of a difference and not enough evidence to establish equivalence.

Female: diet differences
contrast genotype estimate SE df z.ratio p.value
HFD - NC Tp53 0.1813558 0.2037621 Inf 0.8900371 0.3734460
HFD - NC WT 0.4054651 0.8164933 Inf 0.4965933 0.6194759
Female: genotype differences
contrast diet estimate SE df z.ratio p.value
Tp53 - WT HFD 0.0909718 0.4256217 Inf 0.2137386 0.8307509
Tp53 - WT NC 0.3150810 0.7259659 Inf 0.4340163 0.6642766 
##                        2.5 %    97.5 %
## (Intercept)        0.2508246 0.7234578
## genotypeWT        -1.0392268 0.6591542
## dietNC            -0.5901667 0.2113200
## genotypeWT:dietNC -2.1782800 1.3000262
Male: diet differences
contrast genotype estimate SE df z.ratio p.value
HFD - NC Tp53 0.1094173 0.1994346 Inf 0.5486376 0.5832542
HFD - NC WT -0.0645385 0.4859111 Inf -0.1328196 0.8943360
Male: genotype differences
contrast diet estimate SE df z.ratio p.value
Tp53 - WT HFD 0.4730853 0.3556600 Inf 1.3301615 0.1834651
Tp53 - WT NC 0.2991295 0.3865096 Inf 0.7739251 0.4389751 
##                        2.5 %    97.5 %
## (Intercept)        0.6251802 1.1121852
## genotypeWT        -1.2406734 0.1703689
## dietNC            -0.5073734 0.2769465
## genotypeWT:dietNC -0.8772612 1.2109772

QC and data processing

This section pertains entirely to data processing including removal of metabolites with too much missing data. Data are then normalized and missing measures are imputed.

No variance

These metabolites were removed due to 0 variance.

Removed for zero variance
Removed Metabolites
pn_Unk_286.11815_7.052
pn_Unk_316.04875_4.204
pn_Unk_189.9565_2.268
pn_Unk_191.86654_4.249
pn_Unk_438.29808_9.433
pn_EDDA_176.0797_6.226
pn_Unk_452.27733_8.888
pn_X4.Hydroxy.3.sulfooxy.benzoic.acid_233.98269_2.146
pn_X2.hydroxycaproicacid_132.07863_5.917
pn_X3alpha_7alpha_12beta.Trihydroxy.5beta.cholanateb_408.28764_8.921
pn_X3alpha_7alpha_12beta.Trihydroxy.5beta.cholanatec_408.28766_8.673
pn_DL.Erythrono.1_4.lactone.Erythrono.1_4.lactonec_118.02659_2.678
tp_L.Phenylalanine
tp_Diaveridine_260.12362_4.404
tp_Bufa.20.22.dienolide_354.2559_7.806
tp_Unk_479.2707_7.991
tp_Rimexolone_370.25083_8.059
tp_X2.methylbutyrylcarnitinee_245.16275_4.876
tp_X.nabiloned_372.26645_7.93
tp_threonylphenylalaninec_266.1267_3.563
tp_Unk_425.31418_8.629
tp_Unk_390.27702_8.63
tp_Unk_406.27202_8.099
tp_Unk_119.07352_3.973
tp_Unk_870.28063_1.122
tp_Unk_173.96155_1.187
tp_Unk_288.91817_1.087
tp_octinoxate_290.18825_6.737
tp_X2062850_358.2509_7.412
tp_Imidazoleaceticacid_126.04294_3.961
tp_Unk_273.61815_9.708
tp_Unk_422.89366_1.138
tp_Unk_954.19328_3.539
tp_X2_2.Iminodipropanoate_161.06882_1.352
tp_Unk_384.28523_11.795
tp_Unk_782.72789_1.118
tp_Unk_317.93024_1.068
tp_Unk_304.14995_4.614
tp_Unk_371.00841_1.155
tp_Unk_661.8109_1.064

Metabolites identifed per mouse

Missingness per subject

In the heatmaps below, white indicates a missing value, black indicates a value > limit-of-detection was detected. It’s difficult to see any obvious patterns here.

Is missingness correlated with a specific group

Individual groups

This looks to answer a similar question to the previous section. If the presence/absence of a metabolite was specific to a group or timepoint, we would see it in this section. Below are two heatmaps with the number of individuals with missing values for the metabolites listed on the right. The HFD has quite a few missing metabolites at 16 and 40 weeks. We unfotunately don’t have enough power to determine significance, here but the current pattern is striking. HFD 8w is very similar to NC 8w,16w, and 40w; so the metabolic changes haven’t fully taken effect yet. However HFD vs NC are the most distinct groups. The other groups cluster much more strongly on timepoint than genotype or Tj group.

Are metabolites with missing values often lower intensities?

Below we plot the cumulative density of metabolite measures by those that had missing data and those that did not have any. We see that metabolites with missing data tend to have lower values, thus our imputation is not missing at random and our imputation method will need to account for this left truncation/censoring.

Normalize

We perform variance stabilization and log2 transform data to better normalize measures for analysis. Data are plotted as: before and after VSN.

Impute

We will impute the missing values from a truncated distribution with parameters estimated using quantile regression. Plots are in order: cumulative intensity distributions prior to imputation, after imputation, and the amount of missingness per patient (now none)

Batch Effects

No real batch effects here

Data Exploration

PCA plot of the groups

HFD splits out convincingly in PC3 and PC4 starting at 16W

PLS-DA of the groups

All

Heatmap

HFD again in it’s own cluster. Difficult to immediately discern any other patterns, but we may want to dig into this more.

Additional Parameters

It looks like most of TjA is female in Trim28(+/D9) and TjB is male. That will make that comparison very complicated

Tp53(R270H/+)HFD
(N=30)		 Tp53(R270H/+)NC
(N=33)		 Tp53(R270H/+) Trim28(+/D9)TjB
(N=21)		 Tp53(R270H/+) Trim28(+/D9)TjA
(N=12)		 Trim28(+/D9)TjB
(N=18)		 Trim28(+/D9)TjA
(N=18)		 Overall
(N=132)
Batch
Batch 1 9 (30.0%) 9 (27.3%) 6 (28.6%) 6 (50.0%) 9 (50.0%) 3 (16.7%) 42 (31.8%)
Batch 2 9 (30.0%) 12 (36.4%) 9 (42.9%) 0 (0%) 6 (33.3%) 6 (33.3%) 42 (31.8%)
Batch 3 12 (40.0%) 12 (36.4%) 6 (28.6%) 6 (50.0%) 3 (16.7%) 9 (50.0%) 48 (36.4%)
Sex
Female 15 (50.0%) 18 (54.5%) 3 (14.3%) 6 (50.0%) 6 (33.3%) 9 (50.0%) 57 (43.2%)
Male 15 (50.0%) 15 (45.5%) 18 (85.7%) 6 (50.0%) 12 (66.7%) 9 (50.0%) 75 (56.8%)
Fat_mass
Mean (SD) 7.70 (7.14) 4.69 (4.73) 8.35 (5.69) 5.12 (5.42) 8.84 (7.42) 4.37 (4.36) 6.52 (6.09)
Median [Min, Max] 5.50 [1.06, 22.7] 2.17 [0.600, 21.3] 8.36 [1.61, 26.1] 2.43 [0.780, 16.3] 8.45 [1.76, 29.5] 2.63 [1.39, 17.4] 3.74 [0.600, 29.5]
Lean_mass
Mean (SD) 24.6 (5.89) 23.8 (4.86) 30.7 (7.03) 26.0 (6.60) 26.5 (5.85) 25.4 (6.28) 25.9 (6.28)
Median [Min, Max] 24.5 [16.2, 38.6] 23.5 [16.4, 33.5] 28.8 [17.6, 44.9] 26.0 [16.7, 39.1] 26.0 [17.8, 38.0] 25.2 [16.7, 42.0] 25.4 [16.2, 44.9]
Litter
10 3 (10.0%) 0 (0%) 0 (0%) 6 (50.0%) 0 (0%) 9 (50.0%) 18 (13.6%)
15 3 (10.0%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 3 (2.3%)
17 6 (20.0%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 6 (4.5%)
18 3 (10.0%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 3 (2.3%)
23 3 (10.0%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 3 (2.3%)
24 3 (10.0%) 3 (9.1%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 6 (4.5%)
25 3 (10.0%) 3 (9.1%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 6 (4.5%)
27 3 (10.0%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 3 (2.3%)
29 3 (10.0%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 3 (2.3%)
13 0 (0%) 3 (9.1%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 3 (2.3%)
14 0 (0%) 3 (9.1%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 3 (2.3%)
19 0 (0%) 3 (9.1%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 3 (2.3%)
20 0 (0%) 3 (9.1%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 3 (2.3%)
26 0 (0%) 9 (27.3%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 9 (6.8%)
28 0 (0%) 3 (9.1%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 3 (2.3%)
5 0 (0%) 3 (9.1%) 6 (28.6%) 0 (0%) 6 (33.3%) 0 (0%) 15 (11.4%)
1 0 (0%) 0 (0%) 3 (14.3%) 0 (0%) 3 (16.7%) 0 (0%) 6 (4.5%)
12 0 (0%) 0 (0%) 3 (14.3%) 0 (0%) 0 (0%) 0 (0%) 3 (2.3%)
3 0 (0%) 0 (0%) 3 (14.3%) 0 (0%) 0 (0%) 0 (0%) 3 (2.3%)
7 0 (0%) 0 (0%) 6 (28.6%) 0 (0%) 3 (16.7%) 3 (16.7%) 12 (9.1%)
11 0 (0%) 0 (0%) 0 (0%) 3 (25.0%) 0 (0%) 0 (0%) 3 (2.3%)
6 0 (0%) 0 (0%) 0 (0%) 3 (25.0%) 0 (0%) 0 (0%) 3 (2.3%)
2 0 (0%) 0 (0%) 0 (0%) 0 (0%) 6 (33.3%) 0 (0%) 6 (4.5%)
9 0 (0%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 6 (33.3%) 6 (4.5%)
Cage
108162 3 (10.0%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 3 (2.3%)
112788 3 (10.0%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 3 (2.3%)
113550 3 (10.0%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 3 (2.3%)
114392 3 (10.0%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 3 (2.3%)
114393 3 (10.0%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 3 (2.3%)
118565 3 (10.0%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 3 (2.3%)
119018 3 (10.0%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 3 (2.3%)
120708 3 (10.0%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 3 (2.3%)
122783 3 (10.0%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 3 (2.3%)
122959 3 (10.0%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 3 (2.3%)
111106 0 (0%) 3 (9.1%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 3 (2.3%)
111988 0 (0%) 3 (9.1%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 3 (2.3%)
114385 0 (0%) 3 (9.1%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 3 (2.3%)
117235 0 (0%) 3 (9.1%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 3 (2.3%)
117237 0 (0%) 3 (9.1%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 3 (2.3%)
118564 0 (0%) 3 (9.1%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 3 (2.3%)
121534 0 (0%) 3 (9.1%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 3 (2.3%)
122094 0 (0%) 3 (9.1%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 3 (2.3%)
122167 0 (0%) 6 (18.2%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 6 (4.5%)
123459 0 (0%) 3 (9.1%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 3 (2.3%)
110138 0 (0%) 0 (0%) 3 (14.3%) 0 (0%) 0 (0%) 0 (0%) 3 (2.3%)
112283 0 (0%) 0 (0%) 3 (14.3%) 0 (0%) 0 (0%) 0 (0%) 3 (2.3%)
113298 0 (0%) 0 (0%) 3 (14.3%) 0 (0%) 3 (16.7%) 0 (0%) 6 (4.5%)
113299 0 (0%) 0 (0%) 3 (14.3%) 0 (0%) 3 (16.7%) 0 (0%) 6 (4.5%)
118566 0 (0%) 0 (0%) 3 (14.3%) 0 (0%) 0 (0%) 0 (0%) 3 (2.3%)
118567 0 (0%) 0 (0%) 3 (14.3%) 0 (0%) 3 (16.7%) 0 (0%) 6 (4.5%)
124455 0 (0%) 0 (0%) 3 (14.3%) 0 (0%) 0 (0%) 0 (0%) 3 (2.3%)
115761 0 (0%) 0 (0%) 0 (0%) 3 (25.0%) 0 (0%) 0 (0%) 3 (2.3%)
122894 0 (0%) 0 (0%) 0 (0%) 6 (50.0%) 0 (0%) 3 (16.7%) 9 (6.8%)
123030 0 (0%) 0 (0%) 0 (0%) 3 (25.0%) 0 (0%) 0 (0%) 3 (2.3%)
109002 0 (0%) 0 (0%) 0 (0%) 0 (0%) 3 (16.7%) 0 (0%) 3 (2.3%)
109733 0 (0%) 0 (0%) 0 (0%) 0 (0%) 6 (33.3%) 0 (0%) 6 (4.5%)
119022 0 (0%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 3 (16.7%) 3 (2.3%)
122023 0 (0%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 3 (16.7%) 3 (2.3%)
122954 0 (0%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 3 (16.7%) 3 (2.3%)
123709 0 (0%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 6 (33.3%) 6 (4.5%)

Analysis

Volcano plot and differential expression

HFD vs NC

Metab variable value.x value.y p Significant lfc Label Unknown
2 pn_Allantoin PhenotypeHFD - PhenotypeNC 0.000 -1.101 7.078 Significant -1.101 pn_Allantoin Known
4 pn_Bisphenol.S_250.02932_1.299 PhenotypeHFD - PhenotypeNC 0.011 0.436 1.951 Significant 0.436 pn_Bisphenol.S_250.02932_1.299 Known
7 pn_Creatine PhenotypeHFD - PhenotypeNC 0.004 -0.357 2.359 Significant -0.357 pn_Creatine Known
12 pn_D..Maltoseb_388.12163_1.448 PhenotypeHFD - PhenotypeNC 0.000 3.495 7.078 Significant 3.495 pn_D..Maltoseb_388.12163_1.448 Known
21 pn_DL.Alanine PhenotypeHFD - PhenotypeNC 0.011 -0.290 1.948 Significant -0.290 pn_DL.Alanine Known
24 pn_DL.p.Hydroxyphenyllactic.acid PhenotypeHFD - PhenotypeNC 0.000 0.631 4.417 Significant 0.631 pn_DL.p.Hydroxyphenyllactic.acid Known
31 pn_Gluconic.acid_196.05828_1.605 PhenotypeHFD - PhenotypeNC 0.011 -0.737 1.976 Significant -0.737 pn_Gluconic.acid_196.05828_1.605 Known
32 pn_Gluconic.acidb PhenotypeHFD - PhenotypeNC 0.037 -0.706 1.431 Significant -0.706 pn_Gluconic.acidb Known
38 pn_L.Citrulline PhenotypeHFD - PhenotypeNC 0.010 -0.221 1.991 Significant -0.221 pn_L.Citrulline Known
41 pn_L.Hydroxyproline PhenotypeHFD - PhenotypeNC 0.000 -1.196 4.417 Significant -1.196 pn_L.Hydroxyproline Known
45 pn_L.Tyrosine PhenotypeHFD - PhenotypeNC 0.001 0.593 2.995 Significant 0.593 pn_L.Tyrosine Known
46 pn_Mannitol.or.isomer. PhenotypeHFD - PhenotypeNC 0.003 -1.520 2.556 Significant -1.520 pn_Mannitol.or.isomer. Known
50 pn_O.Phosphorylethanolamine PhenotypeHFD - PhenotypeNC 0.010 0.515 1.991 Significant 0.515 pn_O.Phosphorylethanolamine Known
51 pn_Ornithine PhenotypeHFD - PhenotypeNC 0.013 -0.224 1.886 Significant -0.224 pn_Ornithine Known
61 pn_Sedoheptulose.tent. PhenotypeHFD - PhenotypeNC 0.000 1.504 9.217 Significant 1.504 pn_Sedoheptulose.tent. Known
148 pn_Uridine PhenotypeHFD - PhenotypeNC 0.039 -0.408 1.407 Significant -0.408 pn_Uridine Known
157 pn_X1.O.Arsonopentofuranose_273.96618_0.825 PhenotypeHFD - PhenotypeNC 0.050 -0.263 1.303 Significant -0.263 pn_X1.O.Arsonopentofuranose_273.96618_0.825 Known
160 pn_X2.C.Methyl.D.erythritol4.phosphate_216.04006_1.366 PhenotypeHFD - PhenotypeNC 0.049 -0.217 1.313 Significant -0.217 pn_X2.C.Methyl.D.erythritol4.phosphate_216.04006_1.366 Known
172 tp_Acetyl.L.carnitine PhenotypeHFD - PhenotypeNC 0.011 -0.489 1.948 Significant -0.489 tp_Acetyl.L.carnitine Known
173 tp_Acetylcholine.tent. PhenotypeHFD - PhenotypeNC 0.000 -1.007 4.712 Significant -1.007 tp_Acetylcholine.tent. Known
175 tp_Betaine PhenotypeHFD - PhenotypeNC 0.000 -0.670 4.628 Significant -0.670 tp_Betaine Known
179 tp_Creatinine PhenotypeHFD - PhenotypeNC 0.000 0.651 4.522 Significant 0.651 tp_Creatinine Known
180 tp_Cytosine.tent. PhenotypeHFD - PhenotypeNC 0.011 0.446 1.948 Significant 0.446 tp_Cytosine.tent. Known
181 tp_D..Maltosec_364.09811_1.463 PhenotypeHFD - PhenotypeNC 0.000 3.785 7.659 Significant 3.785 tp_D..Maltosec_364.09811_1.463 Known
184 tp_DL.2.Aminocaprylic.U.FFFD._159.12591_1.519 PhenotypeHFD - PhenotypeNC 0.000 -0.838 4.417 Significant -0.838 tp_DL.2.Aminocaprylic.U.FFFD._159.12591_1.519 Known
185 tp_DL.Stachydrine PhenotypeHFD - PhenotypeNC 0.000 -4.225 11.798 Significant -4.225 tp_DL.Stachydrine Known
188 tp_gamma.L.glutamyl.L.tyrosine_310.11656_4.062 PhenotypeHFD - PhenotypeNC 0.050 0.558 1.303 Significant 0.558 tp_gamma.L.glutamyl.L.tyrosine_310.11656_4.062 Known
194 tp_L..Arginine PhenotypeHFD - PhenotypeNC 0.000 -0.703 4.098 Significant -0.703 tp_L..Arginine Known
197 tp_L.Carnitine PhenotypeHFD - PhenotypeNC 0.003 -0.328 2.475 Significant -0.328 tp_L.Carnitine Known
204 tp_L.Pipecolinic.acid.or.isomer. PhenotypeHFD - PhenotypeNC 0.010 0.690 2.016 Significant 0.690 tp_L.Pipecolinic.acid.or.isomer. Known
207 tp_N3.N4.Dimethyl.L.arginine PhenotypeHFD - PhenotypeNC 0.010 0.387 2.005 Significant 0.387 tp_N3.N4.Dimethyl.L.arginine Known
210 tp_Pantothenic.acid PhenotypeHFD - PhenotypeNC 0.011 0.316 1.948 Significant 0.316 tp_Pantothenic.acid Known
424 tp_Urocanic.acid.or.isomer. PhenotypeHFD - PhenotypeNC 0.010 0.556 2.001 Significant 0.556 tp_Urocanic.acid.or.isomer. Known
425 tp_Valine PhenotypeHFD - PhenotypeNC 0.042 0.540 1.374 Significant 0.540 tp_Valine Known
431 tp_X1.9Z.hexadecenoyl.sn.glycero.3.phosphocholine_493.31693_9.727 PhenotypeHFD - PhenotypeNC 0.010 -0.744 1.991 Significant -0.744 tp_X1.9Z.hexadecenoyl.sn.glycero.3.phosphocholine_493.31693_9.727 Known
432 tp_X1.arachidonoyl.sn.glycero.3.phosphocholine_543.33255_10.073 PhenotypeHFD - PhenotypeNC 0.045 0.622 1.343 Significant 0.622 tp_X1.arachidonoyl.sn.glycero.3.phosphocholine_543.33255_10.073 Known
436 tp_X1.Palmitoyl.2.hydroxy.sn.glycero.3.PE_453.2856_10.365 PhenotypeHFD - PhenotypeNC 0.011 -0.704 1.948 Significant -0.704 tp_X1.Palmitoyl.2.hydroxy.sn.glycero.3.PE_453.2856_10.365 Known
443 tp_X3.Aminobenzamide.or.isomer. PhenotypeHFD - PhenotypeNC 0.010 0.372 1.991 Significant 0.372 tp_X3.Aminobenzamide.or.isomer. Known
446 tp_X5.Nitro.o.toluidine.tent.or.isomer._152.05858_3.716 PhenotypeHFD - PhenotypeNC 0.036 0.448 1.449 Significant 0.448 tp_X5.Nitro.o.toluidine.tent.or.isomer._152.05858_3.716 Known
Metab variable value.x value.y p Significant lfc Label Unknown
73 pn_Unk_187.04567_1.248 PhenotypeHFD - PhenotypeNC 0.050 0.293 1.303 Significant 0.293 pn_Unk_187.04567_1.248 Unknown
76 pn_Unk_199.0569_1.178 PhenotypeHFD - PhenotypeNC 0.014 -0.314 1.849 Significant -0.314 pn_Unk_199.0569_1.178 Unknown
81 pn_Unk_226.03144_1.423 PhenotypeHFD - PhenotypeNC 0.000 -1.283 5.364 Significant -1.283 pn_Unk_226.03144_1.423 Unknown
89 pn_Unk_267.04429_1.177 PhenotypeHFD - PhenotypeNC 0.005 -0.397 2.304 Significant -0.397 pn_Unk_267.04429_1.177 Unknown
102 pn_Unk_295.96069_0.873 PhenotypeHFD - PhenotypeNC 0.050 -0.207 1.303 Significant -0.207 pn_Unk_295.96069_0.873 Unknown
105 pn_Unk_306.10631_5.003 PhenotypeHFD - PhenotypeNC 0.033 0.519 1.479 Significant 0.519 pn_Unk_306.10631_5.003 Unknown
113 pn_Unk_363.94815_0.875 PhenotypeHFD - PhenotypeNC 0.013 -0.250 1.886 Significant -0.250 pn_Unk_363.94815_0.875 Unknown
133 pn_Unk_533.88204_0.825 PhenotypeHFD - PhenotypeNC 0.048 -0.241 1.315 Significant -0.241 pn_Unk_533.88204_0.825 Unknown
140 pn_Unk_567.35351_10.425 PhenotypeHFD - PhenotypeNC 0.002 0.422 2.609 Significant 0.422 pn_Unk_567.35351_10.425 Unknown
142 pn_Unk_589.33795_10.139 PhenotypeHFD - PhenotypeNC 0.001 0.837 3.048 Significant 0.837 pn_Unk_589.33795_10.139 Unknown
242 tp_Unk_184.95252_1.15 PhenotypeHFD - PhenotypeNC 0.045 0.360 1.344 Significant 0.360 tp_Unk_184.95252_1.15 Unknown
244 tp_Unk_186.05044_1.342 PhenotypeHFD - PhenotypeNC 0.000 1.991 9.373 Significant 1.991 tp_Unk_186.05044_1.342 Unknown
257 tp_Unk_218.0756_1.148 PhenotypeHFD - PhenotypeNC 0.005 -0.892 2.304 Significant -0.892 tp_Unk_218.0756_1.148 Unknown
263 tp_Unk_234.04955_1.148 PhenotypeHFD - PhenotypeNC 0.000 -0.612 4.807 Significant -0.612 tp_Unk_234.04955_1.148 Unknown
285 tp_Unk_299.06784_1.026 PhenotypeHFD - PhenotypeNC 0.045 -0.259 1.344 Significant -0.259 tp_Unk_299.06784_1.026 Unknown
307 tp_Unk_361.8788_1.052 PhenotypeHFD - PhenotypeNC 0.005 0.336 2.317 Significant 0.336 tp_Unk_361.8788_1.052 Unknown
311 tp_Unk_366.14282_3.953 PhenotypeHFD - PhenotypeNC 0.011 0.503 1.940 Significant 0.503 tp_Unk_366.14282_3.953 Unknown
322 tp_Unk_407.91219_1.081 PhenotypeHFD - PhenotypeNC 0.017 -0.402 1.780 Significant -0.402 tp_Unk_407.91219_1.081 Unknown
340 tp_Unk_469.88265_1.072 PhenotypeHFD - PhenotypeNC 0.045 -0.245 1.343 Significant -0.245 tp_Unk_469.88265_1.072 Unknown
342 tp_Unk_473.82616_1.051 PhenotypeHFD - PhenotypeNC 0.006 0.390 2.241 Significant 0.390 tp_Unk_473.82616_1.051 Unknown
350 tp_Unk_504.26028_9.261 PhenotypeHFD - PhenotypeNC 0.048 0.835 1.315 Significant 0.835 tp_Unk_504.26028_9.261 Unknown
358 tp_Unk_523.82499_1.094 PhenotypeHFD - PhenotypeNC 0.050 0.222 1.303 Significant 0.222 tp_Unk_523.82499_1.094 Unknown
361 tp_Unk_543.33255_9.935 PhenotypeHFD - PhenotypeNC 0.012 0.842 1.905 Significant 0.842 tp_Unk_543.33255_9.935 Unknown
368 tp_Unk_562.26572_9.261 PhenotypeHFD - PhenotypeNC 0.002 0.944 2.729 Significant 0.944 tp_Unk_562.26572_9.261 Unknown
396 tp_Unk_727.78691_1.095 PhenotypeHFD - PhenotypeNC 0.045 0.171 1.343 Significant 0.171 tp_Unk_727.78691_1.095 Unknown
411 tp_Unk_833.73933_1.124 PhenotypeHFD - PhenotypeNC 0.035 0.957 1.456 Significant 0.957 tp_Unk_833.73933_1.124 Unknown