Code
library(readxl)
library(car)
library(multcomp)
library(ggplot2)
library(writexl)NB Project: Cell Death
Code
# Load the data
NB_data <- read_excel("Fold Change Calculations for Final Data_tidy.xlsx", sheet = 4)
data3 <- NB_data
# Display the first few rows of the imported data
head(data3)# A tibble: 6 × 4
Sample Gene Treatment cell_death
<dbl> <chr> <chr> <dbl>
1 1 C57 Control 4.4
2 2 C57 LPS 12.9
3 3 C57 ATP 8.4
4 4 C57 LPS+ATP 28.5
5 5 C57 NIG 1.2
6 6 C57 LPS+NIG 48.6Code
# Convert relevant columns to factors (Assuming 'Treatment' and 'Gene' are columns)
data3$Treatment <- factor(data3$Treatment, levels = c(
"Control", "ATP", "LPS", "LPS+ATP", "LPS+NIG", "NIG"))
data3$Gene <- as.factor(data3$Gene)
head(data3)# A tibble: 6 × 4
Sample Gene Treatment cell_death
<dbl> <fct> <fct> <dbl>
1 1 C57 Control 4.4
2 2 C57 LPS 12.9
3 3 C57 ATP 8.4
4 4 C57 LPS+ATP 28.5
5 5 C57 NIG 1.2
6 6 C57 LPS+NIG 48.6Code
# Perform a two-way ANOVA (Assuming 'cell_death' is the dependent variable)
anova_result <- aov(cell_death ~ Treatment * Gene, data = data3)
# Display the summary of ANOVA results
summary(anova_result) Df Sum Sq Mean Sq F value Pr(>F)
Treatment 5 34902 6980 427.83 < 2e-16 ***
Gene 3 2644 881 54.01 5.40e-16 ***
Treatment:Gene 15 3382 225 13.82 3.58e-13 ***
Residuals 52 848 16
---
Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
8 observations deleted due to missingnessCode
# Perform Tukey's HSD post-hoc test for multiple comparisons
tukey_result <- TukeyHSD(anova_result)
print(tukey_result) Tukey multiple comparisons of means
95% family-wise confidence level
Fit: aov(formula = cell_death ~ Treatment * Gene, data = data3)
$Treatment
diff lwr upr p adj
ATP-Control 9.044048e+00 4.342699 13.745396 0.0000085
LPS-Control 1.694048e+00 -3.007301 6.395396 0.8924712
LPS+ATP-Control 3.784725e+01 33.244299 42.450206 0.0000000
LPS+NIG-Control 5.636905e+01 51.667699 61.070396 0.0000000
NIG-Control 1.693407e+00 -2.909547 6.296360 0.8837820
LPS-ATP -7.350000e+00 -12.228820 -2.471180 0.0006076
LPS+ATP-ATP 2.880321e+01 24.019129 33.587282 0.0000000
LPS+NIG-ATP 4.732500e+01 42.446180 52.203820 0.0000000
NIG-ATP -7.350641e+00 -12.134718 -2.566564 0.0004531
LPS+ATP-LPS 3.615321e+01 31.369129 40.937282 0.0000000
LPS+NIG-LPS 5.467500e+01 49.796180 59.553820 0.0000000
NIG-LPS -6.410256e-04 -4.784718 4.783436 1.0000000
LPS+NIG-LPS+ATP 1.852179e+01 13.737718 23.305871 0.0000000
NIG-LPS+ATP -3.615385e+01 -40.841265 -31.466428 0.0000000
NIG-LPS+NIG -5.467564e+01 -59.459718 -49.891564 0.0000000
$Gene
diff lwr upr p adj
APOE4-APOE3 3.626186 0.1826258 7.0697464 0.0354192
C57-APOE3 -12.300000 -15.8735514 -8.7264486 0.0000000
NULL-APOE3 -3.377358 -6.9035760 0.1488591 0.0650924
C57-APOE4 -15.926186 -19.3697464 -12.4826258 0.0000000
NULL-APOE4 -7.003545 -10.3979588 -3.6091303 0.0000075
NULL-C57 8.922642 5.3964240 12.4488591 0.0000001
$`Treatment:Gene`
diff lwr upr p adj
ATP:APOE3-Control:APOE3 8.800000000 -3.8566619 21.4566619 0.5521507
LPS:APOE3-Control:APOE3 -1.800000000 -14.4566619 10.8566619 1.0000000
LPS+ATP:APOE3-Control:APOE3 31.533333333 18.8766714 44.1899953 0.0000000
LPS+NIG:APOE3-Control:APOE3 49.300000000 36.6433381 61.9566619 0.0000000
NIG:APOE3-Control:APOE3 -0.766666667 -13.4233286 11.8899953 1.0000000
Control:APOE4-Control:APOE3 -5.933333333 -17.7725565 5.9058898 0.9522620
ATP:APOE4-Control:APOE3 7.566666667 -5.0899953 20.2233286 0.8019927
LPS:APOE4-Control:APOE3 -7.000000000 -19.6566619 5.6566619 0.8874077
LPS+ATP:APOE4-Control:APOE3 51.866666667 40.0274435 63.7058898 0.0000000
LPS+NIG:APOE4-Control:APOE3 64.433333333 51.7766714 77.0899953 0.0000000
NIG:APOE4-Control:APOE3 -2.908333333 -14.7475565 8.9308898 0.9999984
Control:C57-Control:APOE3 -9.833333333 -22.4899953 2.8233286 0.3428141
ATP:C57-Control:APOE3 -5.600000000 -18.2566619 7.0566619 0.9871084
LPS:C57-Control:APOE3 -6.733333333 -19.3899953 5.9233286 0.9181652
LPS+ATP:C57-Control:APOE3 14.766666667 2.1100047 27.4233286 0.0078401
LPS+NIG:C57-Control:APOE3 27.000000000 14.3433381 39.6566619 0.0000000
NIG:C57-Control:APOE3 -6.333333333 -18.9899953 6.3233286 0.9529505
Control:NULL-Control:APOE3 -7.758333333 -19.5975565 4.0808898 0.6606363
ATP:NULL-Control:APOE3 1.333333333 -11.3233286 13.9899953 1.0000000
LPS:NULL-Control:APOE3 -1.766666667 -14.4233286 10.8899953 1.0000000
LPS+ATP:NULL-Control:APOE3 22.466666667 9.8100047 35.1233286 0.0000025
LPS+NIG:NULL-Control:APOE3 60.666666667 48.0100047 73.3233286 0.0000000
NIG:NULL-Control:APOE3 -7.766666667 -20.4233286 4.8899953 0.7660162
LPS:APOE3-ATP:APOE3 -10.600000000 -23.2566619 2.0566619 0.2204672
LPS+ATP:APOE3-ATP:APOE3 22.733333333 10.0766714 35.3899953 0.0000019
LPS+NIG:APOE3-ATP:APOE3 40.500000000 27.8433381 53.1566619 0.0000000
NIG:APOE3-ATP:APOE3 -9.566666667 -22.2233286 3.0899953 0.3930005
Control:APOE4-ATP:APOE3 -14.733333333 -26.5725565 -2.8941102 0.0030525
ATP:APOE4-ATP:APOE3 -1.233333333 -13.8899953 11.4233286 1.0000000
LPS:APOE4-ATP:APOE3 -15.800000000 -28.4566619 -3.1433381 0.0029082
LPS+ATP:APOE4-ATP:APOE3 43.066666667 31.2274435 54.9058898 0.0000000
LPS+NIG:APOE4-ATP:APOE3 55.633333333 42.9766714 68.2899953 0.0000000
NIG:APOE4-ATP:APOE3 -11.708333333 -23.5475565 0.1308898 0.0559583
Control:C57-ATP:APOE3 -18.633333333 -31.2899953 -5.9766714 0.0001597
ATP:C57-ATP:APOE3 -14.400000000 -27.0566619 -1.7433381 0.0110174
LPS:C57-ATP:APOE3 -15.533333333 -28.1899953 -2.8766714 0.0037725
LPS+ATP:C57-ATP:APOE3 5.966666667 -6.6899953 18.6233286 0.9741134
LPS+NIG:C57-ATP:APOE3 18.200000000 5.5433381 30.8566619 0.0002522
NIG:C57-ATP:APOE3 -15.133333333 -27.7899953 -2.4766714 0.0055434
Control:NULL-ATP:APOE3 -16.558333333 -28.3975565 -4.7191102 0.0004244
ATP:NULL-ATP:APOE3 -7.466666667 -20.1233286 5.1899953 0.8189295
LPS:NULL-ATP:APOE3 -10.566666667 -23.2233286 2.0899953 0.2250596
LPS+ATP:NULL-ATP:APOE3 13.666666667 1.0100047 26.3233286 0.0212971
LPS+NIG:NULL-ATP:APOE3 51.866666667 39.2100047 64.5233286 0.0000000
NIG:NULL-ATP:APOE3 -16.566666667 -29.2233286 -3.9100047 0.0013567
LPS+ATP:APOE3-LPS:APOE3 33.333333333 20.6766714 45.9899953 0.0000000
LPS+NIG:APOE3-LPS:APOE3 51.100000000 38.4433381 63.7566619 0.0000000
NIG:APOE3-LPS:APOE3 1.033333333 -11.6233286 13.6899953 1.0000000
Control:APOE4-LPS:APOE3 -4.133333333 -15.9725565 7.7058898 0.9994343
ATP:APOE4-LPS:APOE3 9.366666667 -3.2899953 22.0233286 0.4327662
LPS:APOE4-LPS:APOE3 -5.200000000 -17.8566619 7.4566619 0.9947187
LPS+ATP:APOE4-LPS:APOE3 53.666666667 41.8274435 65.5058898 0.0000000
LPS+NIG:APOE4-LPS:APOE3 66.233333333 53.5766714 78.8899953 0.0000000
NIG:APOE4-LPS:APOE3 -1.108333333 -12.9475565 10.7308898 1.0000000
Control:C57-LPS:APOE3 -8.033333333 -20.6899953 4.6233286 0.7143047
ATP:C57-LPS:APOE3 -3.800000000 -16.4566619 8.8566619 0.9999470
LPS:C57-LPS:APOE3 -4.933333333 -17.5899953 7.7233286 0.9973315
LPS+ATP:C57-LPS:APOE3 16.566666667 3.9100047 29.2233286 0.0013567
LPS+NIG:C57-LPS:APOE3 28.800000000 16.1433381 41.4566619 0.0000000
NIG:C57-LPS:APOE3 -4.533333333 -17.1899953 8.1233286 0.9991772
Control:NULL-LPS:APOE3 -5.958333333 -17.7975565 5.8808898 0.9503210
ATP:NULL-LPS:APOE3 3.133333333 -9.5233286 15.7899953 0.9999982
LPS:NULL-LPS:APOE3 0.033333333 -12.6233286 12.6899953 1.0000000
LPS+ATP:NULL-LPS:APOE3 24.266666667 11.6100047 36.9233286 0.0000003
LPS+NIG:NULL-LPS:APOE3 62.466666667 49.8100047 75.1233286 0.0000000
NIG:NULL-LPS:APOE3 -5.966666667 -18.6233286 6.6899953 0.9741134
LPS+NIG:APOE3-LPS+ATP:APOE3 17.766666667 5.1100047 30.4233286 0.0003967
NIG:APOE3-LPS+ATP:APOE3 -32.300000000 -44.9566619 -19.6433381 0.0000000
Control:APOE4-LPS+ATP:APOE3 -37.466666667 -49.3058898 -25.6274435 0.0000000
ATP:APOE4-LPS+ATP:APOE3 -23.966666667 -36.6233286 -11.3100047 0.0000005
LPS:APOE4-LPS+ATP:APOE3 -38.533333333 -51.1899953 -25.8766714 0.0000000
LPS+ATP:APOE4-LPS+ATP:APOE3 20.333333333 8.4941102 32.1725565 0.0000056
LPS+NIG:APOE4-LPS+ATP:APOE3 32.900000000 20.2433381 45.5566619 0.0000000
NIG:APOE4-LPS+ATP:APOE3 -34.441666667 -46.2808898 -22.6024435 0.0000000
Control:C57-LPS+ATP:APOE3 -41.366666667 -54.0233286 -28.7100047 0.0000000
ATP:C57-LPS+ATP:APOE3 -37.133333333 -49.7899953 -24.4766714 0.0000000
LPS:C57-LPS+ATP:APOE3 -38.266666667 -50.9233286 -25.6100047 0.0000000
LPS+ATP:C57-LPS+ATP:APOE3 -16.766666667 -29.4233286 -4.1100047 0.0011084
LPS+NIG:C57-LPS+ATP:APOE3 -4.533333333 -17.1899953 8.1233286 0.9991772
NIG:C57-LPS+ATP:APOE3 -37.866666667 -50.5233286 -25.2100047 0.0000000
Control:NULL-LPS+ATP:APOE3 -39.291666667 -51.1308898 -27.4524435 0.0000000
ATP:NULL-LPS+ATP:APOE3 -30.200000000 -42.8566619 -17.5433381 0.0000000
LPS:NULL-LPS+ATP:APOE3 -33.300000000 -45.9566619 -20.6433381 0.0000000
LPS+ATP:NULL-LPS+ATP:APOE3 -9.066666667 -21.7233286 3.5899953 0.4950679
LPS+NIG:NULL-LPS+ATP:APOE3 29.133333333 16.4766714 41.7899953 0.0000000
NIG:NULL-LPS+ATP:APOE3 -39.300000000 -51.9566619 -26.6433381 0.0000000
NIG:APOE3-LPS+NIG:APOE3 -50.066666667 -62.7233286 -37.4100047 0.0000000
Control:APOE4-LPS+NIG:APOE3 -55.233333333 -67.0725565 -43.3941102 0.0000000
ATP:APOE4-LPS+NIG:APOE3 -41.733333333 -54.3899953 -29.0766714 0.0000000
LPS:APOE4-LPS+NIG:APOE3 -56.300000000 -68.9566619 -43.6433381 0.0000000
LPS+ATP:APOE4-LPS+NIG:APOE3 2.566666667 -9.2725565 14.4058898 0.9999999
LPS+NIG:APOE4-LPS+NIG:APOE3 15.133333333 2.4766714 27.7899953 0.0055434
NIG:APOE4-LPS+NIG:APOE3 -52.208333333 -64.0475565 -40.3691102 0.0000000
Control:C57-LPS+NIG:APOE3 -59.133333333 -71.7899953 -46.4766714 0.0000000
ATP:C57-LPS+NIG:APOE3 -54.900000000 -67.5566619 -42.2433381 0.0000000
LPS:C57-LPS+NIG:APOE3 -56.033333333 -68.6899953 -43.3766714 0.0000000
LPS+ATP:C57-LPS+NIG:APOE3 -34.533333333 -47.1899953 -21.8766714 0.0000000
LPS+NIG:C57-LPS+NIG:APOE3 -22.300000000 -34.9566619 -9.6433381 0.0000030
NIG:C57-LPS+NIG:APOE3 -55.633333333 -68.2899953 -42.9766714 0.0000000
Control:NULL-LPS+NIG:APOE3 -57.058333333 -68.8975565 -45.2191102 0.0000000
ATP:NULL-LPS+NIG:APOE3 -47.966666667 -60.6233286 -35.3100047 0.0000000
LPS:NULL-LPS+NIG:APOE3 -51.066666667 -63.7233286 -38.4100047 0.0000000
LPS+ATP:NULL-LPS+NIG:APOE3 -26.833333333 -39.4899953 -14.1766714 0.0000000
LPS+NIG:NULL-LPS+NIG:APOE3 11.366666667 -1.2899953 24.0233286 0.1328085
NIG:NULL-LPS+NIG:APOE3 -57.066666667 -69.7233286 -44.4100047 0.0000000
Control:APOE4-NIG:APOE3 -5.166666667 -17.0058898 6.6725565 0.9890103
ATP:APOE4-NIG:APOE3 8.333333333 -4.3233286 20.9899953 0.6523457
LPS:APOE4-NIG:APOE3 -6.233333333 -18.8899953 6.4233286 0.9596597
LPS+ATP:APOE4-NIG:APOE3 52.633333333 40.7941102 64.4725565 0.0000000
LPS+NIG:APOE4-NIG:APOE3 65.200000000 52.5433381 77.8566619 0.0000000
NIG:APOE4-NIG:APOE3 -2.141666667 -13.9808898 9.6975565 1.0000000
Control:C57-NIG:APOE3 -9.066666667 -21.7233286 3.5899953 0.4950679
ATP:C57-NIG:APOE3 -4.833333333 -17.4899953 7.8233286 0.9979753
LPS:C57-NIG:APOE3 -5.966666667 -18.6233286 6.6899953 0.9741134
LPS+ATP:C57-NIG:APOE3 15.533333333 2.8766714 28.1899953 0.0037725
LPS+NIG:C57-NIG:APOE3 27.766666667 15.1100047 40.4233286 0.0000000
NIG:C57-NIG:APOE3 -5.566666667 -18.2233286 7.0899953 0.9879659
Control:NULL-NIG:APOE3 -6.991666667 -18.8308898 4.8475565 0.8176453
ATP:NULL-NIG:APOE3 2.100000000 -10.5566619 14.7566619 1.0000000
LPS:NULL-NIG:APOE3 -1.000000000 -13.6566619 11.6566619 1.0000000
LPS+ATP:NULL-NIG:APOE3 23.233333333 10.5766714 35.8899953 0.0000011
LPS+NIG:NULL-NIG:APOE3 61.433333333 48.7766714 74.0899953 0.0000000
NIG:NULL-NIG:APOE3 -7.000000000 -19.6566619 5.6566619 0.8874077
ATP:APOE4-Control:APOE4 13.500000000 1.6607769 25.3392231 0.0106969
LPS:APOE4-Control:APOE4 -1.066666667 -12.9058898 10.7725565 1.0000000
LPS+ATP:APOE4-Control:APOE4 57.800000000 46.8390092 68.7609908 0.0000000
LPS+NIG:APOE4-Control:APOE4 70.366666667 58.5274435 82.2058898 0.0000000
NIG:APOE4-Control:APOE4 3.025000000 -7.9359908 13.9859908 0.9999873
Control:C57-Control:APOE4 -3.900000000 -15.7392231 7.9392231 0.9997655
ATP:C57-Control:APOE4 0.333333333 -11.5058898 12.1725565 1.0000000
LPS:C57-Control:APOE4 -0.800000000 -12.6392231 11.0392231 1.0000000
LPS+ATP:C57-Control:APOE4 20.700000000 8.8607769 32.5392231 0.0000036
LPS+NIG:C57-Control:APOE4 32.933333333 21.0941102 44.7725565 0.0000000
NIG:C57-Control:APOE4 -0.400000000 -12.2392231 11.4392231 1.0000000
Control:NULL-Control:APOE4 -1.825000000 -12.7859908 9.1359908 1.0000000
ATP:NULL-Control:APOE4 7.266666667 -4.5725565 19.1058898 0.7656942
LPS:NULL-Control:APOE4 4.166666667 -7.6725565 16.0058898 0.9993632
LPS+ATP:NULL-Control:APOE4 28.400000000 16.5607769 40.2392231 0.0000000
LPS+NIG:NULL-Control:APOE4 66.600000000 54.7607769 78.4392231 0.0000000
NIG:NULL-Control:APOE4 -1.833333333 -13.6725565 10.0058898 1.0000000
LPS:APOE4-ATP:APOE4 -14.566666667 -27.2233286 -1.9100047 0.0094466
LPS+ATP:APOE4-ATP:APOE4 44.300000000 32.4607769 56.1392231 0.0000000
LPS+NIG:APOE4-ATP:APOE4 56.866666667 44.2100047 69.5233286 0.0000000
NIG:APOE4-ATP:APOE4 -10.475000000 -22.3142231 1.3642231 0.1492186
Control:C57-ATP:APOE4 -17.400000000 -30.0566619 -4.7433381 0.0005799
ATP:C57-ATP:APOE4 -13.166666667 -25.8233286 -0.5100047 0.0327738
LPS:C57-ATP:APOE4 -14.300000000 -26.9566619 -1.6433381 0.0120744
LPS+ATP:C57-ATP:APOE4 7.200000000 -5.4566619 19.8566619 0.8602843
LPS+NIG:C57-ATP:APOE4 19.433333333 6.7766714 32.0899953 0.0000681
NIG:C57-ATP:APOE4 -13.900000000 -26.5566619 -1.2433381 0.0173249
Control:NULL-ATP:APOE4 -15.325000000 -27.1642231 -3.4857769 0.0016314
ATP:NULL-ATP:APOE4 -6.233333333 -18.8899953 6.4233286 0.9596597
LPS:NULL-ATP:APOE4 -9.333333333 -21.9899953 3.3233286 0.4395469
LPS+ATP:NULL-ATP:APOE4 14.900000000 2.2433381 27.5566619 0.0069165
LPS+NIG:NULL-ATP:APOE4 53.100000000 40.4433381 65.7566619 0.0000000
NIG:NULL-ATP:APOE4 -15.333333333 -27.9899953 -2.6766714 0.0045769
LPS+ATP:APOE4-LPS:APOE4 58.866666667 47.0274435 70.7058898 0.0000000
LPS+NIG:APOE4-LPS:APOE4 71.433333333 58.7766714 84.0899953 0.0000000
NIG:APOE4-LPS:APOE4 4.091666667 -7.7475565 15.9308898 0.9995134
Control:C57-LPS:APOE4 -2.833333333 -15.4899953 9.8233286 0.9999997
ATP:C57-LPS:APOE4 1.400000000 -11.2566619 14.0566619 1.0000000
LPS:C57-LPS:APOE4 0.266666667 -12.3899953 12.9233286 1.0000000
LPS+ATP:C57-LPS:APOE4 21.766666667 9.1100047 34.4233286 0.0000054
LPS+NIG:C57-LPS:APOE4 34.000000000 21.3433381 46.6566619 0.0000000
NIG:C57-LPS:APOE4 0.666666667 -11.9899953 13.3233286 1.0000000
Control:NULL-LPS:APOE4 -0.758333333 -12.5975565 11.0808898 1.0000000
ATP:NULL-LPS:APOE4 8.333333333 -4.3233286 20.9899953 0.6523457
LPS:NULL-LPS:APOE4 5.233333333 -7.4233286 17.8899953 0.9942780
LPS+ATP:NULL-LPS:APOE4 29.466666667 16.8100047 42.1233286 0.0000000
LPS+NIG:NULL-LPS:APOE4 67.666666667 55.0100047 80.3233286 0.0000000
NIG:NULL-LPS:APOE4 -0.766666667 -13.4233286 11.8899953 1.0000000
LPS+NIG:APOE4-LPS+ATP:APOE4 12.566666667 0.7274435 24.4058898 0.0260716
NIG:APOE4-LPS+ATP:APOE4 -54.775000000 -65.7359908 -43.8140092 0.0000000
Control:C57-LPS+ATP:APOE4 -61.700000000 -73.5392231 -49.8607769 0.0000000
ATP:C57-LPS+ATP:APOE4 -57.466666667 -69.3058898 -45.6274435 0.0000000
LPS:C57-LPS+ATP:APOE4 -58.600000000 -70.4392231 -46.7607769 0.0000000
LPS+ATP:C57-LPS+ATP:APOE4 -37.100000000 -48.9392231 -25.2607769 0.0000000
LPS+NIG:C57-LPS+ATP:APOE4 -24.866666667 -36.7058898 -13.0274435 0.0000000
NIG:C57-LPS+ATP:APOE4 -58.200000000 -70.0392231 -46.3607769 0.0000000
Control:NULL-LPS+ATP:APOE4 -59.625000000 -70.5859908 -48.6640092 0.0000000
ATP:NULL-LPS+ATP:APOE4 -50.533333333 -62.3725565 -38.6941102 0.0000000
LPS:NULL-LPS+ATP:APOE4 -53.633333333 -65.4725565 -41.7941102 0.0000000
LPS+ATP:NULL-LPS+ATP:APOE4 -29.400000000 -41.2392231 -17.5607769 0.0000000
LPS+NIG:NULL-LPS+ATP:APOE4 8.800000000 -3.0392231 20.6392231 0.4244968
NIG:NULL-LPS+ATP:APOE4 -59.633333333 -71.4725565 -47.7941102 0.0000000
NIG:APOE4-LPS+NIG:APOE4 -67.341666667 -79.1808898 -55.5024435 0.0000000
Control:C57-LPS+NIG:APOE4 -74.266666667 -86.9233286 -61.6100047 0.0000000
ATP:C57-LPS+NIG:APOE4 -70.033333333 -82.6899953 -57.3766714 0.0000000
LPS:C57-LPS+NIG:APOE4 -71.166666667 -83.8233286 -58.5100047 0.0000000
LPS+ATP:C57-LPS+NIG:APOE4 -49.666666667 -62.3233286 -37.0100047 0.0000000
LPS+NIG:C57-LPS+NIG:APOE4 -37.433333333 -50.0899953 -24.7766714 0.0000000
NIG:C57-LPS+NIG:APOE4 -70.766666667 -83.4233286 -58.1100047 0.0000000
Control:NULL-LPS+NIG:APOE4 -72.191666667 -84.0308898 -60.3524435 0.0000000
ATP:NULL-LPS+NIG:APOE4 -63.100000000 -75.7566619 -50.4433381 0.0000000
LPS:NULL-LPS+NIG:APOE4 -66.200000000 -78.8566619 -53.5433381 0.0000000
LPS+ATP:NULL-LPS+NIG:APOE4 -41.966666667 -54.6233286 -29.3100047 0.0000000
LPS+NIG:NULL-LPS+NIG:APOE4 -3.766666667 -16.4233286 8.8899953 0.9999542
NIG:NULL-LPS+NIG:APOE4 -72.200000000 -84.8566619 -59.5433381 0.0000000
Control:C57-NIG:APOE4 -6.925000000 -18.7642231 4.9142231 0.8292973
ATP:C57-NIG:APOE4 -2.691666667 -14.5308898 9.1475565 0.9999996
LPS:C57-NIG:APOE4 -3.825000000 -15.6642231 8.0142231 0.9998268
LPS+ATP:C57-NIG:APOE4 17.675000000 5.8357769 29.5142231 0.0001209
LPS+NIG:C57-NIG:APOE4 29.908333333 18.0691102 41.7475565 0.0000000
NIG:C57-NIG:APOE4 -3.425000000 -15.2642231 8.4142231 0.9999715
Control:NULL-NIG:APOE4 -4.850000000 -15.8109908 6.1109908 0.9871006
ATP:NULL-NIG:APOE4 4.241666667 -7.5975565 16.0808898 0.9991740
LPS:NULL-NIG:APOE4 1.141666667 -10.6975565 12.9808898 1.0000000
LPS+ATP:NULL-NIG:APOE4 25.375000000 13.5357769 37.2142231 0.0000000
LPS+NIG:NULL-NIG:APOE4 63.575000000 51.7357769 75.4142231 0.0000000
NIG:NULL-NIG:APOE4 -4.858333333 -16.6975565 6.9808898 0.9947978
ATP:C57-Control:C57 4.233333333 -8.4233286 16.8899953 0.9997034
LPS:C57-Control:C57 3.100000000 -9.5566619 15.7566619 0.9999985
LPS+ATP:C57-Control:C57 24.600000000 11.9433381 37.2566619 0.0000002
LPS+NIG:C57-Control:C57 36.833333333 24.1766714 49.4899953 0.0000000
NIG:C57-Control:C57 3.500000000 -9.1566619 16.1566619 0.9999868
Control:NULL-Control:C57 2.075000000 -9.7642231 13.9142231 1.0000000
ATP:NULL-Control:C57 11.166666667 -1.4899953 23.8233286 0.1524665
LPS:NULL-Control:C57 8.066666667 -4.5899953 20.7233286 0.7075894
LPS+ATP:NULL-Control:C57 32.300000000 19.6433381 44.9566619 0.0000000
LPS+NIG:NULL-Control:C57 70.500000000 57.8433381 83.1566619 0.0000000
NIG:NULL-Control:C57 2.066666667 -10.5899953 14.7233286 1.0000000
LPS:C57-ATP:C57 -1.133333333 -13.7899953 11.5233286 1.0000000
LPS+ATP:C57-ATP:C57 20.366666667 7.7100047 33.0233286 0.0000249
LPS+NIG:C57-ATP:C57 32.600000000 19.9433381 45.2566619 0.0000000
NIG:C57-ATP:C57 -0.733333333 -13.3899953 11.9233286 1.0000000
Control:NULL-ATP:C57 -2.158333333 -13.9975565 9.6808898 1.0000000
ATP:NULL-ATP:C57 6.933333333 -5.7233286 19.5899953 0.8956791
LPS:NULL-ATP:C57 3.833333333 -8.8233286 16.4899953 0.9999388
LPS+ATP:NULL-ATP:C57 28.066666667 15.4100047 40.7233286 0.0000000
LPS+NIG:NULL-ATP:C57 66.266666667 53.6100047 78.9233286 0.0000000
NIG:NULL-ATP:C57 -2.166666667 -14.8233286 10.4899953 1.0000000
LPS+ATP:C57-LPS:C57 21.500000000 8.8433381 34.1566619 0.0000072
LPS+NIG:C57-LPS:C57 33.733333333 21.0766714 46.3899953 0.0000000
NIG:C57-LPS:C57 0.400000000 -12.2566619 13.0566619 1.0000000
Control:NULL-LPS:C57 -1.025000000 -12.8642231 10.8142231 1.0000000
ATP:NULL-LPS:C57 8.066666667 -4.5899953 20.7233286 0.7075894
LPS:NULL-LPS:C57 4.966666667 -7.6899953 17.6233286 0.9970817
LPS+ATP:NULL-LPS:C57 29.200000000 16.5433381 41.8566619 0.0000000
LPS+NIG:NULL-LPS:C57 67.400000000 54.7433381 80.0566619 0.0000000
NIG:NULL-LPS:C57 -1.033333333 -13.6899953 11.6233286 1.0000000
LPS+NIG:C57-LPS+ATP:C57 12.233333333 -0.4233286 24.8899953 0.0699641
NIG:C57-LPS+ATP:C57 -21.100000000 -33.7566619 -8.4433381 0.0000112
Control:NULL-LPS+ATP:C57 -22.525000000 -34.3642231 -10.6857769 0.0000004
ATP:NULL-LPS+ATP:C57 -13.433333333 -26.0899953 -0.7766714 0.0260941
LPS:NULL-LPS+ATP:C57 -16.533333333 -29.1899953 -3.8766714 0.0014030
LPS+ATP:NULL-LPS+ATP:C57 7.700000000 -4.9566619 20.3566619 0.7783031
LPS+NIG:NULL-LPS+ATP:C57 45.900000000 33.2433381 58.5566619 0.0000000
NIG:NULL-LPS+ATP:C57 -22.533333333 -35.1899953 -9.8766714 0.0000023
NIG:C57-LPS+NIG:C57 -33.333333333 -45.9899953 -20.6766714 0.0000000
Control:NULL-LPS+NIG:C57 -34.758333333 -46.5975565 -22.9191102 0.0000000
ATP:NULL-LPS+NIG:C57 -25.666666667 -38.3233286 -13.0100047 0.0000001
LPS:NULL-LPS+NIG:C57 -28.766666667 -41.4233286 -16.1100047 0.0000000
LPS+ATP:NULL-LPS+NIG:C57 -4.533333333 -17.1899953 8.1233286 0.9991772
LPS+NIG:NULL-LPS+NIG:C57 33.666666667 21.0100047 46.3233286 0.0000000
NIG:NULL-LPS+NIG:C57 -34.766666667 -47.4233286 -22.1100047 0.0000000
Control:NULL-NIG:C57 -1.425000000 -13.2642231 10.4142231 1.0000000
ATP:NULL-NIG:C57 7.666666667 -4.9899953 20.3233286 0.7843389
LPS:NULL-NIG:C57 4.566666667 -8.0899953 17.2233286 0.9990854
LPS+ATP:NULL-NIG:C57 28.800000000 16.1433381 41.4566619 0.0000000
LPS+NIG:NULL-NIG:C57 67.000000000 54.3433381 79.6566619 0.0000000
NIG:NULL-NIG:C57 -1.433333333 -14.0899953 11.2233286 1.0000000
ATP:NULL-Control:NULL 9.091666667 -2.7475565 20.9308898 0.3638261
LPS:NULL-Control:NULL 5.991666667 -5.8475565 17.8308898 0.9476465
LPS+ATP:NULL-Control:NULL 30.225000000 18.3857769 42.0642231 0.0000000
LPS+NIG:NULL-Control:NULL 68.425000000 56.5857769 80.2642231 0.0000000
NIG:NULL-Control:NULL -0.008333333 -11.8475565 11.8308898 1.0000000
LPS:NULL-ATP:NULL -3.100000000 -15.7566619 9.5566619 0.9999985
LPS+ATP:NULL-ATP:NULL 21.133333333 8.4766714 33.7899953 0.0000108
LPS+NIG:NULL-ATP:NULL 59.333333333 46.6766714 71.9899953 0.0000000
NIG:NULL-ATP:NULL -9.100000000 -21.7566619 3.5566619 0.4880210
LPS+ATP:NULL-LPS:NULL 24.233333333 11.5766714 36.8899953 0.0000004
LPS+NIG:NULL-LPS:NULL 62.433333333 49.7766714 75.0899953 0.0000000
NIG:NULL-LPS:NULL -6.000000000 -18.6566619 6.6566619 0.9725615
LPS+NIG:NULL-LPS+ATP:NULL 38.200000000 25.5433381 50.8566619 0.0000000
NIG:NULL-LPS+ATP:NULL -30.233333333 -42.8899953 -17.5766714 0.0000000
NIG:NULL-LPS+NIG:NULL -68.433333333 -81.0899953 -55.7766714 0.0000000