Code
library(readxl)
library(car)
library(multcomp)
library(ggplot2)
library(writexl)NB Project: IL-1b
Code
# Load the data
NB_data <- read_excel("Fold Change Calculations for Final Data_tidy.xlsx", sheet = 3)
data3 <- NB_data
# Display the first few rows of the imported data
head(data3)# A tibble: 6 × 4
Sample Gene Treatment IL_1b
<dbl> <chr> <chr> <dbl>
1 1 C57 Control 1.26
2 2 C57 LPS 4.48
3 3 C57 ATP 17.5
4 4 C57 LPS+ATP 114.
5 5 C57 NIG 27.0
6 6 C57 LPS+NIG 1810. Code
# Convert relevant columns to factors (Assuming 'Treatment' and 'Gene' are columns)
data3$Treatment <- factor(data3$Treatment, levels = c(
"Control", "ATP", "LPS", "LPS+ATP", "LPS+NIG", "NIG"))
data3$Gene <- as.factor(data3$Gene)
head(data3)# A tibble: 6 × 4
Sample Gene Treatment IL_1b
<dbl> <fct> <fct> <dbl>
1 1 C57 Control 1.26
2 2 C57 LPS 4.48
3 3 C57 ATP 17.5
4 4 C57 LPS+ATP 114.
5 5 C57 NIG 27.0
6 6 C57 LPS+NIG 1810. Code
# Perform a two-way ANOVA (Assuming 'JESS_Active_Caspase_1' is the dependent variable)
anova_result <- aov(IL_1b ~ Treatment * Gene, data = data3)
# Display the summary of ANOVA results
summary(anova_result) Df Sum Sq Mean Sq F value Pr(>F)
Treatment 5 27995188 5599038 159.83 < 2e-16 ***
Gene 3 1234290 411430 11.74 3.58e-06 ***
Treatment:Gene 15 5291281 352752 10.07 1.88e-11 ***
Residuals 61 2136891 35031
---
Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
11 observations deleted due to missingnessCode
# Perform Tukey's HSD post-hoc test for multiple comparisons
tukey_result <- TukeyHSD(anova_result)
print(tukey_result) Tukey multiple comparisons of means
95% family-wise confidence level
Fit: aov(formula = IL_1b ~ Treatment * Gene, data = data3)
$Treatment
diff lwr upr p adj
ATP-Control 3.489423 -202.1327 209.1115 1.0000000
LPS-Control 4.526346 -201.0958 210.1485 0.9999998
LPS+ATP-Control 865.570357 664.0406 1067.1001 0.0000000
LPS+NIG-Control 1437.608500 1239.6939 1635.5231 0.0000000
NIG-Control 5.911786 -195.6179 207.4415 0.9999993
LPS-ATP 1.036923 -214.9591 217.0330 1.0000000
LPS+ATP-ATP 862.080934 649.9770 1074.1849 0.0000000
LPS+NIG-ATP 1434.119077 1225.4471 1642.7911 0.0000000
NIG-ATP 2.422363 -209.6816 214.5263 1.0000000
LPS+ATP-LPS 861.044011 648.9401 1073.1479 0.0000000
LPS+NIG-LPS 1433.082154 1224.4101 1641.7542 0.0000000
NIG-LPS 1.385440 -210.7185 213.4894 1.0000000
LPS+NIG-LPS+ATP 572.038143 367.3975 776.6788 0.0000000
NIG-LPS+ATP -859.658571 -1067.7976 -651.5196 0.0000000
NIG-LPS+NIG -1431.696714 -1636.3373 -1227.0561 0.0000000
$Gene
diff lwr upr p adj
APOE4-APOE3 219.45996 70.40503 368.51488 0.0014023
C57-APOE3 -27.71842 -180.45426 125.01743 0.9633811
NULL-APOE3 -100.48643 -251.30538 50.33252 0.3025286
C57-APOE4 -247.17838 -399.91422 -94.44253 0.0003899
NULL-APOE4 -319.94639 -470.76533 -169.12744 0.0000032
NULL-C57 -72.76801 -227.22585 81.68983 0.6014165
$`Treatment:Gene`
diff lwr upr p adj
ATP:APOE3-Control:APOE3 0.7500000 -503.20523 504.70523 1.0000000
LPS:APOE3-Control:APOE3 2.7750000 -541.55886 547.10886 1.0000000
LPS+ATP:APOE3-Control:APOE3 1081.9416667 537.60781 1626.27553 0.0000001
LPS+NIG:APOE3-Control:APOE3 1153.3000000 649.34477 1657.25523 0.0000000
NIG:APOE3-Control:APOE3 3.6750000 -500.28023 507.63023 1.0000000
Control:APOE4-Control:APOE3 0.9750000 -502.98023 504.93023 1.0000000
ATP:APOE4-Control:APOE3 2.0750000 -542.25886 546.40886 1.0000000
LPS:APOE4-Control:APOE3 11.6750000 -532.65886 556.00886 1.0000000
LPS+ATP:APOE4-Control:APOE3 1673.8000000 1169.84477 2177.75523 0.0000000
LPS+NIG:APOE4-Control:APOE3 1706.4750000 1202.51977 2210.43023 0.0000000
NIG:APOE4-Control:APOE3 2.2500000 -501.70523 506.20523 1.0000000
Control:C57-Control:APOE3 -0.6575000 -504.61273 503.29773 1.0000000
ATP:C57-Control:APOE3 11.0183333 -533.31553 555.35219 1.0000000
LPS:C57-Control:APOE3 3.5450000 -500.41023 507.50023 1.0000000
LPS+ATP:C57-Control:APOE3 102.2283333 -442.10553 646.56219 1.0000000
LPS+NIG:C57-Control:APOE3 1841.0950000 1296.76114 2385.42886 0.0000000
NIG:C57-Control:APOE3 20.0950000 -524.23886 564.42886 1.0000000
Control:NULL-Control:APOE3 8.1725000 -495.78273 512.12773 1.0000000
ATP:NULL-Control:APOE3 10.2250000 -534.10886 554.55886 1.0000000
LPS:NULL-Control:APOE3 9.6350000 -534.69886 553.96886 1.0000000
LPS+ATP:NULL-Control:APOE3 474.9975000 -28.95773 978.95273 0.0893318
LPS+NIG:NULL-Control:APOE3 1158.3950000 654.43977 1662.35023 0.0000000
NIG:NULL-Control:APOE3 9.4983333 -534.83553 553.83219 1.0000000
LPS:APOE3-ATP:APOE3 2.0250000 -542.30886 546.35886 1.0000000
LPS+ATP:APOE3-ATP:APOE3 1081.1916667 536.85781 1625.52553 0.0000001
LPS+NIG:APOE3-ATP:APOE3 1152.5500000 648.59477 1656.50523 0.0000000
NIG:APOE3-ATP:APOE3 2.9250000 -501.03023 506.88023 1.0000000
Control:APOE4-ATP:APOE3 0.2250000 -503.73023 504.18023 1.0000000
ATP:APOE4-ATP:APOE3 1.3250000 -543.00886 545.65886 1.0000000
LPS:APOE4-ATP:APOE3 10.9250000 -533.40886 555.25886 1.0000000
LPS+ATP:APOE4-ATP:APOE3 1673.0500000 1169.09477 2177.00523 0.0000000
LPS+NIG:APOE4-ATP:APOE3 1705.7250000 1201.76977 2209.68023 0.0000000
NIG:APOE4-ATP:APOE3 1.5000000 -502.45523 505.45523 1.0000000
Control:C57-ATP:APOE3 -1.4075000 -505.36273 502.54773 1.0000000
ATP:C57-ATP:APOE3 10.2683333 -534.06553 554.60219 1.0000000
LPS:C57-ATP:APOE3 2.7950000 -501.16023 506.75023 1.0000000
LPS+ATP:C57-ATP:APOE3 101.4783333 -442.85553 645.81219 1.0000000
LPS+NIG:C57-ATP:APOE3 1840.3450000 1296.01114 2384.67886 0.0000000
NIG:C57-ATP:APOE3 19.3450000 -524.98886 563.67886 1.0000000
Control:NULL-ATP:APOE3 7.4225000 -496.53273 511.37773 1.0000000
ATP:NULL-ATP:APOE3 9.4750000 -534.85886 553.80886 1.0000000
LPS:NULL-ATP:APOE3 8.8850000 -535.44886 553.21886 1.0000000
LPS+ATP:NULL-ATP:APOE3 474.2475000 -29.70773 978.20273 0.0906279
LPS+NIG:NULL-ATP:APOE3 1157.6450000 653.68977 1661.60023 0.0000000
NIG:NULL-ATP:APOE3 8.7483333 -535.58553 553.08219 1.0000000
LPS+ATP:APOE3-LPS:APOE3 1079.1666667 497.24929 1661.08404 0.0000005
LPS+NIG:APOE3-LPS:APOE3 1150.5250000 606.19114 1694.85886 0.0000000
NIG:APOE3-LPS:APOE3 0.9000000 -543.43386 545.23386 1.0000000
Control:APOE4-LPS:APOE3 -1.8000000 -546.13386 542.53386 1.0000000
ATP:APOE4-LPS:APOE3 -0.7000000 -582.61737 581.21737 1.0000000
LPS:APOE4-LPS:APOE3 8.9000000 -573.01737 590.81737 1.0000000
LPS+ATP:APOE4-LPS:APOE3 1671.0250000 1126.69114 2215.35886 0.0000000
LPS+NIG:APOE4-LPS:APOE3 1703.7000000 1159.36614 2248.03386 0.0000000
NIG:APOE4-LPS:APOE3 -0.5250000 -544.85886 543.80886 1.0000000
Control:C57-LPS:APOE3 -3.4325000 -547.76636 540.90136 1.0000000
ATP:C57-LPS:APOE3 8.2433333 -573.67404 590.16071 1.0000000
LPS:C57-LPS:APOE3 0.7700000 -543.56386 545.10386 1.0000000
LPS+ATP:C57-LPS:APOE3 99.4533333 -482.46404 681.37071 1.0000000
LPS+NIG:C57-LPS:APOE3 1838.3200000 1256.40263 2420.23737 0.0000000
NIG:C57-LPS:APOE3 17.3200000 -564.59737 599.23737 1.0000000
Control:NULL-LPS:APOE3 5.3975000 -538.93636 549.73136 1.0000000
ATP:NULL-LPS:APOE3 7.4500000 -574.46737 589.36737 1.0000000
LPS:NULL-LPS:APOE3 6.8600000 -575.05737 588.77737 1.0000000
LPS+ATP:NULL-LPS:APOE3 472.2225000 -72.11136 1016.55636 0.1767468
LPS+NIG:NULL-LPS:APOE3 1155.6200000 611.28614 1699.95386 0.0000000
NIG:NULL-LPS:APOE3 6.7233333 -575.19404 588.64071 1.0000000
LPS+NIG:APOE3-LPS+ATP:APOE3 71.3583333 -472.97553 615.69219 1.0000000
NIG:APOE3-LPS+ATP:APOE3 -1078.2666667 -1622.60053 -533.93281 0.0000001
Control:APOE4-LPS+ATP:APOE3 -1080.9666667 -1625.30053 -536.63281 0.0000001
ATP:APOE4-LPS+ATP:APOE3 -1079.8666667 -1661.78404 -497.94929 0.0000005
LPS:APOE4-LPS+ATP:APOE3 -1070.2666667 -1652.18404 -488.34929 0.0000006
LPS+ATP:APOE4-LPS+ATP:APOE3 591.8583333 47.52447 1136.19219 0.0191032
LPS+NIG:APOE4-LPS+ATP:APOE3 624.5333333 80.19947 1168.86719 0.0093999
NIG:APOE4-LPS+ATP:APOE3 -1079.6916667 -1624.02553 -535.35781 0.0000001
Control:C57-LPS+ATP:APOE3 -1082.5991667 -1626.93303 -538.26531 0.0000001
ATP:C57-LPS+ATP:APOE3 -1070.9233333 -1652.84071 -489.00596 0.0000006
LPS:C57-LPS+ATP:APOE3 -1078.3966667 -1622.73053 -534.06281 0.0000001
LPS+ATP:C57-LPS+ATP:APOE3 -979.7133333 -1561.63071 -397.79596 0.0000060
LPS+NIG:C57-LPS+ATP:APOE3 759.1533333 177.23596 1341.07071 0.0012706
NIG:C57-LPS+ATP:APOE3 -1061.8466667 -1643.76404 -479.92929 0.0000007
Control:NULL-LPS+ATP:APOE3 -1073.7691667 -1618.10303 -529.43531 0.0000001
ATP:NULL-LPS+ATP:APOE3 -1071.7166667 -1653.63404 -489.79929 0.0000006
LPS:NULL-LPS+ATP:APOE3 -1072.3066667 -1654.22404 -490.38929 0.0000006
LPS+ATP:NULL-LPS+ATP:APOE3 -606.9441667 -1151.27803 -62.61031 0.0138297
LPS+NIG:NULL-LPS+ATP:APOE3 76.4533333 -467.88053 620.78719 1.0000000
NIG:NULL-LPS+ATP:APOE3 -1072.4433333 -1654.36071 -490.52596 0.0000006
NIG:APOE3-LPS+NIG:APOE3 -1149.6250000 -1653.58023 -645.66977 0.0000000
Control:APOE4-LPS+NIG:APOE3 -1152.3250000 -1656.28023 -648.36977 0.0000000
ATP:APOE4-LPS+NIG:APOE3 -1151.2250000 -1695.55886 -606.89114 0.0000000
LPS:APOE4-LPS+NIG:APOE3 -1141.6250000 -1685.95886 -597.29114 0.0000000
LPS+ATP:APOE4-LPS+NIG:APOE3 520.5000000 16.54477 1024.45523 0.0351906
LPS+NIG:APOE4-LPS+NIG:APOE3 553.1750000 49.21977 1057.13023 0.0169458
NIG:APOE4-LPS+NIG:APOE3 -1151.0500000 -1655.00523 -647.09477 0.0000000
Control:C57-LPS+NIG:APOE3 -1153.9575000 -1657.91273 -650.00227 0.0000000
ATP:C57-LPS+NIG:APOE3 -1142.2816667 -1686.61553 -597.94781 0.0000000
LPS:C57-LPS+NIG:APOE3 -1149.7550000 -1653.71023 -645.79977 0.0000000
LPS+ATP:C57-LPS+NIG:APOE3 -1051.0716667 -1595.40553 -506.73781 0.0000002
LPS+NIG:C57-LPS+NIG:APOE3 687.7950000 143.46114 1232.12886 0.0021788
NIG:C57-LPS+NIG:APOE3 -1133.2050000 -1677.53886 -588.87114 0.0000000
Control:NULL-LPS+NIG:APOE3 -1145.1275000 -1649.08273 -641.17227 0.0000000
ATP:NULL-LPS+NIG:APOE3 -1143.0750000 -1687.40886 -598.74114 0.0000000
LPS:NULL-LPS+NIG:APOE3 -1143.6650000 -1687.99886 -599.33114 0.0000000
LPS+ATP:NULL-LPS+NIG:APOE3 -678.3025000 -1182.25773 -174.34727 0.0007297
LPS+NIG:NULL-LPS+NIG:APOE3 5.0950000 -498.86023 509.05023 1.0000000
NIG:NULL-LPS+NIG:APOE3 -1143.8016667 -1688.13553 -599.46781 0.0000000
Control:APOE4-NIG:APOE3 -2.7000000 -506.65523 501.25523 1.0000000
ATP:APOE4-NIG:APOE3 -1.6000000 -545.93386 542.73386 1.0000000
LPS:APOE4-NIG:APOE3 8.0000000 -536.33386 552.33386 1.0000000
LPS+ATP:APOE4-NIG:APOE3 1670.1250000 1166.16977 2174.08023 0.0000000
LPS+NIG:APOE4-NIG:APOE3 1702.8000000 1198.84477 2206.75523 0.0000000
NIG:APOE4-NIG:APOE3 -1.4250000 -505.38023 502.53023 1.0000000
Control:C57-NIG:APOE3 -4.3325000 -508.28773 499.62273 1.0000000
ATP:C57-NIG:APOE3 7.3433333 -536.99053 551.67719 1.0000000
LPS:C57-NIG:APOE3 -0.1300000 -504.08523 503.82523 1.0000000
LPS+ATP:C57-NIG:APOE3 98.5533333 -445.78053 642.88719 1.0000000
LPS+NIG:C57-NIG:APOE3 1837.4200000 1293.08614 2381.75386 0.0000000
NIG:C57-NIG:APOE3 16.4200000 -527.91386 560.75386 1.0000000
Control:NULL-NIG:APOE3 4.4975000 -499.45773 508.45273 1.0000000
ATP:NULL-NIG:APOE3 6.5500000 -537.78386 550.88386 1.0000000
LPS:NULL-NIG:APOE3 5.9600000 -538.37386 550.29386 1.0000000
LPS+ATP:NULL-NIG:APOE3 471.3225000 -32.63273 975.27773 0.0958343
LPS+NIG:NULL-NIG:APOE3 1154.7200000 650.76477 1658.67523 0.0000000
NIG:NULL-NIG:APOE3 5.8233333 -538.51053 550.15719 1.0000000
ATP:APOE4-Control:APOE4 1.1000000 -543.23386 545.43386 1.0000000
LPS:APOE4-Control:APOE4 10.7000000 -533.63386 555.03386 1.0000000
LPS+ATP:APOE4-Control:APOE4 1672.8250000 1168.86977 2176.78023 0.0000000
LPS+NIG:APOE4-Control:APOE4 1705.5000000 1201.54477 2209.45523 0.0000000
NIG:APOE4-Control:APOE4 1.2750000 -502.68023 505.23023 1.0000000
Control:C57-Control:APOE4 -1.6325000 -505.58773 502.32273 1.0000000
ATP:C57-Control:APOE4 10.0433333 -534.29053 554.37719 1.0000000
LPS:C57-Control:APOE4 2.5700000 -501.38523 506.52523 1.0000000
LPS+ATP:C57-Control:APOE4 101.2533333 -443.08053 645.58719 1.0000000
LPS+NIG:C57-Control:APOE4 1840.1200000 1295.78614 2384.45386 0.0000000
NIG:C57-Control:APOE4 19.1200000 -525.21386 563.45386 1.0000000
Control:NULL-Control:APOE4 7.1975000 -496.75773 511.15273 1.0000000
ATP:NULL-Control:APOE4 9.2500000 -535.08386 553.58386 1.0000000
LPS:NULL-Control:APOE4 8.6600000 -535.67386 552.99386 1.0000000
LPS+ATP:NULL-Control:APOE4 474.0225000 -29.93273 977.97773 0.0910198
LPS+NIG:NULL-Control:APOE4 1157.4200000 653.46477 1661.37523 0.0000000
NIG:NULL-Control:APOE4 8.5233333 -535.81053 552.85719 1.0000000
LPS:APOE4-ATP:APOE4 9.6000000 -572.31737 591.51737 1.0000000
LPS+ATP:APOE4-ATP:APOE4 1671.7250000 1127.39114 2216.05886 0.0000000
LPS+NIG:APOE4-ATP:APOE4 1704.4000000 1160.06614 2248.73386 0.0000000
NIG:APOE4-ATP:APOE4 0.1750000 -544.15886 544.50886 1.0000000
Control:C57-ATP:APOE4 -2.7325000 -547.06636 541.60136 1.0000000
ATP:C57-ATP:APOE4 8.9433333 -572.97404 590.86071 1.0000000
LPS:C57-ATP:APOE4 1.4700000 -542.86386 545.80386 1.0000000
LPS+ATP:C57-ATP:APOE4 100.1533333 -481.76404 682.07071 1.0000000
LPS+NIG:C57-ATP:APOE4 1839.0200000 1257.10263 2420.93737 0.0000000
NIG:C57-ATP:APOE4 18.0200000 -563.89737 599.93737 1.0000000
Control:NULL-ATP:APOE4 6.0975000 -538.23636 550.43136 1.0000000
ATP:NULL-ATP:APOE4 8.1500000 -573.76737 590.06737 1.0000000
LPS:NULL-ATP:APOE4 7.5600000 -574.35737 589.47737 1.0000000
LPS+ATP:NULL-ATP:APOE4 472.9225000 -71.41136 1017.25636 0.1748300
LPS+NIG:NULL-ATP:APOE4 1156.3200000 611.98614 1700.65386 0.0000000
NIG:NULL-ATP:APOE4 7.4233333 -574.49404 589.34071 1.0000000
LPS+ATP:APOE4-LPS:APOE4 1662.1250000 1117.79114 2206.45886 0.0000000
LPS+NIG:APOE4-LPS:APOE4 1694.8000000 1150.46614 2239.13386 0.0000000
NIG:APOE4-LPS:APOE4 -9.4250000 -553.75886 534.90886 1.0000000
Control:C57-LPS:APOE4 -12.3325000 -556.66636 532.00136 1.0000000
ATP:C57-LPS:APOE4 -0.6566667 -582.57404 581.26071 1.0000000
LPS:C57-LPS:APOE4 -8.1300000 -552.46386 536.20386 1.0000000
LPS+ATP:C57-LPS:APOE4 90.5533333 -491.36404 672.47071 1.0000000
LPS+NIG:C57-LPS:APOE4 1829.4200000 1247.50263 2411.33737 0.0000000
NIG:C57-LPS:APOE4 8.4200000 -573.49737 590.33737 1.0000000
Control:NULL-LPS:APOE4 -3.5025000 -547.83636 540.83136 1.0000000
ATP:NULL-LPS:APOE4 -1.4500000 -583.36737 580.46737 1.0000000
LPS:NULL-LPS:APOE4 -2.0400000 -583.95737 579.87737 1.0000000
LPS+ATP:NULL-LPS:APOE4 463.3225000 -81.01136 1007.65636 0.2025063
LPS+NIG:NULL-LPS:APOE4 1146.7200000 602.38614 1691.05386 0.0000000
NIG:NULL-LPS:APOE4 -2.1766667 -584.09404 579.74071 1.0000000
LPS+NIG:APOE4-LPS+ATP:APOE4 32.6750000 -471.28023 536.63023 1.0000000
NIG:APOE4-LPS+ATP:APOE4 -1671.5500000 -2175.50523 -1167.59477 0.0000000
Control:C57-LPS+ATP:APOE4 -1674.4575000 -2178.41273 -1170.50227 0.0000000
ATP:C57-LPS+ATP:APOE4 -1662.7816667 -2207.11553 -1118.44781 0.0000000
LPS:C57-LPS+ATP:APOE4 -1670.2550000 -2174.21023 -1166.29977 0.0000000
LPS+ATP:C57-LPS+ATP:APOE4 -1571.5716667 -2115.90553 -1027.23781 0.0000000
LPS+NIG:C57-LPS+ATP:APOE4 167.2950000 -377.03886 711.62886 0.9999403
NIG:C57-LPS+ATP:APOE4 -1653.7050000 -2198.03886 -1109.37114 0.0000000
Control:NULL-LPS+ATP:APOE4 -1665.6275000 -2169.58273 -1161.67227 0.0000000
ATP:NULL-LPS+ATP:APOE4 -1663.5750000 -2207.90886 -1119.24114 0.0000000
LPS:NULL-LPS+ATP:APOE4 -1664.1650000 -2208.49886 -1119.83114 0.0000000
LPS+ATP:NULL-LPS+ATP:APOE4 -1198.8025000 -1702.75773 -694.84727 0.0000000
LPS+NIG:NULL-LPS+ATP:APOE4 -515.4050000 -1019.36023 -11.44977 0.0392666
NIG:NULL-LPS+ATP:APOE4 -1664.3016667 -2208.63553 -1119.96781 0.0000000
NIG:APOE4-LPS+NIG:APOE4 -1704.2250000 -2208.18023 -1200.26977 0.0000000
Control:C57-LPS+NIG:APOE4 -1707.1325000 -2211.08773 -1203.17727 0.0000000
ATP:C57-LPS+NIG:APOE4 -1695.4566667 -2239.79053 -1151.12281 0.0000000
LPS:C57-LPS+NIG:APOE4 -1702.9300000 -2206.88523 -1198.97477 0.0000000
LPS+ATP:C57-LPS+NIG:APOE4 -1604.2466667 -2148.58053 -1059.91281 0.0000000
LPS+NIG:C57-LPS+NIG:APOE4 134.6200000 -409.71386 678.95386 0.9999987
NIG:C57-LPS+NIG:APOE4 -1686.3800000 -2230.71386 -1142.04614 0.0000000
Control:NULL-LPS+NIG:APOE4 -1698.3025000 -2202.25773 -1194.34727 0.0000000
ATP:NULL-LPS+NIG:APOE4 -1696.2500000 -2240.58386 -1151.91614 0.0000000
LPS:NULL-LPS+NIG:APOE4 -1696.8400000 -2241.17386 -1152.50614 0.0000000
LPS+ATP:NULL-LPS+NIG:APOE4 -1231.4775000 -1735.43273 -727.52227 0.0000000
LPS+NIG:NULL-LPS+NIG:APOE4 -548.0800000 -1052.03523 -44.12477 0.0190484
NIG:NULL-LPS+NIG:APOE4 -1696.9766667 -2241.31053 -1152.64281 0.0000000
Control:C57-NIG:APOE4 -2.9075000 -506.86273 501.04773 1.0000000
ATP:C57-NIG:APOE4 8.7683333 -535.56553 553.10219 1.0000000
LPS:C57-NIG:APOE4 1.2950000 -502.66023 505.25023 1.0000000
LPS+ATP:C57-NIG:APOE4 99.9783333 -444.35553 644.31219 1.0000000
LPS+NIG:C57-NIG:APOE4 1838.8450000 1294.51114 2383.17886 0.0000000
NIG:C57-NIG:APOE4 17.8450000 -526.48886 562.17886 1.0000000
Control:NULL-NIG:APOE4 5.9225000 -498.03273 509.87773 1.0000000
ATP:NULL-NIG:APOE4 7.9750000 -536.35886 552.30886 1.0000000
LPS:NULL-NIG:APOE4 7.3850000 -536.94886 551.71886 1.0000000
LPS+ATP:NULL-NIG:APOE4 472.7475000 -31.20773 976.70273 0.0932674
LPS+NIG:NULL-NIG:APOE4 1156.1450000 652.18977 1660.10023 0.0000000
NIG:NULL-NIG:APOE4 7.2483333 -537.08553 551.58219 1.0000000
ATP:C57-Control:C57 11.6758333 -532.65803 556.00969 1.0000000
LPS:C57-Control:C57 4.2025000 -499.75273 508.15773 1.0000000
LPS+ATP:C57-Control:C57 102.8858333 -441.44803 647.21969 1.0000000
LPS+NIG:C57-Control:C57 1841.7525000 1297.41864 2386.08636 0.0000000
NIG:C57-Control:C57 20.7525000 -523.58136 565.08636 1.0000000
Control:NULL-Control:C57 8.8300000 -495.12523 512.78523 1.0000000
ATP:NULL-Control:C57 10.8825000 -533.45136 555.21636 1.0000000
LPS:NULL-Control:C57 10.2925000 -534.04136 554.62636 1.0000000
LPS+ATP:NULL-Control:C57 475.6550000 -28.30023 979.61023 0.0882085
LPS+NIG:NULL-Control:C57 1159.0525000 655.09727 1663.00773 0.0000000
NIG:NULL-Control:C57 10.1558333 -534.17803 554.48969 1.0000000
LPS:C57-ATP:C57 -7.4733333 -551.80719 536.86053 1.0000000
LPS+ATP:C57-ATP:C57 91.2100000 -490.70737 673.12737 1.0000000
LPS+NIG:C57-ATP:C57 1830.0766667 1248.15929 2411.99404 0.0000000
NIG:C57-ATP:C57 9.0766667 -572.84071 590.99404 1.0000000
Control:NULL-ATP:C57 -2.8458333 -547.17969 541.48803 1.0000000
ATP:NULL-ATP:C57 -0.7933333 -582.71071 581.12404 1.0000000
LPS:NULL-ATP:C57 -1.3833333 -583.30071 580.53404 1.0000000
LPS+ATP:NULL-ATP:C57 463.9791667 -80.35469 1008.31303 0.2005168
LPS+NIG:NULL-ATP:C57 1147.3766667 603.04281 1691.71053 0.0000000
NIG:NULL-ATP:C57 -1.5200000 -583.43737 580.39737 1.0000000
LPS+ATP:C57-LPS:C57 98.6833333 -445.65053 643.01719 1.0000000
LPS+NIG:C57-LPS:C57 1837.5500000 1293.21614 2381.88386 0.0000000
NIG:C57-LPS:C57 16.5500000 -527.78386 560.88386 1.0000000
Control:NULL-LPS:C57 4.6275000 -499.32773 508.58273 1.0000000
ATP:NULL-LPS:C57 6.6800000 -537.65386 551.01386 1.0000000
LPS:NULL-LPS:C57 6.0900000 -538.24386 550.42386 1.0000000
LPS+ATP:NULL-LPS:C57 471.4525000 -32.50273 975.40773 0.0955977
LPS+NIG:NULL-LPS:C57 1154.8500000 650.89477 1658.80523 0.0000000
NIG:NULL-LPS:C57 5.9533333 -538.38053 550.28719 1.0000000
LPS+NIG:C57-LPS+ATP:C57 1738.8666667 1156.94929 2320.78404 0.0000000
NIG:C57-LPS+ATP:C57 -82.1333333 -664.05071 499.78404 1.0000000
Control:NULL-LPS+ATP:C57 -94.0558333 -638.38969 450.27803 1.0000000
ATP:NULL-LPS+ATP:C57 -92.0033333 -673.92071 489.91404 1.0000000
LPS:NULL-LPS+ATP:C57 -92.5933333 -674.51071 489.32404 1.0000000
LPS+ATP:NULL-LPS+ATP:C57 372.7691667 -171.56469 917.10303 0.5946677
LPS+NIG:NULL-LPS+ATP:C57 1056.1666667 511.83281 1600.50053 0.0000001
NIG:NULL-LPS+ATP:C57 -92.7300000 -674.64737 489.18737 1.0000000
NIG:C57-LPS+NIG:C57 -1821.0000000 -2402.91737 -1239.08263 0.0000000
Control:NULL-LPS+NIG:C57 -1832.9225000 -2377.25636 -1288.58864 0.0000000
ATP:NULL-LPS+NIG:C57 -1830.8700000 -2412.78737 -1248.95263 0.0000000
LPS:NULL-LPS+NIG:C57 -1831.4600000 -2413.37737 -1249.54263 0.0000000
LPS+ATP:NULL-LPS+NIG:C57 -1366.0975000 -1910.43136 -821.76364 0.0000000
LPS+NIG:NULL-LPS+NIG:C57 -682.7000000 -1227.03386 -138.36614 0.0024603
NIG:NULL-LPS+NIG:C57 -1831.5966667 -2413.51404 -1249.67929 0.0000000
Control:NULL-NIG:C57 -11.9225000 -556.25636 532.41136 1.0000000
ATP:NULL-NIG:C57 -9.8700000 -591.78737 572.04737 1.0000000
LPS:NULL-NIG:C57 -10.4600000 -592.37737 571.45737 1.0000000
LPS+ATP:NULL-NIG:C57 454.9025000 -89.43136 999.23636 0.2292868
LPS+NIG:NULL-NIG:C57 1138.3000000 593.96614 1682.63386 0.0000000
NIG:NULL-NIG:C57 -10.5966667 -592.51404 571.32071 1.0000000
ATP:NULL-Control:NULL 2.0525000 -542.28136 546.38636 1.0000000
LPS:NULL-Control:NULL 1.4625000 -542.87136 545.79636 1.0000000
LPS+ATP:NULL-Control:NULL 466.8250000 -37.13023 970.78023 0.1043248
LPS+NIG:NULL-Control:NULL 1150.2225000 646.26727 1654.17773 0.0000000
NIG:NULL-Control:NULL 1.3258333 -543.00803 545.65969 1.0000000
LPS:NULL-ATP:NULL -0.5900000 -582.50737 581.32737 1.0000000
LPS+ATP:NULL-ATP:NULL 464.7725000 -79.56136 1009.10636 0.1981323
LPS+NIG:NULL-ATP:NULL 1148.1700000 603.83614 1692.50386 0.0000000
NIG:NULL-ATP:NULL -0.7266667 -582.64404 581.19071 1.0000000
LPS+ATP:NULL-LPS:NULL 465.3625000 -78.97136 1009.69636 0.1963724
LPS+NIG:NULL-LPS:NULL 1148.7600000 604.42614 1693.09386 0.0000000
NIG:NULL-LPS:NULL -0.1366667 -582.05404 581.78071 1.0000000
LPS+NIG:NULL-LPS+ATP:NULL 683.3975000 179.44227 1187.35273 0.0006363
NIG:NULL-LPS+ATP:NULL -465.4991667 -1009.83303 78.83469 0.1959664
NIG:NULL-LPS+NIG:NULL -1148.8966667 -1693.23053 -604.56281 0.0000000