Code
library(readxl)
library(car)
library(multcomp)
library(ggplot2)
library(writexl)NB Project: Active Caspase
Code
# Load the data
NB_data <- read_excel("Fold Change Calculations for Final Data_tidy.xlsx", sheet = 2)
data3 <- NB_data
# Display the first few rows of the imported data
head(data3)# A tibble: 6 × 4
Sample Gene Treatment active_caspase_1
<dbl> <chr> <chr> <dbl>
1 1 C57 Control 9.39
2 2 C57 LPS 15.6
3 3 C57 ATP 8.79
4 4 C57 LPS+ATP 72.0
5 5 C57 NIG 10.5
6 6 C57 LPS+NIG 238. Code
# Convert relevant columns to factors (Assuming 'Treatment' and 'Gene' are columns)
data3$Treatment <- factor(data3$Treatment, levels = c(
"Control", "ATP", "LPS", "LPS+ATP", "LPS+NIG", "NIG"))
data3$Gene <- as.factor(data3$Gene)
head(data3)# A tibble: 6 × 4
Sample Gene Treatment active_caspase_1
<dbl> <fct> <fct> <dbl>
1 1 C57 Control 9.39
2 2 C57 LPS 15.6
3 3 C57 ATP 8.79
4 4 C57 LPS+ATP 72.0
5 5 C57 NIG 10.5
6 6 C57 LPS+NIG 238. Code
# Perform a two-way ANOVA (Assuming 'JESS_Active_Caspase_1' is the dependent variable)
anova_result <- aov(active_caspase_1 ~ Treatment * Gene, data = data3)
# Display the summary of ANOVA results
summary(anova_result) Df Sum Sq Mean Sq F value Pr(>F)
Treatment 5 23544867 4708973 13.835 1.73e-08 ***
Gene 3 6066123 2022041 5.941 0.001512 **
Treatment:Gene 15 20211908 1347461 3.959 0.000119 ***
Residuals 50 17018747 340375
---
Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
4 observations deleted due to missingnessCode
# Perform Tukey's HSD post-hoc test for multiple comparisons
tukey_result <- TukeyHSD(anova_result)
print(tukey_result) Tukey multiple comparisons of means
95% family-wise confidence level
Fit: aov(formula = active_caspase_1 ~ Treatment * Gene, data = data3)
$Treatment
diff lwr upr p adj
ATP-Control 4.6516667 -701.0858 710.3891 1.0000000
LPS-Control 0.3789103 -691.6536 692.4114 1.0000000
LPS+ATP-Control 1013.9966667 308.2592 1719.7341 0.0012174
LPS+NIG-Control 1365.0758333 659.3384 2070.8133 0.0000082
NIG-Control 9.4366026 -682.5959 701.4691 1.0000000
LPS-ATP -4.2727564 -696.3053 687.7598 1.0000000
LPS+ATP-ATP 1009.3450000 303.6075 1715.0825 0.0012954
LPS+NIG-ATP 1360.4241667 654.6867 2066.1616 0.0000088
NIG-ATP 4.7849359 -687.2476 696.8175 1.0000000
LPS+ATP-LPS 1013.6177564 321.5852 1705.6503 0.0009342
LPS+NIG-LPS 1364.6969231 672.6644 2056.7295 0.0000055
NIG-LPS 9.0576923 -668.9929 687.1083 1.0000000
LPS+NIG-LPS+ATP 351.0791667 -354.6583 1056.8166 0.6819832
NIG-LPS+ATP -1004.5600641 -1696.5926 -312.5275 0.0010580
NIG-LPS+NIG -1355.6392308 -2047.6718 -663.6067 0.0000064
$Gene
diff lwr upr p adj
APOE4-APOE3 -139.97775 -638.0044 358.048930 0.8775269
C57-APOE3 -666.14719 -1164.1739 -168.120514 0.0045107
NULL-APOE3 -580.94242 -1086.7949 -75.089925 0.0184570
C57-APOE4 -526.16944 -1042.9962 -9.342738 0.0445437
NULL-APOE4 -440.96467 -965.3367 83.407351 0.1278814
NULL-C57 85.20477 -439.1672 609.576796 0.9727236
$`Treatment:Gene`
diff lwr upr p adj
ATP:APOE3-Control:APOE3 21.1691667 -1692.4369 1734.775256 1.0000000
LPS:APOE3-Control:APOE3 11.7650000 -1574.7260 1598.255961 1.0000000
LPS+ATP:APOE3-Control:APOE3 1224.1225000 -489.4836 2937.728590 0.4970049
LPS+NIG:APOE3-Control:APOE3 3471.1858333 1757.5797 5184.791923 0.0000001
NIG:APOE3-Control:APOE3 10.9675000 -1575.5235 1597.458461 1.0000000
Control:APOE4-Control:APOE3 8.2391667 -1705.3669 1721.845256 1.0000000
ATP:APOE4-Control:APOE3 17.9458333 -1695.6603 1731.551923 1.0000000
LPS:APOE4-Control:APOE3 5.4191667 -1708.1869 1719.025256 1.0000000
LPS+ATP:APOE4-Control:APOE3 2006.8358333 293.2297 3720.441923 0.0075513
LPS+NIG:APOE4-Control:APOE3 1495.8958333 -217.7103 3209.501923 0.1641340
NIG:APOE4-Control:APOE3 33.6025000 -1680.0036 1747.208590 1.0000000
Control:C57-Control:APOE3 8.9358333 -1704.6703 1722.541923 1.0000000
ATP:C57-Control:APOE3 7.2791667 -1706.3269 1720.885256 1.0000000
LPS:C57-Control:APOE3 5.2691667 -1708.3369 1718.875256 1.0000000
LPS+ATP:C57-Control:APOE3 168.1458333 -1545.4603 1881.751923 1.0000000
LPS+NIG:C57-Control:APOE3 221.6325000 -1491.9736 1935.238590 1.0000000
NIG:C57-Control:APOE3 -0.3408333 -1713.9469 1713.265256 1.0000000
Control:NULL-Control:APOE3 11.2375000 -1931.8092 1954.284168 1.0000000
ATP:NULL-Control:APOE3 -3.1208333 -1716.7269 1710.485256 1.0000000
LPS:NULL-Control:APOE3 1.9891667 -1711.6169 1715.595256 1.0000000
LPS+ATP:NULL-Control:APOE3 681.5491667 -1032.0569 2395.155256 0.9963404
LPS+NIG:NULL-Control:APOE3 296.2558333 -1417.3503 2009.861923 1.0000000
NIG:NULL-Control:APOE3 19.7291667 -1693.8769 1733.335256 1.0000000
LPS:APOE3-ATP:APOE3 -9.4041667 -1723.0103 1704.201923 1.0000000
LPS+ATP:APOE3-ATP:APOE3 1202.9533333 -628.9686 3034.875300 0.6532429
LPS+NIG:APOE3-ATP:APOE3 3450.0166667 1618.0947 5281.938633 0.0000006
NIG:APOE3-ATP:APOE3 -10.2016667 -1723.8078 1703.404423 1.0000000
Control:APOE4-ATP:APOE3 -12.9300000 -1844.8520 1818.991967 1.0000000
ATP:APOE4-ATP:APOE3 -3.2233333 -1835.1453 1828.698633 1.0000000
LPS:APOE4-ATP:APOE3 -15.7500000 -1847.6720 1816.171967 1.0000000
LPS+ATP:APOE4-ATP:APOE3 1985.6666667 153.7447 3817.588633 0.0204698
LPS+NIG:APOE4-ATP:APOE3 1474.7266667 -357.1953 3306.648633 0.2798037
NIG:APOE4-ATP:APOE3 12.4333333 -1819.4886 1844.355300 1.0000000
Control:C57-ATP:APOE3 -12.2333333 -1844.1553 1819.688633 1.0000000
ATP:C57-ATP:APOE3 -13.8900000 -1845.8120 1818.031967 1.0000000
LPS:C57-ATP:APOE3 -15.9000000 -1847.8220 1816.021967 1.0000000
LPS+ATP:C57-ATP:APOE3 146.9766667 -1684.9453 1978.898633 1.0000000
LPS+NIG:C57-ATP:APOE3 200.4633333 -1631.4586 2032.385300 1.0000000
NIG:C57-ATP:APOE3 -21.5100000 -1853.4320 1810.411967 1.0000000
Control:NULL-ATP:APOE3 -9.9316667 -2058.0827 2038.219357 1.0000000
ATP:NULL-ATP:APOE3 -24.2900000 -1856.2120 1807.631967 1.0000000
LPS:NULL-ATP:APOE3 -19.1800000 -1851.1020 1812.741967 1.0000000
LPS+ATP:NULL-ATP:APOE3 660.3800000 -1171.5420 2492.301967 0.9990436
LPS+NIG:NULL-ATP:APOE3 275.0866667 -1556.8353 2107.008633 1.0000000
NIG:NULL-ATP:APOE3 -1.4400000 -1833.3620 1830.481967 1.0000000
LPS+ATP:APOE3-LPS:APOE3 1212.3575000 -501.2486 2925.963590 0.5154508
LPS+NIG:APOE3-LPS:APOE3 3459.4208333 1745.8147 5173.026923 0.0000001
NIG:APOE3-LPS:APOE3 -0.7975000 -1587.2885 1585.693461 1.0000000
Control:APOE4-LPS:APOE3 -3.5258333 -1717.1319 1710.080256 1.0000000
ATP:APOE4-LPS:APOE3 6.1808333 -1707.4253 1719.786923 1.0000000
LPS:APOE4-LPS:APOE3 -6.3458333 -1719.9519 1707.260256 1.0000000
LPS+ATP:APOE4-LPS:APOE3 1995.0708333 281.4647 3708.676923 0.0081861
LPS+NIG:APOE4-LPS:APOE3 1484.1308333 -229.4753 3197.736923 0.1738478
NIG:APOE4-LPS:APOE3 21.8375000 -1691.7686 1735.443590 1.0000000
Control:C57-LPS:APOE3 -2.8291667 -1716.4353 1710.776923 1.0000000
ATP:C57-LPS:APOE3 -4.4858333 -1718.0919 1709.120256 1.0000000
LPS:C57-LPS:APOE3 -6.4958333 -1720.1019 1707.110256 1.0000000
LPS+ATP:C57-LPS:APOE3 156.3808333 -1557.2253 1869.986923 1.0000000
LPS+NIG:C57-LPS:APOE3 209.8675000 -1503.7386 1923.473590 1.0000000
NIG:C57-LPS:APOE3 -12.1058333 -1725.7119 1701.500256 1.0000000
Control:NULL-LPS:APOE3 -0.5275000 -1943.5742 1942.519168 1.0000000
ATP:NULL-LPS:APOE3 -14.8858333 -1728.4919 1698.720256 1.0000000
LPS:NULL-LPS:APOE3 -9.7758333 -1723.3819 1703.830256 1.0000000
LPS+ATP:NULL-LPS:APOE3 669.7841667 -1043.8219 2383.390256 0.9970865
LPS+NIG:NULL-LPS:APOE3 284.4908333 -1429.1153 1998.096923 1.0000000
NIG:NULL-LPS:APOE3 7.9641667 -1705.6419 1721.570256 1.0000000
LPS+NIG:APOE3-LPS+ATP:APOE3 2247.0633333 415.1414 4078.985300 0.0038853
NIG:APOE3-LPS+ATP:APOE3 -1213.1550000 -2926.7611 500.451090 0.5141960
Control:APOE4-LPS+ATP:APOE3 -1215.8833333 -3047.8053 616.038633 0.6343207
ATP:APOE4-LPS+ATP:APOE3 -1206.1766667 -3038.0986 625.745300 0.6485426
LPS:APOE4-LPS+ATP:APOE3 -1218.7033333 -3050.6253 613.218633 0.6301719
LPS+ATP:APOE4-LPS+ATP:APOE3 782.7133333 -1049.2086 2614.635300 0.9910698
LPS+NIG:APOE4-LPS+ATP:APOE3 271.7733333 -1560.1486 2103.695300 1.0000000
NIG:APOE4-LPS+ATP:APOE3 -1190.5200000 -3022.4420 641.401967 0.6712499
Control:C57-LPS+ATP:APOE3 -1215.1866667 -3047.1086 616.735300 0.6353445
ATP:C57-LPS+ATP:APOE3 -1216.8433333 -3048.7653 615.078633 0.6329091
LPS:C57-LPS+ATP:APOE3 -1218.8533333 -3050.7753 613.068633 0.6299511
LPS+ATP:C57-LPS+ATP:APOE3 -1055.9766667 -2887.8986 775.945300 0.8431230
LPS+NIG:C57-LPS+ATP:APOE3 -1002.4900000 -2834.4120 829.431967 0.8941393
NIG:C57-LPS+ATP:APOE3 -1224.4633333 -3056.3853 607.458633 0.6216777
Control:NULL-LPS+ATP:APOE3 -1212.8850000 -3261.0360 835.266024 0.8109412
ATP:NULL-LPS+ATP:APOE3 -1227.2433333 -3059.1653 604.678633 0.6175693
LPS:NULL-LPS+ATP:APOE3 -1222.1333333 -3054.0553 609.788633 0.6251168
LPS+ATP:NULL-LPS+ATP:APOE3 -542.5733333 -2374.4953 1289.348633 0.9999545
LPS+NIG:NULL-LPS+ATP:APOE3 -927.8666667 -2759.7886 904.055300 0.9456906
NIG:NULL-LPS+ATP:APOE3 -1204.3933333 -3036.3153 627.528633 0.6511446
NIG:APOE3-LPS+NIG:APOE3 -3460.2183333 -5173.8244 -1746.612244 0.0000001
Control:APOE4-LPS+NIG:APOE3 -3462.9466667 -5294.8686 -1631.024700 0.0000006
ATP:APOE4-LPS+NIG:APOE3 -3453.2400000 -5285.1620 -1621.318033 0.0000006
LPS:APOE4-LPS+NIG:APOE3 -3465.7666667 -5297.6886 -1633.844700 0.0000006
LPS+ATP:APOE4-LPS+NIG:APOE3 -1464.3500000 -3296.2720 367.571967 0.2914324
LPS+NIG:APOE4-LPS+NIG:APOE3 -1975.2900000 -3807.2120 -143.368033 0.0217903
NIG:APOE4-LPS+NIG:APOE3 -3437.5833333 -5269.5053 -1605.661367 0.0000007
Control:C57-LPS+NIG:APOE3 -3462.2500000 -5294.1720 -1630.328033 0.0000006
ATP:C57-LPS+NIG:APOE3 -3463.9066667 -5295.8286 -1631.984700 0.0000006
LPS:C57-LPS+NIG:APOE3 -3465.9166667 -5297.8386 -1633.994700 0.0000006
LPS+ATP:C57-LPS+NIG:APOE3 -3303.0400000 -5134.9620 -1471.118033 0.0000019
LPS+NIG:C57-LPS+NIG:APOE3 -3249.5533333 -5081.4753 -1417.631367 0.0000029
NIG:C57-LPS+NIG:APOE3 -3471.5266667 -5303.4486 -1639.604700 0.0000005
Control:NULL-LPS+NIG:APOE3 -3459.9483333 -5508.0994 -1411.797310 0.0000091
ATP:NULL-LPS+NIG:APOE3 -3474.3066667 -5306.2286 -1642.384700 0.0000005
LPS:NULL-LPS+NIG:APOE3 -3469.1966667 -5301.1186 -1637.274700 0.0000006
LPS+ATP:NULL-LPS+NIG:APOE3 -2789.6366667 -4621.5586 -957.714700 0.0000864
LPS+NIG:NULL-LPS+NIG:APOE3 -3174.9300000 -5006.8520 -1343.008033 0.0000050
NIG:NULL-LPS+NIG:APOE3 -3451.4566667 -5283.3786 -1619.534700 0.0000006
Control:APOE4-NIG:APOE3 -2.7283333 -1716.3344 1710.877756 1.0000000
ATP:APOE4-NIG:APOE3 6.9783333 -1706.6278 1720.584423 1.0000000
LPS:APOE4-NIG:APOE3 -5.5483333 -1719.1544 1708.057756 1.0000000
LPS+ATP:APOE4-NIG:APOE3 1995.8683333 282.2622 3709.474423 0.0081415
LPS+NIG:APOE4-NIG:APOE3 1484.9283333 -228.6778 3198.534423 0.1731758
NIG:APOE4-NIG:APOE3 22.6350000 -1690.9711 1736.241090 1.0000000
Control:C57-NIG:APOE3 -2.0316667 -1715.6378 1711.574423 1.0000000
ATP:C57-NIG:APOE3 -3.6883333 -1717.2944 1709.917756 1.0000000
LPS:C57-NIG:APOE3 -5.6983333 -1719.3044 1707.907756 1.0000000
LPS+ATP:C57-NIG:APOE3 157.1783333 -1556.4278 1870.784423 1.0000000
LPS+NIG:C57-NIG:APOE3 210.6650000 -1502.9411 1924.271090 1.0000000
NIG:C57-NIG:APOE3 -11.3083333 -1724.9144 1702.297756 1.0000000
Control:NULL-NIG:APOE3 0.2700000 -1942.7767 1943.316668 1.0000000
ATP:NULL-NIG:APOE3 -14.0883333 -1727.6944 1699.517756 1.0000000
LPS:NULL-NIG:APOE3 -8.9783333 -1722.5844 1704.627756 1.0000000
LPS+ATP:NULL-NIG:APOE3 670.5816667 -1043.0244 2384.187756 0.9970404
LPS+NIG:NULL-NIG:APOE3 285.2883333 -1428.3178 1998.894423 1.0000000
NIG:NULL-NIG:APOE3 8.7616667 -1704.8444 1722.367756 1.0000000
ATP:APOE4-Control:APOE4 9.7066667 -1822.2153 1841.628633 1.0000000
LPS:APOE4-Control:APOE4 -2.8200000 -1834.7420 1829.101967 1.0000000
LPS+ATP:APOE4-Control:APOE4 1998.5966667 166.6747 3830.518633 0.0189280
LPS+NIG:APOE4-Control:APOE4 1487.6566667 -344.2653 3319.578633 0.2657362
NIG:APOE4-Control:APOE4 25.3633333 -1806.5586 1857.285300 1.0000000
Control:C57-Control:APOE4 0.6966667 -1831.2253 1832.618633 1.0000000
ATP:C57-Control:APOE4 -0.9600000 -1832.8820 1830.961967 1.0000000
LPS:C57-Control:APOE4 -2.9700000 -1834.8920 1828.951967 1.0000000
LPS+ATP:C57-Control:APOE4 159.9066667 -1672.0153 1991.828633 1.0000000
LPS+NIG:C57-Control:APOE4 213.3933333 -1618.5286 2045.315300 1.0000000
NIG:C57-Control:APOE4 -8.5800000 -1840.5020 1823.341967 1.0000000
Control:NULL-Control:APOE4 2.9983333 -2045.1527 2051.149357 1.0000000
ATP:NULL-Control:APOE4 -11.3600000 -1843.2820 1820.561967 1.0000000
LPS:NULL-Control:APOE4 -6.2500000 -1838.1720 1825.671967 1.0000000
LPS+ATP:NULL-Control:APOE4 673.3100000 -1158.6120 2505.231967 0.9987410
LPS+NIG:NULL-Control:APOE4 288.0166667 -1543.9053 2119.938633 1.0000000
NIG:NULL-Control:APOE4 11.4900000 -1820.4320 1843.411967 1.0000000
LPS:APOE4-ATP:APOE4 -12.5266667 -1844.4486 1819.395300 1.0000000
LPS+ATP:APOE4-ATP:APOE4 1988.8900000 156.9680 3820.811967 0.0200750
LPS+NIG:APOE4-ATP:APOE4 1477.9500000 -353.9720 3309.871967 0.2762527
NIG:APOE4-ATP:APOE4 15.6566667 -1816.2653 1847.578633 1.0000000
Control:C57-ATP:APOE4 -9.0100000 -1840.9320 1822.911967 1.0000000
ATP:C57-ATP:APOE4 -10.6666667 -1842.5886 1821.255300 1.0000000
LPS:C57-ATP:APOE4 -12.6766667 -1844.5986 1819.245300 1.0000000
LPS+ATP:C57-ATP:APOE4 150.2000000 -1681.7220 1982.121967 1.0000000
LPS+NIG:C57-ATP:APOE4 203.6866667 -1628.2353 2035.608633 1.0000000
NIG:C57-ATP:APOE4 -18.2866667 -1850.2086 1813.635300 1.0000000
Control:NULL-ATP:APOE4 -6.7083333 -2054.8594 2041.442690 1.0000000
ATP:NULL-ATP:APOE4 -21.0666667 -1852.9886 1810.855300 1.0000000
LPS:NULL-ATP:APOE4 -15.9566667 -1847.8786 1815.965300 1.0000000
LPS+ATP:NULL-ATP:APOE4 663.6033333 -1168.3186 2495.525300 0.9989748
LPS+NIG:NULL-ATP:APOE4 278.3100000 -1553.6120 2110.231967 1.0000000
NIG:NULL-ATP:APOE4 1.7833333 -1830.1386 1833.705300 1.0000000
LPS+ATP:APOE4-LPS:APOE4 2001.4166667 169.4947 3833.338633 0.0186063
LPS+NIG:APOE4-LPS:APOE4 1490.4766667 -341.4453 3322.398633 0.2627309
NIG:APOE4-LPS:APOE4 28.1833333 -1803.7386 1860.105300 1.0000000
Control:C57-LPS:APOE4 3.5166667 -1828.4053 1835.438633 1.0000000
ATP:C57-LPS:APOE4 1.8600000 -1830.0620 1833.781967 1.0000000
LPS:C57-LPS:APOE4 -0.1500000 -1832.0720 1831.771967 1.0000000
LPS+ATP:C57-LPS:APOE4 162.7266667 -1669.1953 1994.648633 1.0000000
LPS+NIG:C57-LPS:APOE4 216.2133333 -1615.7086 2048.135300 1.0000000
NIG:C57-LPS:APOE4 -5.7600000 -1837.6820 1826.161967 1.0000000
Control:NULL-LPS:APOE4 5.8183333 -2042.3327 2053.969357 1.0000000
ATP:NULL-LPS:APOE4 -8.5400000 -1840.4620 1823.381967 1.0000000
LPS:NULL-LPS:APOE4 -3.4300000 -1835.3520 1828.491967 1.0000000
LPS+ATP:NULL-LPS:APOE4 676.1300000 -1155.7920 2508.051967 0.9986649
LPS+NIG:NULL-LPS:APOE4 290.8366667 -1541.0853 2122.758633 1.0000000
NIG:NULL-LPS:APOE4 14.3100000 -1817.6120 1846.231967 1.0000000
LPS+NIG:APOE4-LPS+ATP:APOE4 -510.9400000 -2342.8620 1320.981967 0.9999835
NIG:APOE4-LPS+ATP:APOE4 -1973.2333333 -3805.1553 -141.311367 0.0220612
Control:C57-LPS+ATP:APOE4 -1997.9000000 -3829.8220 -165.978033 0.0190082
ATP:C57-LPS+ATP:APOE4 -1999.5566667 -3831.4786 -167.634700 0.0188179
LPS:C57-LPS+ATP:APOE4 -2001.5666667 -3833.4886 -169.644700 0.0185893
LPS+ATP:C57-LPS+ATP:APOE4 -1838.6900000 -3670.6120 -6.768033 0.0481494
LPS+NIG:C57-LPS+ATP:APOE4 -1785.2033333 -3617.1253 46.718633 0.0645813
NIG:C57-LPS+ATP:APOE4 -2007.1766667 -3839.0986 -175.254700 0.0179649
Control:NULL-LPS+ATP:APOE4 -1995.5983333 -4043.7494 52.552690 0.0646809
ATP:NULL-LPS+ATP:APOE4 -2009.9566667 -3841.8786 -178.034700 0.0176628
LPS:NULL-LPS+ATP:APOE4 -2004.8466667 -3836.7686 -172.924700 0.0182219
LPS+ATP:NULL-LPS+ATP:APOE4 -1325.2866667 -3157.2086 506.635300 0.4728518
LPS+NIG:NULL-LPS+ATP:APOE4 -1710.5800000 -3542.5020 121.341967 0.0955453
NIG:NULL-LPS+ATP:APOE4 -1987.1066667 -3819.0286 -155.184700 0.0202926
NIG:APOE4-LPS+NIG:APOE4 -1462.2933333 -3294.2153 369.628633 0.2937727
Control:C57-LPS+NIG:APOE4 -1486.9600000 -3318.8820 344.961967 0.2664821
ATP:C57-LPS+NIG:APOE4 -1488.6166667 -3320.5386 343.305300 0.2647106
LPS:C57-LPS+NIG:APOE4 -1490.6266667 -3322.5486 341.295300 0.2625717
LPS+ATP:C57-LPS+NIG:APOE4 -1327.7500000 -3159.6720 504.171967 0.4693066
LPS+NIG:C57-LPS+NIG:APOE4 -1274.2633333 -3106.1853 557.658633 0.5477030
NIG:C57-LPS+NIG:APOE4 -1496.2366667 -3328.1586 335.685300 0.2566628
Control:NULL-LPS+NIG:APOE4 -1484.6583333 -3532.8094 563.492690 0.4690640
ATP:NULL-LPS+NIG:APOE4 -1499.0166667 -3330.9386 332.905300 0.2537679
LPS:NULL-LPS+NIG:APOE4 -1493.9066667 -3325.8286 338.015300 0.2591061
LPS+ATP:NULL-LPS+NIG:APOE4 -814.3466667 -2646.2686 1017.575300 0.9858440
LPS+NIG:NULL-LPS+NIG:APOE4 -1199.6400000 -3031.5620 632.281967 0.6580615
NIG:NULL-LPS+NIG:APOE4 -1476.1666667 -3308.0886 355.755300 0.2782138
Control:C57-NIG:APOE4 -24.6666667 -1856.5886 1807.255300 1.0000000
ATP:C57-NIG:APOE4 -26.3233333 -1858.2453 1805.598633 1.0000000
LPS:C57-NIG:APOE4 -28.3333333 -1860.2553 1803.588633 1.0000000
LPS+ATP:C57-NIG:APOE4 134.5433333 -1697.3786 1966.465300 1.0000000
LPS+NIG:C57-NIG:APOE4 188.0300000 -1643.8920 2019.951967 1.0000000
NIG:C57-NIG:APOE4 -33.9433333 -1865.8653 1797.978633 1.0000000
Control:NULL-NIG:APOE4 -22.3650000 -2070.5160 2025.786024 1.0000000
ATP:NULL-NIG:APOE4 -36.7233333 -1868.6453 1795.198633 1.0000000
LPS:NULL-NIG:APOE4 -31.6133333 -1863.5353 1800.308633 1.0000000
LPS+ATP:NULL-NIG:APOE4 647.9466667 -1183.9753 2479.868633 0.9992730
LPS+NIG:NULL-NIG:APOE4 262.6533333 -1569.2686 2094.575300 1.0000000
NIG:NULL-NIG:APOE4 -13.8733333 -1845.7953 1818.048633 1.0000000
ATP:C57-Control:C57 -1.6566667 -1833.5786 1830.265300 1.0000000
LPS:C57-Control:C57 -3.6666667 -1835.5886 1828.255300 1.0000000
LPS+ATP:C57-Control:C57 159.2100000 -1672.7120 1991.131967 1.0000000
LPS+NIG:C57-Control:C57 212.6966667 -1619.2253 2044.618633 1.0000000
NIG:C57-Control:C57 -9.2766667 -1841.1986 1822.645300 1.0000000
Control:NULL-Control:C57 2.3016667 -2045.8494 2050.452690 1.0000000
ATP:NULL-Control:C57 -12.0566667 -1843.9786 1819.865300 1.0000000
LPS:NULL-Control:C57 -6.9466667 -1838.8686 1824.975300 1.0000000
LPS+ATP:NULL-Control:C57 672.6133333 -1159.3086 2504.535300 0.9987591
LPS+NIG:NULL-Control:C57 287.3200000 -1544.6020 2119.241967 1.0000000
NIG:NULL-Control:C57 10.7933333 -1821.1286 1842.715300 1.0000000
LPS:C57-ATP:C57 -2.0100000 -1833.9320 1829.911967 1.0000000
LPS+ATP:C57-ATP:C57 160.8666667 -1671.0553 1992.788633 1.0000000
LPS+NIG:C57-ATP:C57 214.3533333 -1617.5686 2046.275300 1.0000000
NIG:C57-ATP:C57 -7.6200000 -1839.5420 1824.301967 1.0000000
Control:NULL-ATP:C57 3.9583333 -2044.1927 2052.109357 1.0000000
ATP:NULL-ATP:C57 -10.4000000 -1842.3220 1821.521967 1.0000000
LPS:NULL-ATP:C57 -5.2900000 -1837.2120 1826.631967 1.0000000
LPS+ATP:NULL-ATP:C57 674.2700000 -1157.6520 2506.191967 0.9987155
LPS+NIG:NULL-ATP:C57 288.9766667 -1542.9453 2120.898633 1.0000000
NIG:NULL-ATP:C57 12.4500000 -1819.4720 1844.371967 1.0000000
LPS+ATP:C57-LPS:C57 162.8766667 -1669.0453 1994.798633 1.0000000
LPS+NIG:C57-LPS:C57 216.3633333 -1615.5586 2048.285300 1.0000000
NIG:C57-LPS:C57 -5.6100000 -1837.5320 1826.311967 1.0000000
Control:NULL-LPS:C57 5.9683333 -2042.1827 2054.119357 1.0000000
ATP:NULL-LPS:C57 -8.3900000 -1840.3120 1823.531967 1.0000000
LPS:NULL-LPS:C57 -3.2800000 -1835.2020 1828.641967 1.0000000
LPS+ATP:NULL-LPS:C57 676.2800000 -1155.6420 2508.201967 0.9986608
LPS+NIG:NULL-LPS:C57 290.9866667 -1540.9353 2122.908633 1.0000000
NIG:NULL-LPS:C57 14.4600000 -1817.4620 1846.381967 1.0000000
LPS+NIG:C57-LPS+ATP:C57 53.4866667 -1778.4353 1885.408633 1.0000000
NIG:C57-LPS+ATP:C57 -168.4866667 -2000.4086 1663.435300 1.0000000
Control:NULL-LPS+ATP:C57 -156.9083333 -2205.0594 1891.242690 1.0000000
ATP:NULL-LPS+ATP:C57 -171.2666667 -2003.1886 1660.655300 1.0000000
LPS:NULL-LPS+ATP:C57 -166.1566667 -1998.0786 1665.765300 1.0000000
LPS+ATP:NULL-LPS+ATP:C57 513.4033333 -1318.5186 2345.325300 0.9999821
LPS+NIG:NULL-LPS+ATP:C57 128.1100000 -1703.8120 1960.031967 1.0000000
NIG:NULL-LPS+ATP:C57 -148.4166667 -1980.3386 1683.505300 1.0000000
NIG:C57-LPS+NIG:C57 -221.9733333 -2053.8953 1609.948633 1.0000000
Control:NULL-LPS+NIG:C57 -210.3950000 -2258.5460 1837.756024 1.0000000
ATP:NULL-LPS+NIG:C57 -224.7533333 -2056.6753 1607.168633 1.0000000
LPS:NULL-LPS+NIG:C57 -219.6433333 -2051.5653 1612.278633 1.0000000
LPS+ATP:NULL-LPS+NIG:C57 459.9166667 -1372.0053 2291.838633 0.9999974
LPS+NIG:NULL-LPS+NIG:C57 74.6233333 -1757.2986 1906.545300 1.0000000
NIG:NULL-LPS+NIG:C57 -201.9033333 -2033.8253 1630.018633 1.0000000
Control:NULL-NIG:C57 11.5783333 -2036.5727 2059.729357 1.0000000
ATP:NULL-NIG:C57 -2.7800000 -1834.7020 1829.141967 1.0000000
LPS:NULL-NIG:C57 2.3300000 -1829.5920 1834.251967 1.0000000
LPS+ATP:NULL-NIG:C57 681.8900000 -1150.0320 2513.811967 0.9984973
LPS+NIG:NULL-NIG:C57 296.5966667 -1535.3253 2128.518633 1.0000000
NIG:NULL-NIG:C57 20.0700000 -1811.8520 1851.991967 1.0000000
ATP:NULL-Control:NULL -14.3583333 -2062.5094 2033.792690 1.0000000
LPS:NULL-Control:NULL -9.2483333 -2057.3994 2038.902690 1.0000000
LPS+ATP:NULL-Control:NULL 670.3116667 -1377.8394 2718.462690 0.9997737
LPS+NIG:NULL-Control:NULL 285.0183333 -1763.1327 2333.169357 1.0000000
NIG:NULL-Control:NULL 8.4916667 -2039.6594 2056.642690 1.0000000
LPS:NULL-ATP:NULL 5.1100000 -1826.8120 1837.031967 1.0000000
LPS+ATP:NULL-ATP:NULL 684.6700000 -1147.2520 2516.591967 0.9984102
LPS+NIG:NULL-ATP:NULL 299.3766667 -1532.5453 2131.298633 1.0000000
NIG:NULL-ATP:NULL 22.8500000 -1809.0720 1854.771967 1.0000000
LPS+ATP:NULL-LPS:NULL 679.5600000 -1152.3620 2511.481967 0.9985672
LPS+NIG:NULL-LPS:NULL 294.2666667 -1537.6553 2126.188633 1.0000000
NIG:NULL-LPS:NULL 17.7400000 -1814.1820 1849.661967 1.0000000
LPS+NIG:NULL-LPS+ATP:NULL -385.2933333 -2217.2153 1446.628633 0.9999999
NIG:NULL-LPS+ATP:NULL -661.8200000 -2493.7420 1170.101967 0.9990134
NIG:NULL-LPS+NIG:NULL -276.5266667 -2108.4486 1555.395300 1.0000000