Introduction to Spatial omic analyses

Stefano Mangiola, South Australian immunoGENomics Cancer Institute1, Walter and Eliza Hall Institute2

Luciano Martellotto, Adelaide Centre for Epigenetics, South Australian immunoGENomics Cancer Institute3

Instructors

Dr. Stefano Mangiola is leading the Computational Cancer immunology group at the South Australian immunoGENomics Cancer Institute (SAiGENCI). He uses single-cell and spatial technologies to investigate the tumor microenvironment and the immune system. Beyong data production, his focus in on the integration and modelling of large-scale single-cell data resources. He is the author of tidytranscriptiomics and co-leads the tidyomics endevour.

BLUESKY: https://bsky.app/profile/stemang.bsky.social TWITTER/X: https://x.com/steman_research

Dr. Luciano Martelotto is a key figure in the field of spatial omics technology. He was previously the Scientific Director at the Single Cell Core facility at Harvard University, where he demonstrated his extensive expertise and significant contributions to the fields of single cell and spatial omics technology. Currently, he heads the Martelotto Lab located at the Adelaide Centre for Epigenetics and the South Australian immunoGENomics Cancer Institute (SAiGENCI). His lab is dedicated to the development and evaluation of new tools and methodologies for single cell and spatial omics, serving as an important incubator for innovation in these technologies. The lab engages in global collaborations and provides comprehensive support from the conceptual stage through to the analysis and delivery of results.

Workshop goals and objectives

What you will learn

Getting started

Local

You can view the material at the workshop webpage

here.

Workshop package installation

If you want to install the packages and material post-workshop, the instructions are below. The workshop is designed for R 4.4 and Bioconductor 3.19.

# Install workshop package
#install.packages('BiocManager')
BiocManager::install("tidyomics/tidySpatialWorkshop2024", dependencies = TRUE)

# In May 2024, the following packages should be installed from github repositories, to use the latest features. In case you have them pre installed, run the following command
BiocManager::install(c("lmweber/ggspavis", 
                       "stemangiola/tidySummarizedExperiment", 
                       "william-hutchison/tidySpatialExperiment", 
                       "stemangiola/tidybulk", 
                       "stemangiola/tidygate", 
                       "stemangiola/CuratedAtlasQueryR"), 
                     update = FALSE)
                     
BiocManager::install("ggcorrplot")
    
# Then build the vignettes
BiocManager::install("tidyomics/tidySpatialWorkshop2024", build_vignettes = TRUE, force=TRUE)

# To view vignette
library(spatialOmicsWorkshop2024)
vignette("Introduction")

Interactive execution of the vignettes

From command line, and enter the tidySpatialWorkshop2024 directory.

# Open the command line
git clone git@github.com:tidyomics/tidySpatialWorkshop2024.git

Alternatively download the git zipped package. Uncompress it. And enter the directory.

Announcements

Postdoc, RA and PhD positions available!

Introduction to Spatial Omics

Objective

Provide a foundational understanding of spatial omics, covering different technologies and the distinctions between imaging and sequencing in experimental and analytical contexts.

Workshop Structure

1. Welcome and Introduction

  • Overview of the workshop.
  • Goals for Day 1.

2. What is Spatial Omics?

  • Definition and significance in modern biology.
  • Key applications and impact.

3. Technologies in Spatial Omics

  • Overview of different spatial omics technologies.
  • Comparison of imaging-based vs sequencing-based approaches.

4. Sequencing Spatial Omics

  • Detailed comparison of methodologies.
  • Experimental design considerations.
  • Data analysis challenges and solutions.

5. Overview of Analysis Frameworks

  • Introduction to various analysis frameworks.
  • Brief mention of ‘tidy’ data principles in spatial omics.

6. Wrap-Up and Q&A

  • Summarize key takeaways.
  • Open floor for questions and discussions.

  1. ↩︎

  2. <mangiola.s at wehi.edu.au>↩︎

  3. ↩︎