CC34 Analysis

Author

LTO

Analytic Plan

flowchart LR
A[Selection into cohort] -.  ?  .-> B[Survival];

1. Flowchart to help understand sample sizes and selection

Gene expression?
Yes=1, No=0

Include reasons for why patients did not have gene expression data (MSK didn’t send, RNA extraction failed, Nanostring failed)

Eligible for Nanostring assay?
Overall
(N=929)

Eligible for Nanostring
Not Eligible 14 (1.5%)
Eligible 915 (98.5%)
Contrast-enhanced CT?
Overall
(N=929)

contrast
No contrast 764 (82.2%)
Contrast 165 (17.8%)
Contrast x Eligible for Nanostring
Not Eligible
(N=14)
Eligible
(N=915)
Overall
(N=929)

contrast
No contrast 10 (71.4%) 754 (82.4%) 764 (82.2%)
Contrast 4 (28.6%) 161 (17.6%) 165 (17.8%)
Has QC'd gene expression data? x Contrast
No Data
(N=92)
Has Data
(N=837)
Overall
(N=929)

contrast
No contrast 77 (83.7%) 687 (82.1%) 764 (82.2%)
Contrast 15 (16.3%) 150 (17.9%) 165 (17.8%)



Among all patients in RESOLVE:
flowchart LR

A[All RESOLVE Patients<br />n=1,239]
B[Sent to UNC<br />n=929]
C[Nanostring eligible<br />n=915]
D[Molecular data set<br />n=837]
A --> B --> C --> D



Among patients in RESOLVE with non-contrast CTs:
flowchart LR

A[All RESOLVE Patients<br />n=1,022]
B[Sent to UNC<br />n=764]
C[Nanostring eligible<br />n=754]
D[Molecular data set<br />n=687]
A --> B --> C --> D



2. Descriptive stats by whether patients have gene expression data

Characteristic Gene Expression p-value2
Overall, N = 1,2391 No gene expr data
(N = 402)1
Gene expr data
(N = 837)1
Age (cnts) 58 (49, 66) 58 (50, 66) 58 (49, 66) 0.9
Sex


0.4
    Female 388 (31%) 119 (30%) 269 (32%)
    Male 851 (69%) 283 (70%) 568 (68%)
Race


0.7
    Asian 55 (4.4%) 15 (3.7%) 40 (4.8%)
    Black 36 (2.9%) 11 (2.7%) 25 (3.0%)
    Other 17 (1.4%) 7 (1.7%) 10 (1.2%)
    Unknown 28 (2.3%) 7 (1.7%) 21 (2.5%)
    White 1,103 (89%) 362 (90%) 741 (89%)
Tumor grade


0.11
    1 29 (2.3%) 15 (3.7%) 14 (1.7%)
    2 582 (47%) 180 (45%) 402 (48%)
    3 533 (43%) 173 (43%) 360 (43%)
    4 95 (7.7%) 34 (8.5%) 61 (7.3%)
Tumor stage


0.028
    1 795 (64%) 248 (62%) 547 (65%)
    2 63 (5.1%) 30 (7.5%) 33 (3.9%)
    3 381 (31%) 124 (31%) 257 (31%)
Tumor size 4.00 (2.50, 6.00) 4.00 (2.50, 6.50) 4.00 (2.50, 6.00) 0.5
Ever smoked


>0.9
    Ever 604 (49%) 197 (49%) 407 (49%)
    Never 635 (51%) 205 (51%) 430 (51%)
BMI 29.6 (26.4, 33.4) 29.4 (26.2, 33.5) 29.6 (26.6, 33.4) 0.5
History of diabetes 193 (16%) 53 (13%) 140 (17%) 0.11
History of hypertension 674 (54%) 217 (54%) 457 (55%) 0.8
History of hyperlipidemia 469 (38%) 147 (37%) 322 (38%) 0.5
CKD at baseline


0.3
    CKD 239 (19%) 84 (21%) 155 (19%)
    No CKD 1,000 (81%) 318 (79%) 682 (81%)
SMI 54 (46, 61) 54 (46, 61) 54 (46, 61) >0.9
SMD 38 (32, 43) 38 (32, 44) 37 (32, 43) 0.3
VATI 64 (41, 90) 62 (40, 86) 66 (42, 92) 0.090
VATD -96 (-100, -91) -96 (-100, -91) -96 (-100, -91) 0.2
SATI 73 (52, 105) 72 (52, 104) 74 (52, 106) 0.5
SATD -101 (-105, -97) -101 (-105, -97) -102 (-106, -98) 0.092
1 Median (IQR); n (%)
2 Wilcoxon rank sum test; Pearson’s Chi-squared test


Characteristic Gene Expression among non-contrast p-value2
Overall, N = 1,0221 No gene expr data
(N = 335)1
Gene expr data
(N = 687)1
Age (cnts) 58 (49, 66) 58 (50, 66) 58 (49, 66) 0.5
Sex


0.3
    Female 320 (31%) 97 (29%) 223 (32%)
    Male 702 (69%) 238 (71%) 464 (68%)
Race


0.5
    Asian 43 (4.2%) 10 (3.0%) 33 (4.8%)
    Black 32 (3.1%) 9 (2.7%) 23 (3.3%)
    Other 12 (1.2%) 5 (1.5%) 7 (1.0%)
    Unknown 24 (2.3%) 6 (1.8%) 18 (2.6%)
    White 911 (89%) 305 (91%) 606 (88%)
Tumor grade


0.5
    1 26 (2.5%) 12 (3.6%) 14 (2.0%)
    2 481 (47%) 155 (46%) 326 (47%)
    3 435 (43%) 141 (42%) 294 (43%)
    4 80 (7.8%) 27 (8.1%) 53 (7.7%)
Tumor stage


0.049
    1 658 (64%) 207 (62%) 451 (66%)
    2 52 (5.1%) 25 (7.5%) 27 (3.9%)
    3 312 (31%) 103 (31%) 209 (30%)
Tumor size 3.95 (2.50, 6.00) 4.00 (2.50, 6.50) 3.90 (2.50, 5.90) 0.6
Ever smoked


0.4
    Ever 501 (49%) 170 (51%) 331 (48%)
    Never 521 (51%) 165 (49%) 356 (52%)
BMI 29.7 (26.5, 33.6) 29.4 (26.2, 33.7) 29.8 (26.6, 33.6) 0.5
History of diabetes 163 (16%) 44 (13%) 119 (17%) 0.086
History of hypertension 567 (55%) 185 (55%) 382 (56%) >0.9
History of hyperlipidemia 390 (38%) 122 (36%) 268 (39%) 0.4
CKD at baseline


0.2
    CKD 208 (20%) 76 (23%) 132 (19%)
    No CKD 814 (80%) 259 (77%) 555 (81%)
SMI 53 (46, 61) 53 (46, 61) 53 (46, 62) 0.7
SMD 37 (31, 43) 38 (31, 43) 37 (31, 43) 0.7
VATI 65 (43, 91) 63 (43, 86) 66 (43, 93) 0.2
VATD -97 (-100, -92) -97 (-100, -92) -97 (-101, -92) 0.3
SATI 73 (52, 105) 71 (52, 103) 74 (52, 107) 0.5
SATD -102 (-106, -98) -101 (-105, -97) -102 (-106, -98) 0.053
1 Median (IQR); n (%)
2 Wilcoxon rank sum test; Pearson’s Chi-squared test; Fisher’s exact test



3. KM Plots and Cox PH models by whether patients have gene expression data available


Characteristic HR1 95% CI1 p-value
Gene Expr Data DFS 0.77 0.55, 1.06 0.11
Gene Expr Data OS 0.82 0.50, 1.35 0.4
Gene Expr Data DFS (non-contrast) 0.85 0.60, 1.22 0.4
Gene Expr Data OS (non-contrast) 1.03 0.59, 1.78 >0.9
1 HR = Hazard Ratio, CI = Confidence Interval