Results interpretations

Nothing is significant after correcting for multiple comparisons, so weak to no association between EED and stress outcome

Looks like Alpha-1 antitrypsan has the strongest associations with stress - specifically cortisol and salivary amalyase, but in opposite directions. I think cortisol and amalyase should both increase with stress, so interesting and hard to interpret finding. Ask Audrie, but could be random chance.

Figures

Consort figure

(to add)

Heatmaps - adjusted analyses

Primary figures To do: scale point estimates, and add figure legends

Figure 1 -Heatmap of adjusted association’s direction and significance for year 1 measurements

Cells are colored by the direction and P-value significance for each EED exposure and stress outcome combination. Printed in each cell is the point estimate from adjusted regression models. Each row is a specific stress outcome and each column is an EED exposure.

Figure 2 -Heatmap of adjusted association’s direction and significance for year 2 measurements

Cells are colored by the direction and P-value significance for each EED exposure and stress outcome combination. Printed in each cell is the point estimate from adjusted regression models. Each row is a specific stress outcome and each column is an EED exposure.

Heatmaps - unadjusted analyses

-supplimentary figures

Forest plots

(adjusted results only) To do: format labels

Spline plots

Supplementary plots: to add for key significant relationships

Tables

-Note I’m splitting the tables by domain of stress outcome (consistent with prior stress papers from this study)

EED biomarker adjusted associations with year-1 oxidative stress measures

Biomarkers measured at child age 14 months

EED exposure Stress outcome Meas. unit Estimate P-value FDR-corrected P-value
AAT iPF(2a)-III ng/mg creatinine 0.009 (-0.045, 0.064) 0.7376 0.7696733
AAT 2,3-dinor-iPF(2a)-III ng/mg creatinine -0.012 (-0.054, 0.031) 0.5895 0.7074301
AAT iPF(2a)-VI ng/mg creatinine 0.012 (-0.050, 0.074) 0.7043 0.7696733
AAT 8,12-iso-iPF(2a)-VI ng/mg creatinine 0.012 (-0.058, 0.083) 0.7300 0.7696733
MPO iPF(2a)-III ng/mg creatinine -0.017 (-0.065, 0.030) 0.4756 0.6664969
MPO 2,3-dinor-iPF(2a)-III ng/mg creatinine -0.017 (-0.048, 0.013) 0.2626 0.6303269
MPO iPF(2a)-VI ng/mg creatinine -0.015 (-0.063, 0.032) 0.5276 0.6664969
MPO 8,12-iso-iPF(2a)-VI ng/mg creatinine -0.027 (-0.082, 0.027) 0.3265 0.6664969
NEO iPF(2a)-III ng/mg creatinine -0.045 (-0.118, 0.027) 0.2215 0.6303269
NEO 2,3-dinor-iPF(2a)-III ng/mg creatinine -0.046 (-0.104, 0.013) 0.1262 0.6303269
NEO iPF(2a)-VI ng/mg creatinine -0.028 (-0.106, 0.050) 0.4870 0.6664969
NEO 8,12-iso-iPF(2a)-VI ng/mg creatinine -0.045 (-0.142, 0.052) 0.3615 0.6664969
Lact iPF(2a)-III ng/mg creatinine 0.015 (-0.028, 0.057) 0.5019 0.6664969
Lact 2,3-dinor-iPF(2a)-III ng/mg creatinine 0.019 (-0.009, 0.047) 0.1760 0.6303269
Lact iPF(2a)-VI ng/mg creatinine -0.004 (-0.039, 0.032) 0.8418 0.8418374
Lact 8,12-iso-iPF(2a)-VI ng/mg creatinine 0.022 (-0.023, 0.067) 0.3336 0.6664969
Mann iPF(2a)-III ng/mg creatinine 0.036 (-0.010, 0.082) 0.1231 0.6303269
Mann 2,3-dinor-iPF(2a)-III ng/mg creatinine 0.022 (-0.009, 0.054) 0.1689 0.6303269
Mann iPF(2a)-VI ng/mg creatinine 0.016 (-0.023, 0.056) 0.4206 0.6664969
Mann 8,12-iso-iPF(2a)-VI ng/mg creatinine 0.056 (0.005, 0.108) 0.0313 0.6303269
REG1B iPF(2a)-III ng/mg creatinine -0.027 (-0.071, 0.018) 0.2433 0.6303269
REG1B 2,3-dinor-iPF(2a)-III ng/mg creatinine 0.009 (-0.016, 0.033) 0.4825 0.6664969
REG1B iPF(2a)-VI ng/mg creatinine -0.023 (-0.059, 0.012) 0.1957 0.6303269
REG1B 8,12-iso-iPF(2a)-VI ng/mg creatinine -0.030 (-0.077, 0.017) 0.2105 0.6303269

EED biomarker adjusted associations with year-2 sympathetic adrenomedullary axis measures

EED exposure Stress outcome Meas. unit Estimate P-value FDR-corrected P-value
AAT Mean arterial pressure mmHg 0.086 (-0.257, 0.429) 0.6224 0.6819064
AAT Resting heart rate bpm 0.787 (-0.157, 1.732) 0.1023 0.3720139
AAT Slope between pre- and post-stressor alpha-amylase U/ml/min -0.513 (-0.931, -0.096) 0.0159 0.1305711
AAT Residualized gain score for alpha-amylase U/ml -7.998 (-14.856, -1.139) 0.0223 0.1305711
MPO Mean arterial pressure mmHg 0.294 (-0.030, 0.617) 0.0749 0.2997172
MPO Resting heart rate bpm -0.277 (-1.210, 0.657) 0.5612 0.6609979
MPO Slope between pre- and post-stressor alpha-amylase U/ml/min 0.189 (-0.375, 0.754) 0.5110 0.6609979
MPO Residualized gain score for alpha-amylase U/ml 5.796 (-4.033, 15.624) 0.2478 0.5332721
NEO Mean arterial pressure mmHg 0.515 (0.130, 0.900) 0.0088 0.1305711
NEO Resting heart rate bpm 0.527 (-0.493, 1.546) 0.3113 0.5413121
NEO Slope between pre- and post-stressor alpha-amylase U/ml/min 0.292 (-0.231, 0.814) 0.2737 0.5332721
NEO Residualized gain score for alpha-amylase U/ml 5.113 (-3.576, 13.801) 0.2488 0.5332721
Lact Mean arterial pressure mmHg 0.122 (-0.183, 0.427) 0.4340 0.6609979
Lact Resting heart rate bpm -0.588 (-1.313, 0.137) 0.1120 0.3734191
Lact Slope between pre- and post-stressor alpha-amylase U/ml/min -0.077 (-0.388, 0.234) 0.6281 0.6819064
Lact Residualized gain score for alpha-amylase U/ml -1.592 (-6.769, 3.585) 0.5467 0.6609979
Mann Mean arterial pressure mmHg 0.083 (-0.257, 0.424) 0.6308 0.6819064
Mann Resting heart rate bpm -0.768 (-1.548, 0.013) 0.0538 0.2689487
Mann Slope between pre- and post-stressor alpha-amylase U/ml/min -0.011 (-0.307, 0.286) 0.9442 0.9441557
Mann Residualized gain score for alpha-amylase U/ml -0.508 (-5.628, 4.612) 0.8458 0.8902763

EED biomarker adjusted associations with year-2 hypothalamic-pituitary-adrenal axis measures

EED exposure Stress outcome Meas. unit Estimate P-value FDR-corrected P-value
AAT NR3C1 exon 1F promoter methylation -0.042 (-0.101, 0.016) 0.1553 0.4725492
AAT NGFI-A transcription factor binding site methylation 0.012 (-0.029, 0.053) 0.5618 0.6609979
AAT Slope between pre- and post-stressor cortisol μg/dl/min 0.001 (0.000, 0.001) 0.0213 0.1305711
AAT Residualized gain score for cortisol μg/dl 0.024 (0.005, 0.043) 0.0157 0.1305711
MPO NR3C1 exon 1F promoter methylation 0.003 (-0.055, 0.062) 0.9086 0.9319487
MPO NGFI-A transcription factor binding site methylation -0.047 (-0.095, 0.002) 0.0619 0.2752044
MPO Slope between pre- and post-stressor cortisol μg/dl/min -0.000 (-0.001, 0.000) 0.3464 0.5773537
MPO Residualized gain score for cortisol μg/dl -0.009 (-0.034, 0.015) 0.4563 0.6609979
NEO NR3C1 exon 1F promoter methylation -0.035 (-0.112, 0.042) 0.3728 0.5964938
NEO NGFI-A transcription factor binding site methylation 0.016 (-0.031, 0.063) 0.4995 0.6609979
NEO Slope between pre- and post-stressor cortisol μg/dl/min 0.000 (-0.000, 0.001) 0.2902 0.5332721
NEO Residualized gain score for cortisol μg/dl 0.014 (-0.009, 0.038) 0.2348 0.5332721
Lact NR3C1 exon 1F promoter methylation 0.066 (0.016, 0.116) 0.0102 0.1305711
Lact NGFI-A transcription factor binding site methylation 0.016 (-0.028, 0.060) 0.4778 0.6609979
Lact Slope between pre- and post-stressor cortisol μg/dl/min -0.000 (-0.001, 0.000) 0.2745 0.5332721
Lact Residualized gain score for cortisol μg/dl -0.011 (-0.031, 0.009) 0.2933 0.5332721
Mann NR3C1 exon 1F promoter methylation 0.060 (0.008, 0.111) 0.0228 0.1305711
Mann NGFI-A transcription factor binding site methylation 0.013 (-0.026, 0.052) 0.5173 0.6609979
Mann Slope between pre- and post-stressor cortisol μg/dl/min -0.001 (-0.001, 0.000) 0.1654 0.4725492
Mann Residualized gain score for cortisol μg/dl -0.015 (-0.038, 0.009) 0.2273 0.5332721