birthweight <- read.csv("birthweight.csv")
mir <- read.csv("miRNA.csv", row.names = 1)
experiment <- merge(birthweight, mir)
experiment
##       ID birth.date    location length birthweight head.circumference
## 1   1107  1/25/1967     General     52        3.23                 36
## 2    697   2/6/1967 Silver Hill     48        3.03                 35
## 3   1683  2/14/1967 Silver Hill     53        3.35                 33
## 4     27   3/9/1967 Silver Hill     53        3.55                 37
## 5   1522  3/13/1967    Memorial     50        2.74                 33
## 6    569  3/23/1967    Memorial     50        2.51                 35
## 7    365  4/23/1967    Memorial     52        3.53                 37
## 8    808   5/5/1967 Silver Hill     48        2.92                 33
## 9   1369   6/4/1967 Silver Hill     49        3.18                 34
## 10  1023   6/7/1967    Memorial     52        3.00                 35
## 11   822  6/14/1967    Memorial     50        3.42                 35
## 12  1272  6/20/1967    Memorial     53        2.75                 32
## 13  1262  6/25/1967 Silver Hill     53        3.19                 34
## 14   575  7/12/1967    Memorial     50        2.78                 30
## 15  1016  7/13/1967 Silver Hill     53        4.32                 36
## 16   792   9/7/1967    Memorial     53        3.64                 38
## 17   820  10/7/1967     General     52        3.77                 34
## 18   752 10/19/1967     General     49        3.32                 36
## 19   619  11/1/1967    Memorial     52        3.41                 33
## 20  1764  12/7/1967 Silver Hill     58        4.57                 39
## 21  1081 12/14/1967 Silver Hill     54        3.63                 38
## 22   516   1/8/1968 Silver Hill     47        2.66                 33
## 23   272  1/10/1968    Memorial     52        3.86                 36
## 24   321  1/21/1968 Silver Hill     48        3.11                 33
## 25  1636   2/2/1968 Silver Hill     51        3.93                 38
## 26  1360  2/16/1968     General     56        4.55                 34
## 27  1388  2/22/1968    Memorial     51        3.14                 33
## 28  1363   4/2/1968     General     48        2.37                 30
## 29  1058  4/24/1968 Silver Hill     53        3.15                 34
## 30   755  4/25/1968    Memorial     53        3.20                 33
## 31   462  6/19/1968 Silver Hill     58        4.10                 39
## 32   300  7/18/1968 Silver Hill     46        2.05                 32
## 33  1088  7/24/1968     General     51        3.27                 36
## 34    57  8/12/1968    Memorial     51        3.32                 38
## 35   553  8/17/1968 Silver Hill     54        3.94                 37
## 36  1191   9/7/1968     General     53        3.65                 33
## 37   431  9/16/1968 Silver Hill     48        1.92                 30
## 38  1313  9/27/1968 Silver Hill     43        2.65                 32
## 39  1600  10/9/1968     General     53        2.90                 34
## 40   532 10/25/1968     General     53        3.59                 34
## 41   223 12/11/1968     General     50        3.87                 33
## 42  1187 12/19/1968 Silver Hill     53        4.07                 38
## 43  1107  1/25/1967     General     52        3.23                 36
## 44   697   2/6/1967 Silver Hill     48        3.03                 35
## 45  1683  2/14/1967 Silver Hill     53        3.35                 33
## 46    27   3/9/1967 Silver Hill     53        3.55                 37
## 47  1522  3/13/1967    Memorial     50        2.74                 33
## 48   569  3/23/1967    Memorial     50        2.51                 35
## 49   365  4/23/1967    Memorial     52        3.53                 37
## 50   808   5/5/1967 Silver Hill     48        2.92                 33
## 51  1369   6/4/1967 Silver Hill     49        3.18                 34
## 52  1023   6/7/1967    Memorial     52        3.00                 35
## 53   822  6/14/1967    Memorial     50        3.42                 35
## 54  1272  6/20/1967    Memorial     53        2.75                 32
## 55  1262  6/25/1967 Silver Hill     53        3.19                 34
## 56   575  7/12/1967    Memorial     50        2.78                 30
## 57  1016  7/13/1967 Silver Hill     53        4.32                 36
## 58   792   9/7/1967    Memorial     53        3.64                 38
## 59   820  10/7/1967     General     52        3.77                 34
## 60   752 10/19/1967     General     49        3.32                 36
## 61   619  11/1/1967    Memorial     52        3.41                 33
## 62  1764  12/7/1967 Silver Hill     58        4.57                 39
## 63  1081 12/14/1967 Silver Hill     54        3.63                 38
## 64   516   1/8/1968 Silver Hill     47        2.66                 33
## 65   272  1/10/1968    Memorial     52        3.86                 36
## 66   321  1/21/1968 Silver Hill     48        3.11                 33
## 67  1636   2/2/1968 Silver Hill     51        3.93                 38
## 68  1360  2/16/1968     General     56        4.55                 34
## 69  1388  2/22/1968    Memorial     51        3.14                 33
## 70  1363   4/2/1968     General     48        2.37                 30
## 71  1058  4/24/1968 Silver Hill     53        3.15                 34
## 72   755  4/25/1968    Memorial     53        3.20                 33
## 73   462  6/19/1968 Silver Hill     58        4.10                 39
## 74   300  7/18/1968 Silver Hill     46        2.05                 32
## 75  1088  7/24/1968     General     51        3.27                 36
## 76    57  8/12/1968    Memorial     51        3.32                 38
## 77   553  8/17/1968 Silver Hill     54        3.94                 37
## 78  1191   9/7/1968     General     53        3.65                 33
## 79   431  9/16/1968 Silver Hill     48        1.92                 30
## 80  1313  9/27/1968 Silver Hill     43        2.65                 32
## 81  1600  10/9/1968     General     53        2.90                 34
## 82   532 10/25/1968     General     53        3.59                 34
## 83   223 12/11/1968     General     50        3.87                 33
## 84  1187 12/19/1968 Silver Hill     53        4.07                 38
## 85  1107  1/25/1967     General     52        3.23                 36
## 86   697   2/6/1967 Silver Hill     48        3.03                 35
## 87  1683  2/14/1967 Silver Hill     53        3.35                 33
## 88    27   3/9/1967 Silver Hill     53        3.55                 37
## 89  1522  3/13/1967    Memorial     50        2.74                 33
## 90   569  3/23/1967    Memorial     50        2.51                 35
## 91   365  4/23/1967    Memorial     52        3.53                 37
## 92   808   5/5/1967 Silver Hill     48        2.92                 33
## 93  1369   6/4/1967 Silver Hill     49        3.18                 34
## 94  1023   6/7/1967    Memorial     52        3.00                 35
## 95   822  6/14/1967    Memorial     50        3.42                 35
## 96  1272  6/20/1967    Memorial     53        2.75                 32
## 97  1262  6/25/1967 Silver Hill     53        3.19                 34
## 98   575  7/12/1967    Memorial     50        2.78                 30
## 99  1016  7/13/1967 Silver Hill     53        4.32                 36
## 100  792   9/7/1967    Memorial     53        3.64                 38
## 101  820  10/7/1967     General     52        3.77                 34
## 102  752 10/19/1967     General     49        3.32                 36
## 103  619  11/1/1967    Memorial     52        3.41                 33
## 104 1764  12/7/1967 Silver Hill     58        4.57                 39
## 105 1081 12/14/1967 Silver Hill     54        3.63                 38
## 106  516   1/8/1968 Silver Hill     47        2.66                 33
## 107  272  1/10/1968    Memorial     52        3.86                 36
## 108  321  1/21/1968 Silver Hill     48        3.11                 33
## 109 1636   2/2/1968 Silver Hill     51        3.93                 38
## 110 1360  2/16/1968     General     56        4.55                 34
## 111 1388  2/22/1968    Memorial     51        3.14                 33
## 112 1363   4/2/1968     General     48        2.37                 30
## 113 1058  4/24/1968 Silver Hill     53        3.15                 34
## 114  755  4/25/1968    Memorial     53        3.20                 33
## 115  462  6/19/1968 Silver Hill     58        4.10                 39
## 116  300  7/18/1968 Silver Hill     46        2.05                 32
## 117 1088  7/24/1968     General     51        3.27                 36
## 118   57  8/12/1968    Memorial     51        3.32                 38
## 119  553  8/17/1968 Silver Hill     54        3.94                 37
## 120 1191   9/7/1968     General     53        3.65                 33
## 121  431  9/16/1968 Silver Hill     48        1.92                 30
## 122 1313  9/27/1968 Silver Hill     43        2.65                 32
## 123 1600  10/9/1968     General     53        2.90                 34
## 124  532 10/25/1968     General     53        3.59                 34
## 125  223 12/11/1968     General     50        3.87                 33
## 126 1187 12/19/1968 Silver Hill     53        4.07                 38
## 127 1107  1/25/1967     General     52        3.23                 36
## 128  697   2/6/1967 Silver Hill     48        3.03                 35
## 129 1683  2/14/1967 Silver Hill     53        3.35                 33
## 130   27   3/9/1967 Silver Hill     53        3.55                 37
## 131 1522  3/13/1967    Memorial     50        2.74                 33
## 132  569  3/23/1967    Memorial     50        2.51                 35
## 133  365  4/23/1967    Memorial     52        3.53                 37
## 134  808   5/5/1967 Silver Hill     48        2.92                 33
## 135 1369   6/4/1967 Silver Hill     49        3.18                 34
## 136 1023   6/7/1967    Memorial     52        3.00                 35
## 137  822  6/14/1967    Memorial     50        3.42                 35
## 138 1272  6/20/1967    Memorial     53        2.75                 32
## 139 1262  6/25/1967 Silver Hill     53        3.19                 34
## 140  575  7/12/1967    Memorial     50        2.78                 30
## 141 1016  7/13/1967 Silver Hill     53        4.32                 36
## 142  792   9/7/1967    Memorial     53        3.64                 38
## 143  820  10/7/1967     General     52        3.77                 34
## 144  752 10/19/1967     General     49        3.32                 36
## 145  619  11/1/1967    Memorial     52        3.41                 33
## 146 1764  12/7/1967 Silver Hill     58        4.57                 39
## 147 1081 12/14/1967 Silver Hill     54        3.63                 38
## 148  516   1/8/1968 Silver Hill     47        2.66                 33
## 149  272  1/10/1968    Memorial     52        3.86                 36
## 150  321  1/21/1968 Silver Hill     48        3.11                 33
## 151 1636   2/2/1968 Silver Hill     51        3.93                 38
## 152 1360  2/16/1968     General     56        4.55                 34
## 153 1388  2/22/1968    Memorial     51        3.14                 33
## 154 1363   4/2/1968     General     48        2.37                 30
## 155 1058  4/24/1968 Silver Hill     53        3.15                 34
## 156  755  4/25/1968    Memorial     53        3.20                 33
## 157  462  6/19/1968 Silver Hill     58        4.10                 39
## 158  300  7/18/1968 Silver Hill     46        2.05                 32
## 159 1088  7/24/1968     General     51        3.27                 36
## 160   57  8/12/1968    Memorial     51        3.32                 38
## 161  553  8/17/1968 Silver Hill     54        3.94                 37
## 162 1191   9/7/1968     General     53        3.65                 33
## 163  431  9/16/1968 Silver Hill     48        1.92                 30
## 164 1313  9/27/1968 Silver Hill     43        2.65                 32
## 165 1600  10/9/1968     General     53        2.90                 34
## 166  532 10/25/1968     General     53        3.59                 34
## 167  223 12/11/1968     General     50        3.87                 33
## 168 1187 12/19/1968 Silver Hill     53        4.07                 38
##     weeks.gestation smoker maternal.age maternal.cigarettes maternal.height
## 1                38     no           31                   0             164
## 2                39     no           27                   0             162
## 3                41     no           27                   0             164
## 4                41    yes           37                  25             161
## 5                39    yes           21                  17             156
## 6                39    yes           22                   7             159
## 7                40    yes           26                  25             170
## 8                34     no           26                   0             167
## 9                38    yes           31                  25             162
## 10               38    yes           30                  12             165
## 11               38     no           20                   0             157
## 12               40    yes           37                  50             168
## 13               41    yes           27                  35             163
## 14               37    yes           19                   7             165
## 15               40     no           19                   0             171
## 16               40    yes           20                   2             170
## 17               40     no           24                   0             157
## 18               40    yes           27                  12             152
## 19               39    yes           23                  25             181
## 20               41    yes           32                  12             173
## 21               38     no           18                   0             172
## 22               35    yes           20                  35             170
## 23               39    yes           30                  25             170
## 24               37     no           28                   0             158
## 25               38     no           29                   0             165
## 26               44     no           20                   0             162
## 27               41    yes           22                   7             160
## 28               37    yes           20                   7             163
## 29               40     no           29                   0             167
## 30               41     no           21                   0             155
## 31               41     no           35                   0             172
## 32               35    yes           41                   7             166
## 33               40     no           24                   0             168
## 34               39    yes           23                  17             157
## 35               42     no           24                   0             175
## 36               42     no           21                   0             165
## 37               33    yes           20                   7             161
## 38               33     no           24                   0             149
## 39               39     no           19                   0             165
## 40               40    yes           31                  12             163
## 41               45    yes           28                  25             163
## 42               44     no           20                   0             174
## 43               38     no           31                   0             164
## 44               39     no           27                   0             162
## 45               41     no           27                   0             164
## 46               41    yes           37                  25             161
## 47               39    yes           21                  17             156
## 48               39    yes           22                   7             159
## 49               40    yes           26                  25             170
## 50               34     no           26                   0             167
## 51               38    yes           31                  25             162
## 52               38    yes           30                  12             165
## 53               38     no           20                   0             157
## 54               40    yes           37                  50             168
## 55               41    yes           27                  35             163
## 56               37    yes           19                   7             165
## 57               40     no           19                   0             171
## 58               40    yes           20                   2             170
## 59               40     no           24                   0             157
## 60               40    yes           27                  12             152
## 61               39    yes           23                  25             181
## 62               41    yes           32                  12             173
## 63               38     no           18                   0             172
## 64               35    yes           20                  35             170
## 65               39    yes           30                  25             170
## 66               37     no           28                   0             158
## 67               38     no           29                   0             165
## 68               44     no           20                   0             162
## 69               41    yes           22                   7             160
## 70               37    yes           20                   7             163
## 71               40     no           29                   0             167
## 72               41     no           21                   0             155
## 73               41     no           35                   0             172
## 74               35    yes           41                   7             166
## 75               40     no           24                   0             168
## 76               39    yes           23                  17             157
## 77               42     no           24                   0             175
## 78               42     no           21                   0             165
## 79               33    yes           20                   7             161
## 80               33     no           24                   0             149
## 81               39     no           19                   0             165
## 82               40    yes           31                  12             163
## 83               45    yes           28                  25             163
## 84               44     no           20                   0             174
## 85               38     no           31                   0             164
## 86               39     no           27                   0             162
## 87               41     no           27                   0             164
## 88               41    yes           37                  25             161
## 89               39    yes           21                  17             156
## 90               39    yes           22                   7             159
## 91               40    yes           26                  25             170
## 92               34     no           26                   0             167
## 93               38    yes           31                  25             162
## 94               38    yes           30                  12             165
## 95               38     no           20                   0             157
## 96               40    yes           37                  50             168
## 97               41    yes           27                  35             163
## 98               37    yes           19                   7             165
## 99               40     no           19                   0             171
## 100              40    yes           20                   2             170
## 101              40     no           24                   0             157
## 102              40    yes           27                  12             152
## 103              39    yes           23                  25             181
## 104              41    yes           32                  12             173
## 105              38     no           18                   0             172
## 106              35    yes           20                  35             170
## 107              39    yes           30                  25             170
## 108              37     no           28                   0             158
## 109              38     no           29                   0             165
## 110              44     no           20                   0             162
## 111              41    yes           22                   7             160
## 112              37    yes           20                   7             163
## 113              40     no           29                   0             167
## 114              41     no           21                   0             155
## 115              41     no           35                   0             172
## 116              35    yes           41                   7             166
## 117              40     no           24                   0             168
## 118              39    yes           23                  17             157
## 119              42     no           24                   0             175
## 120              42     no           21                   0             165
## 121              33    yes           20                   7             161
## 122              33     no           24                   0             149
## 123              39     no           19                   0             165
## 124              40    yes           31                  12             163
## 125              45    yes           28                  25             163
## 126              44     no           20                   0             174
## 127              38     no           31                   0             164
## 128              39     no           27                   0             162
## 129              41     no           27                   0             164
## 130              41    yes           37                  25             161
## 131              39    yes           21                  17             156
## 132              39    yes           22                   7             159
## 133              40    yes           26                  25             170
## 134              34     no           26                   0             167
## 135              38    yes           31                  25             162
## 136              38    yes           30                  12             165
## 137              38     no           20                   0             157
## 138              40    yes           37                  50             168
## 139              41    yes           27                  35             163
## 140              37    yes           19                   7             165
## 141              40     no           19                   0             171
## 142              40    yes           20                   2             170
## 143              40     no           24                   0             157
## 144              40    yes           27                  12             152
## 145              39    yes           23                  25             181
## 146              41    yes           32                  12             173
## 147              38     no           18                   0             172
## 148              35    yes           20                  35             170
## 149              39    yes           30                  25             170
## 150              37     no           28                   0             158
## 151              38     no           29                   0             165
## 152              44     no           20                   0             162
## 153              41    yes           22                   7             160
## 154              37    yes           20                   7             163
## 155              40     no           29                   0             167
## 156              41     no           21                   0             155
## 157              41     no           35                   0             172
## 158              35    yes           41                   7             166
## 159              40     no           24                   0             168
## 160              39    yes           23                  17             157
## 161              42     no           24                   0             175
## 162              42     no           21                   0             165
## 163              33    yes           20                   7             161
## 164              33     no           24                   0             149
## 165              39     no           19                   0             165
## 166              40    yes           31                  12             163
## 167              45    yes           28                  25             163
## 168              44     no           20                   0             174
##     maternal.prepregnant.weight paternal.age paternal.education
## 1                            57           NA                 NA
## 2                            62           27                 14
## 3                            62           37                 14
## 4                            66           46                 NA
## 5                            53           24                 12
## 6                            52           23                 14
## 7                            62           30                 10
## 8                            64           25                 12
## 9                            57           32                 16
## 10                           64           38                 14
## 11                           48           22                 14
## 12                           61           31                 16
## 13                           51           31                 16
## 14                           60           20                 14
## 15                           62           19                 12
## 16                           59           24                 12
## 17                           50           31                 16
## 18                           48           37                 12
## 19                           69           23                 16
## 20                           70           38                 14
## 21                           50           20                 12
## 22                           57           23                 12
## 23                           78           40                 16
## 24                           54           39                 10
## 25                           61           NA                 NA
## 26                           57           23                 10
## 27                           53           24                 16
## 28                           47           20                 10
## 29                           60           30                 16
## 30                           55           25                 14
## 31                           58           31                 16
## 32                           57           37                 14
## 33                           53           29                 16
## 34                           48           NA                 NA
## 35                           66           30                 12
## 36                           61           21                 10
## 37                           50           20                 10
## 38                           45           26                 16
## 39                           57           NA                 NA
## 40                           49           41                 12
## 41                           54           30                 16
## 42                           68           26                 14
## 43                           57           NA                 NA
## 44                           62           27                 14
## 45                           62           37                 14
## 46                           66           46                 NA
## 47                           53           24                 12
## 48                           52           23                 14
## 49                           62           30                 10
## 50                           64           25                 12
## 51                           57           32                 16
## 52                           64           38                 14
## 53                           48           22                 14
## 54                           61           31                 16
## 55                           51           31                 16
## 56                           60           20                 14
## 57                           62           19                 12
## 58                           59           24                 12
## 59                           50           31                 16
## 60                           48           37                 12
## 61                           69           23                 16
## 62                           70           38                 14
## 63                           50           20                 12
## 64                           57           23                 12
## 65                           78           40                 16
## 66                           54           39                 10
## 67                           61           NA                 NA
## 68                           57           23                 10
## 69                           53           24                 16
## 70                           47           20                 10
## 71                           60           30                 16
## 72                           55           25                 14
## 73                           58           31                 16
## 74                           57           37                 14
## 75                           53           29                 16
## 76                           48           NA                 NA
## 77                           66           30                 12
## 78                           61           21                 10
## 79                           50           20                 10
## 80                           45           26                 16
## 81                           57           NA                 NA
## 82                           49           41                 12
## 83                           54           30                 16
## 84                           68           26                 14
## 85                           57           NA                 NA
## 86                           62           27                 14
## 87                           62           37                 14
## 88                           66           46                 NA
## 89                           53           24                 12
## 90                           52           23                 14
## 91                           62           30                 10
## 92                           64           25                 12
## 93                           57           32                 16
## 94                           64           38                 14
## 95                           48           22                 14
## 96                           61           31                 16
## 97                           51           31                 16
## 98                           60           20                 14
## 99                           62           19                 12
## 100                          59           24                 12
## 101                          50           31                 16
## 102                          48           37                 12
## 103                          69           23                 16
## 104                          70           38                 14
## 105                          50           20                 12
## 106                          57           23                 12
## 107                          78           40                 16
## 108                          54           39                 10
## 109                          61           NA                 NA
## 110                          57           23                 10
## 111                          53           24                 16
## 112                          47           20                 10
## 113                          60           30                 16
## 114                          55           25                 14
## 115                          58           31                 16
## 116                          57           37                 14
## 117                          53           29                 16
## 118                          48           NA                 NA
## 119                          66           30                 12
## 120                          61           21                 10
## 121                          50           20                 10
## 122                          45           26                 16
## 123                          57           NA                 NA
## 124                          49           41                 12
## 125                          54           30                 16
## 126                          68           26                 14
## 127                          57           NA                 NA
## 128                          62           27                 14
## 129                          62           37                 14
## 130                          66           46                 NA
## 131                          53           24                 12
## 132                          52           23                 14
## 133                          62           30                 10
## 134                          64           25                 12
## 135                          57           32                 16
## 136                          64           38                 14
## 137                          48           22                 14
## 138                          61           31                 16
## 139                          51           31                 16
## 140                          60           20                 14
## 141                          62           19                 12
## 142                          59           24                 12
## 143                          50           31                 16
## 144                          48           37                 12
## 145                          69           23                 16
## 146                          70           38                 14
## 147                          50           20                 12
## 148                          57           23                 12
## 149                          78           40                 16
## 150                          54           39                 10
## 151                          61           NA                 NA
## 152                          57           23                 10
## 153                          53           24                 16
## 154                          47           20                 10
## 155                          60           30                 16
## 156                          55           25                 14
## 157                          58           31                 16
## 158                          57           37                 14
## 159                          53           29                 16
## 160                          48           NA                 NA
## 161                          66           30                 12
## 162                          61           21                 10
## 163                          50           20                 10
## 164                          45           26                 16
## 165                          57           NA                 NA
## 166                          49           41                 12
## 167                          54           30                 16
## 168                          68           26                 14
##     paternal.cigarettes paternal.height low.birthweight geriatric.pregnancy
## 1                    NA              NA               0               FALSE
## 2                     0             178               0               FALSE
## 3                     0             170               0               FALSE
## 4                     0             175               0                TRUE
## 5                     7             179               0               FALSE
## 6                    25              NA               1               FALSE
## 7                    25             181               0               FALSE
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##     sample.1272 sample.1262 sample.575 sample.792 sample.752 sample.619
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##     sample.57 sample.431 sample.532 sample.223 sample.1107 sample.697
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##     sample.1683 sample.808 sample.822 sample.1016 sample.820 sample.1081
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##     sample.321 sample.1636 sample.1360 sample.1058 sample.755 sample.462
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Visualizations

The ggplot2 library is an extremely popular visualization package that provides an interface for extremely fine control over graphics for plotting. It is used by a number of of other popular packages in their built-in plotting functions. It provides a “grammar of graphics” that is quite useful to know.

A note about accessibility:

The default colors automatically selected by ggplot2 are not very user-friendly. Colors are chosen by sampling evenly spaced hues on the color wheel. Because of this behavior, all of the colors have similar intensity, which means that they do not work well when printed in gray-scale, and may be difficult to distinguish for users with atypical color vision. There are many resources for selecting color palettes online. Here are just a few:

In this documentation we will be using four palettes generated using the viridis library.

library(ggplot2)
library(viridis)
## Loading required package: viridisLite
?viridis
## Help on topic 'viridis' was found in the following packages:
## 
##   Package               Library
##   viridis               /Library/Frameworks/R.framework/Versions/4.3-arm64/Resources/library
##   viridisLite           /Library/Frameworks/R.framework/Versions/4.3-arm64/Resources/library
## 
## 
## Using the first match ...
locations.palette <- viridis(3)
smoking.palette <- inferno(2, begin = 0.5, direction = -1)
years.palette <- mako(2, begin = 0.4, end = 0.9, direction = -1)
genes.palette <- plasma(4)

As we go through this plotting section, we will pause several times to allow you to explore. Don’t limit yourself to the visualizations included! Experiment with manipulating each of the elements of the plots to accheive interesting and informative graphics.

8.1 The ggplot() function

The basic function of the ggplot2 library is ggplot().

?ggplot

It is capable of taking a lot of arguments and options, but requires only two: an object (typically a data frame) containing the data, and a list of “aesthetic mappings” that tell R which values to use for the axes, colors, and other graphical elements of the plot.

ggplot(data = experiment, mapping = aes(x = birthweight))

Alone, this produces an empty plot. The ggplot() function by itself creates the blank canvas upon which the plot will be drawn. The plot elements are added to this canvas in layers called “geoms.”

##8.2 The geom

There are over 30 geoms in the ggplot2 library, each of which accepts a particular set of aesthetic mappings. The geoms inherit the mapping specified in the original ggplot() function call, and additional layer-specific aesthetics may be specified within the geom. Let’s start with one of the simplest geoms, the histogram.

The geom_histogram() function requires, at a minimum, that a value be provided for x.

ggplot(data = experiment, mapping = aes(x = birthweight)) +
  geom_histogram()
## `stat_bin()` using `bins = 30`. Pick better value with `binwidth`.

## `stat_bin()` using `bins = 30`. Pick better value with `binwidth`.

Setting the “binwidth” parameter within the geom_histogram() call changes the appearance of the plot and eliminates the message.

ggplot(data = experiment, mapping = aes(x = birthweight)) +
  geom_histogram(binwidth = 1)

ggplot(data = experiment, mapping = aes(x = birthweight)) +
  geom_histogram(binwidth = 0.25)

8.3 Adding color (and fill) to geoms

The color (for lines and points) and fill (for areas, like bars) of a geom can add another layer of information to the plot.

ggplot(data = experiment, mapping = aes(x = birthweight, fill = location)) +
  geom_histogram(binwidth = 0.25) +
  scale_fill_manual(values = locations.palette) 

Here the total height of the bar is equal to the number of births at each weight, and the fill denotes the hospital at which the birth occured.

##8.4 Creating faceted plots Sometimes, it may be more clear graphically to create multiple sub-plots or “facets” based on categorical values in the data. The facet_wrap() and facet_grid() functions allow the user to break the data down into multiple plots by one or two categorical variables, respectively.

'ggplot(data = experiment, mapping = aes(x = birthweight, fill = location)) +
  geom_histogram(binwidth = 0.25) +
  scale_fill_manual(values = locations.palette) +
  facet_wrap(~year, nrow = 2)'
## [1] "ggplot(data = experiment, mapping = aes(x = birthweight, fill = location)) +\n  geom_histogram(binwidth = 0.25) +\n  scale_fill_manual(values = locations.palette) +\n  facet_wrap(~year, nrow = 2)"
getwd()
## [1] "/Users/syukurhalim/Documents/KULIAH S2/SEMESTER 2/Bioinformatika/PENGANTAR BIOINFORMATIKA (PUBLISH 1)"

8.5 Add and modify labels

Often, the aesthetic names (column names) are uninformative, or unattractive. This may not make much difference when making exploratory figures, but in a report or publication, it is important to have a greater degree of control over the plot title, axis labels, and so on. The labs() function offers the option to set the following labels:

  • any aesthetic that has been set: in this case, x and fill
  • title: main title of the plot
  • subtitle: displayed below the title
  • caption: displayed at the bottom right of the plot by default
  • tag: label that appears at the top left of the plot by default (e.g. 1A)
  • alt, alt_insight: alt text for the plot (used by screen readers)
'ggplot(data = experiment, mapping = aes(x = birthweight, fill = location)) +
  geom_histogram(binwidth = 0.25) +
  scale_fill_manual(values = locations.palette) +
  facet_wrap(~year, nrow = 2) +
  labs(x = "birth weight (kg)",
       fill = "Hospital",
       tag = "fig. 1",
       caption = "Birth weights by year, color-coded by delivery location.",
       alt = "Pair of histograms displaying the distribution of birth weights of infants born at General Hospital, Memorial Hospital, and Silver Hill Medical Center in 1967 and 1968.")'
## [1] "ggplot(data = experiment, mapping = aes(x = birthweight, fill = location)) +\n  geom_histogram(binwidth = 0.25) +\n  scale_fill_manual(values = locations.palette) +\n  facet_wrap(~year, nrow = 2) +\n  labs(x = \"birth weight (kg)\",\n       fill = \"Hospital\",\n       tag = \"fig. 1\",\n       caption = \"Birth weights by year, color-coded by delivery location.\",\n       alt = \"Pair of histograms displaying the distribution of birth weights of infants born at General Hospital, Memorial Hospital, and Silver Hill Medical Center in 1967 and 1968.\")"

8.6 Making global changes to the plot’s appearance using themes

The “theme” of a ggplot object controls the graphical elements that are not mapped onto the data. This includes things like the font size and alignment of axis labels and the color of the plot background. There are a number of pre-set themes offering a range of plot styles.

In addition, the theme() function offers access to these graphical elements independently. Using it, you can change the angle of the text on the x axis, the placement of the legend, and many other things. Here, we will use it to remove the legend title.

'ggplot(data = experiment, mapping = aes(x = birthweight, fill = location)) +
  geom_histogram(binwidth = 0.25) +
  scale_fill_manual(values = locations.palette) +
  facet_grid(location~year) +
  labs(x = "weight (kg)", y = "births") +
  theme_bw() +
  theme(legend.title = element_blank())'
## [1] "ggplot(data = experiment, mapping = aes(x = birthweight, fill = location)) +\n  geom_histogram(binwidth = 0.25) +\n  scale_fill_manual(values = locations.palette) +\n  facet_grid(location~year) +\n  labs(x = \"weight (kg)\", y = \"births\") +\n  theme_bw() +\n  theme(legend.title = element_blank())"

An interesting alternative to geom_histogram() is geom_density().

ggplot(data = experiment, mapping = aes(x = birthweight, fill = smoker)) +
  geom_density(alpha = 0.5) +
  scale_fill_manual(values = smoking.palette) +
  labs(x = "birth weight (kg)", fill = "Maternal smoking in pregnancy") +
  theme_bw()

The alpha channel of a color determines its opacity. In this case, we have placed alpha = 0.5 outside of the aes() function call, because the opacity is not being mapped to any characteristic of the data.

The examples above use color in a discrete fashion. In the scatter plot below, the color of each point represents a birth weight (continuous variable).

'experiment %>%
  filter(smoker == TRUE) %>%
  ggplot(mapping = aes(x = maternal.cigarettes,
                       y = weeks.gestation,
                       color = birthweight)) +
  geom_point()  +
  labs(x = "Maternal cigarettes / day", y = "Gestational age at birth (weeks)", color = "Birth weight (kg)") +
  scale_color_viridis(option = "inferno", begin = 0.4) +
  theme_bw()'
## [1] "experiment %>%\n  filter(smoker == TRUE) %>%\n  ggplot(mapping = aes(x = maternal.cigarettes,\n                       y = weeks.gestation,\n                       color = birthweight)) +\n  geom_point()  +\n  labs(x = \"Maternal cigarettes / day\", y = \"Gestational age at birth (weeks)\", color = \"Birth weight (kg)\") +\n  scale_color_viridis(option = \"inferno\", begin = 0.4) +\n  theme_bw()"

Notice that it is not necessary to specify a color palette; viridis has a built-in function for interacting with ggplot objects.

8.7 Change the labels for categorical values

All of the elements of the plot, not just the height of bars and position of points, convey information. The labels on a categorical axis should be meaningful.

'experiment %>%
  ggplot(mapping = aes(x = smoker, y = birthweight, fill = smoker)) +
  geom_boxplot() +
  scale_fill_manual(values = smoking.palette) +
  theme_bw()'
## [1] "experiment %>%\n  ggplot(mapping = aes(x = smoker, y = birthweight, fill = smoker)) +\n  geom_boxplot() +\n  scale_fill_manual(values = smoking.palette) +\n  theme_bw()"

The bar chart above displays the values “TRUE” and “FALSE” on the x-axis. This is fine for an exploratory plot, but in a report or publication, it might be more informative to replace them with “smoker” and “non-smoker.” The scale_x_discrete() function allows us to do just that.

'experiment %>%
  ggplot(mapping = aes(x = smoker, y = birthweight, fill = smoker)) +
  geom_boxplot() +
  scale_fill_manual(values = smoking.palette) +
  scale_x_discrete(labels = c("non-smoker", "smoker")) +
  guides(fill = "none") +
  labs(y = "birth weight (kg)", x = "maternal cigarette use in pregnancy") +
  theme_bw()'
## [1] "experiment %>%\n  ggplot(mapping = aes(x = smoker, y = birthweight, fill = smoker)) +\n  geom_boxplot() +\n  scale_fill_manual(values = smoking.palette) +\n  scale_x_discrete(labels = c(\"non-smoker\", \"smoker\")) +\n  guides(fill = \"none\") +\n  labs(y = \"birth weight (kg)\", x = \"maternal cigarette use in pregnancy\") +\n  theme_bw()"

## 8.8 Change the direction of axes to improve readability

If the category names are very long, it may be necessary to change the direction of the axis labels.

'experiment %>%
  ggplot(mapping = aes(x = smoker, y = birthweight, fill = smoker)) +
  geom_boxplot() +
  scale_fill_manual(values = smoking.palette) +
  scale_x_discrete(labels = c("non-smoking in pregnancy", "cigarette smoking in pregnancy")) +
  guides(fill = "none") +
  labs(y = "birth weight (kg)") +
  theme_bw() +
  theme(axis.title.x = element_blank(),
        axis.text.x = element_text(angle = 90, vjust = 0.5, hjust = 1))'
## [1] "experiment %>%\n  ggplot(mapping = aes(x = smoker, y = birthweight, fill = smoker)) +\n  geom_boxplot() +\n  scale_fill_manual(values = smoking.palette) +\n  scale_x_discrete(labels = c(\"non-smoking in pregnancy\", \"cigarette smoking in pregnancy\")) +\n  guides(fill = \"none\") +\n  labs(y = \"birth weight (kg)\") +\n  theme_bw() +\n  theme(axis.title.x = element_blank(),\n        axis.text.x = element_text(angle = 90, vjust = 0.5, hjust = 1))"

Or even the direction of the axes themselves.

'experiment %>%
  ggplot(mapping = aes(x = smoker, y = birthweight, fill = smoker)) +
  geom_boxplot() +
  scale_fill_manual(values = smoking.palette) +
  scale_x_discrete(labels = c("non-smoking in pregnancy", "cigarette smoking in pregnancy")) +
  guides(fill = "none") +
  labs(y = "birth weight (kg)") +
  coord_flip() +
  theme_bw() +
  theme(axis.title.y = element_blank())'
## [1] "experiment %>%\n  ggplot(mapping = aes(x = smoker, y = birthweight, fill = smoker)) +\n  geom_boxplot() +\n  scale_fill_manual(values = smoking.palette) +\n  scale_x_discrete(labels = c(\"non-smoking in pregnancy\", \"cigarette smoking in pregnancy\")) +\n  guides(fill = \"none\") +\n  labs(y = \"birth weight (kg)\") +\n  coord_flip() +\n  theme_bw() +\n  theme(axis.title.y = element_blank())"

##8.9 The pivot_longer() function revisited

In the previous section on Tidyverse, pivot_longer() was only briefly discussed. This is because the easiest way to explain why changing the shape of a data set to create fewer, longer columns is useful is to demonstrate.

'experiment %>%
  pivot_longer(cols = c(22:25),
               names_to = "gene.id",
               values_to = "expression") %>%
  ggplot(mapping = aes(x = smoker, y = expression, fill = smoker)) +
  geom_violin() +
  scale_fill_manual(values = smoking.palette) +
  scale_x_discrete(labels = c("non-smoker", "smoker")) +
  facet_wrap(~ gene.id, nrow = 2) +
  guides(fill = "none") +
  labs(x = "Maternal tobacco use in pregnancy", y = "miRNA expression") +
  theme_bw()'
## [1] "experiment %>%\n  pivot_longer(cols = c(22:25),\n               names_to = \"gene.id\",\n               values_to = \"expression\") %>%\n  ggplot(mapping = aes(x = smoker, y = expression, fill = smoker)) +\n  geom_violin() +\n  scale_fill_manual(values = smoking.palette) +\n  scale_x_discrete(labels = c(\"non-smoker\", \"smoker\")) +\n  facet_wrap(~ gene.id, nrow = 2) +\n  guides(fill = \"none\") +\n  labs(x = \"Maternal tobacco use in pregnancy\", y = \"miRNA expression\") +\n  theme_bw()"

After pivoting the miRNA data into a single column called expression, that data is available for ggplot to map that onto an aesthetic property.

##8.10 Use shape and line type to convey meaning

Color is not the only aesthetic available for mapping to the data. Shape and line type are also good candidates for displaying categorical (or discrete) values.

'experiment %>%
  pivot_longer(cols = c(22:25),
               names_to = "gene.id",
               values_to = "expression") %>%
  ggplot(mapping = aes(x = maternal.cigarettes, y = expression, color = location, shape = low.birthweight)) +
  geom_point() +
  scale_color_manual(values = locations.palette) +
  scale_shape_manual(values = c(16, 17), labels = c("< 2.72 kg", ">= 2.72 kg")) +
  facet_wrap(~ gene.id, nrow = 2) +
  labs(x = "Maternal cigarettes / day", y = "miRNA expression") +
  theme_bw() +
  theme(legend.title = element_blank())'
## [1] "experiment %>%\n  pivot_longer(cols = c(22:25),\n               names_to = \"gene.id\",\n               values_to = \"expression\") %>%\n  ggplot(mapping = aes(x = maternal.cigarettes, y = expression, color = location, shape = low.birthweight)) +\n  geom_point() +\n  scale_color_manual(values = locations.palette) +\n  scale_shape_manual(values = c(16, 17), labels = c(\"< 2.72 kg\", \">= 2.72 kg\")) +\n  facet_wrap(~ gene.id, nrow = 2) +\n  labs(x = \"Maternal cigarettes / day\", y = \"miRNA expression\") +\n  theme_bw() +\n  theme(legend.title = element_blank())"

There are 25 shapes available for plotting. You can see them all here.

'experiment %>%
  ggplot(mapping = aes(x = weeks.gestation, y = birthweight, linetype = smoker)) +
  geom_quantile(quantiles = c(0.25, 0.5, 0.75), color = "black") +
  labs(x = "gestational age at birth", y = "birth weight (kg)", linetype = "geriatric preganancy") +
  theme_bw()'
## [1] "experiment %>%\n  ggplot(mapping = aes(x = weeks.gestation, y = birthweight, linetype = smoker)) +\n  geom_quantile(quantiles = c(0.25, 0.5, 0.75), color = \"black\") +\n  labs(x = \"gestational age at birth\", y = \"birth weight (kg)\", linetype = \"geriatric preganancy\") +\n  theme_bw()"

##8.11 Focus in on one portion of the plot

We can zoom in to the the lower left hand corner of the plot using coord_cartesian().

'experiment %>%
  ggplot(mapping = aes(x = weeks.gestation, y = birthweight, linetype = smoker)) +
  geom_quantile(quantiles = c(0.25, 0.5, 0.75), color = "black") +
  labs(x = "gestational age at birth", y = "birth weight (kg)", linetype = "geriatric preganancy") +
  coord_cartesian(xlim = c(33, 35), ylim = c(1.5, 3.5)) +
  theme_bw()'
## [1] "experiment %>%\n  ggplot(mapping = aes(x = weeks.gestation, y = birthweight, linetype = smoker)) +\n  geom_quantile(quantiles = c(0.25, 0.5, 0.75), color = \"black\") +\n  labs(x = \"gestational age at birth\", y = \"birth weight (kg)\", linetype = \"geriatric preganancy\") +\n  coord_cartesian(xlim = c(33, 35), ylim = c(1.5, 3.5)) +\n  theme_bw()"

##8.12 Layer multiple geoms

It is not necessary to make a single geom convey all of the information a plot must communicate. Instead, ggplot2 offers users the ability to layer geoms together. As long as they use the same axes, geoms may share a plot.

ggplot(experiment, mapping = aes(x = weeks.gestation,
                                 y = birthweight,
                                 color = smoker)) +
  geom_point() +
  geom_smooth(alpha = 0.2) +
  labs(x = "Gestational age at birth (weeks)",
       y = "Birth weight (kg)",
       color = "Maternal tobacco use",
       caption = "Birthweight increases with gestational age for infants born to both\nsmokers and non-smokers.") +
  scale_color_manual(values = smoking.palette) +
  theme_bw() +
  theme(plot.caption = element_text(hjust = 0))
## `geom_smooth()` using method = 'loess' and formula = 'y ~ x'

'experiment %>%
  group_by(year, smoker) %>%
  summarise(mean.birthweight = mean(birthweight),
            sd.birthweight = sd(birthweight)) %>%
  ggplot(mapping = aes(x = as.factor(year), y = mean.birthweight, fill = smoker)) +
  geom_col(position = position_dodge2(preserve = "single")) +
  geom_errorbar(mapping = aes(ymin = mean.birthweight - sd.birthweight,
                              ymax = mean.birthweight + sd.birthweight),
                position = position_dodge2(preserve = "single")) +
  scale_fill_manual(values = smoking.palette) +
  labs(x = "birth year", y = "birth weight (kg)", fill = "maternal smoking") +
  theme_bw()'
## [1] "experiment %>%\n  group_by(year, smoker) %>%\n  summarise(mean.birthweight = mean(birthweight),\n            sd.birthweight = sd(birthweight)) %>%\n  ggplot(mapping = aes(x = as.factor(year), y = mean.birthweight, fill = smoker)) +\n  geom_col(position = position_dodge2(preserve = \"single\")) +\n  geom_errorbar(mapping = aes(ymin = mean.birthweight - sd.birthweight,\n                              ymax = mean.birthweight + sd.birthweight),\n                position = position_dodge2(preserve = \"single\")) +\n  scale_fill_manual(values = smoking.palette) +\n  labs(x = \"birth year\", y = \"birth weight (kg)\", fill = \"maternal smoking\") +\n  theme_bw()"

Refrensi :

https://ucdavis-bioinformatics-training.github.io/2022_February_Introduction_to_R_for_Bioinformatics/visualizations.html