---

title: “Pseudotime analysis” output: html_notebook Athors:Dr Upasna Srivastava

library(Seurat)

## The legacy packages maptools, rgdal, and rgeos, underpinning the sp package,
## which was just loaded, will retire in October 2023.
## Please refer to R-spatial evolution reports for details, especially
## https://r-spatial.org/r/2023/05/15/evolution4.html.
## It may be desirable to make the sf package available;
## package maintainers should consider adding sf to Suggests:.
## The sp package is now running under evolution status 2
##      (status 2 uses the sf package in place of rgdal)

## Attaching SeuratObject

library(SeuratDisk)

## Registered S3 method overwritten by 'SeuratDisk':
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##   as.sparse.H5Group Seurat

library(SeuratWrappers)
library(monocle3)

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##     colnames, dirname, do.call, duplicated, eval, evalq, Filter, Find,
##     get, grep, grepl, intersect, is.unsorted, lapply, Map, mapply,
##     match, mget, order, paste, pmax, pmax.int, pmin, pmin.int,
##     Position, rank, rbind, Reduce, rownames, sapply, setdiff, sort,
##     table, tapply, union, unique, unsplit, which.max, which.min

## Welcome to Bioconductor
## 
##     Vignettes contain introductory material; view with
##     'browseVignettes()'. To cite Bioconductor, see
##     'citation("Biobase")', and for packages 'citation("pkgname")'.

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## Attaching package: 'MatrixGenerics'

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##     rowSdDiffs, rowSds, rowSums2, rowTabulates, rowVarDiffs, rowVars,
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## Attaching package: 'monocle3'

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library(dplyr)

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## Attaching package: 'dplyr'

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