Two Sample t-Test

Example with Wide Format Data

# read in our data (wide format) 
Cd.BeetBarley<- data.frame(
  redbeet= c(18, 5, 10, 8, 16, 12, 8, 8, 11, 5, 6, 8, 9, 21, 9), 
  barley= c(8, 5, 10, 19, 15, 18, 11, 8, 9, 4, 5, 13, 7, 5, 7))
# three ways to look at the data structure
str(Cd.BeetBarley)
'data.frame':   15 obs. of  2 variables:
 $ redbeet: num  18 5 10 8 16 12 8 8 11 5 ...
 $ barley : num  8 5 10 19 15 18 11 8 9 4 ...
summary(Cd.BeetBarley) 
    redbeet          barley    
 Min.   : 5.00   Min.   : 4.0  
 1st Qu.: 8.00   1st Qu.: 6.0  
 Median : 9.00   Median : 8.0  
 Mean   :10.27   Mean   : 9.6  
 3rd Qu.:11.50   3rd Qu.:12.0  
 Max.   :21.00   Max.   :19.0  
head(Cd.BeetBarley) 
  redbeet barley
1      18      8
2       5      5
3      10     10
4       8     19
5      16     15
6      12     18
with(Cd.BeetBarley,boxplot(redbeet,barley,
     col= "lightgrey",   
     main= "Phytoremediation Efficiency of Crop Plants",
     xlab= "Crop type", ylab= "Cadmium reduction (%)", 
     names= c("Redbeet","Barley"),
     ylim=  c(0,25), las= 1,
     boxwex=0.6)) 

with(Cd.BeetBarley, var.test(redbeet, barley))

    F test to compare two variances

data:  redbeet and barley
F = 0.97888, num df = 14, denom df = 14, p-value = 0.9687
alternative hypothesis: true ratio of variances is not equal to 1
95 percent confidence interval:
 0.3286377 2.9156685
sample estimates:
ratio of variances 
         0.9788762 
with(Cd.BeetBarley, t.test(redbeet, barley, var.equal = TRUE))

    Two Sample t-test

data:  redbeet and barley
t = 0.38658, df = 28, p-value = 0.702
alternative hypothesis: true difference in means is not equal to 0
95 percent confidence interval:
 -2.865852  4.199185
sample estimates:
mean of x mean of y 
 10.26667   9.60000 

Example with Long Format Data

# read in our data (long format)
Cd.CabbageMaize <- data.frame(remed.pcnt = c(46, 50, 44, 44, 43, 52, 48, 24, 51, 
    29, 53, 32, 61, 59, 35, 34, 26, 44, 17, 34, 19, 34, 34, 43, 18, 34, 27, 27, 53, 
    30), plt.typ = c(rep("cabbage", times = 15), rep("maize", times = 15)))# read in our data (long format)
Cd.CabbageMaize <- data.frame(remed.pcnt = c(46, 50, 44, 44, 43, 52, 48, 24, 51, 
    29, 53, 32, 61, 59, 35, 34, 26, 44, 17, 34, 19, 34, 34, 43, 18, 34, 27, 27, 53, 
    30), plt.typ = c(rep("cabbage", times = 15), rep("maize", times = 15)))
# get summary & check data structure
str(Cd.CabbageMaize)
'data.frame':   30 obs. of  2 variables:
 $ remed.pcnt: num  46 50 44 44 43 52 48 24 51 29 ...
 $ plt.typ   : chr  "cabbage" "cabbage" "cabbage" "cabbage" ...
summary(Cd.CabbageMaize)
   remed.pcnt      plt.typ         
 Min.   :17.00   Length:30         
 1st Qu.:29.25   Class :character  
 Median :34.50   Mode  :character  
 Mean   :38.17                     
 3rd Qu.:47.50                     
 Max.   :61.00                     
head(Cd.CabbageMaize)
  remed.pcnt plt.typ
1         46 cabbage
2         50 cabbage
3         44 cabbage
4         44 cabbage
5         43 cabbage
6         52 cabbage
# Note we don't NEED to give names for boxes if the data is in long format
# This is an advantage of the long format approach
with(Cd.CabbageMaize,boxplot(remed.pcnt~plt.typ, 
     col= "lightgrey",                          
     main= "Phytoremediation Efficiency of Crop Plants",
     xlab= "Crop type", ylab= "Cadmium reduction (%)",
     ylim=  c(10,70), las= 1, boxwex=.6)) 

with(Cd.CabbageMaize, var.test(remed.pcnt ~ plt.typ))  # long format, use ~ not ,

    F test to compare two variances

data:  remed.pcnt by plt.typ
F = 1.1449, num df = 14, denom df = 14, p-value = 0.8037
alternative hypothesis: true ratio of variances is not equal to 1
95 percent confidence interval:
 0.3843653 3.4100823
sample estimates:
ratio of variances 
          1.144866 
with(Cd.CabbageMaize, t.test(remed.pcnt ~ plt.typ, var.equal = TRUE))

    Two Sample t-test

data:  remed.pcnt by plt.typ
t = 3.4687, df = 28, p-value = 0.00171
alternative hypothesis: true difference in means between group cabbage and group maize is not equal to 0
95 percent confidence interval:
  5.377502 20.889165
sample estimates:
mean in group cabbage   mean in group maize 
             44.73333              31.60000