Last updated: 2023-02-22

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Knit directory: oNKo_automation_dev/

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Aim

The aim of this test is to assess how consistent that Varispan arm on Janus G3 under the revised automation workflow for oNKo

Researcher

ML

Readout

Absorbance of tartrazine at 410 on Cytation C10 plate reader

Required R packages

data.table, DT, platetools, reshape2, tidyverse, patchwork

# load the required packages
library(data.table)
library(DT)
library(platetools)
library(reshape2)
library(readxl)
library(tidyverse)
library(patchwork)
library(here)
library(htmltools)


# check if here() correctly identified the directory
here::here()
[1] "/home/mli/ML_oNKo/230220_143052_230220_ML_oNKo_Sanitycheck/oNKo_automation_dev"
# set the file prefix

prefix <- "ML_oNKo_dev"

# def funct
read_excel_allsheets <- function(filename, tibble = FALSE) {
    sheets <- readxl::excel_sheets(filename)
    x <- lapply(sheets, function(X) readxl::read_excel(filename, sheet = X, skip = 24))
    if (!tibble)
        x <- lapply(x, as.data.frame)
    names(x) <- sheets
    x
}

Details

Test date (yyyy-mm-dd)

2023-02-22

Tartrazine concentration

Tartrazine stock at 12.5 ug/ul

Data cleaning

The raw data was read into R Studio.

Delivery Consistency


Comments: To save $ and time, I did one round of dispensing to half of the plate. To get accuracy measure, I will have to have a standard curve, and do multiple dispensing rounds, using the delta to estimate accuracy.


Abs values

Abs tables


Check each tip



 

Analysed by Mark Li

Victorian Centre for Functional Genomics

 


sessionInfo()
R version 4.2.0 (2022-04-22)
Platform: x86_64-pc-linux-gnu (64-bit)
Running under: CentOS Linux 7 (Core)

Matrix products: default
BLAS:   /usr/lib64/libblas.so.3.4.2
LAPACK: /usr/lib64/liblapack.so.3.4.2

locale:
 [1] LC_CTYPE=en_AU.UTF-8       LC_NUMERIC=C              
 [3] LC_TIME=en_AU.UTF-8        LC_COLLATE=en_AU.UTF-8    
 [5] LC_MONETARY=en_AU.UTF-8    LC_MESSAGES=en_AU.UTF-8   
 [7] LC_PAPER=en_AU.UTF-8       LC_NAME=C                 
 [9] LC_ADDRESS=C               LC_TELEPHONE=C            
[11] LC_MEASUREMENT=en_AU.UTF-8 LC_IDENTIFICATION=C       

attached base packages:
[1] stats     graphics  grDevices utils     datasets  methods   base     

other attached packages:
 [1] htmltools_0.5.4   here_1.0.1        patchwork_1.1.2   forcats_1.0.0    
 [5] stringr_1.5.0     dplyr_1.1.0       purrr_1.0.1       readr_2.1.4      
 [9] tidyr_1.3.0       tibble_3.1.8      ggplot2_3.4.1     tidyverse_1.3.2  
[13] readxl_1.4.2      reshape2_1.4.4    platetools_0.1.5  DT_0.27          
[17] data.table_1.14.8 workflowr_1.7.0  

loaded via a namespace (and not attached):
 [1] fs_1.6.1            lubridate_1.9.2     bit64_4.0.5        
 [4] RColorBrewer_1.1-3  httr_1.4.4          rprojroot_2.0.3    
 [7] tools_4.2.0         backports_1.4.1     bslib_0.4.2        
[10] utf8_1.2.3          R6_2.5.1            DBI_1.1.3          
[13] colorspace_2.1-0    withr_2.5.0         tidyselect_1.2.0   
[16] processx_3.8.0      bit_4.0.5           compiler_4.2.0     
[19] git2r_0.31.0        cli_3.6.0           rvest_1.0.3        
[22] formatR_1.14        xml2_1.3.3          labeling_0.4.2     
[25] sass_0.4.5          scales_1.2.1        callr_3.7.3        
[28] digest_0.6.31       rmarkdown_2.20      pkgconfig_2.0.3    
[31] highr_0.10          dbplyr_2.3.0        fastmap_1.1.0      
[34] htmlwidgets_1.6.1   rlang_1.0.6         rstudioapi_0.14    
[37] farver_2.1.1        jquerylib_0.1.4     generics_0.1.3     
[40] jsonlite_1.8.4      crosstalk_1.2.0     vroom_1.6.1        
[43] googlesheets4_1.0.1 magrittr_2.0.3      Rcpp_1.0.10        
[46] munsell_0.5.0       fansi_1.0.4         lifecycle_1.0.3    
[49] stringi_1.7.12      whisker_0.4.1       yaml_2.3.7         
[52] snakecase_0.11.0    plyr_1.8.8          grid_4.2.0         
[55] parallel_4.2.0      promises_1.2.0.1    crayon_1.5.2       
[58] haven_2.5.1         hms_1.1.2           knitr_1.42         
[61] ps_1.7.2            pillar_1.8.1        reprex_2.0.2       
[64] glue_1.6.2          evaluate_0.20       getPass_0.2-2      
[67] modelr_0.1.10       vctrs_0.5.2         tzdb_0.3.0         
[70] httpuv_1.6.9        cellranger_1.1.0    gtable_0.3.1       
[73] assertthat_0.2.1    cachem_1.0.6        xfun_0.37          
[76] janitor_2.2.0       broom_1.0.3         later_1.3.0        
[79] googledrive_2.0.0   gargle_1.3.0        timechange_0.2.0   
[82] ellipsis_0.3.2